CN208200999U - A kind of self-rocking T cell culture apparatus - Google Patents
A kind of self-rocking T cell culture apparatus Download PDFInfo
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- CN208200999U CN208200999U CN201820502160.XU CN201820502160U CN208200999U CN 208200999 U CN208200999 U CN 208200999U CN 201820502160 U CN201820502160 U CN 201820502160U CN 208200999 U CN208200999 U CN 208200999U
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- pedestal
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- rocking
- culture apparatus
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Abstract
The utility model provides a kind of self-rocking T cell culture apparatus, including pedestal, annular groove-shaped pedestal is hinged on pedestal, the container for cell culture is provided in base seat groove, buffer film is equipped between container and pedestal, support rod is symmetrically hinged in base bottom, the end that support rod is connect with pedestal is coated with silica gel frosted layer, is provided with electromagnet in pedestal side wall；Side is provided with electric putter on pedestal, and telescopic end is hinged with base bottom, and the other side is provided with spring, and the another end of spring is fixedly connected with base bottom；This culture apparatus is aided with spring using electric putter and gently shakes container, and T cell is made to come into full contact with magnetic bead, extends the contact attachment time, improves the separative efficiency of separation T cell, and automation is at low cost.
Description
Technical field
The utility model relates to a kind of self-rocking T cell culture apparatus.
Background technique
Currently, peripheral blood lymphocytes culture is a kind of common Cell culture invitro technology, it is thin for periphery hemolymph
The separation of born of the same parents, by taking Beads enrichment as an example, using the magnetic bead specific adsorption T lymphocyte of absorption CD3 or CD28 antibody in magnetic bead
Surface is then cultivated in Nostoc commune Vanch ware, transfers to culture dish Multiplying culture, cumbersome, low efficiency, and divide in T cell
From artificial jog container in the process, is needed, avoids generating the phenomenon that T cell cannot come into full contact with magnetic bead, easily causes adsorption rate low,
But for biggish container, artificial jog is taken time and effort, and effect is poor.
Utility model content
T cell is separated from culture apparatus, raising is shaken in view of this, the purpose of the utility model is to provide a kind of T cells
Separative efficiency, automation are at low cost.
In order to solve the above technical problems, the technical solution of the utility model is:
A kind of self-rocking T cell culture apparatus, including pedestal are hinged with annular groove-shaped pedestal on pedestal, set in base seat groove
It is equipped with the container for cell culture, buffer film is equipped between container and pedestal, is symmetrically hinged with support rod in base bottom,
The end that support rod is connect with pedestal is coated with silica gel frosted layer, is provided with electromagnet in pedestal side wall；Side is arranged on pedestal
There is electric putter, telescopic end is hinged with base bottom, and the other side is provided with spring, and the another end and base bottom of spring are fixed to be connected
It connects.
Further, the container top sets inlet, infusion port and air inlet, and container bottom sets going out by solenoid valve control
Liquid mouth, and rubber hose is connected on liquid outlet, rubber hose extends to outside base bottom, and multiple be attached with is set in container and is used for
The magnetic bead of antibody in conjunction with peripheral blood T cells.
Further, it is provided with free bearing on the pedestal, free bearing is hinged with base bottom.
Further, the buffer film is rubber-type buffer film.
Further, the control button of control electric putter is provided on the pedestal.
Further, the support rod is located at electric putter telescopic end and base bottom hinge joint and pedestal and pedestal hinge joint institute
On the straight line of formation and with base central point symmetry.
The utility model has the beneficial effects that this culture apparatus carries out the training of T cell using annular groove-shaped pedestal built-in receptacle
It supports, by the hinged pedestal of electric putter telescopic end, so that the rocking base in cell cultivation process, drives container, make to train in container
T cell in nutrient solution sufficiently contacts combination with the magnetic bead in container, to improve the efficiency of separation T cell, spring is used to slow down
The shaking speed of pedestal, another aspect auxiliary reset, using lower-powered electric putter be can reach shake liquid purpose, reduce at
This, support rod can keep the stabilization of pedestal at any time, to carry out the replacement of culture solution and the separation of waste liquid；In conclusion this culture
Device improves the separative efficiency of separation T cell, and automation is at low cost.
Detailed description of the invention
Fig. 1: the structure chart of the utility model；
In figure: 1, container；1.1, inlet；1.2, infusion port；1.3, air inlet；1.4, liquid outlet；1.5, hose；2, base
Seat；2.1, electromagnet；2.2, buffer film；2.3, spring；2.4, support rod；2.41, frosted layer；3, pedestal；3.1, free bearing；3.2,
Electric putter；3.21, telescopic end.
Specific embodiment
Below in conjunction with attached drawing, specific embodiment of the present utility model is described in further detail, so that the utility model skill
Art scheme is more readily understood and grasps.
Embodiment 1
As shown in Figure 1, a kind of self-rocking T cell culture apparatus, including pedestal 3, free bearing 3.1, free bearing are provided on pedestal 3
3.1 is hinged with 2 bottom of annular groove-shaped pedestal, and the container 1 for cell culture is provided in 2 slot of pedestal, it is preferable that container 1 with
It is equipped with buffer film 2.2 between pedestal 2, specifically, rubber-type buffer film 2.2 can be used in buffer film 2.2, on the one hand increases and rubs
It wipes, maintains the stability of container 1, on the other hand can adapt to the smaller or greater container 1 of size using the elasticity of rubber, improve suitable
Ying Xing is symmetrically hinged with support rod 2.4 on 2 bottom of pedestal, and the end that support rod 2.4 is connect with pedestal 2 is coated with silica gel frosted
Layer 2.41, the balance of pedestal 2 can be maintained using support rod 2.4 at any time, and silica gel frosted layer 2.41 improves stability, facilitates and trained
The replacement of nutrient solution and the separation of waste liquid are provided with electromagnet 2.1 in 2 side wall of pedestal, adsorb magnetic bead using electromagnet 2.1, are convenient for
Separate waste liquid；Side is provided with electric putter 3.2 on pedestal 3, and telescopic end 3.21 and 2 bottom of pedestal are hinged, passes through electric putter 3.2
Flexible to rock pedestal 2 along hinged rotation direction, the other side is provided with spring 2.3, and the another end of spring 2.3 and 2 bottom of pedestal are solid
Fixed connection, the intensity rocked using the elastic buffer pedestal 2 of spring 2.3 are come into full contact with magnetic bead with T cell and obtained longer attached
Binding time, improve the separating degree of T cell, improve efficiency.
Further, as shown in Figure 1, setting inlet 1.1, infusion port 1.2 and air inlet 1.3, container 1 at the top of the container 1
Bottom sets the liquid outlet 1.4 by solenoid valve control, and rubber hose 1.5 is connected on liquid outlet 1.4, and rubber hose 1.5 extends
To outside 2 bottom of pedestal, multiple magnetic beads being attached with for the antibody in conjunction with peripheral blood T cells are set in container 1, using into
Liquid mouth 1.1, infusion port 1.2 and air inlet 1.3 are infused to container 1, gas needed for replacing culture solution and supplying etc., can be after isolation
The continuous proliferation of cell is carried out, liquid outlet 1.4 can carry out the exclusion of waste liquid, to be continuously proliferated.
Further, the control button of control electric putter 3.2 is provided on the pedestal 3, convenient for manipulation.
Further, the support rod 2.4 be located at 3.2 telescopic end 3.21 of electric putter and 2 bottom hinge joint of pedestal and pedestal 2 with
3 hinge joint of pedestal is formed by straight line and with 2 center point symmetry of pedestal, stablizes.
This culture apparatus carries out the culture of T cell using 2 built-in receptacle 1 of annular groove-shaped pedestal, flexible by electric putter 3.2
3.21 hinged pedestals 2 are held, so that rocking base 2 in cell cultivation process, drive container 1, make the T in container 1 in culture solution
Cell sufficiently contacts combination with the magnetic bead in container 1, to improve the efficiency of separation T cell, spring 2.3 is used to slow down pedestal 2
Shaking speed, another aspect auxiliary reset, using lower-powered electric putter 3.2 be can reach shake liquid purpose, reduce at
This, support rod 2.4 can keep the stabilization of pedestal 2 at any time, to carry out the replacement of culture solution and the separation of waste liquid；In conclusion this
Culture apparatus improves the separative efficiency of separation T cell, and automation is at low cost.
Certainly, the above is only the specific application examples of the utility model, do not constitute and appoint to the protection scope of the utility model
What is limited.Any technical scheme formed by adopting equivalent transformation or equivalent replacement, all falls within the utility model rights protection model
Within enclosing.
Claims (6)
1. a kind of self-rocking T cell culture apparatus, including pedestal, annular groove-shaped pedestal is hinged on pedestal, which is characterized in that base
It is provided with the container for cell culture in seat slot, is equipped with buffer film between container and pedestal, it is symmetrical hinged in base bottom
There is support rod, the end that support rod is connect with pedestal is coated with silica gel frosted layer, is provided with electromagnet in pedestal side wall；On pedestal
Side is provided with electric putter, and telescopic end is hinged with base bottom, and the other side is provided with spring, the another end and base bottom of spring
It is fixedly connected.
2. a kind of self-rocking T cell culture apparatus according to claim 1, which is characterized in that the container top set into
Liquid mouth, infusion port and air inlet, container bottom sets the liquid outlet by solenoid valve control, and rubber hose is connected on liquid outlet,
Rubber hose extends to outside base bottom, and multiple magnetic being attached with for the antibody in conjunction with peripheral blood T cells are set in container
Pearl.
3. a kind of self-rocking T cell culture apparatus according to claim 1, which is characterized in that be provided on the pedestal
Free bearing, free bearing are hinged with base bottom.
4. a kind of self-rocking T cell culture apparatus according to claim 1, which is characterized in that the buffer film is rubber
Type buffer film.
5. a kind of self-rocking T cell culture apparatus according to claim 1, which is characterized in that be provided on the pedestal
Control the control button of electric putter.
6. a kind of self-rocking T cell culture apparatus according to claim 1, which is characterized in that the support rod is located at electricity
Push rod telescopic end and base bottom hinge joint and pedestal and pedestal hinge joint are formed by straight line and with base central point symmetry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820502160.XU CN208200999U (en) | 2018-04-10 | 2018-04-10 | A kind of self-rocking T cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820502160.XU CN208200999U (en) | 2018-04-10 | 2018-04-10 | A kind of self-rocking T cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208200999U true CN208200999U (en) | 2018-12-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201820502160.XU Active CN208200999U (en) | 2018-04-10 | 2018-04-10 | A kind of self-rocking T cell culture apparatus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109401970A (en) * | 2018-12-19 | 2019-03-01 | 南京比瑞生物科技有限公司 | A kind of multi-functional T bottles of full-automatic, totally-enclosed expansion of stem cells, induction Differentiation System |
| CN114149918A (en) * | 2021-11-29 | 2022-03-08 | 深圳中旭生物科技有限公司 | Methods and compositions for binding NK immune cells |
-
2018
- 2018-04-10 CN CN201820502160.XU patent/CN208200999U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109401970A (en) * | 2018-12-19 | 2019-03-01 | 南京比瑞生物科技有限公司 | A kind of multi-functional T bottles of full-automatic, totally-enclosed expansion of stem cells, induction Differentiation System |
| CN114149918A (en) * | 2021-11-29 | 2022-03-08 | 深圳中旭生物科技有限公司 | Methods and compositions for binding NK immune cells |
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