CN111040944A - Bioengineering cell culture is with shaking mixing device - Google Patents
Bioengineering cell culture is with shaking mixing device Download PDFInfo
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- CN111040944A CN111040944A CN201910252613.7A CN201910252613A CN111040944A CN 111040944 A CN111040944 A CN 111040944A CN 201910252613 A CN201910252613 A CN 201910252613A CN 111040944 A CN111040944 A CN 111040944A
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- 238000002156 mixing Methods 0.000 title claims abstract description 52
- 238000004113 cell culture Methods 0.000 title claims abstract description 34
- 230000003014 reinforcing effect Effects 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 230000001681 protective effect Effects 0.000 claims description 2
- 238000012360 testing method Methods 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 6
- 238000000034 method Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- Bioinformatics & Cheminformatics (AREA)
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- Chemical & Material Sciences (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a shaking and mixing device for bioengineering cell culture, which belongs to the technical field of biological equipment and comprises a fixed base, wherein a fixed groove is arranged in the middle of the fixed base, a driving shaft is arranged in the fixed groove, a driving wheel is arranged in the middle of the driving shaft, a swinging toothed frame is arranged outside the driving wheel, lower meshing teeth are arranged on the inner side of the lower end of the swinging toothed frame, upper meshing teeth are arranged on the inner side of the upper end of the swinging toothed frame, a swinging plate is arranged at the upper end of the swinging toothed frame, a fixed ring is arranged on the outer side of the upper end of the swinging plate, a rotating ring is arranged at the upper end of the fixed ring, a rotating shaft is arranged. According to the invention, the swinging plate is swung left and right, and the rotation of the mixing box is ensured through the matching between the rotating ring and the fixing ring, so that the vibrating and mixing effect of the mixing box on the cell culture test tube is realized.
Description
Technical Field
The invention relates to the technical field of biological equipment, in particular to a shaking and mixing device for bioengineering cell culture.
Background
In scientific research experiment and medical treatment application field, cell culture bottle and cell culture board are more general cell culture apparatus in earlier stage, and among technology and the clinical application, use the cell as carrier or object gradient experiment or contrast experiment, all can use a plurality of blake bottles or culture boards simultaneously, add different culture reagent according to the demand during cell culture, in order to make the cell evenly spread in blake bottle or culture board, need carry out the mixing and handle.
In the experiment of carrying out the medicine to cell growth influence, need carry out the mixing to the culture solution of cell growth environment the inside at interval, and make medicine and cell and culture solution and cell fully mix to prevent because of the time is longer, and make the material in medicine or the culture solution can not realize carrying out abundant contact to the cell, will lead to experimental data deviation. However, in the actual operation process, if manual blending is not performed, only natural diffusion blending is relied on, and sufficient blending is not easy, the accuracy of data is affected due to long time.
At present, in the process of cell culture, a culture bottle or a culture plate is manually shaken, a shaking table special for cell culture can only aim at cells cultured by the culture bottle, the purpose of uniform mixing is difficult to achieve, the experimental workload is increased, and the experimental time is prolonged.
Disclosure of Invention
The invention aims to provide a shaking and mixing device for bioengineering cell culture, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a bioengineering cell culture is with shaking mixing device, includes unable adjustment base, the unable adjustment base middle part is equipped with the fixed slot, and fixed slot internally mounted has the drive shaft, and the drive shaft mid-mounting has the drive wheel, and the drive wheel outside is equipped with swing tooth frame, and swing tooth frame lower extreme inboard is equipped with down the meshing tooth, and the upper end inboard is equipped with the meshing tooth, the wobble plate is installed to swing tooth frame upper end, and solid fixed ring is installed in the wobble plate upper end outside, and gu fixed ring installs rotatory ring in the fixed ring upper end, rotatory ring lower extreme mid-mounting has the rotation axis, and the rotating electrical machines is installed to the rotation axis lower extreme, rotatory ring installs the mixing case in the.
As a further scheme of the invention: and fixing legs are arranged on two sides of the lower end of the fixing base.
As a further scheme of the invention: and the output end of the rear side of the driving shaft is connected with a driving motor.
As a further scheme of the invention: the middle parts of the two ends of the fixing groove are provided with guide grooves, and guide springs are arranged inside the guide grooves.
As a further scheme of the invention: and a guide post is arranged at the other end of the guide spring and fixed in the middle of the two ends of the swing toothed frame.
As a further scheme of the invention: and ring teeth with a central angle of 180 degrees are arranged on the outer side of the driving wheel.
As a further scheme of the invention: the mixing tank bottom is inside to be equipped with the fender groove with spacing groove with quantity.
As a further scheme of the invention: the middle part of the upper end of the box cover is provided with a handle.
As a further scheme of the invention: and reinforcing rib plates are arranged between the two sides of the upper end of the swinging tooth frame and the swinging plate.
As a still further scheme of the invention: and a protective air bag is arranged in the middle of the lower end of the box cover.
Compared with the prior art, the invention has the beneficial effects that:
the utility model provides a bioengineering cell culture is with shaking mixing device, it is rational in infrastructure, the modern design through the drive wheel that is equipped with and mutually supporting between the swing tooth frame, realizes rocking about the swing board, simultaneously through the rotatory ring that is equipped with and the cooperation between the solid fixed ring, ensures the rotation of mixing case, and then has realized the mixing case and to the shaking mixing effect of cell culture test tube, and is effectual, the practicality is strong, the reliability is high.
Drawings
FIG. 1 is a schematic structural diagram of a shaking and mixing device for bioengineering cell culture.
FIG. 2 is a schematic structural diagram of a driving wheel in a shaking and mixing device for bioengineering cell culture.
Fig. 3 is a schematic sectional structure view of a-a in fig. 1.
In the figure: 1-fixed base, 2-fixed legs, 3-swinging toothed frame, 4-lower meshing teeth, 5-upper meshing teeth, 6-driving wheel, 7-driving shaft, 8-reinforcing plate, 9-swinging plate, 10-fixed ring, 11-rotating ring, 12-rotating motor, 13-rotating shaft, 14-blending box, 15-baffle groove, 16-limiting plate, 17-limiting groove, 18-handle, 19-box cover, 20-protective air bag, 21-guiding groove, 22-guiding column, 23-fixed groove, 24-guiding spring and 25-ring teeth.
Detailed Description
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.
Example 1
Referring to fig. 1 to 3, in the embodiment of the invention, a shaking and mixing device for bioengineering cell culture comprises a fixed base 1, wherein two sides of the lower end of the fixed base 1 are provided with fixed legs 2 for supporting and fixing the fixed base 1, the middle part of the fixed base 1 is provided with a fixed groove 23 for mounting and abdicating a swinging gear frame 3 and a driving wheel 6 to ensure that enough space is reserved between the swinging gear frame 3 and the driving wheel 6 for meshing, the middle parts of two ends of the fixed groove 23 are provided with guide grooves 21, a guide spring 24 is arranged in each guide groove 21, the other end of each guide spring 24 is provided with a guide column 22, each guide column 22 is fixed in the middle part of two ends of the swinging gear frame 3, the guide springs 24 and the guide columns 22 are matched with each other, so that the badon gear frame 3 does not shake in a large range in the process of moving left and, the output end of the rear side of the driving shaft 7 is connected with a driving motor for driving the driving shaft 7 to rotate so as to provide a power source for the rotation of the driving wheel 6, the driving wheel 6 is installed in the middle of the driving shaft 7, the outer side of the driving wheel 6 is provided with a ring tooth 25 with a central angle of 180 degrees, so that the ring tooth 25 can be respectively meshed with the upper meshing tooth 5 and the lower meshing tooth 4 in the rotating process, the outer side of the driving wheel 6 is provided with a swing tooth frame 3, the inner side of the lower end of the swing tooth frame 3 is provided with the lower meshing tooth 4, the inner side of the upper end is provided with the upper meshing tooth 5, the upper end of the swing tooth frame 3 is provided with a swing plate 9, the outer side of the upper end of the swing plate 9 is provided with a fixed ring 10, the upper end of the fixed ring 10 is provided with a rotating, be used for rotating for rotation axis 13 and providing the power source, mixing case 14 is installed to rotatory ring 11 upper end, mixing case 14 bottom is inside to be equipped with spacing groove 17 with the manger 15 of quantity, and then ensures the fixed to the cell culture test tube, and mixing case 14 inboard mid-mounting has limiting plate 16, and limiting plate 16 middle part is equipped with spacing groove 17, the detachable case lid 19 of installing in mixing case 14 upper end for the fixed mounting to mixing case 14 upper end is realized, 19 lower extreme mid-mounting of case lid has protection gasbag 20, is used for buffering to cell culture test tube upper end and supports, avoids the cell culture test tube to take place to rock at the during operation, 19 upper end mid-mounting of case lid has handle 18 for make things convenient for the staff to carry out the dismantlement installation to.
Example 2
In another embodiment of the present invention, the difference between this embodiment and the above embodiment is that a reinforcing rib plate 8 is installed between both sides of the upper end of the swing tooth frame 3 and the swing plate 9, and the fixed installation between the swing tooth frame 3 and the swing plate 9 is further increased.
When the test tube mixing box is used, a test tube for cell culture is fixed in the limiting groove 17 in the middle of the limiting plate 16, the box cover 19 is closed, the driving motor is started, the driving shaft 7 is driven by the driving motor to rotate, the ring teeth 25 on the outer side of the driving wheel 6 are further driven to rotate synchronously, the ring teeth 25 can be alternately meshed with the upper meshing teeth 5 and the lower meshing teeth 4 in the rotating process, the swinging plate 9 can swing left and right, on the other hand, the rotating motor 12 drives the rotating shaft 13 to rotate, then the mixing box 14 is driven to rotate, the test tube in the mixing box 14 can also rotate while swinging left and right, the mixing effect is greatly increased, and the mixing effect is good. The cell culture tube shaking device is reasonable in structure and novel in design, the driving wheel 6 and the swinging toothed frame 3 are matched with each other, the swinging plate 9 is shaken leftwards and rightwards, meanwhile, the rotating ring 11 and the fixing ring 10 are matched with each other, the rotation of the mixing box 14 is ensured, the shaking and mixing effect of the mixing box 14 on cell culture tubes is further realized, the effect is good, the practicability is high, and the reliability is high.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. The utility model provides a bioengineering cell culture is with shaking mixing device, includes unable adjustment base (1), unable adjustment base (1) middle part is equipped with fixed slot (23), and fixed slot (23) internally mounted has drive shaft (7), and drive shaft (7) mid-mounting has drive wheel (6), its characterized in that, and the drive wheel (6) outside is equipped with swing tooth frame (3), and swing tooth frame (3) lower extreme inboard is equipped with lower meshing tooth (4), and the upper end inboard is equipped with upper meshing tooth (5), swing tooth frame (3) upper end is installed swinging plate (9), and the outer side of swinging plate (9) upper end is installed solid fixed ring (10), and gu fixed ring (10) upper end is installed rotatory ring (11), and rotatory ring (11) lower extreme mid-mounting has rotation axis (13), and rotation motor (12) are installed to rotation axis (13) lower extreme, blending case, a limiting plate (16) is installed in the middle of the inner side of the mixing box (14), a limiting groove (17) is formed in the middle of the limiting plate (16), and a box cover (19) is detachably installed at the upper end of the mixing box (14).
2. The shaking and mixing device for bioengineering cell culture according to claim 1, wherein the fixing legs (2) are installed on both sides of the lower end of the fixing base (1).
3. The shaking and mixing device for bioengineering cell culture according to claim 1, wherein the output end of the rear side of the driving shaft (7) is connected with a driving motor.
4. The shaking and mixing device for bioengineering cell culture according to claim 1, wherein the middle parts of the two ends of the fixing groove (23) are provided with guide grooves (21), and guide springs (24) are arranged in the guide grooves (21).
5. The shaking and mixing device for bioengineering cell culture according to claim 4, wherein the guide post (22) is installed at the other end of the guide spring (24), and the guide post (22) is fixed in the middle of the two ends of the swing toothed frame (3).
6. The shaking and mixing device for bioengineering cell culture according to claim 1, wherein the driving wheel (6) is provided with ring teeth (25) with a central angle of 180 degrees on the outer side.
7. The shaking and mixing device for bioengineering cell culture according to claim 1, wherein the bottom of the mixing box (14) is provided with retaining grooves (15) in the same number as the limiting grooves (17).
8. The shaking and mixing device for bioengineering cell culture according to claim 1, wherein the middle part of the upper end of the box cover (19) is provided with a handle (18).
9. The shaking and mixing device for bioengineering cell culture according to claim 1, wherein a reinforcing rib plate (8) is installed between the two sides of the upper end of the swing tooth frame (3) and the swing plate (9).
10. The shaking and mixing device for bioengineering cell culture according to claim 1 or 8, wherein the middle part of the lower end of the box cover (19) is provided with a protective air bag (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910252613.7A CN111040944A (en) | 2019-03-29 | 2019-03-29 | Bioengineering cell culture is with shaking mixing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910252613.7A CN111040944A (en) | 2019-03-29 | 2019-03-29 | Bioengineering cell culture is with shaking mixing device |
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| Publication Number | Publication Date |
|---|---|
| CN111040944A true CN111040944A (en) | 2020-04-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910252613.7A Pending CN111040944A (en) | 2019-03-29 | 2019-03-29 | Bioengineering cell culture is with shaking mixing device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112608846A (en) * | 2021-01-15 | 2021-04-06 | 吉林大学中日联谊医院 | Multifunctional cell culture device for improving premature ovarian failure stem cell exosomes |
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2019
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Application publication date: 20200421 |
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