CN210012859U - Multi-channel differential traction device for research on in-vitro axon stress mechanical response mechanism - Google Patents
Multi-channel differential traction device for research on in-vitro axon stress mechanical response mechanism Download PDFInfo
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- CN210012859U CN210012859U CN201920020991.8U CN201920020991U CN210012859U CN 210012859 U CN210012859 U CN 210012859U CN 201920020991 U CN201920020991 U CN 201920020991U CN 210012859 U CN210012859 U CN 210012859U
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Abstract
The utility model provides a multi-channel differential traction device for researching an in-vitro axon stress mechanical response mechanism, which comprises a culture and traction control system and a mechanical device, wherein the culture and traction control system comprises a cell culture box, an upper computer, a controller and a driving motor; the mechanical device comprises a first coupler, a gear shaft seat, a second coupler, a first lead screw nut linear sliding table, a second lead screw nut linear sliding table, a cell traction growth device, a device support frame and a base; the screw rotating directions of the first screw nut linear sliding table and the second screw nut linear sliding table are different. The device can be suitable for realizing nerve axon traction growth experiments with multiple sample numbers, and can provide flexible test traction speed.
Description
Technical Field
The utility model relates to a biomedical experiment field, in particular to a multichannel differential tractive device that is used for external axon response mechanics mechanism to study.
Background
With the development of economic levels of countries in the world, the incidence of spinal cord injury tends to increase year by year. In developed countries, the incidence of spinal cord injury is approximately 13.3-45.9 persons/million persons/year. In china, about 6 million patients with spinal cord injuries are newly increased each year. At present, the clinical treatment method for spinal cord injury mainly comprises operations, drug therapy, long-term exercise rehabilitation and the like. However, the massive loss of neural tissue and the failure of regenerative function make current treatment approaches very limited.
At present, the clinical nerve repair operation mainly comprises autologous nerve transplantation and cell transplantation, and can promote the regeneration, repair and remodeling of damaged or damaged nerves, reconstruct nerve anatomical projection passages and loops, regulate and improve nerve signal conduction and finally realize nerve function repair. However, there are corresponding concerns with each of the two methods: the autologous nerves cannot meet the requirement of repairing a large number of damaged nerves due to limited sources, small diameters and the like, and the intercepted nerves can permanently lose functions and have the risk of forming neuroma; cells are transplanted through a damage target point way, so that local brain tissues and spinal cord tissues can be damaged, the damage is heavier during multi-target point transplantation, the transplantation through the blood way is greatly influenced by blood components and in-vivo metabolic factors and is also influenced by a blood brain barrier, the cells are transported through cerebrospinal fluid, are attached to the surface of a soft membrane at a damaged part, and can permeate into nerve roots and brain tissues widely.
Chinese patent 201410403385.6 discloses a nerve axon drawing and growing device, which comprises a culture and drawing control system and a mechanical device. The controller is connected with and drives the stepping motor to rotate, the ball screw linear sliding table at one end of the coupler is driven to displace, the cell traction growth device is fixed on the device supporting frame, and the nerve axon is indirectly pulled through the traction connecting block fixed on the linear sliding table. However, only two groups of traction devices can be connected in the device, the speeds of the traction blocks are consistent, the quantity of nerve samples is insufficient, the influence of the traction speed on the growth of the axon of the nerve cannot be well tested, and in addition, the stepping traction acceleration is large, so that the nerve is easily damaged.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a can be suitable for realizing the nerve axon tractive growth device of many sample counts to can provide nimble test tractive speed.
The technical scheme of the utility model as follows.
A nerve axon pulling growth device comprising a culture and pulling control system and a mechanical device, wherein:
the culture and traction control system comprises a cell culture box, an upper computer, a controller and a driving motor;
the mechanical device comprises a first coupler, a gear shaft seat, a second coupler, a first lead screw nut linear sliding table, a second lead screw nut linear sliding table, a cell traction growth device, a device support frame and a base; the screw rotating directions of the first screw nut linear sliding table and the second screw nut linear sliding table are different.
Preferably, the cell culture box is provided with a pore channel communicated with the outside and used for being connected with the controller through a data line of the driving motor.
Preferably, the pore is located at the rear side of the cell culture box, and the joint of the pore and the data line is sealed by silica gel.
Preferably, the host computer is capable of controlling parameters of the cell pulling process, including one or more of displacement, speed, duration of cell pulling.
Preferably, a plurality of gear shafts are respectively corresponding to each of the first screw nut linear sliding table and the second screw nut linear sliding table; the first coupling connects the driving motor with one of the gear shafts; and each gear shaft is connected with the corresponding linear sliding table of the screw nut by the second coupler.
Preferably, the first screw nut linear sliding table and the second screw nut linear sliding table are respectively provided with a plurality of linear sliding tables which are arranged alternately, and corresponding gear shafts of two adjacent screw nut sliding tables are meshed with each other.
Preferably, each lead screw of the first lead screw nut linear sliding table and the second lead screw nut linear sliding table has different screw pitches, and therefore the respective nuts can generate different displacements.
Preferably, the controller can receive an instruction sent by the upper computer, so as to drive the driving motor to rotate; the driving motor can drive a plurality of gear shafts to rotate, and then drives the screw nut linear sliding table to generate linear displacement.
Preferably, a plurality of cell drawing and growing devices are provided, and correspond to each of the first screw nut linear sliding table and the second screw nut linear sliding table respectively; the cell traction growth device is arranged on the device support frame and is fixed on a base together with the first and second screw nut linear sliding tables and the gear shaft seat, and the base is placed in a cell culture box;
each cell traction growth device comprises a culture seat, a cover, an integrated traction piece, a traction film and a bottom film; the integrated traction block and the rod can be driven by the nut of the screw nut linear sliding table to cause the movement of the traction film, so that the nerve axon is pulled.
Preferably, the culture seat is of an integrated rectangular structure; the integrated pulling piece comprises a pulling block part and a pulling rod part; the traction block part is positioned in the middle of the culture seat, and the traction rod part is positioned in the center of the end part of the culture seat; the bottom film is adhered to the bottom groove of the culture seat; the cover is arranged on the grooves on the two sides and the upper surface of the culture seat and is fixed.
The utility model has the advantages that:
(1) the utility model discloses a multichannel, the neural axon draw gear of differential have been constructed, can be under same cultivation environment a plurality of independent draw gear of simultaneous control to realize length, the speed of the axon growth of arithmetic progression formula, convenient experiment contrast, both can verify the feasibility of the amazing nerve growth of mechanical tractive, also can test the influence of tractive speed to the nerve axon growth.
(2) The utility model discloses can gather tractive stress data through force sensor, the analysis stress size is to the influence of nerve axon growth, establishes out the external best condition of cultivating the perfect neural tissue of function for the nerve damage is restoreed, and carries out neural restoration for realizing transplanting the tractive device in vivo and establishes the technical key.
Drawings
Fig. 1 is a schematic structural view of a nerve axon drawing and growing device of the present invention;
FIG. 2 is a mechanical structure diagram of the cell growth device of the present invention;
FIG. 3 is a top view of the cell-pulling and growing device without a cover according to the present invention;
FIG. 4 is a side view of the integrated pulling block and rod of the present invention;
FIG. 5 is a drawing schematic diagram of the cell drawing and growing device of the present invention;
figure 6 is a diagram of the structure of axonal traction growth control of the present invention.
Detailed Description
The technical solution in the embodiments of the present invention will be described in detail and clearly with reference to the accompanying drawings in the embodiments of the present invention. The specific embodiments described herein are merely illustrative of the invention and do not limit the invention.
As shown in figure 1, the utility model relates to a multi-channel, differential traction device for the research of the in vitro axon stress mechanical response mechanism, which mainly comprises a culture and traction control system and a mechanical device. The culture and traction control system comprises a cell culture box 1, an upper computer 2, a controller 3 and a direct current motor 4, and the mechanical device comprises a first coupler 5 connected with the direct current motor, a gear shaft 6, a second coupler 7 connected with the gear shaft, a left-handed lead screw nut linear sliding table 8, a right-handed lead screw nut linear sliding table 9, a cell traction growth device 10, a device support frame 11 and a base 12.
The cell culture case 1 rear side is equipped with a communicating pore with the external world, DC motor 4's data line passes through pore connection controller 3, and pore and data line junction adopt silica gel sealing. The cell culture box 1 is a sealed structure and can provide carbon dioxide, temperature and humidity environment required by cell growth. In a preferred embodiment, the cell culture chamber 1 is configured to ensure culture conditions of 37 ℃ temperature, 95% relative humidity and 5% carbon dioxide, which provide an optimal carbon dioxide, temperature and humidity environment for cell growth.
The upper computer 2 can control parameters of the traction process including cell traction speed, displacement and duration through programming. The controller 3 receives a control instruction of the upper computer 2, so that the direct current motor 4 is driven to rotate, the direct current motor is adopted for stable and continuous output, the stepping motor can generate overlarge instantaneous acceleration when rotating every time, and the axon is easy to break due to overlarge pulling force.
The left-handed screw nut linear sliding table 8 and the right-handed screw nut linear sliding table 9 are respectively provided with a plurality of sliding tables, and preferably have equal numbers. The cell traction and growth device is characterized in that the first coupler 5 and the gear shaft 6 which are connected with the direct current motor, the second coupler 7 and the cell traction and growth device 10 which are connected with the gear shaft are respectively multiple and respectively correspond to each of the left-handed lead screw nut linear sliding table 8 and the right-handed lead screw nut linear sliding table 9.
The direct current motor 4 drives one of the gear shafts 6 connected with one end of the first coupler 5 of the direct current motor to rotate, and each gear shaft 6 drives the left-handed lead screw nut linear sliding table 8 and the right-handed lead screw nut linear sliding table 9 connected with one end of the second coupler 7 of the gear shaft to generate displacement respectively. Due to the transmission between the gear shafts, the steering directions of two adjacent gear shafts are different, so that screw rods with different screwing directions are designed. Because the thread pitch of each lead screw is different, the nut is displaced differently, so that the nerve axon is indirectly pulled through the integrated pulling block fixed on the linear slipway nut and the rod 14, and the displacement and the speed of the pulling are different.
In order to prevent the axon from breaking due to excessive mechanical pulling displacement, the pulling time is 4-5 minutes per day, and the pulling displacement is about 0.5 mm. Accordingly, in a preferred embodiment, for the left-handed screw nut linear sliding table 8 and/or the right-handed screw nut linear sliding table 9, a screw with a thread pitch of 0.5mm can be selected according to the specification of fine threads, and the rotation speed of the motor is set to 1 revolution in 5 minutes. The lead screw can meet the requirement of axon traction machinery traction, and can ensure that the whole mechanical device is easy to process and manufacture.
In a preferred embodiment, the thread pitch of the individual threaded spindles, which comprise different directions of rotation for the left and right hand, is arranged in an arithmetic progression, whereby the displacement of the individual threaded spindles is correspondingly also arithmetic progression. In a more preferred embodiment, the displacement of each nut is set to be 1, 2, 3, 4 times the minimum pitch, so that the displacement and the speed of the corresponding cell growth device 10 are correspondingly related.
The left-handed screw nut linear sliding table 8 and the right-handed screw nut linear sliding table 9 are provided with through holes in the middles of nuts, and threaded holes in the top ends of the nuts, and the nuts and the integrated traction block are fixed to a rod.
As shown in fig. 2 to 5, the cell growth device 10 is a rectangular parallelepiped structure, and is composed of a culture base 13, an integrated pulling block and rod 14, a supporting block 15, a cover 16, a bottom membrane 17, and a pulling membrane 18.
Wherein, the culture seat 13 is an integrated design structure. The supporting block 15 is located on the left side of the culture seat 13, two ends of the supporting block are fastened by screws, and the integrated pulling block and the rod 14 are located on a groove in the middle of the culture seat 13 and can move in the groove of the culture seat 13. The bottom film 17 is adhered to the bottom small groove of the culture seat 13 by coating silica gel for cell culture. For the convenience of viewing, the cover 16 is made of a transparent material and is fastened with screws.
The middle through hole of the supporting block 15 can support the integrated pulling block and the rod 14 and can realize the guiding function of the movement of the pulling block and the rod. The top of the supporting block 15 is provided with three screw through holes, the upper screw through hole and the lower screw through hole are used for fixing the integrated pulling block and the rod 14, and the integrated pulling block and the rod 14 are prevented from loosening in the cell culture device.
Wherein, the left side of the integrated pulling block and rod 14 is a pulling rod part, and the right side is a pulling block part. The traction rod part penetrates through a middle through hole of the supporting block 15, extends out of the culture seat 13, penetrates through nuts of the lead screw nut linear sliding tables 8 and 9, and is fastened by screws, so that the two parts are integrated. The T-shaped frame on the upper part of the drawing block part is arranged on the middle groove of the culture seat 13, and the arc shape on the lower part of the drawing block part is used for drawing the drawing film 18, so that the extending part of the drawing film is attached to the bottom film 17.
The pulling film 18 is a rectangular transparent film and is vertically adhered to the pulling block portion of the integrated pulling block and rod 14 by silica gel. As shown in FIG. 5, the extension part of the traction film 18 is closely attached to the bottom film 17, and mechanical stimulation can be generated to axons growing on the traction film 18 and the bottom film 17 through mechanical traction.
The culture seat 13 and the supporting block 15 of the cell traction growth device 10 are both made of polytetrafluoroethylene materials, the cover 16 is made of organic glass materials, and the integrated traction block and the rod 14 are made of stainless steel materials.
In this embodiment, a set of nerve axon stretching and growing device is designed for stretching and growing nerve axons.
When in use, the pulling film 18 and the bottom film 17 are assembled. After the device is assembled, the device is placed under an ultraviolet lamp for several hours until the silica gel is solidified, and then sterile water is added for soaking for several days until the release of the silica gel acetic acid is finished, so that cell culture can be carried out. The nerve cells or tissues are placed at two sides of the traction membrane 18 and the bottom membrane 17 within 100 microns of each other, and mechanical traction can be carried out on the axons when the nerve cells at the two sides form synaptic connections.
In this example, as shown in fig. 6, a control instruction of the upper computer 2 is downloaded to the controller 3 through a data line, so as to drive the dc motor 4 to rotate, and further drive the gear shaft to rotate through the first coupler 5, and then drive the nuts of the screw nut linear sliding tables 8 and 9 to displace through the second coupler 7, and further drive the integrated pulling block and the rod 14 fixedly connected with the nuts to move, and finally the integrated pulling block and the rod 14 drive the pulling membrane 18 to move on the bottom membrane 17, so as to pull the nerve axons growing on the pulling membrane 18 and the bottom membrane 17.
In this embodiment, the drawing film 18 and the bottom film 17 are polychlorotrifluoroethylene films, which have good biocompatibility, transparency, high temperature and high pressure resistance, are not easily deformed, and are convenient for sterilization, observation and drawing. Preferably, polychlorotrifluoroethylene films of 50 μm and 198 μm are used for the pulling film 18 and the bottom film 19, respectively.
In this embodiment, the four independent cell growth devices 10 are pulled at the same time, so that the pulling speeds and displacements of the four independent cell growth devices 10 are different. Further, the number of the cell traction growth devices 10 can be increased according to experimental requirements, the number of the gear shaft 6, the second coupling 7, the left-handed screw nut linear sliding table 8 and the right-handed screw nut linear sliding table 9 is increased, and more diversified traction speeds and displacements of the cell traction growth devices 10 are realized.
It can be understood by those skilled in the art that, although the cell pulling and growing device and the screw nut linear slide table are 4 sets in the present embodiment, the present invention is not limited thereto. The cell drawing and growing device and the number of the lead screw nut linear sliding tables can be set according to the number of samples required actually, and in a preferred embodiment, the left-handed lead screw nut linear sliding table and the right-handed lead screw nut linear sliding table are set to be the same in number; in a more preferred embodiment, the number of the cell pulling growth devices is set to be a multiple of 4.
In an alternative embodiment (not shown), the traction membrane 18 and the base membrane 17 may be plated with electrode contacts for connection to a multichannel neural signal recording stimulation system for recording neural signals and selectively stimulating different sites of the nerve. A film force sensor may be attached to the stretch film 18 for recording the stress of the stretch.
In another alternative embodiment, a set of electrical stimulation protocols for nerve axons is provided, and the bottom film 17 and the pulling film 18 are flexible circuit boards of a transparent microelectrode array. After the nerve axon is pulled and grown, the pulled and grown axon can be electrically stimulated, and corresponding nerve electrophysiological signals are recorded.
Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Claims (10)
1. A multi-channel differential traction device for researching an in-vitro axon strain mechanical response mechanism comprises a culture and traction control system and a mechanical device, wherein:
the culture and traction control system comprises a cell culture box, an upper computer, a controller and a driving motor;
the mechanical device comprises a first coupler, a gear shaft seat, a second coupler, a first lead screw nut linear sliding table, a second lead screw nut linear sliding table, a cell traction growth device, a device support frame and a base;
the screw rotating direction of the first screw nut linear sliding table is different from that of the second screw nut linear sliding table.
2. The multi-channel differential traction device for researching the in-vitro axon strain mechanical response mechanism according to claim 1, wherein the cell culture box is provided with a duct communicated with the outside for connecting with a controller through a data line of the driving motor.
3. The multi-channel differential drawing device for researching the in-vitro axon strain mechanical response mechanism according to claim 2, wherein the pore channel is positioned at the rear side of the cell culture box, and the joint of the pore channel and the data line is sealed by silica gel.
4. The multi-channel differential traction device for in vitro axonal stress mechanical response mechanism study according to claim 1, wherein the upper computer can control parameters of the traction process, including one or more of displacement, speed, duration of cell traction.
5. The multi-channel differential traction device for in vitro axonomethric stress response mechanism research according to claim 1, characterized in that the gear shaft is provided with a plurality of gear shafts, which correspond to each of the first screw nut linear sliding table and the second screw nut linear sliding table respectively; the first coupling connects the driving motor with one of the gear shafts; and each gear shaft is connected with the corresponding linear sliding table of the screw nut by the second coupler.
6. The multi-channel differential traction device for in vitro axon stress mechanical response mechanism research of claim 5, wherein the first lead screw nut linear sliding table and the second lead screw nut linear sliding table are respectively provided with a plurality of linear sliding tables and are arranged alternately, and corresponding gear shafts of two adjacent lead screw nut sliding tables are meshed with each other.
7. The multi-channel differential traction device for in vitro axonal stress mechanical response mechanism study according to claim 6, characterized in that each lead screw of the first and second lead screw nut linear slides has a different pitch, whereby each nut can generate different displacements.
8. The multi-channel differential traction device for researching the in-vitro axon strain mechanical response mechanism according to claim 5, wherein the controller is capable of receiving a command sent by the upper computer so as to drive the driving motor to rotate; the driving motor can drive a plurality of gear shafts to rotate, and then drives the screw nut linear sliding table to generate linear displacement.
9. The multi-channel differential traction device for in vitro axonal stress response mechanism research according to any one of claims 1-8, wherein the cell traction and growth device has a plurality of cells, corresponding to each of the first and second lead screw nut linear slides, respectively; the cell traction growth device is arranged on the device support frame and is fixed on a base together with the first and second screw nut linear sliding tables and the gear shaft seat, and the base is placed in a cell culture box;
each cell traction growth device comprises a culture seat, a cover, an integrated traction piece, a traction film and a bottom film; the integrated traction block and the rod can be driven by the nut of the screw nut linear sliding table to cause the movement of the traction film, so that the nerve axon is pulled.
10. The multi-channel differential traction device for researching the in-vitro axon-mechanical response mechanism according to claim 9, wherein the culture seat is of an integrated rectangular structure; the integrated pulling piece comprises a pulling block part and a pulling rod part; the traction block part is positioned in the middle of the culture seat, and the traction rod part is positioned in the center of the end part of the culture seat; the bottom film is adhered to the bottom groove of the culture seat; the cover is arranged on the grooves on the two sides and the upper surface of the culture seat and is fixed.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822083734 | 2018-12-12 | ||
| CN2018220837347 | 2018-12-12 |
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| CN210012859U true CN210012859U (en) | 2020-02-04 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628305A (en) * | 2018-12-12 | 2019-04-16 | 北京理工大学 | The multichannel differential pulling device for answering mechanics response mechanism to study for external aixs cylinder |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628305A (en) * | 2018-12-12 | 2019-04-16 | 北京理工大学 | The multichannel differential pulling device for answering mechanics response mechanism to study for external aixs cylinder |
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Granted publication date: 20200204 Termination date: 20210107 |