CN204185488U - A kind of quick antibacterial test kit - Google Patents
A kind of quick antibacterial test kit Download PDFInfo
- Publication number
- CN204185488U CN204185488U CN201420614395.XU CN201420614395U CN204185488U CN 204185488 U CN204185488 U CN 204185488U CN 201420614395 U CN201420614395 U CN 201420614395U CN 204185488 U CN204185488 U CN 204185488U
- Authority
- CN
- China
- Prior art keywords
- test kit
- quick
- out console
- reagent bottle
- housed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The utility model provides a kind of quick antibacterial test kit, comprise sterile porous check-out console, moisture-heat preservation cultivates box, the reagent bottle of nutrient solution is housed, the reagent bottle of test organisms is housed, the reagent bottle of positive control antiseptic-germicide is housed, described sterile porous check-out console contains the detect aperture of more than 3 or 3 size equalizations, it is consistent, the homogeneous transparent material of thickness bottom detect aperture, described moisture-heat preservation is cultivated box and is made up of box body and lid, can keep flat 1-10 check-out console in box body.A kind of quick antibacterial test method of the present utility model and test kit, the antibacterial ability of test samples that can be quick, easy, and the advantage with high-throughput, low cost.
Description
Technical field
The utility model belongs to microbiological test field, specifically relates to a kind of quick antibacterial test kit.
Background technology
Antibacterial tests is a kind of test method for detecting and assessing antibacterial antiplaque agent effect, also can be used to screening new antibacterials and for bacterial detection resistance.
Existing standard antibacterial test method judges antiseptic-germicide performance based on agar plate calculating colony number changing conditions, and the test period is long.The operation steps of anti-(pressing down) the bacterium method for testing performance of anti-(pressing down) the bacterium product of this standard is as follows:
Bacterium (streptococcus aureus: ATCC6538 or intestinal bacteria: 8099 or ATCC25922) will be tested through 24h slant culture, culture PBS washes down, make bacteria suspension, the concentration required is: drip with 100 μ l and contrast in sample liquid (50X dilution) in 5ml, bacterial count recovered is 1 × 104 ~ 9 × 104cfu/ml.
Get sterilizing to cross by test liquid (5ml) and contrast sample liquid (5ml) (with sample homogeneous material, equal size, but not containing antiseptic-germicide, and through sterilising treatment) each 4 pipes.
Get above-mentioned bacteria suspension, 100 μ L (50X dilution) are dripped by test liquid and contrast sample liquid respectively each, Homogeneous phase mixing, start timing, effect 2, 5, 10, 20min, respectively sample liquid (0.5mL) is dropped in vitro (the 10X dilution) containing 5mLPBS with aseptic tweezer, abundant mixing, remake suitable dilution, then wherein 2 ~ 3 extent of dilution are got, draw 0.5mL respectively, be placed in two plates, pour into (30X dilution) with the cool nutrient agar 15mL to 40 ~ 45 DEG C, rotate plate, make it full and uniform, after agar solidification, upset is dull and stereotyped, cultivate 48h (bacterium) for 35 DEG C ± 2 DEG C and do viable bacteria enumeration.
Test repetition 3 times, is calculated as follows bacteriostasis rate: X=(A-B)/A × 100%.In formula: X is anti-(pressing down) bacterium rate, %; A--control sample average colony number; The tested sample average colony number of B--.
Judgement criteria: X >=50% ~ 90% is bacteriostasis rate, and product has bacteriostatic action, X >=90% is antibiotic rate, and product has anti-microbial effect.
This standard method test period is long, about needs 48h, and also have negative control group (not containing antiseptic-germicide) the bad control of colony number, general each negative control group plate colony number is relatively good between 100-900, too low or too high all will affect quantitative.Therefore, existing anti-(pressing down) bacterium test method is not suitable for intermediates or half-finished rapid detection on production line, is also not suitable for the high-throughput examination of a large amount of sample.
Summary of the invention
Problem to be solved in the utility model is to provide a kind of quick antibacterial test kit, the antibacterial ability of test samples that can be quick, easy, and the advantage with high-throughput, low cost.
For solving the problems of the technologies described above, the technical solution adopted in the utility model is: a kind of quick antibacterial test kit, comprise sterile porous check-out console, moisture-heat preservation cultivates box, the reagent bottle of nutrient solution is housed, the reagent bottle of test organisms is housed, the reagent bottle of positive control antiseptic-germicide is housed, described sterile porous check-out console contains the detect aperture of more than 3 or 3 size equalizations, it is consistent, the homogeneous transparent material of thickness bottom detect aperture, described moisture-heat preservation is cultivated box and is made up of box body and lid, can keep flat 1-10 check-out console in box body.
Preferably, described sterile porous check-out console contains 3 row 5 and arranges totally 15 detect aperture, and the left side 3 is classified as sample aperture, and the right 2 is classified as control wells, and the volume of each detect aperture is 0.1-0.5ml.
Preferably, described in test organisms is housed reagent bottle be reagent bottle with lid, bottle is built with slant agar substratum, and test organisms length is on slant agar substratum.
Preferably, described test organisms is streptococcus aureus, intestinal bacteria, Candida albicans or bacterium to be checked.
Preferably, described positive control antiseptic-germicide is water-soluble antibacterial agent.
Preferably, described nutrient solution is LB nutrient solution.
Preferably, described test kit also comprises the microbial culture pipe of more than 3 or 3 size equalizations, and microbial culture pipe is transparent Glass tubing or plastics tubing.
A kind of quick antibacterial test method, comprises the steps:
(1) test organisms liquid prepares: test organisms density is judged by the absorbancy detecting bacterium liquid under 470nm wavelength, and preferably, the OD470 of test organisms liquid reaches 0.5-0.8;
(2) test liquid and contrast liquid prepare;
(3) antibacterial reaction and termination;
(4) detect antibacterial effect: controlling at OD470 by initial for bacterium liquid OD value is 0.1-0.3, judged the antibacterial effect of sample by the absorbancy change detecting bacterium liquid under 470nm wavelength.
Preferably, quick antibacterial test method comprises the steps:
(1) test organisms liquid prepares: picking shakes through the test organisms or spend the night of 24h slant culture the test organisms that bacterium cultivates, and being diluted to OD470 with nutrient solution is 0.2-0.3, and then shaking table is cultured to OD470 and reaches 0.5-0.8;
(2) test liquid and contrast liquid prepare: each sample liquid to be tested of equivalent and the contrast liquid of preparing 0.2-1ml respectively, and contrast liquid comprises positive control solution and negative controls, and described negative controls is not containing the solvent of any antiseptic-germicide;
(3) antibacterial reaction and termination: the quantitative test bacterium liquid getting 0.01-0.1ml, respectively with each test liquid and contrast liquid and mix, test organisms liquid and test liquid or the mixed volume ratio contrasting liquid are 1:2-1:10; Timing after mixing, reaction 1-20min, then, centrifugal, remove supernatant, to add 0.2-5ml equivalent nutrient solution resuspended with termination reaction;
(4) antibacterial effect is detected: the resuspended bacterium liquid getting the equivalent of 0.1-1ml each sample group and control group respectively, doubly be placed in the different holes of sterile porous check-out console after OD470 is 0.1-0.3 with nutrient solution dilution 1-5, detect initial OD470, then cultivate bottom box at moisture-heat preservation and place aseptic hygenic towelette, sterile porous check-out console is placed on aseptic hygenic towelette, wave and culture 1-5h in 37 DEG C of shaking tables, detection per hour OD470, judges the antibacterial effect of each sample to be tested by the numerical value change of OD470.
A kind of quick antibacterial test method, comprises the steps:
(1) test organisms liquid prepares: the test organisms of picking through 24h slant culture or the test organisms of incubated overnight, is cultured to visible haze with shaking table after nutrient solution dilution 5-20 times;
(2) test liquid and contrast liquid prepare: each sample liquid to be tested of equivalent and the contrast liquid of preparing 0.5-2ml respectively, and contrast liquid comprises positive control solution and negative controls, and described negative controls is not containing the solvent of any antiseptic-germicide;
(3) antibacterial reaction and termination: get 0.05-0.2ml quantitative test bacterium liquid respectively with each sample liquid to be tested and contrast liquid and mix, reaction 1-20min, then adds 5-10 times of nutrient solution with termination reaction;
(4) antibacterial effect is detected: the equivalent bacterium liquid got respectively after the termination reaction of each sample group of 0.1-1ml and control group is placed in different bacterium culture tube, doubly stop to without obvious visible haze with nutrient solution dilution 1-5, then be placed in 37 DEG C of shaking tables and cultivate 1-2h or incubated at room temperature 18-24h, to observe and the turbidity contrasting liquid in each pipe judges the antibacterial effect of each sample to be tested.
The advantage that the utility model has and positively effect are: a kind of quick antibacterial test kit of the present utility model fast, simple, high-throughput, cost-saving, the antibacterial ability of sample can be detected in 1 to 5 hour, and up to a hundred and even thousands of samples can be detected simultaneously, and the amount of reagent used is few, saving testing cost, is suitable for basic unit or professional institution carries out antibacterial tests, microbiological test, new anti-bacterial agent examination and bacterial drug resistance detection.
Accompanying drawing explanation
Fig. 1 is the front view of the utility model sterile porous check-out console;
Fig. 2 is the vertical view of the utility model sterile porous check-out console;
Fig. 3 is the structural representation of the utility model quick antibacterial test kit;
Fig. 4 compares OD470 and OD600 and bacterial density graph of a relation in the utility model embodiment 3.
In Fig. 1-Fig. 3: 1. sterile porous check-out console, 2. detect aperture, 3. moisture-heat preservation cultivates box, 4. lid, 5. box body, 6. aseptic hygenic towelette, is 7. equipped with the reagent bottle of nutrient solution, and the reagent bottle of test organisms is 8. housed, 9. nutrient agar, 10. bacterium colony, 11. reagent bottles that positive control antiseptic-germicide is housed, 12. cartons.
Embodiment
Below in conjunction with specific embodiment, the utility model is described in further detail, but do not limit protection domain of the present utility model.
Embodiment 1
As shown in Figure 1-Figure 3, a kind of quick antibacterial test kit, comprising:
(1) sterile porous check-out console 1 (15 hole): transparent plastics mold obtains, the detect aperture 2 of totally 15 size equalizations is arranged containing 3 row 5, the left side 3 is classified as sample aperture, the right 2 is classified as control wells, the volume of each detect aperture 2 is 0.1-0.5ml, and bottom detect aperture, thickness is consistent, homogeneous.
(2) moisture-heat preservation cultivates box 3 (1): be made up of box body 5 and lid 4, its size enough keeps flat 1 check-out console 1, is transparent plastics material.
(3) reagent bottle 7 (30ml) of nutrient solution is housed: one bottle of LB nutrient solution.
(4) reagent bottle 8 (1 bottle) of test organisms is housed: long LB nutrient agar 9 surface on inclined-plane of intestinal bacteria 10 (ATCC25922).
(5) reagent bottle 11 (1ml) of positive control antiseptic-germicide is housed: 0.1% CHG.
Embodiment 2
A preparation method for quick antibacterial test kit, comprises the steps:
(1) prepare sterile porous check-out console 1: by molding shown in Fig. 2, then obtain with known method transparent plastics mold.Check-out console 1 forms containing 3 row 5 row, 15 detect aperture 2, and left-hand component 3X3 hole (S1, S2, S3 tri-groups) is sample aperture, for detecting antibacterial sample; (D1, D2 two groups) is control wells in right-hand component 2X3 hole, for detecting positive control and negative control antiseptic-germicide; The volume of each detect aperture 2 is about 0.35ml; Use aseptic plastic bag hermetic package, for subsequent use.
(2) nutrient solution is prepared: be divided in the vial of 50ml after being prepared by LB nutrient solution, sealed storage after every bottle of 30ml high pressure sterilization, for subsequent use.
(3) test organisms is prepared: after LB nutrient agar high pressure sterilization, cool to about 50 DEG C, be distributed in the little wide mouth glass bottle of the 3ml of Aseptic sterilisation, every bottle of 1.5ml, tiltedly put, leave standstill to nutrient agar and solidify, for subsequent use; By intestinal bacteria (ATCC25922) line at agar media surface, cultivate for 37 DEG C and grow to a large amount of bacterium colony 10 groups for 2 days, refrigerate for subsequent use.
(4) positive control antiseptic-germicide is prepared: get commercial 20% CHG (CAS#:LRAA1627) 0.1ml, join in the sterile distilled water of 20ml, mixing, again after 0.2 μ filter membrane sterile filtration, by every bottle of 1ml on super clean bench, be divided in the 2ml phial that high pressure sterilization crosses, cover tightly for subsequent use.
(5) prepare moisture-heat preservation and cultivate box 3: as shown in Figure 3, be made up of box body 5 and lid 4, its size, enough to keep flat 1 check-out console 1 for degree, obtains with transparent plastics; Be used for during antibacterial tests holding sterile porous check-out console 1, and at the aseptic hygenic towelette 6 of sterile porous check-out console 1 underlay in case bacterium liquid evaporation in check-out console 1, cover lid is placed in shaking table and is incubated wave and culture.
(6) moisture-heat preservation that the reagent bottle 11 that positive control antiseptic-germicide is housed that the reagent bottle 8 that test organisms is housed that the reagent bottle 7 that nutrient solution is housed sterile porous check-out console 1 obtained for above-mentioned steps (1), step (2) obtained, step (3) obtain, step (4) obtain, step (5) obtain is cultivated box 3 and is respectively got portion, merging is contained in carton 12, just becomes quick antibacterial test kit.
Embodiment 3
The relatively effect of OD470 and OD600 when bacterial detection density:
Get 0.5ml intestinal bacteria (ATCC25922) to spend the night bacterium liquid, with after the dilution of the LB nutrient solution of 5ml on 37 DEG C of shaking tables wave and culture 3h, get the LB nutrient solution mixing of this bacterium liquid 0.1ml and 0.1ml, then, multiple proportions gradient dilution is made 7 times again with LB nutrient solution, last only LB nutrient solution is not containing bacterium, then the bacterium liquid of this serial dilutions (8 parts) is forwarded in enzyme bidding quotation, by microplate reader each OD value organizing bacterium liquid after 470nm and 600nm detects gradient dilution respectively, result is as shown in table 1 and Fig. 4.
OD450 and OD600 result is detected under table 1. different bacterium liquid extent of dilution
| Strain density | 1 | 0.5 | 0.25 | 0.125 | 0.063 | 0.031 | 0.016 | 0.000 |
| 470nm | 0.637 | 0.414 | 0.246 | 0.163 | 0.111 | 0.083 | 0.069 | 0.047 |
| 600nm | 0.388 | 0.246 | 0.151 | 0.085 | 0.066 | 0.051 | 0.045 | 0.043 |
Experiment results proved: the bacterial density in bacterium liquid can be measured with the wavelength (OD470nm) of 470nm and the wavelength (OD600nm) of 600nm, and both OD values are all proportional with strain density, namely the extension rate of bacterium liquid is higher, the density of bacterium is lower, and the value of OD470 and OD600 is less.But, bacterial density ratio 600nm better effects if in bacterium liquid is measured with the wavelength of 470nm, because, for measuring the bacterial density of certain limit, OD470nm has larger window and sensitiveer, when that is bacterial density slightly changes than OD600nm, the change of OD470nm value than the change of OD600nm value greatly, especially, when strain density is lower, whether 600nm does not also detect has bacterium, and 470nm can detect.
Embodiment 4
Detect the antibacterial effect of chitosan by quick antibacterial test method, comprise the steps:
(1) test organisms liquid prepares: picking bacterium colony from intestinal bacteria (ATCC25922) the bacterium colony group the agar plates of a deposit is also transferred in the LB nutrient solution of 5ml, 37 DEG C are spent the night and shake bacterium, next day, getting the bacterium LB nutrient solution 5ml that spends the night in right amount, to be diluted to OD470 be 0.2-0.3, and then 37 DEG C of shaking tables are cultivated recovery and reached 0.8 to OD470;
(2) sterile porous check-out console 1 prepares: get a sterile porous check-out console 1, with the inside and outside sterilization of 70% ethanol spray hole, then air-dry on super clean bench, for subsequent use;
(3) test liquid and contrast liquid prepare: the sample to be tested preparing 1ml respectively: 0.1% chitosan solution and 0.05% chitosan solution, and adjust pH to 7.2, with distilled water preparation positive control solution 0.1% chlorhexidine gluconic acid solution 1ml, and negative controls PBS1ml; Sample liquid to be tested and the positive, negative controls 65 DEG C of water-bath 20min are carried out pasteurize;
(4) antibacterial reaction and termination: get 4 little centrifuge tubes of 1.5ml, mark #1, #2, #3, #4, add 0.2ml test organisms liquid in each pipe, again respectively successively to #1,0.05% chitosan solution of 0.5ml, 0.1% chitosan solution, positive control solution, negative controls is added in #2, #3, #4 pipe, hybrid reaction 20min, then, centrifugal, removing supernatant, add the LB nutrient solution of 0.5ml to each pipe, resuspended bacterium is with termination reaction;
(5) antibacterial effect is detected: get the multi-well assay plate that pre-sterilization is crossed, get the resuspended bacterium liquid of each sample of 0.2ml previous step respectively, be added in S1 respectively, S2, D1, in D2 hole, the LB nutrient solution (not containing bacterium) of equivalent is added in S3 hole, often organize work 2 (T1, T2), detect initial T0 bacterial density (OD470), then this multi-well assay plate being placed in moisture-heat preservation cultivates in box, in the aseptic hygenic towelette of check-out console underlay (in case bacterium liquid evaporation in check-out console), cover moisture-heat preservation and cultivate lid, wave and culture in 37 DEG C of shaking tables, detection about per hour bacterial density (OD470), detected result is as shown in table 2.
Table 2. is bacterial density (OD470) changing conditions in time after different sample preparation
Detected result shows:
D2: the test organisms OD470 value of negative control (without antiseptic-germicide) raises with incubation time, illustrates that test organisms also lives;
D1: positive control (chlorhexidine 0.1%) completely inhibit bacterium and increases (OD470 value is constant), illustrates that test organisms is killed;
S3:LB (aseptic, without antiseptic-germicide) does not have bacterium to increase (OD470=is blank) completely, and sterile contamination is described.
S2: chitosan 0.1% also basic anti-bacteria completely increases (OD470 value is constant), illustrates that 0.1% chitosan has remarkable anti-microbial effect;
S1: chitosan 0.05% only bacterium increases slack-off a little (rising of OD470 value), illustrates that 0.05% chitosan only has slight bacteriostatic action.
The present embodiment result shows: the antibacterial effect of chitosan is concentration dependent, has remarkable antibacterial effect when chitosan concentration is 0.1%, loses antibacterial ability when concentration reduces to 0.05%.Thus prove that quick antibacterial test method of the present utility model is suitable for the antibacterial effect of rapid detection antiseptic-germicide.
Above preferred embodiment of the present utility model has been described in detail, but described content being only preferred embodiment of the present utility model, can not being considered to for limiting practical range of the present utility model.All equalizations done according to the utility model application range change and improve, and all should still belong within patent covering scope of the present utility model.
Claims (7)
1. a quick antibacterial test kit, it is characterized in that: comprise sterile porous check-out console, moisture-heat preservation cultivates box, the reagent bottle of nutrient solution is housed, the reagent bottle of test organisms is housed, the reagent bottle of positive control antiseptic-germicide is housed, described sterile porous check-out console contains the detect aperture of more than 3 or 3 size equalizations, it is consistent, the homogeneous transparent material of thickness bottom detect aperture, described moisture-heat preservation is cultivated box and is made up of box body and lid, can keep flat 1-10 check-out console in box body.
2. a kind of quick antibacterial test kit according to claim 1, is characterized in that: described sterile porous check-out console contains 3 row 5 and arranges totally 15 detect aperture, and the left side 3 is classified as sample aperture, and the right 2 is classified as control wells, and the volume of each detect aperture is 0.1-0.5ml.
3. a kind of quick antibacterial test kit according to claim 1 and 2, is characterized in that: described in test organisms is housed reagent bottle be reagent bottle with lid, bottle is built with slant agar substratum, and test organisms length is on slant agar substratum.
4. a kind of quick antibacterial test kit according to claim 3, is characterized in that: described test organisms is streptococcus aureus, intestinal bacteria, Candida albicans or bacterium to be checked.
5. a kind of quick antibacterial test kit according to claim 1 and 2, is characterized in that: described positive control antiseptic-germicide is water-soluble antibacterial agent.
6. a kind of quick antibacterial test kit according to claim 1 and 2, is characterized in that: described nutrient solution is LB nutrient solution.
7. a kind of quick antibacterial test kit according to claim 1 and 2, it is characterized in that: described test kit also comprises the microbial culture pipe of more than 3 or 3 size equalizations, microbial culture pipe is transparent Glass tubing or plastics tubing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420614395.XU CN204185488U (en) | 2014-10-22 | 2014-10-22 | A kind of quick antibacterial test kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420614395.XU CN204185488U (en) | 2014-10-22 | 2014-10-22 | A kind of quick antibacterial test kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204185488U true CN204185488U (en) | 2015-03-04 |
Family
ID=52617500
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420614395.XU Active CN204185488U (en) | 2014-10-22 | 2014-10-22 | A kind of quick antibacterial test kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204185488U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111304057A (en) * | 2020-03-23 | 2020-06-19 | 浙江圣达生物研究院有限公司 | Kit and method for evaluating antibacterial effect based on microorganism test piece |
-
2014
- 2014-10-22 CN CN201420614395.XU patent/CN204185488U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111304057A (en) * | 2020-03-23 | 2020-06-19 | 浙江圣达生物研究院有限公司 | Kit and method for evaluating antibacterial effect based on microorganism test piece |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20150267163A1 (en) | Method for inducing bacteria to enter into viable but nonculturable state | |
| CN109845761B (en) | Application of lysozyme two-dimensional nano-film as antibacterial material | |
| JP7084719B2 (en) | Microbial growth medium and method of using it | |
| CN104611403A (en) | Food and tableware coliform bacteria rapid detection scrip preparation and application | |
| CN104293661A (en) | Quick antibacterial test method and kit | |
| CN104313118A (en) | Antibacterial efficacy detection method of diatom antibacterial material | |
| CN204185488U (en) | A kind of quick antibacterial test kit | |
| CN105524869A (en) | In-vitro dental plaque biofilm model and preparation method therefor | |
| CN106967779B (en) | Screening culture medium suitable for wide drug-resistant pseudomonas aeruginosa and preparation method thereof | |
| CN103173517A (en) | Microdetermination method for measuring minimum inhibitory concentration and minimal bactericidal concentration of cationic antimicrobial peptide | |
| CN201890884U (en) | Blood microorganism culture flask | |
| CN101413872B (en) | Method for rapidly detecting microorganism viable bacteria number | |
| CN105936879B (en) | Bacillus subtilis K13 and its cultural method and application | |
| CN109207554B (en) | Method for rapidly detecting bacteriostatic effect of daily chemical product by using TTC agar culture medium | |
| CN103343157A (en) | Bacterial culture solution for detecting pathogenic bacteria in blood | |
| CN103308360B (en) | The viscosity reduction equipment of toughness clinical memory qu antized table and viscosity reducing process | |
| CN110819691A (en) | Method for identifying and evaluating sterilization effect of disinfectant | |
| CN103157381A (en) | Judgment method of reverse osmosis membrane microbial contamination and application | |
| CN108148888A (en) | Yeast and mold microbial limit detection method | |
| CN111500669A (en) | Yeast qualitative detection method and application | |
| CN201261785Y (en) | Two-phase multi-solid phase blood culture device | |
| CN107287275A (en) | Culture medium, the kit comprising it and its application | |
| CN204939470U (en) | A kind of suspension formula biological indicator | |
| CN103421877B (en) | Sterility test method for antibiotic sustained-release pharmaceutical preparation | |
| CN204897907U (en) | Hinder fungus nature test device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |