CN108680395A - It is a kind of to be easily used for the closed growth of microorganism continuous and gas-liquid sample sampler - Google Patents
It is a kind of to be easily used for the closed growth of microorganism continuous and gas-liquid sample sampler Download PDFInfo
- Publication number
- CN108680395A CN108680395A CN201810466319.1A CN201810466319A CN108680395A CN 108680395 A CN108680395 A CN 108680395A CN 201810466319 A CN201810466319 A CN 201810466319A CN 108680395 A CN108680395 A CN 108680395A
- Authority
- CN
- China
- Prior art keywords
- gas
- microorganism
- conical flask
- liquid
- silica gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/24—Suction devices
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Hydrology & Water Resources (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
It is a kind of to be easily used for the closed growth of microorganism continuous and gas-liquid sample sampler, belong to technical field of sewage in environmental protection, it is a kind of reactor assembly for being used for the closed culture of microorganism or microbial degradation sewage and being sampled for gas-liquid, the device provides closed environment for the polluter in the continuous culture of microorganism and pollution degradation water body or culture medium, possibility is provided to measure gas componant that microorganism generates or consumption, the concentration of the polluter volatilized from sewage can be measured simultaneously, solve the problems, such as caused by volatilizing due to polluter that concentration declines in water body and measurement result is inaccurate, simultaneously, the invention can easily sample gas-liquid sample, it is conveniently operated and measures.The structure of the present invention is simple, at low cost, facilitates observation.
Description
Technical field
The invention belongs to microculture in environmental protection and technical field of sewage, disclose a kind of easy microorganism
Reaction unit more particularly to a kind of reactor for being used for the closed culture of microorganism or microbial degradation sewage and being sampled for gas-liquid
Device.
Background technology
With the rapid growth of China's economic, industrial process is constantly pushed ahead, at the same time, generated industrial wastewater
Also increase year by year, if not administered, will seriously pollute environment, destroy the ecological balance, and influence mankind itself health and
Existence.Also, the feature that trade effluent gradually shows includes：High cod values, multiple types polluter are simultaneously deposited, and polycyclic aromatic hydrocarbon,
The organic pollution of the volatilizations such as organic agricultural chemicals or half volatile increases year by year.Microorganism such as bacterium, microalgae etc. can due to having
Degradable organic pollutant, improve sewage quality, do not generate secondary pollution and it is at low cost the advantages that and cause more and more to pay close attention to,
More and more researchs pay attention to screen specified microorganisms for continuously cultivating and removing polluter using microorganism.Therefore,
In the course of the research, a easy reaction unit for continuously cultivating microorganism is very necessary.
It is a culture solution that can accommodate microorganism and maintenance growth for continuously cultivating micro-biological reaction device
And the device containing the sewage for needing degradation of contaminant matter.It is commonly used in laboratory and continuously cultivates microorganism or microbial degradation
Experiment is that conical flask in conical flask and is positioned in isothermal vibration incubator and is cultivated by microbial inoculant, in this condition
Under, culture solution or degradation solution directly and air communication, in volatile or half volatile pollutant highly volatile to air and cause
Keep measurement result inaccurate, or in the gas such as CO for measuring cultivated Institute of Micro-biology's generation or consumption2Or O2When, connect with air
Logical culture apparatus is difficult to reach measuring requirement, therefore simple and closed reaction unit is of crucial importance.But only meet with
Upper condition is inadequate, in many experimentations, needs periodically to sample from reaction unit, measures microorganism in solution and grows
Pollution degradation substance content, and gas content in measurement device are waited in amount or aqueous solution, and are not examined in general reaction unit
This point is considered, such as patent No. CN2683656Y, entitled a kind of continuous culture experiment device of microorganism, although compared to tradition
Reactor have many advantages, such as that confined condition, construction is succinct, be easy to observation, but still the behaviour of gas-liquid sample very difficult to realize
Make.
Invention content
The present invention is of the existing technology in order to solve the problems, such as, provide it is a kind of can keep confined condition be used for microorganism
Continuous culture or microbial degradation and the reactor assembly of gas-liquid sample can be sampled.By microorganism and its culture medium or dirt
Water is contained in the device, and the device is positioned in isothermal vibration incubator, can be used for continuously cultivating microorganism such as bacterium
With microalgae etc.；Or reaction unit when as sewage of the microbial degradation containing polluter, and due to resilient plug, it can
To provide closed environment, when cultivating microorganism, it is convenient to sample liquid sample and measure microorganism increment, and gas sampled
Sample measures gas componant and content are to determine the metabolic capability of microorganism；It, can be convenient when as degradation experiment reaction unit
Ground sampling liquid sample measures the concentration for waiting for pollution degradation substance, and the polluter such as multiring aromatic hydrocarbon for being easy to volatilization
Organic matter, sampling gas sample and the content for measuring the polluter in gas sample, thus to obtain more real
Test result.The easy device also can be used as the anti-of the chemical experiment that other need confined condition and regular gas-liquid sample to sample simultaneously
Answer device.
To achieve the above object, specific technical solution is as follows：
It is a kind of to be easily used for the closed growth of microorganism continuous and gas-liquid sample sampler, including transparent conical flask, silicon
Glue cork, syringe needle and gas phase rubber spacer；The silica gel cork has elasticity, tight to fill in conical flask bottle mouth position,
The closing for realizing environment in conical flask prevents gas in bottle from being exchanged with air communication；The silica gel cork is equipped with a hole
Road has a diameter larger than microsyringe needle diameter to be connected to environment and outside air in conical flask, for using micro-sampling
Device penetrates extraction gas sample；Gas phase rubber spacer is bonded above duct, is passed through in sampling with the closing of environment in realization
Microsyringe pricks phase rubber spacer of breathing freely and extracts gas sample, and gas-chromatography or other instruments can be used to be analyzed.Silicon
Through syringe needle is inserted on glue cork, syringe needle liquid sucting port is always positioned at liquid level hereinafter, for liquid sampling and guarantee taper
Environment is closed in bottle.
Said syringe syringe needle is stainless steel material.
Above-mentioned silica gel cork diameter should be consistent in conical flask inner diameter size.
Above-mentioned rubber spacer has corrosion-resistant and high-temperature resistant characteristic.
Beneficial effects of the present invention：
1) present invention is using this device culture microorganism or as the anti-of microbial degradation dirt pollutant in water
Container is answered, compared with traditional reaction unit, is capable of providing a kind of closed environmental condition, to measure in incubation or reacting
Produced by journey or the offer of the gas of consumption is possible, while being also possible to prevent volatile or half volatile organic compounds from solution
It overflows and is diffused into outside air, measure the concentration of conical flask gas partial contamination substance, obtain more accurately testing knot
Fruit.
2) gas-liquid sample can be easily measured by sampling in the present invention, and great convenience is provided for experiment.
3) on the basis of realizing above functions, which is positioned in constant temperature illumination box and is cultivated, can adjusted in due course
Required temperature is saved, and the operation of similar stirring is provided, compared with other microorganism reactors, the present invention has structure letter
It is single, be easy to observe, it is at low cost, it is easy to operate the advantages that.
Description of the drawings
The closed growth of Fig. 1 microorganism continuous and gas-liquid sample sampler front view.
The vertical view of the closed growth of Fig. 2 microorganism continuous and gas-liquid sample sampler silica gel cork.
In figure：1 stainless steel syringe syringe needle；2 gas phase rubber spacers；3 silica gel corks；4 conical flasks；5 culture solutions or degradation
Liquid level on sewage.
Specific implementation mode
Embodiment：
It is a kind of to be for the closed growth of microorganism continuous and gas-liquid sample sampler, the main part of the device easily
The transparent conical flask 5 of one 250ml size, the device also includes a diameter and conical flask internal diameter of the bottleneck size phase in addition
The silica gel cork 3 of symbol, a stainless steel syringe syringe needle 1 and a gas phase rubber spacer 2.
Toxic compounds benzene in soil and water environment is discharged into due to the generally existing in fuel and oil product
(B), toluene (T), ethylbenzene (E) and ortho-xylene (X), commonly referred to as BTEX, in this research, we study screening obtain it is efficient
Aerobic degradation of the oil degradation bacterial strain to BTEX.
To realize this process, 100ml is prepared in conical flask 5 and contains the culture medium of object, and by microbial inoculant
In culture medium or sewage containing pollutant.Conical flask soft silica gel plug 3 is sealed and with one layer of tinfoil paper covering to prevent from volatilizing
Property BTEX is attracted on silica gel cork 3.Stainless pin 1 penetrates culture medium liquid level or less and for taking fluid sample.It is inserted into rubber
After glue partition 2, gas sample is sucked using airtight needle.
Ready device is positioned in isothermal vibration incubator and is maintained at 32 DEG C, 150rpm, constant temperature incubation, often
Head space and water phase are sampled and are analyzed respectively within 12 hours.In gas sampled sample, microsyringe can be used to prick ventilative
Phase rubber spacer takes out 2 and takes gas sample, and analysis is detected using gas-chromatography or other instruments；In sampling liquid sample
When, it is installed to stainless steel syringe syringe needle 1 using syringe, extracts required sample size, carry out subsequent detection and analysis.
Claims (1)
1. a kind of easily for the closed growth of microorganism continuous and gas-liquid sample sampler, which is characterized in that including transparent
Conical flask (5), silica gel cork (3), syringe needle (1) and gas phase rubber spacer (2)；The silica gel cork (3) has
Elasticity, tight plug realize the closing of environment in conical flask in conical flask bottle mouth position, prevent gas in bottle from being exchanged with air communication；Institute
The silica gel cork stated is equipped with a duct to be connected to environment and outside air in conical flask, has a diameter larger than microsyringe needle
Head diameter, for penetrating extraction gas sample using microsyringe；Gas phase rubber spacer (2) is bonded above duct, to realize
The closing of interior environment is pricked phase rubber spacer (2) of breathing freely by microsyringe and extracts gas sample in sampling；Silica gel cork
(3) through syringe needle (1) is inserted on, syringe needle liquid sucting port is always positioned at liquid level hereinafter, for liquid sampling and guarantee taper
Environment is closed in bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810466319.1A CN108680395A (en) | 2018-05-09 | 2018-05-09 | It is a kind of to be easily used for the closed growth of microorganism continuous and gas-liquid sample sampler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810466319.1A CN108680395A (en) | 2018-05-09 | 2018-05-09 | It is a kind of to be easily used for the closed growth of microorganism continuous and gas-liquid sample sampler |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108680395A true CN108680395A (en) | 2018-10-19 |
Family
ID=63806544
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810466319.1A Pending CN108680395A (en) | 2018-05-09 | 2018-05-09 | It is a kind of to be easily used for the closed growth of microorganism continuous and gas-liquid sample sampler |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108680395A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109468221A (en) * | 2018-12-14 | 2019-03-15 | 山东科技大学 | A kind of experimental provision of indoor simulation Microbial Carbonates |
| CN111534421A (en) * | 2020-05-14 | 2020-08-14 | 中国科学院沈阳应用生态研究所 | Anaerobic culture device, soil anaerobic culture method and sampling method of anaerobic culture device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201331512Y (en) * | 2008-12-31 | 2009-10-21 | 扬州大学 | Testing device for measuring total discharge amount of N2O gas in oil |
| CN102798557A (en) * | 2012-08-10 | 2012-11-28 | 中国科学院亚热带农业生态研究所 | Method and reactor for real-time online detection of substance nutrient bio-utilization |
-
2018
- 2018-05-09 CN CN201810466319.1A patent/CN108680395A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201331512Y (en) * | 2008-12-31 | 2009-10-21 | 扬州大学 | Testing device for measuring total discharge amount of N2O gas in oil |
| CN102798557A (en) * | 2012-08-10 | 2012-11-28 | 中国科学院亚热带农业生态研究所 | Method and reactor for real-time online detection of substance nutrient bio-utilization |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109468221A (en) * | 2018-12-14 | 2019-03-15 | 山东科技大学 | A kind of experimental provision of indoor simulation Microbial Carbonates |
| CN111534421A (en) * | 2020-05-14 | 2020-08-14 | 中国科学院沈阳应用生态研究所 | Anaerobic culture device, soil anaerobic culture method and sampling method of anaerobic culture device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0124193B1 (en) | Method and device for bacterial testing | |
| CN101131384A (en) | Method for detecting subaqueous acute biological toxicity using photobacteria | |
| CN101130808B (en) | Fast-culturing susceptibility and micro-detection method for mycobacteria and device thereof | |
| CN103045518A (en) | Method for cultivating and detecting mycoplasma pneumoniae | |
| CN108680395A (en) | It is a kind of to be easily used for the closed growth of microorganism continuous and gas-liquid sample sampler | |
| CN200978283Y (en) | Automation bacteria isolated cultivating instrument | |
| JP2008136440A (en) | Syringe-shaped microorganism culture device | |
| US8143019B2 (en) | Portable microbiological testing device for gases | |
| CN207133222U (en) | A kind of culture apparatus for sediment remediation simulated experiment | |
| CN106277364B (en) | The imitative experimental appliance and its experimental method of in-situ immobilization richness nitrogen GEOTHERMAL WATER effect | |
| CN103224885B (en) | A kind of pollutent efficient degradation bacterial screening method cultivated based on microplate | |
| CN107779395A (en) | A kind of high flux micro algae growth test device and method | |
| CN209098683U (en) | A kind of Novel anaerobic fermenting experiment device | |
| CN107446833A (en) | A kind of culture medium and culture tube that can cultivate methane-oxidizing bacteria | |
| CN101619342B (en) | Simple method for detecting water genotoxicity substances in source water | |
| US6605446B2 (en) | Detecting airborne microorganisms | |
| CN204455100U (en) | Bacterial culture sample collecting inoculating bottle | |
| Olson | Modified bottle plate for the cultivation of strict anaerobes | |
| CN201634678U (en) | Small-sized fermentation device simulating environment of intestinal canal | |
| CN109868234A (en) | A kind of polycyclic aromatic hydrocarbon-degrading bacteria and its polycyclic aromatic hydrocarbons contaminated methods and applications of detection | |
| CN201092573Y (en) | Microorganism drug sensitive culturing and testing device | |
| US20230383233A1 (en) | Apparatus for controlled environment establishment, maintenance, and sampling | |
| CN202177616U (en) | Device for measuring carbon dioxide quantity generated during degradation of organic substance by aerobic microorganism | |
| JP7303411B2 (en) | Water quality determination method and water quality determination device using fermentation power of yeast, and experimental device equipped with water quality determination device | |
| CN103421684A (en) | Gas-producing microbe rapid examining tube |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181019 |
|
| WD01 | Invention patent application deemed withdrawn after publication |