CN104988055B - A kind of device of the culture of microalgae adherence formula and liquid submersion culture conversion - Google Patents
A kind of device of the culture of microalgae adherence formula and liquid submersion culture conversion Download PDFInfo
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- CN104988055B CN104988055B CN201510478010.0A CN201510478010A CN104988055B CN 104988055 B CN104988055 B CN 104988055B CN 201510478010 A CN201510478010 A CN 201510478010A CN 104988055 B CN104988055 B CN 104988055B
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- microalgae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/08—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by vibration
Abstract
A kind of device of the culture of microalgae adherence formula and liquid submersion culture conversion, it is related to a kind of device for cultivating microalgae.It will solve realize the problem of repeatedly being changed repeatedly between liquid submersion culture and wall-attaching type culture at present.Device includes thickener, communicating pipe, drip irrigation pipe, concave-board, rotatable support frame, collecting box.Advantages of the present invention：Cost is low, simple to operate, it is easy to safeguard；Solve immersion culture presence because algae is mutually covered, caused optical attenuation phenomenon, while solving CO2Solubility in water body is relatively low, limits CO2The deficiency of absorption efficiency；Realize the culture of microalgae adherence formula and liquid submersion culture conversion, while microalgae high carbon sequestration efficiency is realized, realize fast high-density culture, effectively improve the growth rate of microalgae, reduce the toxigenic capacity of microalgae, during microdisk electrode to highest biomass, it is placed in thickener and reproduce said process, realize repetitious switch target.
Description
Technical field
The present invention relates to a kind of device for cultivating microalgae.
Background technology
Microalgae is to carry out putting oxygen, photosynthetic lower hydrobiont, and its is simple in construction, and breeding is rapid, to the sun
Energy utilization rate is high, and the ability with GHG emissions mitigation (carbon dioxide) is strong to environmental suitability, is considered as third generation biomass
Energy source raw material.
Cultivate and use liquid submersion culture microalgae more, water consumption is huge, and there is light and propagated in water body and decay
And CO2The problems such as solubility in water body is relatively low, limits chlorella carbon sequestration efficiency and efficiency of light absorption.Adhere-wall culture is utilized
The adherent characteristic of frustule, reduces water consumption so that CO2Can fully it be contacted with frustule, CO2Utilization rate significantly improve,
And the conduction of light is transmitted without longer water body, and the efficiency of light energy utilization is significantly improved, but micro algae growth rate is less than liquid submersion
Culture.
Current wall-attaching type and liquid submersion training method are substantially independent progress, do not occur realizing that liquid submersion is trained
Support the device repeatedly changed repeatedly between wall-attaching type culture.
The content of the invention
It is repeatedly anti-between liquid submersion culture and wall-attaching type culture the invention aims to solve not realizing at present
The problem of changing again, and a kind of device of microalgae adherence formula culture and liquid submersion the culture conversion provided.
A kind of device of the culture of microalgae adherence formula and liquid submersion culture conversion, it includes thickener, communicating pipe, drip irrigation
Pipe, concave-board, rotatable support frame, collecting box；The placement direction of the concave-board is horizontal by 5-15 ° of angle；It is described recessed
Frid is made up of the flexible liquid permeable plate of material in upper surface and the impermeable plate of material of the rigid liquid of lower surface；On described
The liquid permeable material plate surface of surface flexible has a construction unit of projection, and the row that extends transversely and prolongs along longitudinal direction
The row stretched constitute building block array；The cross section of the construction unit is circular, square, rectangle, ellipse, triangle or water chestnut
Shape；The construction unit mutually equidistantly arranges or increased successively spacing in the same row；The building block array it is adjacent
Line space between row is linearly changed with constant increment increase or increment；The row extended transversely is in the horizontal direction
On into same angle extension, the row extended longitudinally in vertical direction into same angle extend.
Operation principle of the present invention：The device for the culture of microalgae adherence formula and liquid submersion the culture conversion that the present invention is provided,
Operation principle：Liquid containing microalgae is concentrated by thickener using hyperfiltration technique, then flows into communicating pipe, enters back into drip irrigation
Pipe, the microalgae flowed out through drip irrigation pipe is attached to concave-board upper surface, realizes liquid submersion cultivation conditions to wall-attaching type culture shape
The transformation of state, by the concussion of rocking of rotatable support frame, realizes that the microalgae for being attached to concave-board upper surface is shaken back by rocking
Liquid submersion cultivation conditions are grouped into, collecting box realizes the collection of algae solution to store the microalgae come off from concave-board upper surface；
When microdisk electrode to highest biomass, it is placed in thickener and reproduce said process, realize repetitious switch target.
Advantages of the present invention：
What the 1st, the present invention was provided realizes the device of the culture of microalgae adherence formula and liquid submersion culture conversion, and cost is low, behaviour
Make simple, it is easy to safeguard.
2nd, using the present invention device culture microalgae, solve immersion culture presence because algae is mutually covered, cause
Optical attenuation phenomenon, while solve CO2Solubility in water body is relatively low, limits CO2The deficiency of absorption efficiency.
3rd, using the device culture microalgae of the present invention, the micro algae growth rate for solving wall-attaching type culture presence slower is asked
Topic.
4th, using the device culture microalgae of the present invention, in the micro algae growth stage, the culture of microalgae adherence formula and liquid are realized
Immersion culture is changed, and has given full play to the advantage of liquid submersion culture and wall-attaching type culture, is realizing the high carbon sequestration effect of microalgae
While rate, fast high-density culture is realized, the growth rate of microalgae is effectively improved, the toxigenic capacity of microalgae is reduced.
5th, using the device culture microalgae of the present invention, during microdisk electrode to highest biomass, it is placed in thickener and reproduce
Said process, realizes repetitious switch target.
Brief description of the drawings
Fig. 1 is the structural representation of the culture of microalgae adherence formula and liquid submersion culture conversion equipment in the present invention, wherein 1
It is communicating pipe for thickener, 2,3 be drip irrigation pipe, 4 be concave-board, 5 be rotatable support frame, 6 is collecting box；
Fig. 2 is the diagrammatic side-view cross-sectional of the culture of microalgae adherence formula and liquid submersion culture conversion equipment concave frid in the present invention
Figure, wherein 7 being the flexible liquid permeable plate of material in upper surface, 8 being the impermeable plate of material of the rigid liquid of lower surface；
Fig. 3 is the culture of microalgae adherence formula and liquid submersion culture conversion equipment concave frid upper surface in embodiment 1
Schematic diagram, wherein being 9 construction units；
Fig. 4 is the culture of microalgae adherence formula and liquid submersion culture conversion equipment concave frid upper surface in embodiment 2
Schematic diagram, wherein being 9 construction units.
Embodiment
Technical solution of the present invention is not limited to act embodiment set forth below, in addition between each embodiment
Any combination.
Embodiment one：With reference to shown in Fig. 1, Fig. 2, a kind of microalgae adherence formula culture of present embodiment and liquid-immersed
The device of formula culture conversion, it includes thickener 1, communicating pipe 2, drip irrigation pipe 3, concave-board 4, rotatable support frame 5, collecting box 6；Institute
The placement direction of concave-board 4 is stated horizontal by 5-15 ° of angle；The concave-board 4 liquid flexible by upper surface can pass through
Property plate of material 7 and the impermeable plate of material 8 of the rigid liquid of lower surface are constituted；The flexible liquid permeable material in the upper surface
There is the construction unit 9 of projection on the surface of plate 7, and the row gone and extended longitudinally extended transversely constitute building block array；
The cross section of the construction unit 9 is circular, square, rectangle, ellipse, triangle or rhombus；The construction unit 9 is same
Mutually equidistantly arranged in row or increase spacing successively；Line space between the adjacent lines of the building block array is with constant
Increment increases or increment linearly changes；The row extended transversely extends into same angle in the horizontal direction, along vertical
Extend in vertical direction into same angle to the row of extension.
Embodiment two：Present embodiment from unlike embodiment one, the exit of thickener 1 stream
Go out for the algae solution after concentration, the operating pressure of thickener 1 is 0.10-0.20MPa.Other steps and parameter and specific embodiment party
Formula one is identical.
Embodiment three：Present embodiment from unlike embodiment one or two, the drip irrigation pipe 3, bear
Algae solution is laid in the upper surface of concave-board 4 by duty, realizes liquid submersion cultivation conditions to the transformation of wall-attaching type cultivation conditions, drop
The nominal diameter for filling pipe 3 is 12-16mm.Other steps and parameter are identical with embodiment one or two.
Embodiment four：Unlike one of present embodiment and embodiment one to three, the concave-board 4
Be placed with drip irrigation pipe 3 one section is placed in highest point, and the speed of the outflow algae solution of drip irrigation pipe 3 is 0.008ml/s.Other steps and parameter
It is identical with one of embodiment one to three.
Embodiment five：It is described rotatable unlike one of present embodiment and embodiment one to four
The two ends of support 5 are connected to concave-board 4 and collecting box 6, to realize the rotation of anchor frid 4, and will attach to recessed
The microalgae of the upper surface of frid 4 revert to liquid submersion cultivation conditions by rocking concussion.Other steps and parameter and specific reality
Apply one of mode one to four identical.
Embodiment six：It is described rotatable unlike one of present embodiment and embodiment one to five
The rotating speed of support 5 is 100-300r/min, and material is 301 stainless steels, 304 stainless steels, 312 stainless steels or 316 stainless steels.It is other
Step and parameter are identical with one of embodiment one to five.
Embodiment seven：Unlike one of present embodiment and embodiment one to six, the collecting box 6
To store the microalgae come off from the upper surface of concave-board 4, the collection of algae solution is realized, length-width ratio is (1-8):1, a height of 0.2-
0.35m.Other steps and parameter are identical with one of embodiment one to six.
Embodiment eight：Unlike one of present embodiment and embodiment one to seven, the structure list
The height of member 9 is 5-20mm.Other steps and parameter are identical with one of embodiment one to seven.
Embodiment nine：It is described along longitudinal direction unlike one of present embodiment and embodiment one to eight
Construction unit 9 in the row of extension is of the same size.One of other steps and parameter and embodiment one to eight phase
Together.
Embodiment ten：It is described along longitudinal direction unlike one of present embodiment and embodiment one to nine
The size of construction unit 9 in the row of extension can also be reduced with constant reduction amount or reduction amount linearly changes.Other steps
Rapid and parameter is identical with one of embodiment one to nine.
Embodiment 11：Unlike one of present embodiment and embodiment one to nine, the upper table
The material of the flexible liquid permeable plate of material 7 in face is glass fibre, nylon, cotton, fiber crops, carbon fiber, synthetic fibers, sponge,
One or more materials in plastic foam are made.
It is mixed by any ratio when material is made for multiple material in present embodiment.
Beneficial effects of the present invention are verified using following examples：
Embodiment 1：
With reference to shown in Fig. 1, Fig. 2 and Fig. 3, the culture of microalgae adherence formula and liquid submersion culture provided using the present invention is turned
The device changed, the wherein operating pressure of thickener 1 are 0.15MPa；The nominal diameter of drip irrigation pipe 3 is 16mm；Drip irrigation pipe 3 flows out algae
The speed of liquid is 0.008ml/s；The material of the flexible liquid permeable plate of material 7 in upper surface is glass fibre, and size is
300mm×300mm；The cross section of construction unit 9 is circle, is highly 5mm.
Algae solution volume is 500L in incubator, and initial concentration is 1 × 109Individual/ml is dense after concentrated machine 1 is concentrated 3 hours
Degree is changed into 7.342 × 1010Individual/ml, concentrate is 6.8L；The upper surface of concave-board 4 is paved with after 1 hour；By concentrate with titrating
The low concentration algae solution slided in journey from concave-board 4 collects mixing, and cumulative volume is about 500L, and it is 6.3 × 10 to measure concentration of algae7Individual/
ml。
The ratio that the algae amount being attached on concave-board 4 accounts for original total algae amount is：
After culture 1 day, the algae attached on concave-board 4 is put into the low concentration algae solution of collection and cleaned, rotating speed is 150r/
Min, the concentration of measurement algae is 4.5 × 109Individual/ml, algae solution cumulative volume is 499L, and Volume Changes are little.
The increased times of algae are：
It can be seen that, it can improve the growth rate of microalgae, ideal adherent effect using apparatus of the present invention.
Embodiment 2：
With reference to shown in Fig. 1, Fig. 2 and Fig. 4, the culture of microalgae adherence formula and liquid submersion culture provided using the present invention is turned
The device changed, the wherein operating pressure of thickener 1 are 0.15MPa；The nominal diameter of drip irrigation pipe 3 is 16mm；Drip irrigation pipe 3 flows out algae
The speed of liquid is 0.008ml/s；The material of the flexible liquid permeable plate of material 7 in upper surface is glass fibre, and size is
300mm×300mm；The cross section of construction unit 10 is rhombus, is highly 6mm.
The ratio that algae amount on measurement concave-board 4 accounts for original total algae amount is：
After culture 1 day, the algae attached on concave-board 4 is put into the low concentration algae solution of collection and cleaned, measure the concentration of algae
For 3.0/ml, algae solution cumulative volume is 499L, and Volume Changes are little.
The increased times of algae are：
It can be seen that, it can improve the growth rate of microalgae, ideal adherent effect using apparatus of the present invention.
Claims (6)
1. a kind of microalgae adherence formula culture and liquid submersion culture conversion device, it is characterised in that it include thickener (1),
Communicating pipe (2), drip irrigation pipe (3), concave-board (4), rotatable support frame (5), collecting box (6)；The placement direction of the concave-board (4)
Horizontal by 5-15 ° of angle；The concave-board (4) is by upper surface flexible liquid permeable plate of material (7) and following table
The impermeable plate of material of the rigid liquid in face (8) is constituted；Flexible liquid permeable plate of material (7) surface in the upper surface has convex
The construction unit (9) risen, and the row gone and extended longitudinally extended transversely constitute building block array；The structure list
The cross section of first (9) is circular, square, rectangle, ellipse, triangle or rhombus；The construction unit (9) phase in the same row
Equidistantly arrange or increase spacing successively；Line space between the adjacent lines of the building block array is increased with constant increment
Big or increment linearly changes；The row extended transversely extends into same angle in the horizontal direction, extends longitudinally
Row in vertical direction into same angle extend；
Thickener (1) exit outflow for the algae solution after concentration, the operating pressure of thickener (1) is 0.10-
0.20MPa；
The drip irrigation pipe (3), is responsible for algae solution being laid in the upper surface of concave-board (4), realizes that liquid submersion cultivation conditions are arrived
The transformation of wall-attaching type cultivation conditions, the nominal diameter of drip irrigation pipe (3) is 12-16mm；
The concave-board (4) is placed with one section of drip irrigation pipe (3) and is placed in highest point, and the speed of drip irrigation pipe (3) outflow algae solution is
0.008ml/s；
The two ends of the rotatable support frame (5) are connected to concave-board (4) and collecting box (6), to realize anchor frid
(4) rotation, and will attach to the microalgae of concave-board (4) upper surface and revert to liquid submersion culture shape by rocking concussion
State.
2. a kind of microalgae adherence formula culture according to claim 1 and the device of liquid submersion culture conversion, its feature
The rotating speed for being the rotatable support frame (5) is 100-300r/min, and material is 301 stainless steels, 304 stainless steels, 312 stainless steels
Or 316 stainless steels.
3. a kind of microalgae adherence formula culture according to claim 1 and the device of liquid submersion culture conversion, its feature
It is that the collecting box (6), to store the microalgae come off from concave-board (4) upper surface, realizes the collection of algae solution, length-width ratio is
(1-8):1, a height of 0.2-0.35m.
4. a kind of microalgae adherence formula culture according to claim 1 and the device of liquid submersion culture conversion, its feature
The height for being the construction unit (9) is 5-20mm.
5. a kind of microalgae adherence formula culture according to claim 1 and the device of liquid submersion culture conversion, its feature
It is that the construction unit (9) in the row extended longitudinally is of the same size.
6. a kind of microalgae adherence formula culture according to claim 1 and the device of liquid submersion culture conversion, its feature
Being the size of the construction unit (9) in the row extended longitudinally can also be reduced or reduction amount line with constant reduction amount
Change to property.
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| CN201510478010.0A CN104988055B (en) | 2015-08-06 | 2015-08-06 | A kind of device of the culture of microalgae adherence formula and liquid submersion culture conversion |
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| CN201510478010.0A CN104988055B (en) | 2015-08-06 | 2015-08-06 | A kind of device of the culture of microalgae adherence formula and liquid submersion culture conversion |
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| CN110903947A (en) * | 2019-12-20 | 2020-03-24 | 深圳泰利能源有限公司 | Open type photobioreactor for microalgae cultivation |
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| US4908128A (en) * | 1987-09-15 | 1990-03-13 | Envirocycle Pty. Ltd. | Composite bacteria support medium |
| US6197573B1 (en) * | 1998-11-17 | 2001-03-06 | Biocon India Limited | Solid state fermentation |
| CN101870950B (en) * | 2009-04-24 | 2013-09-04 | 中国石油化工股份有限公司 | Device for culturing microalgae |
| CN103289888A (en) * | 2012-03-01 | 2013-09-11 | 中国科学院青岛生物能源与过程研究所 | Inserting-plate type microalgae semi-dry solid adherent culture device |
| CN103396936B (en) * | 2013-07-30 | 2015-08-12 | 新奥科技发展有限公司 | A kind of photo bio cultivation unit and cultivating system |
| CN104328048B (en) * | 2014-10-30 | 2017-03-29 | 国家开发投资公司 | Superficial growth formula photosynthetic microorganism culture plate and its system |
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