CN109845761A - Application of the lysozyme two-dimensional nano-film as anti-biotic material - Google Patents
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- CN109845761A CN109845761A CN201910106977.4A CN201910106977A CN109845761A CN 109845761 A CN109845761 A CN 109845761A CN 201910106977 A CN201910106977 A CN 201910106977A CN 109845761 A CN109845761 A CN 109845761A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/34—Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
Abstract
A kind of application the invention discloses lysozyme two-dimensional nano-film as anti-biotic material.The present invention is using lysozyme two-dimensional nano-film as anti-biotic material, only growth in situ in substrate surface or directly by the lysozyme two-dimensional nano-film prepared is adhered to substrate surface during preparing lysozyme two-dimensional nano-film, and with growth in situ or can be adhered to substrate surface of various shapes.The present invention is using lysozyme two-dimensional nano-film as anti-biotic material, with wide spectrum and efficient anti-microbial property, antimicrobial spectrum can be expanded to gram-positive bacteria from classic gram-positive bacteria one kind bacterium, Gram-negative bacteria and fungi three classes bacterium, and good antimicrobial efficiency is presented to these three types of bacterium, antimicrobial efficiency was all up substantially at 2 hours to 90% or more, it has good adhesiveness to substrate to be protected, to cytotoxic and adsorptivity, therefore there is good soil resistance to cell, it is alternatively arranged as a kind of ideal antibacterial food packaging material and medical applications material.
Description
The application is Chinese patent application the 201510822799.7th divisional application.The applying date of original application is 2015
It is on November 24, in, entitled " application of the lysozyme two-dimensional nano-film as anti-biotic material ".
Technical field
The invention belongs to anti-biotic material technical fields, and in particular to a kind of lysozyme two-dimensional nano-film is as anti-biotic material
Application.
Background technique
Include packaging, textile industry, medicine and pharmacology, Water warfare and health care etc. in various fields, assigns one
Anti-microbial property is very important.Composite material with antibiotic property since its excellent comprehensive performance is widely used, this
Kind composite antibacterial material can assemble variety classes and the substance of function according to different, these characteristics impart multiple
The function that condensation material is more, updates.However, composite antibacterial material also has the shortcomings that its own.First, it is compound anti-for preparing
The monomer of bacterium material has different chemical stabilities with respect to surrounding medium, and it is unstable to will lead to composite structure, antibacterial effect
Fruit is bad；Second, in order to increase its antibiotic property, traditional method is that heavy metal antibiotic (such as silver), such composite antibacterial is added
Material inherently has certain toxicity.For composite antibacterial material, traditional laminated film anti-biotic material overcome with
Shortage above, this thin-film material controllability is strong, favorable orientation, can also obtain the composite multi-layer membrane structure of high-sequential,
But harsh synthesis condition, complicated experimentation and higher experimental cost usually makes it cannot be introduced into actual industry
In, then, the method for simple, inexpensive and higher repeatability is very required.
Lysozyme is a kind of natural, common antibacterial protein, food and drug administration (FDA) and European Union's food
Additive (E1105) shows that it is a kind of effective antibacterial, sterilization bactericidal activity object, it is considered to be it is safe, therefore, from molten
It is just that antibacterial applications provide for a kind of edible and green substance that anti-biotic material is extracted in bacterium enzyme, and the commercialization of lysozyme comes
Source inexpensively also determines its key factor as industrial application.Certainly, as a kind of water-soluble protein molecule, lysozyme
Have the shortcomings that protein molecule is universal, such as mechanical strength is low, be difficult to form continuous membrane structure in the surface of solids, stablizes
Property is bad, can dissolve rapidly in aqueous solution.These features limit its application to a certain extent.Solve above kind
Kind disadvantage obtains the anti-biotic material and environment friendly, the healthy antibacterial to cytotoxic, corrosion resistance of high-efficient and lasting
Material more causes the concern of people, at present antibacterial film to biological coating commodity, packaging material for food and biomedical devices (such as
Guide line, conduit and surgery knife) it is an ideal the modified anti-biotic material in surface.
Summary of the invention
It is that lysozyme two-dimensional nano is thin technical problem to be solved by the present invention lies in above-mentioned anti-biotic material is overcome
Film provides a kind of new purposes.
Solving technical solution used by above-mentioned technical problem is: lysozyme two-dimensional nano-film is answered as anti-biotic material
With.
Above-mentioned bacterium is typical gram-positive bacteria (such as staphylococcus aureus, Diplococcus pneumopniae, clostridium tetani
Deng), Gram-negative bacteria (such as Escherichia coli, shigella dysenteriae, typhoid bacillus) and fungi (such as candida albicans, fugue bacterium).
Application method of the above-mentioned lysozyme two-dimensional nano-film as anti-biotic material are as follows: lysozyme two-dimensional nano is thin preparing
Directly substrate to be protected is contacted with solution surface during film, the nano particle for generating lysozyme phase transition passes through table
Interface induced directly to consolidate surface self-organization formation two-dimensional nano-film in liquid, growth in situ rises anti-in substrate surface to be protected
The effect of bacterium protection, wherein the substrate to be protected is substrate (such as silicon wafer, glass, the dioxy for having resistance to water
SiClx, polyethylene terephthalate, fiber, hairline, polyimides, polyethylene, polypropylene, polycarbonate etc.)；Or
The lysozyme two-dimensional nano-film prepared is attached directly to substrate surface to be protected, plays antimicrobial protection, wherein
The substrate to be protected be to water have sensibility substrate (such as paper, timber, cloth, drawing, copper mesh, iron, aluminium, nickel,
Platinum, gold, copper, silver, electronic device etc.).With specific reference to application No. is 201510535713.2, entitled " utilize lysozyme
Method disclosed in the application for a patent for invention of bioprotein two-dimensional nano-film of preparation and preparation method thereof " is (of the invention
Lysozyme two-dimensional nano-film is bioprotein two-dimensional nano-film therein).
Beneficial effects of the present invention are as follows:
1, for the present invention using lysozyme two-dimensional nano-film as anti-biotic material, anti-biotic material group is divided into natural biological point
Son --- lysozyme, lysozyme can obtain extensively from nature, not need artificial synthesized, avoid traditional anti-biotic material and synthesizing
When caused by environmental pollution, and lysozyme two-dimensional nano-film transparency is higher (appearance color for not influencing substrate), roughness
Low, thickness is controllable (30nm and its more than), does not need the cumbersome techniques such as spin coating, active medium load, easy to operate, need to only make
The lysozyme two-dimensional nano that growth in situ will be prepared in substrate surface or directly during standby lysozyme two-dimensional nano-film
Film is adhered to substrate surface, and with growth in situ or can be adhered to substrate surface of various shapes, large area easy to accomplish
Preparation.
2, hydrophiling may be implemented to the surface of substrate using lysozyme two-dimensional nano-film as anti-biotic material in the present invention
Processing, and lysozyme two-dimensional nano-film itself is positively charged, and water-wetted surface can improve bio-compatible in biomedical applications
Property, positive surface charge provides a potential mechanism to kill bacterium.
3, the present invention is using lysozyme two-dimensional nano-film as anti-biotic material, with wide spectrum and efficient anti-microbial property,
Antimicrobial spectrum can be expanded to gram-positive bacteria, Gram-negative bacteria and fungi three classes from classic gram-positive bacteria one kind bacterium
Bacterium, and good antimicrobial efficiency is presented to these three types of bacterium, antimicrobial efficiency was all up substantially at 2 hours to 90% or more.
4, the present invention is using lysozyme two-dimensional nano-film as anti-biotic material, to cytotoxic, kills effect to bacterium
Be significantly better than other antibacterial agents with healthy cell nontoxicity, thus be alternatively arranged as a kind of ideal antibacterial food packaging material and
Medical applications material.
5, the present invention has good adherency to substrate to be protected using lysozyme two-dimensional nano-film as anti-biotic material
Property, but to cell (blood platelet) without adsorptivity, therefore there is good soil resistance to cell.
Detailed description of the invention
Fig. 1 is the photo of medical plastic catheter surface in situ growth lysozyme two-dimensional nano-film in embodiment 2.
Fig. 2 is the photo of 3 surgery knife surface adhesion lysozyme two-dimensional nano-film of embodiment.
Fig. 3 is antimicrobial efficiency of the lysozyme two-dimensional nano-film to staphylococcus aureus of various concentration lysozyme preparation
Figure.
Fig. 4 is antimicrobial efficiency figure of the lysozyme two-dimensional nano-film to Escherichia coli of various concentration lysozyme preparation.
Fig. 5 is antimicrobial efficiency figure of the lysozyme two-dimensional nano-film to candida albicans of various concentration lysozyme preparation.
The change that Fig. 6 is lysozyme two-dimensional nano-film to the antimicrobial efficiency of staphylococcus aureus and Escherichia coli at any time
Change curve graph.
Fig. 7 is laser confocal microscope figure of the lysozyme two-dimensional nano-film to normal cell.
Fig. 8 be sheet glass (left side), growth in situ lysozyme two-dimensional nano-film sheet glass (right side) to normal cell, (blood is small
Plate) absorption scanning electron microscope.
Specific embodiment
The present invention is described in more detail with reference to the accompanying drawings and examples, but protection scope of the present invention is not limited only to
These embodiments.
Embodiment 1
Application of the lysozyme two-dimensional nano-film as anti-biotic material, specifically used method are as follows:
0.1433g tri- (2- carboxyethyl) phosphine is added to the 4- hydroxyethyl piperazineethanesulfonic acid that 10mL10mmol/LpH value is 7.4
In buffer solution, pH value is adjusted to 6.4 with NaOH, is configured to the 4- hydroxyethyl piperazine second of three (2- carboxyethyl) phosphines of 50mmol/L
Sulfonic acid buffer solution；It is molten that the 4- hydroxyethyl piperazineethanesulfonic acid buffering that 10mL10mmol/LpH value is 7.4 is added in 40mg lysozyme
In liquid, it is configured to the 4- hydroxyethyl piperazineethanesulfonic acid buffer solution of 4mg/mL lysozyme；Take (the 2- carboxylic second of 10mL50mmol/L tri-
Base) phosphine 4- hydroxyethyl piperazineethanesulfonic acid buffer solution and 10mL4mg/mL lysozyme 4- hydroxyethyl piperazineethanesulfonic acid buffering it is molten
Liquid is uniformly mixed, and is stored at room temperature 50 minutes, forms thin film, i.e. lysozyme two-dimensional nano-film on mixed liquor surface, then
Lysozyme two-dimensional nano-film is transferred to glass sheet surface using Ago-Gel as medium, plays the role of antimicrobial protection.
Embodiment 2
Application of the lysozyme two-dimensional nano-film as anti-biotic material, specifically used method are as follows:
It is slow that the trishydroxymethylaminomethane that 10mL10mmol/LpH value is 7.4 is added in (2- carboxyethyl) phosphine of 0.1433g tri-
It rushes in solution, adjusts pH value to 6.4 with NaOH, be configured to the trishydroxymethylaminomethane of three (2- carboxyethyl) phosphines of 50mmol/L
Buffer solution；40mg lysozyme is added in the trishydroxymethylaminomethane buffer solution that 10mL10mmol/LpH value is 7.4, is matched
The trishydroxymethylaminomethane buffer solution of the lysozyme of 4mg/mL is made；By three (2- carboxyethyl) phosphines of 10mL50mmol/L
Trishydroxymethylaminomethane buffer solution mixes with the trishydroxymethylaminomethane buffer solution of the lysozyme of 10mL 4mg/mL
It is even, medical plastic conduit is inserted into mixing liquid, is stored at room temperature 50 minutes, is directly formed in medical plastic conduit surfaces externally and internally
One layer of self-assembled film (see Fig. 1), i.e. lysozyme two-dimensional nano-film, play the role of antimicrobial protection.
Embodiment 3
Application of the lysozyme two-dimensional nano-film as anti-biotic material, specifically used method are as follows:
The preparation method of lysozyme two-dimensional nano-film is same as Example 1 in the present embodiment, then with Ago-Gel
Lysozyme two-dimensional nano-film is transferred to surgical knife surface (see Fig. 2) as medium, plays the role of antimicrobial protection.
Embodiment 4
Application of the lysozyme two-dimensional nano-film as anti-biotic material, specifically used method are as follows:
The preparation method of lysozyme two-dimensional nano-film is same as Example 1 in the present embodiment, then with Ago-Gel
Lysozyme two-dimensional nano-film is transferred to the surface of paper as medium, plays the role of antimicrobial protection.
Embodiment 5
Application of the lysozyme two-dimensional nano-film as anti-biotic material, specifically used method are as follows:
The preparation method of lysozyme two-dimensional nano-film is same as Example 1 in the present embodiment, then with Ago-Gel
Lysozyme two-dimensional nano-film is transferred to the surface of bending plastic pipe as medium, plays the role of antimicrobial protection.
Embodiment 6
Application of the lysozyme two-dimensional nano-film as anti-biotic material, specifically used method are as follows:
In order to prove beneficial effects of the present invention, inventor has carried out a large amount of laboratory research test, has specifically tested feelings
Condition is as follows:
1, Anti-microbial Performance Tests
0.1433g tri- (2- carboxyethyl) phosphine is added to the 4- hydroxyethyl piperazineethanesulfonic acid that 10mL10mmol/LpH value is 7.4
In buffer solution, pH value is adjusted to 6.4 with NaOH, is configured to the 4- hydroxyethyl piperazine second of three (2- carboxyethyl) phosphines of 50mmol/L
Sulfonic acid buffer solution；Lysozyme is added in the 4- hydroxyethyl piperazineethanesulfonic acid buffer solution that 10mL10mmol/LpH value is 7.4,
It is configured to the 4- hydroxyethyl piperazineethanesulfonic acid buffer solution of 2mg/mL, 4mg/mL, 8mg/mL lysozyme respectively；It takes
The 4- hydroxyethyl piperazineethanesulfonic acid buffer solution of 10mL50mmol/L tri- (2- carboxyethyl) phosphine and 10mL various concentration lysozyme
4- hydroxyethyl piperazineethanesulfonic acid buffer solution is uniformly mixed, and is stored at room temperature 50 minutes, forms thin film on mixed liquor surface, i.e.,
Lysozyme two-dimensional nano-film.Lysozyme two-dimensional nano-film is transferred to sheet glass table respectively using Ago-Gel as medium
Face.
By staphylococcus aureus strains, coli strain respectively in LB culture medium 37 DEG C cultivate 16 hours, then
Bacterial suspension is inoculated into 37 DEG C of cultures in fresh LB, is 7.4 by inoculum pH value when OD600=0.5
PBS buffer solution be diluted to 5 × 105CFU/mL, then the inoculum after 10.0 μ L dilution is seeded in adherency lysozyme two
On the sheet glass for tieing up nano thin-film, hatch 2 hours for 37 DEG C in 35mL sterile petri dish, then adding 2mLpH value is 7.4
PBS buffer solution rinses bacterium into 35mL sterile petri dish, and 10.0 μ L is taken to calculate clump count.As a result see Fig. 3 and Fig. 4.
Beads bacteria strain is cultivated 18 hours for 28 DEG C in the culture medium of the culture solution containing yeast malt, to OD600=1.0
When, beads bacteria culture fluid is diluted to 5 × 105CFU/mL with the PBS buffer solution that pH value is 7.4, then will be after 10.0 μ L dilution
Beads bacteria culture fluid is seeded on the sheet glass of adherency lysozyme two-dimensional nano-film, 28 DEG C of hatchings in 35mL sterile petri dish
It 2 hours, then adds the PBS buffer solution that 2mLpH value is 7.4 and rinses candida albicans into 35mL sterile petri dish, 10.0 μ L is taken to count
Calculate clump count.As a result see Fig. 5.
It, can be to gram by Fig. 3~5 as it can be seen that lysozyme two-dimensional nano-film has wide spectrum and efficient anti-microbial property
Three kinds of positive bacteria, Gram-negative bacteria and candida albicans bacterium generate resistance, and good antimicrobial efficiency, antibacterial are presented to these three types of bacterium
Efficiency can reach 90% or more at 2 hours substantially.And two wiener of lysozyme of the increase preparation with lysozyme concentration
The antibiotic property of rice film is also raising (can reach 95% or more).
Inventor tests the 4- hydroxyethyl piperazineethanesulfonic acid buffer solution preparation of 4mg/mL lysozyme according to the method described above
Lysozyme two-dimensional nano-film be adhered on sheet glass to the antimicrobial efficiency of staphylococcus aureus, Escherichia coli at any time
Variation, is as a result shown in Fig. 6.As seen from the figure, lysozyme two-dimensional nano-film murders efficiency to staphylococcus aureus, Escherichia coli
It can reach 90% or more at 2 hours.
2, cell toxicity test
Fresh human erythrocyte (hRBC) is centrifuged 15 minutes with the tris buffer that pH value is 7.4 at 1500 revs/min
Three times, obtaining the hRBC concentration to suspend in tris buffer is 5.0% (v/v) for washing, then will adhere to bacteriolyze in embodiment 1
The sheet glass of enzyme two-dimensional nano-film is immersed in the tris buffer of the above-mentioned hRBC of 2.0mL, under 100 revs/min of revolving speed
37 DEG C hatch 1 hour, are later centrifuged sheet glass 5 minutes at 1500 revs/min.Using confocal laser scanning microscope, knot
Fruit sees Fig. 7.As seen from the figure, human erythrocyte does not deform or ruptures, and illustrates lysozyme two-dimensional nano-film conduct
Anti-biotic material is to cytotoxic.
3, cell adsorptivity is tested
It is slow that the trishydroxymethylaminomethane that 10mL10mmol/LpH value is 7.4 is added in (2- carboxyethyl) phosphine of 0.1433g tri-
It rushes in solution, adjusts pH value to 4.5 with NaOH, be configured to the trishydroxymethylaminomethane of three (2- carboxyethyl) phosphines of 50mmol/L
Buffer solution；20mg lysozyme is added in the trishydroxymethylaminomethane buffer solution that 10mL10mmol/LpH value is 7.4, is matched
The trishydroxymethylaminomethane buffer solution of the lysozyme of 2mg/mL is made；By three (2- carboxyethyl) phosphines of 10mL50mmol/L
Trishydroxymethylaminomethane buffer solution mixes with the trishydroxymethylaminomethane buffer solution of the lysozyme of 10mL 2mg/mL
It is even, sheet glass is affixed on mixing liquid surface, is stored at room temperature 50 minutes, it is thin directly to form one layer of self assembly in glass sheet surface
Film, i.e. lysozyme two-dimensional nano-film, play the role of antimicrobial protection.
Healthy Rabbits new blood is centrifuged 15 minutes at 1000 revs/min, obtains thrombocyte plasma (PRP).By blank glass
Glass piece and the sheet glass of adherency lysozyme two-dimensional nano-film are immersed in hatching 1 in 37 DEG C of PBS buffer solution (pH value 7.4)
Hour, it is then injected into 1mLPRP, is hatched 30 minutes at 37 DEG C, blank glass piece is rinsed with fresh PBS buffer solution and adheres to molten
The sheet glass of bacterium enzyme two-dimensional nano-film 3~5 times.Finally, in 4 DEG C of processing sheet glass and being glued with 2.5wt% glutaraldehyde water solution
The sheet glass of attached lysozyme two-dimensional nano-film is cleaned after a whole night with fresh PBS buffer solution, then with 25%, 50%,
75%, the ethanol dehydration of 100% gradient concentration.It is observed using scanning electron microscope, as a result sees Fig. 8.As seen from the figure, bacteriolyze
Enzyme two-dimensional nano-film has good soil resistance without adsorptivity, therefore to cell to cell (blood platelet).
Claims (8)
1. a kind of anti-biotic material lysozyme two-dimensional nano-film, it is characterised in that the lysozyme two-dimensional nano-film is by with lower section
Method is prepared: directly contacting substrate to be protected with solution surface, the nano particle for generating lysozyme phase transition passes through
Surface and interface induction directly consolidates surface self-organization in liquid and forms two-dimensional nano-film, and growth in situ rises in substrate surface to be protected
The effect of antimicrobial protection；Or the lysozyme two-dimensional nano-film directly prepared is attached directly to substrate table to be protected
Face；
Wherein the solution is the buffer solution comprising lysozyme；
The lysozyme two-dimensional nano-film only includes that lysozyme is antibacterial components；
The substrate to be protected is the substrate for having resistance to water；
Preparation process does not need spin coating or active medium load.
2. anti-biotic material lysozyme two-dimensional nano-film according to claim 1, it is characterised in that: described to have to water
The substrate of resistance is silicon wafer, glass, silica, polyethylene terephthalate, fiber, hairline, polyimides, gathers
Ethylene, polypropylene, any one in polycarbonate.
3. anti-biotic material lysozyme two-dimensional nano-film according to claim 1, it is characterised in that: the lysozyme two dimension
Nano thin-film the preparation method comprises the following steps:
It is slow that 0.1433g tri- (2- carboxyethyl) phosphine is added to the 4- hydroxyethyl piperazineethanesulfonic acid that 10mL 10mmol/L pH value is 7.4
It rushes in solution, adjusts pH value to 6.4 with NaOH, be configured to the 4- hydroxyethyl piperazine second sulphur of three (2- carboxyethyl) phosphines of 50mmol/L
Acid buffering solution；It is molten that the 4- hydroxyethyl piperazineethanesulfonic acid buffering that 10mL 10mmol/L pH value is 7.4 is added in 40mg lysozyme
In liquid, it is configured to the 4- hydroxyethyl piperazineethanesulfonic acid buffer solution of 4mg/mL lysozyme；Take (the 2- carboxylic second of 10mL 50mmol/L tri-
Base) phosphine 4- hydroxyethyl piperazineethanesulfonic acid buffer solution and 10mL 4mg/mL lysozyme 4- hydroxyethyl piperazineethanesulfonic acid buffer
Solution is uniformly mixed, and is stored at room temperature 50 minutes, i.e., lysozyme two-dimensional nano-film is formed on mixed liquor surface, then with agarose
Lysozyme two-dimensional nano-film is transferred to glass sheet surface as medium by gel.
4. anti-biotic material lysozyme two-dimensional nano-film according to claim 1, it is characterised in that: the lysozyme two dimension
Nano thin-film the preparation method comprises the following steps: by (2- carboxyethyl) phosphine of 0.1433g tri- be added 10mL 10mmol/L pH value be 7.4 three hydroxyls
In aminomethane buffer solution, pH value is adjusted to 6.4 with NaOH, is configured to the three of three (2- carboxyethyl) phosphines of 50mmol/L
Hydroxymethyl aminomethane buffer solution；The trihydroxy methyl amino that 10mL 10mmol/L pH value is 7.4 is added in 40mg lysozyme
In methane buffer solution, it is configured to the trishydroxymethylaminomethane buffer solution of the lysozyme of 4mg/mL；By 10mL 50mmol/L
Three (2- carboxyethyl) phosphines trishydroxymethylaminomethane buffer solution and 10mL 4mg/mL lysozyme trihydroxy methyl amino
Methane buffer solution is uniformly mixed, and medical plastic conduit is inserted into mixing liquid, is stored at room temperature 50 minutes, leads in medical plastic
Pipe surfaces externally and internally directly forms one layer of self-assembled film, i.e. lysozyme two-dimensional nano-film.
5. application method of the lysozyme two-dimensional nano-film described in claim 1 as anti-biotic material, it is characterised in that: making
Directly substrate to be protected is contacted with solution surface during standby lysozyme two-dimensional nano-film, keeps lysozyme phase transition raw
At nano particle by surface and interface induce directly liquid consolidate surface self-organization formation two-dimensional nano-film, growth in situ is in wait protect
The substrate surface of shield, plays antimicrobial protection；
Wherein the solution is the buffer solution comprising lysozyme；
The lysozyme two-dimensional nano-film only includes that lysozyme is antibacterial components；
The substrate to be protected is the substrate for having resistance to water；
Preparation process does not need spin coating or active medium load；
And in use only need to during preparing lysozyme two-dimensional nano-film growth in situ and substrate surface or
The lysozyme two-dimensional nano-film prepared is directly adhered to substrate surface, and with growth in situ or various shapes can be adhered to
The substrate surface of shape, the preparation of large area easy to accomplish.
6. application method of the anti-biotic material lysozyme two-dimensional nano-film according to claim 5 as anti-biotic material,
It is characterized in that: the lysozyme two-dimensional nano-film prepared is attached directly to substrate surface to be protected, play antimicrobial protection
Effect, wherein the substrate to be protected is the substrate for having sensibility to water.
7. application method of the anti-biotic material lysozyme two-dimensional nano-film according to claim 6 as anti-biotic material,
Be characterized in that: it is described to water have sensibility substrate be paper, timber, cloth, drawing, copper mesh, iron, aluminium, nickel, platinum, gold,
Copper, silver, any one in electronic device.
8. application of the lysozyme two-dimensional nano-film described in claim 1 as anti-biotic material, the bacterium is gram sun
Property bacterium, Gram-negative bacteria and fungi, wherein gram-positive bacteria be staphylococcus aureus, Diplococcus pneumopniae or tetanus bar
Bacterium, Gram-negative bacteria are Escherichia coli, shigella dysenteriae or typhoid bacillus, and fungi is candida albicans or fugue bacterium.
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| CN108310447A (en) * | 2018-03-22 | 2018-07-24 | 陕西师范大学 | A kind of bacteriolyze enzyme modification hospital gauze |
| CN108854599B (en) * | 2018-05-14 | 2020-10-16 | 陕西师范大学 | Dialysis membrane based on cross-linked lysozyme and application thereof |
| CN115365494B (en) * | 2022-09-13 | 2023-07-07 | 嘉兴学院 | Preparation method of silver-coated copper powder and application of silver-coated copper powder in conductive paste |
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| CN105475359A (en) | 2016-04-13 |
| CN109845761B (en) | 2021-04-06 |
| CN105475359B (en) | 2020-06-26 |
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