CN109845761B - Application of lysozyme two-dimensional nano-film as antibacterial material - Google Patents
Application of lysozyme two-dimensional nano-film as antibacterial material Download PDFInfo
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- CN109845761B CN109845761B CN201910106977.4A CN201910106977A CN109845761B CN 109845761 B CN109845761 B CN 109845761B CN 201910106977 A CN201910106977 A CN 201910106977A CN 109845761 B CN109845761 B CN 109845761B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/34—Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
Abstract
The invention discloses an application of a lysozyme two-dimensional nano-film as an antibacterial material. The lysozyme two-dimensional nano film is used as an antibacterial material, only grows in situ on the surface of the base material in the process of preparing the lysozyme two-dimensional nano film or directly adheres the prepared lysozyme two-dimensional nano film to the surface of the base material, and can grow in situ or adhere to the surfaces of the base materials in various shapes. The lysozyme two-dimensional nano film is used as an antibacterial material, has broad-spectrum and high-efficiency antibacterial performance, can expand the antibacterial spectrum from classical gram-positive bacteria to gram-positive bacteria, gram-negative bacteria and fungi, has good antibacterial efficiency on the three bacteria, can basically reach 90 percent or more in 2 hours, has good adhesion to a substrate to be protected, has no toxicity and adsorbability on cells, has good antifouling property on the cells, and can be used as an ideal antibacterial food packaging material and a medical application program material.
Description
The present application is a divisional application of chinese patent application No. 201510822799.7. The application date of the original application is 11-24.2015, and the invention name is 'application of lysozyme two-dimensional nano-film as an antibacterial material'.
Technical Field
The invention belongs to the technical field of antibacterial materials, and particularly relates to an application of a lysozyme two-dimensional nano-film as an antibacterial material.
Background
In a wide variety of fields including packaging, textile industry, medicine, water purification, health care, etc., it is very important to impart an antibacterial property. Composite materials with antibacterial properties are widely used due to their excellent comprehensive properties, and such composite antibacterial materials can be assembled with different kinds and functions of substances according to different needs, and these characteristics give more and more new functions to the composite materials. However, composite antimicrobial materials also have their own drawbacks. Firstly, monomers used for preparing the composite antibacterial material have different chemical stability relative to surrounding media, so that the composite material has an unstable structure and poor antibacterial effect; secondly, in order to increase the antibacterial property, the conventional method is to add heavy metal antibiotics (such as silver), so that the composite antibacterial material has certain toxicity. Compared with the composite antibacterial material, the traditional composite film antibacterial material overcomes the above shortages, has strong controllability and good orientation, and can obtain a highly ordered composite multilayer film structure, but the harsh synthesis conditions, the complex experimental process and the higher experimental cost often make the composite multilayer film structure incapable of being put into practical industrial application, so that a simple, low-cost and high-repeatability method is very necessary.
Lysozyme is a natural and common antibacterial protein, and the American Food and Drug Administration (FDA) and European Union food additives (E1105) show that it is an effective bacteriostatic and bactericidal active substance, which is considered safe, so that the extraction of antibacterial materials from lysozyme provides an edible and green substance for antibacterial application, and the low-cost commercial source of lysozyme also determines that lysozyme is a key factor for industrial application. Of course, lysozyme, a water-soluble protein molecule, has general disadvantages of protein molecules, such as low mechanical strength, difficulty in forming a continuous thin film structure on a solid surface, poor stability, rapid dissolution in an aqueous solution, and the like. These features limit its application to some extent. In order to solve the above disadvantages, the antibacterial material with high efficiency and durability and the antibacterial material with environmental friendliness, health, no toxicity to cells and corrosion resistance attract more attention, and the current antibacterial film is an ideal surface modified antibacterial material for biological coating commodities, food packaging materials and biomedical equipment (such as guide lines, catheters and surgical knives).
Disclosure of Invention
The invention aims to overcome the defects of the antibacterial material and provide a new application of the lysozyme two-dimensional nano film.
The technical scheme for solving the technical problems is as follows: the lysozyme two-dimensional nano film is applied as an antibacterial material.
The above bacteria are typically gram-positive bacteria (such as Staphylococcus aureus, Diplococcus pneumoniae, and Bacillus tetani), gram-negative bacteria (such as Escherichia coli, Bacillus dysenteriae, and Bacillus typhi), and fungi (such as Candida and Aspergillus taguensis).
The application method of the lysozyme two-dimensional nano film as an antibacterial material comprises the following steps: in the process of preparing the lysozyme two-dimensional nano film, a substrate to be protected is directly contacted with the surface of a solution, so that nanoparticles generated by phase transition of lysozyme are directly self-assembled on the surface of a liquid and a solid through surface interface induction to form a two-dimensional nano film, and grow on the surface of the substrate to be protected in situ to play a role in antibacterial protection, wherein the substrate to be protected is a substrate (such as a silicon wafer, glass, silicon dioxide, polyethylene terephthalate, fibers, hair, polyimide, polyethylene, polypropylene, polycarbonate and the like) with resistance to water; or directly adhering the prepared lysozyme two-dimensional nano film to the surface of a substrate to be protected to play a role in antibacterial protection, wherein the substrate to be protected is a substrate (such as paper, wood, cloth, painting, copper mesh, iron, aluminum, nickel, platinum, gold, copper, silver, electronic devices and the like) sensitive to water. The method disclosed in the invention patent application with the name of "two-dimensional nano-film of biological protein prepared by using lysozyme and preparation method thereof" is specifically referred to as 201510535713.2 (the two-dimensional nano-film of lysozyme of the invention is the two-dimensional nano-film of biological protein therein).
The invention has the following beneficial effects:
1. the lysozyme two-dimensional nano film is used as an antibacterial material, the antibacterial material is natural biomolecule lysozyme, the lysozyme can be widely obtained from the nature, artificial synthesis is not needed, the environmental pollution caused by the traditional antibacterial material during synthesis is avoided, the lysozyme two-dimensional nano film has high transparency (does not influence the appearance color of a base material), low roughness and controllable thickness (more than 30 nm), complex processes such as spin coating, active medium loading and the like are not needed, the operation is simple and convenient, the lysozyme two-dimensional nano film only needs to grow in situ on the surface of the base material in the process of preparing the lysozyme two-dimensional nano film or directly adheres the prepared lysozyme two-dimensional nano film on the surface of the base material, and the lysozyme two-dimensional nano film can grow in situ or adhere to the surfaces of the base materials in various shapes, so that.
2. The lysozyme two-dimensional nano film is used as an antibacterial material, the surface of a base material can be subjected to hydrophilic treatment, the lysozyme two-dimensional nano film is positively charged, the biocompatibility of the hydrophilic surface can be improved in biomedical application, and the positive charges of the surface provide a potential mechanism to kill bacteria.
3. The lysozyme two-dimensional nano film is used as an antibacterial material, has broad-spectrum and high-efficiency antibacterial performance, can expand the antibacterial spectrum from classical gram-positive bacteria to gram-positive bacteria, gram-negative bacteria and fungi, has good antibacterial efficiency on the three bacteria, and can basically reach 90% or more in 2 hours.
4. The lysozyme two-dimensional nano film is used as an antibacterial material, has no toxicity to cells, has a bacterium killing effect and no toxicity to healthy cells, is greatly superior to other antibacterial agents, and can be used as an ideal antibacterial food packaging material and a medical application program material.
5. The lysozyme two-dimensional nano film is used as an antibacterial material, has good adhesion to a substrate to be protected, but has no adsorbability to cells (blood platelets), so that the lysozyme two-dimensional nano film has good antifouling property to the cells.
Drawings
FIG. 1 is a photograph of the lysozyme two-dimensional nano-film grown in situ on the surface of the plastic catheter for medical treatment in example 2.
FIG. 2 is a photograph showing the lysozyme two-dimensional nanofilm adhered to the surface of the surgical knife in example 3.
FIG. 3 is a graph showing the antibacterial efficiency of lysozyme two-dimensional nano-films prepared from different concentrations of lysozyme on Staphylococcus aureus.
FIG. 4 is a graph showing the antibacterial efficiency of lysozyme two-dimensional nano-films prepared from lysozyme at different concentrations against Escherichia coli.
FIG. 5 is a graph showing the antibacterial efficiency of two-dimensional nano-films of lysozyme against Candida prepared from different concentrations of lysozyme.
FIG. 6 is a graph showing the change of antibacterial efficiency of lysozyme two-dimensional nano-film against Staphylococcus aureus and Escherichia coli with time.
FIG. 7 is the laser confocal microscope image of lysozyme two-dimensional nano-film on normal cells.
FIG. 8 is a scanning electron microscope showing the adsorption of normal cells (platelets) by the glass slide (left) and the glass slide (right) of the lysozyme two-dimensional nano-film grown in situ.
Detailed Description
The invention will be further described in detail with reference to the following figures and examples, but the scope of the invention is not limited to these examples.
Example 1
The application of the lysozyme two-dimensional nano film as an antibacterial material comprises the following specific application methods:
0.1433g of tris (2-carboxyethyl) phosphine was added to 10mL of a 10mmol/LpH value 7.4 4-hydroxyethylpiperazine ethanesulfonic acid buffer solution, and the pH was adjusted to 6.4 with NaOH to prepare a 50mmol/L tris (2-carboxyethyl) phosphine 4-hydroxyethylpiperazine ethanesulfonic acid buffer solution; adding 40mg of lysozyme into 10mL of 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution with the value of 10mmol/LpH of 7.4 to prepare 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution of 4mg/mL of lysozyme; uniformly mixing 10mL of 50mmol/L tris (2-carboxyethyl) phosphine 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution with 10mL of 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution of 4mg/mL lysozyme, standing at room temperature for 50 minutes to form a layer of film on the surface of the mixed solution, namely a lysozyme two-dimensional nano film, and transferring the lysozyme two-dimensional nano film to the surface of a glass sheet by taking agarose gel as a medium to play a role in antibacterial protection.
Example 2
The application of the lysozyme two-dimensional nano film as an antibacterial material comprises the following specific application methods:
0.1433g of tris (2-carboxyethyl) phosphine is added into 10mL of a 10mmol/LpH value 7.4 trihydroxymethylaminomethane buffer solution, the pH value is adjusted to 6.4 by NaOH, and 50mmol/L tris (2-carboxyethyl) phosphine trihydroxymethylaminomethane buffer solution is prepared; adding 40mg of lysozyme into 10mL of a trihydroxymethyl aminomethane buffer solution with the value of 10mmol/LpH of 7.4 to prepare a trihydroxymethyl aminomethane buffer solution of 4mg/mL of lysozyme; uniformly mixing 10mL of 50mmol/L tris (2-carboxyethyl) phosphine tris (2-carboxyethyl) aminomethane buffer solution with 10mL of 4mg/mL lysozyme tris (hydroxymethyl) aminomethane buffer solution, inserting the medical plastic catheter into the mixed liquid, standing for 50 minutes at room temperature, and directly forming a layer of self-assembled film (shown in figure 1) on the inner surface and the outer surface of the medical plastic catheter, namely a lysozyme two-dimensional nano film, so as to play a role in antibacterial protection.
Example 3
The application of the lysozyme two-dimensional nano film as an antibacterial material comprises the following specific application methods:
the preparation method of the lysozyme two-dimensional nano-film in the embodiment is the same as that of the embodiment 1, and then the lysozyme two-dimensional nano-film is transferred to the surface of a surgical scalpel by taking agarose gel as a medium (see figure 2) to play a role in antibacterial protection.
Example 4
The application of the lysozyme two-dimensional nano film as an antibacterial material comprises the following specific application methods:
the preparation method of the lysozyme two-dimensional nano-film in the embodiment is the same as that of the embodiment 1, and then the lysozyme two-dimensional nano-film is transferred to the surface of paper by taking agarose gel as a medium, so that the antibacterial protection effect is achieved.
Example 5
The application of the lysozyme two-dimensional nano film as an antibacterial material comprises the following specific application methods:
the preparation method of the lysozyme two-dimensional nano-film in the embodiment is the same as that of the embodiment 1, and then the lysozyme two-dimensional nano-film is transferred to the surface of the bent plastic tube by taking agarose gel as a medium, so that the antibacterial protection effect is achieved.
Example 6
The application of the lysozyme two-dimensional nano film as an antibacterial material comprises the following specific application methods:
in order to prove the beneficial effects of the invention, the inventor carries out a large number of laboratory research experiments, and the specific experimental conditions are as follows:
1. test for antibacterial Properties
0.1433g of tris (2-carboxyethyl) phosphine was added to 10mL of a 10mmol/LpH value 7.4 4-hydroxyethylpiperazine ethanesulfonic acid buffer solution, and the pH was adjusted to 6.4 with NaOH to prepare a 50mmol/L tris (2-carboxyethyl) phosphine 4-hydroxyethylpiperazine ethanesulfonic acid buffer solution; adding lysozyme into 10mL of 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution with the value of 10mmol/LpH of 7.4 to respectively prepare 4-hydroxyethyl piperazine ethanesulfonic acid buffer solutions of lysozyme with the concentration of 2mg/mL, 4mg/mL and 8 mg/mL; taking 10mL of 50mmol/L tris (2-carboxyethyl) phosphine 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution and 10mL of lysozyme 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution with different concentrations, uniformly mixing, standing for 50 minutes at room temperature, and forming a layer of film, namely a lysozyme two-dimensional nano film, on the surface of the mixed solution. Agarose gel is used as a medium to transfer the lysozyme two-dimensional nano-film to the surface of the glass sheet respectively.
Respectively culturing staphylococcus aureus strains and escherichia coli strains in an LB culture medium at 37 ℃ for 16 hours, then inoculating bacterial suspensions into a fresh LB culture medium at 37 ℃ for culture, when OD600 is equal to 0.5, diluting a bacterial culture solution to 5 multiplied by 105CFU/mL by using PBS (phosphate buffer solution) with the pH value of 7.4, then inoculating 10.0 mu L of the diluted bacterial culture solution on a glass sheet adhered with a lysozyme two-dimensional nano film, incubating the glass sheet in a 35mL sterile culture dish at 37 ℃ for 2 hours, then adding 2mL of PBS (phosphate buffer solution) with the pH value of 7.4 into the 35mL sterile culture dish to wash the bacteria, and taking 10.0 mu L to calculate the colony count. The results are shown in FIGS. 3 and 4.
The candida strains are cultured in a culture medium containing a yeast malt culture solution at 28 ℃ for 18 hours, when OD600 is equal to 1.0, the candida culture solution is diluted to 5 multiplied by 105CFU/mL by PBS buffer solution with the pH value of 7.4, then 10.0 mu L of the diluted candida culture solution is inoculated on a glass sheet adhered with a lysozyme two-dimensional nano film, the glass sheet is incubated in a 35mL sterile culture dish at 28 ℃ for 2 hours, then 2mL of PBS buffer solution with the pH value of 7.4 is added to the 35mL sterile culture dish to wash the candida, and 10.0 mu L of the candida strains are taken to calculate the colony count. The results are shown in FIG. 5.
As can be seen from the graphs in FIGS. 3 to 5, the lysozyme two-dimensional nano film has broad-spectrum and high-efficiency antibacterial performance, can generate resistance to gram-positive bacteria, gram-negative bacteria and candida, has good antibacterial efficiency on the three bacteria, and can reach 90 percent or more within 2 hours. And the antibacterial property of the lysozyme two-dimensional nano film prepared with the increase of the concentration of the lysozyme is also improved (all can reach 95 percent or more).
The inventor tests the change of the lysozyme two-dimensional nano-film prepared by 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution of 4mg/mL lysozyme on a glass sheet along with the change of the antibacterial efficiency of staphylococcus aureus and escherichia coli along with the time according to the method, and the result is shown in figure 6. As can be seen from the figure, the killing efficiency of the lysozyme two-dimensional nano film on staphylococcus aureus and escherichia coli can reach more than 90% within 2 hours.
2. Cytotoxicity assays
Fresh human red blood cells (hRBC) were washed three times by centrifugation at 1500 rpm for 15 minutes using tris buffer at pH 7.4 to obtain a concentration of 5.0% (v/v) hRBC suspended in tris buffer, and then the glass slide to which the lysozyme two-dimensional nanofilm of example 1 was adhered was immersed in 2.0mL of tris buffer of the above hRBC, incubated at 37 ℃ for 1 hour at 100 rpm, and thereafter centrifuged at 1500 rpm for 5 minutes. The results are shown in FIG. 7, when observed with a confocal laser microscope. As can be seen, human erythrocytes are not deformed or broken, which shows that the lysozyme two-dimensional nano-film as an antibacterial material has no toxicity to cells.
3. Cell adsorption test
0.1433g of tris (2-carboxyethyl) phosphine is added into 10mL of a 10mmol/LpH value 7.4 trihydroxymethylaminomethane buffer solution, the pH value is adjusted to 4.5 by NaOH, and 50mmol/L tris (2-carboxyethyl) phosphine trihydroxymethylaminomethane buffer solution is prepared; adding 20mg of lysozyme into 10mL of a trihydroxymethyl aminomethane buffer solution with the value of 10mmol/LpH of 7.4 to prepare a trihydroxymethyl aminomethane buffer solution of 2mg/mL of lysozyme; uniformly mixing 10mL of 50mmol/L tris (2-carboxyethyl) phosphine tris (2-carboxyethyl) hydroxymethyl aminomethane buffer solution with 10mL of 2mg/mL lysozyme tris (hydroxymethyl) aminomethane buffer solution, sticking a glass sheet on the surface of the mixed liquid, standing for 50 minutes at room temperature, and directly forming a layer of self-assembled film, namely a lysozyme two-dimensional nano film on the surface of the glass sheet to play a role in antibacterial protection.
Fresh blood from healthy rabbits was centrifuged at 1000 rpm for 15 minutes to obtain platelet plasma (PRP). Immersing the blank glass sheet and the glass sheet adhered with the lysozyme two-dimensional nano-film in PBS (pH value of 7.4) at 37 ℃ for incubation for 1 hour, then injecting 1mLPRP, incubating at 37 ℃ for 30 minutes, and washing the blank glass sheet and the glass sheet adhered with the lysozyme two-dimensional nano-film for 3-5 times by using fresh PBS. Finally, the glass sheet and the glass sheet adhered with the lysozyme two-dimensional nano-film are treated by 2.5 wt% glutaraldehyde aqueous solution at 4 ℃, washed by fresh PBS buffer solution after one night, and dehydrated by ethanol with gradient concentration of 25%, 50%, 75% and 100%. The results are shown in FIG. 8, when observed with a scanning electron microscope. As can be seen, the lysozyme two-dimensional nano-film has no adsorbability on cells (platelets), so that the lysozyme two-dimensional nano-film has good antifouling property on the cells.
Claims (6)
1. An antibacterial material lysozyme two-dimensional nano-film is characterized in that: the preparation method of the lysozyme two-dimensional nano film comprises the following steps:
0.1433g of tris (2-carboxyethyl) phosphine is added into 10mL of 10 mmol/L4-hydroxyethylpiperazine ethanesulfonic acid buffer solution with the pH value of 7.4, the pH value is adjusted to 6.4 by NaOH, and 50mmol/L tris (2-carboxyethyl) phosphine 4-hydroxyethylpiperazine ethanesulfonic acid buffer solution is prepared; adding 40mg of lysozyme into 10mL of 10 mmol/L4-hydroxyethyl piperazine ethanesulfonic acid buffer solution with the pH value of 7.4 to prepare 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution of 4mg/mL lysozyme; uniformly mixing 10mL of a 50mmol/L tris (2-carboxyethyl) phosphine 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution with 10mL of a 4mg/mL lysozyme 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution, standing at room temperature for 50 minutes to form a lysozyme two-dimensional nano film on the surface of the mixed solution, and transferring the lysozyme two-dimensional nano film to the surface of a glass sheet by taking agarose gel as a medium.
2. An antibacterial material lysozyme two-dimensional nano-film is characterized in that: the preparation method of the lysozyme two-dimensional nano film comprises the following steps:
0.1433g of tris (2-carboxyethyl) phosphine is added into 10mL of 10mmol/L tris (2-carboxyethyl) phosphine buffer solution with pH value of 7.4, and the pH value is adjusted to 6.4 by NaOH to prepare 50mmol/L tris (2-carboxyethyl) phosphine tris (hydroxymethyl) aminomethane buffer solution; adding 40mg of lysozyme into 10mL of 10mmol/L trihydroxymethyl aminomethane buffer solution with the pH value of 7.4 to prepare 4mg/mL trihydroxymethyl aminomethane buffer solution of lysozyme; uniformly mixing 10mL of 50mmol/L tris (2-carboxyethyl) phosphine tris (2-carboxyethyl) hydroxymethyl aminomethane buffer solution with 10mL of 4mg/mL lysozyme tris (hydroxymethyl aminomethane buffer solution), inserting the medical plastic catheter into the mixed liquid, standing for 50 minutes at room temperature, and directly forming a layer of self-assembled film, namely the lysozyme two-dimensional nano film, on the inner surface and the outer surface of the medical plastic catheter.
3. The method for using the lysozyme two-dimensional nano film as an antibacterial material in claim 1 or 2 is characterized in that: directly contacting a substrate to be protected with the surface of a solution in the process of preparing the lysozyme two-dimensional nano film, so that nanoparticles generated by phase transition of lysozyme are directly self-assembled on the surface of a liquid and a solid through surface interface induction to form the two-dimensional nano film, and grow on the surface of the substrate to be protected in situ to play a role in antibacterial protection, wherein the substrate to be protected is fiber, hair, polyimide, polyethylene, polypropylene or polycarbonate;
wherein the solution is a 4-hydroxyethylpiperazine ethanesulfonic acid buffer solution containing tris (2-carboxyethyl) phosphine of lysozyme, or a tris (2-carboxyethyl) phosphine tris (2-carboxyethyl) methane buffer solution containing lysozyme;
the lysozyme two-dimensional nano film only contains lysozyme as an antibacterial component;
the preparation process does not need spin coating or active medium loading;
in the using process, the lysozyme two-dimensional nano film only needs to be grown on the surface of the base material in situ in the process of preparing the lysozyme two-dimensional nano film or directly adheres the prepared lysozyme two-dimensional nano film on the surface of the base material, and the lysozyme two-dimensional nano film is grown in situ or adheres to the surfaces of the base materials in various shapes, so that large-area preparation is realized.
4. The use method of the two-dimensional nano film of the antibacterial material lysozyme as the antibacterial material according to claim 3 is characterized in that: the prepared lysozyme two-dimensional nano film is directly adhered to the surface of a substrate to be protected to play a role in antibacterial protection, wherein the substrate to be protected is a substrate sensitive to water.
5. The use method of the two-dimensional nano film of the antibacterial material lysozyme, which is taken as the antibacterial material, is characterized in that: the base material sensitive to water is any one of paper, wood, cloth, painting, copper mesh, iron, aluminum, nickel, platinum, silver and electronic devices.
6. The use of the two-dimensional nanofilm of lysozyme described in claim 1 or 2 as an antibacterial material, said bacteria being gram-positive bacteria, gram-negative bacteria and fungi, wherein the gram-positive bacteria are staphylococcus aureus, diplococcus pneumoniae or tetanus bacillus, the gram-negative bacteria are escherichia coli, dysentery bacillus or typhoid bacillus, and the fungi are candida or taggantiacus.
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| CN108310447A (en) * | 2018-03-22 | 2018-07-24 | 陕西师范大学 | A kind of bacteriolyze enzyme modification hospital gauze |
| CN108854599B (en) * | 2018-05-14 | 2020-10-16 | 陕西师范大学 | Dialysis membrane based on cross-linked lysozyme and application thereof |
| CN113908336B (en) * | 2021-09-29 | 2022-11-04 | 重庆医科大学附属口腔医院 | Lysozyme phase transition membrane modified cellulose scaffold and preparation method and application thereof |
| CN117442702A (en) * | 2022-01-26 | 2024-01-26 | 陕西师范大学 | Antibacterial liquid with controllable-size protein-based nanoparticles |
| CN115444031A (en) * | 2022-09-01 | 2022-12-09 | 陕西师范大学 | Protein-based fruit antibacterial fresh-keeping edible coating and preparation method and application thereof |
| CN115365494B (en) * | 2022-09-13 | 2023-07-07 | 嘉兴学院 | Preparation method of silver-coated copper powder and application of silver-coated copper powder in conductive paste |
| CN116196905B (en) * | 2023-04-03 | 2024-01-30 | 四川省原子能研究院 | Endotoxin adsorption film and preparation method thereof |
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| CN102505041A (en) * | 2011-10-08 | 2012-06-20 | 杨鹏 | Preparation method of biological chip |
| CN105153443A (en) * | 2015-08-27 | 2015-12-16 | 陕西师范大学 | Biological protein two-dimensional nano-film prepared by using lysozyme and preparation method of biological protein two-dimensional nano-film |
| CN105204291A (en) * | 2015-10-29 | 2015-12-30 | 陕西师范大学 | Application of two-dimensional lysozyme nano-film as photoresist |
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| CN105475359B (en) | 2020-06-26 |
| CN105475359A (en) | 2016-04-13 |
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