CN2780332Y - Device for seporating cell attached with magnatic nanometer particle - Google Patents
Device for seporating cell attached with magnatic nanometer particle Download PDFInfo
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- CN2780332Y CN2780332Y CN 200520041000 CN200520041000U CN2780332Y CN 2780332 Y CN2780332 Y CN 2780332Y CN 200520041000 CN200520041000 CN 200520041000 CN 200520041000 U CN200520041000 U CN 200520041000U CN 2780332 Y CN2780332 Y CN 2780332Y
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- container
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- separation
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Abstract
The utility model discloses a device for separating cells attached with magnatic nanometer particles, which comprises a container, a magnetic element and a solution cylinder, wherein the container is used for containing soft magnet powder, the magnetic element is arranged at the opening of the container and is used for covering the container, and the solution cylinder is provided with separating solution; a coil is wound on the magnetic element, and the container is submerged in the separating solution of the solution cylinder. The utility model fills the magnetic powder into the container, when the power is switched on, the cells with magnetism is attached to the outer tube wall of the container under the action of the magnetic field; after the cells are thoroughly attached, the device is taken out, and by using the principle on which cells attached with magnatic nanometer particles are repelled away from the tube wall under the action of the counter magnetic field, the utility model finally reaches the goal of separating the cells attached with magnetic nanometer particles. The utility model has the advantages of simple structure, convenient operation, easy cleaning, high separating efficiency, etc.
Description
Technical field
The utility model relates to a kind of separator, specifically a kind of device that can separate with the cell of magnetic nanoparticle.
Background technology
Magnetic nano-particle is widely used aspect biological and medical, and for example protein and enzyme is fixing, immunoassay, the purifying of RNA and DNA, cell separation and pharmaceutical carrier or the like.Utilize magnetic nano-particle do the cell separation system have easy and simple to handle, the equipment needed thereby cheapness, simultaneously separating rate is fast, the efficient height helps keeping advantages such as properties of samples, particularly biologically active.At present, the magnetic nano-particle utilization more and more widely.
Cell separation based on magnetic nano-particle is can be to the antibody or the other biological active material of specific cells identification in the connection of the surface of magnetic nano-particle.Therefore, magnetic nano-particle can combine with target cell.Under the effect of the magnetic field of adjustable magnetic separating device, with the cell detention that is combined on the magnetic nano-particle, thereby with the cell separation of other kind.Show that by Separation Research efficient reaches more than 30% to the CD34+ cell.
Therefore, design a kind of separator that can isolate the magnetic nanoparticle cell,, become the technical problem that those skilled in the art press for solution to satisfy the demand of now growing selective isolated cell.
Summary of the invention
Technical problem to be solved in the utility model provides a kind of separator that can isolate the magnetic nanoparticle cell, to satisfy the needs of above-mentioned prior art.
The technical scheme of the utility model technical solution problem is as follows:
A kind of separation is with the device of magnetic nanoparticle cell, it is characterized in that, comprise the container that is used to carrying the soft magnetism iron powder, be located at the fluid cylinder that vessel port place covers the magnetic element of container and separation solution is housed, be wound with coil on the described magnetic element, described container is dipped in the separation solution of fluid cylinder.When opening part covered the magnetic element disconnection of container, the cell of open pipe wall absorption was scolded in the opposing magnetic field.
The utility model operation principle is as follows:
At first magnetic is packed into during work in the container, then this device is inserted among the separated solution and on the interior coil that magnetic core arranged energising make the magnetic magnetization, the cell that has magnetic promptly can be adsorbed on the outer tube wall of device under the effect in magnetic field.To install taking-ups after absorption fully, be inserted in another clean solution after the cleaning again and cut off the electricity supply, cell quilt under the effect of opposing magnetic field of having nano magnetic particle this moment is scolded from tube wall.Finally reach the effect of cell separation.
By above disclosed technical scheme as can be known, the utlity model has simple in structure, easy to operate, easy cleaning and separative efficiency advantages of higher.
Description of drawings
Fig. 1 is the utility model structural representation;
Fig. 2 is specific embodiment structural representation of the utility model.
The specific embodiment
Further specify the utility model below in conjunction with accompanying drawing.
Referring to Fig. 1, the utility model separates the device with the magnetic nanoparticle cell, comprise the container 1 that is used to carrying the soft magnetism iron powder, be located at the fluid cylinder 7 that vessel port place covers the magnetic element 2 of container and separation solution is housed, be wound with coil 3 on the described magnetic element 2, described container 1 is dipped in the separation solution of fluid cylinder 7.
Described container can be for cylindric, cuboid and other shapes.
As further improvement of the utility model, develop the device of the isolated cell that to be applicable to the sample that a large amount of samples and cell extraction rate are higher on the basis of Fig. 1.As shown in Figure 2, described magnetic container outer wall is fixed with stirring arm 4.Stirring arm 4 is evenly distributed on container outer wall 1, and it is bar-shaped to make that the entire container outward appearance is special-shaped wolf's fang, also can be other shapes such as fan laminated.Be provided with top cover 5 between described magnetic element 2 and the vessel port, described magnetic element 2 is located on the top cover 5, is provided with the sealing ring 6 that covers container 1 opening in the top cover.This container embeds in the sealing ring that top cover rotates relatively in (or rotation is fixing) top cover, described sealing ring upper end is provided with rotating shaft, described top cover covers the opening part at fluid cylinder, also is provided with drive unit 8 on the described top cover, and the output shaft and the rotating shaft of described drive unit 8 interconnect.Container 1 rotates in fluid cylinder, and described drive unit is a drive motors, and sealing ring 6 related container 1 under the drive of motor 8 rotates together.Its magnetic absorption principle is identical with device noted earlier.Different is, rotation can make flow of solution reach purpose wolf's fang shape design simultaneously that absorption more fills and solution can be stirred more fully also improved simultaneously the area of magnetic absorption.
The above is the utility model preferred embodiment only, is not to be used for limiting the utility model practical range.All equalizations of being done according to the utility model scope change and modify, and are the utility model and contain.
Claims (5)
1. a separation is with the device of magnetic nanoparticle cell, it is characterized in that, comprise the container that is used to carrying the soft magnetism iron powder, be located at the fluid cylinder that vessel port place covers the magnetic element of container and separation solution is housed, be wound with coil on the described magnetic element, described container is dipped in the separation solution of fluid cylinder.
2. the device of separation magnetic nanoparticle cell according to claim 1 is characterized in that, described container is a cylindrical container.
3. separation according to claim 1 and 2 is characterized in that with the device of magnetic nanoparticle cell described container outer wall is fixed with stirring arm.
4. separation according to claim 3 is characterized in that with the device of magnetic nanoparticle cell described stirring arm is evenly distributed on container outer wall, and it is bar-shaped to make that the entire container outward appearance is special-shaped wolf's fang.
5. separation according to claim 4 is with the device of magnetic nanoparticle cell, it is characterized in that, be provided with top cover between described magnetic element and the vessel port, described magnetic element is located on the top cover, is provided with the sealing ring that covers vessel port in the top cover, and described container open end is embedded in the sealing ring of top cover, described sealing ring upper end is provided with rotating shaft, described top cover covers the opening part at fluid cylinder, also is provided with drive unit on the described top cover, and the output shaft and the rotating shaft of described drive unit interconnect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520041000 CN2780332Y (en) | 2005-04-20 | 2005-04-20 | Device for seporating cell attached with magnatic nanometer particle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520041000 CN2780332Y (en) | 2005-04-20 | 2005-04-20 | Device for seporating cell attached with magnatic nanometer particle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2780332Y true CN2780332Y (en) | 2006-05-17 |
Family
ID=36761531
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520041000 Expired - Fee Related CN2780332Y (en) | 2005-04-20 | 2005-04-20 | Device for seporating cell attached with magnatic nanometer particle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2780332Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104208906A (en) * | 2014-09-03 | 2014-12-17 | 上海市刑事科学技术研究院 | Simple and easy collection and elution device for magnetic nano enriching material and application method of simple and easy collection and elution device |
| CN104689906A (en) * | 2015-03-18 | 2015-06-10 | 延边大学 | In-column separation method for micro magnetic substances based on electromagnetic field |
| CN105331516A (en) * | 2015-12-01 | 2016-02-17 | 苏州浚惠生物科技有限公司 | Rare cell enrichment device and method |
| CN105536986A (en) * | 2016-02-01 | 2016-05-04 | 飞龙精工科技(苏州)有限公司 | Filtering system |
-
2005
- 2005-04-20 CN CN 200520041000 patent/CN2780332Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104208906A (en) * | 2014-09-03 | 2014-12-17 | 上海市刑事科学技术研究院 | Simple and easy collection and elution device for magnetic nano enriching material and application method of simple and easy collection and elution device |
| CN104689906A (en) * | 2015-03-18 | 2015-06-10 | 延边大学 | In-column separation method for micro magnetic substances based on electromagnetic field |
| CN104689906B (en) * | 2015-03-18 | 2016-09-07 | 延边大学 | The method separating minute yardstick magnetisable material in utilizing the post of electromagnetic field |
| CN105331516A (en) * | 2015-12-01 | 2016-02-17 | 苏州浚惠生物科技有限公司 | Rare cell enrichment device and method |
| CN105331516B (en) * | 2015-12-01 | 2018-03-06 | 苏州浚惠生物科技有限公司 | rare cell enrichment device and method |
| CN105536986A (en) * | 2016-02-01 | 2016-05-04 | 飞龙精工科技(苏州)有限公司 | Filtering system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |