CN105154322A - Microbe inoculation device capable of automatically identifying culture dish and identifying method - Google Patents

Microbe inoculation device capable of automatically identifying culture dish and identifying method Download PDF

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Publication number
CN105154322A
CN105154322A CN201510568596.XA CN201510568596A CN105154322A CN 105154322 A CN105154322 A CN 105154322A CN 201510568596 A CN201510568596 A CN 201510568596A CN 105154322 A CN105154322 A CN 105154322A
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China
Prior art keywords
culture dish
identify
automatically
identifying
microbe
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CN201510568596.XA
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Chinese (zh)
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CN105154322B (en
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韩莹莹
鲁颖
聂晖
宁春华
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JINAN BAIBO BIOTECHNOLOGY Co Ltd
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JINAN BAIBO BIOTECHNOLOGY Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention belongs to the technical field of microbe inoculation, and particularly relates to a microbe inoculation device capable of automatically identifying a culture dish and an identifying method. The microbe inoculation device capable of automatically identifying the culture dish comprises the culture dish and an inoculating instrument and is characterized in that the culture dish is of a colorless transparent disposable round plastic structure, the edge of the surface of an upper cover of the culture dish is provided with an image identifier matched with the culture dish, the inoculating instrument is provided with a photograph system, the photograph system is connected with an image identifying processing system, and the image identifying processing system is connected with a data storage system. The device and the method have the beneficial effects that whether the culture dish is a required one can be quickly determined, and a mistake is avoided; the operation is simple, the working efficiency is high, the cost is low, waste is avoided, the accuracy and stability of a test result are guaranteed, the difficult matching problem of an existing automatic microbe inoculating instrument and the culture dish is solved, and the device and the method are suitable for being widely popularized and applied.

Description

A kind of microbe inoculating device and recognition methods that automatically can identify culture dish
(1) technical field
The invention belongs to microbial inoculant technical field, particularly a kind of microbe inoculating device and recognition methods that automatically can identify culture dish.
(2) background technology
Inoculation object microorganism is moved by aseptic technique requirement the process receiving culture medium.At present, microbial inoculant is by manually having come, and there is many defects: the factors such as the randomness of the Different periods of individual level difference, personal habits and same person, affect effect of inoculation, adds the difficulty that doctor judges; Efficiency is low, cost is high, labour intensity is large; Must extreme care during operator's operation, otherwise probably have germ and be stained with to hand or with it splashing, harm produced to HUMAN HEALTH.
Nowadays; automatic vaccination device has been there is for overcoming the above problems on market; but same in actual use existing defects: inoculation instrument is when inoculating; multiple different substratum may be needed; different object microorganisms needs to move to receive in specific substratum; inoculation instrument often there will be the problem of wrong substratum in seeded process, has had a strong impact on effect of inoculation and even can cause irremediable loss.In addition, inoculation instrument and culture dish are all matching used, because be just equipped with corresponding system and function for the feature of its culture dish when inoculating instrument and manufacturing, if arbitrarily change different culture dish, damage will be caused to inoculation instrument, also can affect stability and the accuracy of experimental result; Adopt disposable circular culture dish to be the classical way that country advocates for inoculating the inoculation of instrument, culture dish is water white transparency, is beneficial to the morphological specificity of observing culture, analyzes growing state.If arbitrarily change the culture dish that automatization classification inoculation apparatus matches, the schedule of operation of classification inoculation apparatus setting themselves can be affected, easily cause line error, and cause the phenomenons such as erroneous results.On the other hand, bring great inconvenience also can to clinical manipulation personnel, and be unfavorable for that the equal comparation and assessment between hospital wait work.
(3) summary of the invention
The present invention in order to make up the deficiencies in the prior art, provide a kind of simple to operate, working efficiency is high, reduce costs, avoid wasting, ensureing microbe inoculating device and the recognition methods that automatically can identify culture dish of experimental result accurate stable.
The present invention is achieved through the following technical solutions:
A kind of microbe inoculating device that automatically can identify culture dish, comprise culture dish and inoculation instrument, it is characterized in that: described culture dish is water white disposable round plastic structure, the edge of the upper lid surface of culture dish is provided with the pattern identification matched, inoculation instrument is provided with camera system, camera system is connected with image recognition processing system, and image recognition processing system is connected with data-storage system.
Described data-storage system is connected with computer.
Automatically can identify a recognition methods for culture dish, it is characterized in that: comprise the following steps:
(1), set up required culture dish pattern identification in advance and get lid and operate corresponding database information, the non-required culture dish pattern identification database information corresponding with power operation and store;
(2), to culture dish take pictures, obtain image, and the graphic information obtained is identified, if identify consistent, then instruct and carry out next step and get lid operation; If identify inconsistent, then guidance system carries out power operation.
Preferably, in step (1), in database, input type of culture medium, use date, culture dish accumulation usage quantity and number information.
The invention has the beneficial effects as follows: can automatic decision whether be required substratum rapidly, prevent from makeing mistakes; Simple to operate, working efficiency is high, reduce costs, avoid waste, ensure that experimental result accurate stable, solve the difficult problem that existing automatic microorganism inoculation instrument and culture dish match, be suitable for wide popularization and application.
(4) accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is further illustrated.
Accompanying drawing 1 is structured flowchart of the present invention.
(5) embodiment
Accompanying drawing is a kind of specific embodiment of the present invention.This embodiment comprises culture dish and inoculation instrument, it is characterized in that: described culture dish is water white disposable round plastic structure, the edge of the upper lid surface of culture dish is provided with the pattern identification matched, be easy to the growing state of later observations culture, inoculation instrument is provided with camera system, camera system is connected with image recognition processing system, and image recognition processing system is connected with data-storage system.Data-storage system is connected with computer.
Adopt a kind of microbe inoculating device that automatically can identify culture dish of the present invention, in data-storage system, set up the database information that specificity pattern identification is corresponding with " getting lid to operate " in advance, set up the database information of non-specific pattern identification corresponding " power operation " and store.Camera system being arranged on culture dish uncaps before operation, and shooting culture dish upper cover, obtains image, and is identified the graphic information obtained by image recognition processing system.If identify consistent, then instruct and carry out next step operation and " get lid operation "; If identify inconsistent, then instruct corresponding system " power operation ".Also comprise in database information: type of culture medium, use date, culture dish add up usage quantity and corresponding numbering.Data-storage system is connected with computer, can reads, store, print data library information.

Claims (4)

1. one kind can identify the microbe inoculating device of culture dish automatically, comprise culture dish and inoculation instrument, it is characterized in that: described culture dish is water white disposable round plastic structure, the edge of the upper lid surface of culture dish is provided with the pattern identification matched, inoculation instrument is provided with camera system, camera system is connected with image recognition processing system, and image recognition processing system is connected with data-storage system.
2. a kind of microbe inoculating device that automatically can identify culture dish according to claim 1, is characterized in that: described data-storage system is connected with computer.
3. automatically identify a recognition methods for culture dish according to claim 1 or 2 any one, it is characterized in that: comprise the following steps:
(1), set up required culture dish pattern identification in advance and get lid and operate corresponding database information, the non-required culture dish pattern identification database information corresponding with power operation and store;
(2), to culture dish take pictures, obtain image, and the graphic information obtained is identified, if identify consistent, then instruct and carry out next step and get lid operation; If identify inconsistent, then guidance system carries out power operation.
4. a kind of recognition methods that automatically can identify culture dish according to claim 3, is characterized in that: in step (1), inputs type of culture medium, uses date, culture dish accumulation usage quantity and number information in database.
CN201510568596.XA 2015-09-09 2015-09-09 Microbe inoculation device capable of automatically identifying culture dish and identifying method Active CN105154322B (en)

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CN105154322B CN105154322B (en) 2017-04-19

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107118950A (en) * 2017-05-06 2017-09-01 尹康康 A kind of bacterium automatic vaccination instrument
CN111024967A (en) * 2019-12-19 2020-04-17 济南百博生物技术股份有限公司 Sample identification device and identification method of microorganism pretreatment system
CN112725168A (en) * 2021-01-08 2021-04-30 湖南隋侯珠科技有限公司 Automatic processing system for microbial samples

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CN205046117U (en) * 2015-09-09 2016-02-24 济南百博生物技术股份有限公司 But microorganism of automatic identification culture dish inoculation device

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US20050277169A1 (en) * 2004-05-14 2005-12-15 Sigmund Janet M Active microcosm: screening antimicrobial producing microorganisms
CN201704329U (en) * 2010-06-17 2011-01-12 于大胜 Microorganism incubator on automatic constant touch agar surface
CN102757891A (en) * 2011-04-28 2012-10-31 魏志勇 Automatic microbial inoculation instrument
CN202643695U (en) * 2012-05-31 2013-01-02 徐志全 Culture dish with label
CN203320010U (en) * 2013-02-24 2013-12-04 王敬华 Automatic rapid multi-channel bacterial inoculating instrument
CN104616115A (en) * 2015-02-13 2015-05-13 上海市同济医院 Laboratory microbial culture management system and method and bar code coding method thereof
CN205046117U (en) * 2015-09-09 2016-02-24 济南百博生物技术股份有限公司 But microorganism of automatic identification culture dish inoculation device

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Title
王春玉等: "PREVI Isola自动接种仪的应用评价", 《国际检验医学杂志》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107118950A (en) * 2017-05-06 2017-09-01 尹康康 A kind of bacterium automatic vaccination instrument
CN107118950B (en) * 2017-05-06 2019-10-11 广西梧州圣源茶业有限公司 A kind of bacterium automatic vaccination instrument
CN111024967A (en) * 2019-12-19 2020-04-17 济南百博生物技术股份有限公司 Sample identification device and identification method of microorganism pretreatment system
CN112725168A (en) * 2021-01-08 2021-04-30 湖南隋侯珠科技有限公司 Automatic processing system for microbial samples

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