CN2080522U - Vertical sheet cell incubator - Google Patents
Vertical sheet cell incubator Download PDFInfo
- Publication number
- CN2080522U CN2080522U CN 90226702 CN90226702U CN2080522U CN 2080522 U CN2080522 U CN 2080522U CN 90226702 CN90226702 CN 90226702 CN 90226702 U CN90226702 U CN 90226702U CN 2080522 U CN2080522 U CN 2080522U
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- China
- Prior art keywords
- plates
- cell culture
- vertical sheet
- container
- plate
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Abstract
The utility model discloses a vertical sheet cell culture device, which is a device for culturing cells in holes on a plate. The vertical sheet cell culture device is provided with a closed container which is filled with the water on the bottom, and the inner part of the vertical sheet cell culture device is provided with cell culture plates and moistening plates which are vertically arranged. The cell culture plates are provided with large numbers of holes without bottoms which are used for containing culture fluid; the cell culture plates are arranged between the moistening plates, and the surfaces of the moistening plates are coated with cloth which can suck the water from the bottom of the container. The container is arranged in an incubator with thermostatic circulating air. By means of the parallelization of a plurality of cell culture plates, and the vertical sheet cell culture device can contain a large quantity of liquid culture cells in a minor volume. After the inner part of the contain reaches the stable state, each point can have consistent temperature and saturated humidity. In the process of the operation, the dehydration in the holes is less, and the surfaces of the plates can not form the drop condensate water.
Description
The utility model relates to the device of culturing cell in a kind of hole onboard.
In Med Biol research, testing, widely used a kind of Tissue Culture Plate claims enzyme plate again, commonly used is the polystyrene board that 96 holes are arranged, and there is the end in the hole, is used to hold gobbet, the time spent horizontal positioned is carried out the liquid culture of cell, is furnished with lid on the plate.Such Tissue Culture Plate, the volume ratio tape casette holder is smaller, but can hold 96 and cultivate the unit, so very economical.But when requiring to cultivate element number and increase considerably, as 9600 unit, just need 100 blocks of this plates, at this moment will go wrong, at first be because their cumulative volumes are huge, cultivate, will cause each layer temperature inconsistent if overlay mutually in the common thermostat container layer by layer, next is that many like this culture plates are carried out various operations, and workload is very big.
The utility model purpose is the device that culturing cell in a kind of improved hole onboard will be provided, and it is compared with existing Tissue Culture Plate, in shared identical volume, can increase considerably and cultivate unitary quantity.
The purpose of this utility model is achieved in that the Tissue Culture Plate that will the no bottom outlet that hold nutrient solution be arranged assigns into two surfaces and keeps in the middle of the humidification plate of humidity, and these plates vertically are placed in the encloses container.Such arrangement can make the many intensive placements of Tissue Culture Plate, thereby compare with existing Tissue Culture Plate, and open ended cultivation unit number increases in the same volume.The temperature of these plates, humidity are identical after reaching steady state.Concerning same block of plate, reach steady state after, temperature, humidity are also identical up and down.Because container is airtight, reach steady state after, humidity is saturated, so moisture no longer reduces because of evaporation in the metapore.Container has been placed in the recirculating air homothermic incubator, when heat insulating culture, reduce the incubator heat-up rate, and make Tissue Culture Plate, humidification plate, container and incubator all begin to warm to the temperature that needs, can avoid being formed into the water of condensation that drips on the Tissue Culture Plate from same temperature.Little to a certain degree the time when the aperture, the no bottom outlet on the plate can hold nutrient solution and unlikely loss.Humidification plate surface is surrounded by from the cloth of container bottom suction, makes its surface keep moist always.
The utility model is because the intensive placement of many Tissue Culture Plates is more much more than the open ended cultivation unit number of 96 porocyte culture plates of existing traverse use in same volume.Square plastic plate of 22cm can hold 1500 in the hole of diameter 2mm, and the center in adjacent two holes is at a distance of Shang Keda 5mm.The cubes of a 22cm length of side can hold 15 blocks of such plates, has just comprised 22500 holes.In addition because the hole is the no end, when needing just so that they are carried out colorimetric analysis.This utility model successfully is used on the high-level efficiency screening installation of microbial mutation bacterial strain by us.
The concrete structure of utility model is provided by following embodiment and accompanying drawing thereof.
Fig. 1 is the sectional view according to the device of culturing cell in the hole on the plate that the utility model proposes.
Below in conjunction with Fig. 1, describe details in detail according to the concrete device that the utility model proposes.
This device comprises an encloses container (1), and Tissue Culture Plate (2) is arranged in the container, has or not bottom outlet (3) on the culture plate, and culture plate vertically is positioned between the axial humidification plate (4), and the cloth that is wrapping on humidification plate surface stretches in the water (5) of container bottom.
Claims (2)
1, the device of culturing cell in the hole onboard, this device has an encloses container (1), Tissue Culture Plate (2) and humidification plate (4) are arranged in the container, on Tissue Culture Plate (2), have or not bottom outlet (3), it is characterized in that humidification plate (4) vertically lays, Tissue Culture Plate (2) vertically is placed in the middle of the humidification plate (4).
2,, it is characterized in that there is the cloth that stretches into suction in container (1) bottom water (5) on humidification plate (4) surface according to the described device of claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90226702 CN2080522U (en) | 1990-12-24 | 1990-12-24 | Vertical sheet cell incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90226702 CN2080522U (en) | 1990-12-24 | 1990-12-24 | Vertical sheet cell incubator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2080522U true CN2080522U (en) | 1991-07-10 |
Family
ID=4903899
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 90226702 Pending CN2080522U (en) | 1990-12-24 | 1990-12-24 | Vertical sheet cell incubator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2080522U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1051575C (en) * | 1996-05-15 | 2000-04-19 | 中国科学院化工冶金研究所 | Reactor for nutrient liquid atomizing culture of plant |
| CN104328048A (en) * | 2014-10-30 | 2015-02-04 | 国家开发投资公司 | Surface growth type photosynthetic microorganism culture plate and surface growth type photosynthetic microorganism culture system |
-
1990
- 1990-12-24 CN CN 90226702 patent/CN2080522U/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1051575C (en) * | 1996-05-15 | 2000-04-19 | 中国科学院化工冶金研究所 | Reactor for nutrient liquid atomizing culture of plant |
| CN104328048A (en) * | 2014-10-30 | 2015-02-04 | 国家开发投资公司 | Surface growth type photosynthetic microorganism culture plate and surface growth type photosynthetic microorganism culture system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| AD01 | Patent right deemed abandoned | ||
| C20 | Patent right or utility model deemed to be abandoned or is abandoned |