CN201704330U - Inoculation culture apparatus capable of simultaneously and stably contacting with surface of culture medium with multi-microorganisms - Google Patents

Inoculation culture apparatus capable of simultaneously and stably contacting with surface of culture medium with multi-microorganisms Download PDF

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Publication number
CN201704330U
CN201704330U CN 201020226241 CN201020226241U CN201704330U CN 201704330 U CN201704330 U CN 201704330U CN 201020226241 CN201020226241 CN 201020226241 CN 201020226241 U CN201020226241 U CN 201020226241U CN 201704330 U CN201704330 U CN 201704330U
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culture
inoculating needle
support bar
inoculating
culture dish
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CN 201020226241
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于大胜
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions

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  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses an inoculation culture apparatus capable of simultaneously and stably contacting with a surface of a culture medium with multi-microorganisms which consists of inoculating needles (1) and a glass culture dish (2); the inoculation culture apparatus capable of simultaneously and stably contacting with the surface of the culture medium with multi-microorganisms is characterized in that culture cavities (4) holding different culture mediums (11) and spaced by spacing walls (3) in equal distances are arranged at the surrounding inside the glass culture dish (2); floaters (6) supported by water inside a cylindrical spacing wall (5), upper vertical supporting bars (7) fixed on the floaters (6) and inoculating needle supporting bars (8) at the upper ends of the floaters are arranged in the center of the glass culture dish (2); and the inoculating needles (1) are inserted in the hole of the outer ends of the inoculating needle supporting bars (8) to lead the lower ends of the inoculating needle supporting bars (8) to be contacted with the surfaces of the culture mediums (11). The inoculation culture apparatus can simultaneously culture a plurality of germs, which can be suitable for the using requirements of modern digital image technologies. As the floaters are adopted to lead the heads of a plurality of inoculating needles to be stably contacted with the surfaces of the culture mediums of each own, the working efficiency is high.

Description

Multiple microorganism surely touches media surface inoculation culture device simultaneously
Technical field
The utility model relates to a kind of improvement of microbial inoculant incubator, microorganism belonging to genus test apparatus technical field, and specifically one kind of multiple microorganisms surely touch media surface inoculation culture device simultaneously.
Background technology
The microbe colony inoculation culture detects, and is an important job of aspects such as health care, inspection and quarantine, disease control, food safety.The used instrument of microbial inoculant cultivation at present is inoculating needle and a glass culture dish, in this glass culture dish, fill the agar nutrient solution, operator's hand takes the inoculating needle dip in microorganism with annotate the gently surface of the agar nutrient solution in glass culture dish of a small amount of micro-biological samples, sensation according to hand, make the end of inoculating needle just touch the surface of agar nutrient solution, can not puncture the surface of agar nutrient solution, cultivate at a certain temperature then, after bacterial growth is big, observe with the digital picture proofing unit again, detect colony number wherein.At present microbial inoculant is cultivated used instrument and the existing deficiency of method is: owing to adopt surface manual, just touch the agar nutrient solution with the end of feeling to make inoculating needle, so the surface of agar nutrient solution can not constant be just touched in the inoculating needle end, the time and puncture the surface of agar nutrient solution, the time and the phenomenon of leaving agar nutrient solution surface is difficult to avoid instability; Because the surface of agar nutrient solution changes sometimes, so when changing, manual operation often can not be followed the variation on agar nutrient solution surface rapidly and be changed; Owing to have only a substratum to put into the chamber in the culture dish, can only put into a kind of substratum, and different bacteriums needs different substratum, so this culture dish can only a kind of bacterium of inoculation culture, the service requirements of incompatible modernized Digital image technology.
Summary of the invention
The purpose of this utility model is to provide a kind of inoculating needle end constant that can not only make just to touch agar nutrient solution surface, can in time change automatically with the variation on agar nutrient solution surface, neither break away from agar nutrient solution surface, do not puncture agar nutrient solution surface again, and various bacteria can be concentrated in together the multiple microorganism of cultivating simultaneously and surely touch simultaneously media surface inoculation culture device.
For reaching above purpose, the technical scheme that the utility model adopted is: this multiple microorganism surely touches media surface inoculation culture device simultaneously, constitute by inoculating needle and glass culture dish, glass culture dish is circle basin shape, it is characterized in that the culture chamber that is divided into equidistant arrangement on every side by spaced walls in glass culture dish, in different culture chambers, fill different substratum, center in glass culture dish is provided with cylindric next door, in cylindric next door, fill water, be provided with float on the water surface in cylindric next door, fixing vertical support bar on the roof of float, inoculating needle support bar in the equidistant welding of the upper end of vertical support bar radial, in the hole of the external end head of inoculating needle support bar, insert inoculating needle, and with its position of bolting, the surface of substratum is touched in the lower end of inoculating needle.
The utility model is also implemented by following measure: the culture chamber in the described glass culture dish is established 6-10, and corresponding therewith, the number that inoculating needle is established is identical with the number of culture chamber, is fixed on the upper end of vertical support bar respectively by corresponding inoculating needle support bar.
The beneficial effects of the utility model are: compare with culture dish with the inoculating needle of present use, owing in a culture dish, established a plurality of culture chambers, put into different substratum in each culture chamber, cultivate different bacteriums, so, can once cultivate various bacteria simultaneously, adapt to the service requirements of modernized Digital image technology.Because each inoculating needle all adopts inoculating needle support bar separately to fix, it makes it stable by same float, so the head of a plurality of inoculating needles is stable simultaneously touches separately media surface, can raise labour efficiency.
Description of drawings
Fig. 1, be structure schematic top plan view of the present utility model.
Fig. 2, be that structure of the present utility model is along A-A section cross-sectional schematic.
Fig. 3, axially analyse and observe enlarged diagram for inoculating needle of the present utility model.
Embodiment
Make the utility model with reference to Fig. 1,2,3.This multiple microorganism surely touches media surface inoculation culture device simultaneously, constitute by inoculating needle 1 and glass culture dish 2, glass culture dish 2 is circle basin shape, it is characterized in that the culture chamber 4 that is divided into equidistant arrangement on every side by spaced walls 3 in glass culture dish 2, in different culture chamber 4, fill different substratum 11, center in glass culture dish 2 is provided with cylindric next door 5, in cylindric next door 5, fill water 10, be provided with float 6 on the water surface in cylindric next door 5, fixing vertical support bar 7 on the roof of float 6, inoculating needle support bar 8 in the equidistant welding of the upper end of vertical support bar 7 radial, in the hole of the external end head of inoculating needle support bar 8, insert inoculating needle 1, and fix its position with bolt 9, the surface of substratum 11 is touched in the lower end of inoculating needle 1, after adjusting, make the end of inoculating needle 1 touch the surface of substratum 11 all the time by float 6, so neither can leave the surface of substratum 11, also can not thrust in the surface of substratum 11, stable be in a position.
The utility model is also implemented by following measure: the culture chamber 4 in the described glass culture dish 2 is established 6-10, corresponding therewith, the number that inoculating needle 1 is established is identical with the number of culture chamber 4, is fixed on the upper end of vertical support bar 7 respectively by corresponding inoculating needle support bar 8.
When using the utility model, different inoculating needles 1 insert different bacteriums, put into the needed substratum 11 of this kind bacterium in corresponding culture chamber 4, can once cultivate 6-10 kind bacterium, by adjusting, make the end of each inoculating needle 1 all just touch corresponding substratum 11 surfaces, and fixing; Described inoculating needle 1 is the Taper Pipe shape, during inoculation, the sampling bacterium is splashed into from the suitable for reading of Taper Pipe, and the sampling bacterium can flow to substratum 11 surfaces along the inwall of Taper Pipe, carries out inoculation culture.

Claims (2)

1. one kind of multiple microorganisms surely touch media surface inoculation culture device simultaneously, constitute by inoculating needle (1) and glass culture dish (2), glass culture dish (2) is circle basin shape, it is characterized in that the culture chamber (4) that is divided into equidistant arrangement on every side by spaced walls (3) in glass culture dish (2), in different culture chamber (4), fill different substratum (11), center in glass culture dish (2) is provided with cylindric next door (5), in cylindric next door (5), fill water (10), be provided with float (6) on the water surface in cylindric next door (5), the fixing vertical support bar (7) that goes up on the roof of float (6), inoculating needle support bar (8) in the equidistant welding of the upper end radial of vertical support bar (7), in the hole of the external end head of inoculating needle support bar (8), insert inoculating needle (1), and fix its position with bolt (9), the surface of substratum (11) is touched in the lower end of inoculating needle (1).
2. multiple microorganism according to claim 1 surely touches media surface inoculation culture device simultaneously, it is individual to it is characterized in that the interior culture chamber (4) of described glass culture dish (2) is established 6-10, corresponding therewith, the number that inoculating needle (1) is established is identical with the number of culture chamber (4), is fixed on the upper end of vertical support bar (7) respectively by corresponding inoculating needle support bar (8).
CN 201020226241 2010-06-17 2010-06-17 Inoculation culture apparatus capable of simultaneously and stably contacting with surface of culture medium with multi-microorganisms Expired - Fee Related CN201704330U (en)

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CN 201020226241 CN201704330U (en) 2010-06-17 2010-06-17 Inoculation culture apparatus capable of simultaneously and stably contacting with surface of culture medium with multi-microorganisms

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CN 201020226241 CN201704330U (en) 2010-06-17 2010-06-17 Inoculation culture apparatus capable of simultaneously and stably contacting with surface of culture medium with multi-microorganisms

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104498349A (en) * 2014-12-04 2015-04-08 济南朗迈生物科技有限公司 Method and device for culture medium inoculation by using gravity to control strength
CN107177484A (en) * 2017-06-09 2017-09-19 天津施特雷生物科技股份有限公司 A kind of disposable sterilized microbial culture dish

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104498349A (en) * 2014-12-04 2015-04-08 济南朗迈生物科技有限公司 Method and device for culture medium inoculation by using gravity to control strength
CN104498349B (en) * 2014-12-04 2016-05-18 济南朗迈生物科技有限公司 A kind of culture medium inoculated method and apparatus that utilizes GRAVITY CONTROL dynamics
CN107177484A (en) * 2017-06-09 2017-09-19 天津施特雷生物科技股份有限公司 A kind of disposable sterilized microbial culture dish

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Granted publication date: 20110112

Termination date: 20110617