CN116196905B - Endotoxin adsorption film and preparation method thereof - Google Patents
Endotoxin adsorption film and preparation method thereof Download PDFInfo
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- CN116196905B CN116196905B CN202310348005.2A CN202310348005A CN116196905B CN 116196905 B CN116196905 B CN 116196905B CN 202310348005 A CN202310348005 A CN 202310348005A CN 116196905 B CN116196905 B CN 116196905B
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- 239000002158 endotoxin Substances 0.000 title claims abstract description 58
- 238000001179 sorption measurement Methods 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title abstract description 11
- 102000016943 Muramidase Human genes 0.000 claims abstract description 58
- 108010014251 Muramidase Proteins 0.000 claims abstract description 58
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims abstract description 58
- 229960000274 lysozyme Drugs 0.000 claims abstract description 58
- 235000010335 lysozyme Nutrition 0.000 claims abstract description 58
- 239000004325 lysozyme Substances 0.000 claims abstract description 58
- 239000004695 Polyether sulfone Substances 0.000 claims abstract description 31
- 229920006393 polyether sulfone Polymers 0.000 claims abstract description 31
- 239000000758 substrate Substances 0.000 claims abstract description 27
- 150000007524 organic acids Chemical class 0.000 claims abstract description 21
- 229920006254 polymer film Polymers 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 230000004048 modification Effects 0.000 claims abstract description 9
- 238000012986 modification Methods 0.000 claims abstract description 9
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 5
- 230000005251 gamma ray Effects 0.000 claims abstract description 4
- 230000001681 protective effect Effects 0.000 claims abstract description 3
- 230000005855 radiation Effects 0.000 claims description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 239000006228 supernatant Substances 0.000 claims description 11
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Substances CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 claims description 9
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 claims description 9
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 claims description 9
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 7
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 238000004090 dissolution Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- NGSWKAQJJWESNS-UHFFFAOYSA-N 4-coumaric acid Chemical compound OC(=O)C=CC1=CC=C(O)C=C1 NGSWKAQJJWESNS-UHFFFAOYSA-N 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims description 4
- 229960004889 salicylic acid Drugs 0.000 claims description 4
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 claims description 3
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical group COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 claims description 3
- 229940114124 ferulic acid Drugs 0.000 claims description 3
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 claims description 3
- 235000001785 ferulic acid Nutrition 0.000 claims description 3
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 claims description 3
- NGSWKAQJJWESNS-ZZXKWVIFSA-M 4-Hydroxycinnamate Natural products OC1=CC=C(\C=C\C([O-])=O)C=C1 NGSWKAQJJWESNS-ZZXKWVIFSA-M 0.000 claims description 2
- DFYRUELUNQRZTB-UHFFFAOYSA-N Acetovanillone Natural products COC1=CC(C(C)=O)=CC=C1O DFYRUELUNQRZTB-UHFFFAOYSA-N 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 239000012528 membrane Substances 0.000 abstract description 13
- 239000008280 blood Substances 0.000 abstract description 8
- 210000004369 blood Anatomy 0.000 abstract description 8
- 229920002492 poly(sulfone) Polymers 0.000 abstract description 7
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 33
- 238000006243 chemical reaction Methods 0.000 description 13
- 239000011259 mixed solution Substances 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 230000000052 comparative effect Effects 0.000 description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 239000008367 deionised water Substances 0.000 description 7
- 229910021641 deionized water Inorganic materials 0.000 description 7
- 238000011068 loading method Methods 0.000 description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 239000003446 ligand Substances 0.000 description 5
- 238000001291 vacuum drying Methods 0.000 description 5
- 238000011049 filling Methods 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- CTENFNNZBMHDDG-UHFFFAOYSA-N Dopamine hydrochloride Chemical compound Cl.NCCC1=CC=C(O)C(O)=C1 CTENFNNZBMHDDG-UHFFFAOYSA-N 0.000 description 3
- 239000004698 Polyethylene Substances 0.000 description 3
- 108010093965 Polymyxin B Proteins 0.000 description 3
- 229960001149 dopamine hydrochloride Drugs 0.000 description 3
- 238000011013 endotoxin removal Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229920001690 polydopamine Polymers 0.000 description 3
- 229920000573 polyethylene Polymers 0.000 description 3
- 229920000024 polymyxin B Polymers 0.000 description 3
- 229960005266 polymyxin b Drugs 0.000 description 3
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000003463 adsorbent Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 2
- 229960003964 deoxycholic acid Drugs 0.000 description 2
- KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 238000002329 infrared spectrum Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 208000037487 Endotoxemia Diseases 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- 208000034486 Multi-organ failure Diseases 0.000 description 1
- 208000010718 Multiple Organ Failure Diseases 0.000 description 1
- 206010029155 Nephropathy toxic Diseases 0.000 description 1
- 206010029350 Neurotoxicity Diseases 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 206010040070 Septic Shock Diseases 0.000 description 1
- 206010044221 Toxic encephalopathy Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000002843 carboxylic acid group Chemical group 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000009190 disseminated intravascular coagulation Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000009775 high-speed stirring Methods 0.000 description 1
- 229960001340 histamine Drugs 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- -1 i.e. Substances 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 238000012844 infrared spectroscopy analysis Methods 0.000 description 1
- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 208000029744 multiple organ dysfunction syndrome Diseases 0.000 description 1
- 230000007694 nephrotoxicity Effects 0.000 description 1
- 231100000417 nephrotoxicity Toxicity 0.000 description 1
- 230000007135 neurotoxicity Effects 0.000 description 1
- 231100000228 neurotoxicity Toxicity 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920005597 polymer membrane Polymers 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002510 pyrogen Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000036303 septic shock Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/262—Synthetic macromolecular compounds obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
- B01J20/28033—Membrane, sheet, cloth, pad, lamellar or mat
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses an endotoxin adsorption film and a preparation method thereof, belonging to the technical field of endotoxin adsorption. The endotoxin adsorption film comprises a high polymer film substrate and lysozyme grafted on the substrate, wherein the high polymer film substrate is a polyether sulfone film or a polysulfone film. During preparation, the lysozyme is modified by organic acid with a hydrophobic structure, then the high polymer film substrate is immersed in the lysozyme modification liquid, gamma-ray irradiation is carried out in a protective gas atmosphere, so as to obtain a lysozyme-high polymer film substrate mixed liquid, and then the mixture liquid is washed and dried, so that the endotoxin adsorption film is obtained. The endotoxin adsorption film prepared by the invention is loaded with lysozyme; lysozyme can specifically adsorb endotoxin, so that endotoxin toxicity is reduced; the lysozyme loaded polyethersulfone/polysulfone has good blood compatibility and can be prepared into a specific affinity adsorption membrane.
Description
Technical Field
The invention belongs to the technical field of endotoxin adsorption, and particularly relates to an endotoxin adsorption film and a preparation method thereof.
Background
Endotoxin, also known as lipopolysaccharide, is a characteristic component of gram-negative bacteria and is a highly toxic inflammatory and pyrogenic substance. Endotoxemia can occur in a variety of diseases of the multisystem, often resulting in sepsis, fatal septic shock, multiple organ failure, disseminated intravascular coagulation, and the like. The complexity of endotoxin structure, complexity of blood environment and low abundance of endotoxin in blood lead to very difficult separation of endotoxin in blood. The existing endotoxin removal mainly depends on the electrostatic action and the hydrophobic action of the material, but the application of the material is very limited due to the non-specific adsorption, so that the key of endotoxin adsorption in blood is to find a ligand capable of specifically binding to endotoxin and a proper affinity matrix, and the ligand is fixed on the matrix by a proper method to obtain the affinity adsorbent with the adsorption action on the endotoxin.
Common ligands for preparing endotoxin affinity adsorbents include: immune affinity ligand, polymyxin B (polymyxin B), histamine and histidine (His), deoxycholic acid (DOC), polymeric cation, amine, amino acid, etc. Polymyxin B is the most used ligand with good endotoxin removal effect, but its own neurotoxicity and nephrotoxicity are the main reasons limiting its wide application, and are expensive, making the treatment cost high. Detoxification of agents with blood is a new direction of development of affinity membrane technology. Compared with the traditional active carbon and synthetic resin carrier, the polymer membrane material has excellent use characteristics, such as smooth diffusion path and low operation pressure, and is beneficial to affinity adsorption of poison molecules. In addition, the good blood compatibility of various membrane materials such as polysulfone, polyethersulfone and the like is also recognized, and a suitable carrier can be provided for developing specific affinity adsorption membranes with more different purposes.
Disclosure of Invention
Aiming at the prior art, the invention provides an endotoxin adsorption film and a preparation method thereof, which are used for solving the technical problems that endotoxin removal is difficult and the loading capacity of lysozyme on a substrate is low in the prior art.
In order to achieve the above purpose, the technical scheme adopted by the invention is to provide an endotoxin adsorption film, which comprises a high polymer film substrate and lysozyme grafted on the substrate.
On the basis of the technical scheme, the invention can be improved as follows.
Further, the high polymer film substrate is a polyethersulfone film or a polysulfone film.
The invention also discloses a preparation method of the endotoxin adsorption film, which comprises the following steps:
s1: modifying lysozyme by using organic acid with a hydrophobic structure to obtain lysozyme modification solution;
s2: immersing a high polymer film substrate in lysozyme modified liquid, and carrying out gamma-ray irradiation in a protective gas atmosphere, wherein the radiation dose is 2-12 kGy, and the radiation dose rate is 50-70 Gy/h, so as to obtain a lysozyme-high polymer film substrate mixed liquid; and washing and drying to obtain the endotoxin adsorption film.
Further, the lysozyme modification solution is prepared through the following steps:
SS1: mixing organic acid with alkali liquor, regulating the pH value of the mixture to 7-8, adding 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide into the obtained mixed liquor, and standing for 35-45 min at room temperature after complete dissolution;
SS2: adding lysozyme into the mixture after standing, stirring for 20-25 h at 30-40 ℃, centrifuging, and collecting the supernatant to obtain the lysozyme modified liquid.
Further, the organic acid with a hydrophobic structure is ferulic acid, p-coumaric acid or salicylic acid, and the alkali liquor is sodium hydroxide solution with the concentration of 5 mol/L.
Further, the mass ratio of the 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride to the N-hydroxysuccinimide to the organic acid is 10-15:5-10:1-5.
Further, the mass ratio of the 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride and the N-hydroxysuccinimide to the organic acid is 12:8:3.
Further, the mass ratio of lysozyme to organic acid added by SS2 was 2:1.
Further, the gamma rays are 60 Co-gamma rays.
Further, the drying temperature in S2 was 35 ℃.
The beneficial effects of the invention are as follows:
1. according to the invention, the lysozyme is modified by using the organic acid with a hydrophobic structure, on one hand, the carboxylic acid group in the organic acid can modify the terminal residue of the lysozyme without affecting the active position; on the other hand, as the active site of the lysozyme is separated from water by the hydrophobic fatty chain, hydroxyl free radicals in the water attack the terminal residues of the lysozyme during radiation are reduced, and the damage of the secondary structure of the lysozyme is reduced.
2. According to the invention, lysozyme on the endotoxin adsorption film is grafted in an irradiation manner, the grafting can be realized by a gamma ray irradiation grafting method without additional additive components, and meanwhile, lysozyme grafted on a polyethersulfone/polysulfone molecular chain cannot fall off along with the endotoxin adsorption process; meanwhile, the grafting rate of the product can be well controlled by changing the irradiation absorption dose and dose rate and adding some anti-grafting agents, the irradiation grafting reaction process is more convenient, and the application value is higher.
3. The endotoxin adsorption film prepared by the invention is loaded with lysozyme, and the basic structure of endotoxin is formed by three parts of covalent bonds, namely O-specific antigen polysaccharide, core polysaccharide and lipoid A, wherein lipoid A is the main toxic component of biological activity of endotoxin; lysozyme can specifically adsorb endotoxin, so that endotoxin toxicity is reduced; the lysozyme loaded polyethersulfone/polysulfone has good blood compatibility and can be prepared into a specific affinity adsorption membrane.
Drawings
FIG. 1 is an infrared spectrum of different endotoxin-adsorbing films.
Detailed Description
The following describes the present invention in detail with reference to examples.
Example 1
An endotoxin adsorption film comprises a polyethersulfone film substrate and lysozyme grafted on the polyethersulfone film.
The endotoxin adsorption film in this example was prepared by the following steps:
s1: dissolving 30mg of ferulic acid in 5mL of sodium hydroxide solution with the concentration of 3mol/L, and regulating the pH of the mixed solution to 7.5 by using 5mol/L hydrochloric acid to obtain an organic acid solution; then adding 120mg of 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride and 80mg of N-hydroxysuccinimide into the organic acid solution respectively, standing for 40min at room temperature of 25 ℃ after complete dissolution; and adding 60mg of lysozyme into the mixed solution after standing, stirring for 24 hours in a water bath at 35 ℃, centrifuging (4000 Xg, 15 min) to remove insoluble parts in the system after the reaction is finished, collecting supernatant, and placing the supernatant into a solution bottle, wherein the collected supernatant is the lysozyme modified solution.
S2: the polyethersulfone film is placed in a solution bottle, and the lysozyme modification solution can completely submerge the film. Placing the mixed solution in a radiation reaction device, filling nitrogen, and sealing the radiation reaction device; normal temperature and pressure channel 60 Co-gamma rays are radiated for 10kGy, the radiation dosage rate is 60Gy/h, and lysozyme-polyethersulfone copolymer film mixed solution is obtained; washing the film with deionized water, filtering, and vacuum drying at 35 deg.c to constant weight to obtain lysozyme-polyethersulfone copolymer film, i.e. endotoxin adsorbing film.
Example 2
An endotoxin adsorption film comprises a polyethersulfone film substrate and lysozyme grafted on the polyethersulfone film.
The endotoxin adsorption film in this example was prepared by the following steps:
s1: 10mg of salicylic acid is dissolved in 5mL of sodium hydroxide solution with the concentration of 1mol/L, and the pH value of the mixed solution is regulated to 7 by 1mol/L of hydrochloric acid, so as to obtain an organic acid solution; then adding 100mg of 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride and 50mg of N-hydroxysuccinimide into the organic acid solution respectively, standing for 35min at room temperature of 25 ℃ after complete dissolution; and adding 20mg of lysozyme into the mixed solution after standing, stirring for 25 hours in a water bath at 30 ℃, centrifuging (4000 Xg, 15 min) to remove insoluble parts in the system after the reaction is finished, collecting supernatant, and placing the supernatant into a solution bottle, wherein the collected supernatant is the lysozyme modified solution.
S2: the polyethersulfone film is placed in a solution bottle, and the lysozyme modification solution can completely submerge the film. Placing the mixed solution in a radiation reaction device, filling nitrogen, and sealing the radiation reaction device; normal temperature and pressure channel 60 Co-gamma rays are radiated for 2kGy, the radiation dosage rate is 60Gy/h, and lysozyme-polyethersulfone copolymer film mixed solution is obtained; washing the film with deionized water, filtering, and vacuum drying at 35 deg.c to constant weight to obtain lysozyme-polyethersulfone copolymer film, i.e. endotoxin adsorbing film.
Example 3
An endotoxin adsorption film comprises a polyethersulfone film substrate and lysozyme grafted on the polyethersulfone film.
The endotoxin adsorption film in this example was prepared by the following steps:
s1: dissolving 50mg of salicylic acid in 5mL of sodium hydroxide solution with the concentration of 5mol/L, and regulating the pH value of the mixed solution to 8 by using 1mol/L of hydrochloric acid to obtain an organic acid solution; then respectively adding 150mg of 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride and 100mg of N-hydroxysuccinimide into the organic acid solution, standing for 45min at room temperature of 25 ℃ after complete dissolution; and adding 100mg of lysozyme into the mixed solution after standing, stirring for 20 hours in a water bath at 40 ℃, centrifuging (4000 Xg, 15 min) to remove insoluble parts in the system after the reaction is finished, collecting supernatant, and placing the supernatant into a solution bottle, wherein the collected supernatant is the lysozyme modified solution.
S2: the polyethersulfone film is placed in a solution bottle, and the lysozyme modification solution can completely submerge the film. Placing the mixed solution in a radiation reaction device, filling nitrogen, and sealing the radiation reaction device; normal temperature and pressure channel 60 Co-gamma rays are radiated for 12kGy, the radiation dosage rate is 60Gy/h, and lysozyme-polyethersulfone copolymer film mixed solution is obtained; washing the film with deionized water, filtering, and vacuum drying at 35deg.C to constant weight to obtain lysozyme-polyEther sulfone copolymer membranes, i.e., endotoxin adsorbing membranes.
Comparative example 1
An endotoxin adsorption film was obtained by replacing the substrate with a polyethersulfone film as in example 1 (preparation method was referred to "preparation of polyethersulfone graft polyethylene glycol copolymer by click", meng Jianjiang, etc.), and the other steps were the same as in example 1.
Comparative example 2
An endotoxin adsorption membrane was obtained by replacing the substrate with a polyethersulfone membrane and then by replacing the substrate with a polyethersulfone-g-polyethylene glycol-polydopamine membrane in comparison with example 1, and the other steps were the same as in example 1. The preparation method of the polyethersulfone-g-polyethylene glycol-polydopamine film comprises the following steps:
accurately measuring dopamine hydrochloride powder, dissolving the dopamine hydrochloride powder in deionized water under high-speed stirring to prepare 2g/L aqueous solution, and then regulating the pH of the solution to 8.5 by using Tris (Tris), wherein the prepared solution presents light brown; putting the polyethersulfone-g-polyethylene glycol film substrate into a newly prepared dopamine hydrochloride solution bottle, wherein the solution can completely submerge the film; placing the film into a shaking table without cover to react for 24 hours at 37 ℃ and 100r/min, taking out the film after the reaction is finished, washing the film with a large amount of deionized water to remove redundant agglomerated particles, soaking the film in the deionized water for 2 hours, and vacuum drying the film to constant weight at 35 ℃ to obtain the dark brown polyethersulfone-g-polyethylene glycol-polydopamine film.
Comparative example 3
An endotoxin adsorption film comprises a polyethersulfone film substrate and lysozyme grafted on the polyethersulfone film.
The endotoxin adsorption film in the present comparative example was produced by the following steps:
s1: 60mg of lysozyme was dissolved in 5mL of water to prepare a lysozyme solution, and the lysozyme solution was placed in a solution bottle.
S2: the polyethersulfone membrane is placed in a solution bottle, and the lysozyme solution can completely submerge the membrane. Placing the mixed solution in a radiation reaction device, filling nitrogen, and sealing the radiation reaction device; normal temperature and pressure channel 60 Co-gamma ray radiation of 10kGy with a radiation dose rate of 60Gy/hTo lysozyme-polyethersulfone copolymer film mixed liquor; washing the film with deionized water, filtering, and vacuum drying at 35 deg.c to constant weight to obtain lysozyme-polyethersulfone copolymer film, i.e. endotoxin adsorbing film.
Analysis of results
The endotoxin adsorption films prepared in example 1 (PES-Lys), comparative example 1 (PES-g-PEG-Lys) and comparative example 2 (PES-g-PEG-PDA-Lys) were respectively subjected to infrared spectroscopic analysis, and the results are shown in FIG. 1. As can be seen from FIG. 1, there are characteristic peaks of lysozyme in the infrared spectrum, indicating successful loading of lysozyme onto the film substrate.
The loading amounts of lysozyme on the endotoxin adsorption films prepared in example 1, comparative example 2 and comparative example 3 were also examined, and the results are shown in Table 1.
TABLE 1 lysozyme loading (U/mL) on different endotoxin adsorption membranes
| Example 1 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| Lysozyme loading | 3388.0 | 1633.5 | 1389.4 | 1894.3 |
As can be seen from table 1, the substrate type and the treatment mode of the lysozyme have significant influence on the loading amount of the lysozyme, and the substrate and the preparation process in the application can significantly improve the loading amount of the lysozyme on the polyethersulfone/polysulfone film, so that the prepared endotoxin adsorption film can exert more excellent endotoxin adsorption effect.
While specific embodiments of the invention have been described in detail in connection with the examples, it should not be construed as limiting the scope of protection of the patent. Various modifications and variations which may be made by those skilled in the art without the creative effort are within the scope of the patent described in the claims.
Claims (7)
1. An endotoxin-adsorbing film, which is characterized in that: comprises a high polymer film substrate and lysozyme grafted on the substrate; the high polymer film substrate is a polyethersulfone film; the endotoxin adsorption film is prepared through the following steps:
s1: modifying lysozyme by using organic acid with a hydrophobic structure to obtain lysozyme modification solution; the lysozyme modification solution is prepared through the following steps:
SS1: mixing organic acid with alkali liquor, regulating the pH value of the mixture to 7-8, adding 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide into the obtained mixed liquor, and standing for 35-45 min at room temperature after complete dissolution;
SS2: adding lysozyme into the mixture after standing, stirring for 20-25 hours at 30-40 ℃, centrifuging, and collecting the supernatant to obtain lysozyme modified liquid;
s2: immersing a high polymer film substrate in lysozyme modified liquid, and carrying out gamma-ray irradiation in a protective gas atmosphere, wherein the radiation dose is 2-12 kGy, and the radiation dose rate is 50-70 Gy/h, so as to obtain a lysozyme-high polymer film substrate mixed liquid; and washing and drying to obtain the endotoxin adsorption film.
2. The endotoxin adsorption film according to claim 1, wherein the organic acid with a hydrophobic structure is ferulic acid, p-coumaric acid or salicylic acid, and the alkali solution is sodium hydroxide solution with a concentration of 5 mol/L.
3. An endotoxin adsorption film according to claim 1, wherein: the mass ratio of the 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride to the N-hydroxysuccinimide to the organic acid is 10-15:5-10:1-5.
4. An endotoxin adsorption film as claimed in claim 3, wherein: the mass ratio of the 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride to the N-hydroxysuccinimide to the organic acid is 12:8:3.
5. An endotoxin adsorption film according to claim 1, wherein: the mass ratio of the lysozyme to the organic acid added by the SS2 is 2:1.
6. An endotoxin adsorption film according to claim 1, wherein: the gamma rays are 60 Co-gamma rays.
7. An endotoxin adsorption film according to claim 1, wherein: the drying temperature in S2 was 35 ℃.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102361689A (en) * | 2009-01-22 | 2012-02-22 | 弗雷森纽斯医疗护理德国有限责任公司 | Sorbent for endotoxins |
| JP2016049527A (en) * | 2014-09-02 | 2016-04-11 | 国立大学法人 熊本大学 | Endotoxin adsorbent |
| CN105475359A (en) * | 2015-11-24 | 2016-04-13 | 陕西师范大学 | Application of two-dimensional lysozyme nanometer film as antibacterial material |
| CN107486176A (en) * | 2017-09-11 | 2017-12-19 | 广州康盛生物科技有限公司 | A kind of sorbing material for blood purification and preparation method thereof |
| CN108714416A (en) * | 2018-05-29 | 2018-10-30 | 成都新柯力化工科技有限公司 | A kind of broad spectrum activity depth blood purification sorbing material and preparation method for medical treatment |
| CN114669282A (en) * | 2022-04-07 | 2022-06-28 | 浙江大学 | Endotoxin adsorbent and preparation method and application thereof |
-
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102361689A (en) * | 2009-01-22 | 2012-02-22 | 弗雷森纽斯医疗护理德国有限责任公司 | Sorbent for endotoxins |
| JP2016049527A (en) * | 2014-09-02 | 2016-04-11 | 国立大学法人 熊本大学 | Endotoxin adsorbent |
| CN105475359A (en) * | 2015-11-24 | 2016-04-13 | 陕西师范大学 | Application of two-dimensional lysozyme nanometer film as antibacterial material |
| CN107486176A (en) * | 2017-09-11 | 2017-12-19 | 广州康盛生物科技有限公司 | A kind of sorbing material for blood purification and preparation method thereof |
| CN108714416A (en) * | 2018-05-29 | 2018-10-30 | 成都新柯力化工科技有限公司 | A kind of broad spectrum activity depth blood purification sorbing material and preparation method for medical treatment |
| CN114669282A (en) * | 2022-04-07 | 2022-06-28 | 浙江大学 | Endotoxin adsorbent and preparation method and application thereof |
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