CN218465842U

CN218465842U - Two-axis three-dimensional rotary culture system

Info

Publication number: CN218465842U
Application number: CN202222734190.2U
Authority: CN
Inventors: 张燕
Original assignee: Suzhou Qianyun Instrument Technology Co ltd
Current assignee: Suzhou Qianyun Instrument Technology Co ltd
Priority date: 2022-10-18
Filing date: 2022-10-18
Publication date: 2023-02-10
Anticipated expiration: 2032-10-18

Abstract

The utility model discloses a three-dimensional gyration culture system of two axles, casing and cross gyration frame behind device base, equipment, device base upper end is connected with casing behind the equipment, and the casing outside is connected with the cross gyration frame behind the equipment, be connected with first fixed screw on the base plate seat. This two-axis three-dimensional gyration cultivation system has redesigned the revolving bed pedestal, and the external dimension reduces greatly, is fit for more application scenes, has increased quick location installation design to cross gyration frame inside casing simultaneously, has expanded its function. Synthesize limitation and inconvenience in the use of current product, this scheme redesign supporting special culture vessel: compared with the existing products, the system has the advantages that the application capacity of the system is greatly improved, and a better growth environment and microgravity effect simulation capacity can be provided for cell culture by matching with a special biological reaction container.

Description

Two-axis three-dimensional rotary culture system

Technical Field

The utility model relates to a cell culture technical field specifically is a two-axis three-dimensional gyration culture system.

Background

Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques.

The existing similar equipment such as a random positioning instrument, a microgravity simulator and the like are all owned by foreign companies, the product import price is high, and some models are even not exported to China due to technical blockade or are forbidden to be transported to some domestic users, so that only a small number of users can be used up and up, and the participation and research of the domestic users in the fields of cell three-dimensional culture and microgravity effect simulation culture are seriously hindered and influenced;

the existing similar products only provide a universal two-axis rotating platform, and if the platform is used in the field of cell culture, users still face a plurality of problems, such as no optional fixed frame and a matched and easy-to-use biological reaction container;

the existing similar products can not realize rapid switching between large-capacity cell culture and multi-sample cell culture application, and meanwhile, no adaptive special biological reaction container exists, which is not beneficial to the requirement of gas exchange in the long-time cell culture process;

the existing similar products cannot provide an easy-to-use solution for animal living body microgravity effect research, and researchers often have practical needs in the current biomedical research field.

Therefore, we have proposed a two-axis three-dimensional rotating cultivation system in order to solve the problems set forth above.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a two-axis three-dimensional gyration culture system to solve that above-mentioned background art provides does not have optional mount and supporting, easy to use's biological reaction container, does not have the special biological reaction container of adaptation, is unfavorable for the requirement of the long-time gas exchange who cultivates the in-process of cell, can't provide the problem that is used for the easy to use solution of animal live body microgravity effect research.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a three-dimensional gyration culture system of two axles, casing and cross gyration frame behind device base, equipment, device base upper end is connected with casing behind the equipment, and the casing outside is connected with the cross gyration frame behind the equipment, be connected with cross gyration inside casing on the cross gyration frame, reaction vessel cultivates the room is installed to cross gyration inside casing inboard, driving motor is installed to device base upper end, install inside casing driving pulley on the cross gyration frame, device base lower extreme is connected with the shock pad, reaction vessel cultivates the room upper end and is connected with the installation base, and is provided with the locating pin on the installation base to reaction vessel cultivates the room and installs on the cross gyration inside casing through installation base and locating pin, reaction vessel cultivates the room directly over at the device base simultaneously, be provided with reaction vessel cover on the reaction vessel cultivates the room, and reaction vessel covers to be stamped and to cover and have air exchange membrane, reaction vessel covers and be provided with luer, be provided with the base on the device base, and install through-hole connecting bolt on the base, the base outside is connected with mounting flange, be connected with first fixed screw on the base.

Preferably, the cross-shaped rotary outer frame is movably connected with the rear shell of the equipment, and the cross-shaped rotary outer frame forms a rotary structure on the rear shell of the equipment.

Preferably, the cross-shaped rotary inner frame is movably connected with the cross-shaped rotary outer frame, and the cross-shaped rotary inner frame forms a rotary structure on the cross-shaped rotary outer frame.

Preferably, the reaction vessel culture chamber and the mounting bosses are fixedly connected, and two mounting bosses are arranged on the reaction vessel culture chamber.

Preferably, the substrate base is provided with an isolation block, the outer side of the substrate base is connected with a second fixing screw, and the second fixing screw is connected with a special reaction vessel.

Preferably, the second fixing screw is connected with the substrate base in a threaded manner, and the second fixing screw forms a moving structure on the substrate base.

Compared with the prior art, the beneficial effects of the utility model are that: the two-axis three-dimensional rotary culture system;

(1) The product design, manufacture, sale and after sale based on the native can reduce the purchase cost of the user and improve the efficiency and quality of after-sale service.

(2) Redesigned the revolving bed pedestal, the external dimension reduces greatly, is fit for more application scenes, has increased quick location installation design to cross revolving frame inside casing simultaneously, has expanded its function.

(3) Redesigning a matched special culture container: spherical biological reaction vessels and matrix biological reaction vessels, and can provide better growth environment and microgravity effect simulation capability for cell culture by matching with special biological reaction vessels.

(4) The spherical biological reaction container and the matrix biological reaction container are internally provided with large-area air exchange membranes, which provide an environment far superior to the existing products for cell growth and simulate microgravity effect.

(5) The cross rotary frame is provided with a port for positioning and connection, and can be conveniently connected with a matched spherical biological reaction container or a matched matrix reaction container.

Drawings

FIG. 1 is a schematic view of the structure of the present invention;

FIG. 2 is a side view of the present invention;

FIG. 3 is a schematic view of the installation structure of the spherical bioreactor container of the present invention;

FIG. 4 is a schematic view of the installation structure of the matrix bioreactor vessel of the present invention;

FIG. 5 is a schematic view of the structure of the spherical bioreactor container of the present invention;

FIG. 6 is a schematic view of the structure of the matrix bioreactor vessel of the present invention;

FIG. 7 is a schematic diagram of the side view structure of the matrix bioreactor container of the present invention.

In the figure: 1. a device base; 2. a device rear housing; 3. a cross-shaped rotary outer frame; 4. a cross-shaped rotary inner frame; 5. a drive motor; 6. the inner frame drives the belt pulley; 7. a shock pad; 8. a reaction vessel culture chamber; 9. mounting a boss; 10. positioning pins; 11. a reaction vessel cover; 12. an air exchange membrane; 13. a luer; 14. a substrate holder; 15. the through hole is connected with a bolt; 16. installing a flange; 17. a first fixing screw; 18. an isolation block; 19. a second fixing screw; 20. a special reaction vessel.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-7, the present invention provides a technical solution: the utility model provides a two-axis three-dimensional gyration culture system, including device base 1, equipment rear shell 2, cross gyration frame 3, cross gyration inside casing 4, driving motor 5, inside casing drive pulley 6, shock pad 7, reaction vessel cultivates room 8, installation boss 9, the locating pin 10, reaction vessel lid 11, air exchange membrane 12, luer 13, base plate seat 14, through-hole connecting bolt 15, mounting flange 16, first fixed screw 17, the spacing block 18, second fixed screw 19 and special reaction vessel 20, device base 1 upper end is connected with equipment rear shell 2, and the equipment rear shell 2 outside is connected with cross gyration frame 3, be connected with cross gyration inside casing 4 on the cross gyration frame 3, reaction vessel cultivates room 8 is installed to cross gyration inside frame 4 inboard, device base 1 upper end container lid installs driving motor 5, install inside casing drive pulley 6 on the gyration outside frame 3, device base 1 lower extreme is connected with shock pad 7, reaction vessel cultivates room 8 upper end is connected with installation boss 9, and be provided with locating pin 10 on the installation boss 9, and reaction vessel cultivates room 8 and install container lid on container lid 4 through installation boss 9 and install container lid 10 on reaction vessel lid cover 4, it has 14 to be in the reaction vessel cultivates room 11 on the reaction vessel and is connected with reaction vessel base 13 on the reaction vessel base 13, and be provided with reaction vessel base connection flange 15 on the reaction vessel base 13, the reaction vessel base 13 is connected with the reaction vessel and be connected with the reaction vessel on the reaction vessel lid, the reaction vessel base 15, the reaction vessel base 13 is connected with the through-hole connecting bolt connection flange, and be connected with the reaction vessel on the reaction vessel lid, the reaction vessel base 14.

The connection mode of the cross rotary outer frame 3 and the equipment rear shell 2 is movably connected, the cross rotary outer frame 3 forms a rotating structure on the equipment rear shell 2, and the connection mode of the cross rotary outer frame 3 and the equipment rear shell 2 conveniently enables the cross rotary outer frame 3 to rotate on the equipment rear shell 2, so that the use is convenient.

The cross-shaped rotary inner frame 4 and the cross-shaped rotary outer frame 3 are movably connected, the cross-shaped rotary inner frame 4 forms a rotary structure on the cross-shaped rotary outer frame 3, the cross-shaped rotary inner frame 4 can rotate on the cross-shaped rotary outer frame 3 through the connection of the cross-shaped rotary inner frame 4 and the cross-shaped rotary outer frame 3, and therefore three-dimensional rotation can be achieved, and three-axis variable-speed constant-speed rotary motion can be achieved.

The connected mode of reaction vessel culture room 8 and installation boss 9 is fixed connection, and the number of installation boss 9 on reaction vessel culture room 8 is provided with two, reaction vessel culture room 8 makes reaction vessel culture room 8 more firm with being connected of installation boss 9 with the connected mode of installation boss 9, thereby prevent that it from taking place to drop, influence it and use, and the design of the number of installation boss 9, make it cultivate room 8 to reaction vessel better and install, thereby conveniently use.

The substrate base 14 is provided with a spacer 18, the outer side of the substrate base 14 is connected with a second fixing screw 19, and the substrate base 14 is provided with a special reaction vessel 20.

The second fixing screws 19 are connected with the substrate base 14 by screw threads, and the second fixing screws 19 form a moving structure on the substrate base 14, so that the substrate base 14 is convenient to mount.

The working principle is as follows: when the two-axis three-dimensional rotary culture system is used, firstly, the integrity of the device is maintained, then the device is transported to a corresponding working position and then used, after the driving motor 5 runs, the cross rotary frame is driven to rotate along the axis direction of the cross rotary frame through a belt, meanwhile, the inner frame driving belt wheel 6 is driven to move through internal gear transmission, and the inner frame driving belt wheel 6 drives the cross rotary inner frame 4 to rotate around the axis of the cross rotary inner frame. The biological reaction container for culturing cells and samples is arranged on the cross-shaped rotary inner frame 4 and moves synchronously with the inner frame, thereby realizing the rotary motion of the biological reaction container in the X \ Y \ Z three directions.

The reaction vessel culture chamber 8 can be conveniently mounted on the cross-shaped rotary inner frame 4 through the mounting bosses 9 and can move synchronously with the inner frame. The culture solution is injected into the reaction vessel from any luer 13 on the reaction vessel cover 11, and the luer 13 is closed after the bubbles inside the culture chamber 8 of the reaction vessel are discharged completely through any luer 13. In the process of growing samples such as cells, waste gas generated by metabolism and external air are exchanged through the air exchange membrane 12 to ensure the oxygen level inside the reaction vessel, the substrate base 14 is integrally installed in the same way as the culture chamber 8 of the ball reaction vessel, and can be quickly installed on the cross-shaped rotary inner frame 4 through the second fixing screws 19 and the positioning pins 10 and keep synchronous movement with the inner frame, and the contents which are not described in detail in the present specification, such as the air exchange membrane 12, are well known to those skilled in the art.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims

1. The utility model provides a two-axis three-dimensional gyration culture system, includes device base (1), casing (2) and cross gyration frame (3) behind equipment, its characterized in that: the device is characterized in that the upper end of a base (1) of the device is connected with a rear shell (2) of the device, the outer side of the rear shell (2) of the device is connected with a cross rotary outer frame (3), the cross rotary inner frame (4) is connected onto the cross rotary outer frame (3), a reaction vessel culture chamber (8) is installed on the inner side of the cross rotary inner frame (4), a driving motor (5) is installed at the upper end of the base (1), an inner frame driving belt wheel (6) is installed on the cross rotary outer frame (3), a damping pad (7) is connected to the lower end of the base (1), an installation boss (9) is connected onto the upper end of the reaction vessel culture chamber (8), a positioning pin (10) is arranged on the installation boss (9), the reaction vessel culture chamber (8) is installed on the rotary cross inner frame (4) through the installation boss (9) and the positioning pin (10), the reaction vessel culture chamber (8) is located right above the base (1), a reaction vessel cover (11) is arranged on the reaction vessel culture chamber (8), an air exchange membrane (12) is covered on the reaction vessel cover (11), a Ruhr interface (13) is arranged on the base (1), a base (14) of the device, and a through hole (15) is connected with a through bolt, the outer side of the substrate base (14) is connected with an installation flange (16), and the substrate base (14) is connected with a first fixing screw (17).

2. The two-axis three-dimensional rotating cultivation system according to claim 1, wherein: the cross rotary outer frame (3) is movably connected with the rear shell (2) of the equipment, and the cross rotary outer frame (3) forms a rotating structure on the rear shell (2) of the equipment.

3. The two-axis three-dimensional rotating cultivation system according to claim 1, wherein: the cross-shaped rotary inner frame (4) is movably connected with the cross-shaped rotary outer frame (3), and the cross-shaped rotary inner frame (4) forms a rotary structure on the cross-shaped rotary outer frame (3).

4. The biaxial three-dimensional rotation culture system according to claim 1, characterized in that: the reaction vessel culture chamber (8) is fixedly connected with the mounting bosses (9), and the number of the mounting bosses (9) on the reaction vessel culture chamber (8) is two.

5. The biaxial three-dimensional rotation culture system according to claim 1, characterized in that: the reactor is characterized in that an isolation block (18) is arranged on the substrate base (14), a second fixing screw (19) is connected to the outer side of the substrate base (14), and a special reaction container (20) is installed on the substrate base (14).

6. The two-axis three-dimensional rotating cultivation system according to claim 5, wherein: the second fixing screw (19) is connected with the substrate base (14) in a threaded manner, and the second fixing screw (19) forms a moving structure on the substrate base (14).