CN112608845A

CN112608845A - Bionic stimulation device for mechanical pressure of stem cells

Info

Publication number: CN112608845A
Application number: CN202110030334.3A
Authority: CN
Inventors: 李广杰; 王记增
Original assignee: Lanzhou University; First Hospital of Lanzhou University
Current assignee: Lanzhou University; First Hospital of Lanzhou University
Priority date: 2021-01-11
Filing date: 2021-01-11
Publication date: 2021-04-06
Anticipated expiration: 2041-01-11
Also published as: CN112608845B

Abstract

The invention relates to the field of stem cell pressure stimulation methods, in particular to a mechanical pressure stress bionic stimulation device for stem cells. The problem of current mechanical stimulation direction unicity and stimulation inefficiency is solved. The method comprises the following steps: whole device uses the box as the installation main part, box internally mounted has the one-level centrifuge, and the one-level centrifuge drives a plurality of second grade centrifuges synchronous revolution of installation above that, and the extrusion group rotation of installation above that is driven to the second grade centrifuge, and the extrusion is organized the interior liquid bag and is passed through centrifugal force extrusion cell dish. The device adopts two centrifugal devices, makes the liquid bag carry out multi-direction extrusion to the cell dish through centrifugal force, realizes the omnidirectionality of mechanical stimulation, improves the accuracy of research, can once only realize simultaneously that cell stimulation in batches.

Description

Bionic stimulation device for mechanical pressure of stem cells

Technical Field

The invention relates to the field of stem cell pressure stimulation methods, in particular to a mechanical pressure stress bionic stimulation device for stem cells.

Background

Cells in a living body are in an environment full of various mechanical stimuli, the cells are stretched, contracted, twisted and the like by skeletal motion during motion, the digestive tract wriggles during eating, the shearing force caused by body fluid flow and the like, and even a simple finger-operated motion can bring the mechanical stimuli to the cells in the living body. As is known to all, exercise is beneficial to health, in vivo, normal biomechanical stimulation is also necessary for normal physiological processes such as bone tissue balance, embryonic development and the like, and research shows that different mechanical stimulation can have great influence on life activities such as growth, proliferation, differentiation and the like of cells, and abnormal biomechanical stimulation can also generate diseases such as osteoporosis and the like.

Tension and compression are the most common mechanical stimuli in vivo, and numerous researchers have been added to in vitro simulation studies of mechanical stimuli in cells in vivo for many years. The current research on the action of cells under tensile and compressive mechanical stimulation mainly includes two aspects: one is mechanical stimulation under two-dimensional culture conditions, which comprises two-point mechanical stretching, four-point mechanical stretching, pneumatic mechanical action and the like on an elastic membrane, and the mechanical stimulation is generated on cells cultured on the elastic membrane through the elastic action of the membrane, and the greatest defect of the loading mode is that the deformation of each part on the elastic membrane is not uniform; one is mechanical stimulation under three-dimensional culture conditions, including mechanical stimulation under fixed deformation, namely, research on the influence of fixed stress or strain conditions on cell culture, and the research belongs to cell culture research under static mechanical stimulation. The cell culture research under dynamic mechanical stimulation is mechanical stimulation realized by using a piezoelectric ceramic piece, a lead screw, a connecting rod mechanism and the like, but each device cannot realize mechanical stimulation in all directions, so that the inaccuracy of research data is easily caused. Meanwhile, the existing mechanical stimulation device has small one-time stimulation cell amount and low stimulation efficiency.

Disclosure of Invention

The invention provides a mechanical compressive stress bionic stimulation device for stem cells, which effectively solves the problems of single direction and low stimulation efficiency of the existing mechanical stimulation.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

a bionic stimulation device for mechanical pressure stress of stem cells takes a box body 1 as an installation main body, and is characterized in that a primary centrifuge 2 is installed inside the box body 1, the primary centrifuge 2 drives a plurality of secondary centrifuges 3 installed on the primary centrifuge to rotate, the secondary centrifuges 3 drive an extrusion group 4 installed on the secondary centrifuges to rotate, and a liquid bag 22 in the extrusion group 4 extrudes a cell plate 23 through centrifugal force.

The primary centrifuge 2 drives the fluted disc 8 to rotate through the primary motor 5 and the rotating rod 6.

Fluted disc 8 is movably installed on box 1 through one-level main shaft 7, is provided with spliced pole 9 and carousel 10 simultaneously on fluted disc 8.

The second-stage centrifuge 3 is mounted on the turntable 10.

The secondary centrifuge 3 takes a secondary box body 11 as an installation main body, a matching rotating device of a worm wheel 13 and a worm 14 is installed in the secondary box body 11, and the worm 14 is driven by a secondary motor 15; the worm wheel 13 is arranged on the secondary main shaft 12, one end of the secondary main shaft 12 is movably arranged on the secondary box body 11, the other end is provided with a main gear 16, the main gear 16 is mutually meshed with a driven gear 19 arranged on a driven shaft 18,

the driven shaft 18 is provided with the extrusion group 4.

The secondary main shaft 12 is stabilized by the aid of a mounting frame 17.

A plurality of squeezing boxes 20 are arranged in the squeezing group 4, and a liquid bag 22 and a cell tray 23 are arranged in each squeezing box 20.

The squeeze box 20 is provided with a bulkhead 21 between a fluid bag 22 and a cell tray 23.

The primary motor 5 and the secondary motor 1 are controlled by a controller 24.

The invention has the beneficial effects that: 1) this device is through the cooperation that adopts one-level centrifuge and second grade centrifuge, on the rotatory basis of one-level centrifuge, drives the rotation of second grade centrifuge for extrusion group on the second grade centrifuge is linear extrusion cell dish.

2) This device is through the cooperation of two centrifuges for the extrusion cell dish that the liquid bag can be multidirectional realizes multidirectional mechanics stimulation.

3) The extrusion group of the device is provided with a plurality of extrusion groups, and each extrusion group is internally provided with a plurality of extrusion discs, so that mechanical stimulation can be performed on a large number of stem cells at one time.

Drawings

FIG. 1 is a schematic view of the outer structure of the present invention;

FIG. 2 is a schematic view of the internal structure of the present invention;

FIG. 3 is a schematic view of a one-stage centrifuge mounting arrangement;

FIG. 4 is a schematic view of a two-stage centrifuge mounting arrangement;

FIG. 5 is a schematic view of a worm gear mounting arrangement;

FIG. 6 is a schematic view of a press set configuration;

FIG. 7 is a schematic view of a crush box construction;

shown in the figure: the device comprises a box body 1, a primary centrifuge 2, a secondary centrifuge 3, an extrusion group 4, a primary motor 5, a rotating rod 6, a primary main shaft 7, a fluted disc 8, a connecting column 9, a rotary disc 10, a secondary box body 11, a secondary main shaft 12, a worm wheel 13, a worm 14, a secondary motor 15, a main gear 16, an installation frame 17, a driven shaft 18, a driven gear 19, an extrusion box 20, a partition frame 21, a liquid bag 22, a cell disc 23 and a controller 24.

Detailed Description

The technical scheme of the invention is further explained by specific embodiments in the following with the accompanying drawings:

example one

Referring to the attached drawings 1-7, the invention aims to provide a mechanical compressive stress bionic stimulation device for stem cells, which realizes the functions of multidirectional force and linear pressurization. The multidirectional force and the linear pressurization are realized by the cooperation of double centrifugal devices, and the specific structure is as follows.

Whole device uses box 1 as installation main part 1, has one-level centrifuge 2 and second grade centrifuge 3 at box 1 internally mounted, and through the double-rotation cooperation of one-level centrifuge 2 and second grade centrifuge 3, the extrusion group 4 work of installation on the drive second grade centrifuge 3 realizes the multidirectional linear mechanics mechanical extrusion to cell dish 23.

As shown in fig. 3, the mounting structure of the primary centrifuge 2 is such that the primary centrifuge 2 adopts the cooperative rotation of the rotating rod 6 and the toothed disc 8. The rotary rod 6 is driven to rotate by a primary motor 5, and the primary motor 5 is mounted on the box body 1. Meanwhile, a rotatable primary main shaft 7 is installed on the box body 1, and a fluted disc 8 is installed through the primary main shaft 7. The teeth of the fluted disc 8 are horizontal teeth, and a connecting column 9 is fixedly arranged on the fluted disc 8. A rotary disc 10 is fixedly arranged on the connecting column 9, and a secondary centrifuge 3 is arranged on the rotary disc 10.

During the rotation of the rotating disk 10, the secondary centrifuge 3 is synchronously driven to rotate.

The structure of the secondary centrifuge 3 is shown in fig. 4, on which a secondary box 11 is mounted, and a matching rotating structure of a worm wheel 13 and a worm 14 is mounted in the secondary box 11. The worm 14 is driven to rotate by a secondary motor 15, and the worm wheel 13 is mounted in the secondary housing 11 via a secondary spindle 12. While a main gear 16 is mounted on the upper end of the secondary main shaft 12. A plurality of movable driven shafts 18 are arranged on the rotary table 10 outside the secondary box body 11. In order to ensure the stability of the driven shaft 18, the device is provided with a mounting frame 17 on the turntable 10 to assist in stabilizing the driven shaft 18.

A driven gear 19 on the driven shaft 18 is meshed with the main gear 16 to realize the rotation of the driven shaft 18. The driven shaft 18 is provided with a pressing group 4.

The structure of the extrusion group 4 is shown in fig. 6, and four groups of extrusion boxes are arranged inside the extrusion group. The squeeze box 20 is constructed as shown in FIG. 7, and the squeeze box 20 is divided into two spaces by a partition 21, in one of which a fluid bag 22 is placed and in the other of which a cell dish 23 is placed. The fluid bag 22 is made of soft material, and its shape can be changed during the centrifugation process. The liquid bag 22 generates multi-directional uneven and linear mechanical stimulation to the cell plate 23 through the action of centrifugal force.

Meanwhile, a controller 24 is arranged on the box body 1, and the rotation rates of the primary motor 5 and the secondary motor 15 are controlled through the controller 24, so that the centrifugal force is controlled.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. A bionic stimulation device for mechanical pressure stress of stem cells takes a box body as an installation main body, and is characterized in that a primary centrifuge is installed in the box body and drives a plurality of secondary centrifuges installed on the primary centrifuge to synchronously rotate, the secondary centrifuges drive an extrusion group installed on the secondary centrifuges to rotate, and a liquid bag in the extrusion group extrudes a cell tray through centrifugal force.

2. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 1, characterized in that: the first-stage centrifuge drives the fluted disc to rotate through the first-stage motor and the rotating rod.

3. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 2, characterized in that: the fluted disc is movably installed on the box body through the primary main shaft, and a connecting column and a turntable are arranged on the fluted disc.

4. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 3, characterized in that: and a secondary centrifuge is arranged on the rotary disc.

5. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 1, characterized in that: the secondary centrifuge takes a secondary box body as an installation main body, a worm wheel and worm matched rotating device is installed in the secondary box body, and the worm is driven by a secondary motor; the worm wheel is installed on the second grade main shaft, and second grade main shaft one end is mobilizable to be installed on the second grade box, and the master gear is installed to the other end, master gear and the driven gear intermeshing who installs on the driven shaft.

6. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 5, characterized in that: and the driven shaft is provided with an extrusion group.

7. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 5, characterized in that: the secondary main shaft is assisted and stabilized through a mounting frame.

8. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 1, characterized in that: a plurality of extrusion boxes are arranged in the extrusion group, and liquid bags and cell trays are arranged in the extrusion boxes.

9. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 8, characterized in that: the extrusion box is provided with a separation frame between the liquid bag and the cell tray.

10. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 1, characterized in that: and the box body is provided with a controller for controlling the primary motor and the secondary motor.