CN103555561B - Biomechanical generator - Google Patents
Biomechanical generator Download PDFInfo
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- CN103555561B CN103555561B CN201310524719.0A CN201310524719A CN103555561B CN 103555561 B CN103555561 B CN 103555561B CN 201310524719 A CN201310524719 A CN 201310524719A CN 103555561 B CN103555561 B CN 103555561B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a biochemical generator, which comprises a base, a dovetail guide sliding rail, a workbench main body and a biomechanical generation mechanism, wherein the dovetail guide sliding rail is perpendicular to the upper end face of the base; the workbench main body is matched with the dovetail guide sliding rail through a dovetail groove; the biomechanical generation mechanism is arranged on the upper end face of a workbench; a threaded rod is perpendicularly arranged on the upper end face of the base; a gear mechanism for driving the threaded rod to rotate is arranged in the base; a worm gear is arranged on the lower end face of the workbench main body, and is assembled with the workbench main body in a rotating way; a worm for driving the worm gear to rotate is arranged on the workbench main body; the worm gear is sleeved on the threaded rod, and is in threaded fit with the threaded rod. According to the biochemical generator, the workbench is quickly lifted through a rough adjustment device (the gear mechanism), and is lifted by a minor distance through a fine adjustment device (a worm gear and worm matching mechanism), and a crank-link mechanism is driven by a motor to drive a simulated mechanical contact of the biochemical generation mechanism to reciprocally rotate, so that virtual shear force is generated on the surface of a biological membrane on the contact by the swinging of the biological membrane.
Description
Technical field
The present invention relates to a kind of Biomechanical generator.
Background technology
Along with the development of medical science, especially the development of osteology, mixing together between itself and biomechanics is more and more tightr, at field of orthopaedics, the regression of backbone and intervertebral disk thereof is a kind of common disease, but at present the direction of its research is mainly concentrated on to the model giving mechanical stimulation and cause backbone and intervertebral disc degeneration on living animal, then cytology is passed through, molecular biology, the mechanism of regression is studied in immunology detection and analysis, such research is static research in the cytology stage, it is the analysis cell of stressed whole last current state being carried out to form and cytoskeleton, do not have device can simulate dynamic stress and the compound stress of normal human's backbone and intervertebral disk completely at present, and then cause can not the general form that occurs under continuous mechanical stimulation effect of observation of cell in a dynamic state, microtexture, cytoskeleton, the change that biology Transport occurs, just cannot carry out observational study to terminal plate of vertebral body chondrocyte at dynamic stress and compound stress.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Biomechanical generator; dynamic mechanical stimulation is provided to normal active somatic cell; this Biomechanical generator not only can continue to give mechanical stimulation; and force direction, iterative motion can be changed within the specific limits; structural strength is good; easily manufactured, cost is lower.The more important thing is and can operate in cell culture incubator environment.
For reaching above object, be achieved through the following technical solutions:
A kind of Biomechanical generator, comprising: base, the dovetail directive slide track that vertical feet upper surface is arranged, and coordinates the worktable main body of assembling and the biomechanics generating mechanism being arranged at worktable upper surface by dovetail-indent with dovetail directive slide track;
Base upper surface is vertically installed with threaded rod, and threaded rod lower end is inserted into chassis interior; Chassis interior is provided with the gear mechanism for driving threaded rod to rotate, and gear mechanism at least comprises two-stage gear;
Worktable main body lower surface is provided with turbine, and turbine and worktable main body are assembled for rotating, and namely turbine freely can rotate relative to worktable main body circumference; Worktable main body is provided with the scroll bar for driving turbine to rotate;
Cover For A Turbine is placed on threaded rod, and with threaded rod thread fit;
Worktable main body is positioned at threaded rod central axis bearing of trend and is provided with the through hole stretched out for threaded rod;
Biomechanics generating mechanism comprises: simulation mechanics contact and toggle; Toggle comprises, the first power crank, the second crank, the 3rd wobble crank and external tooth cover;
Worktable body interior is provided with power motor, and power motor output shaft and the first power crank are connected and fixed; Second crank one end is connected by the other end of rotating shaft with the first power crank, and the 3rd wobble crank one end is connected by the other end of rotating shaft with the second crank, and the 3rd wobble crank is fixed with external tooth cover; External tooth cover is arranged at worktable main body upper surface by rotating shaft, and external tooth cover central axis and worktable body normal, namely external tooth cover can it freely rotate around central axis;
Simulation mechanics contact is 4, be vertically installed in worktable main body upper surface, and each simulation mechanics contact is arranged at worktable main body upper surface by rotating shaft, and each simulation mechanics contact center axis is vertical with worktable main body upper surface, namely each simulation mechanics contact freely can rotate around its central axis; Each simulation mechanics contact is provided with and external tooth cover meshed gears, and each gear is identical;
Scroll bar stretches out in worktable bulk end and is fixed with handwheel;
First power crank is provided with at least 4 gear adjustment holes installed for the second crank;
Gear mechanism comprises: the driving gear of power input, the power gear rotated for driving threaded rod be assemblied in driving gear and power gear and respectively with the transition gear of above-mentioned two gears meshing; Driving gear, power gear and transition gear are all assemblied in chassis interior by rotating shaft, and base upper end is provided with the regulating wheel for driving driving gear to rotate;
Regulating wheel is arranged at detent, and base upper end is provided with the locking lever mechanism coordinating with detent and locate;
Adopt the present invention of technique scheme, first the position of worktable main body relative to base is adjusted, user rotates regulating wheel, and then drive driving gear, (quantity of transition gear is not limit herein in the gear unit rotation of transition gear and power gear composition, the reduction of base overall volume is realized) by increasing transition gear, power gear drives threaded rod to rotate, because turbine and threaded rod are thread fit, and then turbine drives worktable main body, rise along dovetail directive slide track or decline, then by locking lever mechanism, (motion bar of base upper end is fixed on by hinge or latch structure realizes in locking lever mechanism herein, depart from as selected the groove of motion bar then on regulating wheel rotation process motion bar and regulating wheel, when needs location regulating wheel, only need motion bar to be inserted in the groove in regulating wheel and can meet location needs, when employing latch, then need processing to be used for latch in susceptor surface and insert the hole of locating, as long as latch directly can meet location through pilot hole corresponding on the groove in regulating wheel or through hole inserted base when regulating wheel needs to locate.) location regulating wheel wheel position, rough location is determined, configuration requirement is herein: regulating wheel turns around, and threaded rod turns 5.536 circles, worktable rising 8.304mm;
Then drive scroll bar to rotate by rotating handwheel, and then drive turbine rotation, located due to threaded rod now and do not rotated, turbine rotates separately and can complete the adjustment of further position, configuration requirement is herein: can make worktable rising 0.01829mm, and microspur rises, and guarantees high precision;
After worktable body position is determined, start power motor, power motor drives the first power crank full circle revolution, drive the second crank, drive the 3rd wobble crank, because the 3rd wobble crank and external tooth cover are fixed, and external tooth cover is fixed by rotating shaft and worktable main body upper surface, and then the 3rd wobble crank to do relative to external tooth cover rotating shaft and power motor power output shaft line be swinging back and forth of symmetric line, and then external tooth cover completes the rotation of reciprocal certain angle, and then rotated by gear driven simulation mechanics contact, and then complete the virtual of biomechanics, configuration requirement is herein: the 3rd wobble crank two place maximum position angle scope, be arranged on which assembling adjustment hole of the first power crank by the second crank and determine, as preferred 4 gears, completing the 3rd wobble crank two place maximum position angle scope is respectively 3.75 °, 7.5 °, 11.25 ° and 15 °, to adapt to human body frequency low-amplitude backbone rotating range.
To sum up:
As preferably, described worktable is made by stainless steel, to adapt to incubator humidity, gas concentration lwevel.
As preferably, described simulation mechanics contact, by with culture dish biofilm contact, circulation rotating, thus twisting microbial film, make the cell of adherent growth in liquid environment, suffer liquid and biomembranous double direction shear power.
When using of the present invention, the passage cell of health need be incubated at bottom in biomembranous culture dish, culture dish is placed on worktable, worktable is made to increase fast by rack and rinion adjustment (gear mechanism), then by micromatic setting (turbine and scroll bar matching mechanism) make simulation mechanics contact and biofilm contact tight, to ensure that the microbial film that rotarily drives of simulating mechanics contact twists to produce shearing force on its surface, the power-equipment of this contrive equipment adopts driven by motor, this motor can work 500 hours, can ensure that experiment is carried out smoothly.
The invention has the advantages that the rotational shear that simulation human intervertebral disc bears when normal activity; the change of dynamic observation intervertebral disk endplate chondrocytes form, microtexture and transfer ability under particular organisms mechanical environment, the effect that research particular organisms mechanical state plays in intervertebral disc degeneration process and mechanism of action.
Above-mentioned explanation is only the general introduction of technical solution of the present invention, in order to technique means of the present invention can be better understood, and can be implemented according to the content of specification sheets, and can become apparent to allow above and other object of the present invention, feature and advantage, below especially exemplified by preferred embodiment, and coordinate accompanying drawing, be described in detail as follows.
Accompanying drawing explanation
The present invention is totally 4 width accompanying drawings, wherein:
Fig. 1 is side-looking structural representation of the present invention.
Fig. 2 is plan structure schematic diagram of the present invention.
Fig. 3 is the perspective structure schematic diagram of worktable main body of the present invention.
Fig. 4 is the cross-sectional view of base of the present invention.
In figure: 1, base, 2, dovetail directive slide track, 3, worktable main body, 4, biomechanics generating mechanism, 5, threaded rod, 6, gear mechanism, 7, turbine, 8, scroll bar, 9, mechanics contact is simulated, 10, toggle, the 11, first power crank, the 12, second crank, 13, the 3rd wobble crank, 14, external tooth cover, 15, handwheel, 16, adjustment hole is assembled, 17, driving gear, 18, power gear, 19, transition gear, 20, regulating wheel, 21, locking lever mechanism.
Embodiment
A kind of Biomechanical generator as shown in Figure 1, Figure 2, Figure 3 and Figure 4, comprise: base 1, the dovetail directive slide track 2 that vertical feet 1 upper surface is arranged, coordinates the worktable main body 3 of assembling and the biomechanics generating mechanism 4 being arranged at worktable upper surface by dovetail-indent with dovetail directive slide track 2;
Base 1 upper surface is vertically installed with threaded rod 5, and threaded rod 5 lower end is inserted into base 1 inside; Base 1 inside is provided with the gear mechanism 6 for driving threaded rod 5 to rotate, and gear mechanism 6 at least comprises two-stage gear;
Worktable main body 3 lower surface is provided with turbine 7, and turbine 7 and worktable main body 3 are assembled for rotating, and namely turbine 7 freely can rotate relative to worktable main body 3 circumference; Worktable main body 3 is provided with the scroll bar 8 for driving turbine 7 to rotate;
Turbine 7 is placed on threaded rod 5, and with threaded rod 5 thread fit;
Worktable main body 3 is positioned at threaded rod 5 central axis bearing of trend and is provided with the through hole stretched out for threaded rod 5;
Biomechanics generating mechanism 4 comprises: simulation mechanics contact 9 and toggle 10; Toggle 10 comprises, the first power crank 11, second crank 12, the 3rd wobble crank 13 and external tooth cover 14;
Worktable main body 3 inside is provided with power motor, and power motor output shaft and the first power crank 11 are connected and fixed; Second crank 12 one end is connected by the other end of rotating shaft with the first power crank 11, and the 3rd wobble crank 13 one end is connected by the other end of rotating shaft with the second crank 12, and the 3rd wobble crank 13 is fixed with external tooth cover 14; External tooth cover 14 is arranged at worktable main body 3 upper surface by rotating shaft, and external tooth cover 14 central axis is vertical with worktable main body 3, and namely external tooth cover 14 can it freely rotate around central axis;
Simulation mechanics contact 9 is 4, be vertically installed in worktable main body 3 upper surface, and each simulation mechanics contact 9 is arranged at worktable main body 3 upper surface by rotating shaft, and each simulation mechanics contact 9 central axis is vertical with worktable main body 3 upper surface, namely each simulation mechanics contact 9 freely can rotate around its central axis; Each simulation mechanics contact 9 is provided with and external tooth cover 14 meshed gears, and each gear is identical;
Scroll bar 8 stretches out in worktable main body 3 end and is fixed with handwheel 15;
First power crank 11 is provided with at least 4 assembling adjustment holes 16 installed for the second crank 12;
Gear mechanism 6 comprises: the driving gear 17 of power input, the power gear 18 rotated for driving threaded rod 5 be assemblied in driving gear 17 and power gear 18 and respectively with the transition gear 19 of above-mentioned two gears meshing; It is inner that driving gear 17, power gear 18 and transition gear 19 are all assemblied in base 1 by rotating shaft, and base 1 upper end is provided with the regulating wheel 20 for driving driving gear 17 to rotate;
Regulating wheel 20 is arranged at detent, and base 1 upper end is provided with the locking lever mechanism 21 coordinating with detent and locate;
Adopt the present invention of technique scheme, first the position of worktable main body 3 relative to base 1 is adjusted, user rotates regulating wheel 20, and then drive driving gear 17, (quantity of transition gear is not limit herein in the gear unit rotation that transition gear 19 and power gear 18 form, the reduction of base overall volume is realized) by increasing transition gear, power gear 18 drives threaded rod 5 to rotate, because turbine 7 and threaded rod 5 are thread fit, and then turbine 7 drives worktable main body 3, rise along dovetail directive slide track 2 or decline, then by locking lever mechanism 21(herein locking lever mechanism be fixed on the motion bar of base upper end by hinge or latch structure realizes, depart from as selected the groove of motion bar then on regulating wheel rotation process motion bar and regulating wheel, when needs location regulating wheel, only need motion bar to be inserted in the groove in regulating wheel and can meet location needs, when employing latch, then need processing to be used for latch in susceptor surface and insert the hole of locating, as long as latch directly can meet location through pilot hole corresponding on the groove in regulating wheel or through hole inserted base when regulating wheel needs to locate, lock-out state as shown in Figure 4, released state as shown in Figure 1.) regulating wheel 20 position of taking turns, location, rough location is determined, configuration requirement is herein: regulating wheel 20 turns around, threaded rod 5 turn of 5.536 circle, worktable rising 8.304mm;
Then scroll bar 8 is driven to rotate by rotating handwheel 15, and then drive turbine 7 to rotate, locate due to threaded rod 5 now and do not rotated, turbine 7 rotates separately and can complete the adjustment of further position, configuration requirement is herein: can make worktable rising 0.01829mm, microspur rises, and guarantees high precision;
After worktable main body 3 position is determined, start power motor, power motor drives the first power crank 11 full circle revolution, the second crank 12 is driven to rotate, drive the 3rd wobble crank 13, because the 3rd wobble crank 13 and external tooth cover 14 are fixed, and external tooth cover 14 is fixed by rotating shaft and worktable main body 3 upper surface, and then the 3rd wobble crank 13 to do relative to external tooth cover 14 rotating shaft and power motor power output shaft line be swinging back and forth of symmetric line, and then external tooth cover 14 completes the rotation of reciprocal certain angle, and then rotated by gear driven simulation mechanics contact 9, and then complete the virtual of biomechanics, configuration requirement is herein: the 3rd wobble crank 13 liang place maximum position angle scope, be arranged on which assembling adjustment hole 16 of the first power crank 11 by the second crank 12 and determine, as preferred 4 gears, completing the 3rd wobble crank 13 liang place maximum position angle scope is respectively 3.75 °, 7.5 °, 11.25 ° and 15 °, to adapt to human body frequency low-amplitude backbone rotating range.
To sum up:
As preferably, described worktable is made by stainless steel, to adapt to incubator humidity, gas concentration lwevel.
As preferably, described simulation mechanics contact, by with culture dish biofilm contact, circulation rotating, thus twisting microbial film, make the cell of adherent growth in liquid environment, suffer liquid and biomembranous double direction shear power.
When using of the present invention, the passage cell of health need be incubated at bottom in biomembranous culture dish, culture dish is placed on worktable, worktable is made to increase fast by rack and rinion adjustment, then simulation mechanics contact and biofilm contact is made closely by micromatic setting, to ensure that the microbial film that rotarily drives of simulating mechanics contact twists to produce shearing force on its surface, the power-equipment employing driven by motor of this contrive equipment, this motor can work 500 hours, can ensure that experiment is carried out smoothly.
The invention has the advantages that the rotational shear that simulation human intervertebral disc bears when normal activity; the change of dynamic observation intervertebral disk endplate chondrocytes form, microtexture and transfer ability under particular organisms mechanical environment, the effect that research particular organisms mechanical state plays in intervertebral disc degeneration process and mechanism of action.
The above, it is only preferred embodiment of the present invention, not any pro forma restriction is done to the present invention, although the present invention discloses as above with preferred embodiment, but and be not used to limit the present invention, any those skilled in the art are not departing within the scope of technical solution of the present invention, when the technology contents that appeal can be utilized to disclose is made a little change or is modified to the Equivalent embodiments of equivalent variations, in every case be the content not departing from technical solution of the present invention, according to technical spirit of the present invention to any simple modification made for any of the above embodiments, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (4)
1. a Biomechanical generator, it is characterized in that: comprise, base (1), the dovetail directive slide track (2) that vertical feet (1) upper surface is arranged, coordinates the worktable main body (3) of assembling and the biomechanics generating mechanism (4) being arranged at worktable upper surface by dovetail-indent with dovetail directive slide track (2);
Described base (1) upper surface is vertically installed with threaded rod (5), and threaded rod (5) lower end is inserted into base (1) inside; Described base (1) inside is provided with the gear mechanism (6) for driving threaded rod (5) to rotate, and gear mechanism (6) at least comprises two-stage gear;
Described worktable main body (3) lower surface is provided with turbine (7), and described turbine (7) and worktable main body (3) are assembled for rotating, namely turbine (7) can relative to worktable main body (3) circumferential movement; Described worktable main body (3) is provided with the scroll bar (8) for driving turbine (7) to rotate;
Described turbine (7) is placed on threaded rod (5), and with threaded rod (5) thread fit;
Described worktable main body (3) is positioned at threaded rod (5) central axis bearing of trend and is provided with the through hole stretched out for threaded rod (5);
Described biomechanics generating mechanism (4) comprising: simulation mechanics contact (9) and toggle (10); Described toggle (10) comprises, the first power crank (11), the second crank (12), the 3rd wobble crank (13) and external tooth cover (14);
Described worktable main body (3) inside is provided with power motor, and power motor output shaft and the first power crank (11) are connected and fixed; Described second crank (12) one end is connected by the other end of rotating shaft with the first power crank (11), 3rd wobble crank (13) one end is connected by the other end of rotating shaft with the second crank (12), and the 3rd wobble crank (13) is fixed with external tooth cover (14); Described external tooth cover (14) is arranged at worktable main body (3) upper surface by rotating shaft, and external tooth cover (14) central axis is vertical with worktable main body (3), namely external tooth cover (14) can it freely rotate around central axis;
Described simulation mechanics contact (9) is 4, be vertically installed in worktable main body (3) upper surface, and each simulation mechanics contact (9) is arranged at worktable main body (3) upper surface by rotating shaft, and each simulation mechanics contact (9) central axis is vertical with worktable main body (3) upper surface, namely each simulation mechanics contact (9) freely can be rotated around its central axis; Each simulation mechanics contact (9) is provided with and external tooth cover (14) meshed gears, and each gear is identical;
Described first power crank (11) is provided with at least 4 gear adjustment holes (16) installed for the second crank (12).
2. a kind of Biomechanical generator according to claim 1, is characterized in that: described scroll bar (8) stretches out in worktable main body (3) end and is fixed with handwheel (15).
3. a kind of Biomechanical generator according to claim 1, it is characterized in that: described gear mechanism (6) comprising: the driving gear (17) of power input, the power gear (18) rotated for driving threaded rod (5) and be assemblied in driving gear (17) and power gear (18) and respectively with the transition gear (19) of above-mentioned two gears meshing; It is inner that described driving gear (17), power gear (18) and transition gear (19) are all assemblied in base (1) by rotating shaft, and described base (1) upper end is provided with the regulating wheel (20) for driving driving gear (17) to rotate.
4. a kind of Biomechanical generator according to claim 3, it is characterized in that: (20) are arranged at detent to described regulating wheel, and base (1) upper end is provided with the locking lever mechanism (21) coordinating with detent and locate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310524719.0A CN103555561B (en) | 2013-10-29 | 2013-10-29 | Biomechanical generator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310524719.0A CN103555561B (en) | 2013-10-29 | 2013-10-29 | Biomechanical generator |
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| Publication Number | Publication Date |
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| CN103555561A CN103555561A (en) | 2014-02-05 |
| CN103555561B true CN103555561B (en) | 2015-06-03 |
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| CN201310524719.0A Expired - Fee Related CN103555561B (en) | 2013-10-29 | 2013-10-29 | Biomechanical generator |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104785886B (en) * | 2015-04-29 | 2018-06-12 | 武汉一冶钢结构有限责任公司 | A kind of method of inclined hole cutting assembly and its processing inclined |
| CN106680114A (en) * | 2017-01-19 | 2017-05-17 | 苏州大学 | Soft tissue anisotropy shearing mechanical property tester |
| CN112603285A (en) * | 2020-12-23 | 2021-04-06 | 中科彭州智慧产业创新中心有限公司 | Controllable mechanics generator |
| CN112608845B (en) * | 2021-01-11 | 2023-07-25 | 兰州大学第一医院 | Stem cell mechanical pressure bionic stimulation device |
| CN115372149B (en) * | 2022-10-24 | 2023-01-31 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Circumferential loading device, method and loading system for spine |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200999244Y (en) * | 2007-01-09 | 2008-01-02 | 重庆大学 | Cell substrate uniaxial drawing apparatus |
| CN203513666U (en) * | 2013-10-29 | 2014-04-02 | 王卫明 | Biomechanics generator |
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2013
- 2013-10-29 CN CN201310524719.0A patent/CN103555561B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200999244Y (en) * | 2007-01-09 | 2008-01-02 | 重庆大学 | Cell substrate uniaxial drawing apparatus |
| CN203513666U (en) * | 2013-10-29 | 2014-04-02 | 王卫明 | Biomechanics generator |
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