CN203878154U - Three-dimensional-gyration replaceable culture fluid cell microgravity device - Google Patents
Three-dimensional-gyration replaceable culture fluid cell microgravity device Download PDFInfo
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- CN203878154U CN203878154U CN201420260812.5U CN201420260812U CN203878154U CN 203878154 U CN203878154 U CN 203878154U CN 201420260812 U CN201420260812 U CN 201420260812U CN 203878154 U CN203878154 U CN 203878154U
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Abstract
The utility model relates to a three-dimensional-gyration replaceable culture fluid cell microgravity device which comprises a cell culture fluid unit, a cell culture fluid replacement unit and a control unit, wherein the cell culture fluid unit drives a first motor to rotate around the shaft under the control of the control unit; the cell culture fluid replacement unit controls a second motor to rotate around a shaft with the same axis, frequency and phase as the first motor; when the second motor rotates around the shaft with the same axis, frequency and phase as the first motor, a cell replacement fluid outlet pipe and a cell replacement fluid inlet pipe are simultaneously aligned with a cell replacement fluid inlet port and a cell replacement fluid outlet port; and under the driving action of a hydraulic unit, the cell replacement fluid outlet pipe and cell replacement fluid inlet pipe are simultaneously inserted into the cell replacement fluid inlet port and cell replacement fluid outlet port,and the cell culture fluid replacement unit departs from the cell culture fluid unit. By utilizing the cell simulated microgravity gyration system, the three-dimensional-gyration replaceable culture fluid cell microgravity device can automatically replace the culture fluid, thereby ensuring the cells to have sufficient nutrients and be free of metabolite interference in the long-time-interval gyration experiment.
Description
Technical field
The utility model relates to a kind of biomedicine experiment equipment, particularly the replaceable nutrient solution cell microgravity device of three-dimensional revolution.
Background technology
Along with the fast development of Space Science and Technology, the step that the mankind explore to deep space strides forward day by day in recent years.Meanwhile, various biological effects and the physiology process of (particularly weightlessness/microgravity environment) Living organism in space environment, the demand of more furtheing investigate from cell levels, molecular biology horizontal development is also in continuous increase.But realistic problem is, spaceship, space shuttle and space station technical qualification are limited, and cell biological sample carries with high costs, also conventionally need spacefarer's manual operation cooperation halfway, just can obtain comparatively desirable experimental data, so it is large to implement difficulty, feasibility is not high.So, only relying in true space environment and carry out Cell Biology Experiment, the impact of research weightlessness/microgravity is unpractical, we need to find and develop can be at mode and the plant and instrument of ground simulation weightlessness/microgravity environment culturing cell.
Demand based on such, gyrator (Clinostat) is this ancient and simple laboratory apparatus is subject to people's attention again.Traditional gyrator rotates around own level axle, and is furnished with and can loads by the platform of test agent (plate-like or cylindric).In the world First gyrator by German botanist Feng saxophone in utility model (Von Sachs FGJR. Ueber Ausschliessung der geotropischen und heliotroposchen Kr ü mmungen w rend des Wachsthums. W ü rzburger Arbeiten in 1879,2:209-225,1879), be mainly used at first studying " geotropism " phenomenon of plant roots and stems.Afterwards for the demand of base simulated weightlessness/microgravity experiment contentedly, also there are a series of differentiation in the structure of gyrator, function and manufacture craft: from early stage manually, gear drive is to electronic, from single shaft to twin shaft, from 2 d-to-3 d, from constant speed to speed change, from without on-line monitoring to can microscopic examination etc., of a great variety, pattern is different.On gyrator, although biological specimen still in gravity field, is subject to constant gravitational vector effect.But due to the rotation of gyrator, the direction of motion of the biological specimen that it carries constantly changes, have little time all the time the gravity on certain orientation to make response (every kind of biology has a minimum impression time or claims time of response threshold value gravity).Gravitational vector shows as equalization, and vector is close to " zero ", i.e. so-called " zero gravity ", thereby the biological effect of the simulated weightlessness of showing/microgravity.
On three-dimensional gyrator structures, be designed with two separate pivoted arms, drive respectively inside casing and housing to rotate.In in working process, rotating speed, the turning direction of housing are all random variation, and the biological specimen that is placed on inside casing has little time to respond gravity, and the effect of similar spaces microgravity environment appears in process of growth.In the middle of practice, on three-dimensional gyrator, the process of biological specimen gravitational vector equalization is more random compared with the two-dimentional gyrator of single shaft, so think that the simulated weightlessness effect of this instrument is better.The equivalent universal gravity constant of general three-dimensional gyrator can reach 10
-5~ 10
-3g.Early 1990s, the municipal university of Osaka, Japan protects the grand professor of enjoying of honor and takes the lead in using three-dimensional gyrator simulated microgravity effect (Hoson T, Kamisaka S, Masuda Y, Yamashita M. Changes in plant growth processes under microgravity conditions simulated by a three-dimentional clinostat. Bot Mag, 105:53-70,1992).The three-dimensional gyrator of 1997 Nian Jing European Space Agency improvement, claim again " random position instrument (Random Positioning Machine; RPM) ", (Mesland DA. Novel ground-based facilities for research in the effects of weight. ESA Microgravity News formally comes into operation at Dutch spacelab, 9:5-10,1996), subsequently gradually by multinational laboratory applications in the cytobiology effect study of simulated weightlessness.In " double-shaft driving frame type gyroscope " patent application of the disclosed CN101021517 of being numbered China's in August, 2007, also recorded intimate device with RPM.
Three-dimensional gyrator is simple to operate, stable performance, and gravitational vector meaning process is more even, and can cultivate in enormous quantities adherent or suspension cell.But cell culture medium can not be changed in the revolution cabin of this gyrator in experimentation, limited its range of application in simulated weightlessness research.Under normal cultivation conditions, cell obtains the nutritive substances such as protein, amino acid, glucose, somatomedin from nutrient solution, also can constantly the meta-bolites of cell itself be discharged in nutrient solution simultaneously, and the composition of nutrient solution and pH value etc. are changed.So, in order to maintain the growth conditions that cell is good, generally need 2 ~ 3d to change fresh culture for it.And utilizing existing three-dimensional gyrator stimulated microgravity culturing cell, the revolution cabin of loading cell sample need to be airtight, to be full of a nutrient solution complete body.In turning course, in order to remain microgravity state, prevent that outside air from overflowing into, avoid bubble shearing force to affect cellularity, can not in experimentation, change nutrient solution in cabin.For a long time in this situation, will cause hydrostaticpressure in culture vessel and the nutritional status of Growth of Cells to change.Searching document is not difficult to find, the view-point of cell levels revolution simulation weightless test is only selected in 24h, 48h, 72h, the time-histories that these incubation times are relatively short conventionally.If study the impact of longer time-histories microgravity state on organism function, the restriction that can not change by nutrient solution, cell not only can not get necessary nutrient, also being easy to is affected by meta-bolites and long-time anoxic, and the interpretation that the comprehensive action of these unfavorable factors certainly will interference experiment result.But, there is not yet up to now the method that can effectively address this problem both at home and abroad and be disclosed report.
Utility model content
The purpose of this utility model is to provide a kind of under the lasting revolution of gyrator, maintenance microgravity state, can automatically change cell culture fluid, while guaranteeing long time-histories revolution simulated weightlessness, cell has enough nutrients, avoids the three-dimensional that meta-bolites disturbs and turn round replaceable nutrient solution cell microgravity device.
The purpose of this utility model realizes in the following manner, and the replaceable nutrient solution cell microgravity device of three-dimensional revolution, is characterized in that: at least comprise: integrated support frame and the control unit of cell culture fluid unit, cell culture fluid permute unit, tape spool rotation; First control unit records the starting position that cell exchange liquid relief outlet or cell exchange liquid enter mouthful, the first motor that control unit is controlled in cell culture fluid unit rotates under simulated microgravity working order with a certain frequency f, by output step-length, records the work real time position that cell exchange liquid relief outlet or cell exchange liquid enter mouthful; Control unit pivots cell culture fluid unit and cell culture fluid permute unit by controlling the overall drive motor of tape spool rotation, makes cell culture fluid permute unit form Three dimensional rotation.
Control unit is by controlled in wireless hydraulic work system, by the first oil-pressure pump, suck the oil of the first oil storage chamber to the oiling of hydro-cylinder cavity of resorption, by moving on cylinder body, make cell exchange liquid go out pipe insertion cell exchange liquid relief outlet, make cell exchange liquid enter pipe insertion cell exchange liquid and enter mouth; By cell exchange liquid oil-pressure pump, cell exchange liquid is inserted to cell culture fluid unit from cell exchange sap cavity, and longer cell culture fluid of time is put out to putting out cell exchange sap cavity, until changed longer cell culture fluid duration of service, then, cell culture fluid permute unit departs from cell culture fluid unit.
Described cell culture fluid unit comprises: the first motor, cell culture fluid housing, cell rack, cell culture fluid, cell exchange liquid relief outlet, cell exchange liquid enter mouth, the first motor shaft, the first motor is fixed in cell culture fluid housing one end, the first motor-shaft extending of the first motor is in cell culture fluid housing, the first motor shaft sleeve is connected to cell rack, cell rack is placed with cell sample, cell sample is invaded in cell culture fluid, cell culture fluid housing lower end has cell exchange liquid relief outlet and cell exchange liquid to enter mouth, cell exchange liquid enters mouth for inputting cell exchange liquid, cell exchange liquid relief outlet is for discharging longer cell culture fluid duration of service, the first motor adopts stepper-motor, during rotation by control unit by step-length record, storage cell exchange liquid relief outlet or cell exchange liquid enter the position of mouth, while working asynchronously for cell culture fluid permute unit, use.
Described cell rack has the cell frame of a plurality of placement cell samples simultaneously, and cell frame is in cell culture fluid, and during the first motor shaft work, cell frame and cell rack together rotate.
Described cell culture fluid permute unit comprises: cell exchange liquid housing, hydro-cylinder cavity of resorption, oil-pressure pump, the first oil storage chamber, the second motor shaft, the second motor, the second oil storage chamber, external slideway, hydro-cylinder epicoele, put out cell exchange sap cavity, cell exchange sap cavity, inner slide, cell exchange liquid and go out pipe, cell exchange liquid relief outlet, cell exchange liquid and enter that pipe, cell exchange liquid enter mouthful, the first motor shaft; Cell exchange liquid housing is limited in external slideway and inner slide, cell exchange liquid housing has symmetrical cell exchange sap cavity and the cell exchange sap cavity put out, cell exchange sap cavity upper end fixed cell displacement liquid oil-pressure pump, the outlet of cell exchange liquid oil-pressure pump connects cell exchange liquid and enters pipe; Putting out cell exchange sap cavity upper end has cell exchange liquid to go out pipe; Cell exchange liquid goes out pipe and cell exchange liquid, and to enter pipe symmetrical around the second motor shaft.
Described put out cell exchange sap cavity and there is a hydraulic efficiency system cell exchange sap cavity lower end, hydro-cylinder cavity of resorption in hydraulic efficiency system and hydro-cylinder epicoele communicate with the first oil storage chamber and the second oil storage chamber by the first oil-pressure pump, the second oil-pressure pump respectively, by hydro-cylinder cavity of resorption and the oiling of hydro-cylinder epicoele and emptying, make to put out cell exchange sap cavity and cell exchange sap cavity moves up and down along external slideway and inner slide, keep putting out cell exchange sap cavity and cell exchange sap cavity is steadily changed cell culture fluid.
Described hydro-cylinder cavity of resorption and hydro-cylinder epicoele, the first oil storage chamber and the second oil storage chamber, on position all near the second motor shaft.
The cell exchange liquid of described cell exchange sap cavity is the more than 2 times of cell culture fluid, so that nutrient solution is changed completely.
The integrated support frame of described tape spool rotation comprises: the 3rd motor shaft, integrated support frame, bearing, back shaft; Housing is fixedly connected with back shaft, and back shaft is fixed on integrated support frame by left and right bearing, and integrated support frame is fixing with basis, and the output shaft of the 3rd motor shaft is coaxially connected with back shaft; The 3rd motor shaft is worked under control unit is controlled, and the output shaft of the 3rd motor shaft drives back shaft to rotate, and back shaft drives housing to rotate around back shaft, and cell culture fluid unit, cell culture fluid permute unit on housing rotate around back shaft with housing; The first motor that control unit is controlled in cell culture fluid unit simultaneously rotates under simulated microgravity working order with a certain frequency f, makes cell culture fluid permute unit form Three dimensional rotation.
The utility model has the advantages that: the replaceable nutrient solution cell microgravity device of three-dimensional revolution comprises: cell culture fluid unit, cell culture fluid permute unit and control unit, cell culture fluid unit is controlled and is driven the first motor to sway by control unit, cell culture fluid permute unit is controlled the second motor by control unit and is rotated with frequency coordination around axle and the first motor coaxle, when the second motor around axle and the first motor coaxle when coordination rotates frequently, making cell exchange liquid go out pipe and cell exchange liquid enters pipe and aims at cell exchange liquid simultaneously and enter mouth and cell exchange liquid relief outlet, under hydraulic pressure unit drives, making cell exchange liquid go out pipe and cell exchange liquid enters pipe and inserts cell exchange liquid simultaneously and enter mouth and cell exchange liquid relief outlet, by cell exchange liquid oil-pressure pump, cell exchange liquid is inserted to cell culture fluid unit from cell exchange sap cavity, and by duration of service longer cell culture fluid put out to putting out cell exchange sap cavity, until change completely, subsequently, cell culture fluid permute unit departs from cell culture fluid unit.It is a kind ofly can under gyrator continues rotating state, can automatically change nutrient solution, to guarantee that cell has enough nutrients, avoids the cell simulated microgravity rotary system that meta-bolites disturbs when growing time-histories revolution simulation weightless test.
Accompanying drawing explanation
Below in conjunction with embodiment, the utility model is specifically described:
Fig. 1 is the utility model embodiment schematic diagram;
Fig. 2 is the hydraulic cylinder structure figure of Fig. 1.
In figure, 1, housing; 2, the first motor; 3, cell culture fluid housing; 4, cell rack; 5, cell culture fluid; 6, cell exchange liquid oil-pressure pump; 7, cell exchange liquid housing; 8, hydro-cylinder cavity of resorption; 9, the first oil-pressure pump; 10, the first oil storage chamber; 11, the second motor shaft; 12, the second motor; 13, the second oil storage chamber; 14, external slideway; 15, hydro-cylinder epicoele; 16, put out cell exchange sap cavity; 17, cell exchange sap cavity; 18, inner slide; 19, cell exchange liquid goes out pipe; 20, cell exchange liquid relief outlet; 21, cell exchange liquid enters pipe; 22, cell exchange liquid enters mouth; 23; The first motor shaft; 24, control unit; 25, the second oil-pressure pump; 26, the 3rd motor shaft; 27, integrated support frame; 28, bearing; 29, back shaft.
Embodiment
As shown in Figure 1, the replaceable nutrient solution cell microgravity device of three-dimensional revolution, at least comprises: integrated support frame and the control unit 24 of cell culture fluid unit, cell culture fluid permute unit, tape spool rotation; First control unit 24 records cell exchange liquid relief outlet 20 or cell exchange liquid and enters mouthfuls 22 starting position, the first motor that control unit 24 is controlled in cell culture fluid unit rotates under simulated microgravity working order with a certain frequency f, records cell exchange liquid relief outlet 20 or cell exchange liquid enter mouthfuls 22 work real time position by output step-length; Control unit 24 pivots cell culture fluid unit and cell culture fluid permute unit by controlling the overall drive motor of tape spool rotation, makes cell culture fluid permute unit form Three dimensional rotation.
While changing cell culture fluid, control unit 24 records cell exchange liquid in cell culture fluid permute unit and goes out the starting position that pipe 19 and cell exchange liquid enter pipe 21; Control unit 24 is controlled the second motor 12 in cell culture fluid permute unit and is rotated with acceleration, by speed governing, make cell exchange liquid relief outlet 20 and cell exchange liquid go out pipe 19, cell exchange liquid enter mouthfuls 22 and cell exchange liquid enter pipe 21 and carry out exactitude position, aligning accuracy depends on step-length design, make cell exchange liquid relief outlet 20 and cell exchange liquid go out pipe 19 concentric, cell exchange liquid enter mouthfuls 22 and cell exchange liquid to enter pipe 21 concentric, under simulated microgravity working order, work simultaneously;
Control unit 24 is by controlled in wireless hydraulic work system, the oil that sucks the first oil storage chamber 10 by the first oil-pressure pump 9 is to 8 oilings of hydro-cylinder cavity of resorption, by moving on cylinder body, make cell exchange liquid go out pipe 19 insertion cell exchange liquid relief outlets 20, make cell exchange liquid enter pipe 21 insertion cell exchange liquid and enter mouth 22; By cell exchange liquid oil-pressure pump 6, cell exchange liquid is inserted to cell culture fluid unit from cell exchange sap cavity 17, and by duration of service longer cell culture fluid 5 put out to putting out cell exchange sap cavity 16, until changed long cell culture fluid of time, then, cell culture fluid permute unit departs from cell culture fluid unit.
Cell culture fluid unit comprises: the first motor 2, cell culture fluid housing 3, cell rack 4, cell culture fluid 5, cell exchange liquid relief outlet 20, cell exchange liquid enter mouthful 22, first motor shaft 23, the first motor 2 is fixed in cell culture fluid housing 3 one end, the first motor shaft 23 of the first motor 2 stretches in cell culture fluid housing 3, the first motor shaft 23 is socketed with cell rack 4, cell rack 4 is placed with cell sample, cell sample is immersed in cell culture fluid 5, cell culture fluid housing 3 lower ends have cell exchange liquid relief outlet 20 and cell exchange liquid to enter mouth 22, cell exchange liquid enters mouth 22 for inputting cell exchange liquid, cell exchange liquid relief outlet 20 is for discharging longer cell culture fluid 5 duration of service, the first motor 2 adopts stepper-motor, during rotation by control unit 24 by step-length record, storage cell exchange liquid relief outlet 20 or cell exchange liquid enter the position of mouth 22, while working asynchronously for cell culture fluid permute unit, use.
Cell rack 4 has the cell frame of a plurality of placement cell samples simultaneously, and cell frame is in cell culture fluid 5, and during the first motor shaft 23 work, cell frame and cell rack 4 together rotate.
During 6 work of cell exchange liquid oil-pressure pump, produce and have pressure, pressure to make cell culture fluid produce foam.
Cell culture fluid permute unit comprises: cell exchange liquid housing 7, hydro-cylinder cavity of resorption 8, oil-pressure pump 9, the first oil storage chamber 10, the second motor shaft 11, the second motor 12, the second oil storage chamber 13, external slideway 14, hydro-cylinder epicoele 15, put out cell exchange sap cavity 16, cell exchange sap cavity 17, inner slide 18, cell exchange liquid and go out that pipe 19, cell exchange liquid relief outlet 20, cell exchange liquid enter pipe 21, cell exchange liquid enters mouthful 22, first motor shaft 23; Cell exchange liquid housing 7 is limited in external slideway 14 and inner slide 18, cell exchange liquid housing 7 has symmetrical cell exchange sap cavity 16 and the cell exchange sap cavity 17 put out, cell exchange sap cavity 17 upper end fixed cell displacement liquid oil-pressure pumps 6,6 outlets of cell exchange liquid oil-pressure pump connect cell exchange liquid and enter pipe 21; Putting out cell exchange sap cavity 16 upper ends has cell exchange liquid to go out pipe 19; Cell exchange liquid goes out pipe 19 and cell exchange liquid, and to enter pipe 21 symmetrical around the second motor shaft 11.
As shown in Figure 2, put out cell exchange sap cavity 16 and there is a hydraulic efficiency system cell exchange sap cavity 17 lower ends, hydro-cylinder cavity of resorption 8 in hydraulic efficiency system and hydro-cylinder epicoele 15 communicate with the first oil storage chamber 10 and the second oil storage chamber 13 by the first oil-pressure pump 9, the second oil-pressure pump 25 respectively, by hydro-cylinder cavity of resorption 8 and 15 oilings of hydro-cylinder epicoele and emptying, make to put out cell exchange sap cavity 16 and cell exchange sap cavity 17 and move up and down along external slideway 14 and inner slide 18, keep putting out steadily replacing cell culture fluid of cell exchange sap cavity 16 and cell exchange sap cavity 17.
In order to make cell exchange liquid housing 7 stability of rotation, hydro-cylinder cavity of resorption 8 and hydro-cylinder epicoele 15, the first oil storage chambers 10 and the second oil storage chamber 13, on position all near the second motor shaft 11.
Cell culture fluid permute unit is by the powered battery in housing 1, and cell culture fluid permute unit and control unit 24 communicate by dedicated radio link, and independent while making the work of cell culture fluid permute unit, physical condition is not subject to other structure influence.Control unit is known technology, is not just described in detail here.
The cell exchange liquid of cell exchange sap cavity 17 is the more than 2 times of cell culture fluid 5, so that nutrient solution is changed completely.
The replacing time can be passed through displacement detecting or working time control.
Hydro-cylinder is distributed in around the second motor shaft, steady while making cell culture fluid displacement.
The integrated support frame of tape spool rotation comprises: the 3rd motor shaft 26, integrated support frame 27, bearing 28, back shaft 29; Housing 1 is fixedly connected with back shaft 29, and back shaft 29 is fixed on integrated support frame 27 by left and right bearing 28, and integrated support frame 27 is fixing with basis, and the output shaft of the 3rd motor shaft 26 is coaxially connected with back shaft 29; The 3rd motor shaft 26 is worked under control unit 24 is controlled, the output shaft of the 3rd motor shaft 26 drives back shaft 29 to rotate, back shaft 29 drives housing 1 to rotate around back shaft 29, and the cell culture fluid unit on housing 1, cell culture fluid permute unit rotate around back shaft 29 with housing 1; The first motor that control unit 24 is controlled in cell culture fluid unit simultaneously rotates under simulated microgravity working order with a certain frequency f, makes cell culture fluid permute unit form Three dimensional rotation.
The parts that the present embodiment does not describe in detail and structure belong to well-known components and common structure or the conventional means of the industry, here not narration one by one.
Claims (8)
1. the replaceable nutrient solution cell microgravity device of three-dimensional revolution, is characterized in that: at least comprise: integrated support frame and the control unit (24) of cell culture fluid unit, cell culture fluid permute unit, tape spool rotation; Control unit (24) first records the starting position that cell exchange liquid relief outlet (20) or cell exchange liquid enter mouthful (22), the first motor that control unit (24) is controlled in cell culture fluid unit rotates under simulated microgravity working order with a certain frequency f, by output step-length, records the work real time position that cell exchange liquid relief outlet (20) or cell exchange liquid enter mouthful (22); Control unit (24) pivots cell culture fluid unit and cell culture fluid permute unit by controlling the overall drive motor of tape spool rotation, makes cell culture fluid permute unit form Three dimensional rotation.
2. the replaceable nutrient solution cell microgravity device of three-dimensional revolution according to claim 1, is characterized in that: described cell culture fluid unit comprises: the first motor (2), cell culture fluid housing (3), cell rack (4), cell culture fluid (5), cell exchange liquid relief outlet (20), cell exchange liquid enter mouthful (22), first motor shaft (23), the first motor (2) is fixed in cell culture fluid housing (3) one end, first motor shaft (23) of the first motor (2) stretches in cell culture fluid housing (3), the first motor shaft (23) is socketed with cell rack (4), cell rack (4) is placed with cell sample, cell sample is immersed in cell culture fluid (5), cell culture fluid housing (3) lower end has cell exchange liquid relief outlet (20) and cell exchange liquid to enter mouthful (22), cell exchange liquid enters mouthful (22) for inputting cell exchange liquid, cell exchange liquid relief outlet (20) is for discharging longer cell culture fluid duration of service (5), the first motor (2) adopts stepper-motor, during rotation by control unit (24) by step-length record, storage cell exchange liquid relief outlet (20) or cell exchange liquid enter the position of mouthful (22), while working asynchronously for cell culture fluid permute unit, use.
3. three-dimensional according to claim 2 is turned round replaceable nutrient solution cell microgravity device, it is characterized in that: described cell rack (4) has the cell frame of a plurality of placement cell samples simultaneously, cell frame is in cell culture fluid (5), during the first motor shaft (23) work, cell frame and cell rack (4) together rotate.
4. three-dimensional according to claim 1 is turned round replaceable nutrient solution cell microgravity device, it is characterized in that: described cell culture fluid permute unit comprises: cell exchange liquid housing (7), hydro-cylinder cavity of resorption (8), oil-pressure pump (9), the first oil storage chamber (10), the second motor shaft (11), the second motor (12), the second oil storage chamber (13), external slideway (14), hydro-cylinder epicoele (15), put out cell exchange sap cavity (16), cell exchange sap cavity (17), inner slide (18), cell exchange liquid goes out pipe (19), cell exchange liquid relief outlet (20), cell exchange liquid enters pipe (21), cell exchange liquid enters mouthful (22), the first motor shaft (23), cell exchange liquid housing (7) is limited in external slideway (14) and inner slide (18), cell exchange liquid housing (7) has symmetrical cell exchange sap cavity (16) and the cell exchange sap cavity (17) put out, cell exchange sap cavity (17) upper end fixed cell displacement liquid oil-pressure pump (6), cell exchange liquid oil-pressure pump (6) outlet connects cell exchange liquid and enters pipe (21), putting out cell exchange sap cavity (16) upper end has cell exchange liquid to go out pipe (19), cell exchange liquid goes out pipe (19) and cell exchange liquid, and to enter to manage (21) symmetrical around the second motor shaft (11).
5. three-dimensional according to claim 1 is turned round replaceable nutrient solution cell microgravity device, it is characterized in that: described put out cell exchange sap cavity (16) and there is a hydraulic efficiency system cell exchange sap cavity (17) lower end, hydro-cylinder cavity of resorption (8) in hydraulic efficiency system and hydro-cylinder epicoele (15) are respectively by the first oil-pressure pump (9), the second oil-pressure pump (25) communicates with the first oil storage chamber (10) and the second oil storage chamber (13), by hydro-cylinder cavity of resorption (8) and (15) oiling of hydro-cylinder epicoele and emptying, making to put out cell exchange sap cavity (16) and cell exchange sap cavity (17) moves up and down along external slideway (14) and inner slide (18), cell exchange sap cavity (16) is put out in maintenance and cell exchange sap cavity (17) is steadily changed cell culture fluid.
6. three-dimensional according to claim 1 is turned round replaceable nutrient solution cell microgravity device, it is characterized in that: described hydro-cylinder cavity of resorption (8) and hydro-cylinder epicoele (15), the first oil storage chamber (10) and the second oil storage chamber (13), position is all near the second motor shaft (11).
7. the replaceable nutrient solution cell microgravity device of three-dimensional revolution according to claim 1, is characterized in that: the cell exchange liquid of described cell exchange sap cavity (17) is the more than 2 times of cell culture fluid (5), so that nutrient solution is changed completely.
8. the replaceable nutrient solution cell microgravity device of three-dimensional revolution according to claim 1, is characterized in that: the integrated support frame of described tape spool rotation comprises: the 3rd motor shaft (26), integrated support frame (27), bearing (28), back shaft (29); Housing (1) is fixedly connected with back shaft (29), back shaft (29) is fixed on integrated support frame (27) by left and right bearing (28), integrated support frame (27) is fixing with basis, and the output shaft of the 3rd motor shaft (26) is coaxially connected with back shaft (29); The work under control unit (24) is controlled of the 3rd motor shaft (26), the output shaft of the 3rd motor shaft (26) drives back shaft (29) to rotate, back shaft (29) drives housing (1) to rotate around back shaft (29), and the cell culture fluid unit on housing (1), cell culture fluid permute unit rotate around back shaft (29) with housing (1); The first motor that control unit (24) is controlled in cell culture fluid unit simultaneously rotates under simulated microgravity working order with a certain frequency f, makes cell culture fluid permute unit form Three dimensional rotation.
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| CN201420260812.5U CN203878154U (en) | 2014-05-21 | 2014-05-21 | Three-dimensional-gyration replaceable culture fluid cell microgravity device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031835A (en) * | 2014-05-21 | 2014-09-10 | 中国人民解放军第四军医大学 | Three-dimensional rotary culture solution-replaceable cell microgravity system |
| CN111040947A (en) * | 2020-01-13 | 2020-04-21 | 中国人民解放军第四军医大学 | Liquid-changing type multicellular co-culture simulated weightlessness experimental device |
| CN111040948A (en) * | 2020-01-13 | 2020-04-21 | 中国人民解放军第四军医大学 | Liquid changing device for multi-cell co-culture simulated weightlessness experiment |
| CN115369034A (en) * | 2022-08-18 | 2022-11-22 | 潍坊医学院 | Liquid-changing type multi-cell co-culture simulated weightlessness experimental device |
-
2014
- 2014-05-21 CN CN201420260812.5U patent/CN203878154U/en not_active Withdrawn - After Issue
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031835A (en) * | 2014-05-21 | 2014-09-10 | 中国人民解放军第四军医大学 | Three-dimensional rotary culture solution-replaceable cell microgravity system |
| CN104031835B (en) * | 2014-05-21 | 2015-11-25 | 中国人民解放军第四军医大学 | The replaceable nutrient solution cell micro-gravity system of three-dimensional revolution |
| CN111040947A (en) * | 2020-01-13 | 2020-04-21 | 中国人民解放军第四军医大学 | Liquid-changing type multicellular co-culture simulated weightlessness experimental device |
| CN111040948A (en) * | 2020-01-13 | 2020-04-21 | 中国人民解放军第四军医大学 | Liquid changing device for multi-cell co-culture simulated weightlessness experiment |
| CN111040947B (en) * | 2020-01-13 | 2023-06-02 | 中国人民解放军第四军医大学 | Liquid-changing multicellular co-culture simulated weightlessness experiment device |
| CN115369034A (en) * | 2022-08-18 | 2022-11-22 | 潍坊医学院 | Liquid-changing type multi-cell co-culture simulated weightlessness experimental device |
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