CN201313888Y - Microbe inoculation-cultivation device - Google Patents
Microbe inoculation-cultivation device Download PDFInfo
- Publication number
- CN201313888Y CN201313888Y CN 200820169980 CN200820169980U CN201313888Y CN 201313888 Y CN201313888 Y CN 201313888Y CN 200820169980 CN200820169980 CN 200820169980 CN 200820169980 U CN200820169980 U CN 200820169980U CN 201313888 Y CN201313888 Y CN 201313888Y
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- CN
- China
- Prior art keywords
- base
- sealing
- inoculation
- sealing cover
- cavities
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model relates to a microbe inoculation-cultivation device. A traditional culture vessel is not suitable for the utilizing requirements of the modern digital image technology. The microbe inoculation-cultivation device comprises a base and a sealing cover which is connected with the base in a matching manner; wherein the base adopts the structure of a hollow flat rectangular solid, a plurality of inoculation cavities which are arrayed according to a matrix are arranged at the bottom part of the base, and adjacent inoculation cavities are separated by separation fences. The sealing cover also adopts the structure of a hollow flat rectangular solid, a plurality of sealing strip groups are vertically arranged in the sealing cover, each sealing strip group comprises two sealing strips which are correspondingly arranged, a sealing groove is formed between the two sealing strips, and the sealing strip groups are circled into separation cavities. The quantity of the sealing grooves and the separation fences is the same, and the positions are corresponding. The quantity of the separation cavities and the inoculation cavities is the same, and the positions are corresponding. On one device, a plurality of different agar nutrient media is allowed to be loaded; the microbe inoculation-cultivation device is suitable for the growth of germs in wider range; the detection range is widened, and the detection time is saved.
Description
Technical field
The utility model belongs to the detection technique field, relates to a kind of microbial inoculant and culture apparatus.
Background technology
Microbe colony detects, and is the important work of fundamental sum in fields such as health care, inspection and quarantine, food safety, disease control.Adopt a kind of glass culture dish traditionally, the agar that will contain nutritive ingredient is earlier poured in it, and the measured matter that will contain bacterium with inoculating needle is seeded in agar surface again, cultivation by certain temperature, bacterium grows up and forms bacterium colony, again by naked eyes or image observation system, detects colony number wherein.
Different agar nutritive mediums is fit to different bacterial growths, so traditional method is unfavorable for the growth of various bacteria, unless adopt a plurality of culture dish to realize this requirement.In addition, traditional method can only detect colony number, and what bacterium can not determine the end is.The modern digital image technique is convenient greatly and accelerated observation to culture dish, and can realize certain analysis and judgement function.And traditional culture dish has been not suitable for the service requirements of modern digital image technique very much.
Have multiple nutrients agar simultaneously if make in the vessel, be seeded in detected material on this multiple nutrients base simultaneously after, just help the growth of various different bacterium.By the cultivation under the certain temperature, and adopt Digital image technology to observe and detect, not only can detect various microorganisms contained in the measured matter faster, and, also help preliminary judgement bacterial species by analysis to the growthhabit of bacterium on the Different Nutrition liquid.The development and the demand that adapt to modern medical service health and food safety field microbial rapid detection.
Summary of the invention
The purpose of this utility model is exactly at the deficiencies in the prior art, thereby a kind of novel microorganism colony inoculation and culture apparatus that many nutrition base adapts to many growth that have is provided.
The utility model comprises base and the sealing cover that is connected.Wherein base is the flat rectangular structure of hollow, its sidewall and closed bottom, open-top.The bottom of base is provided with a plurality of equally distributed depressions, forms (x+1) * (y+1) individual inoculation chamber, and a plurality of inoculations chamber is according to arranged.Separate by the isolated gate that intersects in length and breadth that vertically is provided with in the base between the adjacent inoculation chamber, wherein the quantity of lateral isolation grid is the x bar, and vertically the quantity of isolated gate is the y bar, and the top of isolated gate is concordant with the top of base.Sealing cover also is the flat rectangular structure of hollow, and its sidewall and top seal, bottom are opened.A plurality of sealed strip groups vertically are set in the sealing cover, and each sealed strip group comprises corresponding two sealed strips that are provided with, and forms sealing groove between two sealed strips, and the sealed strip group surrounds square space and constitutes separate cavities.Sealing groove in the sealing cover is identical with isolated gate quantity in the base, the position is corresponding, and the interior inoculation chamber quantity of the separate cavities in the sealing cover and base is identical, the position is corresponding.
The utility model allows to load multiple different agar nutrition base on a device, be fit to the more bacterial growth of wide region, widens sensing range, saves detection time.Adopt this device the analysis of the intravital microorganism growth form of different cavity to be compared, also help the preliminary judgement of realization microbe species by adopting the modern digital image technique.Isolated gate and sealing groove are to having good buffer action between the different cavitys, guaranteeing does not have cross infection between each cavity.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
Embodiment
A kind of microbial inoculant and culture apparatus comprise base 6 and sealing cover 1, and sealing cover 1 covers on the base 6.Base 6 is the flat rectangular structure of hollow, its sidewall and closed bottom, open-top.The bottom of base 6 is provided with four equally distributed depressions, forms four inoculation chambeies 3.Arrange according to each mode of two in length and breadth in four inoculation chambeies 3.Separate by each isolated gate 7 of one in length and breadth between adjacent two inoculation chambeies, isolated gate 7 is vertical with the bottom surface of base 6, and the top of isolated gate 7 is concordant with the top of base 6.
Sealing cover 1 also is the flat rectangular structure of hollow, and its sidewall and top seal, bottom are opened.Two sealed strip groups vertically are set in the sealing cover 1, and each sealed strip group comprises formation sealing groove 4 between corresponding 5, two sealed strips 5 of two sealed strips that are provided with, and the sealed strip group surrounds square space and constitutes separate cavities 2.Sealing groove 4 in the sealing cover 1 is corresponding with isolated gate 7 positions in the base 6, and the separate cavities 2 in the sealing cover 1 is corresponding with 3 positions, inoculation chamber in the base 6.
When reality is used, various agar nutritive medium can be injected each different vaccination chamber on the base.Then measured matter is seeded in the agar surface in each chamber, again sealing cover is covered on the base, can cultivate at a certain temperature.The isolation fully between each inoculation chamber is guaranteed in the cooperation of sealing groove and isolated gate.
Claims (1)
1, a kind of microbial inoculant and culture apparatus comprise the base and the sealing cover that are connected, it is characterized in that: described base is the flat rectangular structure of hollow, its sidewall and closed bottom, open-top; The bottom of base is provided with a plurality of equally distributed depressions, forms (x+1) * (y+1) individual inoculation chamber, and a plurality of inoculations chamber is according to arranged; Separate by the isolated gate that intersects in length and breadth that vertically is provided with in the base between the adjacent inoculation chamber, wherein the quantity of lateral isolation grid is the x bar, and vertically the quantity of isolated gate is the y bar, and the top of isolated gate is concordant with the top of base; Described sealing cover is the flat rectangular structure of hollow, and its sidewall and top seal, bottom are opened; A plurality of sealed strip groups vertically are set in the sealing cover, and each sealed strip group comprises corresponding two sealed strips that are provided with, and forms sealing groove between two sealed strips, and the sealed strip group surrounds square space and constitutes separate cavities; Sealing groove in the sealing cover is identical with isolated gate quantity in the base, the position is corresponding, and the interior inoculation chamber quantity of the separate cavities in the sealing cover and base is identical, the position is corresponding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820169980 CN201313888Y (en) | 2008-12-15 | 2008-12-15 | Microbe inoculation-cultivation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820169980 CN201313888Y (en) | 2008-12-15 | 2008-12-15 | Microbe inoculation-cultivation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201313888Y true CN201313888Y (en) | 2009-09-23 |
Family
ID=41125680
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200820169980 Expired - Lifetime CN201313888Y (en) | 2008-12-15 | 2008-12-15 | Microbe inoculation-cultivation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201313888Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101423794B (en) * | 2008-12-15 | 2011-09-21 | 杭州电子科技大学 | Multi-nutrient microbial inoculation and culture device |
| CN102753675A (en) * | 2010-01-14 | 2012-10-24 | Emd密理博公司 | Membrane transfer method and tool |
| CN102816683A (en) * | 2012-08-01 | 2012-12-12 | 江苏嘉语生物医药技术有限公司 | Biochemical culture and detection device as well as detection method thereof |
| CN105209911A (en) * | 2013-03-11 | 2015-12-30 | 贝克顿·迪金森公司 | Bacterial detection cartridge |
-
2008
- 2008-12-15 CN CN 200820169980 patent/CN201313888Y/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101423794B (en) * | 2008-12-15 | 2011-09-21 | 杭州电子科技大学 | Multi-nutrient microbial inoculation and culture device |
| CN102753675A (en) * | 2010-01-14 | 2012-10-24 | Emd密理博公司 | Membrane transfer method and tool |
| CN102753675B (en) * | 2010-01-14 | 2014-07-16 | Emd密理博公司 | Membrane transfer method and tool |
| US9732371B2 (en) | 2010-01-14 | 2017-08-15 | Emd Millipore Corporation | Membrane transfer method and tool |
| CN102816683A (en) * | 2012-08-01 | 2012-12-12 | 江苏嘉语生物医药技术有限公司 | Biochemical culture and detection device as well as detection method thereof |
| CN105209911A (en) * | 2013-03-11 | 2015-12-30 | 贝克顿·迪金森公司 | Bacterial detection cartridge |
| US9909162B2 (en) | 2013-03-11 | 2018-03-06 | Becton, Dickinson And Company | Bacterial detection cartridge |
| US10557163B2 (en) | 2013-03-11 | 2020-02-11 | Becton Dickinson And Company | Bacterial detection cartridge |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20090923 Effective date of abandoning: 20081215 |