CN201704329U - Microorganism incubator on automatic constant touch agar surface - Google Patents

Microorganism incubator on automatic constant touch agar surface Download PDF

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Publication number
CN201704329U
CN201704329U CN 201020226238 CN201020226238U CN201704329U CN 201704329 U CN201704329 U CN 201704329U CN 201020226238 CN201020226238 CN 201020226238 CN 201020226238 U CN201020226238 U CN 201020226238U CN 201704329 U CN201704329 U CN 201704329U
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culture dish
glass culture
agar
sub
nutrient solution
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Expired - Fee Related
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CN 201020226238
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Chinese (zh)
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于大胜
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses a microorganism incubator on an automatic constant touch agar surface, which comprises an incubation needle (1) and a glass culture dish (2), and is characterized in that the outer side of the glass culture dish (2) is provided with a circular sink (4); the bottom part in the glass culture dish (2) is provided with a communicating pipe (5) which is communicated with the circular sink (4); a pressure balance piston (6) is arranged in the communicating pipe (5); four floaters (7) are symmetrically arranged in the circular sink (4); the four floaters (7) are respectively connected with four support rods of a crisscross sub-frame (8); the center of the crisscross sub-frame (8) is provided with a through hole; and the incubation needle (1) is inserted into the through hole in the center of the crisscross sub-frame (8). In the incubator, because a pressure balance principle is adopted, through the floaters and the supports connected therewith, the end of the incubation needle can constantly and rightly touch the surface of agar culture fluid, is not separated from and does not pierce the surface of the agar culture fluid, so that the use is convenient and labor saving.

Description

Automatic constant touches the microbial inoculant device of agar surface
Technical field
The utility model relates to a kind of improvement of microbial inoculant device, microorganism belonging to genus test apparatus technical field, and specifically a kind of automatic constant touches the microbial inoculant device of agar surface.
Background technology
Microbial inoculant is cultivated, and is an important job of aspects such as health care, inspection and quarantine, disease control, food safety, is the prerequisite of microorganism detection, check, is the basis to bacterium observation.The used instrument of microbial inoculant cultivation at present is inoculating needle and glass culture dish, in glass culture dish, fill the agar nutrient solution, operator's hand takes the inoculating needle dip in microorganism with annotate the gently surface of the agar nutrient solution in glass culture dish of a small amount of micro-biological samples, sensation according to hand, make the end of inoculating needle just touch the surface of agar nutrient solution, can not leave or puncture the surface of agar nutrient solution, cultivate at a certain temperature then, after bacterial growth is big, observe at microscopically again.Thisly adopt deficiency manual, that just touch the surface of agar nutrient solution with the end of feeling to make inoculating needle to be: because hand is on slippery ground fixed, so the surface of agar nutrient solution can not constant be just touched in the inoculating needle end, the time and puncture the surface of agar nutrient solution, the time and the phenomenon of leaving agar nutrient solution surface be difficult to avoid; And because the surface of agar nutrient solution changes sometimes, when changing, manual operation often can not be followed the variation on agar nutrient solution surface rapidly and be changed.
Summary of the invention
The purpose of this utility model be to provide a kind of can make inoculating needle end constant just touch agar nutrient solution surface and can in time change, neither break away from agar nutrient solution surface with the variation on agar nutrient solution surface automatically, microbial inoculant device that the automatic constant that do not puncture agar nutrient solution surface again touches agar surface.
For reaching above purpose, the technical scheme that the utility model adopted is: this automatic constant touches the microbial inoculant device of agar surface, constitute by inoculating needle and glass culture dish, glass culture dish is that open circle is box-like, in glass culture dish, fill the agar nutrient solution, it is characterized in that being provided with annular water tank in the outside of glass culture dish, in annular water tank, fill water, bottom in glass culture dish is provided with the communicating pipe that is connected with the annulus tank, in communicating pipe, be provided with the pressure equilibrium piston, the pressure of the agar nutrient solution in the hydraulic pressure that makes in the annulus tank by automatically moving of pressure equilibrium piston and the glass culture dish equates, symmetricly in the annulus tank be provided with four floats, on four floats, be connected with four poles of "+" sub-frame respectively, through hole is established at center at "+" sub-frame, insert inoculating needle in the central through hole of "+" sub-frame, the surface of agar nutrient solution is touched in the end of inoculating needle.
The utility model is also implemented by following measure: described inoculating needle is a tapered tube, lower port diameter is 1mm, and back cut diameter is 3-5mm, and microorganism sampling is splashed in inoculating needle suitable for reading, the microorganism sampling is freely glided along the tapered tube inwall, touches the surface of agar nutrient solution from the tapered tube end opening.
The beneficial effects of the utility model are: compare with glass culture dish by the used inoculating needle of the manual microbial inoculant cultivation of carrying out with present, owing to adopt pressure balance principle, support by float and connection thereof makes inoculating needle touch the surface of the agar nutrient solution in the glass culture dish, so can make inoculating needle end constant just touch agar nutrient solution surface, can in time change automatically with the variation on agar nutrient solution surface again, neither break away from agar nutrient solution surface, do not puncture agar nutrient solution surface again, easy to use, laborsaving.
Description of drawings,
Fig. 1 is a structure schematic top plan view of the present utility model.
Fig. 2 is that the utility model is along the A-A cross-sectional schematic.
Embodiment
Make the utility model with reference to Fig. 1,2.This automatic constant touches the microbial inoculant device of agar surface, constitute by inoculating needle 1 and glass culture dish 2, glass culture dish 2 is that open circle is box-like, in glass culture dish 2, fill agar nutrient solution 3, it is characterized in that being provided with annular water tank 4 in the outside of glass culture dish 2, in annular water tank 4, fill water, bottom in glass culture dish 2 is provided with the communicating pipe 5 that is connected with annulus tank 4, in communicating pipe 5, be provided with pressure equilibrium piston 6, the pressure of the agar nutrient solution 3 that the hydraulic pressure that makes in the annulus tank 4 by automatically moving of pressure equilibrium piston 6 and glass culture dish 2 are interior equates, thereby make the surface variation of the agar nutrient solution 3 in horizontal plane and the glass culture dish 2 in the annular water tank 4 consistent, symmetricly in annulus tank 4 be provided with four floats 7, on four floats 7, be connected with 8 four poles of "+" sub-frame respectively, through hole is established at center at "+" sub-frame 8, in the central through hole of "+" sub-frame 8, insert inoculating needle 1, control by inoculating needle 1 depth of penetration, make the end of inoculating needle 1 touch the surface of agar nutrient solution 3, because inoculating needle 1 is supported by four floats 7, so be stable, constant touches the surface of agar nutrient solution 3, if the surface of agar nutrient solution 3 changes, pressure equilibrium piston 6 in communicating pipe 5 just moves automatically, the water surface in the annulus tank 4 also changes thereupon, thereby four floats 7 are changed, inoculating needle 1 also changes, thereby the surface of agar nutrient solution 3 is neither left in the end that makes inoculating needle 1, also can not puncture the surface of agar nutrient solution 3.
The utility model is also implemented by following measure: described inoculating needle 1 is a tapered tube, lower port diameter is 1mm, back cut diameter is 3-5mm, microorganism sampling is splashed in inoculating needle 1 suitable for reading, the microorganism sampling is freely glided along the tapered tube inwall, touches the surface of agar nutrient solution 3 from the tapered tube end opening.

Claims (2)

1. an automatic constant touches the microbial inoculant device of agar surface, constitute by inoculating needle (1) and glass culture dish (2), glass culture dish (2) is that open circle is box-like, in glass culture dish (2), fill agar nutrient solution (3), it is characterized in that being provided with annular water tank (4) in the outside of glass culture dish (2), in annular water tank (4), fill water, bottom in glass culture dish (2) is provided with the communicating pipe (5) that is connected with annulus tank (4), in communicating pipe (5), be provided with pressure equilibrium piston (6), symmetric four floats (7) that are provided with in annulus tank (4), on four floats (7), be connected with (8) four poles of "+" sub-frame respectively, through hole is established at center at "+" sub-frame (8), inserts inoculating needle (1) in the central through hole of "+" sub-frame (8).
2. automatic constant according to claim 1 touches the microbial inoculant device of agar surface, it is characterized in that described inoculating needle (1) is a tapered tube, and lower port diameter is 1mm, and back cut diameter is 3-5mm.
CN 201020226238 2010-06-17 2010-06-17 Microorganism incubator on automatic constant touch agar surface Expired - Fee Related CN201704329U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201020226238 CN201704329U (en) 2010-06-17 2010-06-17 Microorganism incubator on automatic constant touch agar surface

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201020226238 CN201704329U (en) 2010-06-17 2010-06-17 Microorganism incubator on automatic constant touch agar surface

Publications (1)

Publication Number Publication Date
CN201704329U true CN201704329U (en) 2011-01-12

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105154322A (en) * 2015-09-09 2015-12-16 济南百博生物技术股份有限公司 Microbe inoculation device capable of automatically identifying culture dish and identifying method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105154322A (en) * 2015-09-09 2015-12-16 济南百博生物技术股份有限公司 Microbe inoculation device capable of automatically identifying culture dish and identifying method
CN105154322B (en) * 2015-09-09 2017-04-19 济南百博生物技术股份有限公司 Microbe inoculation device capable of automatically identifying culture dish and identifying method

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Granted publication date: 20110112

Termination date: 20110617