CN201280562Y - Suspending cell culture bottle with oar - Google Patents
Suspending cell culture bottle with oar Download PDFInfo
- Publication number
- CN201280562Y CN201280562Y CNU2008201105585U CN200820110558U CN201280562Y CN 201280562 Y CN201280562 Y CN 201280562Y CN U2008201105585 U CNU2008201105585 U CN U2008201105585U CN 200820110558 U CN200820110558 U CN 200820110558U CN 201280562 Y CN201280562 Y CN 201280562Y
- Authority
- CN
- China
- Prior art keywords
- bottle
- cell culture
- fixed link
- suspension cell
- culture bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 14
- 238000003756 stirring Methods 0.000 claims description 16
- 239000000725 suspension Substances 0.000 claims description 12
- 229920003023 plastic Polymers 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 6
- 238000005255 carburizing Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 229920002529 medical grade silicone Polymers 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 8
- 238000012423 maintenance Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000010276 construction Methods 0.000 abstract 1
- 238000009434 installation Methods 0.000 abstract 1
- 238000003760 magnetic stirring Methods 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 238000010008 shearing Methods 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 2
- 238000004114 suspension culture Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A suspended cell culture bottle with a paddle comprises a bottle body, a bottle cover and an agitating device, wherein, the upper end of the agitating device is connected with the inner side of the bottle cover; and the lower end of the agitating device extends into the bottle body and is close to the bottom end of the bottle body. The suspended cell culture bottle is characterized in that the agitating device comprises a fixed link, a connecting hose, a shaft lever, a connecting rod, a magnetic rod and a paddle, wherein, the upper end of the fixed link is fixedly connected with the center of the inner side of the bottle cover; the lower end of the fixed link is connected with the upper end of the shaft lever through the connecting hose; the upper end of the shaft lever is coaxially and rotationally connected with the upper end of the connecting rod; the lower end of the connecting rod is vertically connected with the middle of an agitating paddle; the magnetic rod is vertically connected with the middle of the agitating paddle; and the magnetic rod and the agitating paddle are in a cruciform construction. The utility model has the advantages of simple structure, convenient disassembly and installation of a plurality of connections, easy maintenance, low production cost, good agitating effect and easy popularization and application.
Description
Technical field
The utility model relates to a kind of band oar suspension cell culture bottle, be mainly used in biological technical field, as bacterium, fungi, the isocellular suspension culture of plant, especially be fit to suspension domestication and suspension culture to the adherent mammalian cell and the insect cell of shearing force sensitivity.
Background technology
The primary structure of prior art comprises bottle, bottle cap and whipping appts, whipping appts is made up of fixed link and revolving stirrer, fixedly connected with the bottle cap inboard in the upper end of fixed link, the lower end of fixed link and rigid revolving stirrer are rotationally connected, and blade that revolving stirrer connects is a vertical vane.The shortcoming of its existence is: fixed link and bottle cap be decentraction often, causes revolving stirrer to produce eccentric vibration when rotating, and increases rotational resistance, influences mixing effect; The rigid stirring of revolving stirrer intelligence can not be regulated the diameter of stirring, and adaptability is relatively poor, can not satisfy various demand; The vertical agitating vane pair cell shearing force that revolving stirrer connected is bigger, easily killer cell.
Summary of the invention
The purpose of this utility model just provides a kind of band oar suspension cell culture bottle, to solve often decentraction of fixed link that prior art exists and bottle cap, causes revolving stirrer to produce eccentric vibration when rotating, and the increase rotational resistance influences mixing effect; The vertical agitating vane pair cell shearing force that revolving stirrer connected is bigger, easily the problem of killer cell.
The technical solution of the utility model is: comprise bottle, bottle cap and whipping appts, the upper end of whipping appts is connected with bottle cap is inboard, the lower end of whipping appts is stretched in the bottle and near the bottle bottom, it is characterized in that: described whipping appts comprises fixed link, connect flexible pipe, axostylus axostyle, union lever, bar magnet and blade, the upper end of fixed link is connected with the inboard center fixation of described bottle cap, the lower end of fixed link is connected with the upper end of axostylus axostyle by connecting flexible pipe, the lower end of this axostylus axostyle is connected with the upper end coaxial rotation of union lever, lower end at this union lever links to each other with the intermediate vertical of a stirring rake, connect a bar magnet in this stirring rake intermediate vertical, this bar magnet and described stirring rake are cruciform.
By the sleeve nut that is threaded, the outward flange of the upper end of described union lever is socketed in the reducing hole of this sleeve nut in the lower end of described axostylus axostyle.
Described bottle is the standard blue lid reagent bottle of bottleneck diameter 45mm or the bore bottle greater than 45mm, adopts the upper end that the single port bottle of opening is arranged or also is provided with the bottle of opening in the two side arms of upper end open.
Described fixed link and bottle cap are structure as a whole or are fastening mutually by the stainless steel nut.
The lower end of fixed link is inserted in the upper end of described connection flexible pipe.
Place, close two ends at described connection flexible pipe respectively is provided with through hole.
Described connection flexible pipe adopts medical silicone tube, and described fixed link and union lever adopt CARBURIZING FURNACE FOR STAINLESS FASTENER or plastics, and described bottle cap, axostylus axostyle and rotary sleeve are made of plastic.
Described stirring rake is made of a pair of blade and intermediate ring, and the periphery relative at intermediate ring respectively radially connects a described blade, and the pitch angle of this blade is between 0 to 90 degree; Described bar magnet inserts in this intermediate ring, and the lower end of described union lever radially is connected the mid-way that this intermediate ring periphery is connected with described two blades.
This device places on the magnetic stirring apparatus in use, and the rotatingfield that is produced by magnetic stirring apparatus drives the bar magnet rotation.Owing to connect the effect of flexible pipe, make to stir between bar magnet and magnetic stirring apparatus magnetic pole to reach best linkage status automatically; Hose connection adjustable the whole series are gone into the degree of depth, but the total length of the whole whipping appts of regulated at will is suitable for different demands.By changing this device agitating vane angle of inclination, can between enhancing mixing effect and reduction shearing force, reach good balance.This apparatus structure is simple, and many places are connected to and can for convenience detachly install, maintenance easily, and production cost is low, is easy to apply.
Description of drawings
Fig. 1 is the overall structure synoptic diagram of an embodiment of the utility model;
Fig. 2 is that the B of whipping appts among Fig. 1 is to view;
Fig. 3 is that the A of whipping appts among Fig. 1 is to view.
Embodiment
Referring to Fig. 1～Fig. 3, the utility model comprises bottle 1, bottle cap 3 and whipping appts.Described whipping appts comprises fixed link 4, connects flexible pipe 5, axostylus axostyle 7, union lever 10, bar magnet 11 and blade 12.The upper end of fixed link 4 is connected with described bottle cap 3 inboard center fixation, the lower end of fixed link 4 is by connecting the upper end socket of flexible pipe 5 and axostylus axostyle 7, the lower end of this axostylus axostyle 7 is by the sleeve nut 9 that is threaded, the outward flange 8 of the upper end of described union lever 10 is socketed in the reducing hole of this sleeve nut 9, and both can relatively rotate.Lower end at this union lever 10 links to each other with the intermediate vertical of a stirring rake, connects a bar magnet 11 in this stirring rake intermediate vertical, and this bar magnet 11 is cruciform (as shown in Figure 3) with described stirring rake.
Described stirring rake is made of a pair of blade 12 and intermediate ring 13, respectively radially connects a described blade 12 at intermediate ring 13 relative peripherys, and the pitch angle of this blade 12 is between 0 to 90 degree.Described bar magnet 11 inserts in these intermediate rings 13, and the lower end of described union lever 10 radially is connected the mid-way (being the top of intermediate ring 13 shown in Figure 3) that these intermediate ring 13 peripherys are connected with described two blades.
The standard blue lid reagent bottle (or greater than this bore) that described bottle 1 is bottleneck diameter 45mm, there is the single port bottle of opening the employing upper end or also is provided with the bottle of opening in the two side arms of upper end open.The volume of bottle 1 is 100ml, 250ml, 500ml, 1000ml, 20000ml or bigger.
Described fixed link 4 is structure as a whole with bottle cap 3 or is fastening mutually by the stainless steel nut.
Utilize the softness characteristics that connects flexible pipe 5, the decentraction that can overcome owing to fixed link 4 and bottle cap 3 causes whipping appts to produce the shortcoming that vibration influences mixing effect when rotated.The degree of depth that the lower end of fixed link is inserted in the upper end of described connection flexible pipe 5 can be regulated.
Raise to cause to connect and come off for fear of connecting flexible pipe 5 internal gas Yin Wendu, can respectively be provided with through hole 6, connect flexible pipe 5 interior gas or liquid to discharge at the place, close two ends of described connection flexible pipe 5.
Described connection flexible pipe 5 adopts medical silicone tube, and described fixed link 4 and union lever 10 adopt CARBURIZING FURNACE FOR STAINLESS FASTENER or plastics, and described bottle cap 3, axostylus axostyle 7 and sleeve nut 9 are made of plastic.
A whole set of the utility model device can carry out 121 ℃ of autoclavings before use to be handled.
This device is driven by slow-revving magnetic stirring apparatus in use, is about to this device and is placed on the platform of magnetic stirring apparatus, and the rotatingfield that magnetic stirring apparatus produces attracts to drive bar magnet 11 and blade 12 rotations, thereby reaches the purpose of stirring.Two blades 12 tilt (similar fan blade, the angle of inclination is between 0 to 90 degree) to a direction, make bottle interior liquid produce swirling flow longitudinally, and mixing effect is better.
The utility model also can be by the length of adjusting bar magnet 11 and big or small length, the angle of inclination of blade, to reach best mixing effect when using.
Claims (8)
1, a kind of band oar suspension cell culture bottle, comprise bottle, bottle cap and whipping appts, the upper end of whipping appts is connected with bottle cap is inboard, the lower end of whipping appts is stretched in the bottle and near the bottle bottom, it is characterized in that: described whipping appts comprises fixed link, connect flexible pipe, axostylus axostyle, union lever, bar magnet and blade, the upper end of fixed link is connected with the inboard center fixation of described bottle cap, the lower end of fixed link is connected with the upper end of axostylus axostyle by connecting flexible pipe, the lower end of this axostylus axostyle is connected with the upper end coaxial rotation of union lever, lower end at this union lever links to each other with the intermediate vertical of a stirring rake, connect a bar magnet in this stirring rake intermediate vertical, this bar magnet and described stirring rake are cruciform.
2, band oar suspension cell culture bottle according to claim 1 is characterized in that: by the sleeve nut that is threaded, the outward flange of the upper end of described union lever is socketed in the reducing hole of this sleeve nut in the lower end of described axostylus axostyle.
3, band oar suspension cell culture bottle according to claim 1, it is characterized in that: described bottle is the standard blue lid reagent bottle of bottleneck diameter 45mm or the bore bottle greater than 45mm, adopts the upper end that the single port bottle of opening is arranged or also is provided with the bottle of opening in the two side arms of upper end open.
4, band oar suspension cell culture bottle according to claim 1 is characterized in that: described fixed link and bottle cap are structure as a whole or are fastening mutually by the stainless steel nut.
5, band oar suspension cell culture bottle according to claim 1, it is characterized in that: the lower end of fixed link is inserted in the upper end of described connection flexible pipe.
6, band oar suspension cell culture bottle according to claim 1 is characterized in that: the place, close two ends at described connection flexible pipe respectively is provided with through hole.
7, band oar suspension cell culture bottle according to claim 1 is characterized in that: described connection flexible pipe adopts medical silicone tube, and described fixed link and union lever adopt CARBURIZING FURNACE FOR STAINLESS FASTENER or plastics, and described bottle cap, axostylus axostyle and rotary sleeve are made of plastic.
8. band oar suspension cell culture bottle according to claim 1, it is characterized in that: described stirring rake is made of a pair of blade and intermediate ring, and the periphery relative at intermediate ring respectively radially connects a described blade, and the pitch angle of this blade is between 0 to 90 degree; Described bar magnet inserts in this intermediate ring, and the lower end of described union lever radially is connected the mid-way that this intermediate ring periphery is connected with described two blades.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201105585U CN201280562Y (en) | 2008-06-05 | 2008-06-05 | Suspending cell culture bottle with oar |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201105585U CN201280562Y (en) | 2008-06-05 | 2008-06-05 | Suspending cell culture bottle with oar |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201280562Y true CN201280562Y (en) | 2009-07-29 |
Family
ID=40927442
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008201105585U Expired - Lifetime CN201280562Y (en) | 2008-06-05 | 2008-06-05 | Suspending cell culture bottle with oar |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201280562Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031824A (en) * | 2013-03-05 | 2014-09-10 | 上海山富科学仪器有限公司 | Stirrer |
| CN107435023A (en) * | 2017-09-19 | 2017-12-05 | 中国医学科学院输血研究所 | A kind of bioreactor and biologic reaction equipment |
| CN107913631A (en) * | 2017-11-27 | 2018-04-17 | 深圳康泰生物制品股份有限公司 | A kind of flexible axle magnetic agitation bottle |
-
2008
- 2008-06-05 CN CNU2008201105585U patent/CN201280562Y/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031824A (en) * | 2013-03-05 | 2014-09-10 | 上海山富科学仪器有限公司 | Stirrer |
| CN107435023A (en) * | 2017-09-19 | 2017-12-05 | 中国医学科学院输血研究所 | A kind of bioreactor and biologic reaction equipment |
| CN107913631A (en) * | 2017-11-27 | 2018-04-17 | 深圳康泰生物制品股份有限公司 | A kind of flexible axle magnetic agitation bottle |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: INNER MONGOLIA BIGVET BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: LI SHAOYING Effective date: 20110331 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 132109 DEPARTMENT OF PREVENTIVE VETERINARY MEDICINE, INSTITUTE OF SPECIAL WILD ECONOMIC ANIMAL AND PLANT SCIENCE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES, ZUOJIA TOWN, CHANGYI DISTRICT, JILIN CITY, JILIN PROVINCE TO: 010176 200 METERS EAST OF POLICE STATION, SHENGLE ECONOMIC DEVELOPMENT ZONE, HELIN COUNTY, HOHHOT CITY, INNER MONGOLIA AUTONOMOUS REGION |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20110331 Address after: 010176 the Inner Mongolia Autonomous Region and Hohhot County police station Shengle Economic Development Zone East 200 meters Patentee after: INNER MONGOLIA BIWEI ANTAI BIOTECHNOLOGY CO., LTD. Address before: 132109 Jilin District, Jilin City, Changyi District, left town, Chinese Academy of Agricultural Sciences Specialty Research Institute, preventive veterinary medicine room Patentee before: Li Shaoying |
|
| CX01 | Expiry of patent term |
Granted publication date: 20090729 |
|
| CX01 | Expiry of patent term |