CN104328048B - Superficial growth formula photosynthetic microorganism culture plate and its system - Google Patents
Superficial growth formula photosynthetic microorganism culture plate and its system Download PDFInfo
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- CN104328048B CN104328048B CN201410598702.4A CN201410598702A CN104328048B CN 104328048 B CN104328048 B CN 104328048B CN 201410598702 A CN201410598702 A CN 201410598702A CN 104328048 B CN104328048 B CN 104328048B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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Abstract
The application is related to a kind of superficial growth formula photosynthetic microorganism culture plate，Including the first outside plate and the second outside plate that are stationary relative to one another setting，The core body being arranged between the first outside plate and the second outside plate，The first intermediate layer being arranged between the core body and first outside plate，And the second intermediate layer being arranged between the core body and second outside plate，Orthogonal first direction and second direction are defined on the basis of the outer surface place plane of first outside plate of culture plate，First intermediate layer and second intermediate layer have the liquid percent of pass of gradual change in a second direction，So that after the liquid that the core body absorbs penetrates through the first outside plate and the second outside plate again by first intermediate layer and the second intermediate layer、Reach the uniformity on the outer surface of whole first outside plate and the second outside plate of the amount of liquid on the outer surface of the first outside plate and the second outside plate.
Description
Technical field
Present invention relates generally to microorganism culturing field.Specifically, the application be related to a kind of microbial culture system with
And the modified model composite surface growth formula photosynthetic microorganism culture plate and system used in microbial culture system.
Background technology
It is unique with light or the microorganism lived of main energy sources that photosynthetic microorganism is a class, including microalgae, indigo plant
Antibacterial etc. can carry out photosynthetic microorganism containing chlorophyll, and microorganism has important biomolecule value.Particularly microalgae,
Rich in proteins, can be used as aquatic feed or animal and fowl fodder (such as spirulina)；Prior, some microalgae are under given conditions
Secondary metabolitess, such as oils and fatss, carotenoid, polysaccharide etc. can be synthesized in a large number, these materials are often with high economic worth
Bioactive substance, the fields such as functional food, food additive, pharmacy, bioenergy can be used in.Especially by micro-
Algae large-scale culture extracts microalgae grease, and then changes into liquid fuel, and such as biodiesel is considered as to solve bioenergy life
One of most important approach of product and carbon fixation and emission reduction.
The existing decades history of microalgae mass propgation, current industrialization microdisk electrode is liquid submersion, with a large amount of water
As the medium of micro algae growth.Mainly include open culture pond and Closed photobioreactor (photo-bioreactor,
PBR) two kinds of forms.The advantage of open culture pond is that the cost built and run is relatively low.But due to the plane of illumination in open pond
Product/volume is smaller, and liquid surface mixes poor with bottom, and only top layer frustule can receive more sufficient illumination, bottom of pond
Cell is often difficult to receive abundant illumination；Secondly, the depth of open pond training liquid is shallower, general only 10-30 centimetre so that logical
When gas mends carbon, gas-liquid contact time is short, and benefit carbon efficiencies are low, dissolved carbon dioxide (CO in culture fluid2) deficiency receive photosynthesis
To restriction, another is open intensification slowly, it is impossible to be raised to 25 DEG C best of temperature of enzymatic activity at short notice, Jing often misses light
The best opportunity for utilizing；And temperature declines too slow naturally in the Night Service formula pond, frustule is made to remain in that vigorous breathing
Effect, the energy expenditure that daytime stores is fallen, therefore makes metabolite content useful in frustule too low.Therefore open pond is cultivated
Vitro growth rates are relatively low with cell density, and which takes up an area also big in addition.In contrast, PBR-as be using light transmissive material (such as
Glass, lucite, plastic sheeting etc.) made by thin thin structure, as optical path is little, cultivating system illuminating area/volume ratio compared with
Greatly, so cell illumination is more abundant.Meanwhile, to mend carbon gas and liquid contact time is long, culture fluid concentration of ordinary dissolution is higher, thus carefully
Intracellular growth speed is high compared with open culturing pond with cell density.But such PBR generally involves great expense, operating cost high, it is tired to safeguard
It is difficult, be difficult to maximize, yield is in 5～30g/m2/ d, well below 100～200g/m of theory expectation2/ d, does not reach industrialization
Theoretical Calculation target, low this microalgae large-scale culture of the efficiency of light energy utilization realizes the most important direct restraining factors of industrialization still
Have much room for improvement.
In recent years, a kind of adhesion type culture systems are occurred in that, existing patent report, this adhesion type are using filter paper, filter
One or more in cloth, plastic foam, fabric (such as canvas) are used as host material, but such soft material possesses
Following shortcoming：1) mechanical strength is low, needs complicated support, the amplification in improper large scale and spatial altitude make
Short thin thin structure, and self-supporting is tended to, higher its water lock of porous material of rigidity is again than relatively limited, it is impossible to full well
Nutrition liquid measure needed for sufficient micro algae growth；2) surface moisture such as existing filter cloth and fabric is uneven, and micro algae growth is loosely
Gu；And 3) existing filter cloth and fabric etc. need Jing often to change intolerant to recycling, increase cost, 4) existing filter
The impurity such as the fiber that easily drops such as cloth and fabric；In addition, 5) these flexible materials when as culture plate surfacing, its
The aspect such as bio-compatibility and chemical resistance all has certain limitation.Therefore, such culture systems are not entered at present
Large-scale production.
For this purpose, the present inventor is in other application, a kind of microalgae culture system is proposed, which adopts the compound training of sandwich
Foster plate carries out the culture of microalgae.A kind of sandwich compound criteria plate 10 as shown in Figure 1a, which mainly includes two 12 Hes of outside plate
14 and the core body 16 between two outside plates 12 and 14.Wherein, outside plate is rigidity water suction dank material plate, and core body is suction
Water water penetration packing material.
In incubation, the core body 16 of sandwich compound criteria plate 10 absorbs culture fluid, in sandwich compound criteria plate
Inoculation attachment algae kind on the outer surface of 10 two outside plates 12 and 14, the culture fluid that core body 16 absorbs penetrate two 12 Hes of outside plate
14 and reach algae kind on the outer surface, in the case where appropriate ambient humidity and temperature, light intensity is arranged, algae is grown.It is above-mentioned
Sandwich compound criteria plate 10, by its sandwich composite structure, outside plate 12, the water yield on 14 surfaces are especially suitable for algae growth, and
Relative to training methods such as existing canvas, as its rigid material outside plate 12,14 can realize self-supporting easily, with space
Rate rate is high, resistance to environmental factorss are good, high recycling rate, will not fall the advantages such as fiber.
But, because there is certain altitude, such as 0.5-5.0 rice, in the core of culture plate in 10 in the vertical direction of culture plate
After body water suction saturation, unavoidably there is certain hydrostatic pressing in the vertical direction.As shown in Figure 1 b, as height H1 is less than height
Degree H2, the hydrostatic pressing at the B points at top edge height H2 is higher than the hydrostatic pressing at the A points at top edge height H1.
This phenomenon is caused, in the vertical direction, and the pressure of the water function of core body on outside plate is different, oozes through outside plate
The amount of the culture fluid for going out is different.It is, further away from the top edge of culture plate 10, i.e., the closer to the lower limb of culture plate 10, oozing
The culture fluid for going out outside plate 12 and 14 is more, or even a large amount of hydrops occurs, and the algae kind that outer plate surfaces are adhered to is washed away, nothing is caused
Method is normally cultivated.So, one piece of culture plate, a part of region can for algae apposition growth, it is a part of then be not suitable for algae life
Long, its utilization rate is reduced；Simultaneously as the Culture liquid measure that each region of one piece of culture plate outer surface is oozed out is different, each region is supported
The algae biomass that goes out, algae intracellular metabolite thing content are all presented larger difference, and this is certainly not intended to what is seen.
For this purpose, the present invention is desired to researching and developing a kind of superficial growth formula photosynthetic microorganism training for solving above-mentioned technical problem
Foster plate and system.
The content of the invention
It is an object of the invention to provide a kind of improved superficial growth formula photosynthetic microorganism culture plate so that penetrate into outside plate
Outer surface on culture fluid amount on whole outer surface uniformity.
For this purpose, according to an aspect of the invention, there is provided a kind of superficial growth formula photosynthetic microorganism culture plate, including phase
The first outside plate and the second outside plate to being fixed to one another setting, the core body being arranged between the first outside plate and the second outside plate are arranged at
The first intermediate layer between the core body and first outside plate, and be arranged between the core body and second outside plate
Second intermediate layer, is defined on the basis of the outer surface place plane of first outside plate of culture plate or the second outside plate orthogonal
First direction and second direction, first intermediate layer and second intermediate layer are with the liquid along the second direction gradual change
Percent of pass so that after the liquid that the core body absorbs penetrates through the first outside plate again by first intermediate layer, arrival the
Amount of liquid on the outer surface of one outside plate uniformity on the outer surface of whole first outside plate, and the liquid that the core body absorbs
Amount of liquid after body penetrates through the second outside plate again by second intermediate layer, on the outer surface of the second outside plate of arrival is whole
Uniformity on the outer surface of individual second outside plate.
According to an alternative embodiment, first intermediate layer and second intermediate layer have constant in the first direction
Liquid percent of pass.
According to an alternative embodiment, one or both of first intermediate layer and second intermediate layer be by liquid not
Plate body made by permeable material, and the plate body includes the part that liquid can pass through.
According to an alternative embodiment, the part that the liquid can pass through is the hole through the plate body.
According to an alternative embodiment, the plate body is included by the row extended along the first direction and along the second party
The array in the hole constituted to the row for extending.
According to an alternative embodiment, it is equally spaced apart along the first direction with the hole in a line evenly distributed.
According to an alternative embodiment, in the first end from the second direction towards to second in the second direction
End, the line space between adjacent lines have the trend for gradually increasing.
According to an alternative embodiment, the line space between the adjacent lines is increased with constant increment, or the increasing
Amount linearly changes.
According to an alternative embodiment, all holes in the array have identical diameter.
According to an alternative embodiment, in the first end from the second direction towards second in the second direction
On the direction at end, the diameter in each hole in same row is gradually reduced.
According to an alternative embodiment, the diameter in each hole in the same row is reduced with constant reduction amount, Huo Zhesuo
State reduction amount linearly to change.
According to an alternative embodiment, the plate body includes the array in the hole being made up of row and column, the row and described
One direction extends at an anglely, and the row extend at an anglely with the second direction.
According to an alternative embodiment, the hole have circular, square, rectangle, ellipse, triangle and they appoint
The cross section of meaning combined shaped.
According to an alternative embodiment, the hole includes the multiple elongated holes being arranged side by side along the first direction, respectively should
Elongated hole is extended along the second direction, and with the first end from the second direction towards in the second direction
The second end direction on gradually constriction width.
According to an alternative embodiment, in the hole filled with liquid permeable material or be provided with liquid can be saturating
The film crossed.
According to an alternative embodiment, one or both of first intermediate layer and second intermediate layer are configured to solid
The liquid permeable microporous membrane of the netted or sieve-like on first outside plate and second outside plate.
According to an alternative embodiment, the microporous membrane it is glued coating or spray or be printed onto first outside plate and
On the inner surface of the direction core body of one or both of second outside plate.
According to an alternative embodiment, the microporous membrane with first outside plate and the second outside plate identical chi
It is very little.
According to an alternative embodiment, the microporous membrane includes the multiple microporous membranes being arranged side by side along the second direction,
Each microporous membrane extends along the first direction, and wherein, in the first end from the second direction towards described second
On the direction at the second end on direction, the plurality of microporous membrane has the penetration by liquid rate being gradually reduced；Or
The microporous membrane includes the multiple microporous membranes being arranged side by side along the first direction, and each microporous membrane is along described second
Direction extend and with the first end from the second direction towards on the direction at the second end in the second direction
The penetration by liquid rate being gradually reduced.
According to an alternative embodiment, the superficial growth formula photosynthetic microorganism culture plate is vertically placed, the first party
To being horizontally oriented, and the second direction is vertical direction.
According to an alternative embodiment, the superficial growth formula photosynthetic microorganism culture plate is the culture for cultivating microalgae
Plate, the liquid are the culture fluid for microalgae.
According to a further aspect in the invention, there is provided a kind of including the micro- of above-mentioned superficial growth formula photosynthetic microorganism culture plate
Biological culture system.
The present invention superficial growth formula photosynthetic microorganism culture plate, by the water lock water-retaining property of its core body, can absorb and
Pin mass propgation liquid, and absorb saturation after, it is sustainable equably to outside the first outside plate of both sides and second seep water, by water,
The nutritional labelings such as inorganic salt are transported to outside plate outer surface, for growth of microorganism attached to it；Power is used for a long time need not
Equipment stirs and transports culture fluid, saves energy consumption；Relative to the culture of immersion, the microorganism on both sides outside plate of the present invention can be straight
Receive the irradiation of light source, optical energy loss amount can be reduced；Further, since microorganism such as algae, is directly affixed in solid phase,
Therefore growing environment can be changed easily, reduce artificial human cost.
Further effect is that the superficial growth formula photosynthetic microorganism culture plate of the present invention is photosynthetic in superficial growth formula
Microorganism culturing plate vertically place in the case of be it is very favorable, so just can effective room for promotion utilization rate.Intermediate layer
Setting can be alleviated and offset due to when culture plate is vertically placed, because of the problem of the hydrostatic pressure reduction brought by difference in height, so as to flat
Weighing apparatus culture fluid can avoid the lower area of the outer surface in outside plate from oozing out culture in the infiltration speed of each position of outer plate surfaces
Liquid is excessive, hydrops, causes culture fluid to waste, the microorganism that even water accumulating volume crosses senior general's attachment is washed away and departs from the technology of culture plate and ask
Topic, so as to improve the Biomass of culture plate unit area, is to realize that large-scale culture lays the foundation；And can reduce to outside plate
The technological requirement of requirement and the processing that may be needed with the material of core body itself.
Description of the drawings
Above and other feature and advantage of the present invention is by preferred embodiment mode below to being given with reference to the accompanying drawings
Description in be better understood, wherein：
Fig. 1 a illustrate that the sandwich composite surface of prior art grows the side view cutaway drawing of formula photosynthetic microorganism culture plate；
Fig. 1 b are the front views that the sandwich composite surface of Fig. 1 a grows formula photosynthetic microorganism culture plate；
Fig. 2 a show the diagrammatic side-view cross-sectional of the sandwich compound criteria plate with intermediate layer according to the application preferred embodiment
Figure；
Enlarged drawings of Fig. 2 b for the region Z in Fig. 2 a；With
Fig. 3 a to 3b are two kinds of exemplary distribution modes in the hole in intermediate layer；
Fig. 3 c show another optional embodiment in intermediate layer.
Specific embodiment
In this application, " microorganism " including but not limited to photosynthetic microorganism and plant cell, " microorganism culturing " includes
But the culture of photosynthetic microorganism and plant cell is not limited to, especially the culture of microalgae, and " liquid " i.e. training objective microorganism
Required culture fluid.
According to application, microbial culture system mainly includes culture liquid supply unit and sandwich composite surface growth formula light
Close microorganism culturing plate.It is that the sandwich with intermediate layer is combined according to the superficial growth formula photosynthetic microorganism culture plate of the application
Superficial growth formula photosynthetic microorganism culture plate, below with reference to the accompanying drawings describes in detail multiple according to the sandwich of the application preferred embodiment
Close superficial growth formula photosynthetic microorganism culture plate.
As illustrated in figures 2 a-2b, the training of formula photosynthetic microorganism is grown according to the sandwich composite surface of the application preferred embodiment
Foster plate reference 20 is represented.
Two outside plates 22 and 24 that superficial growth formula photosynthetic microorganism culture plate 20 includes being fixed relative to each other, between two
Core body 26 between individual outside plate 22, between core body 26 and outside plate 22 first intermediate layer 32, and be located at core body 26 and outside plate
Second intermediate layer 34 between 24.
Outside plate 22 or 24 is conventional rigidity in the art or the liquid permeable plate of material of flexibility, and has respectively
It is suitable to be inoculated with the outer surface 22a and 24a with cultivating microorganism.Core body 26 is conventional liquid permeable filling material in the art
Material.Intermediate layer 32 and 34 is made up of the material of liquid impermeability, and including being suitable to multiple holes 36 that water passes through (in Fig. 2 b
In illustrate).The example that can be used for the material in manufacture intermediate layer 32 and 34 includes but is not limited to polyethylene, PVC, PTFE etc..It is middle
Layer 32 and 34 can be configured to plate or film or other can realize the form of identical function.
In microbial cultivation process, culture fluid is provided to the core body of superficial growth formula photosynthetic microorganism culture plate 20
26, after core body 26 absorbs culture fluid saturation, the culture fluid in core body 26 reaches outside plate via the hole 36 on intermediate layer 32 and 34
22 and 24, outer surface 22a and 24a that outside plate 22 and 24 reaches outside plate 22 and 24 are then penetrated through, is outer surface 22a and 24a
On microorganism provide nutrition, realize microorganism culturing purpose.
In the case where superficial growth formula photosynthetic microorganism culture plate 20 is vertically placed, in order to be reduced or eliminated as far as possible by
The height of diverse location is different on the culture plate outer surface, the gravity of culture fluid and therefore different hydrostatic pressings of diverse location etc.
Impact to reaching the amount of the culture fluid on the outer surface 22a and 24a of outside plate 22 and 24, makes the whole of arrival outside plate 22 and 24
Culture fluid on individual outer surface 22a and 24a is more uniform, and the hole 36 in intermediate layer 32 and 34 vertically unevenly can be divided
Cloth.
Intermediate layer 32 is described below with reference to Fig. 3 a-3c.It may be noted that intermediate layer 32 and 34 preferably has identical shape
Shape, structure, size and arrangement, so the description to intermediate layer 32 is equally applicable to intermediate layer 34 below.
Fig. 3 a show first distribution pattern P1 in the hole 36 on intermediate layer 32.First distribution pattern P1 includes the m in hole 36
× n array.Specifically, first distribution pattern P1 in hole 36 includes multiple row R in hole 361-RmWith multiple row C1-Cn, i.e., per a line
RiIncluding n hole 36 and per string CjIncluding m hole 36, wherein, m is the integer more than 1, and n is the integer more than or equal to 1, and i is 1
Including the integer including end points and m between, j is including the integer including end points between 1 and n.The m row R in hole 36iIn intermediate layer
Vertically it is arranged side by side on 32, each row RiExtend along the horizontal direction perpendicular to vertical direction, the n row C in hole 36j
It is arranged side by side on intermediate layer 32 in the horizontal direction, each row CjVertically extend.
Each hole 36 in m n array has identical diameter.Each row RiIn each hole 36 in the horizontal direction each other etc.
It is spaced apart away from ground, the line space of adjacent lines is differing from each other.On direction from the top down, the line space between adjacent lines is gradually
Increase.According to practical situation, the variable quantity between adjacent line space is identical or different.Here it should be noted that, at this up and down
Wen Zhong, term " line space " is distance of the adjacent rows between two Ge Kongkong centers in same row, term " column pitch "
It is the distance between adjacent two row two Ge Kongkong centers in the same row.
In above-mentioned first distribution pattern P1, as the line space between adjacent rows gradually increases from the top down, can
The Culture liquid measure at least partially or fully offset on the outer surface that outside plate 32 and 34 is reached present in prior art is uneven
Technical problem.
It is different from the first distribution pattern P1 illustrated in 3a, in the second distribution pattern P2 illustrated in 3b, with a line Ri
In each hole there is identical diameter, same row CjIn diameter is sequentially reduced from the top down in each hole.Row between adjacent rows
Spacing is equal to each other, i.e., each row RiVertically it is spaced apart each other at equal intervals, the column pitch between adjacent two row is equal to each other,
I.e. each row CjIt is spaced apart each other at equal intervals in the horizontal direction.
In above-mentioned second distribution pattern P2, the hole 36 of m n array in intermediate layer 32 with whole line spaces it is all equal and
The all equal mode of whole column pitch equably arranges, but the hole with a line there is identical diameter and the hole of same row on to
Lower diameter is sequentially reduced.So, also can at least partially or fully can offset in prior art as the first distribution pattern P1
The uneven technical problem of Culture liquid measure on the outer surface of the arrival outside plate 32 and 34 of presence.
Those skilled in the art are appreciated that to preferably solve above-mentioned technical problem, it is contemplated that first
In distribution pattern P1, same row CjIn diameter is sequentially reduced from the top down in each hole；Or it is contemplated that in the second distribution pattern
In P2, the line space between adjacent rows gradually increases from the top down.
In addition, in the alternative embodiment of the first distribution pattern P1, the line space between adjacent rows from the top down can be with
Increased with constant increment, or increment can linear or other rules changes.In the same manner, in the optional reality of the second distribution pattern P1
Apply in example, same row CjIn each hole from the top down diameter variable quantity can with equal, or variable quantity can it is linear or other
Rule changes.
Those skilled in the art are further appreciated that row RiWith row CjIt is necessarily strict to extend along horizontally and vertically direction,
But, row RiWith row CjExtension is inclined at an angle can with horizontal or vertical direction respectively.
According to practical application and needs, the shape in hole 36 is not restricted to the circle for illustrating, rectangle, square, triangle, semicircle
The combination in any shape of shape, ellipse etc. and these geometries can be used.Hole 36 is also necessarily in vertical manner
Through intermediate layer 32, alternatively, hole 36 obliquely passes through intermediate layer 32.
Those skilled in the art can also be appreciated that the number in hole 36, one of number n of number m at once and row or two
Person, can select as needed.
In alternative embodiments, different from the hole 36 as shown in Fig. 3 a and 3b, intermediate layer 32 and 34 can include respectively
Along multiple elongated holes of horizontal Tile arrangement, each elongated hole vertically extends, and with gradually constriction from the top down
Width.
In other alternate embodiments, the permeable material of the Kong Zhongke liquid filling bodies in intermediate layer 32 and 34 or can set
Liquid permeable film is put, technical purpose same as described above is obtained.
Superincumbent description, according to intermediate layer 32 and 34 quilts of the superficial growth formula photosynthetic microorganism culture plate 20 of the application
Be configured to include fluid-impermeable part, such as hole 36, body by made by fluid non-permeate material plate body.Intermediate layer
32 and 34 can be fixed on outside plate 22 and 24 by any way, or can be provided only between core body 26 and outside plate 22 and 24 and not
It is fixed on outside plate 22 and 24 by any way.
However, applicant also envisages that intermediate layer 32 and 34 can be secured to the micro- of the netted or sieve-like on outside plate 22 and 24
Pore membrane.As shown in Figure 3 c, the fixation on intermediate layer 32 and 34 to outside plate 22 and 24 can by printing, coating, spraying, paste or
Other similar fashions are realized.
In the embodiment shown in Fig. 3 c, microporous membrane can be configured to k microporous membrane J being arranged side by side from the top downk, its
In, k is the integer more than 1.Strip of each microporous membrane into horizontal orientation, and with constant penetration by liquid rate, on to
Under the penetration by liquid rate of each microporous membrane that is arranged side by side be gradually reduced successively.
In unshowned another embodiment, microporous membrane can be configured to be arranged side by side from left to right mutually the same many
Individual microporous membrane.Each microporous membrane into vertically-oriented strip, and with the penetration by liquid rate being gradually reduced from the top down.
Those skilled in the art are also appreciated that in the case where can realize expectation function, and each microporous membrane can also be with
Vertically or horizontally angularly it is in tilted layout.
In unshowned another embodiment, the whole Zhang Wei that microporous membrane can be secured on the inner surface of outside plate 22
Pore membrane, which has a penetration by liquid rate of constant in the horizontal direction, in the vertical direction gradual change from the top down, penetration by liquid rate from
On be gradually reduced downwards.
According to the superficial growth formula photosynthetic microorganism culture plate of the application, including the first outside plate for being stationary relative to one another setting
With the second outside plate, the core body being arranged between the first outside plate and the second outside plate, be arranged at the core body and first outside plate it
Between the first intermediate layer, and the second intermediate layer being arranged between the core body and second outside plate, if with the culture plate
Orthogonal first direction and second direction are defined on the basis of the outer surface place plane of the first outside plate or the second outside plate, then
First intermediate layer and second intermediate layer with the liquid percent of pass along the second direction gradual change, from there through gradually
Increase the infiltration resistance from culture plate first end to the second end so that the liquid that the core body absorbs passes through first intermediate layer
After penetrating through the first outside plate again, the amount of liquid on the outer surface of the core body of the first outside plate is reached in whole appearance
Uniformity on face, and after the liquid of core body absorption penetrates through the second outside plate again by second intermediate layer,
Reach the amount of liquid on the outer surface of the core body of the second outside plate uniformity on whole outer surface.In superficial growth
In the case that formula photosynthetic microorganism culture plate is vertically placed, according to the superficial growth formula photosynthetic microorganism culture plate right and wrong of the application
Chang Youli's.
The setting in this intermediate layer, at least in significant fraction or can be completely counterbalanced by due on the vertical culture plate placed
The difference in height of diverse location, the gravity of culture fluid and the hydrostatic and caused outer surface for cultivating microorganism in outside plate
It is upper to obtain that culture fluid is uneven, and the technical problem that come off from plate of the bottom microorganism that thus results in so that
The microorganism cultivated on the outer surface of two outside plates is more consistent.
The setting in this intermediate layer, can avoid the bottom of the outer surface in outside plate especially bottom ooze out culture fluid it is excessive,
There is " sweating " phenomenon so as to cause the technical problem that culture fluid is wasted, make the amount of culture fluid needed for cultivating microorganism minimum,
Cost is efficiently controlled, and to realize that large-scale culture lays the foundation, additionally it is possible to reduce the material itself to outside plate and core body
Requirement and the processing that may be needed technological requirement.
Only describe the principle of the present invention above by way of example, but be not meant to limit the scope of the invention.Conversely,
Structure described herein or feature can be presented as many other forms, and the feature for describing in one embodiment can be with other
Further feature in embodiment combines and constitutes new embodiment.Without departing from the spirit and scope being defined by the claims
In the case of, those skilled in the art can carry out various alternatives and modifications to above-described embodiment.
Claims (21)
1. a kind of superficial growth formula photosynthetic microorganism culture plate, is characterized in that, including the first outside plate for being stationary relative to one another setting
With the second outside plate, the core body being arranged between the first outside plate and the second outside plate, be arranged at the core body and first outside plate it
Between the first intermediate layer, and the second intermediate layer being arranged between the core body and second outside plate is given birth on the surface
Long formula photosynthetic microorganism culture plate is located with the outer surface of first outside plate of culture plate or the second outside plate in the case of vertically placing
Orthogonal horizontal direction and vertical direction, first intermediate layer and second intermediate layer tool are defined on the basis of plane
There is the liquid percent of pass along the vertical direction gradual change so that the liquid that the core body absorbs is oozed again by first intermediate layer
It is thoroughly uniform on the outer surface of whole first outside plate by the amount of liquid after the first outside plate, on the outer surface of the first outside plate of arrival
Unanimously, and the core body absorb liquid the second outside plate is penetrated through again by second intermediate layer after, arrival second
Amount of liquid on the outer surface of outside plate uniformity on the outer surface of whole second outside plate.
2. superficial growth formula photosynthetic microorganism culture plate according to claim 1, is characterized in that, first intermediate layer and
Second intermediate layer has liquid percent of pass constant in the horizontal direction.
3. superficial growth formula photosynthetic microorganism culture plate according to claim 2, is characterized in that, first intermediate layer and
One or both of described second intermediate layer is the plate body by made by the material of liquid impermeable, and the plate body includes liquid
The part that can pass through.
4. superficial growth formula photosynthetic microorganism culture plate according to claim 3, is characterized in that, what the liquid can pass through
Part is the hole through the plate body.
5. superficial growth formula photosynthetic microorganism culture plate according to claim 4, is characterized in that, the plate body is included by edge
Row and the array in the hole constituted along the row that the vertical direction extends that the horizontal direction extends.
6. superficial growth formula photosynthetic microorganism culture plate according to claim 5, is characterized in that, with the hole in a line along institute
State horizontal direction be equally spaced apart it is evenly distributed.
7. superficial growth formula photosynthetic microorganism culture plate according to claim 6, is characterized in that, on the vertical direction
From the top down, the line space between adjacent lines has the trend for gradually increasing.
8. superficial growth formula photosynthetic microorganism culture plate according to claim 7, is characterized in that, between the adjacent lines
Line space is increased with constant increment, or the increment linearly changes.
9. superficial growth formula photosynthetic microorganism culture plate according to claim 8, is characterized in that, all in the array
Hole has identical diameter.
10., according to arbitrary described superficial growth formula photosynthetic microorganism culture plate in claim 5-8, it is characterized in that, described
On vertical direction from the top down, the diameter in each hole in same row is gradually reduced.
11. superficial growth formula photosynthetic microorganism culture plates according to claim 10, is characterized in that, in the same row
The diameter in each hole is reduced with constant reduction amount, or the reduction amount linearly changes.
12. superficial growth formula photosynthetic microorganism culture plates according to claim 4, is characterized in that, the plate body include by
The array in the hole that row and column is constituted, the row extend at an anglely with the horizontal direction, described to arrange and the vertical direction
Extend at an anglely.
13., according to arbitrary described superficial growth formula photosynthetic microorganism culture plate in claim 4-9, is characterized in that, the hole
Cross section with circular, square, rectangle, ellipse, triangle and their combination in any shape.
14. superficial growth formula photosynthetic microorganism culture plates according to claim 4, is characterized in that, the hole is included along institute
Multiple elongated holes that horizontal direction is arranged side by side are stated, each elongated hole is extended along the vertical direction, and with described vertical
The gradually width of constriction from the top down on direction.
15., according to arbitrary described superficial growth formula photosynthetic microorganism culture plate in claim 4-9, is characterized in that, described
Liquid permeable material is filled with hole or liquid permeable film is provided with.
16. superficial growth formula photosynthetic microorganism culture plates according to claim 3, is characterized in that, first intermediate layer
With one or both of second intermediate layer be configured to be fixed on first outside plate and second outside plate netted or
The liquid permeable microporous membrane of sieve-like.
17. superficial growth formula photosynthetic microorganism culture plates according to claim 16, is characterized in that, the microporous membrane is glued
Connect or coat or spray or be printed onto one or both of first outside plate and second outside plate the direction core body it is interior
On surface.
The 18. superficial growth formula photosynthetic microorganism culture plates according to claim 16 or 17, is characterized in that, the microporous membrane
With with first outside plate and the second outside plate identical size.
The 19. superficial growth formula photosynthetic microorganism culture plates according to claim 16 or 17, is characterized in that,
The microporous membrane includes the multiple microporous membranes being arranged side by side along the vertical direction, and each microporous membrane is along the horizontal direction
Extend, and wherein, the plurality of microporous membrane has the penetration by liquid being gradually reduced on the vertical direction from the top down
Rate；Or
The microporous membrane includes the multiple microporous membranes being arranged side by side along the horizontal direction, and each microporous membrane is along the vertical direction
Extend and with the penetration by liquid rate being gradually reduced on the vertical direction from the top down.
20., according to arbitrary described superficial growth formula photosynthetic microorganism culture plate in claim 1-9, is characterized in that, the table
Long formula photosynthetic microorganism culture plate of looking unfamiliar is the culture plate for cultivating microalgae, and the liquid is the culture fluid for microalgae.
21. a kind of microbial culture systems, including according to arbitrary described photosynthetic micro- life of superficial growth formula in claim 1-20
Thing culture plate.
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