CN106281989A - Laboratory combination type tray solid-state fermentation installation - Google Patents

Laboratory combination type tray solid-state fermentation installation Download PDF

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Publication number
CN106281989A
CN106281989A CN201610879827.3A CN201610879827A CN106281989A CN 106281989 A CN106281989 A CN 106281989A CN 201610879827 A CN201610879827 A CN 201610879827A CN 106281989 A CN106281989 A CN 106281989A
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CN
China
Prior art keywords
box body
flexible pipe
combination type
state fermentation
spraying hose
Prior art date
Application number
CN201610879827.3A
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Chinese (zh)
Inventor
常帆
贾凤安
吕睿
上官亦卿
丁浩
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陕西省微生物研究所
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Priority to CN201610879827.3A priority Critical patent/CN106281989A/en
Publication of CN106281989A publication Critical patent/CN106281989A/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/16Solid state fermenters, e.g. for koji production
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/22Transparent or translucent parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02Filters

Abstract

The present invention relates to a kind of laboratory combination type tray solid-state fermentation installation, this device includes the transparent cartridge of a band top cover, the left side of described box body and right upper portion all offer the air exchange hole that multiple level is contour, cover and arrange aseptic filtration core at the air exchange hole of box body;The left side of box body or the bottom on right side are offered one and are collected flexible pipe perforation, wear collection flexible pipe in collecting flexible pipe perforation, and box body is by collecting the external dismountable receiving flask of flexible pipe;Offering a spraying hose perforation in the middle part of the front side or rear side of box body, wear spraying hose in spraying hose perforation, spraying hose one terminates atomizer, and atomizer is fixedly installed on top cover lower surface, another termination water replanishing device of spraying hose;Offer multiple preformed hole in the front side of box body or upper back, in different preformed holes, be respectively provided with temperature probe, moisture probe or PH probe.The present invention test room fermentation condition not under easily contaminate miscellaneous bacteria, it is adaptable to carry out high-throughout fermentation research.

Description

Laboratory combination type tray solid-state fermentation installation

Technical field

The invention belongs to biological fermentation equipment technical field, be specifically related to a kind of laboratory combination type tray solid fermentation dress Put.

Background technology

Solid fermentation is that microorganism is not having or substantially do not having the fermentation mode on the solid state substrate of free water, solid state substrate Middle gas, liquid, solid three-phase is also deposited, containing water and water-insoluble materials in the most porous solid state substrate.Solid fermentation has in China The long history of thousand of years, for functional food, biological medicine, bio-feritlizer, the fermenting and producing of numerous products such as enzyme preparation.Tool There is production efficiency high, small investment, the advantages such as fermented product enzyme system enriches, environmentally safe.Solid fermentation is typically by not dissolving in The substrate of water, without or with a small amount of Free water.

Up to now, the most eurypalynous solid-state fermentation reactor comes out.Then can be divided into the motion conditions of substrate Two classes: 1. static solid-state fermentation reactor, including tray formula and post type reactor;2. dynamic solid-state fermentation reactor, including Churned mechanically letter, hydrant and rotating drum type reactor etc..Really be used for laboratory research scale seldom has approved product.

Tray formula reactor, tray formula bioreactor (Tray Bioreactor) tray formula reactor, belong to static anti- Answer device, operate relatively easy, use flexibly, tray number can be increased.The temperature and humidity of tray indoor is easily controlled.But it is this Owing to there is convected air in reactor, radiating effect is poor, has limited the thickness of tray material, but for laboratory level Conduct a research the most suitable.

Preferably laboratory scale solid fermentation bioreactor has following characteristics: (1) must be hard for the material built Gu, entirety can high-temperature heat sterilization (121 DEG C), light be easily moved and combination stacked, the microorganism to sweat is nontoxic. (2) visualized, the operating parameter such as sweat temperature, humidity, pH can monitor at any time, and fermentation condition is easily controllable and adjusts Whole.(3) entrance of sweat pollutant, the organism simultaneously controlling sweat generation is prevented to be discharged into environment.Because it is big Most solid ferment process contains fungal spore, it may be possible to pathogenic, the human and environment of surrounding can be produced potential hazard. (4) less energy consumption, easy and simple to handle, in the hope of cost-effective and manpower.

Traditional experiment room scale solid fermentation all uses open shallow tray fermentation or shaking flask solid fermentation.Open tray is sent out Ferment fermentation materials is exposed in open environment, easily causes the fungal contamination in air, and cannot ferment in the most same incubator Different strain and material;Sweat moisturizing difficulty and water content heterogeneity, sweat is difficult to control to.Shaking flask solid fermentation is empty Flow of air is poor, and sweat cannot monitor the parameters such as temperature, pH, humidity, and moisturizing process is loaded down with trivial details.

Summary of the invention

It is an object of the invention to provide a kind of laboratory combination type tray solid-state fermentation installation, solve solid-state of the prior art and send out Ferment device easily contaminates miscellaneous bacteria under testing room fermentation condition, it is impossible to the shortcoming carrying out high flux fermentation research.

In order to solve the problems referred to above, the technical scheme is that

Laboratory combination type tray solid-state fermentation installation, is positioned over outdoor or blowing-type microbiological incubator or electrical heating declines life In thing incubator, this device includes that the transparent cartridge of a band top cover, the left side of described box body and right upper portion are all offered multiple The air exchange hole that level is contour, covers at the air exchange hole of box body and arranges aseptic filtration core;The left side of box body or the right side The bottom of side is offered one and is collected flexible pipe perforation, wears collection flexible pipe in collecting flexible pipe perforation, and box body is external by collecting flexible pipe Dismountable receiving flask;In the middle part of the front side or rear side of box body, offer a spraying hose perforation, wear in spraying hose perforation If spraying hose, spraying hose one terminates atomizer, and atomizer is fixedly installed on top cover lower surface, the spraying hose other end Connect water replanishing device;Offer multiple preformed hole in the front side of box body or upper back, in different preformed holes, be respectively provided with temperature Probe, moisture probe or PH probe.

Described box body is rectangular shape, cylindrical shape or Elliptic Cylinder shape.

Described atomizer is single spraying atomizer, flat raft many atomizations shower nozzle or distributing atomizer.

Described water replanishing device is needle injector or electric water pump.

Described air exchange hole is polygon, oval or circular, and in air exchange hole, plug sets aseptic filtration core.

Described receiving flask is sealed plastic bottle or sealed glass jars.

The size of described box body is mm × 160,430mm × 300 mm.

Beneficial effects of the present invention:

1. the present invention is exclusively at the special purpose device designed by experiment desk research solid fermentation;Device is the most semiclosed Formula, installs aseptic filtration core additional in air exchange hole, it is ensured that fermentation is omnidistance aseptic;Fog-spray nozzle direct inoculation can be passed through in transparent cartridge Pure culture microorganism or add sterilized water, it is achieved first two effect of same spray, also simplify apparatus structure;Device bottom sets waste liquid Bottle, makes the finished product of material fermentation and waste liquid all close recovery, does not pollute external environment;

2. the present invention is in addition to the temperature & relative humidity indicator being fixedly installed, and is reserved with the installing hole installing other probes additional, can ferment Journey is monitored more process data, provides convenient for laboratory research;

A kind of typical sizes of transparent cartridge the most of the present invention is mm × 160,430mm × 300 mm, and multiple transparent cartridge can be with heap It is stacked in the incubator of laboratory cultivation, it is not necessary to independent water power;Each transparent cartridge can individually ferment a kind of material, with Under the fermentation condition of sample, can ferment different materials simultaneously, saves time and the manpower of research.

Accompanying drawing explanation

Fig. 1 is the overall structure schematic diagram of the present invention;

In figure, 1-top cover, 2-box body, 3-air exchange hole, 4-aseptic filtration core, 5-collects flexible pipe, 6-receiving flask, and 7-spraying is soft Pipe, 8-atomizer, 9-water replanishing device, 10-preformed hole.

Detailed description of the invention

Below in conjunction with the accompanying drawings the present invention is further detailed, solid with reference to the laboratory combination type tray shown in Fig. 1 State installation for fermenting, is positioned in blowing-type microbiological incubator or electric heating type microbiological incubator, and this device includes a band The transparent cartridge 2 of top cover 1, the left side of described box body 2 and right upper portion all offer five contour air exchange holes 3 of level, Cover at the air exchange hole 3 of box body 2 and aseptic filtration core 4 is set;A collection is offered in the left side of box body or the bottom on right side Flexible pipe is bored a hole, and wears collection flexible pipe 5 in collecting flexible pipe perforation, and box body 2 is by collecting the external dismountable receiving flask 6 of flexible pipe 5;? Offer a spraying hose perforation in the middle part of the front side of box body 2 or rear side, wear spraying hose 7 in spraying hose perforation, spraying Flexible pipe 7 one terminates atomizer 8, and atomizer 8 is fixedly installed on top cover 1 lower surface, another termination water replanishing device of spraying hose 7 9;Offer multiple preformed hole 10 in the front side of box body 2 or upper back, in different preformed holes, be respectively provided with temperature probe, wet Degree probe or PH probe.

Described box body 2 is rectangular shape, cylindrical shape or Elliptic Cylinder shape.

Described atomizer 8 is single spraying atomizer, flat raft many atomizations shower nozzle or distributing atomizer.

Described water replanishing device 9 is needle injector or electric water pump.

Described air exchange hole 3 is polygon, oval or circular, and in air exchange hole 3, plug sets aseptic filtration core 4.

Described receiving flask 6 is sealed plastic bottle or sealed glass jars.

The size of described box body 2 is mm × 160,430mm × 300 mm.

The work process of the present invention be described below:

Open top cover 1, the tray bottom transparent cartridge 2 adds the substrate needing fermentation and strain to be studied, by fermented product Add to 3 ~ 5cm thick, be closing and sealing off top cover 1;

Being moved to by transparent cartridge 2 in atmospheric environment or incubator, the air-blast device of air or incubator drives germ-free air flow to pass through Multiple air exchange holes 3 of the transparent cartridge left and right sides enter box body, it is achieved the air exchange of transparent cartridge 2 inner space;Mist Change shower nozzle 8 and in transparent cartridge, inject sterilized water by needle injector or electric water pump, keep fermentation system interior humidity, with Meet thalli growth to oxygen, temperature, the demand of humidity;Can be straight by temperature, pH or the moisture probe in box body 2 preformed hole 10 See the monitoring every key parameter of solid fermentation in real time, it is achieved fermentation is omnidistance controlled;

The box body 2 of the present invention can stack placement, and then carries out multiple solid fermentation substrate and fermented bacterium simultaneously in same training Support the co-fermentation in case or culture environment, improve solid fermentation Efficiency, save manpower and time.

Through experiment, use the present invention solid-state fermentation installation can be outstanding complete Chinese medicine dreg solid fermentation, The solid fermentation of Monas cuspurpureus Went and Cordyceps fungus feed additive production task.

Present disclosure is not limited to cited by embodiment, and those of ordinary skill in the art are by reading description of the invention And the conversion of any equivalence that technical solution of the present invention is taked, the claim being the present invention is contained.

Claims (7)

1. laboratory combination type tray solid-state fermentation installation, is positioned over outdoor or blowing-type microbiological incubator or electrical heating declines In bio-incubator, it is characterised in that: this device includes the transparent cartridge (2) of a band top cover (1), a left side for described box body (2) Side and right upper portion all offer the air exchange hole (3) that multiple level is contour, cover at air exchange hole (3) place of box body (2) Aseptic filtration core (4) is set;The left side of box body or the bottom on right side are offered one and are collected flexible pipe perforation, collect in flexible pipe perforation Wearing collection flexible pipe (5), box body (2) is by collecting flexible pipe (5) external dismountable receiving flask (6);In the front side of box body (2) or On rear side of person, a spraying hose perforation is offered at middle part, wears spraying hose (7), spraying hose (7) one end in spraying hose perforation Connecing atomizer (8), atomizer (8) is fixedly installed on top cover (1) lower surface, another termination water replanishing device of spraying hose (7) (9);Offer multiple preformed hole (10) in the front side of box body (2) or upper back, in different preformed holes, be respectively provided with temperature Probe, moisture probe or PH probe.
Laboratory combination type tray solid-state fermentation installation the most according to claim 1, it is characterised in that: described box body (2) it is rectangular shape, cylindrical shape or Elliptic Cylinder shape.
Laboratory combination type tray solid-state fermentation installation the most according to claim 1 and 2, it is characterised in that: described mist Changing shower nozzle (8) is single spraying atomizer, flat raft many atomizations shower nozzle or distributing atomizer.
Laboratory combination type tray solid-state fermentation installation the most according to claim 3, it is characterised in that: described moisturizing dress Putting (9) is needle injector or electric water pump.
Laboratory combination type tray solid-state fermentation installation the most according to claim 4, it is characterised in that: described air is handed over Changing hole (3) is polygon, oval or circular, and air exchange hole (3) Nei Sai sets aseptic filtration core (4).
Laboratory combination type tray solid-state fermentation installation the most according to claim 5, it is characterised in that: described receiving flask (6) it is sealed plastic bottle or sealed glass jars.
Laboratory combination type tray solid-state fermentation installation the most according to claim 6, it is characterised in that: described box body (2) Size be mm × 160,430mm × 300 mm.
CN201610879827.3A 2016-10-09 2016-10-09 Laboratory combination type tray solid-state fermentation installation CN106281989A (en)

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CN201610879827.3A CN106281989A (en) 2016-10-09 2016-10-09 Laboratory combination type tray solid-state fermentation installation

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Application Number Priority Date Filing Date Title
CN201610879827.3A CN106281989A (en) 2016-10-09 2016-10-09 Laboratory combination type tray solid-state fermentation installation

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006027990A1 (en) * 2006-06-14 2007-12-27 Riegler, Hansjörg Fermentation cabinet or fermentation chamber for the acceleration of fermenting process in bakery products, comprises closed treatment chamber, heating device for heating the treatment chamber and a superheated steam device
CN102443535A (en) * 2011-05-10 2012-05-09 中国科学院过程工程研究所 Solid fermentation bioreactor
CN202530079U (en) * 2012-04-28 2012-11-14 娄底市裕德科技有限公司 Multi-functional spray solid-state fermentation tank
CN203860421U (en) * 2014-05-19 2014-10-08 中国农业科学院兰州畜牧与兽药研究所 Fermentation box of solid-state fermentation protein feed
CN203904325U (en) * 2014-06-13 2014-10-29 山东青州云门酒业(集团)有限公司 Fermentation device for producing Maotai-flavor white spirit
CN204070340U (en) * 2014-09-18 2015-01-07 青海省三江畜产品交易市场有限责任公司 Preservation of vegetables case
CN204653177U (en) * 2015-06-04 2015-09-23 伏家强 A kind of breeding box
CN105062884A (en) * 2015-08-31 2015-11-18 东北大学 Enrichment and cultivation device for hydrogen-producing bacteria through photo-fermentation and using method thereof
CN206173336U (en) * 2016-10-09 2017-05-17 陕西省微生物研究所 Laboratory combination formula shallow dish solid state fermentation device

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006027990A1 (en) * 2006-06-14 2007-12-27 Riegler, Hansjörg Fermentation cabinet or fermentation chamber for the acceleration of fermenting process in bakery products, comprises closed treatment chamber, heating device for heating the treatment chamber and a superheated steam device
CN102443535A (en) * 2011-05-10 2012-05-09 中国科学院过程工程研究所 Solid fermentation bioreactor
CN202530079U (en) * 2012-04-28 2012-11-14 娄底市裕德科技有限公司 Multi-functional spray solid-state fermentation tank
CN203860421U (en) * 2014-05-19 2014-10-08 中国农业科学院兰州畜牧与兽药研究所 Fermentation box of solid-state fermentation protein feed
CN203904325U (en) * 2014-06-13 2014-10-29 山东青州云门酒业(集团)有限公司 Fermentation device for producing Maotai-flavor white spirit
CN204070340U (en) * 2014-09-18 2015-01-07 青海省三江畜产品交易市场有限责任公司 Preservation of vegetables case
CN204653177U (en) * 2015-06-04 2015-09-23 伏家强 A kind of breeding box
CN105062884A (en) * 2015-08-31 2015-11-18 东北大学 Enrichment and cultivation device for hydrogen-producing bacteria through photo-fermentation and using method thereof
CN206173336U (en) * 2016-10-09 2017-05-17 陕西省微生物研究所 Laboratory combination formula shallow dish solid state fermentation device

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Application publication date: 20170104