CN207175973U - Pollen liquid sprouts device - Google Patents

Abstract

The utility model provides a kind of pollen liquid and sprouts device, including insulating box, wet box, mounting table, culture plate, the wet box are placed in insulating box；Added with quantitative clear water in wet box；Mounting table is provided with wet box, culture plate is placed with mounting table, clear water can be added directly into wet box cassette bottom, wet box can also be divided into tank and non-irrigated groove two parts space, clear water is added in tank, mounting table is set in non-irrigated groove, if the wet box cassette bottom that clear water is added directly into, the both sides of wet gauze are hung down into wet box cassette bottom water；As clear water has been added in tank, the bottom that then mounting table in non-irrigated groove is set is in tank, its other end is placed in tank after wet gauze is placed on mounting table, mounting table can be separate part, can also be with the affixed one of wet box, the utility model for pollen liquid sprout provides a kind of special equipment, it is simple in construction, ensure the suitable humiture environment that liquid is sprouted, pollen after sprouting can conveniently and be effectively collected after sprouting.

Description

Pollen liquid sprouts device

Technical field

It the utility model is related to a kind of pollen liquid and sprout device, particularly a kind of pollen liquid for scientific experiment is sprouted Transmitting apparatus.

Background technology

The sexual reproduction of plant is always the hot issue of scientific research field, and the extension of pollen germination and pollen tube has been Into the committed step of plant fertilization, therefore test, collected necessary to pollen after sprouting is scientific research in vitro.But It is to be completed at present on the sprouting of pollen more on solid medium, the pollen after sprouting is collected less than seriously substantially The research to plant fertilization process is hindered, therefore, there is an urgent need to a kind of mode of Liquid Culture, the pollen after sprouting can lead to The mode of filtering is collected.It is many to influence the factor of pollen germination, in addition to ionic environment, also temperature, humidity and oxygen, Gu These conditions of body culture medium are relatively easy to control, and fluid nutrient medium, and due to long the time required to pollen germination, therefore condition is very Hardly possible control, especially humidity, the space of incubator is larger, and the change of its humidity is also relatively rapider.In current utility model, do not have Device in terms of pollen liquid sprouting.

Utility model content

The technical problems to be solved in the utility model is that pollen liquid sprouts collection less than the flower after sprouting in the prior art Powder.

The utility model provides the flower after sprouting can be collected after a kind of pollen liquid is sprouted to solve above-mentioned technical problem The device of powder, its concrete technical scheme are：

A kind of pollen liquid sprouts device, including insulating box, wet box, mounting table, culture plate, the wet box are placed on constant temperature In case；Added with quantitative clear water in wet box；Mounting table is provided with wet box, culture plate is placed with mounting table.

The utility model is sprouted for the liquid of pollen provides a kind of special equipment, simple in construction, ensures what liquid was sprouted Suitable humiture environment, pollen after sprouting can conveniently and be effectively collected after sprouting.

Graduated scale is provided with the wet box；Clear water is added to warm box cassette bottom, and the water surface is high 1 centimetre, and mounting table is placed in cassette bottom； Structure in wet box can also be divided into tank and non-irrigated groove in wet box, have clear water in tank；Non-irrigated groove has mounting table.The structure makes wet The internal environment of the suitable wet box of clear water amount added in box.

The utility model, which provides, can increase the disengagement area that wet gauze increases water, make moisture evenly.Specially wet gauze It is placed on mounting table, and allows wet gauze both sides to hang down to the clear water of cassette bottom；Can also be that the mounting table in non-irrigated groove is set Bottom is provided with table top in tank, wet gauze, and the other end is placed in tank.

The utility model also provides the structure of two kinds of mounting tables, and the mounting table can be that level meter is embedded on table top, put Put the pin of platform four and be provided with height regulation pin；Can also be mounting table and the affixed one of wet box, mounting table is parallel with wet box cassette bottom, wet box Upper surface is provided with level meter.Mounting table is easy to make and installed when being independent structure, and mounting table and wet box are affixed integral Structure makes overall more firm, easy loading or unloading operation.

Hygrothermograph is installed in wet box.

Brief description of the drawings

Fig. 1 is the utility model of the whole structure diagram；

Fig. 2 is the wet box cut-away view of example one；

Fig. 3 is the wet box cut-away view of example two；

Fig. 4 is the use state diagram of example two.

It is insulating box 1, wet box 2, graduated scale 2-1, mounting table 3, height regulation pin 3-1, culture plate 4, hygrothermograph 5, wet in figure Gauze 6, level meter 7, tank 8, non-irrigated groove 9.

Embodiment

The device that a suitable pollen liquid of Experience Design of the utility model in experiment is sprouted, ensures constant Temperature, humidity and oxygen, realize that the liquid of pollen is sprouted.

The utility model is illustrated below in conjunction with the accompanying drawings, a kind of pollen liquid as shown in Figures 1 to 4 sprouts device, Including insulating box 1, wet box 2, mounting table 3, culture plate 4, the wet box 2 is placed in insulating box 1, added with quantitatively clear in wet box 2 Water, wet box 2 is interior to be provided with mounting table 3, and culture plate 4 is placed with mounting table 3.

Insulating box 1 and wet box 2, which coordinate, in the utility model ensures that pollen germination possesses stable temperature and humidity.Especially exist Pollen germination initial stage, when the change of temperature influences very big to sprouting, switch gate insulating box 1, internal temperature just have compared with Big change, and at pollen germination initial stage, the change of temperature influence very big on sprouting, and the water in wet box 2 not only possesses holding The effect of humidity, the temperature change in wet box 2 can also be slowed down, there are enough spaces to make pollen germination and pollen tube in wet box 2 Growth, pollen after can collect sprouting at the end of sprouting by way of filtering.

Clear water can be added directly into the cassette bottom of wet box 2, and wet box 2 can also be divided into tank 8 and two parts space of non-irrigated groove 9, Clear water is added in tank 8, mounting table 3 is set in non-irrigated groove 9.

Gauze water absorbing capacity is strong, increases gauze in this programme, and wet gauze 6 is placed on mounting table 3, and by wet gauze 6 Edge is put into clear water, can increase the disengagement area of water, makes wet interior water humidity uniform.If the wet box that clear water is added directly into 2 cassette bottoms, then the both sides of wet gauze 6 are hung down into wet box cassette bottom water；As clear water has been added in tank 8, then by putting in non-irrigated groove 9 The bottom of the setting of platform 3 is put in tank 8, its other end is placed in tank 8 after wet gauze 6 is placed on mounting table 3.

Mounting table 3 described in the utility model can be the separate part that four pin are provided with height regulation pin 3-1；Can also be with The cassette bottom of wet box 2 is parallel, and with the affixed integral part of wet box 2.

The utility model ensures that pollen germination liquid is evenly laid out in culture plate in Tissue Culture Plate below using level meter 7 On, level meter 7 can be embedded on the table top of mounting table 3, with height regulation pin 3-1 leveling mounting table 3；Wet box 2 can also be arranged on Upper surface.

In order that more convenient operation, the wet box 2 is interior to be provided with graduated scale 2-1, makes the water that addition is intuitively found out when adding water Face height.

Water water surface elevation added by the wet box cassette bottom is preferably 1 centimetre, and this is most suitable water.

Hygrothermograph 5 is provided with the wet box 2, makes the interior environment of sprouting of box more directly perceived, can be according to instruction adjustment water or temperature.

Describe of the present utility model to use process by taking example one as an example：

1) add water into wet box, with reference to graduated scale, add the high water of about 1cm；

2) reference level instrument, height regulation pin is adjusted, mounting table is adjusted to level；

3) gauze is soaked, and then takes on mounting table, and the both ends of gauze are immersed in the water of wet box, on mounting table table top Gauze keeps smooth；

4) pollen is mixed by a certain percentage with germination fluid,

5) the pollen germination liquid after mixing is uniformly coated onto on Tissue Culture Plate；

6) lid of wet box is covered, is put into constant incubator.

In above-mentioned steps (4), because pollen germination has density effect, therefore the mixing of pollen and germination fluid needs necessarily Ratio, specific ratio can be sprouted using solid grope.

Germination fluid can not be too many in above-mentioned steps (5), did not had culture plate just, and liquid is too many, and water layer will be blocked up, Pollen will sink to bottom, it is impossible to air contact, so as to reduce the germination rate of pollen

In above-mentioned steps (6), the different pollen temperature required when sprouting is different, for groping for temperature conditionss It can be completed by way of solid sprouting.

The lid of wet box 2 can not encrypt strip of paper used for sealing, it is ensured that air can circulate.This device is sprouted for pollen liquid, It is without being adhered to after pollen germination expansion on culture medium, realize the pollen collected after sprouting, criterion is provided with for scientific data This.

Collection for pollen after sprouting, the structure of different plant species pollen is different, and the time required for sprouting is also different, For example the wall of paddy pollen is very thin, its sprout time is with regard to shorter, and the pollen wall for the model plant arabidopsis that laboratory is commonly used It is slightly thicker, therefore the time sprouted is with regard to longer；The time of sprouting has outside the Pass except the structure with pollen in itself, also with The formula of nutrient solution is relevant, and specifically the needs by experimenter are defined.

The utility model has advantages below：1st, stable humidity is ensured using wet box, incubator ensures stationary temperature, So as to realize that the stable liquid of pollen is sprouted.2nd, the pollen after sprouting can be collected conveniently, be carried for the research of sexual plant reproduction Research material is supplied.3rd, spare and accessory parts used in device are simple and reliable, reduce construction cycle and the manufacturing cost of product.

Claims (9)

1. a kind of pollen liquid sprouts device, it is characterized in that：Including insulating box, wet box, mounting table, culture plate, the wet box is put Put in insulating box；Added with quantitative clear water in wet box；Mounting table is provided with wet box, culture plate is placed with mounting table.

2. pollen liquid as claimed in claim 1 sprouts device, it is characterized in that：Graduated scale is provided with the wet box；Clear water adds To warm box cassette bottom, the water surface is high 1 centimetre, and mounting table is placed in cassette bottom.

3. pollen liquid as claimed in claim 1 sprouts device, it is characterized in that：Tank and non-irrigated groove, tank are provided with the wet box Inside there is clear water；Mounting table is provided with non-irrigated groove.

4. pollen liquid as claimed in claim 2 sprouts device, it is characterized in that：Mounting table is provided with wet gauze, the wet gauze Hang down into wet box cassette bottom water both sides.

5. pollen liquid as claimed in claim 3 sprouts device, it is characterized in that：Mounting table is highly less than tank, is set on mounting table There is wet gauze, the wet gauze other end is placed in tank.

6. pollen liquid sprouts device as described in claim 4 or 5, it is characterized in that：Level meter is embedded on mounting table table top, is placed The pin of platform four is provided with height regulation pin.

7. pollen liquid sprouts device as described in claim 4 or 5, it is characterized in that：Mounting table and the affixed one of wet box, mounting table Parallel with wet box cassette bottom, wet box upper surface is provided with level meter.

8. pollen liquid as claimed in claim 6 sprouts device, it is characterized in that：Hygrothermograph is installed in wet box.

9. pollen liquid as claimed in claim 7 sprouts device, it is characterized in that：Hygrothermograph is installed in wet box.