CN202030763U - Rotary-type aerobic-type blood enriched culture bottle - Google Patents
Rotary-type aerobic-type blood enriched culture bottle Download PDFInfo
- Publication number
- CN202030763U CN202030763U CN 201120120846 CN201120120846U CN202030763U CN 202030763 U CN202030763 U CN 202030763U CN 201120120846 CN201120120846 CN 201120120846 CN 201120120846 U CN201120120846 U CN 201120120846U CN 202030763 U CN202030763 U CN 202030763U
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- China
- Prior art keywords
- bottle
- internal layer
- top cover
- layer pad
- center
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
- C12M27/12—Roller bottles; Roller tubes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Abstract
The utility model discloses a rotary-type aerobic-type blood enriched culture bottle for clinical medical inspection, which aims at solving the problems of the prior art that the sample feeding is convenient to operate, bacteria is easy to introduce when in operation, and the production cost is high. The rotary-type aerobic-type blood enriched culture bottle comprises a bottle body and a bottle cover, wherein an opening of the bottle body is provided with a tinfoil film, one side of a top cover is provided with a cover surface hole, an inner layer spacing piece which is matched with a bottle cover inner cavity is arranged inside the bottle cover inner cavity between the top cover and the tinfoil film, the inner layer spacing piece is divided into three areas in the clockwise direction, i.e. a closing area, a sample feeding area and a ventilation area, a rotary device is arranged on the center of the top cover, and the lower end of the rotary device passes through the center of the top cover to extend to the center of the inner layer spacing piece and to be fixedly connected with the inner layer spacing piece. The rotary-type aerobic-type blood enriched culture bottle has low production cost and is easily controlled to be bacteria-free, the sample feeding and the subcultivation are simple and fast to operate when the culture bottle is in use, and effects of bacteria prevention, closing and exchangeability of gas can be realized when the culture bottle is used for culturing.
Description
Technical field
The utility model relates to the medical apparatus field, particularly a kind ofly is used for the rotary aerobic-type blood enriched culture bottle that clinical medical inspection is used.
Background technology
It is one of most important Interventions Requested in the clinical medicine laboratory that blood increases the bacterium cultivation.Considerable disease, as various bacteria transmissible disease, pneumonia, meningitis, urinary system infection, peritonitis, osteomyelitis, postpartum one-level fever of unknown patient such as Sepsis, bacterial endocarditis, all may cause serious and critical systemic infections such as microbemia, septicemia because of bacterium enters blood, need find out the pathogenic bacteria in the blood as early as possible, in time give antibacterial therapy targetedly, therefore, hemoculture result accurately and timely is extremely important for clinical diagnosis and treatment.
Existingly be used for blood to increase the main tool that bacterium cultivates be the blood cultivation bottle.Blood culture bottle is to cultivate a kind of in the utensil, is widely used in the trace detection of invasive organism, accurately and timely provides cultivation results, for make a definite diagnosis microbemia, septicemia infects significant.The Blood culture bottle of clinical application at present mainly contains two big classes, be manual type and last type, not only price is higher wherein to go up the type Blood culture bottle, and need and the supporting use of instrumentation, and these instrumentations also mostly are external import, price is very expensive, and therefore manual type Blood culture bottle small-middle hospital at home has value for applications preferably.
The utility model of notification number CN2689223Y discloses a kind of blood cultivation bottle, this culturing bottle comprises seal bottle cap, sealing culture vessel, the sealing bottom of culture vessel is a chemoreceptor, in the middle of the container for being suitable for the various substratum of microorganism growth, above the substratum, internal tank has mixed gas.This culturing bottle bottle cap application of sample of when application of sample, need outwarding winding, operation inconvenience like this, and bring foreign material such as bacterium easily into and influence detected result, culturing bottle integral sealing, gas can not exchange, and are unsuitable for the cultivation of aerophil, dress chemoreceptor detection manufacturing cost height.
Summary of the invention
The purpose of this utility model is to solve the application of sample operation inconvenience that prior art exists, introduce bacterium during operation easily, the problem that production cost is high, a kind of rotary aerobic-type blood enriched culture bottle is provided, its production cost is low, be easy to control aseptic, application of sample during use, transferred species are simple and quick, can reach during cultivation every nectar close, the tradable effect of gas.
The technical scheme that its technical problem that solves the utility model adopts is: a kind of rotary aerobic-type blood enriched culture bottle, comprise bottle, bottle cap, the bottle mouth position of bottle is provided with the tinfoil film of sealed bottle body, be filled with enrichment medium in the bottle, described bottle cap comprises top cover and side cover, side cover is connected with the whorl of bottleneck of bottle, described top cover one side has a capping hole, be provided with the internal layer pad that matches with this inner chamber in the bottle cap inner chamber between described top cover and the tinfoil film, the internal layer pad is divided into three zones in the direction of the clock, three zones are followed successively by closed area, sample application zone and ventilating zone, the center of top cover is provided with a rotatable device, the center that the top cover center extends to the internal layer pad is passed in the rotatable device lower end, and is connected with the internal layer pad.Internal layer pad and bottle cap inner chamber match, chimeric with inner chamber, rotatable device passes top cover and is connected with the internal layer pad, like this, as long as rotation rotatable device top just can drive the internal layer pad and rotate, the internal layer pad is divided into closed area, sample application zone and ventilating zone, when closed area goes to below, capping hole, the utility model just is in air-tight state, when sample application zone goes to capping hole when below, but just application of sample of the utility model, the capping hole is less, only can hold the liquid-transfering gun head and pass through, both need not additionally to consume syringe during application of sample, can avoid extraneous bacterium to fall in the bottle again as far as possible and pollute, when ventilating zone went to below, capping hole, the utility model just can be ventilated.
As preferably, described rotatable device comprises knob and rotating shaft, and knob is fixed on the rotating shaft top, and the top cover center is passed in rotating shaft, and the center of the bottom of rotating shaft and internal layer pad is connected.Knob is used to drive rotating shaft and rotates, and is convenient to the application of force.
As preferably, the periphery of described knob is evenly equipped with three rotors, and angle is 120 degree between the adjacent rotor.Three rotors are convenient to rotatable device and are rotated, and rotating operation is convenient and strength-saving.
As preferably, described internal layer pad is parallel with top cover, the internal layer pad is circular, and closed area, sample application zone and ventilating zone are divided into the sector region of three homalographics with the internal layer pad, and three cut-off rules of closed area, sample application zone and ventilating zone are corresponding with three rotors respectively.Three cut-off rules of closed area, sample application zone and ventilating zone are corresponding with three rotors respectively, rotate rotor like this and can control closed area, sample application zone and ventilating zone more accurately to below, capping hole.
As preferably, described bottle cap is provided with protective cover, protective cover and bottle cap clamping.Protective cover is used to protect rotatable device.
As preferably, described sample application zone center has well, and the center of described ventilating zone has ventilating pit.Well is used for application of sample, and ventilating pit makes that bottle gas is commutative.
As preferably, be provided with criss-cross bracing frame in the described ventilating pit, bracing frame and ventilating pit inwall are connected, and bracing frame is provided with the bacteriological filtration film in closed vent hole.Bracing frame is convenient to place the bacteriological filtration film, and bracing frame plays and prevents that the bacteriological filtration film from coming off and fall into effect in the bottle, and bacteriological filtration membrane closure ventilating pit can prevent the intrusion of bacterium like this when gaseous interchange.
As preferably, described bacteriological filtration film upper surface and internal layer pad upper surface flush.Like this when the internal layer pad rotates, can prevent that the bacteriological filtration film from being rubbed and come off.
The beneficial effects of the utility model are: the rotary design of bottle cap, be easy to control aseptic, application of sample during use, transferred species are simple and quick, can reach during cultivation every nectar close, the tradable effect of gas.
Description of drawings
Fig. 1 is a kind of agent structure synoptic diagram of the present utility model;
Fig. 2 is a kind of sectional view of the utility model motor;
Fig. 3 is a kind of vertical view of the utility model internal layer pad.
Among the figure: 1, bottle, 2, bottle cap, 21, top cover; 22, capping hole, 3, the tinfoil film, 4, enrichment medium; 5, internal layer pad, 51, closed area, 52, sample application zone; 53, ventilating zone, 6, knob, 7, rotating shaft; 8, rotor, 9, protective cover, 10, well; 11, ventilating pit, 12, bracing frame, 13, the bacteriological filtration film.
Embodiment
Below by specific embodiment, and in conjunction with the accompanying drawings, the technical solution of the utility model is described in further detail.
Embodiment:
As Fig. 1; a kind of rotary aerobic-type blood enriched culture bottle shown in Figure 2; comprise bottle 1 and bottle cap 2; bottle cap 2 is provided with protective cover 9; protective cover 9 and bottle cap 2 clampings; bottle 1 is cylindrical; specification is 75mL; be filled with enrichment medium 4 in the bottle 1; enrichment medium 4 loading amounts are the 25mL/ bottle; the bottleneck of bottle 1 seals with tinfoil film 3; bottle cap 2 comprises top cover 21 and side cover; the side cover inwall has internal thread; the bottleneck outer wall has outside screw; side cover is connected with the whorl of bottleneck of bottle 1; top cover 21 right sides have the capping hole 22 of a circle; be provided with the internal layer pad 5 that matches with this inner chamber in bottle cap 2 inner chambers between top cover 21 and the tinfoil film 3; internal layer pad 5 is parallel with top cover 21; internal layer pad 5 is circular; internal layer pad 5 is divided into three zones in the direction of the clock; three zones are followed successively by closed area 51; sample application zone 52 and ventilating zone 53(Fig. 3); closed area 51; sample application zone 52 and ventilating zone 53 are divided into internal layer pad 5 sector region of three homalographics; sample application zone 52 centers have well 10; the center of ventilating zone 53 has ventilating pit 11; criss-cross bracing frame 12 is housed in the ventilating pit 11; bracing frame 12 is connected as a single entity with ventilating pit 11 inwalls; post the bacteriological filtration film 13 in closed vent hole 11 on the bracing frame 12; bacteriological filtration film 13 upper surfaces and internal layer pad 5 upper surface flush; the center of top cover 21 is provided with a rotatable device; rotatable device comprises knob 6 and rotating shaft 7; knob 6 is fixed in rotating shaft 7 tops; top cover 21 centers are passed in rotating shaft 7; the center of the bottom of rotating shaft 7 and internal layer pad 5 is connected; the periphery of knob 6 is evenly equipped with three rotors 8; angle is 120 degree, closed area 51 between the adjacent rotor 8; three cut-off rules of sample application zone 52 and ventilating zone 53 are corresponding with three rotors 8 respectively.
Before the use, bottle cap 2 and bottleneck need be screwed, closed area 51 be gone to 22 belows, capping hole make bottle 1 be in air-tight state, 121 ℃ of autoclavings, 15min takes out, and labelled, vacuum plastic sealing is stand-by.
During use, the nurse extracts the patients'blood sample, sends into the laboratory, gives laboratory technicians.After laboratory technicians places Bechtop to seal off blood enriched culture bottle; open culturing bottle protective cover 9; with 2 sterilizations of the careful wiping bottle cap of cotton ball soaked in alcohol; be rotated counterclockwise knob 6; well 10 is overlapped with capping hole 22; bottleneck tinfoil film 3 is broken by well 10 with sterilized liquid-transfering gun head again, drawn 2.5mL blood, splash in the bottle from well 10 with liquid-transfering gun.Behind the application of sample, be rotated counterclockwise knob 6 rapidly, ventilating pit 11 is overlapped with capping hole 22, be converted to the aerated culture state, the jolting culturing bottle makes blood fully contact substratum gently, and culturing bottle is put into incubator or 37 ℃ of cultivations of shaking table.Observe every day, if bacterial growth is arranged, then can place super clean bench to take a sample, the side cover of outwarding winding is opened whole bottle cap 2, get bacterium liquid transferred species and identify and drug sensitive experiment, or directly carry out PCR and identify bacterial classification, reached the purpose that goes out report fast in plate culture medium cultivation carrying out bacterium colony.
Above-described embodiment is a kind of preferable scheme of the present utility model, is not that the utility model is done any pro forma restriction, also has other variant and remodeling under the prerequisite that does not exceed the technical scheme that claim puts down in writing.
Claims (8)
1. rotary aerobic-type blood enriched culture bottle, comprise bottle (1), bottle cap (2), the bottle mouth position of bottle is provided with the tinfoil film (3) of sealed bottle body, be filled with enrichment medium (4) in the bottle, described bottle cap comprises top cover (21) and side cover, side cover is connected with the whorl of bottleneck of bottle, it is characterized in that: described top cover one side has a capping hole (22), be provided with the internal layer pad (5) that matches with this inner chamber in the bottle cap inner chamber between described top cover and the tinfoil film, the internal layer pad is divided into three zones in the direction of the clock, three zones are followed successively by closed area (51), sample application zone (52) and ventilating zone (53), the center of top cover is provided with a rotatable device, the center that the top cover center extends to the internal layer pad is passed in the rotatable device lower end, and is connected with the internal layer pad.
2. rotary aerobic-type blood enriched culture bottle according to claim 1, it is characterized in that: described rotatable device comprises knob (6) and rotating shaft (7), knob is fixed on the rotating shaft top, and the top cover center is passed in rotating shaft, and the center of the bottom of rotating shaft and internal layer pad is connected.
3. rotary aerobic-type blood enriched culture bottle according to claim 2 is characterized in that: the periphery of described knob is evenly equipped with three rotors (8), and angle is 120 degree between the adjacent rotor.
4. rotary aerobic-type blood enriched culture bottle according to claim 3, it is characterized in that: described internal layer pad is parallel with top cover, the internal layer pad is circular, closed area, sample application zone and ventilating zone are divided into the sector region of three homalographics with the internal layer pad, and three cut-off rules of closed area, sample application zone and ventilating zone are corresponding with three rotors respectively.
5. according to claim 1 or 2 or 3 or 4 described rotary aerobic-type blood enriched culture bottles, it is characterized in that: described bottle cap is provided with protective cover (9), protective cover and bottle cap clamping.
6. according to claim 1 or 2 or 3 or 4 described rotary aerobic-type blood enriched culture bottles, it is characterized in that: described sample application zone center has well (10), and the center of described ventilating zone has ventilating pit (11).
7. rotary aerobic-type blood enriched culture bottle according to claim 6 is characterized in that: be provided with criss-cross bracing frame (12) in the described ventilating pit, bracing frame and ventilating pit inwall are connected, and bracing frame is provided with the bacteriological filtration film (13) in closed vent hole.
8. rotary aerobic-type blood enriched culture bottle according to claim 7 is characterized in that: described bacteriological filtration film upper surface and internal layer pad upper surface flush.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120120846 CN202030763U (en) | 2011-04-22 | 2011-04-22 | Rotary-type aerobic-type blood enriched culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120120846 CN202030763U (en) | 2011-04-22 | 2011-04-22 | Rotary-type aerobic-type blood enriched culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202030763U true CN202030763U (en) | 2011-11-09 |
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ID=44893069
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120120846 Expired - Lifetime CN202030763U (en) | 2011-04-22 | 2011-04-22 | Rotary-type aerobic-type blood enriched culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202030763U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899242A (en) * | 2012-11-02 | 2013-01-30 | 中华人民共和国上海出入境检验检疫局 | Multifunctional test tube for cultivating bacteria and using method of multifunctional test tube |
| CN104928162A (en) * | 2015-06-23 | 2015-09-23 | 陈晋 | Novel biological safety sample preservation tube |
-
2011
- 2011-04-22 CN CN 201120120846 patent/CN202030763U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899242A (en) * | 2012-11-02 | 2013-01-30 | 中华人民共和国上海出入境检验检疫局 | Multifunctional test tube for cultivating bacteria and using method of multifunctional test tube |
| CN104928162A (en) * | 2015-06-23 | 2015-09-23 | 陈晋 | Novel biological safety sample preservation tube |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20111109 |
|
| CX01 | Expiry of patent term |