CN107325969A - Pollen liquid sprouts device - Google Patents
Pollen liquid sprouts device Download PDFInfo
- Publication number
- CN107325969A CN107325969A CN201710783925.1A CN201710783925A CN107325969A CN 107325969 A CN107325969 A CN 107325969A CN 201710783925 A CN201710783925 A CN 201710783925A CN 107325969 A CN107325969 A CN 107325969A
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- Prior art keywords
- mounting table
- wet
- wet box
- box
- pollen
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention provides a kind of pollen liquid and sprouts device, including insulating box, wet box, mounting table, culture plate, and the wet box is placed in insulating box；Added with quantitative clear water in wet box；Mounting table is provided with wet box, culture plate is placed with mounting table, clear water can be added directly into wet box cassette bottom, wet box can also be divided into tank and non-irrigated groove two parts space, clear water is added in tank, mounting table is set in non-irrigated groove, if the wet box cassette bottom that clear water is added directly into, the both sides of wet gauze are hung down into wet box cassette bottom water；As clear water has been added in tank, the bottom that then mounting table in non-irrigated groove is set is in tank, its other end is placed in tank after wet gauze is placed on mounting table, mounting table can be separate part, can also be with the affixed one of wet box, the present invention for pollen liquid sprouting provides a kind of special equipment, it is simple in construction, ensure the suitable humiture environment that liquid is sprouted, pollen after sprouting can conveniently and be effectively collected after sprouting.
Description
Technical field
Device is sprouted the present invention relates to a kind of pollen liquid, particularly a kind of pollen liquid for scientific experiment sprouts dress
Put.
Background technology
The sexual reproduction of plant is always the hot issue of scientific research field, and the extension of pollen germination and pollen tube has been
Into the committed step of plant fertilization, therefore test, collected necessary to pollen after sprouting is scientific research in vitro.But
It is to be completed at present on the sprouting of pollen more on solid medium, the pollen after sprouting is collected less than seriously substantially
The research to plant fertilization process is hindered, therefore, in the urgent need to a kind of mode of Liquid Culture, the pollen after sprouting can lead to
The mode of filtering is collected.The factor of influence pollen germination is a lot, in addition to ionic environment, also temperature, humidity and oxygen, Gu
These conditions of body culture medium are relatively easy to control, and fluid nutrient medium, and because pollen germination required time is long, therefore condition is very
Hardly possible control, especially humidity, the space of incubator are larger, and the change of its humidity is also relatively rapider.In current invention, not on
Device in terms of pollen liquid sprouting.
The content of the invention
The technical problem to be solved in the present invention is that pollen liquid sprouts collection less than the pollen after sprouting in the prior art.
The present invention provides for solution above-mentioned technical problem can collect the pollen after sprouting after a kind of pollen liquid is sprouted
Device, its concrete technical scheme is：
A kind of pollen liquid sprouts device, including insulating box, wet box, mounting table, culture plate, and the wet box is placed on constant temperature
In case；Added with quantitative clear water in wet box；It is provided with wet box on mounting table, mounting table and is placed with culture plate.
The present invention sprouts for the liquid of pollen provides a kind of special equipment, simple in construction, it is ensured that it is suitable that liquid is sprouted
Humiture environment, after sprouting can conveniently and it is effective collect sprout after pollen.
Graduated scale is provided with the wet box；Clear water is added to warm box cassette bottom, and the water surface is high 1 centimetre, and mounting table is placed in cassette bottom；
Structure in wet box can also be divided into tank and non-irrigated groove, tank having clear water in wet box；Non-irrigated groove has mounting table.The structure makes wet
The internal environment of the suitable wet box of clear water amount added in box.
The present invention, which is provided, can increase the disengagement area that wet gauze increases water, make moisture evenly.Specially wet gauze is placed on
On mounting table, and wet gauze both sides are allowed to hang down to the clear water of cassette bottom；Can also be by non-irrigated groove mounting table set bottom in
Tank, wet gauze is provided with table top, and the other end is placed in tank.
The present invention also provides the structure of two kinds of mounting tables, and the mounting table can be that level meter, mounting table are embedded on table top
Four pin are provided with height regulation pin；Can also be mounting table and the affixed one of wet box, mounting table is parallel with wet box cassette bottom, wet box upper table
Face is provided with level meter.Mounting table is easy to make and installed when being independent structure, and mounting table and the affixed integral structure of wet box
Make overall more firm, easy loading or unloading operation.
Hygrothermograph is installed in wet box.
Brief description of the drawings
Fig. 1 is overall structure figure of the present invention.
Fig. 2 is the wet box cut-away view of example one；
Fig. 3 is the wet box cut-away view of example two；
Fig. 4 is the use state schematic diagram of example two.
It is insulating box 1, wet box 2, graduated scale 2-1, mounting table 3, height regulation pin 3-1, culture plate 4, hygrothermograph 5, wet in figure
Gauze 6, level meter 7, tank 8, non-irrigated groove 9.
Embodiment
The device that a suitable pollen liquid of Experience Design of the present invention in experiment is sprouted, it is ensured that constant temperature
Degree, humidity and oxygen, realize that the liquid of pollen is sprouted.
The present invention is illustrated below in conjunction with the accompanying drawings, a kind of pollen liquid as shown in Figures 1 to 4 sprouts device, including
Insulating box 1, wet box 2, mounting table 3, culture plate 4, the wet box 2 are placed in insulating box 1, wet added with quantitative clear water in wet box 2
It is provided with box 2 on mounting table 3, mounting table 3 and is placed with culture plate 4.
Insulating box 1 and wet box 2, which coordinate, in the present invention ensures that pollen germination possesses stable temperature and humidity.Especially in pollen
Sprouting initial stage, the change of temperature on sprout influence it is very big when, switch gate insulating box 1, internal temperature just has larger
Change, and at pollen germination initial stage, the change of temperature is very big on sprouting influence, the water in wet box 2 not only possesses holding humidity
Effect, can also slow down in the temperature change in wet box 2, wet box 2 has enough spaces to make pollen germination and pollen tube growth,
Pollen after can collect sprouting at the end of sprouting by way of filtering.
Clear water can be added directly into the cassette bottom of wet box 2, and wet box 2 can also be divided into tank 8 and the non-irrigated two parts space of groove 9,
Clear water is added in tank 8, mounting table 3 is set in non-irrigated groove 9.
Gauze water absorbing capacity is strong, increases gauze in this programme, wet gauze 6 is placed on mounting table 3, and by wet gauze 6
Edge is put into clear water, can increase the disengagement area of water, makes wet interior water humidity uniform.If the wet box that clear water is added directly into
2 cassette bottoms, then hang down the both sides of wet gauze 6 into wet box cassette bottom water；As clear water has been added in tank 8, then by putting in non-irrigated groove 9
The bottom of the setting of platform 3 is put in tank 8, its other end is placed in tank 8 after wet gauze 6 is placed on mounting table 3.
Mounting table 3 of the present invention can be the separate part that four pin are provided with height regulation pin 3-1；Can also be and wet box
2 cassette bottoms are parallel, and with the affixed integral part of wet box 2.
The present invention ensures that pollen germination liquid is evenly laid out on culture plate in Tissue Culture Plate below using level meter 7, water
Level 7 can be embedded on the table top of mounting table 3, with height regulation pin 3-1 leveling mounting table 3；The upper table of wet box 2 can also be arranged on
Face.
In order that being provided with graduated scale 2-1 in more convenient operation, the wet box 2, the water of addition is intuitively found out when making to add water
Face height.
Water water surface elevation added by the wet box cassette bottom is preferably 1 centimetre, and this is most suitable water.
Hygrothermograph 5 is provided with the wet box 2, makes the interior environment of sprouting of box more directly perceived, can be according to instruction adjustment water or temperature.
The use process of the present invention is described by taking example one as an example：
1) added water into wet box, with reference to graduated scale, add the high water of about 1cm；
2) reference level instrument, adjusts height regulation pin, mounting table is adjusted into level；
3) gauze is soaked, and then takes on mounting table, and the two ends of gauze are immersed in the water of wet box, on mounting table table top
Gauze keeps smooth；
4) pollen is mixed by a certain percentage with germination fluid,
5) the pollen germination liquid after mixing is uniformly coated onto on Tissue Culture Plate；
6) lid of wet box is covered, constant incubator is put into.
In above-mentioned steps (4), because pollen germination has density effect, therefore the mixing of pollen and germination fluid needs necessarily
Ratio, specific ratio can be sprouted using solid grope.
Germination fluid can not be too many in above-mentioned steps (5), did not had culture plate just, and liquid is too many, and water layer will be blocked up,
Pollen will sink to bottom, it is impossible to air contact, so as to reduce the germination rate of pollen
In above-mentioned steps (6), the different pollen temperature required when sprouting is different, for groping for temperature conditionss
It can be completed by way of solid is sprouted.
The lid of wet box 2 can not encrypt strip of paper used for sealing, it is ensured that air can circulate.This device is sprouted for pollen liquid,
It is without being adhered to after pollen germination expansion on culture medium, realize the pollen collected after sprouting, be that scientific data is provided with criterion
This.
For the collection of pollen after sprouting, the structure of different plant species pollen is different, and the time required for sprouting is also different,
The wall of such as paddy pollen is very thin, and its sprout time is just shorter, and the pollen wall of the conventional model plant arabidopsis in laboratory
It is slightly thicker, therefore the time sprouted is just longer；The time of sprouting has outside the Pass except the structure with pollen in itself, also with
The formula of nutrient solution is relevant, specifically to be defined the need for experimenter.
The present invention has advantages below：1st, stable humidity is ensured using wet box, incubator ensures stationary temperature, so that
Realize that the stable liquid of pollen is sprouted.2nd, the pollen after sprouting can be collected conveniently, be provided for the research of sexual plant reproduction
Research material.3rd, spare and accessory parts used in device are simple and reliable, reduce construction cycle and the manufacturing cost of product.
Claims (9)
1. a kind of pollen liquid sprouts device, it is characterized in that：Including insulating box, wet box, mounting table, culture plate, the wet box is put
Put in insulating box；Added with quantitative clear water in wet box；It is provided with wet box on mounting table, mounting table and is placed with culture plate.
2. pollen liquid as claimed in claim 1 sprouts device, it is characterized in that：Graduated scale is provided with the wet box；Clear water is added
To warm box cassette bottom, the water surface is high 1 centimetre, and mounting table is placed in cassette bottom.
3. pollen liquid as claimed in claim 1 sprouts device, it is characterized in that：Provided with tank and non-irrigated groove, tank in the wet box
Inside there is clear water；Mounting table is provided with non-irrigated groove.
4. pollen liquid as claimed in claim 2 sprouts device, it is characterized in that：Mounting table is provided with wet gauze, the wet gauze
Hang down into wet box cassette bottom water both sides.
5. pollen liquid as claimed in claim 3 sprouts device, it is characterized in that：Mounting table, which is highly less than on tank, mounting table, to be set
There is wet gauze, the wet gauze other end is placed in tank.
6. pollen liquid sprouts device as described in claim 4 or 5, it is characterized in that：Level meter is embedded on mounting table table top, is placed
The pin of platform four is provided with height regulation pin.
7. pollen liquid sprouts device as described in claim 4 or 5, it is characterized in that：Mounting table and the affixed one of wet box, mounting table
Parallel with wet box cassette bottom, wet box upper surface is provided with level meter.
8. pollen liquid as claimed in claim 6 sprouts device, it is characterized in that：Hygrothermograph is installed in wet box.
9. pollen liquid as claimed in claim 7 sprouts device, it is characterized in that：Hygrothermograph is installed in wet box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710783925.1A CN107325969B (en) | 2017-09-04 | 2017-09-04 | Pollen liquid germination device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710783925.1A CN107325969B (en) | 2017-09-04 | 2017-09-04 | Pollen liquid germination device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107325969A true CN107325969A (en) | 2017-11-07 |
| CN107325969B CN107325969B (en) | 2020-12-01 |
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ID=60204777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710783925.1A Active CN107325969B (en) | 2017-09-04 | 2017-09-04 | Pollen liquid germination device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107325969B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110591914A (en) * | 2019-09-27 | 2019-12-20 | 佛山科学技术学院 | Pollen culture room |
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2017
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| CN103571789A (en) * | 2013-11-13 | 2014-02-12 | 江苏省农业科学院 | Jasmine pollen in-vitro germination liquid culture medium and method for measuring activity of jasmine pollen |
| CN105191581A (en) * | 2015-10-22 | 2015-12-30 | 山西省农业科学院园艺研究所 | Method for obtaining pear pollen for pollination |
| CN106520549A (en) * | 2016-12-28 | 2017-03-22 | 广东工业大学 | Source component and device for inducing high-voltage impulse wave to separate single cells through laser photolysis |
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Cited By (1)
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| CN110591914A (en) * | 2019-09-27 | 2019-12-20 | 佛山科学技术学院 | Pollen culture room |
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| Publication number | Publication date |
|---|---|
| CN107325969B (en) | 2020-12-01 |
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Application publication date: 20171107 Assignee: Nucleosome (Beijing) Biotechnology Co.,Ltd. Assignor: NORTH CHINA University OF SCIENCE AND TECHNOLOGY Contract record no.: X2023110000079 Denomination of invention: Liquid pollen germination device Granted publication date: 20201201 License type: Common License Record date: 20230616 |