CN104689906A - In-column separation method for micro magnetic substances based on electromagnetic field - Google Patents
In-column separation method for micro magnetic substances based on electromagnetic field Download PDFInfo
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- CN104689906A CN104689906A CN201510118336.2A CN201510118336A CN104689906A CN 104689906 A CN104689906 A CN 104689906A CN 201510118336 A CN201510118336 A CN 201510118336A CN 104689906 A CN104689906 A CN 104689906A
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Abstract
The invention discloses an in-column separation method for micro magnetic substances based on an electromagnetic field. The method comprises the steps of preparing magnetic nanoparticles of which the surfaces are modified by the co-precipitation method; charging flowing phase into a separating column; turning on a DC power supply to achieve fully electrifying so as to form the electromagnetic field in the separating column; pouring the modified magnetic nanoparticles into the separating column, wherein the magnetic nanoparticles are retained in the separating column; gradually reducing the intensity of the electromagnetic field, wherein the magnetic nanoparticles with relatively small size and low magnetic response capability can be moved out through the flowing phase, and the magnetic nanoparticles with relatively large size and high magnetic response capability can be retained in the separating column, so that the magnetic nanoparticles of different dimension can be separated; the intensity of the electromagnetic field is controlled to be 0 to 0.5T. With the adoption of the method, the micro magnetic substances with different size can be quickly separated; in addition, the separation method is simple, convenient and high in separating accuracy.
Description
Technical field
The present invention relates to a kind of separation method of minute yardstick magnetisable material, specifically utilize the method being separated minute yardstick magnetisable material in the post of electromagnetic field.
Background technology
In recent years, magnetic nanoparticle was in nano level magnetic material as one, had good magnetic response ability and superparamagnetism, can assemble location under stationary magnetic field.Simultaneously because magnetic nanoparticles size is little, surface electronic distribution is more makes that surface particles is active to be highly easy to be combined with other materials reduce surface energy, can modify various functional group by this point at surface particles.Therefore, magnetic nanoparticle is widely used in the fields such as biology, chemistry, medical treatment.The preparation method of magnetic nanoparticle mainly contains the methods such as coprecipitation, hydrothermal synthesis method, metal deoxidization, microemulsion synthesis method.The magnetic nanoparticle that these methods are synthesized inherently has the difference in size, and will in its finishing one deck functional group, also have the gathering between particle to occur during modifying functional group, cause the size distribution ranges of the magnetic nanoparticle prepared wider.The little particle of size cannot effectively response magnetic field cause cannot playing effective effect in follow-up application because self magnetic response ability is low, although and oversize magnetic nanoparticle for the responding ability in magnetic field by force because the easy comparatively greatly precipitation of its size is not easily applied.Therefore, screening has good magnetic response ability, and the magnetic nanoparticle that size is also moderate is very necessary.But the separation method at present for minute yardstick magnetisable material is still deficient, so need a kind of accurate, easy, quick and technology that can be continuously separated of exploitation.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art and provide a kind of separation method simply, conveniently, separation accuracy rate is high, and can be separated the method for minute yardstick magnetisable material in the post utilizing electromagnetic field of the minute yardstick magnetisable material of separation different size rapidly, continuously.
To achieve these goals, technical scheme of the present invention comprises the steps:
By the magnetic nanoparticle of coprecipitation preparation surface through modifying, magnetic nanoparticle particle diameter is
20n-500nm; Pass into mobile phase, opening power realizes fully energized thus forms electromagnetic field in splitter inside; Magnetic nanoparticle through modifying is injected into splitter inside, and magnetic nanoparticle is trapped in splitter inside under the effect of electromagnetic field; Finally reduce electromagnetic field intensity gradually, the weak magnetic nanoparticle of size less magnetic response ability will be brought out by mobile phase, the magnetic nanoparticle that size large magnetic response ability is stronger then can be retained in splitter inside, reaches the magnetic nanoparticle of separation different size with this.
Described electromagnetic field intensity modification scope is 0 ~ 0.5T.
Described power supply is dc source.
Owing to adopting technique scheme, the method tool being separated minute yardstick magnetisable material in the post of electromagnetic field that utilizes provided by the invention has the following advantages
:can the minute yardstick magnetisable material of quick separating different size, separation method is simple, convenient, is separated accuracy rate high, can also be continuously separated.
Accompanying drawing explanation
Figure
1the view of different size magnetic nanoparticle during for not applying electromagnetic field in the splitter in the present invention;
Figure
2after applying electromagnetic field in the splitter in the present invention, and the view of different size magnetic nanoparticle when reducing electromagnetic field gradually.
In the drawings, 1, small-size magnetic nano particle,
2, power supply,
3, coil,
4, splitter, 5, middle sized magnetic nano particle, 6, large scale magnetic nanoparticle.
Detailed description of the invention
:
As figure
1to figure
2shown in, the method being separated minute yardstick magnetisable material in the post of electromagnetic field that utilizes provided by the invention comprises the steps: to prepare the different size magnetic nanoparticle carrying out having modified in surface, and magnetic nanoparticles particle diameter exists
between 20nm to 500nm; Open pump and pass into mobile phase, opening power
2make coil
3energising thus at splitter
4inner formation electromagnetic field; Different size magnetic nanoparticle through modifying is injected into splitter
4inner, magnetic nanoparticle is because the effect in magnetic field is trapped in column interior, at this moment electromagnetic field intensity is reduced gradually, the weak small-size magnetic nano particle 1 of size little magnetic response ability will be brought out by mobile phase, it is inner that the middle sized magnetic nano particle 5 that size large magnetic response ability is strong and large scale magnetic nanoparticle 6 then can be retained in splitter 4, pass through said method, centering sized magnetic nano particle 5 is separated further with large scale magnetic nanoparticle 6 again, reaches the magnetic nanoparticle of separation different size with this.
During concrete operations, the magnetic nanoparticle of polymine for finishing, polymine is a kind of surfactant, can make the positive charge that magnetic nanoparticle surface band is a large amount of after modification, makes mutually to repel between magnetic nanoparticle to reduce copolymerization phenomenon as much as possible.First the magnetic nanoparticle that the size of polymine is different by finishing is injected into that splitter is inner and opening power generates electromagnetic field that magnetic nanoparticle is detained in splitter inside.At this time by regulating power supply to regulate electromagnetic field to make electromagnetic field change, the weak magnetic nanoparticle of size little magnetic response ability then will can be retained in splitter inside by the magnetic nanoparticle that mobile phase brings out size large magnetic response ability strong, reaches the magnetic nanoparticle of separation different size with this.
Claims (3)
1. utilize the method being separated minute yardstick magnetisable material in the post of electromagnetic field, it is characterized in that, it comprises the steps: to prepare the magnetic nanoparticle carrying out having modified in surface, and magnetic nanoparticle particle diameter is
20nm~
500nm; Open pump and pass into mobile phase, opening power makes coil electricity thus forms electromagnetic field in splitter inside; By through modification magnetic nanoparticle be injected into splitter inside, magnetic nanoparticle because magnetic field effect be trapped in column interior; Finally reduce electromagnetic field intensity gradually, the weak magnetic nanoparticle of size little magnetic response ability then will can be retained in splitter inside by the magnetic nanoparticle that mobile phase brings out size large magnetic response ability strong, reaches the magnetic nanoparticle of separation different size with this.
2. be separated the method for minute yardstick magnetisable material in the post utilizing electromagnetic field according to claim 1, it is characterized in that, described electromagnetic field intensity modification scope is 0 ~ 0.5T.
3. be separated the method for minute yardstick magnetisable material in the post utilizing electromagnetic field according to claim 1, it is characterized in that, described power supply is dc source.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510118336.2A CN104689906B (en) | 2015-03-18 | 2015-03-18 | The method separating minute yardstick magnetisable material in utilizing the post of electromagnetic field |
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| CN201510118336.2A CN104689906B (en) | 2015-03-18 | 2015-03-18 | The method separating minute yardstick magnetisable material in utilizing the post of electromagnetic field |
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| CN104689906A true CN104689906A (en) | 2015-06-10 |
| CN104689906B CN104689906B (en) | 2016-09-07 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105834004A (en) * | 2016-06-11 | 2016-08-10 | 兰州大学 | Separation method and device of nano particles |
| CN109746064A (en) * | 2019-01-28 | 2019-05-14 | 武汉纺织大学 | A kind of gradient magnetic micro-fluidic chip |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU544466A1 (en) * | 1971-09-29 | 1977-01-30 | Предприятие П/Я В-8857 | Magnetic separator |
| SU1181714A1 (en) * | 1983-04-29 | 1985-09-30 | Украинский Институт Инженеров Водного Хозяйства | Magnetic separator for separating ferromagnetic particles from fluid medium |
| CN1041703A (en) * | 1985-05-29 | 1990-05-02 | 乌克兰水利工程学院 | Device from the fluid medium separation of ferromagnetic materials |
| CN2780332Y (en) * | 2005-04-20 | 2006-05-17 | 上海师范大学 | Device for seporating cell attached with magnatic nanometer particle |
| CN101160176A (en) * | 2005-02-17 | 2008-04-09 | 纳幕尔杜邦公司 | Apparatus for magnetic field gradient enhanced centrifugation |
| CN101385994A (en) * | 2008-10-17 | 2009-03-18 | 中国海洋石油总公司 | Method for recovering magnetic particle in fluid and special recovery device |
| CN101842162A (en) * | 2007-10-29 | 2010-09-22 | 皇家飞利浦电子股份有限公司 | The method of splitter, piece-rate system, fractionating magnetic particles, the method for making splitter and the purposes of splitter |
| CN103769385A (en) * | 2012-10-18 | 2014-05-07 | 三星电机株式会社 | Cleaning device of magnetic nanoparticle and cleaning method using same |
-
2015
- 2015-03-18 CN CN201510118336.2A patent/CN104689906B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU544466A1 (en) * | 1971-09-29 | 1977-01-30 | Предприятие П/Я В-8857 | Magnetic separator |
| SU1181714A1 (en) * | 1983-04-29 | 1985-09-30 | Украинский Институт Инженеров Водного Хозяйства | Magnetic separator for separating ferromagnetic particles from fluid medium |
| CN1041703A (en) * | 1985-05-29 | 1990-05-02 | 乌克兰水利工程学院 | Device from the fluid medium separation of ferromagnetic materials |
| CN101160176A (en) * | 2005-02-17 | 2008-04-09 | 纳幕尔杜邦公司 | Apparatus for magnetic field gradient enhanced centrifugation |
| CN2780332Y (en) * | 2005-04-20 | 2006-05-17 | 上海师范大学 | Device for seporating cell attached with magnatic nanometer particle |
| CN101842162A (en) * | 2007-10-29 | 2010-09-22 | 皇家飞利浦电子股份有限公司 | The method of splitter, piece-rate system, fractionating magnetic particles, the method for making splitter and the purposes of splitter |
| CN101385994A (en) * | 2008-10-17 | 2009-03-18 | 中国海洋石油总公司 | Method for recovering magnetic particle in fluid and special recovery device |
| CN103769385A (en) * | 2012-10-18 | 2014-05-07 | 三星电机株式会社 | Cleaning device of magnetic nanoparticle and cleaning method using same |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105834004A (en) * | 2016-06-11 | 2016-08-10 | 兰州大学 | Separation method and device of nano particles |
| CN105834004B (en) * | 2016-06-11 | 2017-10-10 | 兰州大学 | The separation method and device of a kind of nano particle |
| CN109746064A (en) * | 2019-01-28 | 2019-05-14 | 武汉纺织大学 | A kind of gradient magnetic micro-fluidic chip |
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| Publication number | Publication date |
|---|---|
| CN104689906B (en) | 2016-09-07 |
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