CN107435023A - A kind of bioreactor and biologic reaction equipment - Google Patents
A kind of bioreactor and biologic reaction equipment Download PDFInfo
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- CN107435023A CN107435023A CN201710855964.8A CN201710855964A CN107435023A CN 107435023 A CN107435023 A CN 107435023A CN 201710855964 A CN201710855964 A CN 201710855964A CN 107435023 A CN107435023 A CN 107435023A
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- stirrer
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- culture plate
- driver
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- 238000006243 chemical reaction Methods 0.000 title claims abstract description 11
- 239000000853 adhesive Substances 0.000 claims abstract description 70
- 230000001070 adhesive effect Effects 0.000 claims abstract description 70
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 39
- 238000009826 distribution Methods 0.000 claims description 6
- 230000005540 biological transmission Effects 0.000 claims description 5
- 230000001154 acute effect Effects 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 abstract description 25
- 238000012258 culturing Methods 0.000 abstract description 2
- 230000001954 sterilising effect Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000005299 abrasion Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- -1 polytetrafluoroethylene Polymers 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000005476 soldering Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a kind of bioreactor and biologic reaction equipment, belongs to organism culturing device field.Bioreactor includes driver, culture plate and stirrer.The driver is provided with the first adhesive part, and the driver can drive the first adhesive part to rotate.Culture plate is positioned on the driver, and stirrer is positioned in culture plate.Stirrer includes the second adhesive part, and the second adhesive part is connected with the first adhesive part magnetic.Connected between driver and stirrer using contactless magnetic, when driver drives the first adhesive part to rotate, in the presence of magnetic force, stirrer will rotate in culture plate, so as to be stirred to the culture medium in culture plate.When needing to observe the culture medium in culture plate, directly culture plate can be removed and be observed, without to stirrer is taken out from culture plate, operation is extremely convenient, and culture medium will not be polluted.
Description
Technical field
The present invention relates to organism culturing device field, in particular to a kind of bioreactor and biologic reaction equipment.
Background technology
Bioreactor refers to the engineering equipment for providing bioactivity environment, and in the prior art, bioreactor typically wraps
Container, driver and stirrer are included, stirrer is extended in container at the top of container, and stirrer is fixed on a drive, is driven
Dynamic device work directly will drive stirrer to rotate, so as to be stirred to the culture medium in container.In this bioreactor
Driver is joined directly together with stirrer, needs to remove stirrer and driver when the culture medium in container is observed,
Operation is extremely inconvenient, while significantly increases the contamination probability of culture medium inside container.
The content of the invention
It is an object of the invention to provide a kind of bioreactor, operation inconvenience when being observed with improving culture medium,
The problem of easily causing pollution.
It is an object of the invention to provide a kind of biologic reaction equipment, is operated not when being observed with improving culture medium
Just the problem of pollution, is easily caused.
What the present invention was realized in：
Based on above-mentioned first purpose, the present invention provides a kind of bioreactor, including driver, culture plate and stirrer；
Driver is provided with the first adhesive part, and driver can drive the first adhesive part to rotate；
Culture plate is positioned on driver, and stirrer is positioned in culture plate；
Stirrer includes the second adhesive part, and the second adhesive part is connected with the first adhesive part magnetic.
Further, culture plate is internally formed multiple open-topped culture lattice, and the first adhesive part is with cultivating lattice 1 a pair
Should, stirrer is positioned in culture lattice.
Further, the distribution of lattice rectangular array is cultivated.
Further, driver includes pedestal and force piece, and the first adhesive part is rotatably arranged at pedestal, and culture plate is put
It is placed on pedestal, force piece is used to drive the first adhesive part opposite base to rotate.
Further, pedestal includes at least one pedestal, and culture plate is positioned on pedestal, a pair of culture plate and pedestal 1
Should, pedestal is provided with multiple the first adhesive parts be arrangeding in parallel, and force piece can drive all first adhesive parts in pedestal to rotate.
Further, two the first adjacent adhesive parts in pedestal are connected by transmission component.
Further, stirrer also includes at least two blades agitating shaft and circle distribution on the outside of agitating shaft, and second
Adhesive part is fixed on agitating shaft；
Plane where blade is reference planes, and the axis of agitating shaft is axis of reference, and reference planes are in axis of reference
Angle is set, and reference planes and reference axis are in that angle is acute angle.
Further, the inside of agitating shaft is provided with closed chamber, and the second adhesive part is in closed chamber.
Further, stirrer also includes wearing piece, and wearing piece is fixed on the bottom of agitating shaft.
Based on above-mentioned second purpose, the present invention provides a kind of biologic reaction equipment, including controller and above-mentioned biological respinse
Device, controller are used to control driver.
The beneficial effects of the invention are as follows：
The present invention provides a kind of bioreactor, is connected between driver and stirrer using contactless magnetic, when
When driver drives the rotation of the first adhesive part, in the presence of magnetic force, stirrer will rotate in culture plate, so as to culture plate
In culture medium be stirred.When needing to observe the culture medium in culture plate, culture plate directly can be removed into progress
Observation, without to stirrer is taken out from culture plate, operation is extremely convenient, will not be polluted to culture medium.This biology
Reactor can effectively reduce cumbersome assembling during sterilization and close time, only be needed to stirrer in sterilization process
Individually sterilizing；In addition, also easily stirrer can be changed.
The present invention provides a kind of biologic reaction equipment, including controller and above-mentioned bioreactor, has above-mentioned biology anti-
Answer all advantages of device.Controller can be controlled to driver, so as to realize that the rotating speed to stirrer is controlled, for experiment
Repeatability offer precise data guarantee.
Brief description of the drawings
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below by embodiment it is required use it is attached
Figure is briefly described, it will be appreciated that the following drawings illustrate only certain embodiments of the present invention, therefore be not construed as pair
The restriction of scope, for those of ordinary skill in the art, on the premise of not paying creative work, can also be according to this
A little accompanying drawings obtain other related accompanying drawings.
Fig. 1 is the structural representation for the bioreactor that the embodiment of the present invention 1 provides；
Fig. 2 is the structural representation of the driver shown in Fig. 1；
Fig. 3 is the structural representation of the pedestal shown in Fig. 2；
Fig. 4 is the structural representation of the first adhesive part shown in Fig. 2；
Fig. 5 is the structural representation of the culture plate shown in Fig. 1；
Fig. 6 is the structural representation of the stirrer shown in Fig. 1；
Fig. 7 is the sectional view of the stirrer shown in Fig. 1；
Fig. 8 is the sectional view of the first semiaxis shown in Fig. 7；
Fig. 9 is the structural representation of the first semiaxis shown in Fig. 7；
Figure 10 is the structural representation of the second semiaxis shown in Fig. 7；
Figure 11 is the structural representation for the biologic reaction equipment that the embodiment of the present invention 2 provides.
Icon：100- bioreactors；10- drivers；11- pedestals；111- pedestals；1111- bottom plates；1112- support members；
1113- protuberances；1114- blind holes；112- electric machine supports；12- force pieces；13- the first adhesive parts；131- rotating shafts；132- magnetic
Part；14- transmission components；141- gears；15- output gears；20- culture plates；21- cultivates lattice；30- stirrers；31- agitating shafts；
311- closed chambers；The semiaxis of 312- first；3121- first end faces；The joint faces of 3122- first；The blind holes of 3123- first；313- second
Semiaxis；3131- second end faces；The joint faces of 3132- second；3133- is raised；The blind holes of 3134- second；32- the second adhesive parts；33-
Wearing piece；34- blades；35- reference planes；36- axiss of reference；200- biologic reaction equipments；210- controllers.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
Part of the embodiment of the present invention, rather than whole embodiments.The present invention implementation being generally described and illustrated herein in the accompanying drawings
The component of example can be configured to arrange and design with a variety of.
Therefore, below the detailed description of the embodiments of the invention to providing in the accompanying drawings be not intended to limit it is claimed
The scope of the present invention, but be merely representative of the present invention selected embodiment.It is common based on the embodiment in the present invention, this area
The every other embodiment that technical staff is obtained under the premise of creative work is not made, belong to the model that the present invention protects
Enclose.
It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in the present invention can phase
Mutually combination.
It should be noted that：Similar label and letter represents similar terms in following accompanying drawing, therefore, once a certain Xiang Yi
It is defined, then it further need not be defined and explained in subsequent accompanying drawing in individual accompanying drawing.
, it is necessary to which explanation, indicating position or position relationship are based on shown in accompanying drawing in the description of the embodiment of the present invention
Orientation or position relationship, or the invention product using when the orientation usually put or position relationship, or this area
The orientation or position relationship that technical staff usually understands, or the invention product using when the orientation usually put or position close
System, it is for only for ease of and describes the present invention and simplify description, rather than indicates or imply that signified device or element must have
Specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.In addition, term " the
One ", " second " is only used for distinguishing description, and it is not intended that instruction or hint relative importance.
In the description of the embodiment of the present invention, it is also necessary to explanation, unless otherwise clearly defined and limited, term
" setting ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected, or integratedly be connected
Connect；Can be directly connected to, can also be indirectly connected with by intermediary.For the ordinary skill in the art, may be used
The concrete meaning of above-mentioned term in the present invention is understood with concrete condition.
Embodiment 1
As shown in figure 1, the present embodiment provides a kind of bioreactor 100, including driver 10, culture plate 20 and stirrer
30, culture plate 20 is placed on driver 10, and stirrer 30 is placed in culture plate 20, and driver 10 is in a manner of magnetically-actuated
Driving stirrer 30 rotates.
Wherein, as shown in Fig. 2 driver 10 includes pedestal 11 and force piece 12, rotatably it is provided with pedestal 11 more
Individual first adhesive part 13, force piece 12 are used to drive the opposite base 11 of the first adhesive part 13 to rotate.
Specifically, pedestal 11 includes electric machine support 112 and two pedestals 111, and two pedestals 111 pass through electric machine support 112
Connection, two pedestals 111 are symmetrically distributed in the both sides of electric machine support 112.
As shown in figure 3, pedestal 111 includes bottom plate 1111 and four support members 1112 on the upper surface of bottom plate 1111,
Bottom plate 1111 is rectangle, and four support members 1112 are respectively arranged at the corner of bottom plate 1111, are formed between four support members 1112
The clamping section of one rectangle.Support member 1112 is L-shaped, and the inner side of support member 1112 is provided with protuberance 1113, four support members
In the upper surface of the protuberance 1113 of 1112 inner sides is generally aligned in the same plane.Bottom plate 1111 is provided with the multiple blind of rectangular array distribution
Hole 1114.
In the present embodiment, force piece 12 is motor.
As shown in figure 4, the first adhesive part 13 includes rotating shaft 131 and the magnetic part 132 in rotating shaft 131, magnetic part 132
It is cylinder with rotating shaft 131, magnetic part 132 is coaxially disposed with rotating shaft 131, the diameter of magnetic part 132 and the diameter of rotating shaft 131
Equal, the diameter of rotating shaft 131 and the aperture of the blind hole 1114 on bottom plate 1111 match, and magnetic part 132 is fixed on rotating shaft 131
One end.In the present embodiment, magnetic part 132 is magnet.
As shown in Fig. 2 it is correspondingly arranged a first adhesive part 13, the first adhesive in a blind hole 1114 on pedestal 111
The one end of rotating shaft 131 away from magnetic part 132 in part 13 is inserted in blind hole 1114, the magnetic part in the first adhesive part 13 132
In outside blind hole 1114.After first adhesive part 13 is in blind hole 1114, the first adhesive part 13 can rotate relative to pedestal 111, and first
Adhesive part 13 is the first adhesive part 13 axis of itself relative to the axis that pedestal 111 rotates.Any two in pedestal 111
It is parallel to each other between one adhesive part 13.Two the first adjacent adhesive parts 13 in pedestal 111 are connected by transmission component 14.This
In embodiment, transmission component 14 is two pitch wheels 141, and two meshing gears 141 are corresponding therewith with two respectively
The first adhesive part 13 fix.Motor is arranged vertically on electric machine support 112, and the output shaft of motor is provided with output gear 15,
The gear 141 on a first adhesive part 13 in one pedestal 111 engages with output gear 15, in another pedestal 111
Gear 141 on one the first adhesive part 13 engages with output gear 15.When motor works, all first in pedestal 11 inhale
Component 13 is all by the axis rotation around itself.
Culture plate 20 corresponds with pedestal 111, i.e., culture plate 20 is also two.Culture plate 20 is plastic material, culture
Disk 20 is rectangular configuration.What is formed between the size of culture plate 20 and four support members 1112 of pedestal 111 clamps the big of section
It is small consistent.As shown in figure 5, culture plate 20 is internally formed multiple open-topped culture lattice 21, culture lattice 21 rectangular array is distributed,
Lattice 21 are cultivated as cylinder.The distance between two adjacent culture lattice 21 in culture plate 20 and two phases in pedestal 111
The distance between the first adjacent adhesive part 13 is equal.
As shown in fig. 6, stirrer 30 includes agitating shaft 31, the second adhesive part 32, wearing piece 33 and circle distribution in stirring
Three blades 34 in the outside of axle 31, the second adhesive part 32 and wearing piece 33 are on agitating shaft 31.
Wherein, agitating shaft 31 is plastic material, its lighter weight.As shown in fig. 7, agitating shaft 31 is generally cylindrical, stir
Mix axle 31 has two end faces, respectively first end face 3121 and second end face 3131, the He of first end face 3121 in its axial direction
Second end face 3131 is plane, and first end face 3121 is the top of agitating shaft 31, and second end face 3131 is the bottom of agitating shaft 31
Portion.The inside of agitating shaft 31 is provided with a closed chamber 311.
Specifically, agitating shaft 31 includes dismountable two parts, i.e. the first semiaxis 312 and the second semiaxis 313 up and down, and first
The semiaxis 313 of semiaxis 312 and second is cylinder, and the external diameter of the first semiaxis 312 is equal with the external diameter of the second semiaxis 313.First
The semiaxis 313 of semiaxis 312 and second divides equally agitating shaft 31, i.e. the length of the first semiaxis 312 and the length phase of the second semiaxis 313
Deng.First semiaxis 312 is coaxially disposed with the second semiaxis 313, and the first semiaxis 312 and the second semiaxis 313 are removably attachable to one
Rise.
Wherein, the first semiaxis 312 forms the first accommodating chamber of one end open.As shown in figure 8, the first semiaxis 312 is in its axle
There are two end faces upwards, one of end face is the first end face 3121 of agitating shaft 31, and another end face is the first joint face
3122.The first blind hole 3123 is offered on first joint face 3122, the first blind hole 3123 is coaxially disposed with the first semiaxis 312, the
One blind hole 3123 is the first accommodating chamber.
In the present embodiment, three blades 34 are on the first semiaxis 312.Three circumference of blade 34 are distributed in the first semiaxis
312 outside, three uniform intervals of blade 34 distributions, i.e., each blade 34 is at a distance of 120 degree.
Blade 34 is stripe board, and the material of blade 34 is identical with the material of agitating shaft 31.As shown in figure 9, blade 34 is along
One semiaxis 312 radially extends, i.e., the length direction of blade 34 is consistent with the radial direction of the first semiaxis 312.As shown in figure 8,
Blade 34 is obliquely installed with respect to the first semiaxis 312.That is, the plane where blade 34 is reference planes 35, the first semiaxis
312 axis is axis of reference 36, and reference planes 35 are set with axis of reference 36 in angle, reference planes 35 and axis of reference 36
Be in angle is acute angle.In the present embodiment, reference planes 35 and axis of reference 36 are in that angle is 30 degree.
In actual production process, blade 34 can be integrally formed with the first semiaxis 312 by way of injection molding, also may be used
Both are separately manufactured, then be attached by way of sweat soldering.
Second semiaxis 313 forms the second accommodating chamber of one end open.As shown in Figure 10, the second semiaxis 313 is in its axial direction
With two end faces, one of end face is the second end face 3131 of agitating shaft 31, and another end face is the second joint face 3132.
Axially projecting projection 3133, the external diameter and the aperture phase of the first blind hole 3123 of projection 3133 are formed on second joint face 3132
Match somebody with somebody.The second blind hole 3134 extended to inside the second semiaxis 313, the second blind hole 3134 and second are offered on raised 3133 end faces
Semiaxis 313 is coaxially disposed, and the second blind hole 3134 is the second accommodating chamber.
As shown in fig. 7, when the first semiaxis 312 is connected with the second semiaxis 313, the first semiaxis 312 and the second semiaxis 313 are coaxial
Set, the first semiaxis 312 is located at the top of the second semiaxis 313, and the projection 3133 of the second semiaxis 313 is located at the first semiaxis 312
In blind hole 1114 and cooperation is formed, the first joint face 3122 of the first semiaxis 312 contacts with the second semiaxis 313, so that the first half
The first end face 3121 of axle 312 and the phase of second end face 3131 of the second semiaxis 313 deviate from.First semiaxis 312 and the second semiaxis 313
After connection, the outer peripheral wall of the periphery wall of the first semiaxis 312 and the second semiaxis 313 forms a complete cylindrical structure, the first blind hole
3123 connect the closed chamber 311 to be formed inside agitating shaft 31 with the second blind hole 3134, and closed chamber 311 separates with the external world.The first half
Axle 312 is matched somebody with somebody with the second semiaxis 313 by the first blind hole 3123 on the semiaxis 312 of projection 3133 and first on the second semiaxis 313
Close and realize connection, can be by the first semiaxis 312 and the second half when the first semiaxis 312 and the second semiaxis 313 are by opposite pulling force
Axle 313 separates.
In the present embodiment, the second adhesive part 32 is magnet.The shape of second adhesive part 32 is unrestricted, in the present embodiment,
Magnetic part is bar shaped.Second adhesive part 32 is located in the second blind hole 3134 of the second semiaxis 313, and the second adhesive part 32 is pasted onto second
On the bottom wall of blind hole 3134.After second adhesive part 32 and the second semiaxis 313 are fixed, blade 34 is close to first end face 3121, and the
Two adhesive parts 32 are relatively near from first end face 3121 close to second end face 3131, i.e. blade 34, and the second adhesive part 32 is from the second end
Face 3131 is nearer.
Wearing piece 33 is in the second end face 3131 of agitating shaft 31.The material of wearing piece 33 is polytetrafluoroethylene (PTFE), and it has
Have good wearability.Wearing piece 33 is cylindricality pad, and the thickness of wearing piece 33 is far smaller than the length of agitating shaft 31, wearing piece
33 diameter is equal with the diameter of agitating shaft 31.
As shown in figure 1, culture plate 20 is placed in the clamping section formed between four support members 1112 of pedestal 111,
The bottom wall of culture plate 20 contacts with the protuberance 1113 of the inner side of support member 1112.First adhesive part 13 is located under culture plate 20
Side, the axis of the first adhesive part 13 overlap in the center line of culture lattice 21.Culture lattice 21 and a seat in one culture plate 20
The first adhesive part 13 in body 111 corresponds.Culture lattice 21 one stirrer 30 of corresponding placement in culture plate 20, is stirred
The wearing piece 33 in son 30 is mixed to contact with cultivating the bottom wall of lattice 21.The second adhesive part 32 in stirrer 30 will be with the first adhesive part
13 produce magnetic force.
In actual use, the loading culture medium for cultivating optional then property in lattice 21 in culture plate 20, starts motor
Afterwards, all first adhesive parts 13 in pedestal 11 will all rotate, and in the presence of magnetic force, will drive 32 turns of the second adhesive part
It is dynamic, so as to be stirred to the culture medium in culture lattice 21.Because the semiaxis 312 of blade 34 and first in stirrer 30 tilts cloth
Put, by by the lift of the application from culture medium while stirrer 30 is stirred in culture lattice 21 to culture medium, be somebody's turn to do
The direction of lift is opposite with the action direction for the magnetic force that stirrer 30 is subject to.When the rotating speed of stirrer 30 reaches certain, stirring
Lift suffered by son 30 is up to balance with magnetic force, so that whole stirring is suspending in the medium, avoids stirrer 30
Rubbed between culture plate 20, prevent wearer from polluting culture medium, improve mixing quality.In addition, stirred by controlling
The rotating speed of son 30 can also realize moving up and down for stirrer 30, so that stirrer 30 to culture medium uniformly sufficiently stir
Mix.Certainly, when just startup rotating speed is not high, the bottom of stirrer 30 still can rub stirrer 30 with culture plate 20, but
Because the bottom of stirrer 30 is wearing piece 33, wearing piece 33 is not likely to produce abrasion with the friction of culture plate 20.
A kind of bioreactor 100 that the present embodiment provides, using contactless between driver 10 and stirrer 30
Magnetic connects.When needing to observe the culture medium in culture plate 20, directly culture plate 20 can be removed and be observed, nothing
Need to be to stirrer 30 to be taken out from culture plate 20, operation is extremely convenient, and culture medium will not be polluted.This biological respinse
Device 100 can effectively reduce cumbersome assembling during sterilization and close time, only be needed to stirrer in sterilization process
30 individually sterilizings；In addition, also easily stirrer 30 and culture plate 20 can be changed.This bioreactor 100
It can be alternatively arranged as tissue block as the culture instrument of various organs and be digested to unicellular experimental tool of Denging.
In the present embodiment, culture plate 20 is internally formed multiple rectangular array culture lattice 21, can be in limited space simultaneously
Multigroup culture medium is cultivated, is spaced from each other between culture medium, cross pollution will not be caused.Trained in different culture lattice 21
The culture medium of different condition is supported, is advantageous to carry out high flux screening.In addition, the top opening structure of culture plate 20 causes culture medium
Good gas exchanges can be carried out with the external world.
Two pedestals 111 for being used to place culture plate 20 are provided with the present embodiment, in driver 10, i.e., can on driver 10
Two culture plates 20 are laid, improve the service efficiency of whole bioreactor 100.In other specific embodiments, driver
It may also set up one or more pedestals 111 in 10, all first adhesive parts 13 in driver 10 can still be by a motor
Driving.
In the present embodiment, the inside of agitating shaft 31 is provided with closed chamber 311, on the one hand can reduce the density of agitating shaft 31, another
Face can form air column in closed chamber 311, can strengthen the buoyancy of whole stirrer 30, so that stirrer 30 is without at a high speed
Suspended state is just can be under rotation.
In the present embodiment, magnetic part 132 is arranged on the closed chamber 311 of agitating shaft 31, so that magnetic part 132 is not exposed to
In culture medium, avoid polluting culture medium.In addition, after magnetic part 132 is arranged on closed chamber 311, magnetic part 132 will not be by
To the interference of extraneous factor, ensure magnetic part 132 be connected with agitating shaft 31 after fastness.And agitating shaft 31 uses the first semiaxis
312 and second structure that is detachably connected of semiaxis 313, on the one hand facilitate the formation of closed chamber 311, on the other hand facilitate magnetic part
132 installation.
It should be noted that in the present embodiment, the blade 34 in stirrer 30 is three, in other specific embodiments,
Blade 34 in stirrer 30 can be with two or more other numbers of circumference on agitating shaft 31.
In the present embodiment, motor is arranged vertically, and motor is joined directly together with output gear 15.In other specific embodiments,
Motor also can be horizontally disposed, is connected between the output shaft and output gear 15 of motor by the conical gear 141 of a pair of meshing.
Embodiment 2
As shown in figure 11, the present embodiment provides a kind of biologic reaction equipment 200, including controller 210 and above-described embodiment
In bioreactor 100.
Wherein, controller 210 is motor speed controller.Controller 210 electrically connects with the motor in driver 10, control
Device 210 can control the rotating speed of motor, the rotating speed of the first adhesive part 13 and stirrer 30 is controlled so as to realize, for experiment
Repeatability offers precise data guarantee.
The preferred embodiments of the present invention are these are only, are not intended to limit the invention, for those skilled in the art
For member, the present invention can have various modifications and variations.Any modification within the spirit and principles of the invention, being made,
Equivalent substitution, improvement etc., should be included in the scope of the protection.
Claims (10)
1. a kind of bioreactor, it is characterised in that including driver, culture plate and stirrer；
The driver is provided with the first adhesive part, and the driver can drive the first adhesive part to rotate；
The culture plate is positioned on the driver, and the stirrer is positioned in the culture plate；
The stirrer includes the second adhesive part, and the second adhesive part is connected with the first adhesive part magnetic.
2. bioreactor according to claim 1, it is characterised in that the culture plate is internally formed multiple open tops
Culture lattice, the first adhesive part with it is described culture lattice correspond, the stirrer be positioned over it is described culture lattice in.
3. bioreactor according to claim 2, it is characterised in that the culture lattice rectangular array distribution.
4. according to the bioreactor described in claim any one of 1-3, it is characterised in that the driver includes pedestal and moved
Power part, the first adhesive part are rotatably arranged at the pedestal, and the culture plate is positioned on the pedestal, the power
Part is used to drive the first adhesive part to rotate relative to the pedestal.
5. bioreactor according to claim 4, it is characterised in that the pedestal includes at least one pedestal, described
Culture plate is positioned on the pedestal, and the culture plate and the pedestal correspond, and the pedestal is provided with multiple parallel set
The the first adhesive part put, the force piece can drive all first adhesive parts in the pedestal to rotate.
6. bioreactor according to claim 5, it is characterised in that two adjacent described first in the pedestal
Adhesive part is connected by transmission component.
7. bioreactor according to claim 1, it is characterised in that the stirrer also includes agitating shaft and circumference point
At least two blades being distributed on the outside of the agitating shaft, the second adhesive part are fixed on the agitating shaft；
Plane where the blade is reference planes, and the axis of the agitating shaft is axis of reference, the reference planes and institute
State axis of reference to set in angle, the reference planes and the reference axis are in that angle is acute angle.
8. bioreactor according to claim 7, it is characterised in that the inside of the agitating shaft is provided with closed chamber, institute
The second adhesive part is stated in the closed chamber.
9. the bioreactor according to claim 7 or 8, it is characterised in that the stirrer also includes wearing piece, described
Wearing piece is fixed on the bottom of the agitating shaft.
10. a kind of biologic reaction equipment, it is characterised in that anti-including the biology described in controller and claim any one of 1-9
Device is answered, the controller is used to control the driver.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710855964.8A CN107435023A (en) | 2017-09-19 | 2017-09-19 | A kind of bioreactor and biologic reaction equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710855964.8A CN107435023A (en) | 2017-09-19 | 2017-09-19 | A kind of bioreactor and biologic reaction equipment |
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| CN107435023A true CN107435023A (en) | 2017-12-05 |
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|---|---|---|---|
| CN201710855964.8A Pending CN107435023A (en) | 2017-09-19 | 2017-09-19 | A kind of bioreactor and biologic reaction equipment |
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| CN111961573A (en) * | 2020-08-04 | 2020-11-20 | 北京君立康生物科技有限公司 | Culture medium preparation equipment |
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