CN211394497U - Multifunctional osteochondral bioreactor - Google Patents
Multifunctional osteochondral bioreactor Download PDFInfo
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- CN211394497U CN211394497U CN201921962108.3U CN201921962108U CN211394497U CN 211394497 U CN211394497 U CN 211394497U CN 201921962108 U CN201921962108 U CN 201921962108U CN 211394497 U CN211394497 U CN 211394497U
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- osteochondral
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- 238000010008 shearing Methods 0.000 claims abstract description 30
- 238000004088 simulation Methods 0.000 claims abstract description 23
- 238000005299 abrasion Methods 0.000 claims abstract description 19
- 238000012360 testing method Methods 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 238000000227 grinding Methods 0.000 claims description 25
- 210000000988 bone and bone Anatomy 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 14
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 2
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 2
- 230000009471 action Effects 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000015572 biosynthetic process Effects 0.000 abstract description 2
- 230000000638 stimulation Effects 0.000 abstract description 2
- 239000007789 gas Substances 0.000 description 12
- 239000000956 alloy Substances 0.000 description 3
- 229910045601 alloy Inorganic materials 0.000 description 3
- 210000000845 cartilage Anatomy 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
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- 238000000034 method Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000010412 perfusion Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 210000001188 articular cartilage Anatomy 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000001612 chondrocyte Anatomy 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007907 direct compression Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000010907 mechanical stirring Methods 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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Abstract
The utility model discloses a multifunctional osteochondral bioreactor, which comprises an aseptic biological environment unit, a vertical load applying unit, a shearing force applying unit and a wear simulating unit; the abrasion simulation unit is fixed in the sterile biological environment unit; the vertical load applying unit is positioned above the shearing force applying unit and is used for adding vertical pressure to the shearing force provided by the shearing force applying unit and transmitting the mixing force to the osteochondral test sample in the abrasion simulation unit through the upright column. The utility model overcomes the shearing force that current osteochondral bioreactor can't simulate the joint simultaneously and receive and the not enough of pressure environment, has combined the advantage of the bioreactor of single action, can apply multiple mechanical stimulation, promotes the formation of functional osteochondral tissue, provides good environment for the test of osteochondral support, and vertical load's pressure range is big, and the shearing load pivot angle is big, and frequency range is wide, has enlarged application scope.
Description
Technical Field
The utility model belongs to the field of biological machinery, in particular to a multifunctional osteochondral bioreactor.
Background
Current research shows that articular cartilage is subjected to cyclic fluid shear forces, continuous static pressure, or direct compressive forces during daily activities. Therefore, a well-designed and functional osteochondral reactor should provide an environment for applying the above mechanical stimuli to the printed osteochondral scaffold. In addition, osteochondral bioreactors must also meet other requirements, such as creating a gaseous environment with suitable ratios of carbon dioxide and oxygen, precise control of pH, etc.
Currently, the common osteochondral bioreactors are: the basic principle of the mechanical stirring bioreactor is that the continuous rotation of an impeller or a paddle stirrer and other components is used for increasing the material transmission, so that the uniform distribution of nutrition and oxygen is ensured; a direct perfusion bioreactor, which is a device for extruding culture solution into a bracket to ensure that cells secrete a large amount of extracellular matrix by sensing the shear force of fluid; the rotating wall bioreactor is an improved direct perfusion bioreactor.
Although the osteochondral bioreactors can simulate the mechanical stimulation environment of the osteochondral scaffold in a human body to a certain extent, the osteochondral bioreactors can only simulate the shearing force environment of the joint and cannot simulate the action environment of static pressure and direct compression force. However, many studies have shown that both play an important role in the development and maturation of chondrocytes.
Disclosure of Invention
In order to overcome the defects that the prior osteochondral bioreactor can not simulate the shearing force and the pressure environment of the joint simultaneously, the utility model provides a multifunctional osteochondral bioreactor. The utility model discloses can combine the advantage of the bioreactor of single action, can apply multiple mechanics amazing, promote the formation of functional bone cartilage tissue, provide good environment for the test of bone cartilage support.
The utility model provides a technical scheme that its technical problem adopted is:
the multifunctional osteochondral bioreactor is characterized by comprising a sterile biological environment unit, a vertical load applying unit, a shearing force applying unit and a wear simulating unit; the abrasion simulation unit is fixed in the sterile biological environment unit; the vertical load applying unit is positioned above the shearing force applying unit and is used for adding vertical pressure into the shearing force provided by the shearing force applying unit and transmitting the mixing force to the osteochondral sample in the abrasion simulating unit through the upright column;
the sterile biological environment unit comprises a sterile biological incubator, a weight base placed on the bottom plate of the sterile biological incubator, and an upper bracket fixed right above the sterile biological incubator through a support connecting rod; the weight base is used for fixing the abrasion simulation unit; the upper bracket is used for fixing the vertical load applying unit;
the abrasion simulation unit comprises a cylinder with a cover, a flow guide disc, a sample support, a bone grinding disc and a grinding disc frame for fixing the bone grinding disc, wherein the cylinder with the cover is fixed on the weight base;
the vertical load applying unit comprises a hydraulic cylinder/air cylinder fixed below the upper bracket and a hydraulic pump/air pump for driving the hydraulic cylinder/air cylinder; a pressure plate fixedly connected is arranged at the end part of a piston rod of the hydraulic cylinder/air cylinder;
the shearing force applying unit comprises a driving motor, a disc with an eccentric upright post and a variable radial eccentric disc, wherein the disc is connected with an output shaft of the driving motor; the variable radial eccentric disc is provided with a connecting part with a groove, and an eccentric upright post on the disc is inserted into the groove; the upper surface of the variable radial eccentric disc is fixed with the thrust ball bearing, the lower bottom surface of the variable radial eccentric disc is connected with one end of the upright post through a bolt, and the other end of the upright post penetrates through a through hole formed in the top surface of the sterile biological incubator to be connected with a grinding disc rack in the abrasion simulation unit through a bolt.
The utility model has the advantages that:
(1) the problem of difficulty in biological culture and test of the osteochondral is solved, and hardware support is provided for further research. Particularly, the mixing of the shear load and the vertical load is solved, so that the osteochondral bioreactor is more practical, and a better foundation is laid for laboratory test and biological osteochondral culture;
(2) the utility model discloses improved accuracy problem, controllability problem, shearing load's angle and frequency control, gas-liquid water conservancy diversion circulation problem to vertical load respectively, vertical load's pressure range is big, shearing load pivot angle is big, frequency range is wide, wherein vertical load's pressure range 0 ~ 1t, shearing load pivot angle-30- +30, frequency range 0.15Hz ~ 10Hz, can simulate different weight, asynchronous attitude, the quantitative experiment of different step speeds, enlarged application scope;
(3) the thrust ball bearing in the utility model is designed to ensure that the vertical load and the shearing load are not influenced mutually, only the load is transmitted, and the stability of the air pump device is not influenced;
(4) the sample support in the utility model is honeycombed, can provide a gas-liquid channel while containing osteochondral samples, can simultaneously carry out the same environmental test on a plurality of samples, and ensures that the culture solution circulates smoothly;
(5) the utility model provides a flow guide disc design can make the culture solution mix more evenly to the unknown influence of rotatory vortex to the osteochondral tissue has been reduced.
Drawings
FIG. 1 is a functional perspective view of the multi-functional osteochondral bioreactor of the present invention;
FIG. 2 is a schematic diagram of the two-dimensional structure of the multi-functional osteochondral bioreactor of the present invention;
FIG. 3 is an enlarged partial schematic view of the wear simulation unit of FIG. 2;
FIG. 4 is a schematic view of the gas-liquid passage location in the wear simulation unit;
FIG. 5 is a schematic structural view of a shear force application unit;
the reference numbers are as follows: 1 shear force application unit, 11 drive motors, 12 disks, 1201 eccentric columns, 13 variable radial eccentric disks, 1301 slots, 14 thrust ball bearings, 2 sterile biological environment unit, 21 sterile biological incubator, 2101 liquid inlet, 2102 liquid outlet, 2103 gas inlet, 2104 gas outlet, 22 weight base, 23 support link, 24 upper bracket, 3 vertical load application unit, 31 hydraulic cylinder/air cylinder, 32 hydraulic pump/air pump, 33 pressure disk, 4 abrasion simulation unit, 41 cylinder, 42 grinding disk rack, 43 bone grinding disk, 44 sample bracket, 45 diversion disk, 5 columns.
Detailed Description
The multifunctional osteochondral bioreactor shown in fig. 1 comprises a sterile biological environment unit 2, a vertical load applying unit 3, a shearing force applying unit 1 and a wear simulating unit 5; its main function is to apply vertical and shear loads to a sample of the osteochondral implant, providing loading conditions that mimic the in vivo environment.
As shown in fig. 2, the sterile biological environment unit is composed of a sterile biological incubator 21, a weight base 22, and an upper bracket 24 which is fixed right above the sterile biological incubator 21 by a support 23 link rod; the upper bracket 24 is connected to the sterile biological environment unit 2 by a support link 23. The weight base 22 is used for fixing the abrasion simulation unit 4 and can keep stability in the process of applying acting force; the upper bracket 24 is used to fix the vertical load applying unit 3; the aseptic biological incubator 21 is used to provide a sterile environment of constant temperature and humidity.
The abrasion simulation unit 4 consists of a cylinder 41 with a cover, a flow guide disc 45, a sample bracket 44, a bone grinding disc 43 and a grinding disc frame 42, and the enlarged schematic diagram of the abrasion simulation unit is shown in fig. 3; the guide disc 45, the sample support 44, the bone grinding disc 43 and the grinding disc frame 42 are sequentially arranged in the cylinder 41 from bottom to top, and the centers of the guide disc 45, the sample support 44, the bone grinding disc 43 and the grinding disc frame 42 are all on the axis of the cylinder 41; the grinding disc holder 42 is used to fix the bone grinding disc 43.
As the preferred embodiment of the present invention, the diversion plate 45 is an inverted cone structure, and a plurality of vertical through holes are provided between the conical surface and the plane.
And a pressure sensor is arranged below the flow guide disc and is matched with the vertical load applying unit to perform dynamic vertical pressure control.
The sample support 44 is provided with a plurality of through stepped holes for mounting bone cartilage samples, the bone cartilage samples are in contact with an alloy bone grinding disc, and a grinding disc made of specific materials can be fixed on the grinding disc. As the preferred embodiment in the utility model, the sample support is made by the alloy, is equipped with six shoulder holes on the sample support, can carry out six osteochondral test with environmental test simultaneously.
A liquid inlet 2101 is arranged at the lower part of the side surface of the cylinder, and a liquid outlet 2102 is arranged at the upper part of the side surface of the cylinder and is respectively connected with an external culture solution inlet pipeline and an external culture solution outlet pipeline; the cylinder top cover is provided with a gas inlet 2103 and a gas outlet 2104 for renewing the gas environment in the wear simulation unit.
The cylinder is made of transparent materials, so that the test progress can be conveniently observed in real time in the test process, and preferably, the cylinder is made of polymethyl methacrylate.
The sample holder 44 of the wear simulation unit is fixed to the inner wall of the cylinder 41 by a plug.
The vertical load applying unit 3 is composed of an air cylinder and an air pump; a pressure plate 33 fixedly connected with the end part of a piston rod of the cylinder is arranged, and the pressure plate 33 is made of alloy and has high hardness; the vertical load applying unit 3 is coupled to the upper bracket 24 by means of bolt-and-nut coupling. The piston rod of the cylinder moves up and down under the action of the air pump, drives the pressure plate connected with the piston rod of the cylinder to move up and down, applies vertical pressure to the thrust ball bearing 14 below the pressure plate 33, transmits the vertical pressure to the shearing force applying unit 1 below the thrust ball bearing 14, and applies vertical pressure to the culture through the support column 5 fixed with the variable radial eccentric disc 13 in the shearing force applying unit, so that the simulation vertical load is realized.
The shearing force applying unit comprises a driving motor 11, a circular disc 12 with an eccentric upright 1201 connected with an output shaft of the driving motor 11, and a variable radial eccentric disc 13; the variable radial eccentric disc 13 is rotated by the driving motor 11 to swing back and forth within a certain angle range to generate a shear load with adjustable frequency and angle, wherein the swing angle is 60 degrees (-30 degrees to +30 degrees), and the swing motion drives the support 5 fixed with the variable radial eccentric disc 13 to rotate and contact with the culture in the abrasion simulation unit 4 to realize the application of the shear force; in actual operation, the vertical pressure applied by the vertical load applying unit and the shearing force applied by the shearing force applying unit are simultaneously transmitted by the support column 5, and the vertical load and the shearing load are not influenced by each other due to the fact that the vertical pressure is transmitted through the thrust ball bearing, only the load is transmitted, and the stability of the air pump device is not influenced;
as shown in fig. 5, the variable radial eccentric disc 13 is provided with a connecting part with a groove 1301, the eccentric upright 1201 on the disc 12 is inserted into the groove 1301, the disc 12 is connected with the power output shaft of the driving motor 11, the rotation of the driving motor 11 drives the disc 12 to rotate, and the eccentric upright 1201 on the disc 12 slides in the groove of the connecting part of the variable radial eccentric disc while following the rotation of the disc, so as to drive the eccentric disc to swing; the upper surface of the variable radial eccentric disc is fixed with the thrust ball bearing, the lower bottom surface of the variable radial eccentric disc is connected with one end of the upright post through a bolt, and the other end of the upright post penetrates through a through hole formed in the top surface of the sterile biological incubator to be connected with a grinding disc rack in the abrasion simulation unit through a bolt.
As the preferred embodiment of the utility model, the center of stand, variable radial eccentric disc, pressure disk, bearing is on same vertical line.
As a preferred embodiment of the present invention, a raised cylinder is provided on the upper surface of the variable radial eccentric disc, and the thrust ball bearing is fixed to the cylinder; the diameter of the cylinder is equal to the inner diameter of the thrust ball bearing, and the height of the cylinder is smaller than that of the thrust ball bearing.
As the preferred embodiment of the present invention, the eccentric distance of the eccentric column on the disk can be adjusted through different hole positions on the disk.
In the schematic position diagram of the gas-liquid channel in the wear simulation unit shown in fig. 4, the culture solution is intermittently pressed into the aseptic biological incubator 21 through the peristaltic pump, enters from the liquid inlet 2101, is uniformly mixed and stirred through the bottom layer, and then passes through the flow guide disc to form a small vortex to enter the stepped hole on the sample support 44 to be fully contacted with the osteochondral sample. The culture solution flows through the flow guide channels reserved in the bone grinding disc 43 and the grinding disc frame 42 after overflowing the stepped hole, and reaches the upper layer liquid outlet 2102 to be discharged. The gas is communicated with the outer box of the sterile room, the gas is fed from the gas inlet 2103 through the small-sized gas pump, and the gas is discharged from the gas outlet 2104, so that the gas in the bioreactor is updated. Meanwhile, the aseptic biological incubator is constant in temperature and humidity, and the physiological growth environment of biological osteochondral is simulated.
Meanwhile, the bone millstone in the abrasion simulation unit is in contact with the osteochondral sample contained in the sample bracket under the driving of shearing motion and vertical load, thereby providing a simulated rehabilitation environment for human joint motion.
Claims (9)
1. The multifunctional osteochondral bioreactor is characterized by comprising a sterile biological environment unit, a vertical load applying unit, a shearing force applying unit and a wear simulating unit; the abrasion simulation unit is fixed in the sterile biological environment unit; the vertical load applying unit is positioned above the shearing force applying unit and is used for adding vertical pressure into the shearing force provided by the shearing force applying unit and transmitting the mixing force to the osteochondral sample in the abrasion simulating unit through the upright column;
the sterile biological environment unit comprises a sterile biological incubator, a weight base placed on the bottom plate of the sterile biological incubator, and an upper bracket fixed right above the sterile biological incubator through a support connecting rod;
the abrasion simulation unit comprises a cylinder with a cover, a flow guide disc, a sample support, a bone grinding disc and a grinding disc frame for fixing the bone grinding disc, wherein the cylinder with the cover is fixed on the weight base;
the vertical load applying unit comprises a hydraulic cylinder/air cylinder and a hydraulic pump/air pump which are fixed below the upper bracket; a pressure plate fixedly connected is arranged at the end part of a piston rod of the hydraulic cylinder/air cylinder;
the shearing force applying unit comprises a driving motor, a disc with an eccentric upright post and a variable radial eccentric disc, wherein the disc is connected with an output shaft of the driving motor; the variable radial eccentric disc is provided with a connecting part with a groove, and an eccentric upright post on the disc is inserted into the groove; the upper surface of the variable radial eccentric disc is fixed with a thrust ball bearing, the lower bottom surface of the variable radial eccentric disc is connected with one end of the upright post through a bolt, and the other end of the upright post passes through a through hole formed in the top surface of the sterile biological incubator to be connected with a grinding disc rack in the abrasion simulation unit through a bolt.
2. The multifunctional osteochondral bioreactor of claim 1, wherein the flow guiding plate is an inverted cone structure with a plurality of vertical through holes between the cone and the plane.
3. The multifunctional osteochondral bioreactor of claim 1, wherein a pressure sensor is disposed below the flow guide plate.
4. The multifunctional osteochondral bioreactor of claim 1, wherein the sample holder has a plurality of stepped holes therethrough; the osteochondral test sample is mounted in the stepped hole and contacts the bone grinding disc.
5. The multifunctional osteochondral bioreactor of claim 1, wherein the cylinder has a liquid inlet at a lower portion of the side surface and a liquid outlet at an upper portion, and the liquid inlet and the liquid outlet are connected to an external culture solution inlet pipe and an external culture solution outlet pipe, respectively; the cylinder upper cover is provided with a gas inlet and a gas outlet.
6. The multifunctional osteochondral bioreactor of claim 1, wherein the cylinder is made of polymethylmethacrylate.
7. The multifunctional osteochondral bioreactor of claim 1, wherein the sample holder of the abrasion simulation unit is fixed to the inner wall of the cylinder by a latch.
8. The multifunctional osteochondral bioreactor of claim 1, wherein the variable radial eccentric disc has a raised cylinder on its upper surface, and the thrust ball bearing is fixed on the cylinder.
9. The multifunctional osteochondral bioreactor of claim 8, wherein the cylinder has a diameter equal to an inner diameter of the thrust ball bearing, and a height smaller than that of the thrust ball bearing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921962108.3U CN211394497U (en) | 2019-11-14 | 2019-11-14 | Multifunctional osteochondral bioreactor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921962108.3U CN211394497U (en) | 2019-11-14 | 2019-11-14 | Multifunctional osteochondral bioreactor |
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| CN211394497U true CN211394497U (en) | 2020-09-01 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110777074A (en) * | 2019-11-14 | 2020-02-11 | 浙江大学 | Multifunctional osteochondral bioreactor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110777074A (en) * | 2019-11-14 | 2020-02-11 | 浙江大学 | Multifunctional osteochondral bioreactor |
| CN110777074B (en) * | 2019-11-14 | 2024-05-28 | 浙江大学 | Multifunctional osteochondral bioreactor |
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