CN201261785Y - Two-phase multi-solid phase blood culture device - Google Patents

Two-phase multi-solid phase blood culture device Download PDF

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Publication number
CN201261785Y
CN201261785Y CN 200820148752 CN200820148752U CN201261785Y CN 201261785 Y CN201261785 Y CN 201261785Y CN 200820148752 CN200820148752 CN 200820148752 CN 200820148752 U CN200820148752 U CN 200820148752U CN 201261785 Y CN201261785 Y CN 201261785Y
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China
Prior art keywords
dividing plate
phase
opening
spaces
cultivation device
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Expired - Fee Related
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CN 200820148752
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Chinese (zh)
Inventor
朱国珍
孟云剑
刘亚锋
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Zhengzhou Fubosai biotechnology limited liability company
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ZHENZHOU BOSAI BIOTECH CO Ltd
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Priority to CN 200820148752 priority Critical patent/CN201261785Y/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses a diphasic multi-solid blood incubator, which comprises a closed container provided with an opening, a sealing plug matched with the opening is arranged in the position of the opening; one side of the inner chamber is divided into two spaces by a main baffle; one of the two spaces is divided into two or more than two spaces by one or more than one auxiliary baffle; fluid phase and solid phase culture medium can be added through opening once or more than twice, contaminations caused by repeatedly opening needed in the prior art is reduced, detections can be simultaneously performed on various bacteria, the use is very convenient, the work efficiency of operating staffs can be improved greatly, so as to be convenient for observation, and the sampling dead angle is reduced to the utmost extent; the diphasic multi-solid blood incubator is mainly used for blood culture, and also can be used for the culture of pathogenic germ in cerebrospinal fluid, pleural effusion and ascites and other various body fluids.

Description

How admittedly a kind of two-phase the blood cultivation device
Technical field
How admittedly the utility model belongs to technical field of medical instruments, relates to a kind of blood cultivation device, be specifically related to a kind of two-phase blood cultivation device.
Background technology
Hemoculture is one of most important Interventions Requested in the clinical medicine laboratory.Considerable disease, patient etc. as various bacteria transmissible disease, pneumonia, meningitis, urinary system infection, peritonitis, osteomyelitis, postpartum Sepsis, bacterial endocarditis one-level fever of unknown all may cause microbemia, septicemia because of bacterium enters blood.And this is serious and critical systemic infection, need find out the pathogenic bacteria in the blood as early as possible, and in time to give antibacterial therapy targetedly, therefore, hemoculture result accurately and timely is extremely important.
Blood culture bottle is to cultivate a kind of in the utensil, be widely used in the particularly microorganism of some severe nutrition of microorganism, trace detection as hemophilus influenzae, meningococcus, streptococcus pneumoniae, Brucella, defence line bacillus, core bar bacterium, Jin Shi bacillus etc., for accurately and timely providing the hemoculture result, make a definite diagnosis microbemia, whether septicemia infects significant.The Blood culture bottle that uses has two big classes at present, be manual type and last type, manual type is divided into biphasic or bipolar type and anaerobic type again, not only price is higher wherein to go up the type Blood culture bottle, and be and the supporting use of instrumentation, and these instrumentations are very expensive, and therefore hospital is widely used at home is manual type Blood culture bottle.
Existing commonly used is the two-sided Blood culture bottle of two-phase, comprise bottle, the middle and upper part of the opposing sidewalls of bottle outwards convexes to form solid medium the space is set, solid medium sticks on this space, once can put into two kinds of solid mediums, take a blood sample during use into bottle, under certain temperature, the cultivation of certain hour, on solid medium, can observe the growing state of bacterium.But also there are some shortcomings in the two-sided Blood culture bottle of this two-phase: loaded down with trivial details as making processes; Need open bottleneck three times when inwardly adding two kinds of solid phases and a kind of liquid phase, to at high temperature be earlier that liquid solid phase adds, be incorporated as second kind of liquid solid phase after waiting it to solidify again, after solidifying, second kind of solid phase add liquid phase again, three times opening pollutes easily, often has large batch of microbiological contamination phenomenon to occur; Solidus surface design is in the opposite flank of bottle, can not be directly from top view, because block mutually during from top view, be difficult for accurately observing the growth result; Exist the top at more dead angle, particularly outer lug to be difficult to sampling during to substratum bacterial classification culture transferring; The used material of bottle is a glass, and quality is heavy and broken easily, is inconvenient to the processing of transporting and using.
The utility model content
How admittedly the purpose of this utility model is to provide a kind of two-phase easy to use blood cultivation device.
For achieving the above object, the utility model is by the following technical solutions: how admittedly a kind of two-phase the blood cultivation device, comprise the encloses container that is provided with opening, opening part is provided with suitable with it airtight plug, one side of described container intracavity is separated by main dividing plate and is divided into two spaces, and one of them space is separated by one or more secondary dividing plates and is divided into two or more spaces.
Described main dividing plate and secondary dividing plate are arranged on the horizontal side of container intracavity, and main dividing plate and secondary dividing plate are intersected in same place.
Described opening is positioned on the relative side of horizontal side, and aperture position is corresponding with the intersection of major and minor dividing plate.
The height of described major and minor dividing plate is less than the height of container intracavity.
Described main dividing plate is arranged on the vertical side of container intracavity, and main dividing plate is divided into two spaces that communicate up and down with this side, and upper space is separated by one or more secondary dividing plates and is divided into two or more spaces.
Described opening is positioned at the top of container.
Described opening direction becomes 10~30 ° angle with the vertical side that major and minor dividing plate is set.
The height of described major and minor dividing plate is less than the width of container intracavity.
The free end of described main dividing plate is provided with baffle plate, and secondary dividing plate is arranged on the main dividing plate, and aperture position is corresponding with the free end of main dividing plate.
Described container material is a transparent plastics.
This practicality newly compared with prior art has the following advantages: (1) can add liquid phase and solid phase substratum under opening situation once or twice, has reduced the microbiological contamination phenomenon that needs repeatedly the opening application of sample to be caused in the prior art; (2) can add multiple solid phase simultaneously, simultaneously various bacteria be detected, very easy during use, improved operator's working efficiency greatly; (3) the solid phase substratum is arranged on the same side, does not block when observing, and has greatly guaranteed the accuracy that the observer judges the result; (4) certain opening direction is set, very convenient when making the culture transferring sampling, reduced the sampling dead angle to greatest extent; (5) adopt the transparent plastics material, light weight and be difficult for broken, made things convenient for transportation and use after processing.This two-phase how admittedly blood cultivation device is mainly used in cultivation to blood, and the pathogenic bacteria that also can be used for brains liquid, ascites pleural fluid and other multiple body fluid is cultivated.
Description of drawings
Fig. 1 is the stereographic map of embodiment 1;
Fig. 2 is the vertical view of Fig. 1;
Fig. 3 is the front view of embodiment 2;
Fig. 4 is the right view of Fig. 3;
Fig. 5 is the stereographic map of embodiment 3;
Fig. 6 is the vertical view of Fig. 5.
Embodiment
Embodiment 1: as depicted in figs. 1 and 2, the horizontal side 3 of encloses container 1 inner chamber is provided with main dividing plate 5, be divided into two spaces, one of them space is separated by secondary dividing plate 2 and secondary dividing plate 4 and is divided into three spaces, main dividing plate 5, secondary dividing plate 2 and secondary dividing plate 4 are intersected in same place, the opening 6 that is provided with suitable airtight plug 7 is arranged on the relative side of horizontal side 3, and the position of opening 6 is corresponding with the intersection of main dividing plate 5, secondary dividing plate 2 and secondary dividing plate 4, the height of main dividing plate 5, secondary dividing plate 2 and secondary dividing plate 4 is less than the height of container 1 inner chamber, and the material of container 1 is a transparent plastics.
During use, by opening 6 liquid phase and solid phase are partly added in the space that is separated by main dividing plate 5, secondary dividing plate 2 and secondary dividing plate 4, opening once just can be finished, cover airtight plug 7 then, getting blood is added in the liquid phase, rock or tilts to make liquid phase partly fully to contact with solid phase, observe and detect after cultivating certain hour, opening 6 is arranged on the intersection of main dividing plate 5, secondary dividing plate 2 and secondary dividing plate 4, does not have the sampling dead angle, and does not block obstacle when observing.
Embodiment 2: as shown in Figure 3 and Figure 4, main dividing plate 5 is set on the vertical side 8 of container 1 inner chamber, main dividing plate 5 is divided into two spaces that communicate up and down with vertical side 8, upper space is separated by secondary dividing plate 2 again, the opening 6 that is provided with suitable airtight plug 7 is positioned at the top of container 1, the angle of the direction of opening 6 and 8 one-tenth 10~30 ° of vertical sides, and main dividing plate 5 is less than the width of container 1 inner chamber, the height of secondary dividing plate 2 is less than the height of container 1 inner chamber, and the material of container 1 is a transparent plastics.
During use, by opening 6 liquid phase and solid phase are added, the space of main dividing plate 5 tops is the solid phase space, can once finish adding, again liquid phase is added after adding solid phase, main dividing plate 5 belows are the liquid phase space, opening can be finished the adding of solid phase and liquid phase for twice, cover airtight plug 7 then, getting blood is added in the liquid phase, rock or tilts to make liquid phase partly fully to contact with solid phase, observe and detect after cultivating certain hour, the angle of the direction of opening 6 and 8 one-tenths 10~30 ° of vertical sides has been eliminated the sampling dead angle, and do not blocked obstacle when observation.
Embodiment 3: as shown in Figure 5 and Figure 6, main dividing plate 5 is set on the vertical side 8 of container 1 inner chamber, main dividing plate 5 is divided into two spaces that communicate up and down with vertical side 8, the free end 9 of main dividing plate 5 is provided with baffle plate 10 upwards, form hemi-closure space, secondary dividing plate 2 is arranged on the main dividing plate 5, and the hemi-closure space of main dividing plate 5 tops is separated, the position of opening 6 that is provided with suitable airtight plug 7 is corresponding with the free end 9 of main dividing plate 5, and the material of container 1 is a transparent plastics.
During use, by opening 6 liquid phase and solid phase are added, the space that main dividing plate 5 tops are separated by secondary dividing plate 2 is the solid phase space, and the space of main dividing plate 5 belows is the liquid phase space, and opening once just can add solid phase and liquid phase simultaneously, cover airtight plug 7 then, getting blood is added in the liquid phase, rock or tilts to make liquid phase partly fully to contact, observe and detect behind the cultivation certain hour with solid phase, eliminate the sampling dead angle, and when observing, do not blocked obstacle.

Claims (10)

1, a kind of two-phase is consolidated the blood cultivation device more, comprise the encloses container that is provided with opening, opening part is provided with suitable with it airtight plug, it is characterized in that: a side of described container intracavity is separated by main dividing plate and is divided into two spaces, and one of them space is separated by one or more secondary dividing plates and is divided into two or more spaces.
2, two-phase as claimed in claim 1 is consolidated the blood cultivation device more, and it is characterized in that: described main dividing plate and secondary dividing plate are arranged on the horizontal side of container intracavity, and main dividing plate and secondary dividing plate are intersected in same place.
3, two-phase as claimed in claim 2 is consolidated the blood cultivation device more, and it is characterized in that: described opening is positioned on the relative side of horizontal side, and aperture position is corresponding with the intersection of major and minor dividing plate.
4, two-phase as claimed in claim 3 is consolidated the blood cultivation device more, and it is characterized in that: the height of described major and minor dividing plate is less than the height of container intracavity.
5, two-phase as claimed in claim 1 is consolidated the blood cultivation device more, it is characterized in that: described main dividing plate is arranged on the vertical side of container intracavity, main dividing plate is divided into two spaces that communicate up and down with this side, and upper space is separated by one or more secondary dividing plates and is divided into two or more spaces.
6, two-phase as claimed in claim 5 is consolidated the blood cultivation device more, and it is characterized in that: described opening is positioned at the top of container.
7, two-phase as claimed in claim 6 is consolidated the blood cultivation device more, and it is characterized in that: described opening direction becomes 10~30 ° angle with the vertical side that major and minor dividing plate is set.
8, two-phase as claimed in claim 7 is consolidated the blood cultivation device more, and it is characterized in that: the height of described major and minor dividing plate is less than the width of container intracavity.
9, two-phase as claimed in claim 5 is consolidated the blood cultivation device more, it is characterized in that: the free end of described main dividing plate is provided with baffle plate upwards, and secondary dividing plate is arranged on the main dividing plate, and aperture position is corresponding with the free end of main dividing plate.
10, consolidate the blood cultivation device as each described two-phase of claim 1 to 9, it is characterized in that: described container material is a transparent plastics more.
CN 200820148752 2008-08-26 2008-08-26 Two-phase multi-solid phase blood culture device Expired - Fee Related CN201261785Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200820148752 CN201261785Y (en) 2008-08-26 2008-08-26 Two-phase multi-solid phase blood culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200820148752 CN201261785Y (en) 2008-08-26 2008-08-26 Two-phase multi-solid phase blood culture device

Publications (1)

Publication Number Publication Date
CN201261785Y true CN201261785Y (en) 2009-06-24

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CN 200820148752 Expired - Fee Related CN201261785Y (en) 2008-08-26 2008-08-26 Two-phase multi-solid phase blood culture device

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023009772A1 (en) * 2021-07-30 2023-02-02 Corning Incorporated Baffle for microcavity cell culture vessels
US11732227B2 (en) 2018-07-13 2023-08-22 Corning Incorporated Cell culture vessels with stabilizer devices

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11732227B2 (en) 2018-07-13 2023-08-22 Corning Incorporated Cell culture vessels with stabilizer devices
WO2023009772A1 (en) * 2021-07-30 2023-02-02 Corning Incorporated Baffle for microcavity cell culture vessels

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C56 Change in the name or address of the patentee

Owner name: ZHENGZHOU RENFU BOSAI BIO-TECHNOLOGY LLC

Free format text: FORMER NAME: ZHENGZHOU BIOCELL BIOTECHNOLOGY CO., LTD.

CP01 Change in the name or title of a patent holder

Address after: 450016 No. 28, First Avenue, Zhengzhou economic and Technological Development Zone, Henan

Patentee after: Zhengzhou Fubosai biotechnology limited liability company

Address before: 450016 No. 28, First Avenue, Zhengzhou economic and Technological Development Zone, Henan

Patentee before: Zhenzhou Bosai Biotech Co., Ltd.

CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20090624

Termination date: 20170826

CF01 Termination of patent right due to non-payment of annual fee