CN204058481U - A kind of neural axon tractive growing apparatus - Google Patents
A kind of neural axon tractive growing apparatus Download PDFInfo
- Publication number
- CN204058481U CN204058481U CN201420466056.1U CN201420466056U CN204058481U CN 204058481 U CN204058481 U CN 204058481U CN 201420466056 U CN201420466056 U CN 201420466056U CN 204058481 U CN204058481 U CN 204058481U
- Authority
- CN
- China
- Prior art keywords
- tractive
- block
- growing apparatus
- neural axon
- seat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
Links
Abstract
The utility model discloses a kind of neural axon tractive growing apparatus, be made up of with tractive Controlling System and mechanism two portions cultivation.Wherein, cultivate and include cell culture incubator, upper computer, controller and stepper-motor with tractive Controlling System, mechanism includes the shaft coupling, ball screw straight line slide unit, tractive contiguous block, cell traction growing apparatus, device support stand, the base that connect stepper-motor.Controller connects and Driving Stepping Motor rotates, and drive the ball screw straight line slide unit of shaft coupling one end to produce displacement, cell traction growing apparatus is fixed on device support stand, by being fixed on tractive contiguous block on straight line slide unit and tractive neural axon indirectly.Different input instructions can be set, the direction of growth, the speed of regulation and control neural axon by controller.In addition, tractive film and counterdie can plating electrode contacts, be connected with multi-channel nerve signal record stimulating system, for recording the nerve signal of aixs cylinder, and selective stimulating being carried out to different loci.
Description
Technical field
The utility model belongs to biomedicine experiment apparatus field, more specifically, relates to a kind of neural axon tractive growing apparatus.
Background technology
According to the data that American National Spinal injury statistics center 2011 is announced, current American is about survived 265,000 routine patients with spinal cord injury, every year on average newly-increased about 12,000 routine patients with spinal cord injury.In China, annual newly-increased about 60,000 Patients of Spinals.Current clinical treatment Spinal injury method mainly comprises operation, pharmacological agent and long-term motion rehabilitation etc.But a large amount of loss of nervous tissue and the exhaustion of refresh function, make Current therapeutic means very limited.Present stage, mainly carry out CO2 laser weld by nerve autograft clinically, damage defect can not only be filled, and an inner can be provided to blocking neural near-end tip extension, but, autologous nerve due to originate limited, diameter is tiny etc., and reason cannot meet at all repairs and damage neural demand in a large number, and can permanently be lost function by the nerve after intercepting, and has the neuromatous risk of formation.
In recent years, the study hotspot in neurotization and CO2 laser weld field is become by the external cultivation of neurocyte or the regeneration of stem cell inducing nerve cell.But the nervous tissue of extracorporeal culture is often because the direction of growth is disorderly and unsystematic, and after transplanting, body function recovers still fewer.How to allow the neural direction growth to contributing to functional rehabilitation govern the development of CO2 laser weld technology always.Modern biotechnology research shows, mechanics factor plays vital effect to the growth of cell, differentiation and propagation.In addition, existing lot of documents shows, electricity irritation can carry out neuromodulation effectively, Acceleration of nerve regeneration and function reparation.Therefore, the neural axon tractive growing apparatus that this research relates to can implement mechanical stretch and electricity irritation to the neural axon cultivated, and promotes the orientation of neural axon, grows fast, for neurotization and function reparation provide advanced organizational project means.
Chinese utility model patent 200910009465.2 discloses a kind of traction apparatus attached cell being carried out to circulating draw.This device drives the to-and-fro movement of Tissue Culture Dish support by motor, produces dynamic property tractive, indirect tractive attached cell to the Tissue Culture Dish be fixed on support.This device is mainly used in research Tenocyte cell biological modification under cyclic loading, the tolerance range of its Controlling System and less stable, does not meet the requirement that neural axon continues tractive growth.
Chinese utility model patent 201010583804.0 discloses a kind of high-flux culture device stimulated for cell traction.This device uses the double-deck synthetic glass AND DEWATERING FOR ORIFICE STRUCTURE being embedded with pellosil, cultivates unit and applies periodic pressure simultaneously, thus realize controlling to cell traction by using single pressure source to all cells on pellosil.This device not only can meticulous adjustment film stretching amount, property performance period tractive, but also can directly observe under inverted microscope.But the method mainly relies on the deformation of silica gel material to carry out repetition tractive to cell, makes tractive limited length.And the CO2 laser weld of human body, often need the nervous tissue of several centimetres, therefore adopt silica gel material deformation to carry out tractive, cannot cultivate the neural axon of sufficient length, this patent does not relate to the placement of stimulating electrode simultaneously, cannot implement nerve electric stimulation.
Chinese utility model patent 201110392367.9 discloses a kind of stretch-electricity combined stimulation three-dimensional cell culture.This device adopts distraction force to stimulate and electricity irritation combination loading, and under three-dimensional cell culture material being placed in the combined action of stretch stimulation and electricity irritation, analogue body inner cell living environment, promotes growth and the differentiation of cell, construct the tissue with specific function.But the length of the uncontrollable neural axon growth of this device, direction, speed, can not meet the requirement of neurotization and CO2 laser weld.
Utility model content
The purpose of this utility model is to provide a kind of neural axon tractive growing apparatus, can the short period cultivate be of convenient length in a large number, the nerve tract of arrangement regulation, for CO2 laser weld, to solve the problem that autologous nerve reparation limited source and diameter are in the past difficult to mate.The utility model additionally provides the Controlling System of this device, and controllable shaft is dashed forward the speed of tractive, step-length, time, can control many independently cell traction growing apparatus work simultaneously.
For achieving the above object, this utility model is achieved through the following technical solutions, and neural axon tractive growing apparatus forms with tractive Controlling System and mechanism two portions by cultivating.Wherein, cultivate and include cell culture incubator, upper computer, controller and stepper-motor with tractive Controlling System, mechanism includes the shaft coupling, ball screw straight line slide unit, tractive contiguous block, cell traction growing apparatus, device support stand, the base that connect stepper-motor.
Cell traction growing apparatus is located on device support stand, and be fixed on base together with stepper-motor, shaft coupling, ball screw Linear slide platform, base is positioned in cell culture incubator, ensures the culture condition of 37 DEG C of temperature, 95% relative humidity and 5% carbonic acid gas.Cell culture incubator is provided with a duct communicated with the external world, and for the data line connection control device by stepper-motor, preferably, described duct is positioned on rear side of cell culture incubator, and duct and data line junction adopt silica gel sealing.
Cell traction growing apparatus comprises: cultivate seat, lid, stayed pole, support bar, back-up block, tractive block, sheet glass, tractive film and counterdie.Cultivate the integrated rectangle structure of seat, wherein, back-up block and tractive block are positioned to be cultivated in the middle part of seat, stayed pole is positioned at the end center cultivating seat, support bar is located at the both sides of cultivating seat through back-up block, counterdie is coated silica gel and is adhered to and cultivate in seat base channel, sheet glass adheres to be cultivated in seat grooves on two sides, and height is adjustable a little less than cultivation seat both sides height.
Upper computer can control the parameter of tractive by programming, comprises the displacement of cell traction, speed, time length.Controller receives the steering order of upper computer, thus Driving Stepping Motor rotates.Stepper-motor drives the ball screw straight line slide unit of shaft coupling one end to produce linear displacement, and by being fixed on the stayed pole of the tractive contiguous block tractive cell traction device on straight line slide unit, stayed pole drives the movement of tractive film, thus indirect tractive neural axon.
The utility model has the advantage of:
(1) the utility model constructs the device that a neural axon tractive is cultivated, and can control the length of axon growth, direction, speed flexibly.Can according to client need, turn out within a short period of time be of convenient length, the nervous tissue of growth rhythm, for repairing of neural injury, be with a wide range of applications.
(2) by the tractive Controlling System that the utility model builds, tractive can be carried out to multiple independently cell traction device simultaneously, facilitate experiment contrast.Can also the different neurocyte of tractive or organize aixs cylinder, the nervous tissue of simultaneously cultivating the difference in functionalitys such as motion, sensation is transplanted.
(3) the tractive film that uses of the utility model and counterdie adopt voltalef film respectively, and this film bio-compatibility is good, transparent, and high temperature resistance high pressure, not easily deformation occurs, facilitates sterilizing, observation and tractive.
(4) the utility model can plate electrode contacts on counterdie and tractive film, is connected with multi-channel nerve signal record stimulating system, for recording nerve signal, and carries out electricity irritation to nerve.
Accompanying drawing explanation
Fig. 1 is a kind of neural axon tractive growth cell configuration schematic diagram in the utility model embodiment;
Fig. 2 is cell traction growing apparatus mechanical construction drawing in the utility model embodiment;
Fig. 3 be in the utility model embodiment cell traction growing apparatus without lid front view;
Fig. 4 is cell traction growing apparatus tractive schematic diagram in the utility model embodiment;
Fig. 5 is aixs cylinder tractive growth control structure iron in the utility model embodiment;
Fig. 6 is the schematic diagram for grouping experiment in the utility model embodiment;
Fig. 7 is the cell traction growth schematic diagram in conjunction with electricity irritation in the utility model embodiment;
Fig. 8 is counterdie or tractive film top electrode array schematic diagram in the utility model embodiment.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the utility model, and be not used in restriction the utility model.In addition, if below in described each embodiment of the utility model involved technical characteristic do not form conflict each other and just can mutually combine.
As shown in Figure 1, the device that a kind of neural axon tractive involved by the utility model is cultivated, is made up of with tractive Controlling System and mechanism two portions cultivation.Cultivate and include cell culture incubator 1, upper computer 2, controller 3 and stepper-motor 4 with tractive Controlling System, mechanism includes the shaft coupling 5, ball screw straight line slide unit 6, tractive contiguous block 7, cell traction growing apparatus 8, device support stand 9, the base 10 that connect stepper-motor.
Be provided with a duct communicated with the external world on rear side of cell culture incubator 1, the data line passing hole channel connection control device 3 of stepper-motor 4, duct and data line junction adopt silica gel sealing.Cell culture incubator 1 is sealed structure, can provide the carbonic acid gas needed for Growth of Cells, temperature and humidity environment.
Upper computer 2 can control the parameter of tractive by programming, comprises the displacement of cell traction, speed, time length.Controller 3 receives the steering order of upper computer 2, thus Driving Stepping Motor 4 rotates.Stepper-motor 4 drives the ball screw straight line slide unit 6 of shaft coupling 5 one end to produce displacement, and cell traction growing apparatus 8 is fixed on device support stand 9, by being fixed on tractive contiguous block 7 on straight line slide unit and tractive neural axon indirectly.
Wherein, described tractive contiguous block 7, device support stand 9, base 10 adopt polytetrafluoroethylmaterial material.
As shown in figs. 2 to 4, the cell traction growing apparatus involved by the utility model is rectangular structure, is made up of cultivation seat 11, stayed pole 12, lid 13, sheet glass 14, support bar 15, back-up block 16, tractive block 17, tractive film 18 and counterdie 19.
Wherein, described cultivation seat 11 is the integrated design structure.Back-up block 16 and tractive block 17 are positioned to be cultivated in the middle part of seat, can be mobile in cultivation seat 11; Stayed pole 12 is positioned at the end center cultivating seat 11; Support bar 15 is located at the both sides of cultivating seat 11, two ends fastened by screw; Counterdie 19 adheres to bottom cultivation seat 11 in sulculus, for the cultivation of cell by coating silica gel; Sheet glass 14 is adhered to by silica gel and cultivates in seat 11 grooves on two sides, and be convenient to observe, sheet glass 14 is a little less than the height cultivating seat 11 simultaneously.
Wherein, described lid 13 is C type structure, forms gap with sheet glass 14, realizes the gas circulation of internal medium.
Wherein, described back-up block 16 is T-type structure, and support bar 15 is interted in back-up block 16 both sides, back-up block 16 to be fixed on cultivate in seat 11 and to keep balance; Fluted in the middle part of back-up block 16, this groove structure is used for nested tractive block 17, and the central authorities at tractive block 17 top, by successively through in back-up block 16 and tractive block 17, are fixed in one end of stayed pole 12 by stayed pole 12 with screw.
Wherein, described tractive block 17 is wedge structure, and tractive block 17 top is stereo structure, and bottom is arcuation, coordinates the neural axon of back-up block 16 to growth to carry out tractive.Tractive block 17 center upper portion is provided with through hole, coordinates stayed pole 12 to pass; Tractive block 17 crown center is provided with screw through-hole, coordinates fixing stayed pole 12; The arc of tractive block 17 bottom is used for the traction of tractive film 18, makes tractive film extending part be affixed on counterdie 19.
Wherein, one end of described stayed pole 12 is successively through back-up block 16 and tractive block 17, and the other end then extends to be cultivated outside seat 11, and is fixed by stayed pole 12 at the centre screw cultivating seat 11 end, prevents stayed pole 12 from loosening during cell cultures.
Wherein, described tractive film 18 is rectangular clarity films, vertically sticks to the centre of back-up block 16 and tractive block 17 with silica gel.As shown in Figure 5, tractive film 18 extension and counterdie 19 are close to, and can produce mechanical stimulus by mechanical stretch to the aixs cylinder being attached at growth on tractive film 18 and counterdie 19.
Wherein, the cultivation seat 11 of described cell traction growing apparatus 8, back-up block 16, tractive block 17 all adopt polytetrafluoroethylmaterial material, and described lid 13 is pmma material, and described stayed pole 12, support bar 15 adopt stainless material.
In the present embodiment, for carrying out tractive growth to neural axon, devise a set of neural axon tractive growing apparatus.Before using, first tractive film 18 and counterdie 19 are cut to required shape, tractive film 18 side is polished into the level and smooth gradient, then by all parts washed with de-ionized water 2-3 time, then uses alcohol and ultraviolet lamp sterilization.During assembling, fastening with key screw stayed pole 12, by back-up block 16 and tractive block 17 separately, coat silica gel respectively at back-up block 16 and the corresponding side of tractive block 17, insert between back-up block 16 and tractive block 17 with tweezers gripping tractive film 18, and tractive film 18 clamps by movable support block 16.Then, cultivation seat 11 base channel inner edge coats silica gel, slowly attaches up from the one end of cultivating seat 11 kerve with tweezers gripping counterdie 19, and use one of sterilizing swab stick to press gently, unnecessary silica gel is squeezed out.After device assembling, under ultraviolet lamp, place a few hours solidifies to silica gel, then adds sterilized water and soaks a couple of days, complete to the release of silica gel acetic acid, can carry out cell cultures.Neurocyte or tissue are positioned over tractive film 18 and counterdie 19 both sides, within 100 microns, treat that the neurocyte of both sides forms Synaptic junction, mechanical stretch can be carried out to aixs cylinder.For preventing mechanical stretch displacement excessive thus making axonotmesis, the step pitch of each tractive need be designed to 1-2 μm.
In the present embodiment, as shown in Figure 5, by data line, upper computer 2 steering order is downloaded to controller 3, thus Driving Stepping Motor 4 rotates, and then drive the tractive contiguous block 7 be fixed on ball screw Linear slide platform 6 to produce displacement by shaft coupling 5, tractive contiguous block 7 drives tractive film 18 to move on counterdie 19 by stayed pole 12 and tractive block 17, thus produces tractive to the neural axon of growth on tractive film 18 and counterdie 19.
In the present embodiment, tractive film 18 and counterdie 19 adopt voltalef film, and this film bio-compatibility is good, transparent, and high temperature resistance high pressure, not easily there is deformation, facilitate sterilizing, observation and tractive.Preferably, described tractive film 18 and counterdie 19 adopt the voltalef film of 50 μm and 198 μm respectively.
Further, device support stand 9 can place multiple cell traction growing apparatus 8, can select as requested to carry out tractive to one or more cell traction growing apparatus 8.In the present embodiment, as shown in Figure 6, tractive can be carried out to multiple independently cell traction device 8, the neurocyte that tractive is different or organize aixs cylinder simultaneously, cultivate the nervous tissue of the difference in functionalitys such as motion, sensation simultaneously, carry out control experiment.
In the present embodiment, as shown in Fig. 7 ~ 8, tractive film 18 and counterdie 19 can plate electrode contacts, be connected with multi-channel nerve signal record stimulating system, for recording neural signal, and selective stimulating is carried out to neural different loci, devise the electrical stimulation scheme of a set of neural axon, counterdie 18 and tractive film 19 are a kind of flexible PCB of transparence microelectrode array.After the growth of neural axon tractive, electricity irritation can be carried out to the aixs cylinder of tractive growth, and record corresponding Electrophysiology signal.
Those skilled in the art will readily understand; the foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., all should be included within protection domain of the present utility model.
Claims (10)
1. a neural axon tractive growing apparatus, is characterized in that, comprises and cultivating and tractive Controlling System and mechanism two portions, wherein:
Cultivate and include cell culture incubator, upper computer, controller and stepper-motor with tractive Controlling System, mechanism comprises the shaft coupling, ball screw straight line slide unit, tractive contiguous block, cell traction growing apparatus, device support stand and the base that connect stepper-motor;
Cell traction growing apparatus is located on device support stand, and be fixed on base together with stepper-motor, shaft coupling, ball screw Linear slide platform, base is positioned in cell culture incubator; A duct communicated with the external world is provided with, for making stepper-motor by data connecting line connection control device on rear side of cell culture incubator;
Cell traction growing apparatus comprises: cultivate seat, lid, stayed pole, support bar, back-up block, tractive block, sheet glass, tractive film and counterdie; Cultivate the integrated rectangle structure of seat, wherein, back-up block and tractive block are positioned to be cultivated in the middle part of seat, stayed pole is positioned at the end center cultivating seat, support bar is located at the both sides of cultivating seat through back-up block, counterdie is coated silica gel and is adhered in cultivation seat base channel, and sheet glass is adhered to by silica gel to be cultivated in seat grooves on two sides, and height is a little less than cultivation seat both sides height;
Upper computer is by the parameter of programming Control tractive, and described tractive parameter comprises the displacement of cell traction, speed, time length, and controller receives the steering order of upper computer, rotates according to the tractive driving parameter stepper-motor in steering order; Stepper-motor drives the ball screw straight line slide unit of shaft coupling one end to produce linear displacement, and by being fixed on the stayed pole in the tractive contiguous block tractive cell traction device on straight line slide unit, stayed pole drives the movement of tractive film, thus indirect tractive neural axon.
2. neural axon tractive growing apparatus as claimed in claim 1, is characterized in that, the cultivation seat of described tractive contiguous block, device support stand, base and cell traction growing apparatus, back-up block and tractive block adopt polytetrafluoroethylmaterial material.
3. neural axon tractive growing apparatus as claimed in claim 1 or 2, it is characterized in that, described lid is C type structure, forms gap with sheet glass, realizes the gas circulation of internal medium.
4. neural axon tractive growing apparatus as claimed in claim 1 or 2, it is characterized in that, described back-up block is T-type structure, and support bar is interted in back-up block both sides, back-up block to be fixed on cultivate in seat and to keep balance; Fluted in the middle part of back-up block, this groove structure is used for nested tractive block, and stayed pole will successively pass in back-up block and tractive block, and one end of stayed pole is screwed the central authorities in tractive block top.
5. neural axon tractive growing apparatus as claimed in claim 1 or 2, it is characterized in that, described tractive block is wedge structure, and tractive block top is stereo structure, and bottom is arcuation, coordinates the neural axon of back-up block to growth to carry out tractive; Tractive block center upper portion is provided with through hole, coordinates stayed pole to pass; Tractive block crown center is provided with screw through-hole, coordinates fixing stayed pole; The arc of tractive block bottom is used for the traction of tractive film, makes tractive film extending part be affixed on counterdie.
6. neural axon tractive growing apparatus as claimed in claim 1 or 2, it is characterized in that, one end of described stayed pole is successively through back-up block and tractive block, the other end then extends to be cultivated outside seat, and at the centre screw cultivating seat end, stayed pole is fixed, prevent stayed pole from loosening during cell cultures.
7. neural axon tractive growing apparatus as claimed in claim 1 or 2, it is characterized in that, described tractive film is rectangular clarity films, vertically sticks to the centre of back-up block and tractive block with silica gel; Tractive film extension and counterdie are close to, and can produce mechanical stimulus by mechanical stretch to being attached at the aixs cylinder that tractive film and counterdie grow.
8. the neural axon tractive growing apparatus described in claim 1 or 2, is characterized in that, described lid is pmma material, and described stayed pole, support bar adopt stainless material.
9. neural axon tractive growing apparatus as claimed in claim 1 or 2, it is characterized in that, described tractive film and counterdie adopt voltalef film respectively.
10. neural axon tractive growing apparatus as claimed in claim 1 or 2, is characterized in that, described counterdie and tractive film is coated with electrode contacts, be connected with multi-channel nerve signal record stimulating system, for recording nerve signal, and carrying out electricity irritation to nerve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420466056.1U CN204058481U (en) | 2014-08-18 | 2014-08-18 | A kind of neural axon tractive growing apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420466056.1U CN204058481U (en) | 2014-08-18 | 2014-08-18 | A kind of neural axon tractive growing apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204058481U true CN204058481U (en) | 2014-12-31 |
Family
ID=52200031
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420466056.1U Withdrawn - After Issue CN204058481U (en) | 2014-08-18 | 2014-08-18 | A kind of neural axon tractive growing apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204058481U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104178422A (en) * | 2014-08-18 | 2014-12-03 | 华中科技大学 | Nerve axon stretching grower |
| CN106806039A (en) * | 2017-02-22 | 2017-06-09 | 重庆大学 | Rotary cell support planting unit |
-
2014
- 2014-08-18 CN CN201420466056.1U patent/CN204058481U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104178422A (en) * | 2014-08-18 | 2014-12-03 | 华中科技大学 | Nerve axon stretching grower |
| CN104178422B (en) * | 2014-08-18 | 2016-08-24 | 华中科技大学 | A kind of neural axon tractive grower |
| CN106806039A (en) * | 2017-02-22 | 2017-06-09 | 重庆大学 | Rotary cell support planting unit |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104178422B (en) | A kind of neural axon tractive grower | |
| Rochford et al. | When bio meets technology: biohybrid neural interfaces | |
| Okano et al. | Tissue engineered skeletal muscle: preparation of highly dense, highly oriented hybrid muscular tissues | |
| CN104726332B (en) | One kind cerebral tissue external printing culture systems and method | |
| CN111467575B (en) | Myocardial cell conductive microneedle patch integrated with induced pluripotent stem cell source and preparation method and application thereof | |
| CN100344960C (en) | Apparatus for irritating animal cell and recording its physiological signal and its production and using method | |
| Bellamkonda et al. | Materials for neural interfaces | |
| CN102433258A (en) | Stretch-electricity combined stimulation three-dimensional cell culture device | |
| CN102559492B (en) | Cell culture device capable of loading shear force stimulation and electrical stimulation jointly in parallel direction | |
| CN204058481U (en) | A kind of neural axon tractive growing apparatus | |
| CN109988746A (en) | A kind of mescenchymal stem cell adipogenic induction differentiation method | |
| Chung et al. | Chronic implantation of multiple flexible polymer electrode arrays | |
| CN110343614A (en) | A kind of in vitro culture device of stimulating cellular growth | |
| CN104046564B (en) | A kind of physiology environmental mechanics stimulates formula bioreactor system | |
| CN102533547A (en) | Cell culture device with shear force-uniform electric field stimulation | |
| CN203494006U (en) | Stem cell negative pressure syringe | |
| CN109628305A (en) | The multichannel differential pulling device for answering mechanics response mechanism to study for external aixs cylinder | |
| CN210012859U (en) | Multi-channel differential traction device for research on in-vitro axon stress mechanical response mechanism | |
| CN106047802A (en) | Material for promoting mesenchymal stem cell osteoblastic differentiation and preparation method and application thereof | |
| CN105727365A (en) | Heart band-aid and preparation method thereof | |
| Owens et al. | Bioprinting of nerve | |
| CN112322492B (en) | Three-dimensional traction cultivation system for nerve axons | |
| CN101966090A (en) | Tissue engineered artificial optic nerve conduit and preparation method thereof | |
| Xu et al. | Development of a miniaturized bioreactor for neural culture and axon stretch growth | |
| DE19808055A1 (en) | Tissue engineering procedure extracting and culturing cells with mechanical stimulation to simulate in-vivo growth |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20141231 Effective date of abandoning: 20160824 |
|
| C25 | Abandonment of patent right or utility model to avoid double patenting |