CN111961573B - Culture medium preparation equipment - Google Patents

Culture medium preparation equipment Download PDF

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Publication number
CN111961573B
CN111961573B CN202010773695.2A CN202010773695A CN111961573B CN 111961573 B CN111961573 B CN 111961573B CN 202010773695 A CN202010773695 A CN 202010773695A CN 111961573 B CN111961573 B CN 111961573B
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magnetic
culture medium
piece
magnetic part
preparation chamber
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CN111961573A (en
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刘科
厉刚
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Beijing Junlikang Biotechnology Co ltd
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Beijing Junlikang Biotechnology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M99/00Subject matter not otherwise provided for in other groups of this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of culture medium preparation, and provides culture medium preparation equipment. The culture medium preparation equipment comprises: a preparation chamber; a support member located in the preparation chamber, movable in the preparation chamber, and configured to place a containing body containing a culture medium; the first magnetic piece is positioned in the preparation chamber and fixed at the bottom of the support piece; the second magnetic part is positioned outside the preparation chamber and is correspondingly arranged below the first magnetic part; and the first driving part is connected with the second magnetic part and drives the second magnetic part to move. According to the culture medium preparation device, the first magnetic part and the second magnetic part are respectively positioned inside and outside the preparation chamber, and the first magnetic part and the second magnetic part are acted by a magnetic field. Specifically, when the second magnetic part moves, the first magnetic part moves under the action of a magnetic field. The first magnetic part moves to drive the support part to move, so that the culture medium in the accommodating body on the support part is shaken to achieve the purpose of full mixing.

Description

Culture medium preparation equipment
Technical Field
The invention relates to the technical field of culture medium preparation, in particular to culture medium preparation equipment.
Background
The culture medium is artificially prepared and is suitable for the growth, separation and identification of microorganisms. The basic culture medium generally contains basic nutrients such as peptone, beef extract powder, yeast extract powder, sodium chloride and agar. In the culture medium used for clinical and food hygiene test, it is often necessary to add bacteriostatic agents, indicators, blood, saccharides and other components to facilitate the separation and identification of specific bacteria, while the culture medium used in virus culture and vaccine production needs to add different kinds of amino acids, nucleotides, inorganic salts, growth factors and other substances.
The culture medium as basic material for microbe research is used in the following state: first, a completely sterile state; secondly, all components in the culture medium are balanced and effective, and the components of the culture medium are not lost or denatured in the preparation process; finally, the preparation time is short to ensure the activity of the microorganisms in the sample to be tested during inoculation, especially the clinical samples and anaerobic bacteria.
In order to make the culture medium reach the state when in use, the culture medium is generally prepared and used in a laboratory at present, and the components of different culture media need to be ensured to be fully mixed in the preparation process. Mechanical stirring is often required during the mixing of the different media components. This kind of stirring mode need with the stirring part directly stretch into the container that has different culture medium components in the middle of, can't guarantee that the culture medium component is in aseptic condition to lead to culture medium component loss and degeneration easily.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art or the related art.
One of the objects of the invention is: the utility model provides a culture medium preparation equipment, solve that exist among the prior art can't guarantee that the culture medium component is in sterile condition to lead to the problem of culture medium component loss and degeneration easily.
To achieve the object, the present invention provides a medium formulating device comprising:
a preparation chamber;
a support member located in the preparation chamber, movable in the preparation chamber, and configured to place a containing body containing a culture medium;
the first magnetic piece is positioned in the preparation chamber and fixed at the bottom of the support piece;
the second magnetic part is positioned outside the preparation chamber and is correspondingly arranged below the first magnetic part;
and the first driving part is connected with the second magnetic part and drives the second magnetic part to move.
According to one embodiment of the present invention, the medium formulating device further comprises:
the mounting seat is mounted on the bottom plate of the preparation chamber, is positioned below the supporting piece and is connected with the supporting piece through at least three eccentric wheels;
at least one of the eccentric wheels is a driving wheel, the driving wheel is connected with a first end of a rotating shaft, and a second end of the rotating shaft penetrates through the mounting seat and then is connected with the first magnetic part;
the first driving part is a rotary driving part, the rotary driving part drives the second magnetic part to rotate, and the second magnetic part drives the first magnetic part to rotate.
According to one embodiment of the present invention, the mounting base is connected to the bottom plate through a turning shaft, a first limiting member is disposed on the mounting base, a second limiting member is disposed in the preparation chamber, and the first limiting member and the second limiting member are configured to: the first limiting piece and the second limiting piece are in limiting fit, and the mounting base is locked on the bottom plate; the first limiting part and the second limiting part are relieved from limiting, and the mounting base overturns around the overturning shaft under the action of external force.
According to one embodiment of the present invention, the mounting base is a supporting plate, the first limiting member is a plug fixed at the bottom of the supporting plate, the second limiting member is a socket, and the socket is connected to the second driving member and driven by the second driving member to leave or sleeve the plug.
According to one embodiment of the invention, the first driving part is mounted on a guide rod, and the first driving part is connected with an electric push rod and moves along the axial direction of the guide rod under the action of the electric push rod.
According to one embodiment of the present invention, the first driving member is a driving motor, the driving motor is connected to the magnet holder through a speed reduction member, the first magnetic member is a magnetic conductive plate, and the second magnetic member is a magnet fixed to the magnet holder.
According to one of the embodiments of the present invention, the support member is a net bracket.
According to one embodiment of the invention, the preparation chamber is a temperature-controlled chamber.
According to one embodiment of the present invention, the medium formulating device further comprises:
a mounting member, located outside the preparation chamber, configured to place a container containing a culture medium;
the containing body comprises a first containing body for containing a coagulant solution and a second containing body for containing a nutrient solution and/or an additive solution;
the supporting piece is used for placing the first package body, and the mounting component is used for placing the second package body.
According to one embodiment of the present invention, the medium formulating device further comprises:
and the first communication pipe is used for introducing the nutrient solution and/or the additive in the second packaging body into the first packaging body.
The technical scheme of the invention has the following advantages: according to the culture medium preparation device, the first magnetic part and the second magnetic part are respectively positioned inside and outside the preparation chamber, and the first magnetic part and the second magnetic part are acted by a magnetic field. Specifically, when the second magnetic part moves, the first magnetic part moves under the action of a magnetic field. The first magnetic part moves to drive the support part to move, so that the culture medium in the accommodating body on the support part is shaken to achieve the purpose of full mixing.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of a part of a medium preparing apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of a part of a medium dispensing apparatus in an embodiment of the present invention from another perspective;
FIG. 3 is a schematic view of a connection relationship between the driving wheel and the first magnetic member according to an embodiment of the present invention;
FIG. 4 is a schematic structural view of an assembly with a support and a mount shown in an embodiment of the invention;
fig. 5 is a schematic view showing a connection relationship among the first driving part, the speed reducing part, the magnet holder and the second magnetic member in the embodiment of the present invention;
FIG. 6 is a schematic diagram of a media preparation apparatus according to an embodiment of the present invention, with parts of the media preparation apparatus removed and the power rod removed;
FIG. 7 is a schematic view of a medium preparing apparatus according to an embodiment of the present invention, partially removed from the electric push rod, from another perspective;
in the figure: 1. a second magnetic member; 2. a speed reduction part; 3. a first drive member; 4. a magnet holder; 5. an electric push rod; 6. a guide bar; 7. a first magnetic member; 8. an eccentric wheel; 9. a plug; 10. a rotating shaft; 11. a turning shaft; 12. a mounting base; 13. a support member; 14. a socket is provided.
Detailed Description
Embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example" or "some examples" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this description, a schematic representation of the above terms does not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Moreover, various embodiments or examples and features of various embodiments or examples described in this specification can be combined and combined by one skilled in the art without being mutually inconsistent.
In the description of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
According to the embodiment of the invention, the culture medium preparation equipment is provided, and the problems that the components of the culture medium can not be ensured to be in a sterile state and the loss and denaturation of the components of the culture medium are easily caused in the prior art are solved.
Referring to fig. 1 and 2, the medium preparing apparatus includes a preparing chamber (not shown), a supporting member 13, a first magnetic member 7, a second magnetic member 1, and a first driving part 3. Wherein the support 13 is located in the preparation chamber, e.g. at the bottom of the preparation chamber, movable therein, and the support 13 is configured to place a receptacle containing the culture medium. The first magnetic part 7 is positioned in the preparation chamber and fixed at the bottom of the support part 13; the second magnetic part 1 is positioned outside the preparation chamber and is correspondingly arranged below the first magnetic part 7; the first driving part 3 is connected with the second magnetic part 1 and drives the second magnetic part 1 to move; the second magnetic part 1 moves to drive the first magnetic part 7 to move.
In the culture medium preparation device, the first magnetic part 7 and the second magnetic part 1 are respectively positioned inside and outside the preparation chamber, and the first magnetic part and the second magnetic part act through a magnetic field. Specifically, when the second magnetic member 1 moves, the first magnetic member 7 is moved by the action of the magnetic field. The first magnetic part 7 moves to drive the support part 13 to move, so that the culture medium in the accommodating body on the support part 13 is shaken to achieve the purpose of full mixing.
Moreover, the first magnetic part 7 and the second magnetic part 1 are respectively positioned inside and outside the preparation chamber and interact with each other through a magnetic field without direct contact, so that the problem of tightness caused by the transmission part passing through the preparation chamber can be avoided.
The second magnetic member 1 is located outside the preparation chamber and is correspondingly disposed below the first magnetic member 7, and the "corresponding disposition" refers to the following conditions: the second magnetic member 1 is in the magnetic field of the first magnetic member 7, or the first magnetic member 7 is in the magnetic field of the second magnetic member 1, so that the first magnetic member 7 moves along with the second magnetic member 1 when the second magnetic member 1 moves.
The first magnetic member 7 refers to an object that reacts in some way to a magnetic field and does not require itself to be able to generate a magnetic field. The first magnetic member 7 can be made of any magnetic material, such as magnet, low-carbon steel, etc.; of course, the first magnetic member 7 may be an energized coil. Similarly, the second magnetic member 1 may also be made of any magnetic material or be in the form of an electric coil, and the specific form is not limited as long as the first magnetic member 7 can drive the second magnetic member 1 to move through a magnetic field.
Note that the movement modes of the first magnetic member 7 and the second magnetic member 1 are not limited. For example, the first driving part 3 can drive the second magnetic part 1 to rotate, and the second magnetic part 1 drives the first magnetic part 7 to rotate. For another example, the first driving part 3 can drive the second magnetic member 1 to move linearly, and the second magnetic member 1 drives the first magnetic member 7 to move linearly.
In the case of "the first driving part 3 drives the second magnetic part 1 to rotate, and the second magnetic part 1 drives the first magnetic part 7 to rotate": the medium formulating device further comprises a mounting 12, the mounting 12 being mounted to the floor of the formulating chamber such that the mounting 12 is located below the support 13.
Wherein the mounting 12 can be connected to the support 13 by at least three eccentrics 8; one eccentric wheel 8 is connected with a first end of a rotating shaft 10, and a second end of the rotating shaft 10 penetrates through a mounting seat 12 and then is connected with a first magnetic piece 7; the first driving component 3 is a rotation driving component, the rotation driving component drives the second magnetic component 1 to rotate, and the second magnetic component 1 drives the first magnetic component 7 to rotate.
In the case of three eccentrics 8, a secure mounting of the support 13 on the mounting 12 is ensured. Of course, the number of the eccentric wheels 8 is not limited by the examples, for example, the eccentric wheels 8 may be four, five, etc.
The eccentric wheel 8 is divided into a driving wheel and a driven wheel, and the driving wheel and the driven wheel are fixedly connected with the supporting piece 13. For the driving wheel, it is connected to the first end of the rotating shaft 10, and the second end of the rotating shaft 10 is connected to the first magnetic member 7 after passing through the mounting seat 12. For the driven wheel, the driven wheel is connected with a first end of a mounting shaft, a second end of the mounting shaft is fixed on the mounting base 12, and the driven wheel can rotate relative to the mounting shaft. Furthermore, when the first magnetic member 7 rotates, the rotating shaft 10 of the driving wheel drives the driving wheel to rotate, the driving wheel drives the supporting member 13 to rotate, and the supporting member 13 drives the driven wheel to rotate relative to the mounting shaft when rotating. When the supporting member 13 moves circularly in the plane, the medium components in the accommodating body form a vortex, so that different medium components are fully mixed.
Wherein the gap between the driving wheel and the first magnetic element 7 is matched with the thickness of the mounting seat 12.
It should be noted that, of course, it is not necessary to provide the eccentric 8 between the supporting member 13 and the mounting base 12 in order to allow the supporting member 13 to rotate relative to the mounting base 12. For example, a connecting rod may be further disposed between the supporting member 13 and the mounting seat 12, the first magnetic member 7 is fixedly connected to one end of the connecting rod, and the other end of the connecting rod is connected to the supporting member 13, so that the first magnetic member 7 rotates to drive the connecting rod to rotate, and finally, the supporting member 13 is driven to move along a circle. For another example, a cam may be provided between the supporting member 13 and the mounting seat 12, and the supporting member 13 may be driven to rotate by the cam.
It should also be mentioned that the mounting 12 is not necessarily provided, for example, in the case of the above-mentioned eccentric 8, the second end of the mounting shaft of the driven wheel can be directly fixed to the bottom plate of the preparation chamber, while the rotational axis 10 of the driving wheel is directly provided at the bottom of the support 13.
When the second magnetic member 1 rotates, a rotating magnetic field is generated, and the rotating magnetic field generates closed magnetic lines of force through the first magnetic member 7, so that the magnetic force reaches the maximum, and the first magnetic member 7 rotates along with the magnetic field. The rotation of the first magnetic element 7 causes the support element 13 to move along a circle in a plane, so that the medium in the container forms a vortex, and the mixing of different medium components is accelerated.
Under the condition that the first driving part 3 drives the second magnetic part 1 to do linear motion and the second magnetic part 1 drives the first magnetic part 7 to do linear motion: a linear guide rail is arranged on the bottom plate of the preparation chamber, and the supporting piece 13 is arranged on the linear guide rail through a sliding block and can move linearly along the linear guide rail. The sliding block and the first magnetic part 7 are fixed, and then when the second magnetic part 1 moves linearly, the second magnetic part 1 drives the first magnetic part 7 to move linearly, and the first magnetic part 7 drives the sliding block to move linearly.
Also, in order to allow the support member 13 to reciprocate in a straight line in the preparation chamber, the connection relationship between the support member 13 and the preparation chamber is not limited to the connection form of the guide rail and the slider, but may also take any construction form that has been disclosed in the prior art. For example, a rack and pinion connection between the support member 13 and the dosing chamber may also be used, in which case the rack is fixed to the bottom plate of the dosing chamber, the pinion is fixed to the first magnetic member 7, and the pinion is fixed to the support member 13.
Of course, the mounting base 12 may be provided in the preparation chamber, and the linear guide or the rack described above may be fixed to the mounting base 12.
It should be noted that, when the second magnetic member 1 drives the first magnetic member 7 to move linearly, the shaking effect of the first magnetic member is less than that of the second magnetic member 1, and the mixing effect of the culture medium is less than that of the second magnetic member 7.
Referring to fig. 4, when the mounting base 12 is disposed, the mounting base 12 is connected to a bottom plate (not shown in the figure) of the preparation chamber through the turning shaft 11, the mounting base 12 is provided with a first limiting member, the preparation chamber is provided with a second limiting member, and the first limiting member and the second limiting member are configured as follows: the first limiting piece and the second limiting piece are in limiting fit, and the mounting base 12 is locked on the bottom plate; the first limiting piece and the second limiting piece are relieved from limiting, and the mounting base 12 is forced to turn around the turning shaft 11.
In the process of mixing different culture medium components, the first limiting part and the second limiting part form limiting fit, the mounting base 12 is locked on the bottom plate, the mounting base 12 is basically kept horizontal at the moment, and then the supporting part 13 on the mounting base 12 is kept in a horizontal state. After the mixing process is completed, in order to facilitate discharging the mixed culture medium from the preparation chamber, the first limiting member and the second limiting member are released, the mounting base 12 is turned around the turning shaft 11 under the action of external force, and the support member 13 and the accommodating body are tilted, so that the culture medium is conveniently discharged. The external force applied to the mounting base 12 generally refers to the weight of the containing body and the culture medium therein.
The form of the mutual rotatable connection between the turning shaft 11 and the base plate can be regarded as a two-point connection. When the first limiting member and the second limiting member are in limiting fit, it can be seen that a three-point connection is formed between the mounting base 12 and the preparation chamber, so that the mounting base 12 is fixed on the bottom plate of the preparation chamber.
In particular, when the vessel is tilted, the gas in the medium does not escape with the solution, and a bubble-free medium can be obtained.
When the mounting base 12 is mounted in the preparation chamber, the first stopper is mounted at the rear side of the mounting base 12, that is, at the side far away from the door body of the preparation chamber, and the turning shaft 11 is disposed along the width direction of the preparation chamber. Therefore, when the first limiting part and the second limiting part are relieved from limiting, the accommodating body and the culture medium in the accommodating body, which are arranged close to the preparation room door body, can generate the action of gravity on the front side of the support part 13, so that the front side of the support part 13 sinks and the back side of the support part tilts upwards.
Referring to fig. 4, the mounting base 12 is a supporting plate. The surface area of the mounting seat 12 can be set smaller than that of the supporting member 13, specifically, as long as the mounting requirement of the supporting member 13 is met, thereby reducing the weight and manufacturing cost of the mounting seat 12.
According to the embodiment of the invention, the first limiting member may be a plug 9 fixed at the bottom of the supporting plate, and the corresponding second limiting member may be a socket 14, and the socket 14 is connected to the second driving member and driven by the second driving member to move away from or to sleeve the plug 9.
Of course, the specific form of the first limiting member and the second limiting member is not limited herein, and any form of structure that can achieve limiting matching may be adopted. For example, the first limiting part and the second limiting part may also adopt mutually matched limiting pressing plates; for another example, a pin and a groove that mate with each other may also be used.
The second driving part can adopt a motor, a hydraulic cylinder, an air cylinder and the like.
Referring to fig. 1 and 2 again, the first driving member 3 is mounted on the guide rod 6, and the first driving member 3 is connected to the electric push rod 5 and moves along the axial direction of the guide rod 6 under the action of the electric push rod 5. Furthermore, after the culture medium mixing process is completed, the electric push rod 5 extends to drive the first driving part 3 and the second magnetic part 1 to be away from the first magnetic part 7, so that the acting force between the first magnetic part 7 and the second magnetic part 1 is minimized. Wherein, when the acting force between the first magnetic part 7 and the second magnetic part 1 is reduced, the overturning of the mounting seat 12 can be facilitated.
Of course, it should be noted that the electric push rod 5 may not be provided, and at this time, only the position of the turning shaft 11 needs to be reasonably designed, please refer to fig. 6 and 7, so that the weight of the front part close to the turning shaft 11 is greater than the weight of the rear part, and when the first limiting member and the second limiting member are unlocked, the magnetic attraction between the first magnetic member 7 and the second magnetic member 1 can be overcome, so as to realize the turning and tilting of the mounting base 12.
According to an embodiment of the present invention, referring to fig. 5, the first driving member 3 is a driving motor, the driving motor is connected to the magnet base 4 through the speed reducing member 2, the second magnetic member 1 is a magnet fixed to the magnet base 4, and the magnet includes magnet blocks respectively disposed at two sides of the magnet base 4; the first magnetic member 7 is a magnetic conductive plate. In this case, the output shaft of the first driving member 3 rotates, and the rotational motion is transmitted to the magnet holder 4 through the speed reduction member 2, and the magnet holder 4 rotates the second magnetic member 1.
Of course, the specific manner of the first driving part 3 is not limited by the examples herein, and it can adopt any form disclosed in the prior art, and as long as it can drive the first magnetic part 7 to move.
In fig. 4, the supporting member 13 is a net bracket. By using the net-like bracket, not only can the weight be reduced and the driving of the supporting member 13 be ensured, but also the accommodating body can be more sufficiently exposed. Of course, the specific structural form of the supporting member 13 is not limited as long as the supporting requirement of the accommodating body is satisfied.
According to the embodiment of the invention, the preparation chamber is a temperature control chamber, and the temperature of the culture medium is adjusted by adjusting the temperature of the temperature control chamber. It should be noted that when the supporting member 13 is a net-shaped bracket, it can ensure the accommodating body to be in more sufficient contact with air, and further ensure that the accommodating body has better temperature adjusting effect.
Since the components of the culture medium can be classified into a coagulant, a nutrient and an additive, any one of a coagulant solution, a nutrient solution and an additive solution can be contained in the container. Wherein the coagulant solution is obtained by mixing coagulant and water according to a set proportion. Similarly, the nutrient solution is obtained by mixing a nutrient and water according to a set proportion; the additive solution is obtained by mixing an additive and water according to a set proportion.
Under the premise, the corresponding culture medium can be prepared by only mixing at least two of the coagulant solution, the nutrient solution and the additive solution.
In one embodiment, the main component of the coagulant solution is agar, in which case the coagulant solution is also agar gel. The melting point of the agar gel is about 90-95 ℃, and the freezing point is 40-45 ℃, which determines that the agar can be used as a coagulant of a microorganism culture medium to prepare the culture medium into a required shape at a lower temperature.
In one embodiment, the medium formulating device further comprises a mounting member. The mounting member is located outside the preparation chamber and is configured to receive a container containing a culture medium. The receiving body may be in the form of a package. The components of the medium are encapsulated by the packaging. Different media components are enclosed in different packages, and the different packages enclosing the different media components are placed on the mounting part and the support 13, respectively.
In order to distinguish the packages inside and outside the preparation chamber, the package placed on the support 13 is named a first package, and the package placed on the mounting member is named a second package. The first packaging body is used for containing coagulant solution; the second package is used for containing nutrient solution and/or additive solution.
Wherein the first package and the second package are preferably, but not necessarily, in the form of packages. Sterile bags can be selected for the packaging bag, so that the components of the culture medium can be stored in a sterile environment. Moreover, the packaging bag is preferably selected from soft packaging bags, so that the packaging bag is convenient to transport and store.
Since most of the culture medium requires the coagulant solution, and the coagulant solution generally needs to undergo a heating process and a cooling process in sequence, the first package containing the coagulant solution is placed on the support member 13, so that the first package is located in the temperature-controlled chamber. And the second package is located outside the temperature control chamber.
The preparation process of the nutrient comprises the following steps: firstly, heating the coagulant solution in the first package body to enable the coagulant solution to reach a set temperature; introducing the nutrient solution and/or the additive solution in the second package into the first package; driving the second magnetic part 1 to drive the first magnetic part 7, the support part 13 and the first package body to move, so that the culture medium components in the first package body are fully shaken and mixed; the medium was cooled.
Wherein, can be provided with heater block and cooling part in the control by temperature change room, through controller control heater block and cooling part, and then carry out temperature regulation to the solution in the first packing.
Wherein, the temperature control room can adopt any shape and material, only need to form a relative confined space to satisfy the holding of first packing body and temperature regulation demand can. The heating and cooling components may take any of the structural forms disclosed in the prior art, as long as the heating and cooling requirements are met, respectively.
Wherein, the installation component can be for setting up the mounting groove outside the temperature control chamber, also can be for fixing the installing support at the temperature control chamber lateral wall, and concrete structural style is unrestricted, as long as can satisfy the installation of second packing body can. When the second packaging body is provided with the hanging hole, the mounting part can also be a hook positioned outside the temperature control chamber.
Furthermore, the culture medium preparation equipment also comprises a first communicating pipe, and the first communicating pipe is used for introducing components in the second package body at the installation part into the first package body in the temperature control room. The first communicating pipe is connected with an electric control pump, and the electric control pump pumps the components in the second packaging body into the first packaging body under the control of the controller.
Further, pipe joints are arranged at two ends of the first communication pipe, and the pipe joints are correspondingly arranged on the first packaging body and the second packaging body, so that the first communication pipe and the packaging bodies (including the first packaging body and the second packaging body) are connected. The pipe joint can realize the sealing connection between first connecting pipe and the packing body to can guarantee the quick assembly disassembly between first connecting pipe and the packing body.
The first end of first connecting pipe is located the control by temperature change indoor portion, and then when placing into the control by temperature change indoor portion with first packing body, can realize being connected between first packing body and the first connecting pipe. First communicating pipe second end is located the control by temperature change outdoor portion to be located installing component department, and then when installing the second packing body in installing component, can realize being connected between second packing body and the first communicating pipe.
In one embodiment, the number of the first communicating pipes is multiple, the number of the second packing bodies is one-to-one corresponding to the number of the first communicating pipes and is connected with the first communicating pipes, and the controller controls at least one of the first communicating pipes to be communicated. Therefore, when the number of the second packages is more than one, the controller injects the nutrient and/or the additive in at least one second package into the coagulant solution. Specifically, the controller may select the first communication pipe to be communicated based on the kind of the coagulant solution.
In one embodiment, the first packaging body is packaged through a second communicating pipe, namely the prepared culture medium is packaged through the second communicating pipe, and the controller controls the second communicating pipe to be connected and disconnected.
Wherein, first communicating pipe and second communicating pipe all can adopt the hose, and then the installation of being convenient for.
The above embodiments are merely illustrative of the present invention and are not to be construed as limiting the invention. Although the present invention has been described in detail with reference to the embodiments, it should be understood by those skilled in the art that various combinations, modifications and equivalents may be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention, and the technical solution of the present invention is covered by the claims of the present invention.

Claims (10)

1. A medium preparation apparatus, comprising:
a preparation chamber;
the support piece is positioned in the preparation chamber, can move in the preparation chamber, and is configured to be placed with a containing body containing a culture medium, and the containing body is a packaging bag;
the first magnetic piece is positioned in the preparation chamber and fixed at the bottom of the support piece;
the second magnetic part is positioned outside the preparation chamber and is correspondingly arranged below the first magnetic part;
the first driving part is connected with the second magnetic part and drives the second magnetic part to move, and the second magnetic part is suitable for driving the first magnetic part to move through a magnetic field;
the mounting seat is mounted on the bottom plate of the preparation chamber and is positioned below the supporting piece;
the mounting seat is connected with the supporting piece through at least three eccentric wheels, at least one of the eccentric wheels is a driving wheel, the driving wheel is connected with a first end of a rotating shaft, a second end of the rotating shaft penetrates through the mounting seat and then is connected with the first magnetic piece, or,
a connecting rod is arranged between the supporting piece and the mounting seat, one end of the connecting rod is fixedly connected with the first magnetic piece, the other end of the connecting rod is connected with the supporting piece, or,
the supporting piece and the cam are arranged between the mounting seats, and the first magnetic piece drives the cam to rotate so as to drive the supporting piece to move.
2. The media preparation apparatus of claim 1, wherein the first driving member is a rotary driving member, and the rotary driving member rotates the second magnetic member, and the second magnetic member rotates the first magnetic member.
3. The medium formulating device according to claim 2, wherein the mounting base is connected to the bottom plate through a turning shaft, a first stopper is provided on the mounting base, a second stopper is provided in the formulating chamber, and the first stopper and the second stopper are configured to: the first limiting piece and the second limiting piece are in limiting fit, and the mounting base is locked on the bottom plate; the first limiting part and the second limiting part are relieved from limiting, and the mounting base overturns around the overturning shaft under the action of external force.
4. The apparatus of claim 3, wherein the mounting base is a supporting plate, the first retaining member is a plug fixed to a bottom of the supporting plate, and the second retaining member is a socket connected to the second driving member and driven by the second driving member to move away from or fit around the plug.
5. The medium preparing apparatus according to claim 1, wherein the first driving member is mounted on a guide rod, and the first driving member is connected to and moved along an axial direction of the guide rod by an electric push rod.
6. The medium formulating device according to claim 1, wherein the first driving member is a driving motor connected to a magnet holder through a speed reducing member, the first magnetic member is a magnetic conductive plate, and the second magnetic member is a magnet fixed to the magnet holder.
7. The media preparation apparatus of claim 1, wherein the support is a mesh tray.
8. The media preparation apparatus of claim 1, wherein the preparation chamber is a temperature-controlled chamber.
9. The medium formulating device according to any one of claims 1 to 8, further comprising:
a mounting member, located outside the preparation chamber, configured to place a container containing a culture medium;
the containing body comprises a first containing body for containing coagulant solution and a second containing body for containing nutrient solution and/or additive solution;
the support piece is used for placing the first package body, and the mounting part is used for placing the second package body.
10. The media preparation apparatus of claim 9, further comprising:
and the first communication pipe is used for introducing the nutrient solution and/or the additive in the second packaging body into the first packaging body.
CN202010773695.2A 2020-08-04 2020-08-04 Culture medium preparation equipment Active CN111961573B (en)

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