CN109055184A - A kind of automatic transferred species turnover device of diphase blood culture flask - Google Patents
A kind of automatic transferred species turnover device of diphase blood culture flask Download PDFInfo
- Publication number
- CN109055184A CN109055184A CN201811026203.2A CN201811026203A CN109055184A CN 109055184 A CN109055184 A CN 109055184A CN 201811026203 A CN201811026203 A CN 201811026203A CN 109055184 A CN109055184 A CN 109055184A
- Authority
- CN
- China
- Prior art keywords
- bottle
- blood culture
- culture flask
- culture medium
- diphase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000009640 blood culture Methods 0.000 title claims abstract description 48
- 230000007306 turnover Effects 0.000 title claims abstract description 15
- 239000001963 growth medium Substances 0.000 claims abstract description 34
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 16
- 230000007246 mechanism Effects 0.000 claims abstract description 16
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229910052742 iron Inorganic materials 0.000 claims abstract description 8
- 239000002609 medium Substances 0.000 claims description 11
- 239000007787 solid Substances 0.000 claims description 11
- 239000008280 blood Substances 0.000 claims description 7
- 210000004369 blood Anatomy 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 238000009434 installation Methods 0.000 abstract description 2
- 241000894007 species Species 0.000 description 10
- 230000009471 action Effects 0.000 description 3
- 208000035143 Bacterial infection Diseases 0.000 description 2
- 208000022362 bacterial infectious disease Diseases 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 241001411320 Eriogonum inflatum Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 229920005549 butyl rubber Polymers 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/14—Rotation or movement of the cells support, e.g. rotated hollow fibers
Abstract
The present invention relates to a kind of flashing lightning magnetic field detectors, more particularly, to a kind of automatic transferred species turnover device of diphase blood culture flask.The automatic transferred species turnover device of the diphase blood culture flask, including pedestal, rotating disk, several cradles, top-pushing mechanism and sample disk, sample disk, cradle and diphase blood culture flask is driven integrally to rotate 360 ° by rotating disk, after rotating to designated position, the push rod for controlling top-pushing mechanism stretches out, the whole 90 ° of overturnings of two-phase phase Blood culture bottle on sample disk in cradle are realized, thus blind kind of purpose now.Postive stop baffle is equipped in bottle body, culture medium bracket is arranged between postive stop baffle, and culture medium bracket positions, is easy for installation；Suction iron plate is set at bottle cap center and magnet is set at culture medium cantilever tip center, culture medium bracket is fitted closely with bottle cap, and structure is more compact.The configuration of the present invention is simple is inoculated with convenient, high degree of automation.
Description
Technical field
The present invention relates to a kind of Blood culture bottles, more particularly, to a kind of automatic transferred species turnover device of diphase blood culture flask.
Background technique
In clinical medicine, the pathogen in patient's blood can be found out by blood culture, so as to carry out needle promptly and accurately
Treatment to property, so blood culture is one of inspection project important in clinical medicine laboratory.To find out the cause of disease in blood
The patient blood that venipuncture obtains usually is inoculated into Blood culture machine device and is cultivated, accurately to be diagnosed by bacterium
As a result.Due to the culture bottle price that import Blood culture machine device uses, existing laboratory generally uses diphase blood culture flask to carry out
Blood culture.But existing diphase blood culture flask, when transferred species, need to open bottle cap, inconvenient to use, and culture medium is easily and outside
Air contact in portion's pollutes.
Summary of the invention
In order to solve the above problem, the present invention provides a kind of automatic transferred species of diphase blood culture flask structurally reasonable, easy to use
Turnover device.
The technical solution adopted by the present invention is that: a kind of automatic transferred species turnover device of diphase blood culture flask, including pedestal and set
Set the rotating disk in portion in the base, it is characterised in that: further include several cradles that rotating disk surrounding is set, push cradle rotation
Top-pushing mechanism and sample disk for placing cradle, be placed with diphase blood culture flask, the rotation in several cradles
Disc spins drive sample disk, cradle and diphase blood culture flask integrally to be rotated；The top-pushing mechanism passes through solid in the middle part of rotating disk
It is scheduled on pedestal, cradle is provided with by shaft on the sample disk, the side in rotating disk, the push rod of the top-pushing mechanism
It stretches out, drives the cradle overturning being placed on sample disk.
Preferably, the L-shaped setting of diphase blood culture flask, bottom level section is for holding fluid nutrient medium；Top
Vertical section is arranged by rotating disk side, for holding solid medium.
Further, the diphase blood culture flask is closed in the bottle cap of bottle body and bottleneck and setting in bottle body including bottle body, lid
For holding the culture medium bracket of solid medium, the culture medium bracket is arranged in bottle body, the bottle cap and culture medium branch
Magnetic attracting device is equipped between top of the trellis, the bottle cap is movably arranged on the bottle mouth position of bottle body, and the Bottle cap top is equipped with sample tap；
The bottle body bottom is equipped with culture solution.
Further, the magnetic attracting device includes that the suction iron plate at bottle cap center is arranged in and is arranged in culture medium top of support
The magnet at end center.
Further, postive stop baffle is equipped in the bottle body, the culture medium bracket is inserted between postive stop baffle.
Further, the bottle cap is connected through a screw thread with bottle body.
The beneficial effect that the present invention obtains is: by rotating disk drive sample disk, cradle and diphase blood culture flask whole into
360 ° of row rotations, after rotating to designated position, the push rod for controlling top-pushing mechanism stretches out, and realizes the two-phase phase on sample disk in cradle
The whole 90 ° of overturnings of Blood culture bottle, thus blind kind of purpose now.Postive stop baffle is equipped in bottle body, the setting of culture medium bracket exists
Between postive stop baffle, culture medium bracket positions, is easy for installation；Sample tap, sample tap axis and bottle cap axis are equipped in Bottle cap top
Line is not conllinear, bottle cap rotation, and sample tap is corresponding with the solid medium on culture medium bracket.Whole overturning bottle body, can be completed
Transferred species operation, transferred species economy are convenient；It is arranged at bottle cap center and inhales iron plate and in culture medium cantilever tip center setting magnet, culture
Base bracket is fitted closely with bottle cap, and structure is more compact.The configuration of the present invention is simple is inoculated with convenient, high degree of automation.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is the structural schematic diagram of diphase blood culture flask；
Fig. 3 is the A-A cross-sectional view of Fig. 2；
Fig. 4 is the explosive view of Fig. 2.
Specific embodiment
The present invention will be further explained in the following with reference to the drawings and specific embodiments.
As shown in Figure 1, a kind of automatic transferred species turnover device of diphase blood culture flask of the invention, including pedestal 1, rotating disk 2,
The middle part of pedestal 1 is arranged in several cradles 3, top-pushing mechanism 6 and sample disk 4, rotating disk 2, and rotating disk 2 passes through runing rest component
21 connect with external impetus, and external impetus drives rotating disk 2 to rotate 360 ° by runing rest component 21.Sample disk 4 with
Cradle 3 corresponds, the outside in rotating disk 2, and cradle 3 is arranged on sample disk 4 by shaft, is placed on cradle 3
Diphase blood culture flask 5, sample disk 4, cradle 3 and diphase blood culture flask 5 are circumferentially uniformly distributed along rotating disk 2, and rotating disk 2 rotates,
Sample disk 4, cradle 3 and whole 360 ° of the progress of diphase blood culture flask 5 is driven at the uniform velocity to rotate.
Top-pushing mechanism 6 passes through to be fixed on pedestal 1 in the middle part of rotating disk 2, and top-pushing mechanism 6 uses cylinder or motor driven, control
Cylinder or motor processed, and then the movement of the push rod of top-pushing mechanism 6 is controlled, push rod stretches out, and the bottom of sample disk is pushed, to realize
Whole 90 ° of overturnings of diphase blood culture flask 5 on sample disk 5 in cradle 3, and then realize blind kind of purpose.
The cradle 3 on each sample disk 5 is allowed successively to suspend 6 corresponding position of top-pushing mechanism in rotating disk 2, using top
Cradle 3 on the sample disk 5 at this is made 90 ° of overturnings with diphase blood culture flask 4 by pushing mechanism 6, realizes blind kind, Zhou Erfu
Begin, cultivated by certain time, then by instrument photograph observation, output sample whether bacterial infection result.
As shown in Fig. 2, the L-shaped setting of diphase blood culture flask 5 in the present embodiment, bottom level section is for holding liquid training
Base 56 is supported, Upper vertical section is arranged by 2 side of rotating disk, for holding solid medium 531.
As shown in Figure 3-4, the diphase blood culture flask 5 in the present embodiment, the automatic report as microbial bacterial infection blood
A kind of consumptive material on sun, transferred species equipment, by PET material and high pressure polyethylene, permanent magnet, iron sheet and butyl rubber plug, sealing
Aluminium capping is composed by vacuumizing, including bottle body 51, bottle cap 52 and culture medium bracket 53, and the lid of bottle cap 52 is closed in bottle body 51
Bottle mouth position, connected by way of screw thread, culture medium bracket 53 be arranged in bottle body 51, solid medium 531 setting is training
It supports in base bracket 53, culture medium bracket 53 cannot be rotated in bottle body 51；Sample tap 521 is equipped at the top of bottle cap 52, at sample tap 521
Equipped with bottle stopper 55,521 axis of sample tap and 52 axis of bottle cap be not conllinear (i.e. deviation bottle cap center setting), when design, sample tap
521 are arranged at the top of bottle cap 52 in the outer part as far as possible, and bottle cap 52 rotates, and the solid on sample tap 521 and culture medium bracket 53 is trained
It is corresponding to support base 531.
In one embodiment, it is equipped with adsorbent equipment between bottle cap 52 and the top of culture medium bracket 53, in the present embodiment, absorption
Device includes the (use of magnet 542 that the suction iron plate 541 at 52 center of bottle cap is arranged in and 53 top center of culture medium bracket is arranged in
Permanent magnet), under the action of inhaling iron plate 541 and magnet 542, culture medium bracket 53 is bonded with 52 inside top of bottle cap.
In one embodiment, as shown in connection with fig. 2, postive stop baffle 511 is equipped in bottle body 51, culture medium bracket 53 is inserted into limit
Between the baffle 511 of position.When bottle cap 52 rotates, due to the effect of postive stop baffle 511, culture medium bracket 53 is limited in bottle body 51
It does not rotate.In the present embodiment, by whole 90 ° of the overturning of bottle body 51, the fluid nutrient medium 56 of 51 bottom of bottle body can be inoculated into culture
On the solid medium 531 of base bracket 53.
Culture medium bracket 53 is inserted between postive stop baffle 511 from 51 bottle mouth position of bottle body, is then screwed into bottle cap 52
On 51 bottleneck of bottle body, during bottle cap 52 screws in, under the action of inhaling iron plate 541 and magnet 542,53 quilt of culture medium bracket
It being drawn into 52 inside top of bottle cap and is bonded, under the action of postive stop baffle 511,531 normal direction of culture medium bracket is constant, from
And ensure that, in the case that bottle cap 52 is rotated by 90 °, the sample tap 521 on bottle cap 52 can be with the training on culture medium bracket 53
It is corresponding to support base 31.By whole 90 ° of the overturning of bottle body 51, that is, it can guarantee that the culture solution of 60ML can be in the case where reporting sun, realization most passes through
It helps simplest Mechanical Driven transferred species.
The cradle 3 for the doing flip-flop movement diphase blood culture flask 5 for being marked with patient's sample being placed in one by one on sample disk 4
On, it was covered with diphase blood culture flask 5 around rotating disk 2 one weeks；Then it is rotated in upper rotating disk 2, drives sample disk 4, cradle 3 and two-phase
Whole 360 ° of the progress of Blood culture bottle 5 at the uniform velocity rotates, and each diphase blood culture flask 5 top-pushing mechanism 6 on going to rotating disk 2 corresponds to position
It stops when the place of setting, the push rod of top-pushing mechanism 6 stretches out, and the cradle 3 for being loaded with diphase blood culture flask 5 is pushed to do 90 ° of overturnings, and realizing will note
There is the liquid culture medium 56 of patient's sample to submerge 531 surface of solid medium and realizes blind kind of purpose；After the completion of inoculation, top-pushing mechanism
6 pusher retracted resets, and is loaded with the cradle 3 of diphase blood culture flask 5 under 5 gravity of diphase blood culture flask before self- recoverage overturning
State, rotating disk 2 continue to rotate, and take turns to next bottle of position；Blind kind of above-mentioned overturning movement is successively repeated, by regular and journey
The culture environment of the electromechanical driving and configuration of sequence, and photograph monitoring effect, in instrument display screen or pass through hand automatically
Machine APP shows the report yang constipation fruit whether blood in diphase blood culture flask 5 or other body fluid are infected by bacterial.
Basic principle and key structural feature of the invention has been shown and described above.The present invention is not by the limit of examples detailed above
System, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements
It both falls in the range of claimed invention.The claimed scope of the invention is by appended claims and its equivalent
It defines.
Claims (6)
1. a kind of automatic transferred species turnover device of diphase blood culture flask, including pedestal (1) and the rotating disk being arranged in the middle part of pedestal (1)
(2), it is characterised in that: it further include setting in several cradles (3) of rotating disk (2) surrounding, the pusher of promotion cradle (3) rotation
Structure (6) and sample disk (4) for placing cradle (3) are placed with diphase blood culture flask (5), institute in several cradles (3)
Rotating disk (2) rotation is stated, sample disk (4), cradle (3) and diphase blood culture flask (5) is driven integrally to be rotated；The pusher
Structure (6) passes through to be fixed on pedestal (1) in the middle part of rotating disk (2), is provided with cradle (3), is located by shaft on the sample disk (4)
In the outside of rotating disk (2), the push rod of the top-pushing mechanism (6) stretches out, and the cradle (3) being placed on sample disk (4) is driven to turn over
Turn.
2. the automatic transferred species turnover device of diphase blood culture flask according to claim 1, it is characterised in that: the two-phase blood training
Bottle (5) L-shaped setting is supported, bottom level section is for holding fluid nutrient medium (56)；Upper vertical section is arranged by rotating disk side,
For holding solid medium (531).
3. the automatic transferred species turnover device of diphase blood culture flask according to claim 2, it is characterised in that: the two-phase blood training
It supports bottle (5), is closed including bottle body (51), lid interior solid for holding in bottle body (51) in the bottle cap (52) of bottle body (51) bottleneck and setting
The culture medium bracket (53) of body culture medium (531), culture medium bracket (53) setting is in bottle body (51), the bottle cap (52)
It is equipped with magnetic attracting device between culture medium bracket (53) top, the bottle cap (52) is movably arranged on the bottle mouth position of bottle body (51),
Sample tap (521) are equipped at the top of the bottle cap (52)；Bottle body (51) bottom is equipped with culture solution (56).
4. the automatic transferred species turnover device of diphase blood culture flask according to claim 3, it is characterised in that: the magnetic attracting device
In the suction iron plate (541) at bottle cap (52) center and the magnet (542) at culture medium bracket (53) top center is set including setting.
5. the automatic transferred species turnover device of diphase blood culture flask according to claim 3 or 4, it is characterised in that: the bottle body
(51) postive stop baffle (511) are equipped in, the culture medium bracket (53) is inserted between postive stop baffle (511).
6. the automatic transferred species turnover device of diphase blood culture flask according to claim 5, it is characterised in that: the bottle cap (52)
It is connected through a screw thread with bottle body (51).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811026203.2A CN109055184B (en) | 2018-09-04 | 2018-09-04 | Automatic seed turning device that changes of diphasic blood blake bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811026203.2A CN109055184B (en) | 2018-09-04 | 2018-09-04 | Automatic seed turning device that changes of diphasic blood blake bottle |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109055184A true CN109055184A (en) | 2018-12-21 |
| CN109055184B CN109055184B (en) | 2024-04-12 |
Family
ID=64758404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811026203.2A Active CN109055184B (en) | 2018-09-04 | 2018-09-04 | Automatic seed turning device that changes of diphasic blood blake bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109055184B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111961573A (en) * | 2020-08-04 | 2020-11-20 | 北京君立康生物科技有限公司 | Culture medium preparation equipment |
| CN113249208A (en) * | 2021-04-19 | 2021-08-13 | 珠海迪尔生物工程有限公司 | Blood sample culture detection workstation |
| CN114149919A (en) * | 2021-12-02 | 2022-03-08 | 鲁东大学 | A culture apparatus for cassava genetic information conversion |
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| GB926541A (en) * | 1958-06-05 | 1963-05-22 | Mini Agriculture & Fisheries | Improvements in or relating to the cultivation and sterilization of bacteria and the like |
| US5817507A (en) * | 1996-09-27 | 1998-10-06 | Becton Dickinson And Company | Blood culture apparatus having a bell-shaped drum |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111961573A (en) * | 2020-08-04 | 2020-11-20 | 北京君立康生物科技有限公司 | Culture medium preparation equipment |
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| CN113249208B (en) * | 2021-04-19 | 2022-10-28 | 珠海迪尔生物工程股份有限公司 | Blood sample culture detection workstation |
| CN114149919A (en) * | 2021-12-02 | 2022-03-08 | 鲁东大学 | A culture apparatus for cassava genetic information conversion |
| CN114149919B (en) * | 2021-12-02 | 2023-06-23 | 鲁东大学 | Culture device for cassava genetic information transformation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109055184B (en) | 2024-04-12 |
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