CN208218881U - A kind of novel T cell culture apparatus - Google Patents
A kind of novel T cell culture apparatus Download PDFInfo
- Publication number
- CN208218881U CN208218881U CN201820502775.2U CN201820502775U CN208218881U CN 208218881 U CN208218881 U CN 208218881U CN 201820502775 U CN201820502775 U CN 201820502775U CN 208218881 U CN208218881 U CN 208218881U
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- pedestal
- cell culture
- fixed
- support arm
- culture apparatus
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Abstract
The utility model provides a kind of novel T cell culture apparatus, including pedestal, annular groove-shaped pedestal is hinged on pedestal, it is fixed with support arm on pedestal, motor is fixed on support arm, is fixedly connected with crank in the shaft of motor, crank another end is rotatably connected to spin axis, spin axis is connect by elastic connecting component with pedestal, and the container for cell culture is fixed in pedestal, multiple magnetic beads being attached with for the antibody in conjunction with peripheral blood T cells are set in container；This culture apparatus rotates driving crank rotation by motor, the shaking of spin axis progress back and forth is driven with this, by the shaking of spin axis, support arm is torn as medium using rubber elastic layer gently to be shaken, solution in container comes into full contact with magnetic bead, and the elastic buffer of elastic layer improves the attachment time of contact of T cell, separating degree improves, and efficiency improves.
Description
Technical field
The utility model relates to a kind of novel T cell culture apparatus.
Background technique
Currently, peripheral blood lymphocytes culture is a kind of common Cell culture invitro technology, it is thin for periphery hemolymph
The separation of born of the same parents, the continuous amplification step of magnetic separation separation is cumbersome, low efficiency, and in T cell separation process, because of solution mobility
Difference is also easy to produce the phenomenon that T cell cannot come into full contact with magnetic bead, easily causes adsorption rate low.
Utility model content
In view of this, improving the attached of T cell the purpose of the utility model is to provide a kind of novel T cell culture apparatus
Time of contact, separating degree improve, efficiency improve, continuous cell Proliferation can be carried out.
In order to solve the above technical problems, the technical solution of the utility model is:
A kind of novel T cell culture apparatus, including pedestal, are hinged with annular groove-shaped pedestal on pedestal, fixed on pedestal
There is support arm, motor is fixed on support arm, crank is fixedly connected in the shaft of motor, crank another end is rotatably connected to
Spin axis, spin axis are connect by elastic connecting component with pedestal, and elastic connecting component includes multiple support arms, multiple support arm one end convergences
Fixed, other end intermittent is uniformly fixed with the notched wall of pedestal, and support arm pool side is fixed with tower-like rubber elastic layer, spin axis with
Elastic layer is fixedly connected, and the container for cell culture is fixed in pedestal, and multiple be attached with is set in container and is used for and peripheral blood
The magnetic bead for the antibody that middle T cell combines.
This culture apparatus rotates driving crank rotation by motor, drives the shaking of spin axis progress back and forth with this, relies on
The shaking of spin axis is torn support arm as medium using rubber elastic layer and is gently shaken, and the solution in container comes into full contact with magnetic bead, elastic layer
Elastic buffer, improve the attachment time of contact of T cell, separating degree improve, efficiency improve.
Further, the container top sets inlet, infusion port and air inlet, and container bottom sets going out by solenoid valve control
Liquid mouth, and rubber hose is connected on liquid outlet, rubber hose extends to outside base bottom, can be carried out continuously cell by container
Proliferation.
Further, it is provided with free bearing on the pedestal, free bearing is hinged with base bottom.
Further, electromagnet is provided in the side wall of the pedestal, when waste liquid is discharged, to adsorb magnetic bead.
Further, the support arm is 4, is fixed by bolt and pedestal notched wall, fixation.
The utility model has the beneficial effects that in conclusion the attachment time of contact of this culture apparatus raising T cell, separation
Degree improves, and efficiency improves, and can carry out continuous cell Proliferation.
Detailed description of the invention
Fig. 1: the structure chart of the utility model；
In figure: 1, pedestal；1.1, free bearing；2, support arm；3, pedestal；3.1, electromagnet；4, container；4.1, inlet；
4.2, infusion port；4.3, air inlet；4.4, liquid outlet；4.5, hose；5, support arm；5.1, elastic layer；6, motor；6.1, turn
Axis；6.2, crank；6.3, spin axis.
Specific embodiment
Below in conjunction with attached drawing, specific embodiment of the present utility model is described in further detail, so that the utility model skill
Art scheme is more readily understood and grasps.
Fig. 1 is a kind of one embodiment of novel T cell culture apparatus, including pedestal 1, is hinged with annular on pedestal 1
Channel-shaped pedestal 3, by the hinged pedestal 3 of free bearing 1.1, disposes container 4 in pedestal 3 as shown in Figure 1, free bearing 1.1 is arranged on pedestal 1,
Multiple magnetic beads being attached with for the antibody in conjunction with peripheral blood T cells are set in container 4, antibody is preferably that CD3 monoclonal is anti-
Body is fixed with support arm 2 on pedestal 1, is embodied, and support arm 2 is L-type, is fixed with motor 6 on support arm 2, it is preferable that
Motor 6 is the motor of subsidiary retarder, can carry out the adjusting of revolving speed, be fixedly connected with crank in the shaft 6.1 of motor 6
6.2,6.2 another end of crank is rotatably connected to spin axis 6.3, and spin axis 6.3 is connect by elastic connecting component with pedestal 3, elastic connection
Component includes multiple support arms 5, it is therefore preferable to which 4, interval equal angular is connected in 3 notched wall of pedestal, and 45 one end of support arm are converged
Poly- to fix, 5 pool side of support arm is fixed with tower-like rubber elastic layer 5.1, and spin axis 6.3 is fixedly connected with elastic layer 5.1, specific implementation
When, the direction that spin axis 6.3 is shaken is consistent with the usable rotational direction of pedestal 3, and rubber elastic layer 5.1 shakes as media buffer
Dynamics and intensity.
This culture apparatus is rotated by the rotation driving crank 6.2 of motor 6, drives the rolling of the progress of spin axis 6.3 back and forth with this
It is dynamic, by the shaking of spin axis 6.3, support arm 5 is torn for medium with rubber elastic layer 5.1 and is gently shaken, the solution in container 4 is abundant
Magnetic bead is contacted, the elastic buffer of elastic layer 5.1 improves the attachment time of contact of T cell, and separating degree improves, and efficiency improves.
As the further improvement of culture apparatus, inlet 4.1, infusion port 4.2 and air inlet are set at the top of the container 4
4.3,4 bottom of container sets the liquid outlet 4.4 by solenoid valve control, and rubber hose 4.5 is connected on liquid outlet 4.4, and rubber is soft
Pipe 4.5 extends to outside 3 bottom of pedestal, and the addition of blood specimen can be carried out by inlet 4.1, and infusion port 4.2 can carry out culture solution
Replacement, air inlet 4.3 is filled with required gas, and liquid outlet 4.4 excludes waste liquid, and the increasing of cell can be carried out continuously by container 4
It grows.
Preferably, electromagnet 3.1 is provided in the side wall of the pedestal 3, when waste liquid is discharged, to adsorb magnetic bead, into one
Step, can also be used plus the mode of strainer separates magnetic bead, and the present embodiment is no longer described in detail.
This culture apparatus improves the attachment time of contact of T cell, and separating degree improves, and efficiency improves, and can carry out continuous thin
Born of the same parents' proliferation.
Certainly, the above is only the specific application examples of the utility model, do not constitute and appoint to the protection scope of the utility model
What is limited.Any technical scheme formed by adopting equivalent transformation or equivalent replacement, all falls within the utility model rights protection model
Within enclosing.
Claims (5)
1. a kind of novel T cell culture apparatus, including pedestal, annular groove-shaped pedestal is hinged on pedestal, which is characterized in that bottom
It is fixed with support arm on seat, motor is fixed on support arm, crank is fixedly connected in the shaft of motor, crank another end turns
Dynamic to be connected with spin axis, spin axis is connect by elastic connecting component with pedestal, and elastic connecting component includes multiple support arms, multiple support arms
One end convergence is fixed, and other end intermittent is uniformly fixed with the notched wall of pedestal, and support arm pool side is fixed with tower-like caoutchouc elasticity
Layer, spin axis are fixedly connected with elastic layer, the container for cell culture are fixed in pedestal, multiple be attached with is set in container and is used for
The magnetic bead of antibody in conjunction with peripheral blood T cells.
2. the novel T cell culture apparatus of one kind according to claim 1, which is characterized in that the container top set into
Liquid mouth, infusion port and air inlet, container bottom sets the liquid outlet by solenoid valve control, and rubber hose is connected on liquid outlet,
Rubber hose extends to outside base bottom.
3. the novel T cell culture apparatus of one kind according to claim 1, which is characterized in that be provided on the pedestal
Free bearing, free bearing are hinged with base bottom.
4. the novel T cell culture apparatus of one kind according to claim 1, which is characterized in that in the side wall of the pedestal
It is provided with electromagnet.
5. the novel T cell culture apparatus of one kind according to claim 1, which is characterized in that the support arm is 4, is led to
Bolt is crossed to fix with pedestal notched wall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820502775.2U CN208218881U (en) | 2018-04-10 | 2018-04-10 | A kind of novel T cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820502775.2U CN208218881U (en) | 2018-04-10 | 2018-04-10 | A kind of novel T cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208218881U true CN208218881U (en) | 2018-12-11 |
Family
ID=64514718
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820502775.2U Active CN208218881U (en) | 2018-04-10 | 2018-04-10 | A kind of novel T cell culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208218881U (en) |
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2018
- 2018-04-10 CN CN201820502775.2U patent/CN208218881U/en active Active
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