CN106434284A - Modular microalgae culture system with rapid algal species expanding culture device - Google Patents
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Abstract
A modular microalgae culture system with a rapid algal species expanding culture device comprises the algal species expanding culture device, a photobioreactor, a culture medium adding system, a gas delivery system and an algae solution delivery and supernate backflow system, wherein the algal species expanding culture device is connected with the culture medium adding system, the photobioreactor is respectively connected with the algal species expanding culture device, the culture medium adding system, the gas delivery system and the algae solution delivery and supernate backflow system and is inclined, and the algal species expanding culture device is installed on the backlight side of the photobioreactor. The system is in modular design and convenient to install and adjust, all modules are manufactured in advance and assembled on site, substances among the modules are delivered through pipes in operation, installation, maintenance and management difficulty is greatly reduced, the number of element modules can be adjusted according to working conditions at any time, production is maintained under the optimal working condition, and microalgae yield can be greatly improved.
Description
Technical field
The present invention relates to a kind of culture systems for realizing the culture of microalgae large scale and high density high yield.Belong to microalgae life
Thing field of engineering technology.
Background technology
Microalgae is that a kind of growth is rapid and the extremely strong biology of environmental suitability, various biochemical substances contained by its living beings, such as
Grease, protein, carbohydrate and pigment etc. are respectively provided with higher economic worth, great DEVELOPMENT PROSPECT.Particularly in the last few years,
Microalgae is considered as producing one of raw material of future of bio-fuel.
Carry out the Main Patterns that photoautotrophy culture is microdisk electrode in bioreactor.Carrying out large-scale culture
When, need a large amount of algae kinds.Traditionally, the production of algae kind be microalgae under the conditions of photoautotrophy, realized using the mode of spreading cultivation step by step
, exist the cycle of spreading cultivation oversize and be easy to because pollution lead to spread cultivation unsuccessfully, the low problem of cell density that provides, become obstruction
The main bugbear of microalgae extensive photoautotrophy culture.Photoautotrophy culture is compared, and it is high, biological that Heterotrophic culture has growth rate
Matter yield is high and advantage that avoid the pollution of wild algae species.As increased a set of closed reactor in microalgae culture system,
Wherein heterotrophism spreads cultivation microalgae cell as algae kind, undoubtedly can be greatly lowered algae kind spread cultivation failure risk, shorten and spread cultivation the cycle
And improve cell density in Photoreactor, thus the yield of microalgae is greatly improved.
The weather that the outside scenery of microalgae is faced and environmental condition fluctuation are larger, and actual operating mode is changeable, need to train
Foster system is frequently adjusted to each unit.
Therefore, it is necessary to provide a kind of modularization microalgae culture system of the device that quickly spreads cultivation with algae kind, to reduce microalgae
The difficulty that mass production system arranges, safeguards and adjust, and microalgae biomass is greatly improved, the particularly yield of grease.
Content of the invention
It is an object of the invention to the shortcoming overcoming conventional microdisk electrode reactor to exist, provide a kind of mounting and adjusting side
Just, the modularization microalgae culture system of the device that quickly spreads cultivation with algae kind of microalgae yield can be increased substantially.
The present invention quickly spreads cultivation with algae kind the modularization microalgae culture system of device, takes technical scheme below：
This system, spreads cultivation device, bioreactor, culture medium add-on system, gas delivery system and algae solution including algae kind
Conveying and five modules of supernatant return-flow system；Bioreactor is obliquely installed, and the algae kind device that spreads cultivation is installed on Photoreactor
Backlight side；
Described algae kind spreads cultivation device, including the device cavity that spreads cultivation, be provided between the upper and lower in cavity middle water conservancy diversion every
Plate, cavity two ends are respectively arranged with the culture medium input port connecting with cavity internal upper part, and the top in cavity is provided with agitator,
The bottom of cavity is provided with algae seedling solution delivery outlet, and algae seedling solution delivery outlet is connected with flow controller, algae seedling solution delivery outlet and light
Algae seedling solution input port on bioreactor connects, and algae seedling solution delivery pipe is connected with metering peristaltic pump；
Described bioreactor, including reactor cavity, is provided with diaphragm in reactor cavity body, by reactor cavity
Body is divided into the left and right sides communicating up and down, and left side is provided with aeration orifice plate, and aeration orifice plate is evenly distributed with pore, left bottom
It is provided with air inlet；Bottom right side is provided with algae seedling solution input port and backflow supernatant and culture medium import；Right side is provided with algae
Liquid delivery outlet, algae solution delivery outlet is connected with algae solution delivery pipe；The top of reactor cavity is provided with exhaust outlet；
Described culture medium add-on system, including nutrient storage tank, organic matter storage tank and culture medium storage tank, nutrient storage tank,
The culture medium input port that organic matter storage tank and culture medium storage tank are spread cultivation on device by feed pipe and algae kind respectively is connected, and culture medium stores up
Tank is connected with the backflow supernatant on bioreactor and culture medium import by feed pipe, all feed pipes is all connected with count
Amount peristaltic pump；
Described gas delivery system, including CO2Gas cylinder, air compressor, gas mixer and air shooter, CO2Gas cylinder
All with gas flowmeter, gas flowmeter is all connected with gas mixer with air compressor, gas mixer and gas
Delivery pipe connects, and is connected with gas mass flow controller, on air shooter and bioreactor in air shooter
Air inlet connect；
Described algae solution conveying and supernatant return-flow system, including solid-liquid separator, algae solution delivery pipe and supernatant return duct,
Algae solution delivery pipe is connected with solid-liquid separator, and one end of supernatant return duct is connected with solid-liquid separator, the other end and photo-biological
The backflow supernatant of reactor and culture medium import connect.
Described bioreactor is rack-mount, carries angle adjuster between bioreactor and support.
Described bioreactor is 50 ° -70 ° with horizontal plane angle.
The algae seedling solution delivery outlet that described algae kind spreads cultivation in device is towards Photoreactor side.
Algae solution equipped at outlet port in described reactor cavity body is provided with algae solution drainage plate.
Described reactor cavity internal upper part is provided with liquid level sensor, and to prevent, liquid level is too high to be revealed by exhaust outlet.
The present invention can be used for microalgae large-scale culture and the production of related microalgae bioenergy；Can cultivate can Heterotrophic culture
And grow rapid microalgae, such as chlorella, Scenedesmus quadricauda, scenedesmus obliquus, chrysophyceae, Phaeodactylum tricornutum, diatom etc.；There is following spy
Point：
1. adopt modularized design, all modules all manufacture in advance and produce, and assemble at the scene, are fixed with support, during operation
Material between each module is all conveyed by pipe fitting, substantially reduces installation, maintenance and management difficulty, and can be adjusted according to operating mode at any time
Whole each unit component count, production is maintained under optimum operating condition.
2. each intermodule is connected with pipe fitting, and the relative position free degree is big, can make full use of place space.
3. spread cultivation device using independent heterotrophism algae kind, run with bioreactor simultaneously, algae kind is greatly lowered and spreads cultivation
The risk of failure, shorten the cycle that spreads cultivation and the cell density that improves in Photoreactor is to 8-10g L-1, biomass yield can be carried
High 4-6 times.
4. it is respectively adopted heterotrophism/richness nitrogen and autotrophy/nitrogen stress culture in algae kind spreads cultivation device with bioreactor, significantly carry
High fat content, during culture chlorella, lipid-producing is 10 times about of general bioreactor.
5. utilize aerator, so that algae solution is circulated in the reactor, good algae solution mixing, supplement can be played inorganic
Carbon source and the effect removing satiety conjunction oxygen, prevent frustule adherent growth, mitigate the poison that supersaturation oxygen produces to frustule
Evil.
6. the most of supernatant after microalgae harvesting is back in reactor, saves the use of water and all kinds of chemical reagent
Amount, reduces the discharge of waste water；Inside reactor fills liquid level sensor, and liquid level can be prevented from exhaust outlet leakage too high.
7 measuring pumps and gas flowmeter all can be controlled using micro computer, both can achieve and manually control, and also can achieve automation
Control.
Brief description
Fig. 1 is the planar structure schematic diagram of the present invention,
Fig. 2 is the side schematic view of the present invention.
Fig. 3 is the structural representation of bioreactor in the present invention,
Fig. 4 is that in the present invention, algae kind spreads cultivation the structural representation of device.
Fig. 5 is that in the present invention, algae kind spreads cultivation the side sectional view of device.
In figure：1. algae solution delivery pipe；2. supernatant return duct；3. medium feed pipe；4. bioreactor；5. cultivate
Base transfer pipeline；6. nitrate solution feed pipe；7. glycerite feed pipe；8. algae kind spreads cultivation device；9. algae seedling solution delivery pipe；
10. nitrate solution storage tank；11. glycerite storage tanks；12. culture medium storage tanks；13. supports；14.CO2Gas cylinder；15. gases mix
Clutch；16. air compressors；17. gas transfer pipelines；18. reactor cavities；19. exhaust outlets；20. liquid level sensors；21.
Diaphragm；22. algae solution drainage plates；23. algae solution delivery outlets；24. algae seedling solution input ports；25. backflow supernatants and culture medium enter
Mouthful；26. aeration orifice plates；27. air inlets；28. culture medium input ports；29. spread cultivation device cavity；30. agitators；31. middle water conservancy diversion
Dividing plate；32. algae seedling solutions (the cell algae solution of kind containing algae) delivery outlet.
Specific embodiment
As depicted in figs. 1 and 2, the modularization microdisk electrode of the present invention.Including algae kind spread cultivation device 8, bioreactor 4,
Culture medium add-on system, gas delivery system and algae solution conveying and five modules of supernatant return-flow system.Bioreactor 4 is pacified
It is contained on support 13, the angled adjuster of support 13 upper band, for adjusting the angle of inclination of bioreactor 4.Photo-biological is anti-
Device 4 is answered to be 50-70 ° with horizontal plane angle.The algae kind device 8 that spreads cultivation is installed on the backlight side of Photoreactor 4.All modules are all made in advance
Make production, assemble at the scene, during operation, the material between each module is all conveyed by pipe fitting.Can Heterotrophic culture and biological rapidly
Microalgae all can be cultivated in the present invention, such as chlorella, Scenedesmus quadricauda, scenedesmus obliquus, chrysophyceae, Phaeodactylum tricornutum, diatom etc., can use
Production work in microalgae large-scale culture and related microalgae bioenergy.
Algae kind spread cultivation device 8 structure as shown in Figure 4 and Figure 5, including the device cavity 29 that spreads cultivation, the upper and lower in cavity 29
Between be provided with middle diaphragm 31.Cavity 29 two ends are respectively arranged with the culture medium input port connecting with cavity internal upper part
28, the top in cavity 29 is provided with multigroup agitator 30 being intervally arranged.It is defeated that the bottom of cavity 29 is provided with multiple algae seedling solutions
Outlet 32, algae seedling solution delivery outlet 32 is connected with flow controller.Algae seedling solution delivery outlet 32 towards Photoreactor 4 side, one
Algae seedling solution delivery outlet 32 is connected with a Photoreactor 4, and algae seedling solution delivery outlet 32 passes through algae seedling solution delivery pipe 9 and optical-biological reaction
Algae seedling solution input port 24 on device 4 connects (referring to Fig. 1), and algae seedling solution delivery pipe 9 is connected with metering peristaltic pump.
The structure of bioreactor 4, as shown in figure 3, including reactor cavity 18, is printing opacity lucite material, has
Preferably performance.Middle setting in cavity 18 has diaphragm 21, and cavity 18 is divided into the left side communicating up and down and right side two
Part, left side is provided with the aeration orifice plate 26 in raised arch, and aeration orifice plate 26 is evenly distributed with pore.Aeration orifice plate 26 with
There is cavity between cavity 18.The left bottom of cavity 18 is provided with air inlet 27.Cavity 18 bottom right side is provided with algae seedling solution
Input port 24 and backflow supernatant and culture medium import 25.Backflow supernatant and culture medium import 25 respectively with supernatant return duct
2 and medium feed pipe 3 connect (referring to Fig. 1), on medium feed pipe 3 connect metering peristaltic pump.The right side setting of cavity 18
There is algae solution delivery outlet 23, at the algae solution delivery outlet 23 in cavity 18, be provided with algae solution drainage plate 22.Algae solution delivery outlet 23 with treat
Harvest algae solution delivery pipe 1 and connect (referring to Fig. 1).The top of cavity 18 is provided with exhaust outlet 19, exhaust outlet 19 and aeration orifice plate 26
Tiltedly right.Cavity 18 internal upper part is provided with liquid level sensor 20, and to prevent, liquid level is too high to be revealed by exhaust outlet.
Culture medium add-on system, including nitrate solution storage tank 10, glycerite storage tank 11 and culture medium storage tank 12, referring to
Fig. 1.The culture medium that nitrate solution storage tank 10 is spread cultivation on device 8 with algae kind by nitrate solution feed pipe 6 and metering peristaltic pump
Input port 28 connects, the training that glycerite storage tank 11 is spread cultivation on device 8 with algae kind by glycerite feed pipe 7 and metering peristaltic pump
Foster base input port 28 connects.The training that culture medium storage tank 12 is spread cultivation on device 8 with algae kind by culture medium delivery pipe 5 and metering peristaltic pump
Foster base input port 28 connects.Nitrate solution, glycerite and culture medium are mixed into algae kind and spread cultivation in device 8.Meanwhile, culture medium
Storage tank 12 is connected with the backflow supernatant on bioreactor 4 and culture medium import 25 by medium feed pipe 3.Nitrate
(generally sodium nitrate) solution) as nutrient.Glycerite is input to algae kind as organic matter and spreads cultivation in device 8, input quantity by
Metering peristaltic pump controls.
Gas delivery system includes CO2Gas cylinder 14, air compressor 16, gas mixer 15 and air shooter 17, ginseng
See Fig. 1.CO2All with gas flowmeter, CO on gas cylinder 14 and air compressor 162Gas on gas cylinder 14 and air compressor 16
Flowmeter body is all connected with gas mixer 15, and gas mixer 15 is connected with air shooter 17.Connect in air shooter 17
It is connected to gas mass flow controller, air shooter 17 is connected with the air inlet 27 of bioreactor 4.With air pump or gas
The CO of bottle conveying2, sterile air or CO2It is passed through bioreactor 4 with sterile air mixed gas as source of the gas.
Algae solution conveying and supernatant return-flow system, including solid-liquid separator, algae solution delivery pipe 1 and supernatant return duct 2, scheme
In be not drawn into solid-liquid separator.Algae solution delivery pipe 1 is connected with solid-liquid separator, and algae solution is delivered to solid-liquid separating equipment.Supernatant
One end of liquid return duct 2 is connected with solid-liquid separator, the backflow supernatant of the other end and bioreactor 4 and culture medium import
25 connections, most of supernatant is back in bioreactor 4.
A set of modularization microalgae culture system can arrange one group of algae kind spread cultivation device 8,1-2 group culture medium add-on system, one group
Gas delivery system and 1-10 group bioreactor 4, one group of algae solution conveying of every group of bioreactor 4 configuration and supernatant return
Streaming system and one group of mounting bracket.The particular number of assembly and installation site are flexibly controlled by field condition and production requirement.
Algae kind spreads cultivation one of main modular that device 8 is microalgae culture system.The biology that the device cavity 29 that spreads cultivation is bred for algae kind
Reaction compartment.It is mixed with nitrate solution and the culture medium of glycerite enters, by culture medium input port 28, the device cavity 29 that spreads cultivation
Interior, mix with original algae seedling solution at once in the stirring of agitator 30, and in the device that spreads cultivation under the guiding of middle diaphragm 31
Constantly circulate in cavity 29, and constantly breed；Part algae seedling solution is derived as algae kind by algae seedling solution delivery outlet 32, by algae
Plant liquid delivery pipe 9 and metering peristaltic pump conveying enters bioreactor 4 by algae seedling solution input port 24.Heterotrophism is utilized in the present invention
The algae kind of condition spread cultivation device for bioreactor provide algae kind, be greatly lowered algae kind spread cultivation failure risk, shorten spread cultivation
Cycle simultaneously improves cell density in Photoreactor, thus the yield of microalgae is greatly improved.
Bioreactor 4 is one of main modular of microalgae culture system.Cavity in reactor cavity 18 is microalgae
The biological respinse space of growth.Algae seedling solution enters reactor cavity 18, backflow supernatant and culture medium from algae seedling solution input port 24
Entered in reactor cavity 24 by backflow supernatant and culture medium import 8, mix with original algae solution at once.Mixed gas are from air inlet
Mouth 27 enters in reactor cavities 18, forms tiny bubble through aeration orifice plate 26, carries out aeration and stirring to algae solution, and in water conservancy diversion
The lower formation of dividing plate 21 guiding circulates, and the gas rising to liquid level is discharged through blast pipe 19.Anti- by liquid level sensor 20
Feedback controls air inlet feed liquor speed.It is partly recirculated to the algae solution of non-aeration side under algae solution drainage plate 22 guiding by algae solution delivery outlet 23
Discharge through algae solution delivery pipe 1, as algae solution to be harvested.Replace quartzy aeration head using aeration orifice plate, aeration can be effectively improved
The uniformity, increase the utilization rate to gas for the microalgae, reduce the interference to micro algae growth for the turbulent flow, thus improving micro algae growth effect
Rate, thus improving the microalgae concentration in reactor, reduces algae adherent growth.Setting diaphragm 21, on the one hand improves anti-
Answer the mechanical strength of device, on the one hand under the cooperation of unilateral aeration, form algae solution circulating in reactor, be conducive to algae
Cell contact with gas and nutriment, increases mass-transfer efficiency and the utilization ratio of raw material.Using liquid level sensor 20
Feedback information automatically controls air inlet feed liquor speed, stablizes liquid level, prevents the unfavorable operations such as liquid flooding.
In work, CO2CO in gas cylinder 142With the air of air compressor 16 output, mix in proportion through gas flowmeter
Mixed by gas mixer 15 afterwards, input bioreactor 4 through air shooter 17.It is due-in that bioreactor 4 exports
Obtain algae solution and sent to by algae solution delivery pipe 1 and carry out separation of solid and liquid, the supernatant major part after separation of solid and liquid is back to optical-biological reaction
In device 4.
Claims (6)
1. a kind of modularization microalgae culture system of the device that quickly spreads cultivation with algae kind, is characterized in that, spreads cultivation device, photoproduction including algae kind
Thing reactor, culture medium add-on system, gas delivery system and algae solution conveying and five modules of supernatant return-flow system；Photo-biological
Reactor is obliquely installed, and the algae kind device that spreads cultivation is installed on the backlight side of Photoreactor；
Described algae kind spreads cultivation device, including the device cavity that spreads cultivation, is provided with middle diaphragm, chamber between the upper and lower in cavity
Body two ends are respectively arranged with the culture medium input port connecting with cavity internal upper part, and the top in cavity is provided with agitator, cavity
Bottom be provided with algae seedling solution delivery outlet, algae seedling solution delivery outlet is connected with flow controller, algae seedling solution delivery outlet and photo-biological
Algae seedling solution input port on reactor connects, and algae seedling solution delivery pipe is connected with metering peristaltic pump；
Described bioreactor, including reactor cavity, is provided with diaphragm in reactor cavity body, reactor cavity is divided
For the left and right sides communicating up and down, left side is provided with aeration orifice plate, aeration orifice plate is evenly distributed with pore, left bottom setting
There is air inlet；Bottom right side is provided with algae seedling solution input port and backflow supernatant and culture medium import；It is defeated that right side is provided with algae solution
Outlet, algae solution delivery outlet is connected with algae solution delivery pipe；The top of reactor cavity is provided with exhaust outlet；
Described culture medium add-on system, including nutrient storage tank, organic matter storage tank and culture medium storage tank, nutrient storage tank, organic
The culture medium input port that thing storage tank and culture medium storage tank are spread cultivation on device by feed pipe and algae kind respectively is connected, and culture medium storage tank leads to
Cross feed pipe to be connected with the backflow supernatant on bioreactor and culture medium import, all feed pipes are all connected with measure compacted
Dynamic pump；
Described gas delivery system, including CO2Gas cylinder, air compressor, gas mixer and air shooter, CO2Gas cylinder and sky
All with gas flowmeter on air compressor, gas flowmeter is all connected with gas mixer, and gas mixer is conveyed with gas
Pipe connects, and is connected with gas mass flow controller in air shooter, on air shooter and bioreactor enter
Gas port connects；
Described algae solution conveying and supernatant return-flow system, including solid-liquid separator, algae solution delivery pipe and supernatant return duct, algae solution
Delivery pipe is connected with solid-liquid separator, and one end of supernatant return duct is connected with solid-liquid separator, the other end and optical-biological reaction
The backflow supernatant of device and culture medium import connect.
2. the modularization microalgae culture system of the device that quickly spreads cultivation with algae kind according to claim 1, is characterized in that, described
Bioreactor is rack-mount, carries angle adjuster between bioreactor and support.
3. the modularization microalgae culture system of the device that quickly spreads cultivation with algae kind according to claim 1, is characterized in that, described
Bioreactor and horizontal plane angle are 50-70.
4. the modularization microalgae culture system of the device that quickly spreads cultivation with algae kind according to claim 1, is characterized in that, described
The algae seedling solution delivery outlet that algae kind spreads cultivation in device is towards Photoreactor side.
5. the modularization microalgae culture system of the device that quickly spreads cultivation with algae kind according to claim 1, is characterized in that, described
Algae solution equipped at outlet port in reactor cavity body is provided with algae solution drainage plate.
6. the modularization microalgae culture system of the device that quickly spreads cultivation with algae kind according to claim 1, is characterized in that, described
Reactor cavity internal upper part is provided with liquid level sensor.
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| CN107142197A (en) * | 2017-06-23 | 2017-09-08 | 四川省天惠能源科技有限公司 | A kind of biogas microalgae joint ecological treatment system and its application |
| WO2020132760A1 (en) * | 2018-12-28 | 2020-07-02 | Universidad Andrés Bello | Inclined culture system and method for spore resuspension for the spore culture of red algae |
| CN111943360A (en) * | 2020-07-27 | 2020-11-17 | 邵盛熙 | Prevention and treatment method for algal bloom disasters of eutrophic water body |
| CN113755325A (en) * | 2021-08-31 | 2021-12-07 | 河海大学 | Microalgae culturing and separating device based on built-in light source of N/S-CQDs |
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