CN114149917A

CN114149917A - Integrated living cell workstation

Info

Publication number: CN114149917A
Application number: CN202111349423.0A
Authority: CN
Inventors: 苗春光; 胡浙平; 刘需蛟
Original assignee: Anhui Luohua Biotechnology Co ltd
Current assignee: Anhui Luohua Biotechnology Co ltd
Priority date: 2021-11-15
Filing date: 2021-11-15
Publication date: 2022-03-08

Abstract

The invention discloses an integrated living cell workstation, which comprises a box body, wherein a culture chamber for placing a culture instrument and a premixing air chamber are arranged in the box body, the premixing air chamber is respectively connected with a carbon dioxide air pipe and a nitrogen pipe and is provided with a micro air flow valve, an air inlet pipe and a return pipe are communicated between the premixing air chamber and the culture chamber, and a gas concentration sensor is also arranged in the premixing air chamber. The premixing air chamber is provided with oxygen and carbon dioxide gas concentration sensors for detecting the concentration of each component gas in the cabin, and the corresponding micro air flow valve can be controlled to perform air inlet action according to the indication parameters of the gas concentration sensors, so that the environment required by normal growth and reproduction of cells is provided. Meanwhile, the requirements of researchers for observation and photographing are met.

Description

Integrated living cell workstation

Technical Field

The invention belongs to the technical field of biological experiment equipment, and particularly relates to an integrated living cell workstation.

Background

Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.

The existing cell micro-culture has very strict requirements on gaseous environment in the cell culture process, and the prior art cannot accurately control the gas concentration of the gaseous environment of the cell culture, so that the gaseous environment of the cell culture has floatability, and the normal growth of cells is influenced by small gas concentration floating. Therefore, the invention creates a living cell workstation for cell culture, which not only provides a stable culture space, but also creates a proper environmental gas concentration for cell culture, and solves the adverse effect caused by the change of gaseous environment in the cell culture process.

Disclosure of Invention

The invention aims to provide an integrated living cell workstation, which solves the adverse effect caused by the change of gaseous environment in the cell culture process.

In order to solve the technical problems, the invention is realized by the following technical scheme:

an integrated living cell workstation comprises a box body with a closed shell, wherein a culture chamber for placing a culture instrument and a premixing air chamber are arranged in the box body;

the system comprises a pre-mixing air chamber, a culture chamber, a carbon dioxide gas pipe, a nitrogen pipe, a micro air flow valve, an overflow exhaust pipe, an air inlet pipe and a return pipe, wherein the pre-mixing air chamber is connected with the carbon dioxide gas pipe and the nitrogen pipe which are used for supplementing carbon dioxide and nitrogen respectively;

and a gas concentration sensor for detecting the concentrations of oxygen and carbon dioxide is also arranged in the premixing gas chamber.

Furthermore, a circuit controller is further installed in the box body, a touch display screen is installed on the side wall of the box body, the circuit controller monitors the gas concentration of the premixing gas chamber through a concentration sensor, and then the micro airflow valves corresponding to the carbon dioxide gas pipe and the nitrogen gas pipe are controlled to supplement.

Furthermore, the part of the air inlet pipe corresponding to the premixing air chamber is connected in series with a variable flow air pump and a flow sensor.

Further, a stirring blade for mixing gas is arranged in the uniform gas mixing chamber.

Furthermore, an imaging lens is arranged above the culture chamber corresponding to the culture device.

Further, the imaging lens is provided with an X-axis moving guide rail and a Y-axis moving guide rail.

Further, the culture utensil comprises a culture dish, and a constant-temperature heating plate for supporting the culture dish is installed in the culture chamber.

Furthermore, the air inlet pipe and the return pipe are oppositely communicated with the side wall of the culture chamber, and an air distribution chamber is arranged at the communication position of the culture chamber corresponding to the air inlet pipe.

Further, the gas distribution chamber is assembled by the culture chamber lateral wall and the separation net and is formed, a rotatable main pipe is installed between the separation net central part and the culture chamber lateral wall, the main pipe lateral wall is communicated with a plurality of branch pipes, the air outlet holes are formed in the branch pipes, and the main pipe is communicated with the air inlet pipe in a sleeved mode.

Furthermore, the culture chamber is provided with a motor for driving the main pipe to rotate, the main pipe is sleeved with a fluted disc, and the output end of the motor is provided with a gear which is in meshing transmission with the fluted disc.

The invention has the following beneficial effects:

(1) the invention relates to a gas supply balance environment device which is built under the condition of simulating the environment in an organism, a carbon dioxide gas pipe and a nitrogen pipe are arranged in a premixing air chamber, a premixing air chamber for supplying the required gas environment is specially arranged in a culture chamber, meanwhile, the supplement is a circulating supplement, the premixing air chamber and the culture chamber exchange gas constantly, and the gas in the culture chamber and the premixing air chamber is maintained in the required gas concentration range.

(2) The gas pre-mixing chamber is internally provided with oxygen and carbon dioxide gas concentration sensors for detecting the concentration of each component gas in the cabin, and the parts of the carbon dioxide gas pipe and the nitrogen gas pipe corresponding to the gas pre-mixing chamber are respectively provided with a micro air flow valve, which belongs to a controllable valve. At this time, the corresponding micro airflow valve can be controlled to perform air intake operation according to the indication parameter of the gas concentration sensor. Provides the environment for the normal growth and propagation of the cells. Meanwhile, the requirements of researchers for observation and photographing are met.

Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1: the invention is a schematic structural plan view.

FIG. 2: the structure of the invention is shown in the front schematic view.

FIG. 3: the structure of the culture chamber is schematically shown.

In the drawings, the components represented by the respective reference numerals are listed below:

the device comprises a box body 1, a culture apparatus 31, a culture chamber 3, a premixing air chamber 2, a carbon dioxide air pipe 21, a nitrogen pipe 22, a micro air flow valve 24, an overflow exhaust pipe 23, an air inlet pipe 4, a return pipe 32, a gas concentration sensor 25, a touch display screen 12, a variable flow air pump 41, a flow sensor 42, a stirring blade 26, an imaging lens 5, an X-axis moving guide rail 51, a Y-axis moving guide rail 52, a constant temperature heating plate 33, an air distribution chamber 6, a separation net 61, a header pipe 7, a branch pipe 71, a motor 8, a fluted disc 72 and a gear 81.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "opening," "upper," "lower," "thickness," "top," "middle," "length," "inner," "peripheral," and the like are used in an orientation or positional relationship that is merely for convenience in describing and simplifying the description, and do not indicate or imply that the referenced component or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the present invention.

As shown in fig. 1: an integrated living cell workstation comprises a box body 1 with a closed shell, wherein a culture chamber 3 for placing a culture apparatus 31 and a premixing air chamber 2 are arranged in the box body 1;

the premixing air chamber 2 is respectively connected with a carbon dioxide air pipe 21 and a nitrogen pipe 22 for supplementing carbon dioxide and nitrogen, the parts of the carbon dioxide air pipe 21 and the nitrogen pipe 22 corresponding to the premixing air chamber 2 are respectively provided with a micro air flow valve 24, the premixing air chamber 2 is communicated with an overflow exhaust pipe 23, and an air inlet pipe 4 and a return pipe 32 are communicated between the premixing air chamber 2 and the culture chamber 3;

a gas concentration sensor 25 for detecting the concentration of oxygen and carbon dioxide is also installed in the premixing gas chamber 2.

The invention relates to a gas supply balance environment device constructed under the condition of simulating the environment in an organism, which is provided with a premixed gas chamber and a culture chamber, wherein a culture device in the culture chamber can be used for placing culture solution and culturing cells, tissues and the like in the culture solution.

The concentration of oxygen and carbon dioxide in the cultured cells or tissues is required to be high, and generally, the concentration of oxygen in the culture environment needs to be maintained at 5-8% and the concentration of carbon dioxide in the culture environment needs to be maintained at about 5%, so that a good premixing chamber is required to adjust the concentration ratio of the gas so that the concentration of oxygen or carbon dioxide in the supplied gas environment meets the set requirement.

The carbon dioxide air pipe and the nitrogen pipe are arranged in the premixing air chamber, the premixing air chamber is opened to be communicated with a normal air environment in the working process, the concentration of oxygen in normal atmosphere is about 20%, the concentration of carbon dioxide is only 0.03-0.04%, the index difference between the carbon dioxide and the required content index of a gaseous environment is large, therefore, nitrogen needs to be introduced at the same time for discharging oxygen and reducing the concentration of oxygen to a preset range, carbon dioxide is introduced for improving the concentration of carbon dioxide, the culture chamber is specially provided with the premixing air chamber for supplying the required gas environment, meanwhile, the supplement is a circulating supplement, the premixing air chamber and the culture chamber exchange gas constantly, and the gas in the culture chamber and the premixing air chamber is maintained in the required gas concentration range.

The premixing gas chamber is internally provided with oxygen and carbon dioxide gas concentration sensors for detecting the concentration of each component gas in the cabin, and has an important coordination effect on adjusting the initial concentration of the oxygen and the carbon dioxide in the premixing gas chamber at the beginning stage so as to enable the two gases to reach the optimal concentration values.

The parts of the carbon dioxide air pipe and the nitrogen pipe corresponding to the premixing air chamber are provided with miniature airflow valves, which belong to controllable valves. At this time, according to the indication parameter of the gas concentration sensor, the corresponding micro gas flow valve can be controlled to perform gas inlet action, so as to adjust the gas concentration in the cabin and keep the gas balance.

As shown in fig. 1: the box body 1 is also provided with a circuit controller 11, the side wall of the box body 1 is provided with a touch display screen 12, the circuit controller 11 monitors the gas concentration of the premixing air chamber 2 through a concentration sensor 25, and then controls the micro airflow valves 24 corresponding to the carbon dioxide air pipe 21 and the nitrogen air pipe 22 to supplement. The concentration range of the concentration sensor is preset on the premise of tissue and cell culture, then the circuit controller is controlled intelligently without manual real-time monitoring, and when the gas concentration of the premixing gas chamber reaches a preset value, the circuit controller automatically closes the corresponding micro airflow valve. When the gas concentration of the premixing air chamber changes in a floating mode and is out of a normal range, the circuit controller automatically controls the corresponding micro airflow valve to perform gas balance.

As shown in fig. 1: the part of the air inlet pipe 4 corresponding to the premixing air chamber 2 is connected in series with a variable flow air pump 41 and a flow sensor 42. The variable flow air pump is used for enabling the air in the air inlet pipe to have certain power when entering the culture chamber, the air circulation between the two air chambers is positively promoted, the flow sensor can sense the flow condition of the air in the air inlet pipe in real time, and the working power of the variable flow air pump can be properly adjusted according to the flow condition of the air.

As shown in fig. 1: the gas homogenizing and mixing chamber 2 is provided with a stirring blade 26 for mixing gas. The gas mixing device is used for fully mixing the gas in the pre-mixing gas chamber, and is beneficial to more accurate detection data of the concentration sensor.

As shown in fig. 1: the culture chamber 3 is provided with an imaging lens 5 above the culture device 31. The system is used for recording the culture condition of the culture instrument, and meanwhile, the data of the imaging lens can be transmitted to the touch display screen for storage and used for recording the data.

As shown in fig. 1: the imaging lens 5 is mounted with an X-axis moving guide 51 and a Y-axis moving guide 52. The position of the imaging lens can be adjusted as required, and real-time observation and photographing can be achieved.

As shown in fig. 1: the culture device 31 comprises a culture dish, and a constant temperature heating plate 33 for supporting the culture dish 31 is installed in the culture chamber 3. The stability for the constant petri dish was 37 ℃.

As shown in fig. 1: the air inlet pipe 4 and the return pipe 32 are oppositely communicated with the side wall of the culture chamber 3, and an air distribution chamber 6 is arranged at the communication position of the culture chamber 3 corresponding to the air inlet pipe 4. The gas distribution chamber is used for uniformly distributing and dispersing the entering gas. The gas passes through the whole culture chamber and finally flows back to the premixing gas chamber through the return pipe. The gas distribution chamber not only reduces the flow velocity of gas, but also enables the gas concentration of each position of the culture chamber to be more balanced.

As shown in fig. 1: the gas distribution chamber 6 is formed by assembling the side wall of the culture chamber 3 and a separation net 61, a rotatable main pipe 7 is arranged between the center of the separation net 61 and the side wall of the culture chamber 3, and bearings are arranged at the positions of the separation net and the side wall of the culture chamber corresponding to the main pipe. The lateral wall of the header pipe 7 is communicated with a plurality of branch pipes 71, the branch pipes 71 are provided with air outlet holes, and the header pipe 7 is communicated with the air inlet pipe 4 in a sleeved mode. The cooperation of house steward and branch pipe is similar to the gas distribution pipe, with the flow of gas and atmospheric pressure evenly distributed to many, reduces the impact force that atmospheric pressure formed, with the even dispersion of gaseous at the culture room, improves gaseous dwell time at the culture room.

As shown in fig. 1: the culture chamber 6 is provided with a motor 8 for driving the main pipe 7 to rotate, the main pipe 7 is sleeved with a fluted disc 72, and the output end of the motor 8 is provided with a gear 81 which is in meshing transmission with the fluted disc 72. The motor drives the main pipe to rotate, so that each branch pipe rotates by taking the main pipe as an axis, and the gas distribution efficiency is improved.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. An integrated living cell workstation, comprising:

the device comprises a box body (1) with a closed shell, wherein a culture chamber (3) for placing a culture instrument (31) and a premix air chamber (2) are arranged in the box body (1);

the system is characterized in that the premixing air chamber (2) is respectively connected with a carbon dioxide air pipe (21) and a nitrogen pipe (22) for supplementing carbon dioxide and nitrogen, the parts of the carbon dioxide air pipe (21) and the nitrogen pipe (22) corresponding to the premixing air chamber (2) are respectively provided with a micro air flow valve (24), the premixing air chamber (2) is communicated with an overflow exhaust pipe (23), and an air inlet pipe (4) and a return pipe (32) are communicated between the premixing air chamber (2) and the culture chamber (3);

and a gas concentration sensor (25) for detecting the concentrations of oxygen and carbon dioxide is also arranged in the premixing gas chamber (2).

2. The integrated living cell workstation of claim 1, wherein: the gas concentration of the premixing gas chamber (2) is monitored by the circuit controller (11) through a concentration sensor (25), and then the micro airflow valves (24) corresponding to the carbon dioxide gas pipe (21) and the nitrogen gas pipe (22) are controlled to supplement.

3. An integrated living cell workstation according to claim 2, wherein: the part of the air inlet pipe (4) corresponding to the premixing air chamber (2) is connected with a variable flow air pump (41) and a flow sensor (42) in series.

4. An integrated living cell workstation according to any one of claims 1 to 3, wherein: and a stirring blade (26) for mixing gas is arranged in the uniform gas mixing chamber (2).

5. An integrated living cell workstation according to claim 2 or 3, wherein: an imaging lens (5) is arranged above the culture chamber (3) corresponding to the culture utensil (31).

6. An integrated living cell workstation according to claim 5, wherein: the imaging lens (5) is provided with an X-axis moving guide rail (51) and a Y-axis moving guide rail (52).

7. The integrated living cell workstation of claim 1, wherein: the culture utensil (31) comprises a culture dish, and a constant temperature heating plate (33) for supporting the culture dish (31) is installed in the culture chamber (3).

8. The integrated living cell workstation of claim 1, wherein: the air inlet pipe (4) and the return pipe (32) are oppositely communicated with the side wall of the culture chamber (3), and an air distribution chamber (6) is arranged at the communication position of the culture chamber (3) corresponding to the air inlet pipe (4).

9. The integrated living cell workstation of claim 8, wherein: cloth air chamber (6) are assembled by cultivateing room (3) lateral wall and separating net (61) and are constituteed, separate and install rotatable house steward (7) between net (61) central authorities and the cultivation room (3) lateral wall, house steward (7) lateral wall intercommunication has a plurality of branch pipes (71), the venthole has been seted up in branch pipe (71), house steward (7) cup joint formula intercommunication intake pipe (4).

10. An integrated living cell workstation according to claim 9, wherein: cultivate room (6) and install drive house steward (7) pivoted motor (8), house steward (7) cup joint fluted disc (72), the output of motor (8) have with fluted disc (72) meshing driven gear (81).