CN104031835A - Three-dimensional rotary culture solution-replaceable cell microgravity system - Google Patents
Three-dimensional rotary culture solution-replaceable cell microgravity system Download PDFInfo
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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Abstract
The invention relates to a three-dimensional rotary culture solution-replaceable cell microgravity system. The system comprises a cell culture solution unit, a cell culture solution replacing unit and a control unit, wherein the cell culture solution unit is used for controlling and driving a first motor to rotate around a shaft through a control unit; the cell culture solution replacing unit is used for controlling a second motor to coaxially rotate with the first motor at the same frequency and at the same phase around the shaft through the control unit; when the second motor coaxially rotates with the first motor at the same frequency and at the same phase around the shaft, a cell replacing liquid discharging pipe and a cell replacing liquid charging pipe are simultaneously aligned to a cell replacing liquid charging hole and a cell replacing liquid discharging hole; under the driving of a hydraulic unit, the cell replacing liquid discharging pipe and the cell replacing liquid charging pipe are simultaneously inserted into the cell replacing liquid charging hole and the cell replacing liquid discharging hole; and then the cell culture solution replacing unit is separated from the cell culture solution unit. The system is a cell simulation microgravity rotary system which can be used for replacing the culture solution automatically so as to ensure that cells have enough nutrients and are free from interference of metabolic products during long-time rotation.
Description
Technical field
The present invention relates to a kind of biomedicine experiment equipment, particularly the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution.
Background technology
Along with the fast development of Space Science and Technology, the step that the mankind explore to deep space strides forward day by day in recent years.Meanwhile, various biological effects and the physiology process of (particularly weightlessness/microgravity environment) Living organism in space environment, the demand of more furtheing investigate from cell levels, molecular biology horizontal development is also in continuous increase.But realistic problem is, spaceship, space shuttle and space station technical qualification are limited, and cell biological sample carries with high costs, also conventionally need spacefarer's manual operation cooperation halfway, just can obtain comparatively desirable experimental data, so it is large to implement difficulty, feasibility is not high.So, only relying in true space environment and carry out Cell Biology Experiment, the impact of research weightlessness/microgravity is unpractical, we need to find and develop can be at mode and the plant and instrument of ground simulation weightlessness/microgravity environment culturing cell.
Based on such demand, gyrator (Clinostat) is this ancient and simple laboratory apparatus is subject to people's attention again.Traditional gyrator rotates around own level axle, and is furnished with and can loads by the platform of test agent (plate-like or cylindric).First gyrator was invented (Von Sachs FGJR. Ueber Ausschliessung der geotropischen und heliotroposchen Kr ü mmungen w rend des Wachsthums. W ü rzburger Arbeiten in 1879 by German botanist Feng saxophone in the world, 2:209-225,1879), be mainly used at first studying " geotropism " phenomenon of plant roots and stems.Afterwards for the demand of base simulated weightlessness/microgravity experiment contentedly, also there are a series of differentiation in structure, function and the manufacture craft of gyrator: from early stage manually, gear drive is to electronic, from single shaft to twin shaft, from 2 d-to-3 d, from constant speed to speed change, from without on-line monitoring to can microscopic examination etc., of a great variety, pattern is different.On gyrator, although biological specimen still in gravity field, is subject to constant gravitational vector effect.But due to the rotation of gyrator, the direction of motion of the biological specimen that it carries constantly changes, have little time all the time the gravity on certain orientation to make response (every kind of biology has a minimum impression time or claims time of response threshold value gravity).Gravitational vector shows as equalization, and vector is close to " zero ", i.e. so-called " zero gravity ", thereby the biological effect of the simulated weightlessness of showing/microgravity.
On three-dimensional gyrator structures, be designed with two separate pivoted arms, drive respectively inside casing and housing to rotate.In in working process, rotating speed, the turning direction of housing are all random variation, and the biological specimen that is placed on inside casing has little time to respond gravity, and the effect of similar spaces microgravity environment appears in process of growth.In the middle of practice, on three-dimensional gyrator, the process of biological specimen gravitational vector equalization is more random compared with the two-dimentional gyrator of single shaft, so think that the simulated weightlessness effect of this instrument is better.The equivalent universal gravity constant of general three-dimensional gyrator can reach 10
-5~ 10
-3g.Early 1990s, the municipal university of Osaka, Japan protects the grand professor of enjoying of honor and takes the lead in using three-dimensional gyrator simulated microgravity effect (Hoson T, Kamisaka S, Masuda Y, Yamashita M. Changes in plant growth processes under microgravity conditions simulated by a three-dimentional clinostat. Bot Mag, 105:53-70,1992).Three-dimensional gyrator through European Space Agency's improvement in 1997, claim again " random position instrument (Random Positioning Machine; RPM) ", (Mesland DA. Novel ground-based facilities for research in the effects of weight. ESA Microgravity News formally comes into operation at Dutch spacelab, 9:5-10,1996), subsequently gradually by multinational laboratory applications in the cytobiology effect study of simulated weightlessness.In " double-shaft driving frame type gyroscope " patent application of the disclosed CN101021517 of being numbered China's in August, 2007, also record intimate device with RPM.
Three-dimensional gyrator is simple to operate, stable performance, and gravitational vector meaning process is more even, and can cultivate in enormous quantities adherent or suspension cell.But cell culture medium can not be changed in the revolution cabin of this gyrator in experimentation, limit its range of application in simulated weightlessness research.Under normal cultivation conditions, cell obtains the nutritive substances such as protein, amino acid, glucose, somatomedin from nutrient solution, also can constantly the meta-bolites of cell itself be discharged in nutrient solution simultaneously, and composition and the pH value etc. of nutrient solution are changed.So, in order to maintain the growth conditions that cell is good, generally need 2 ~ 3d to change fresh culture for it.And utilizing existing three-dimensional gyrator stimulated microgravity culturing cell, the revolution cabin of loading cell sample need to be airtight, to be full of a nutrient solution complete body.In turning course, in order to remain microgravity state, prevent that outside air from overflowing into, avoid bubble shearing force to affect cellularity, can not in experimentation, change nutrient solution in cabin.For a long time in this situation, will cause hydrostaticpressure in culture vessel and the nutritional status of Growth of Cells to change.Searching document is not difficult to find, the view-point of cell levels revolution simulation weightless test is only selected in 24h, 48h, 72h, the time-histories that these incubation times are relatively short conventionally.If study the impact of longer time-histories microgravity state on organism function, the restriction that can not change by nutrient solution, cell not only can not get necessary nutrient, also being easy to is affected by meta-bolites and long-time anoxic, and the interpretation that the comprehensive action of these unfavorable factors certainly will interference experiment result.But, there is not yet up to now the method that can effectively address this problem both at home and abroad and be disclosed report.
Summary of the invention
The object of this invention is to provide under a kind of state that continues rotation, maintenance microgravity at gyrator, can automatically change cell culture fluid, while ensureing long time-histories revolution, cell has enough nutrients, avoids the three-dimensional that meta-bolites disturbs and turn round replaceable nutrient solution cell micro-gravity system.
Object of the present invention realizes in the following manner, and the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution, is characterized in that: at least comprise: integrated support frame and the control unit of cell culture fluid unit, cell culture fluid permute unit, tape spool rotation; First control unit records the starting position that cell exchange liquid relief outlet or cell exchange liquid enter mouthful, the first motor in control unit control cell culture fluid unit rotates under simulated microgravity working order with a certain frequency f, records by output step-length the real time position that cell exchange liquid relief outlet or cell exchange liquid enter mouthful; Control unit pivots cell culture fluid unit and cell culture fluid permute unit by the overall drive motor of controlling tape spool rotation, makes cell culture fluid permute unit form Three dimensional rotation;
Change when cell culture fluid, control unit records cell exchange liquid in cell culture fluid permute unit and goes out pipe and cell exchange liquid and enter the starting position of pipe; In control unit control cell culture fluid permute unit, the second motor rotates with acceleration, make cell exchange liquid relief outlet and cell exchange liquid go out pipe by speed governing, cell exchange liquid enters mouthful and cell exchange liquid enters pipe and carries out exactitude position, aligning accuracy depends on step-length design, make cell exchange liquid relief outlet and cell exchange liquid go out pipe with one heart, cell exchange liquid enters mouth and cell exchange liquid enters pipe with one heart, works under simulated microgravity working order simultaneously;
Control unit is by controlled in wireless hydraulic work system, suck the oil of the first oil storage chamber by the first oil-pressure pump to the oiling of hydro-cylinder cavity of resorption, make cell exchange liquid go out pipe insertion cell exchange liquid relief outlet by moving on cylinder body, make cell exchange liquid enter pipe insertion cell exchange liquid and enter mouth; By cell exchange liquid oil-pressure pump, cell exchange liquid is inserted to cell culture fluid unit from cell exchange sap cavity, and long cell culture fluid of time is put out to putting out cell exchange sap cavity, until the cell culture fluid grown of the time of having changed, then, cell culture fluid permute unit departs from cell culture fluid unit.
Described cell culture fluid unit comprises: the first motor, cell culture fluid housing, cell rack, cell culture fluid, cell exchange liquid relief outlet, cell exchange liquid enter mouth, the first motor shaft; The first motor is fixed in cell culture fluid housing one end, and the first motor-shaft extending, in cell culture fluid housing, is socketed with cell rack on axle, and cell rack is placed with cell sample, and cell sample is immersed in cell culture fluid; Cell culture fluid housing lower end has cell exchange liquid relief outlet and cell exchange liquid to enter mouth, enters mouth for inputting cell exchange liquid, the cell culture fluid that relief outlet is grown for efflux time; The first motor adopts stepper-motor, record, store cell exchange liquid relief outlet or cell exchange liquid and entered the position of mouth, use while synchronous working for cell culture fluid permute unit when rotation by control unit by step-length.
Described cell rack has the cell frame of multiple placement cell samples simultaneously, and cell frame is in cell culture fluid, and when the first motor shaft work, cell frame and cell rack together rotate.
Described cell culture fluid permute unit comprises: cell exchange liquid housing, hydro-cylinder cavity of resorption, oil-pressure pump, the first oil storage chamber, the second motor shaft, the second motor, the second oil storage chamber, external slideway, hydro-cylinder epicoele, put out cell exchange sap cavity, cell exchange sap cavity, inner slide, cell exchange liquid and go out pipe, cell exchange liquid relief outlet, cell exchange liquid and enter that pipe, cell exchange liquid enter mouthful, the first motor shaft; Cell exchange liquid housing is limited in external slideway and inner slide, cell exchange liquid housing has symmetrical cell exchange sap cavity and the cell exchange sap cavity put out, cell exchange sap cavity upper end fixed cell displacement liquid oil-pressure pump, the outlet of cell exchange liquid oil-pressure pump connects cell exchange liquid and enters pipe; Putting out cell exchange sap cavity upper end has cell exchange liquid to go out pipe; Cell exchange liquid goes out pipe and cell exchange liquid, and to enter pipe symmetrical around the second motor shaft.
Described put out cell exchange sap cavity and there is a hydraulic efficiency system cell exchange sap cavity lower end, hydro-cylinder cavity of resorption in hydraulic efficiency system and hydro-cylinder epicoele communicate with the first oil storage chamber and the second oil storage chamber by the first oil-pressure pump, the second oil-pressure pump respectively, by hydro-cylinder cavity of resorption and epicoele oiling and emptying, make to put out cell exchange sap cavity and cell exchange sap cavity moves up and down along external slideway and inner slide, guarantee to put out cell exchange sap cavity, cell exchange sap cavity is steadily changed cell culture fluid.
Described hydro-cylinder cavity of resorption and hydro-cylinder epicoele, the first oil storage chamber and the second oil storage chamber, all near the second motor shaft.
The cell exchange liquid of described cell exchange sap cavity is the more than 2 times of cell culture fluid, so that nutrient solution is changed completely.
The integrated support frame of described tape spool rotation comprises: the 3rd motor shaft, integrated support frame, bearing, back shaft; Housing is fixedly connected with back shaft, and back shaft is fixed on integrated support frame by left and right bearing, and integrated support frame is fixing with basis, and the output shaft of the 3rd motor shaft is coaxially connected with back shaft; The 3rd motor shaft is worked under control unit control, and the output shaft of the 3rd motor shaft drives back shaft to rotate, and back shaft drives housing to rotate, and cell culture fluid unit, cell culture fluid permute unit on housing rotate around back shaft with housing; The first motor in control unit control cell culture fluid unit rotates under simulated microgravity working order with a certain frequency f simultaneously, makes cell culture fluid permute unit form Three dimensional rotation.
Advantage of the present invention is: the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution comprises: cell culture fluid unit, cell culture fluid permute unit and control unit, cell culture fluid unit drives the first motor to sway by control unit control, cell culture fluid permute unit rotates with frequency coordination around axle and the first motor coaxle by control unit control the second motor, when the second motor around axle and the first motor coaxle when coordination rotates frequently, make cell exchange liquid go out pipe and cell exchange liquid enter pipe aim at simultaneously cell exchange liquid enter mouth and relief outlet, under hydraulic pressure unit drives, make cell exchange liquid go out pipe and cell exchange liquid enter pipe insert simultaneously cell exchange liquid enter mouth and relief outlet, by cell exchange liquid oil-pressure pump, cell exchange liquid is inserted to cell culture fluid unit from cell exchange sap cavity, and the cell culture fluid of growing duration of service is put out to putting out cell exchange sap cavity, until changed long cell culture fluid duration of service, then, cell culture fluid permute unit departs from cell culture fluid unit.This invention can, under the lasting state rotating of gyrator, be changed cell culture fluid automatically, and while ensureing long time-histories simulated microgravity revolution, cell has enough nutrients, avoids meta-bolites interference.
Brief description of the drawings
Below in conjunction with embodiment, the present invention is specifically described:
Fig. 1 is embodiment of the present invention schematic diagram;
Fig. 2 is the hydraulic system structure figure of Fig. 1.
In figure, 1, housing; 2, the first motor; 3, cell culture fluid housing; 4, cell rack; 5, cell culture fluid; 6, cell exchange liquid oil-pressure pump; 7, cell exchange liquid housing; 8, hydro-cylinder cavity of resorption; 9, the first oil-pressure pump; 10, the first oil storage chamber; 11, the second motor shaft; 12, the second motor; 13, the second oil storage chamber; 14, external slideway; 15, hydro-cylinder epicoele; 16, put out cell exchange sap cavity; 17, cell exchange sap cavity; 18, inner slide; 19, cell exchange liquid goes out pipe; 20, cell exchange liquid relief outlet; 21, cell exchange liquid enters pipe; 22, cell exchange liquid enters mouth; 23; The first motor shaft; 24, control unit; 25, the second oil-pressure pump; 26, the 3rd motor shaft; 27, integrated support frame; 28, bearing; 29, back shaft.
Embodiment
As shown in Figure 1, the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution, at least comprises: integrated support frame and the control unit 24 of cell culture fluid unit, cell culture fluid permute unit, tape spool rotation; First control unit 24 records cell exchange liquid relief outlet 20 or cell exchange liquid and enters mouthfuls 22 starting position, the first motor that control unit 24 is controlled in cell culture fluid unit rotates under simulated microgravity working order with a certain frequency f, records cell exchange liquid relief outlet 20 or cell exchange liquid enter mouthfuls 22 work real time position by output step-length; Control unit 24 pivots cell culture fluid unit and cell culture fluid permute unit by the overall drive motor of controlling tape spool rotation, makes cell culture fluid permute unit form Three dimensional rotation.
Change when cell culture fluid, control unit 24 records cell exchange liquid in cell culture fluid permute unit and goes out pipe 19 and cell exchange liquid and enter the starting position of pipe 21; Control unit 24 is controlled the second motor 12 in cell culture fluid permute unit and is rotated with acceleration, make cell exchange liquid relief outlet 20 and cell exchange liquid go out pipe 19 by speed governing, cell exchange liquid enter mouthfuls 22 and cell exchange liquid enter pipe 21 and carry out exactitude position, aligning accuracy depends on step-length design, make cell exchange liquid relief outlet 20 and cell exchange liquid go out pipe 19 concentric, cell exchange liquid enter mouthfuls 22 and cell exchange liquid to enter pipe 21 concentric, simultaneously under simulated microgravity working order;
Control unit 24 is by controlled in wireless hydraulic work system, suck the oil of the first oil storage chamber 10 by the first oil-pressure pump 9 to 8 oilings of hydro-cylinder cavity of resorption, make cell exchange liquid go out pipe 19 insertion cell exchange liquid relief outlets 20 by moving on cylinder body, make cell exchange liquid enter pipe 21 insertion cell exchange liquid and enter mouth 22; By cell exchange liquid oil-pressure pump 6, cell exchange liquid is inserted to cell culture fluid unit from cell exchange sap cavity 17, and the cell culture fluid 5 of growing duration of service is put out to putting out cell exchange sap cavity 16, until changed long cell culture fluid duration of service, then, cell culture fluid permute unit departs from cell culture fluid unit.
Cell culture fluid unit comprises: the first motor 2, cell culture fluid housing 3, cell rack 4, cell culture fluid 5, cell exchange liquid relief outlet 20, cell exchange liquid enter mouth 22, the first motor shaft 23, the first motor 2 is fixed in cell culture fluid housing 3 one end, the first motor shaft 23 of the first motor 2 stretches in cell culture fluid housing 3, the first motor shaft 23 is socketed with cell rack 4, cell rack 4 is placed with cell sample, cell sample is immersed in cell culture fluid 5, cell culture fluid housing 3 lower ends have cell exchange liquid relief outlet 20 and cell exchange liquid to enter mouth 22, cell exchange liquid enters mouth 22 for inputting cell exchange liquid, cell exchange liquid relief outlet 20 is for discharging long cell culture fluid 5 duration of service, the first motor 2 adopts stepper-motor, when rotation by control unit 24 by step-length record, storage cell exchange liquid relief outlet 20 or cell exchange liquid enter the position of mouth 22, while synchronous working for cell culture fluid permute unit, use.
Cell rack 4 has the cell frame of multiple placement cell samples simultaneously, and cell frame is in cell culture fluid 5, and when the first motor shaft 23 is worked, cell frame and cell rack 4 together rotate.
When cell exchange liquid oil-pressure pump 6 is worked, produce and have pressure, pressure to make cell culture fluid can not produce foam.
Cell culture fluid permute unit comprises: cell exchange liquid housing 7, hydro-cylinder cavity of resorption 8, oil-pressure pump 9, the first oil storage chamber 10, the second motor shaft 11, the second motor 12, the second oil storage chamber 13, external slideway 14, hydro-cylinder epicoele 15, put out cell exchange sap cavity 16, cell exchange sap cavity 17, inner slide 18, cell exchange liquid and go out pipe 19, cell exchange liquid relief outlet 20, cell exchange liquid and enter that pipe 21, cell exchange liquid enter mouthfuls 22, the first motor shaft 23; Cell exchange liquid housing 7 is limited in external slideway 14 and inner slide 18, what cell exchange liquid housing 7 had a position symmetry puts out cell exchange sap cavity 16 and cell exchange sap cavity 17, cell exchange sap cavity 17 upper end fixed cell displacement liquid oil-pressure pumps 6,6 outlets of cell exchange liquid oil-pressure pump connect cell exchange liquid and enter pipe 21; Putting out cell exchange sap cavity 16 upper ends has cell exchange liquid to go out pipe 19; Cell exchange liquid goes out pipe 19 and cell exchange liquid, and to enter pipe 21 symmetrical around the second motor shaft 11.
As shown in Figure 2, put out cell exchange sap cavity 16 and there is a hydraulic efficiency system cell exchange sap cavity 17 lower ends, hydro-cylinder cavity of resorption 8 in hydraulic efficiency system and hydro-cylinder epicoele 15 communicate with the first oil storage chamber 10 and the second oil storage chamber 13 by the first oil-pressure pump 9, the second oil-pressure pump 25 respectively, by hydro-cylinder cavity of resorption 8 and 15 oilings of hydro-cylinder epicoele and emptying, make to put out cell exchange sap cavity 16 and cell exchange sap cavity 17 and move up and down along external slideway 14 and inner slide 18, keep putting out steadily replacing cell culture fluid of cell exchange sap cavity 16 and cell exchange sap cavity 17.
In order to make its cell exchange liquid housing 7 stability of rotation, hydro-cylinder cavity of resorption 8 and hydro-cylinder epicoele 15, the first oil storage chambers 10 and the second oil storage chamber 13, all near the second motor shaft 11.
Cell culture fluid permute unit is by the powered battery in housing 1, and cell culture fluid permute unit and control unit 24 communicate by dedicated radio link, and independent while making the work of cell culture fluid permute unit, physical condition is not subject to other structure influence.Control unit is known technology, is not just described in detail here.
The cell exchange liquid of cell exchange sap cavity 17 is the more than 2 times of cell culture fluid 5, so that nutrient solution is changed completely.
The replacing time can be passed through displacement detecting or working time control.
Hydro-cylinder is distributed in around the second motor shaft, steady while making cell culture fluid displacement.
The integrated support frame of tape spool rotation comprises: the 3rd motor shaft 26, integrated support frame 27, bearing 28, back shaft 29; Housing 1 is fixedly connected with back shaft 29, and back shaft 29 is fixed on integrated support frame 27 by left and right bearing 28, and integrated support frame 27 is fixing with basis, and the output shaft of the 3rd motor shaft 26 is coaxially connected with back shaft 29; The 3rd motor shaft 26 is worked under control unit 24 is controlled, the output shaft of the 3rd motor shaft 26 drives back shaft 29 to rotate, back shaft 29 drives housing 1 to rotate around back shaft 29, and cell culture fluid unit, cell culture fluid permute unit on housing 1 rotate around back shaft 29 with housing 1; The first motor that control unit 24 is controlled in cell culture fluid unit simultaneously rotates under simulated microgravity working order with a certain frequency f, makes cell culture fluid permute unit form Three dimensional rotation.
The parts that the present embodiment does not describe in detail and structure belong to well-known components and common structure or the conventional means of the industry, do not do here one by one and describe.
Claims (8)
1. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution, is characterized in that: at least comprise: integrated support frame and the control unit (24) of cell culture fluid unit, cell culture fluid permute unit, tape spool rotation; Control unit (24) first records cell exchange liquid relief outlet (20) or cell exchange liquid and enters the starting position of mouthful (22), the first motor that control unit (24) is controlled in cell culture fluid unit rotates under simulated microgravity working order with a certain frequency f, is recorded cell exchange liquid relief outlet (20) or cell exchange liquid and entered the work real time position of mouth (22) by output step-length; Control unit (24) pivots cell culture fluid unit and cell culture fluid permute unit by the overall drive motor of controlling tape spool rotation, makes cell culture fluid permute unit form Three dimensional rotation;
Change when cell culture fluid, control unit (24) records cell exchange liquid in cell culture fluid permute unit and goes out pipe (19) and cell exchange liquid and enter to manage the starting position of (21); Control unit (24) is controlled the second motor (12) in cell culture fluid permute unit and is rotated with acceleration, make cell exchange liquid relief outlet (20) and cell exchange liquid go out pipe (19) by speed governing, cell exchange liquid enters mouthful (22) and cell exchange liquid and enters to manage (21) and carry out exactitude position, aligning accuracy depends on step-length design, make cell exchange liquid relief outlet (20) and cell exchange liquid go out pipe (19) concentric, cell exchange liquid enters mouthful (22) and cell exchange liquid enters pipe (21) with one heart, simultaneously under simulated microgravity working order;
Control unit (24) is by controlled in wireless hydraulic work system, the oil that sucks the first oil storage chamber (10) by the first oil-pressure pump (9) is to hydro-cylinder cavity of resorption (8) oiling, make cell exchange liquid go out pipe (19) insertion cell exchange liquid relief outlet (20) by moving on cylinder body, make cell exchange liquid enter pipe (21) insertion cell exchange liquid and enter mouthful (22); By cell exchange liquid oil-pressure pump (6), cell exchange liquid is inserted to cell culture fluid unit from cell exchange sap cavity (17), and the cell culture fluid (5) of growing duration of service is put out to putting out cell exchange sap cavity (16), until changed long cell culture fluid duration of service, then, cell culture fluid permute unit departs from cell culture fluid unit.
2. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 1, is characterized in that: described cell culture fluid unit comprises: the first motor (2), cell culture fluid housing (3), cell rack (4), cell culture fluid (5), cell exchange liquid relief outlet (20), cell exchange liquid enter mouthful (22), first motor shaft (23), the first motor (2) is fixed in cell culture fluid housing (3) one end, first motor shaft (23) of the first motor (2) stretches in cell culture fluid housing (3), the first motor shaft (23) is socketed with cell rack (4), cell rack (4) is placed with cell sample, cell sample is immersed in cell culture fluid (5), cell culture fluid housing (3) lower end has cell exchange liquid relief outlet (20) and cell exchange liquid to enter mouthful (22), cell exchange liquid enters mouthful (22) for inputting cell exchange liquid, cell exchange liquid relief outlet (20) is for discharging long cell culture fluid (5) duration of service, the first motor (2) adopts stepper-motor, record, store cell exchange liquid relief outlet (20) or cell exchange liquid and entered the position of mouth (22), use while synchronous working for cell culture fluid permute unit when rotation by control unit (24) by step-length.
3. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 2, it is characterized in that: described cell rack (4) has the cell frame of multiple placement cell samples simultaneously, cell frame is in cell culture fluid (5), when the first motor shaft (23) work, cell frame and cell rack (4) together rotate.
4. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 1, it is characterized in that: described cell culture fluid permute unit comprises: cell exchange liquid housing (7), hydro-cylinder cavity of resorption (8), oil-pressure pump (9), the first oil storage chamber (10), the second motor shaft (11), the second motor (12), the second oil storage chamber (13), external slideway (14), hydro-cylinder epicoele (15), put out cell exchange sap cavity (16), cell exchange sap cavity (17), inner slide (18), cell exchange liquid goes out pipe (19), cell exchange liquid relief outlet (20), cell exchange liquid enters pipe (21), cell exchange liquid enters mouthful (22), the first motor shaft (23), cell exchange liquid housing (7) is limited in external slideway (14) and inner slide (18), cell exchange liquid housing (7) has symmetrical cell exchange sap cavity (16) and the cell exchange sap cavity (17) put out, cell exchange sap cavity (17) upper end fixed cell displacement liquid oil-pressure pump (6), cell exchange liquid oil-pressure pump (6) outlet connects cell exchange liquid and enters pipe (21), putting out cell exchange sap cavity (16) upper end has cell exchange liquid to go out pipe (19), cell exchange liquid goes out pipe (19) and cell exchange liquid, and to enter to manage (21) symmetrical around the second motor shaft (11).
5. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 1, it is characterized in that: described put out cell exchange sap cavity (16) and there is a hydraulic efficiency system cell exchange sap cavity (17) lower end, hydro-cylinder cavity of resorption (8) in hydraulic efficiency system and hydro-cylinder epicoele (15) are respectively by the first oil-pressure pump (9), the second oil-pressure pump (25) communicates with the first oil storage chamber (10) and the second oil storage chamber (13), by hydro-cylinder cavity of resorption (8) and (15) oiling of hydro-cylinder epicoele and emptying, making to put out cell exchange sap cavity (16) and cell exchange sap cavity (17) moves up and down along external slideway (14) and inner slide (18), cell exchange sap cavity (16) is put out in maintenance and cell exchange sap cavity (17) is steadily changed cell culture fluid.
6. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 1, it is characterized in that: described hydro-cylinder cavity of resorption (8) and hydro-cylinder epicoele (15), the first oil storage chamber (10) and the second oil storage chamber (13), on position all near the second motor shaft (11).
7. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 1, is characterized in that: the cell exchange liquid of described cell exchange sap cavity (17) is the more than 2 times of cell culture fluid (5), so that nutrient solution is changed completely.
8. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 1, is characterized in that: the integrated support frame of described tape spool rotation comprises: the 3rd motor shaft (26), integrated support frame (27), bearing (28), back shaft (29); Housing (1) is fixedly connected with back shaft (29), back shaft (29) is fixed on integrated support frame (27) by left and right bearing (28), integrated support frame (27) is fixing with basis, and the output shaft of the 3rd motor shaft (26) is coaxially connected with back shaft (29); The work under control unit (24) is controlled of the 3rd motor shaft (26), the output shaft of the 3rd motor shaft (26) drives back shaft (29) to rotate, back shaft (29) drives housing (1) to rotate around back shaft (29), and cell culture fluid unit, cell culture fluid permute unit on housing (1) rotate around back shaft (29) with housing (1); The first motor that control unit (24) is controlled in cell culture fluid unit simultaneously rotates under simulated microgravity working order with a certain frequency f, makes cell culture fluid permute unit form Three dimensional rotation.
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