CN104745472B - A kind of plant tissue cultivating pot - Google Patents
A kind of plant tissue cultivating pot Download PDFInfo
- Publication number
- CN104745472B CN104745472B CN201510173239.3A CN201510173239A CN104745472B CN 104745472 B CN104745472 B CN 104745472B CN 201510173239 A CN201510173239 A CN 201510173239A CN 104745472 B CN104745472 B CN 104745472B
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- China
- Prior art keywords
- liquid
- circulating device
- circulation pipe
- plant tissue
- liquid outlet
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/02—Percolation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Abstract
The invention discloses a kind of plant tissue cultivating pot, it includes：Culture tank, tank external circulating device, pneumatic circulating device, gas distributor；Tank external circulating device includes:Add liquid mixer, culture liquid filter；Pneumatic circulating device includes：Culture tank connecting portion, air driven mixer；Described air driven mixer is divided into upper chamber, middle part narrow, lower cavity, and lower cavity side is provided with circulation pipe inlet；Tank external circulating device can nutrient solution filtered, solve addition nutrient solution when, mix it is uneven the problem of, nutrition can be added in real time.Pneumatic circulating device does not increase shearing force, realizes compressed air and nutrient solution gas-liquid mixed, bubble is small, uniform, while also further being mixed to annex solution and nutrient solution, mixed effect is more preferable using compressed air is passed through for power.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of plant tissue cultivating pot.
Background technology
China is one of resources of medicinal plant most abundant country in the world, and a large amount of with autonomic drug use, medicinal to plant
The excavation of thing and using the speed of growth considerably beyond plant, resources of medicinal plant is increasingly exhausted.Traditional medicinal plant is big
On the one hand field cultivation is influenceed by " favourable climate and geographical position ", and output and quality fluctuation is big, and another aspect pesticide residual contamination problem is serious.Plant
The bioreactor culture of thing cell, tissue and organ has growth rapid, it is easy to the characteristics of controlling, thus can gradually replace
The need for field production meets market.
Bioreactor is the reactor for large-scale culture microorganism, plant and animal cell, and plant is cultivated at present
The bioreactor of cell, tissue and organ has arrived the stage of industrialized production.For example, using airlift bioreactor
Cultivate Catharanthus Roseus Cell production serfin [A.H.Scragg et al., Enzyme Microb Technol, 1987,9：619]
；South Korea S.H.Son et al. carries out reactor large-scale culture to yew cell, using the gas-lifting type from 100L to 500L
Reactor [S.H.Son et al., Plant Cell Reports (2000) 19：628-633] ；After several years, Kee-Yoeup
Paek carries out large-scale culture to ginseng adventitious root using spherical bubbling style reactor and obtains the ginseng with very strong pharmacological activity
Saponin(e [Kee-Yoeup Paek et al., Adv Biochem Engin/Biotechnol (2009) 113：151-176], obtain
Obtained compared with ginseng adventitious root of height increament etc..
The reactor cultivated that suspended currently used for Medicinal Plant Cell, tissue and organ mainly has stirring-type, gas-lifting type, drum
Bubble etc..Since the 80's of eighties of last century, the culture of plant cell, tissue and organ is with reactor just via stirring-type
To gas-lifting type and bubble type transition.Because plant cell has volume larger, cell membrane is more crisp and with spies such as big vacuoles
Point, plant cell is very sensitive to shearing force, and its sensitiveness is by the species of cell, state, shearforce tolerance, shear rate and anti-
The many factors such as the structure of device in itself are answered to determine.For this feature, the approach of solution is typically from cell line, sieve
Select the high yield system of anti-shearing force or existing cell line is transformed by cell engineering and genetic engineering means, set up anti-shearing
The strain of power；Structure of reactor is transformed on this basis, makes it have the flow field of mitigation and good mixed performance.
Stirring type bioreactor opereating specification is big, and oxygen delivery capacity is strong, and what is produced in good mixing effect, but whipping process cuts
Shear force is larger, and damage is easily caused to Vitro Plant tissue, and energy consumption is larger.Comparatively, airlift reactor and bubble type
Reactor shearing force is smaller, and it is the widely applicable bioreactor of a class to generally believe bubbling style reactor both at home and abroad at present, this
Class reactor is structurally characterized in that bubble rises along liquid phase, contacted with liquid phase reacted while agitation liquid to improve biography
Matter speed.
The body of bioreactor uses ball-crown body structure and conical structure, overcomes cylindrical gas-lifting type biological respinse
Device and Sparged bioreactors easily produce the shortcoming of bubble, so as to avoid plant cell by the volatility thing in bubble
The influence of matter carbon dioxide etc gas.
To meet feed supplement is typically provided with the nutritional need of the whole incubation of plant tissue, the body of bioreactor
Mouth, thief hatch, periodically or non-periodically sample during culture, are supplemented nutrient solution, contained with determining dry cell weight, saponin(e
The Expenditure Levels of amount, polyoses content and carbon source, nitrogen source and phosphorus source.So as to adjust nutrient solution.To find optimal nutrient solution.
Initial incubation liquid is added due to disposable in incubation, it is contemplated that the consumption in growth course, initial incubation liquid is excessive.
This excessive nutrient solution, can influence the growth of earlier plant tissue, and the plant tissue of early stage is fragile, the requirement to nutrient solution
Also it is just further harsh.
Sometimes it is also required to add Cucumber in specific culture period, to improve containing for active drug composition in culture
Amount.
Because existing bubbling style reactor shearing force is smaller, mix uneven, Organization individual growth is uneven, while also influenceing
Yield and product quality.
The content of the invention
The purpose of the present invention is larger to solve stirring reactor shearing force, and easily Vitro Plant tissue is caused to damage
Wound, and bubbling style reactor shearing force is smaller, the problem of mixing uneven, and a kind of plant tissue cultivating pot is provided.
A kind of plant tissue cultivating pot, it includes：Culture tank 1, tank external circulating device 2, the compressed air mouth of pipe 4, gas point
Cloth device 5, exhaust apparatus；
Described tank external circulating device 2 includes:Annex solution pipe valve 21, addition liquid pipe 22, addition liquid mixer 23, circulation pipe
25th, cycle tube valve 27；
Described culture tank 1 is provided with liquid outlet 12 and circulation pipe inlet 323；
Described circulation pipe inlet 323 is located at the lower section of gas distributor 5, on liquid outlet 12 is located in the body of culture tank 1
Portion, by circulation pipe 25, is sequentially connected with addition liquid mixer 23, circulation pipe inlet 323；
Described addition liquid pipe 22 is connected on liquid feeding blender 23；
Described tank external circulating device 2 also includes culture liquid filter 24；
Described liquid outlet 12 is multiple, is symmetrical arranged, to ensure that pot liquid is steady；
Described liquid outlet 12 is additionally provided with liquid outlet screen pack 26；
Described culture tank 1 is provided with a groove 11, and liquid outlet 12 is located on the lower trough rim of groove 11, the openings of liquid outlet 12
To downward；
Addition liquid mixer 23 is a cavity, and adding tube liquid 22 is connected on addition liquid mixer 23；It is parallel with circulation pipe 25
Set；
Pneumatic circulating device 3 includes：Culture tank connecting portion 31, air driven mixer 32；32 points described of air driven mixer is
Upper chamber 321, middle part narrow 322, lower cavity 324, the side of lower cavity 324 are provided with circulation pipe inlet 323, bottom
Provided with the compressed air mouth of pipe 4, gas distributor 5 is arranged on above upper chamber, in culture tank connecting portion 31；Circulation pipe inlet
323 are connected with circulation pipe 25；
The described compressed air mouth of pipe 4 is in the top of circulation pipe inlet 323.
The invention provides a kind of plant tissue cultivating pot, it includes：Culture tank, tank external circulating device, pneumatic circulating dress
Put, gas distributor；Tank external circulating device includes:Add liquid mixer, culture liquid filter；Pneumatic circulating device includes：Training
Support tank connecting portion, air driven mixer；Described air driven mixer is divided into upper chamber, middle part narrow, lower cavity, lower chamber
Body side surface is provided with circulation pipe inlet；Can add nutrition in real time, tank external circulating device can nutrient solution filtered, solve
When adding nutrient solution, the problem of mixing uneven, pneumatic circulating device is power using the compressed air being passed through, and does not increase shearing
Power, realizes compressed air and nutrient solution gas-liquid mixed, and bubble is small, uniform, while also further to annex solution and nutrient solution
Mixing, mixed effect is more preferable.
Brief description of the drawings
Fig. 1 is a kind of overall structure figure of plant tissue cultivating pot of the invention；
Fig. 2 is a kind of partial sectional view of the tank external circulating device of plant tissue cultivating pot of the invention；
Fig. 3 is a kind of partial sectional view of the pneumatic circulating device of plant tissue cultivating pot of the invention.
Embodiment
Embodiment 1
Refer to Fig. 1-3, a kind of plant tissue cultivating pot, it includes：Culture tank 1, tank external circulating device 2, pneumatic circulating
Device 3, the compressed air mouth of pipe 4, gas distributor 5, exhaust apparatus；
Tank external circulating device 2 includes:Annex solution pipe valve 21, addition liquid pipe 22, addition liquid mixer 23, culture liquid filter
24th, circulation pipe 25, liquid outlet screen pack 26, cycle tube valve 27；
The described liquid outlet 12 of culture tank 1 is located in the middle part of the body of culture tank 1, by circulation pipe 25, with culture liquid filter
24 are sequentially connected with addition liquid mixer 23；
Described liquid outlet 12 is additionally provided with liquid outlet screen pack 26；
Addition liquid mixer 23 is a cavity, and adding tube liquid 22 is connected on addition liquid mixer 23；It is parallel with circulation pipe 25
Set；
Pneumatic circulating device 3 includes：Culture tank connecting portion 31, air driven mixer 32；32 points described of air driven mixer is
Upper chamber 321, middle part narrow 322, lower cavity 324, the side of lower cavity 324 are provided with circulation pipe inlet 323, bottom
Provided with the compressed air mouth of pipe 4, gas distributor 5 is arranged on above upper chamber, in culture tank connecting portion 31；Circulation pipe inlet
323 are connected with circulation pipe 25；
The described compressed air mouth of pipe 4 is in the top of circulation pipe inlet 323；
Described liquid outlet 12 is multiple, is symmetrical arranged, to ensure that pot liquid is steady；
Described culture tank 1 is provided with a groove 11, and liquid outlet 12 is located on the lower trough rim of groove 11, the openings of liquid outlet 12
To downward.
In use, adjustment nutrient solution is the nutrient solution for the plant tissue growth for being best suitable for primary stage of inoculation, addition liquid pipe is closed
Nutrient solution is added in valve 21, cycle tube valve 27, culture tank 1, inoculation opens air compressor, adjusts air pressure, at this moment cultivate
Tank is run by common bubble type culture tank, can be after plant tissue growth is stable, by each period nutrient consumption of plant tissue
Level calculation setting, control annex solution addition, open annex solution pipe valve 21, cycle tube valve 27, compressed air rises, pneumatic
In the presence of device, nutrient solution is filtered through liquid outlet screen pack 26, is entered culture liquid filter 24 from liquid outlet 12, is filtered laggard
Enter to add liquid mixer 23, annex solution enters after addition liquid mixer 23 mixes with nutrient solution from adding tube liquid 22, into pneumatic
EGR 3, gas-liquid mixed, gas distributor 5 redistribution gas-liquid, culture materials under gravity, to gas distributor 5
Concentrate, circulated under the drive of gas-liquid flow in nutrient solution；Nutrient solution is in the interior circulation of tank external circulating device 2 simultaneously.It can add in real time
Ensure Liquid composition.The present invention is without any power is added, using the power for the compressed air being passed through in Sparged bioreactors,
Nutrient solution is filtered using tank external circulating device, annex solution is mixed with nutrient solution, pneumatic circulating device not only provides power,
Gas-liquid mixed is also achieved, bubble is small, uniform, and secondary mix has been carried out with nutrient solution to annex solution.
Claims (7)
1. a kind of plant tissue cultivating pot, it includes：Culture tank（1）, gas distributor（5）, compressed air hose（4）, exhaust dress
Put, it is characterised in that it also includes：Tank external circulating device（2）；Described tank external circulating device（2）Including:Annex solution pipe valve
（21）, addition liquid pipe（22）, addition liquid mixer（23）, circulation pipe（25）, liquid outlet screen pack（26）, cycle tube valve（27）；
Described culture tank（1）Provided with liquid outlet（12）With circulation pipe inlet（323）；Described circulation pipe inlet（323）It is located at
Gas distributor（5）Lower section, liquid outlet（12）It is located at culture tank（1）Body middle and upper part, passes through circulation pipe（25）, it is mixed with annex solution
Clutch（23）, circulation pipe inlet（323）It is sequentially connected；Described addition liquid pipe（22）It is connected on addition liquid mixer（23）On；
It is additionally provided with pneumatic circulating device（3）, including：Culture tank connecting portion（31）, air driven mixer（32）；Described pneumatic mixing
Device（32）It is divided into upper chamber（321）, middle part narrow（322）, lower cavity（324）, lower cavity（324）Side is provided with and followed
Endless tube inlet（323）, bottom is provided with compressed air hose（4）, compressed air hose（4）Outlet in lower cavity（324）It is interior, follow
Endless tube inlet（323）Top, nearly middle part narrow（322）Place；Gas distributor（5）Above upper chamber, training
Support tank connecting portion（31）It is interior；Circulation pipe inlet（323）With circulation pipe（25）It is connected.
2. a kind of plant tissue cultivating pot according to claim 1, it is characterised in that：Described tank external circulating device（2）
Also include culture liquid filter（24）.
3. a kind of plant tissue cultivating pot according to claim 1, it is characterised in that：Described liquid outlet（12）To be many
It is individual, it is symmetrical arranged, to ensure that pot liquid is steady.
4. a kind of plant tissue cultivating pot according to claim 1,2 or 3, it is characterised in that：Described culture tank (1) is set
There is a groove (11), liquid outlet (12) is located on the lower trough rim of groove (11), and the opening direction of liquid outlet 12 is downward.
5. a kind of plant tissue cultivating pot according to claim 4, it is characterised in that：Described liquid outlet (12) is additionally provided with
Liquid outlet screen pack (26).
6. a kind of plant tissue cultivating pot according to claim 5, it is characterised in that：It is a chamber to add liquid mixer (23)
Body, adds liquid pipe（22）With circulation pipe（25）It is arranged in parallel.
7. a kind of plant tissue cultivating pot according to claim 6, it is characterised in that：Described compressed air hose（4）
Circulation pipe inlet（323）Top.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510173239.3A CN104745472B (en) | 2015-04-14 | 2015-04-14 | A kind of plant tissue cultivating pot |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510173239.3A CN104745472B (en) | 2015-04-14 | 2015-04-14 | A kind of plant tissue cultivating pot |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104745472A CN104745472A (en) | 2015-07-01 |
| CN104745472B true CN104745472B (en) | 2017-09-19 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510173239.3A Expired - Fee Related CN104745472B (en) | 2015-04-14 | 2015-04-14 | A kind of plant tissue cultivating pot |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10933347B2 (en) | 2017-08-18 | 2021-03-02 | Delta Separations, Llc | Apparatus and method for separating plant trichomes using a double vortex turbulence |
| CN112970569A (en) * | 2021-04-02 | 2021-06-18 | 长春工程学院 | Household type aerosol culture method for reducing nitrate of cherry radish |
| CN117256478B (en) * | 2023-11-22 | 2024-02-02 | 上海科缔联科技有限公司 | Continuous fermentation culture system for plant tissues |
Citations (2)
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|---|---|---|---|---|
| CN101130744A (en) * | 2007-07-26 | 2008-02-27 | 上海交通大学 | Airlift type bioreactor system for continuously bottling and culturing medicinal plant histiocyte |
| CN201102956Y (en) * | 2007-08-22 | 2008-08-20 | 上海日泰医药设备工程有限公司 | Outer circulation photosynthetic reaction bio-fermentation device |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| LU82145A1 (en) * | 1980-02-07 | 1981-09-10 | Cipari | IMPROVEMENTS IN PROCESSES AND PLANTS USING YEAST |
| US8501475B2 (en) * | 2011-12-21 | 2013-08-06 | Heliae Development Llc | Systems and methods for contaminant removal from a microalgae culture |
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2015
- 2015-04-14 CN CN201510173239.3A patent/CN104745472B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101130744A (en) * | 2007-07-26 | 2008-02-27 | 上海交通大学 | Airlift type bioreactor system for continuously bottling and culturing medicinal plant histiocyte |
| CN201102956Y (en) * | 2007-08-22 | 2008-08-20 | 上海日泰医药设备工程有限公司 | Outer circulation photosynthetic reaction bio-fermentation device |
Non-Patent Citations (2)
| Title |
|---|
| A perfusion air-lift bioreactor for high density plant cell cultivation and secreted protein production;Wei Wen Su等;《Journal of Biotechnology》;19961231(第50期);第225-233页 * |
| Development of a phenol degrading fluidized bed bioreactor for constant biomass holdup;A. G. Livingston等;《The Chemical Engineering Journal》;19911231(第45期);第B35-B47页 * |
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| CN104745472A (en) | 2015-07-01 |
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Granted publication date: 20170919 Termination date: 20210414 |