CN101799495A - Automatic impedance-testing system for flexible film microelectrode arrays - Google Patents
Automatic impedance-testing system for flexible film microelectrode arrays Download PDFInfo
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- CN101799495A CN101799495A CN 201010132952 CN201010132952A CN101799495A CN 101799495 A CN101799495 A CN 101799495A CN 201010132952 CN201010132952 CN 201010132952 CN 201010132952 A CN201010132952 A CN 201010132952A CN 101799495 A CN101799495 A CN 101799495A
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Abstract
The invention provides an automatic impedance-testing system for flexible film microelectrode arrays in the technical field of biomedicine. The system comprises an electrode connection device, a multi-path choice circuit, a computer and an LCR analyzer, wherein one end of the electrode connection device is connected with the multi-path choice circuit to transmit control word information, and the other end of the electrode connection device is connected with a to-be-tested electrode to transmit test data information; the multi-path choice circuit is connected with the LCR analyzer to transmit the test data information; the LCR analyzer is connected with the to-be-tested electrode to transmit the test data information; the computer is connected with the multi-path choice circuit to transmit the control word information; and the computer is connected with the LCR analyzer to transmit the test data information. The system has the advantages of improving test efficiency, reducing test errors and damage to electrodes and applying to film electrodes in different size and arrangement within a certain range, along with channel scalability, friendly human-machine interface and capability of programming according to different test requirements so as to provide operation convenience for testers.
Description
Technical field
What the present invention relates to is a kind of test macro of field of biomedicine technology, specifically is a kind of automatic impedance-testing system of flexible film microelectrode arrays.
Background technology
Vision prosthesis be a kind of can be with Image Information Processing, coding, by microelectrode array to the visual system effect, thereby produce pseudophotoesthesia in visual centre, recover the man-made organ of blind person's eyesight.According to the difference of stimulation sites, vision prosthesis can be divided into visual cortex prosthese, optic nerve prosthese and retina prosthese basically, and wherein, the retina prosthese can be divided on the retina prosthese under the prosthese and retina again.The retina prosthese is the research direction of relatively being paid close attention in recent years, and clinical trial is carried out at most, and is hopeful most to put goods on the market early.
The stimulating electrode of retina prosthese is for implanting eyeball inside, being fitted in the flexible film microelectrode on the retina.In recent years, because the fast development of MEMS (micro electro mechanical system) (MEMS) and electrochemical techniques, the manufacture craft of flexible film microelectrode has obtained breakthrough progress---on the material, adopt biocompatibility preferably polyimide or Parylene as flexible substrate, with the highdensity corrosion resistant metal making of Low ESRs such as gold and platinum stimulating electrode; Quantitatively, developed into 25,60 from 16 initial electrod-arrays, even more than 1000; On the size, electrode diameter has developed into micron order from the millimeter level; On arranging, develop to bilayer from individual layer, or the like.In order to obtain to have the visually-perceptible of high-resolution, just need make stimulating electrode with the contact area of retinal tissue on as much as possible little, and has higher charge transport ability when having lower impedance, so before the experiment of the clinical physiological that encapsulates and implant, the test of electrod-array impedance is even more important.
Traditional testing impedance adopts three-electrode method, and device therefor is generally LCR (accurate electric impedance analyzer) analyser, needs the contact point of manual conversion probe and membrane electrode to select single tested electrode during test.Because the area in electrode stimulating site often has only hundreds of or tens microns, it is tight to arrange in limited zone, backing material is very thin and soft simultaneously, so manual test is inefficiency often, and very easily in test process, damage membrane electrode, influence test result, cause unnecessary waste.
Through existing literature search is found, one piece of document (Kindlundh M of Sweden, Norlin P.A gel-basedwafer-level testing procedure for microelectrodes[J] .Sensors and Actuators B Chem, 2005,107 (2): 557-562.) mentioned the wafer-level test system of chip microelectrode impedance, this technology comprises utilizes a kind of probe (Probe Card) and multiplex adapter (Multiplexer) to select tested microelectrode, to improve testing efficiency and accuracy rate.But, in the field of retina prosthese flexible film microelectrode, also do not find and the closely-related automatic impedance-testing system of the present invention; In addition, existing probe bed proving installation is mainly used in the detection of printed circuit board (PCB), and its measure of precision can not directly apply to the test of film microelectrode impedance.
Summary of the invention
The objective of the invention is to overcome the above-mentioned deficiency of prior art, a kind of automatic impedance-testing system of flexible film microelectrode arrays is provided.The present invention can select tested membrane electrode automatically, and testing efficiency height and test error are little, and simultaneously concrete extensibility can be applicable to the membrane electrode of arranging.
The present invention is achieved by the following technical solutions:
The present invention includes: electrode connection device, multiplexer circuit, computing machine and LCR analyser, wherein: an end of electrode connection device links to each other with multiplexer circuit and transmits control word information, the other end of the electrode connection device transmitting test data information that links to each other with electrode to be measured, the multiplexer circuit transmitting test data information that links to each other with the LCR analyser, the LCR analyser transmitting test data information that links to each other with electrode to be measured, the computing machine transmission control word information that links to each other with multiplexer circuit, the computing machine transmitting test data information that links to each other with the LCR analyser.
Described electrode connection device comprises: four dog screws, probe circuit plate and base plates, and wherein: dog screw is positioned at the endpoint location of probe circuit plate and base plate, and probe circuit plate and base plate are fixedly linked by dog screw.
Described dog screw is provided with spring.
Described probe circuit plate comprises: circuit board, probe array, observation panel and connector, wherein: circuit board is fixed on the base plate, and probe array is welded on the circuit board, and observation panel is arranged on a side of circuit board, connector is arranged on the circuit board, and connector links to each other with multiplexer circuit.
Described probe array comprises the spring probe of some round ends, and the diameter of this probe is less than tested membrane electrode pad, and the spread pattern of probe array is identical with the spread pattern of tested membrane electrode pad.
Described base plate is provided with the marginal portion of two anchor clamps with fixing tested membrane electrode.
Described small fixture is a packaged type, to prevent to damage tested membrane electrode.
Described multiplexer circuit comprises: microcontroller and signal processor, wherein: microcontroller links to each other with computing machine and transmits control word information, the input end of the signal processor transmitting test data information that links to each other with the LCR analyser, the output terminal of the signal processor transmitting test data information that links to each other with electrode connection device, the microcontroller transmitting test data information that links to each other with signal processor.
Principle of work of the present invention: the pad portion of tested membrane electrode is fixed on the electrode connection device, the corresponding contact in pad site with probe array, the common immersion of the stimulation site part of electrode and the Pt electrode of LCR analyser and Ag/AgCl contrast electrode fills in the beaker of physiological saline, electrode connection device connects multiplexer circuit, the control word of multiplexer circuit receiving computer to be being assigned to the target output terminal to the LCR test signal, thereby reaches the purpose of automatic conversion test access.
Compared with prior art, the invention has the beneficial effects as follows: replaced the impedance test device that relies on the manual change interface channel originally, can improve testing efficiency, reduce test error and the damage of electrode; Channelled extensibility can be applicable to the membrane electrode of arranging; Man-machine interface close friend, and can programme according to different test requests, make things convenient for the tester to operate.
Description of drawings
Fig. 1 is that system of the present invention forms synoptic diagram.
Fig. 2 is the structural representation of electrode connection device and tested membrane electrode;
Wherein: (a) be the structural representation of electrode connection device; (b) be the structural representation of tested membrane electrode.
Fig. 3 is the composition synoptic diagram of multiplexer circuit.
Fig. 4 is the auto test flow figure of embodiment.
Embodiment
Below in conjunction with accompanying drawing system of the present invention is further described: present embodiment is being to implement under the prerequisite with the technical solution of the present invention, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment
As shown in Figure 1, present embodiment comprises: electrode connection device, multiplexer circuit, computing machine and LCR analyser, wherein: an end of electrode connection device links to each other with multiplexer circuit and transmits control word information, the other end of the electrode connection device transmitting test data information that links to each other with electrode to be measured, the multiplexer circuit transmitting test data information that links to each other with the LCR analyser, the LCR analyser transmitting test data information that links to each other with electrode to be measured, computing machine links to each other with multiplexer circuit by the RS232 serial ports and transmits control word information, computing machine is by the USB/LAN/GPIB port transmitting test data information that links to each other with the LCR analyser, the pad portion of membrane electrode to be measured is fixed in electrode connection device, the stimulation site part of membrane electrode to be measured and the Pt electrode of LCR analyser, the common immersion of Ag/AgCl contrast electrode fills in the beaker of physiological saline.
The structural representation of membrane electrode to be measured is shown in Fig. 2 (b).
Shown in Fig. 2 (a), described electrode connection device comprises: four dog screws, probe circuit plate and base plates, wherein: the size of probe circuit plate and base plate is 6cm * 6cm～10cm * 10cm, the material of base plate is a printed circuit board (PCB), dog screw is positioned at the endpoint location of probe circuit plate and base plate, and probe circuit plate and base plate are fixedly linked by dog screw.
Described probe circuit plate comprises: circuit board, probe array, observation panel and connector, wherein: the material of circuit board is a printed circuit board (PCB), circuit board is fixed on the base plate, probe array is welded on the circuit board, observation panel is arranged on a side of circuit board, connector is arranged on the circuit board, and the probe circuit plate links to each other with multiplexer circuit by connector.
Described observation panel size is 1cm * 2cm, and it is convenient to observe and regulate the position of tested membrane electrode at base plate.
Described probe array comprises the spring probe of some round ends, the diameter of this probe is less than tested membrane electrode pad, and the spread pattern of probe array is identical with the spread pattern of tested membrane electrode pad, and the number of probe is identical with the number of tested membrane electrode pad.
Described base plate is provided with the marginal portion of two small fixture with fixing tested membrane electrode.
Described small fixture is the silica gel encapsulation, to prevent to damage tested membrane electrode.
Described dog screw is provided with spring, and the dynamics of pushing by regulating spring can guarantee the effective contact of probe array to tested membrane electrode pad.
As shown in Figure 3, described multiplexer circuit comprises: microcontroller and two signal processors, wherein: microcontroller links to each other with computing machine and transmits control word information, the input end of the signal processor transmitting test data information that links to each other with the LCR analyser, the output terminal of the signal processor transmitting test data information that links to each other with the connector of electrode connection device, the microcontroller transmitting test data information that links to each other with signal processor.
Microcontroller is selected the AVR single-chip microcomputer of ATmega series RISC framework for use in the present embodiment, and its characteristics are: have advanced instruction set and one-cycle instruction execution time.Computing machine writes control word by the RS232 serial ports to the AVR single-chip microcomputer, the I/O mouth of AVR single-chip microcomputer links to each other with data input port (DIN) with the Enable Pin (SYNC) of signal selector respectively with main frame output/slave input port (MOSI), the LCR test signal of signal selector input end is sent to selected output port, realize the purpose that test channel is selected automatically.
Signal selector adopts ADG731 in the present embodiment, and its internal driving is little; 32 of output ports can be expanded at double, to satisfy the requirement of tested membrane electrode number.
Described computer operation interface can be opened programming, to realize different test patterns: comprise multiselect one, sequential testing; Call the test result of LCR on the other hand, handle the impedance information database of back show electrode, the impedance-frequency curve and the impedance-voltage curve of electrode.
As shown in Figure 4, three of present embodiment kinds of courses of work are specifically:
1, the testing impedance under given stimulation voltage and the frequency condition:
The database of a, newly-built tested membrane electrode for the membrane electrode of having surveyed, is then opened the newly-built record in legacy data storehouse;
B, the stimulation voltage (50mV) and the frequency (1kHz) of regulating the LCR analyser;
C, selection test pattern: multiselect one test or sequential testing;
D, handle and show test results: be presented at the resistance value tabulation of thin film flexible electrod-array under given voltage and the frequency condition, multiselect one pattern then shows the test result of selected electrode.
2, given stimulation voltage, the testing impedance under the conversion frequency of stimulation condition and demonstration impedance-frequency relation curve:
The database of a, newly-built tested membrane electrode for the membrane electrode of having surveyed, is then opened the newly-built record in legacy data storehouse;
B, the stimulation voltage (50mV) of regulating the LCR analyser and frequency (20Hz～10kHz);
C, under a certain frequency the default sequence test pattern;
D, be presented at the electrode impedance value list under a certain frequency;
E, conversion frequency of stimulation show the electrode impedance value list under this frequency; Repeat this step until finishing test request;
F, handle the impedance of all data and show electrode and the relation curve of frequency.
3, given frequency of stimulation, testing impedance under the conversion stimulation voltage condition and demonstration impedance voltage relation curve:
The database of a, newly-built tested membrane electrode for the membrane electrode of having surveyed, is then opened the newly-built record in legacy data storehouse;
B, stimulation voltage (10mV～900mV) and the frequency (1kHz) of regulating the LCR analyser;
C, under a certain stimulation voltage the default sequence test pattern;
Electrode impedance value list under d, a certain stimulation voltage of demonstration;
E, conversion stimulation voltage show the electrode impedance value list under this voltage; Repeat this step until finishing test request;
F, handle the impedance of all data and show electrode and the relation curve of voltage.
Present embodiment has replaced the impedance test device that relies on the manual change interface channel originally, can select tested membrane electrode automatically, and testing efficiency height and test error are little; Simultaneously concrete extensibility can be applicable to the membrane electrode of arranging; Device is simple, cost is low, man-machine interface close friend, and can programme according to different test requests, make things convenient for the tester to operate.
Claims (6)
1. the automatic impedance-testing system of a flexible film microelectrode arrays, it is characterized in that, comprise: the LCR analyser, computing machine, electrode connection device and multiplexer circuit, wherein: an end of electrode connection device links to each other with multiplexer circuit and transmits control word information, the other end of the electrode connection device transmitting test data information that links to each other with electrode to be measured, the multiplexer circuit transmitting test data information that links to each other with the LCR analyser, the LCR analyser transmitting test data information that links to each other with electrode to be measured, the computing machine transmission control word information that links to each other with multiplexer circuit, the computing machine transmitting test data information that links to each other with the LCR analyser;
Described electrode connection device comprises: four dog screws, probe circuit plate and base plates, and wherein: dog screw is positioned at the endpoint location of probe circuit plate and base plate, and probe circuit plate and base plate are fixedly linked by dog screw;
Described multiplexer circuit comprises: microcontroller and signal processor, wherein: microcontroller links to each other with computing machine and transmits control word information, the input end of the signal processor transmitting test data information that links to each other with the LCR analyser, the output terminal of the signal processor transmitting test data information that links to each other with electrode connection device, the microcontroller transmitting test data information that links to each other with signal processor.
2. the automatic impedance-testing system of flexible film microelectrode arrays according to claim 1 is characterized in that, described dog screw is provided with spring.
3. the automatic impedance-testing system of flexible film microelectrode arrays according to claim 1, it is characterized in that, described probe circuit plate comprises: circuit board, probe array, observation panel and connector, wherein: circuit board is fixed on the base plate, probe array is welded on the circuit board, observation panel is arranged on a side of circuit board, and connector is arranged on the circuit board, and connector links to each other with multiplexer circuit.
4. the automatic impedance-testing system of flexible film microelectrode arrays according to claim 3, it is characterized in that, described probe array comprises the spring probe of some round ends, the diameter of this probe is less than tested membrane electrode pad, and the spread pattern of probe array is identical with the spread pattern of tested membrane electrode pad.
5. the automatic impedance-testing system of flexible film microelectrode arrays according to claim 1 is characterized in that, described base plate is provided with two anchor clamps.
6. the automatic impedance-testing system of flexible film microelectrode arrays according to claim 5 is characterized in that, described anchor clamps are packaged types.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102279378A (en) * | 2011-07-05 | 2011-12-14 | 江苏东方航天校准检测有限公司 | Input impedance measurement device of active alternating current (ac) voltage measurement circuit or apparatus and method thereof |
| CN105353222A (en) * | 2015-09-25 | 2016-02-24 | 南京剑桥新港科技发展有限公司 | Diversified integrated impedance detection device based on disposable thin film electrode |
| CN106291333A (en) * | 2016-10-10 | 2017-01-04 | 京东方科技集团股份有限公司 | A kind of circuit board detecting system |
| CN107314885A (en) * | 2017-06-27 | 2017-11-03 | 太平洋（聊城）光电科技有限公司 | A kind of array type is multiple to receive that light engine is optical coupled and measurement jig |
| CN109200468A (en) * | 2018-10-22 | 2019-01-15 | 中国科学院心理研究所 | A kind of multichannel electro photoluminescence field strength spatial distribution measuring device |
| CN109381787A (en) * | 2018-09-10 | 2019-02-26 | 清华大学 | Implantation medical equipment intersects electric pulse detection method and equipment |
| CN110568022A (en) * | 2019-10-21 | 2019-12-13 | 四川大学 | high-speed measurement system and measurement method for resistance of double-sided multi-point metal coating |
| CN112666223A (en) * | 2019-12-19 | 2021-04-16 | 深圳硅基仿生科技有限公司 | Electrode aging test device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1502300A (en) * | 2002-10-24 | 2004-06-09 | 三星电子株式会社 | Apparatus and method for measuring local skin impedance using multiple array electrodes |
| CN101576523A (en) * | 2009-06-11 | 2009-11-11 | 上海交通大学 | Method for detecting tumour cells by adopting microelectrode array impedance biosensor chip |
-
2010
- 2010-03-25 CN CN201010132952A patent/CN101799495B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1502300A (en) * | 2002-10-24 | 2004-06-09 | 三星电子株式会社 | Apparatus and method for measuring local skin impedance using multiple array electrodes |
| CN101576523A (en) * | 2009-06-11 | 2009-11-11 | 上海交通大学 | Method for detecting tumour cells by adopting microelectrode array impedance biosensor chip |
Non-Patent Citations (1)
| Title |
|---|
| 《Sensors and Actuators》 20050630 Maria Kindlundh等 "A gel-based wafer-level testing procedure for microelectrodes" 557-562 1-6 第107卷, 第2期 2 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102279378A (en) * | 2011-07-05 | 2011-12-14 | 江苏东方航天校准检测有限公司 | Input impedance measurement device of active alternating current (ac) voltage measurement circuit or apparatus and method thereof |
| CN105353222A (en) * | 2015-09-25 | 2016-02-24 | 南京剑桥新港科技发展有限公司 | Diversified integrated impedance detection device based on disposable thin film electrode |
| CN105353222B (en) * | 2015-09-25 | 2018-07-17 | 南京剑桥新港科技发展有限公司 | Diversified integrated impedance detection device based on disposable thin film electrode |
| CN106291333A (en) * | 2016-10-10 | 2017-01-04 | 京东方科技集团股份有限公司 | A kind of circuit board detecting system |
| CN106291333B (en) * | 2016-10-10 | 2019-06-14 | 京东方科技集团股份有限公司 | A kind of circuit board detecting system |
| CN107314885A (en) * | 2017-06-27 | 2017-11-03 | 太平洋（聊城）光电科技有限公司 | A kind of array type is multiple to receive that light engine is optical coupled and measurement jig |
| CN109381787A (en) * | 2018-09-10 | 2019-02-26 | 清华大学 | Implantation medical equipment intersects electric pulse detection method and equipment |
| CN109381787B (en) * | 2018-09-10 | 2023-10-31 | 清华大学 | Method and apparatus for detecting cross electric pulse of implanted medical instrument |
| CN109200468A (en) * | 2018-10-22 | 2019-01-15 | 中国科学院心理研究所 | A kind of multichannel electro photoluminescence field strength spatial distribution measuring device |
| CN109200468B (en) * | 2018-10-22 | 2023-09-05 | 中国科学院心理研究所 | Multichannel electric stimulation field intensity spatial distribution measuring device |
| CN110568022A (en) * | 2019-10-21 | 2019-12-13 | 四川大学 | high-speed measurement system and measurement method for resistance of double-sided multi-point metal coating |
| CN112666223A (en) * | 2019-12-19 | 2021-04-16 | 深圳硅基仿生科技有限公司 | Electrode aging test device |
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