CN214004660U - Cell culture box with adjustable positive and negative pressure - Google Patents
Cell culture box with adjustable positive and negative pressure Download PDFInfo
- Publication number
- CN214004660U CN214004660U CN202022539967.0U CN202022539967U CN214004660U CN 214004660 U CN214004660 U CN 214004660U CN 202022539967 U CN202022539967 U CN 202022539967U CN 214004660 U CN214004660 U CN 214004660U
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- Prior art keywords
- pressure
- electromagnetic valve
- carbon dioxide
- box
- sensor
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- 238000004113 cell culture Methods 0.000 title claims abstract description 20
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 62
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 31
- 239000007789 gas Substances 0.000 claims abstract description 29
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 24
- 239000001301 oxygen Substances 0.000 claims abstract description 24
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 24
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229910052757 nitrogen Inorganic materials 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 230000010261 cell growth Effects 0.000 abstract description 8
- 230000012010 growth Effects 0.000 abstract description 8
- 230000008859 change Effects 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 4
- 238000000338 in vitro Methods 0.000 abstract description 3
- 230000004060 metabolic process Effects 0.000 abstract description 3
- 238000004088 simulation Methods 0.000 abstract description 3
- 229910001873 dinitrogen Inorganic materials 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 14
- 230000001276 controlling effect Effects 0.000 description 7
- 238000010586 diagram Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 206010021143 Hypoxia Diseases 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 210000002449 bone cell Anatomy 0.000 description 1
- 230000037182 bone density Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell culture box with adjustable positive and negative pressure, which comprises a normal pressure box, a shelf, a programmable controller, an oxygen control electromagnetic valve, a carbon dioxide control electromagnetic valve, a gas pipeline, a pressure adjusting electromagnetic valve, a high-pressure carbon dioxide gas cylinder and a high-pressure nitrogen gas cylinder; and a positive air pump, a negative air pump, a carbon dioxide concentration sensor, an oxygen concentration sensor and a pressure sensor are arranged in the pressure box. The utility model can directly simulate the cell growth state and metabolism change under different pressure conditions, thereby satisfying the simulation of all conditions of cell growth; the utility model discloses both can simulate the pressurized state cell growth in vitro, also can study the growth state of cell under the high-altitude low-pressure condition. Therefore, the functions which cannot be independently realized by other machines can be realized by using the local machine; the influence of continuous pressure change, namely 'high or low' on the cells in the growth process can be simulated.
Description
Technical Field
The utility model belongs to the technical field of cell culture, in particular to adjustable type cell culture case of positive negative pressure.
Background
The cell culture box (carbon dioxide incubator) is mainly used for controlling the concentration of CO2, temperature, humidity and oxygen concentration in the cell culture process, and products of the type are widely applied to cell laboratories.
However, the cell culture boxes on the market at present simulate the in vitro growth process of cells under normal pressure, but cannot truly and effectively simulate the cell growth process of cells under the condition of pressure in vivo, for example, the cells at different parts of a human body are subjected to different pressures, the human body is at different altitudes, and the atmospheric pressure is different; the pressure environment to which the cells are subjected is increased when the underwater vehicle is submerged; when walking and other exercises, the pressure on the cells changes constantly, and the like, and the real human body environment cannot be simulated by a conventional cell culture box, and the phenomenon that the osteoporosis of the human body is relieved in the exercise state cannot be completely explained.
The currently searched related product is only 201811201257.8, namely a pressure control type cell hypoxia culture box, but the patent relates to the situation of negative pressure and can not apply pressure to cells.
Only by truly and effectively simulating the growth state of cells in a human body can the growth, metabolism and action mechanism of the cells in the human body be completely explained.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a simple and effective positive and negative pressure adjustability cell culture case to the problem that exists in current conventional cell culture case (can't really simulate the real growth state of in vivo environmental cell promptly) to each condition that is used for the internal cell growth of system simulation, including temperature, humidity, oxygen concentration, pressure situation etc..
The utility model aims at adopting the following technical means to realize: a positive and negative pressure adjustable cell culture box comprises an atmospheric box, a pressure box, a shelf, a programmable controller, an oxygen control electromagnetic valve, a carbon dioxide control electromagnetic valve, a gas pipeline, a pressure adjusting electromagnetic valve, a high-pressure carbon dioxide gas cylinder and a high-pressure nitrogen cylinder, wherein the high-pressure carbon dioxide gas cylinder and the high-pressure nitrogen cylinder are respectively connected with the carbon dioxide control electromagnetic valve and the oxygen control electromagnetic valve, and the gas pipelines connected with the high-pressure carbon dioxide gas cylinder and the high-pressure nitrogen cylinder are respectively connected with the atmospheric box and the pressure box after being converged; the atmospheric pressure box is separated from the pressure box through a shelf, and a temperature sensor, a humidity sensor, a heating module, a carbon dioxide concentration sensor and an oxygen concentration sensor are arranged in the atmospheric pressure box; a positive air pump, a negative air pump, a carbon dioxide concentration sensor, an oxygen concentration sensor and a pressure sensor are arranged in the pressure box; the pressure regulating electromagnetic valve is connected with the positive and negative air pumps, and the programmable controller is respectively connected with the oxygen control electromagnetic valve, the carbon dioxide control electromagnetic valve, the pressure regulating electromagnetic valve, the heating module, the temperature sensor, the humidity sensor, the carbon dioxide concentration sensor, the oxygen concentration sensor and the pressure sensor.
Preferably, a gas mixing and homogenizing box is arranged in the pressure box and connected with a gas pipeline.
Preferably, the gas cylinder further comprises a pressure circulation solenoid valve which is connected between the pressure tank and the gas cylinder.
Has the advantages that:
1. the method can directly simulate the change conditions of cell growth state and metabolism under different pressure conditions (other conditions are consistent), thereby meeting the simulation of all conditions of cell growth, such as the pressure change of bone cells in exercise movement can increase bone density and the like.
2. The conventional cell culture box can not control the pressure condition, and particularly, the positive pressure and the negative pressure can not be realized on one machine. The utility model discloses both can simulate the pressurized state cell growth in vitro, also can study the growth state of cell under the high-altitude low-pressure condition. Therefore, the functions which cannot be realized independently by other machines can be realized by the local machine.
3. The influence of continuous pressure change, namely 'high or low' on the cells in the growth process can be simulated.
Drawings
The present invention will be described in further detail with reference to the accompanying drawings.
FIG. 1 is a schematic structural diagram of the cell culture box with adjustable positive and negative pressures.
Fig. 2 is a control circuit diagram of the present invention.
Fig. 3 is a structural view of the case.
In the figure, 1-flowmeter I, 2-air inlet electromagnetic valve, 3-gas pipeline, 4-atmospheric pressure tank, 5-pressure tank, 6-flowmeter II, 7-pressure circulation electromagnetic valve, 8-positive and negative pressure pump, 9-pressure regulation electromagnetic valve, 10-gas mixing box, 11-sensor, 12-shelf, 13-heating unit.
Detailed Description
As shown in fig. 1-3, the utility model discloses a cell culture box with adjustable positive and negative pressure:
1. the equipment mainly comprises: conventional cell incubators, programmable controllers, positive and negative air pumps, solenoid valves, relays, linking lines, other accessories such as CO2 sensors, oxygen sensors, pressure sensors, etc. The functions of each part are as follows:
1.1 conventional cell culture incubator: and providing proper temperature, humidity and CO2, namely controlling the temperature and the humidity and also performing parallel comparison.
1.2 positive and negative air pumps: positive pressure and negative pressure can be provided by positive and negative rotation.
1.3 programmable controller: adjusting various parameters and controls of the positive and negative control pressure adjustable cell culture system, such as controlling the concentration of CO2, the concentration of oxygen, positive pressure and negative pressure, controlling various relays, controlling the switch of an electromagnetic valve and the like.
1.3 electromagnetic valve: the gas flow path is controlled to be opened or closed, and the gas is aerated or closed.
1.4 sensor: measuring CO2 concentration, oxygen concentration, pressure, etc
1.5 air flow meter: controlling gas flow rate, etc., and placing air for rapid regulation
The working principle of the device is as follows: the computer is programmed and connected with the programmable controller, the computer inputs the required pressure, oxygen concentration and carbon dioxide concentration and operates, and the programmable controller compares the set value with the measured value of the sensor after receiving the instruction.
2.1 if the oxygen concentration is not enough, giving an instruction through the controller, opening an oxygen control valve and inputting oxygen; if the oxygen concentration is too high, the controller instructs to open the exhaust valve to exhaust the mixed gas, and simultaneously open the nitrogen control valve to input nitrogen.
2.2 if the CO2 concentration is not sufficient or too high, CO2 gas is fed or the mixture gas is removed through a solenoid valve.
2.3 if the pressure is not enough, giving out an instruction through a programmable controller, and adjusting the pressure of an air pump, an air bottle and the like.
And continuously detecting the signals of more than 2.4, and feeding back to the controller, so as to adjust to proper pressure and concentration ranges and maintain the same.
For traditional carbon dioxide incubator, three gas incubators even, the utility model discloses on this basis, malleation, negative pressure controlling means have been increased to through the required relevant parameter of computer input, can satisfy. Wherein, the pressure range can reach-30 KPa to +30KPa, and later stage through upgrading the structure of air pump and box, the pressure adjustment range scope can be more extensive.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing examples, or some features may be substituted. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (3)
1. The utility model provides an adjustable type cell culture case of positive negative pressure which characterized in that: the device comprises a normal pressure box, a shelf, a programmable controller, an oxygen control electromagnetic valve, a carbon dioxide control electromagnetic valve, a gas pipeline, a pressure regulating electromagnetic valve, a high-pressure carbon dioxide gas cylinder and a high-pressure nitrogen cylinder, wherein the high-pressure carbon dioxide gas cylinder and the high-pressure nitrogen cylinder are respectively connected with the carbon dioxide control electromagnetic valve and the oxygen control electromagnetic valve, and the gas pipelines connected with the high-pressure carbon dioxide gas cylinder and the high-pressure nitrogen cylinder are respectively connected with the normal pressure box and the pressure box after being converged; the atmospheric pressure box is separated from the pressure box through a shelf, and a temperature sensor, a humidity sensor, a heating module, a carbon dioxide concentration sensor and an oxygen concentration sensor are arranged in the atmospheric pressure box; a positive air pump, a negative air pump, a carbon dioxide concentration sensor, an oxygen concentration sensor and a pressure sensor are arranged in the pressure box; the pressure regulating electromagnetic valve is connected with the positive and negative air pumps, and the programmable controller is respectively connected with the oxygen control electromagnetic valve, the carbon dioxide control electromagnetic valve, the pressure regulating electromagnetic valve, the heating module, the temperature sensor, the humidity sensor, the carbon dioxide concentration sensor, the oxygen concentration sensor and the pressure sensor.
2. The positive-negative pressure-adjustable cell culture chamber according to claim 1, wherein: the pressure tank is internally provided with a gas mixing and equalizing box which is connected with a gas pipeline.
3. The positive-negative pressure-adjustable cell culture chamber according to claim 1, wherein: the gas cylinder pressure control device further comprises a pressure circulation electromagnetic valve, and the pressure circulation electromagnetic valve is connected between the pressure box and the gas cylinder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022539967.0U CN214004660U (en) | 2020-11-05 | 2020-11-05 | Cell culture box with adjustable positive and negative pressure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022539967.0U CN214004660U (en) | 2020-11-05 | 2020-11-05 | Cell culture box with adjustable positive and negative pressure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214004660U true CN214004660U (en) | 2021-08-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022539967.0U Expired - Fee Related CN214004660U (en) | 2020-11-05 | 2020-11-05 | Cell culture box with adjustable positive and negative pressure |
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| Country | Link |
|---|---|
| CN (1) | CN214004660U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023232099A1 (en) * | 2022-06-02 | 2023-12-07 | 北京中赢谷投资管理有限公司 | Method for obtaining vesicles |
-
2020
- 2020-11-05 CN CN202022539967.0U patent/CN214004660U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023232099A1 (en) * | 2022-06-02 | 2023-12-07 | 北京中赢谷投资管理有限公司 | Method for obtaining vesicles |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210820 |
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| CF01 | Termination of patent right due to non-payment of annual fee |