CN215517435U - A rabbling mechanism for microbial cultivation device - Google Patents

Abstract

The utility model relates to a stirring mechanism for a microorganism culture device, which comprises a stirrer and a driver for driving the stirrer to rotate, wherein the stirrer comprises a plurality of stirring components which are mutually matched, each stirring component comprises a stirring shaft, a plurality of stirring sheets formed at one end of the stirring shaft, a butting block arranged at the other end of the stirring shaft, a transmission gear arranged on the butting block and a driving block arranged on the transmission gear, and the driving block is connected with a main shaft of the driver. The stirring mechanism for the microorganism culture device correspondingly stirs the microorganisms in the culture dish through the stirring shaft and the stirring sheets in the stirring assembly, prevents the microorganisms in the culture dish from precipitating, enables nutrients and temperature in the culture dish to be uniformly distributed, and improves the microorganism culture efficiency.

Description

A rabbling mechanism for microbial cultivation device

Technical Field

The utility model relates to a technology of biochemical culture equipment, in particular to a stirring mechanism for a microorganism culture device.

Background

In the microbial culture test, the quality and content of the cultured cells or metabolites thereof are important indexes for determining the development of industries and enterprises, so the cell culture is the most core and basic technology in biological production. Microorganisms are often researched as model organisms due to the advantages of simple structure, rapid propagation, easy culture, easy variation and the like, so that the key is how to use a high-efficiency and sterile microbial culture technology and have accurate auxiliary equipment. In the research field, in order to improve the experimental efficiency, microorganisms are usually genetically modified at multiple sites at the same time, and the growth or metabolic status of the microorganisms is studied under various culture conditions.

The most common culture mode at present is to inoculate the cells into different shake bacteria tubes respectively and place in the constant temperature shaking incubator and cultivate, closely observe and regularly sample and measure its relevant parameter, and this process is very loaded down with trivial details from inoculation to every sampling operation, and is consuming time and power.

The cell culture plate reduces the workload to a certain extent, but is limited by the volume, the cell culture plate is placed in a constant-temperature shaking incubator for shaking culture, the strain is easy to precipitate, the cell culture plate is mostly placed in the constant-temperature incubator for static culture at present, but nutrients, temperature and oxygen cannot be uniformly distributed in each area in the culture plate, and the growth efficiency of the cells is greatly limited.

Disclosure of Invention

The utility model provides a stirring mechanism for a microorganism culture device, which can prevent microorganisms from precipitating and improve the microorganism culture efficiency.

In order to achieve the purpose, the technical scheme of the utility model is as follows: the utility model provides an rabbling mechanism for microorganism culture apparatus for the microorganism in the stirring culture dish, includes the agitator and orders about agitator pivoted driver, this agitator includes a plurality of stirring subassemblies that interact, and each stirring subassembly includes the (mixing) shaft, forms at a plurality of stirring pieces of (mixing) shaft one end, sets up at the (mixing) shaft other end support hold the piece, set up support the drive gear on holding the piece and set up the drive block on drive gear, the drive block with the main shaft of driver is connected.

As a further improvement, the transmission gears in every two adjacent stirring assemblies are mutually meshed so as to rotate through one stirring assembly, and then drive the other stirring assemblies to rotate.

As a further improvement, the driving block is provided with a clamping hole, one end of a rotating shaft of the driver is clamped in the clamping hole of the driving block in one of the stirring assemblies, the driving block is driven to rotate by the driver, the transmission gear corresponding to the lower part of the driving block rotates to drive the stirring shaft and the stirring sheet formed at one end of the stirring shaft to rotate, and then other transmission gears meshed with each other are driven to rotate, so that the stirring shafts of all the stirring assemblies and the stirring sheets formed at one end of each stirring shaft rotate, and the microorganisms in the culture dish are stirred and cultured simultaneously.

As further improvement, still include the machine box, the machine box includes box body and the last box body of setting on box body under, and the bottom of box body supports and leans on the window department to a shell down, forms a installation cavity between lower box body and the last box body, for the installation the agitator, a plurality of through-holes have been seted up uniformly to the bottom of lower box body to the (mixing) shaft that corresponds the penetrating agitator.

As a further improvement, the stirring assemblies are 12 and are linearly arrayed in three rows and four columns at the installation cavity of the machine box.

As a further improvement, each stirring shaft can rotatably penetrate through a through hole at the bottom of the lower box body, and a plurality of stirring blades are formed at one end of the stirring shaft arranged outside the box body.

As a further improvement, the end part of the stirring shaft arranged in the machine box is provided with the abutting block, and the abutting block is contacted with the inner peripheral bottom surface of the lower box body.

As a further improvement, the stirring plates are three and are obliquely and annularly arrayed on the outer axial surface of the stirring shaft by taking the axis of the stirring shaft as a central line.

As a further improvement, the stirring shaft is a cone-shaped structure stirring shaft made of zirconia all-ceramic.

As a further improvement, the driver is electrically connected with a control mechanism through a lead.

The technical scheme of the utility model has the following beneficial effects: the stirring mechanism for the microorganism culture device correspondingly stirs the microorganisms in the culture dish through the stirring shaft and the stirring sheets in the stirring assembly, prevents the microorganisms in the culture dish from precipitating, enables nutrients and temperature in the culture dish to be uniformly distributed, and improves the microorganism culture efficiency.

Drawings

FIG. 1 is a schematic view showing the structure of a microorganism culture apparatus according to the present invention.

Fig. 2 is a schematic view of fig. 1 from another perspective.

Fig. 3 is a schematic view of the structure of fig. 1.

FIG. 4 is a schematic view showing the structure of the opened bottom wall 12 of the microorganism culture apparatus of the present invention.

FIG. 5 is a schematic view showing the structure of the opened casing 23 of the microorganism culture apparatus of the present invention.

FIG. 6 is a schematic view showing the structure of the opened upper case 262 of the microorganism culture apparatus of the present invention.

FIG. 7 is a schematic structural view of a stirring member 271 of the microorganism culture apparatus of the present invention.

Detailed Description

The embodiments of the present invention will be described in detail below with reference to the drawings and examples.

Referring to fig. 1 to 3, a microorganism culture apparatus according to the present invention for efficient, fast and non-interfering cultivation of microorganisms includes a base 10, a body 20 disposed on the base 10, and a carrying structure 30 disposed between the base 10 and the body 20 for carrying microorganisms.

Be equipped with control mechanism 11 on pedestal 10 for control organism 20 and the motion of carrying thing structure 30, and control organism 20 stirs the microorganism that carries thing structure 30 and bear, prevent that the microorganism from deposiing, improve the evenly distributed performance of medium such as nutrient, temperature in carrying thing structure 30, pedestal 10 includes diapire 12, form lateral wall 13 and the roof 14 of setting on lateral wall 13 around diapire 12, form a cavity between lateral wall 13 and the roof 14, for installation control mechanism 11.

Referring to fig. 4, the control mechanism 11 includes a main housing 110, and a rotation button 111, a forward/reverse button 112, an up button 113 and a down button 114 electrically connected to the main housing 110. The main chassis 110 is disposed in the cavity of the housing 10 and is mounted on the inner peripheral surface of the top wall 14, and the rotation speed button 111, the forward/reverse rotation button 112, the ascending button 113 and the descending button 114 are mounted on one of the side walls 13 of the housing 10 and are disposed on the outer peripheral surface of the side wall 13, in this embodiment, each control button is mounted on the side wall 13 at the front end of the housing 10. Be equipped with circuit board and battery in the mainframe box 110, be connected with the power cord on the mainframe box 110 to supply with the power connection, the circuit board corresponds ground and organism 20 electric connection through a plurality of wires, with the motion state through the control button control organism 20 that corresponds and carry thing structure 30, the battery can be under the outage state to the device power supply, in order to last continuation of the journey work. The side wall 13 opposite to the side wall where the control button is installed is provided with a wiring groove 131 for a wire to pass through. In this embodiment, the sidewall 13 with the wiring groove 131 is the sidewall 13 at the rear end of the base 10.

Referring to fig. 4, a wiring block 15 and a first limit switch 16 are disposed in the cavity of the base 10, and a wiring hole 151 is disposed on the wiring block 15, so that a plurality of wires correspondingly pass through the wiring hole and are neatly arranged in the cavity of the base 10 through the wiring block 15; the first limit switch 16 is used to control the descending distance of the loading structure 30, so as to prevent the loading structure 30 from touching the base 10. Specifically, the wiring block 15 and the limit switch 16 are mounted on the inner peripheral surface of the top wall 14.

Referring to fig. 4 to 6, the body 20 is installed on the periphery of the sidewall 13 of the seat 10 with the wiring groove 131, the body 20 includes a supporting plate 21 and a host 22 installed on the supporting plate 21, one end of the supporting plate 21 is connected to the sidewall 13 of the seat 10, and the other end of the supporting plate 21 is connected to the host 22. A wire passing hole (not shown) is formed in the supporting plate 21 for a wire passing through the wiring groove 131 to pass through the supporting plate 21 along the wire passing hole and electrically connected to the host 22.

The main unit 22 includes a casing 23, a second limit switch 29 disposed in the casing 23, and an agitating mechanism 24 and a transmission mechanism 25 disposed on the casing 23, wherein the agitating mechanism 24 is used for agitating the microorganisms carried by the carrying structure 30, preventing the microorganisms from settling, and improving the uniform distribution performance of nutrients and temperature in the carrying structure 30. The transmission mechanism 25 is used to drive the loading structure 30 to move up and down between the base 10 and the machine body 20.

The housing 23 includes a bottom 231, a side 232 formed around the bottom 231, and a top 233 disposed on the side 232, wherein a receiving cavity is formed among the bottom 231, the side 232, and the top 233 for mounting the stirring mechanism 24 and the transmission mechanism 25, and the bottom 231 is opened with a window for the stirring mechanism 24 to penetrate.

The stirring mechanism 24 includes a housing 26, a stirrer 27 disposed in the housing 26, and a driver 28 for driving the stirrer 27 to rotate. The casing 26 is fixedly disposed in the accommodating cavity of the housing 23, the casing 26 includes a lower casing 261 and an upper casing 262 disposed on the lower casing 261, and a bottom of the lower casing 261 abuts against a window of the bottom 231 of the housing 23. An installation cavity is formed between the lower box body 261 and the upper box body 262 for installing the stirrer 27, and a plurality of through holes are uniformly formed in the bottom of the lower box body 261 to correspondingly penetrate the stirrer 27.

Referring to fig. 7, the stirrer 27 includes a plurality of stirring assemblies 271 cooperating with each other, each stirring assembly 271 includes a stirring shaft 2711, a plurality of stirring blades 2712 formed at one end of the stirring shaft 2711, a holding block 2713 disposed at the other end of the stirring shaft 2711, a transmission gear 2714 disposed on the holding block 2713, and a driving block 2715 disposed on the transmission gear 2714, wherein the driving block 2715 is provided with a holding hole 2716 for fitting the driver 28. Specifically, the stirring assemblies 271 are 12 and are linearly arrayed in three rows and four columns at the installation cavity of the machine box 26.

Each stirring shaft 2711 rotatably penetrates through a through hole at the bottom of the lower box body 261, one end of the stirring shaft 2711 arranged outside the box 26 takes the axis of the stirring shaft 2711 as a central line, and is provided with a plurality of stirring blades 2712 in an annular array, and the end part of the stirring shaft 2711 arranged inside the box 26 is provided with a supporting block 2713, so that the supporting block 2713 is supported against the inner peripheral bottom surface of the lower box body 261. Referring to fig. 6, the transmission gears 2714 of two adjacent stirring assemblies 271 are engaged with each other, so that one stirring assembly 271 rotates to drive the other stirring assemblies 271 to rotate. The stirring shaft 2711 is a conical stirring shaft made of zirconia all-ceramic, so that high-precision, smooth, wear-resistant and corrosion-resistant effects are realized in the process of stirring microorganisms, and the stirring shaft 2711 is convenient to replace.

The driver 28 is a driving motor, and the driving motor is electrically connected with the rotating speed button 111 and the forward and backward rotation button 112 of the control mechanism 11 through wires, so as to adjust the rotating speed and the rotation direction of the driving motor, further control the stirring state of the stirrer 27 on the microorganisms, prevent the microorganisms from precipitating, and enable the microorganism culture effect to reach the best. One end of the rotating shaft of the driving motor is clamped in a clamping hole 2716 of a driving block 2715 in one of the stirring assemblies 271, and is rotated by the driving motor, one of the driving blocks 2715 rotates, corresponding to the rotation of the transmission gear 2714 at the lower portion of the driving block 2715, and drives the stirring shaft 2711 and the stirring piece 2712 formed at one end of the stirring shaft 2711 to rotate, and the stirring piece 2714 rotates through one transmission gear, thereby driving other transmission gears 2714 engaged with each other to rotate, so that the stirring shafts 2711 of the 12 stirring assemblies 271 and the stirring blades 2712 formed at one end of each stirring shaft 2711 rotate, so as to realize the simultaneous stirring of the microorganisms carried by the carrying structure 30, the other end of the rotating shaft of the driving motor passes through the top 233 of the casing 23, and a driving motor protecting cover 281 is sleeved at the rotating shaft of the driving motor penetrating through the top 233 of the casing 23 to prevent the rotating shaft of the driving motor from falling into dust and improve the aesthetic property of the whole structure.

Referring to fig. 5 to 6, the transmission mechanism 25 is installed on the housing 23 for driving the loading structure 30 to move up and down between the base 10 and the body 20, and specifically, the transmission mechanism 25 includes a rotating motor 251, a rotating assembly 252 connected to one end of a rotating shaft of the rotating motor 251, and guide rods 253 disposed on two sides of the rotating assembly 252. The rotating motor 251 is installed in the housing 23 and electrically connected to the ascending button 113 and the descending button 114 of the control mechanism 11 through wires, so as to adjust the rotation direction of the rotating motor 251, and further control the ascending or descending of the carrying structure 30. The other end of the rotating shaft of the rotating motor 251 penetrates the top 233 of the casing 23, and the rotating shaft of the rotating motor 251 penetrating the top 233 of the casing 23 is sleeved with a rotating motor shield 2511, and the rotating motor shield 2511 is arranged outside the top 233 of the casing 23 to prevent the rotating shaft of the rotating motor 251 from falling into dust and improve the aesthetic property of the whole structure.

The rotating assembly 252 includes a screw 2522, a shaft sleeve 2521 mounted at one end of the screw 2522, and a rotating base 2523 mounted at the other end of the screw 2522, the shaft sleeve 2521 is mounted at the bottom 231 of the housing 23 for the rotating shaft of the rotating motor 251 to penetrate through, one end of the screw 2522 penetrates through the shaft sleeve 2521 to be connected with the rotating shaft of the rotating motor 251, the other end of the screw 2522 penetrates through the top wall 14 of the housing 10 and is connected with the rotating base 2523, the rotating base 2523 is disposed in the cavity of the housing 10 and is mounted at the inner circumferential surface of the top wall 14, the overall structure of the rotating assembly 252 is configured to improve the rotating stability thereof, thereby improving the stability of the up-and-down movement of the object carrying structure 30 between the housing 10 and the housing 20.

Two guide rods 253 are correspondingly arranged at two sides of the rotating assembly 252, one end of each guide rod 253 is installed at the bottom 231 of the housing 23, and the other end of each guide rod 253 is installed at the top wall 14 of the seat body 10, so as to further improve the stability of the up-and-down movement of the loading structure 30 between the seat body 10 and the machine body 20.

The second limit switch 29 is disposed in the casing 23 and on one of the side portions 232 for controlling the ascending distance of the loading structure 30 and preventing the loading structure 30 from touching the machine body 20. The first limit switch 16 and the second limit switch 29 correspondingly control the positive and negative rotation speeds of the rotating motor 251 to improve the use safety.

Referring to fig. 5 to 6 again, the carrying structure 30 is disposed between the base 10 and the body 20 in a lifting manner for carrying the microorganism, and cooperates with the stirrer 27 to stir and culture the microorganism. Specifically, the loading structure 30 includes a lifting platform 31 and a loading platform 32 disposed on the lifting platform 31. One end of the lifting platform 31 is correspondingly provided with a screw hole and guide holes disposed at two sides of the screw hole, an internal thread is disposed in the screw hole for the screw rod 2522 to pass through the screw hole and to be engaged with the internal thread of the screw hole, a guide rod 253 is correspondingly disposed in each guide hole, the rotating motor 251 of the transmission mechanism 25 drives the screw rod 2522 of the rotating assembly 252 to rotate, and the lifting platform 31 is lifted stably along the screw rod 2522 and the guide rod 253.

A holding groove is formed on the table top of the lifting platform 31 for holding the mounting object carrying platform 32, the object carrying platform 32 comprises a mounting plate 321 and an object carrying plate 322 formed on the mounting plate 321, the object carrying platform 32 is detachably mounted in the holding groove of the table top of the lifting platform 31 through the mounting plate 321, a plurality of object carrying cavities 3221 are uniformly arranged on the object carrying plate 322, each object carrying cavity 3221 forms a culture dish for carrying microorganisms, and each object carrying cavity 3221 corresponds to a stirring component 271, so as to stir the microorganisms in the object carrying cavity 3221, thereby preventing the microorganisms from settling. In this embodiment, there are 12 loading cavities 3221, and they are linearly arranged in three rows and four columns on loading board 322.

During work, the object carrying platform 32 is detached along the clamping groove of the table top of the lifting platform 31 through the mounting plate 321, microorganisms are placed in the culture dish of the object carrying platform 32, and then the object carrying platform 32 is mounted in the clamping groove of the table top of the lifting platform 31 through the mounting plate 321; when the ascending button 113 and the descending button 114 of the control mechanism 11 are pressed, the rotating motor 251 of the transmission mechanism 25 is driven to rotate, the lead screw 2522 of the rotating assembly 252 is driven to rotate, and the lifting platform 31 is lifted up and down smoothly along the lead screw 2522 and the guide rod 253, so that the stirring shaft 2711 and the stirring blades 2712 of the stirring assembly 271 are placed into the microorganism in the culture dish of the object carrying platform 32; the rotating speed button 111 and the forward and reverse rotating buttons 112 of the control mechanism 11 are pressed to adjust the rotating speed and the rotating direction of the driver 28, so as to control the stirring shafts 2711 of the stirring assemblies 271 in the stirrer 27 and the stirring state of the microorganisms in the culture dish by the stirring blades 2712 formed at one end of each stirring shaft 2711, thereby preventing the microorganisms in the culture dish from precipitating and enabling the microorganism culture effect to reach the best.

In summary, the microorganism culture apparatus of the present invention controls the stirring state of each stirring assembly 271 of the stirrer 27 for the microorganisms in the culture dish by adjusting the rotation speed and the rotation direction of the driver 28, so as to prevent the microorganisms in the culture dish from precipitating, and uniformly distribute the nutrients and the temperature in the culture dish, and by adjusting the elevation of the object carrying structure 30, the microorganisms in each culture dish of the object carrying platform 32 individually correspond to one stirring assembly 271, and the stirring assemblies 271 independently stir the microorganisms in the culture dish, thereby ensuring that the microorganism culture process has no mixed bacteria interference, and improving the microorganism culture efficiency.

The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solution of the present invention by those skilled in the art should fall within the protection scope defined by the claims of the present invention without departing from the spirit of the present invention.

Claims (10)

1. The utility model provides a rabbling mechanism for microbial cultivation device for the microorganism in the stirring culture dish, characterized in that, including agitator (27) and drive agitator (27) pivoted driver (28), this agitator (27) include a plurality of stirring subassembly (271) of mutually supporting effect, each stirring subassembly (271) includes (mixing) shaft (2711), form a plurality of stirring piece (2712) in (mixing) shaft (2711) one end, set up support piece (2713) at (mixing) shaft (2711) other end, set up drive gear (2714) on support piece (2713) and set up drive block (2715) on drive gear (2714), drive block (2715) with the main shaft of driver (28) is connected.

2. The stirring mechanism for a microorganism culture apparatus according to claim 1, wherein: the transmission gears (2714) in every two adjacent stirring assemblies (271) are mutually meshed so as to rotate through one stirring assembly (271) and further drive other stirring assemblies (271) to rotate.

3. The stirring mechanism for a microorganism culture apparatus according to claim 2, wherein: the culture dish is characterized in that a clamping hole (2716) is formed in the driving block (2715), one end of a rotating shaft of the driver (28) is clamped in the clamping hole (2716) of the driving block (2715) in one of the stirring assemblies (271), the driver (28) drives the driving block (2715) to rotate, and a transmission gear (2714) corresponding to the lower part of the driving block (2715) rotates to drive the stirring shaft (2711) and a stirring piece (2712) formed at one end of the stirring shaft (2711) to rotate, so that other transmission gears (2714) meshed with each other are driven to rotate, the stirring shafts (2711) of all the stirring assemblies (271) and the stirring pieces (2712) formed at one end of each stirring shaft (2711) rotate, and the culture dish is stirred by microorganisms in the culture dish.

4. The stirring mechanism for a microorganism culture apparatus according to claim 1, wherein: still include machine box (26), this machine box (26) including box body (261) down and set up last box body (262) on box body (261) down, the bottom of lower box body (261) supports and leans on to the window department of a casing (23), forms a installation cavity between lower box body (261) and last box body (262), in order to supply the installation agitator (27), a plurality of through-holes have been seted up uniformly to the bottom of lower box body (261) to correspond (mixing) shaft (2711) that penetrates agitator (27).

5. The stirring mechanism for a microorganism culture apparatus according to claim 4, wherein: the number of the stirring assemblies (271) is 12, and the stirring assemblies are arranged in a linear array of three rows and four columns at the installation cavity of the machine box (26).

6. The stirring mechanism for a microorganism culture apparatus according to claim 4, wherein: each stirring shaft (2711) can rotatably penetrate through a through hole at the bottom of the lower box body (261), and a plurality of stirring blades (2712) are formed at one end of the stirring shaft (2711) arranged outside the machine box (26).

7. The stirring mechanism for a microorganism culture apparatus according to claim 4, wherein: the abutting block (2713) is arranged at the end part of the stirring shaft (2711) arranged in the machine box (26), and the abutting block (2713) is contacted with the inner peripheral bottom surface of the lower box body (261).

8. The stirring mechanism for a microorganism culture apparatus according to claim 6, wherein: the stirring blades (2712) are three, and are obliquely and annularly arrayed on the outer axial surface of the stirring shaft (2711) by taking the axis of the stirring shaft (2711) as a central line.

9. The stirring mechanism for a microorganism culture apparatus according to claim 1, wherein: the stirring shaft (2711) is a conical stirring shaft made of zirconia all-ceramic.

10. The stirring mechanism for a microorganism culture apparatus according to claim 1, wherein: the driver (28) is electrically connected with a control mechanism (11) through a lead.

Images