CN116121047A - Colony counter - Google Patents
Colony counter Download PDFInfo
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- CN116121047A CN116121047A CN202211098616.8A CN202211098616A CN116121047A CN 116121047 A CN116121047 A CN 116121047A CN 202211098616 A CN202211098616 A CN 202211098616A CN 116121047 A CN116121047 A CN 116121047A
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- light source
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the field of biomedical equipment, and discloses a colony counter which comprises a shell, a camera system, a control system and a light source system, wherein the control system is arranged in the shell and is used for man-machine interaction and image analysis, the camera system is arranged in the shell and is positioned above the shell, the light source system is divided into an upper light source and a lower light source, the upper light source is arranged around the camera system, the lower light source is arranged between the control system and the camera system, the upper light source is an annular light band, the lower light source is a lattice light source, and the light source system provides a uniform light source for the camera system. The invention provides a colony counter with double light homogenizing effects, which is automatic equipment for automatically counting colonies based on an image processing technology, and has better efficiency and more convenient calculation compared with manual counting; compared with the existing bacteria counting instrument, the light homogenizing effect is better, and the bacteria counting instrument has the advantages of being high in efficiency, convenient to operate, high in accuracy and the like.
Description
Technical Field
The invention relates to a colony counter, in particular to a colony counter based on an image processing technology, which is particularly suitable for a common nutrient agar pouring instrument for culturing colonies in a culture dish and counting, and belongs to the field of biomedical equipment.
Technical Field
In industries such as agriculture, food, medicine and the like, related detection work is generally required to be carried out on bacteria through biological experiments, the number of bacterial colonies is an important standard for measuring whether food, medicines and the like are qualified or not, and meanwhile, the bacterial colony number is also an important basis for measuring the antibacterial and sterilizing effects of materials in the materialology, so that how to accurately and rapidly determine the number of bacterial colonies becomes a problem to be solved in the industry. The national standard test method, the common nutrient agar pouring method, is usually adopted in China and mainly depends on manual observation and counting, and the method has certain subjectivity, low efficiency and large error and is easy to cause fatigue of staff. With the continuous development of computer and image analysis technology, the working intensity of related personnel is reduced by means of a computer and an image processing tool, the working efficiency is improved, and automatic counting gradually becomes a trend.
Disclosure of Invention
The invention aims to provide a method for solving the problem of manual counting of culture dish colonies, and the traditional manual counting method has the defects of low efficiency, large error, eye fatigue and the like. The invention adopts the image processing scheme to automatically count the colonies on the culture dish, and has the advantages of high efficiency, convenient operation, high accuracy and the like.
The invention is realized by the following scheme: the utility model provides a colony counter, includes casing, control system, camera system and light source system, its characterized in that: the control system is arranged in the shell and used for man-machine interaction and image analysis, the camera system is arranged in the shell and is positioned above the shell, the light source system is divided into an upper light source and a lower light source, the upper light source is arranged around the camera system, the lower light source is arranged between the control system and the camera system, the upper light source is an annular light band, the lower light source is a lattice light source, and the light source system provides a uniform light source for the camera system;
the casing includes: the device comprises a base, a switch door, a front panel, an upper cover plate, a right side plate, a left side plate, a rear cover plate and a partition plate, wherein the base, the switch door, the front panel, the upper cover plate, the right side plate, the left side plate, the rear cover plate and the partition plate are mutually connected through fasteners to form a whole machine body shell;
the control system comprises: the power supply, the starting key, the USB interface, the display interface, the power plug, the radiator and the control main board are arranged in the base and are positioned on one side of the partition board, the starting key is arranged on the front side board of the base, the USB interface, the display interface and the power plug are arranged on one side of the base, and the radiator is arranged on the other side of the base;
the camera system includes: the camera mounting plate is locked on the right side plate and the left side plate through the mounting plate fixing piece, the camera mounting frame is fixed on the camera mounting plate through bolts, and the high-definition camera is mounted on the camera mounting frame;
the light source system includes: the upper light source comprises an annular light source, a lamplight power supply control board and a lamplight power supply control board mounting seat, the lamplight power supply control board is fixed on the lamplight power supply control board mounting seat through a locking screw, and the annular light source is fixed on the lower side surface of the camera mounting plate to provide uniform illumination for the whole camera system; the lower light source comprises a light source mounting plate, a light homogenizing plate and a light homogenizing light source, wherein the light source mounting plate is fixed on the upper part of the base, the light homogenizing light source is fixed in the middle position below the light source mounting plate, and the light homogenizing plate is arranged on the upper side of the light homogenizing light source; when the colony of the culture dish is photographed, the brightness of the upper light source and the lower light source is controlled to realize the regulation and control of the brightness of the colony.
Furthermore, the annular light source is a strip-shaped annular light band, and the periphery of the annular light source is provided with a flexible light guide film. The light homogenizing effect is superior to the existing dome light scheme, and the light path structural design is simpler than the dome light design. In addition, compared with the existing circular LED light source scheme, the circular light source can effectively avoid the problem of hollow and LED lamp bead mapping caused by direct irradiation.
Further, even light source be circular structure, evenly set up 36-64 LED lamp pearls according to the size inside of culture dish, the top is equipped with even light board, can effectively avoid LED lamp pearl effect and the problem that luminance diverged.
Further, a ring structure for placing the culture dish is arranged above the light homogenizing plate and used for marking the placing position of the culture dish.
Compared with the prior art, the invention provides a colony counter with double light homogenizing effects, which is automatic equipment for automatically counting colonies based on an image processing technology. Compared with the existing bacteria counting instrument, the bacteria counting instrument has the advantages of higher manual counting efficiency, more convenient counting and better light homogenizing effect. The counting algorithm adopted by the invention is a contour radian detection algorithm, and is more accurate and higher in calculation efficiency compared with the traditional contour segmentation counting algorithm.
Drawings
Fig. 1 is a schematic diagram of an axial structure according to an embodiment of the present invention.
Fig. 2 is a schematic diagram of an upper cover structure according to an embodiment of the invention.
Fig. 3 is a schematic diagram of a rear internal structure of an embodiment of the present invention.
Fig. 4 is a schematic diagram of the front internal structure of an embodiment of the present invention.
Fig. 5 is a top view of an internal structure of an embodiment of the present invention.
FIG. 6 is a schematic diagram of the lower light source axis.
Fig. 7 is a bottom view of the lower light source.
The figure shows: 1 is a base, 2 is a switch door, 3 is a front panel, 4 is an upper cover plate, 5 is a right side plate, 6 is a left side plate, 7 is a back cover plate, 8 is a power supply, 9 is a partition plate, 10 is a lamplight power supply control panel mounting seat, 11 is a camera mounting plate, 12 is a camera mounting frame, 13 is a high-definition camera, 14 is a mounting plate fixing piece, 15 is a light source mounting plate, 16 is a light homogenizing plate, 17 is a start button, 18 is a USB interface, 19 is a display interface, 20 is a power plug, 21 is a radiator, 22 is a light homogenizing light source, and 23 is an annular light source.
Detailed Description
The following specific examples further describe the present invention, but the embodiments of the present invention are not limited to the following examples:
example 1:
according to the accompanying figures 1 to 7: the utility model provides a colony counter, includes casing, control system, camera system and light source system, its characterized in that: the control system is arranged in the shell and used for man-machine interaction and image analysis, the camera system is arranged in the shell and is positioned above the shell, the light source system is divided into an upper light source and a lower light source, the upper light source is arranged around the camera system, the lower light source is arranged between the control system and the camera system, the upper light source is an annular light band, the lower light source is a lattice light source, and the light source system provides a uniform light source for the camera system;
the casing includes: the base 1, the switch door 2, the front panel 3, the upper cover plate 4, the right side plate 5, the left side plate 6, the rear cover plate 7 and the partition plate 9 are mutually connected through fasteners to form the whole engine body shell;
the control system comprises: the power supply 8, the start-up key 17, the USB interface 18, the display interface 19, the power plug 20, the radiator 21 and the control main board are arranged in the base 1 and are positioned on one side of the partition board 9, the start-up key 17 is arranged on the front side board of the base 1, the USB interface 18, the display interface 19 and the power plug 20 are arranged on one side of the base 1, and the radiator 21 is arranged on the other side of the base 1;
the camera system includes: the camera mounting plate 11 is locked on the right side plate 5 and the left side plate 6 through the mounting plate fixing piece 14, the camera mounting frame 12 is fixed on the camera mounting plate 11 through bolts, and the high-definition camera 13 is mounted on the camera mounting frame 12;
the light source system includes: the upper light source comprises an annular light source (23), a lamplight power supply control board and a lamplight power supply control board mounting seat 10, wherein the lamplight power supply control board is fixed on the lamplight power supply control board mounting seat 10 through a locking screw, the annular light source 23 is fixed on the lower side surface of the camera mounting plate 11, and uniform illumination is provided for the whole camera system; the lower light source comprises a light source mounting plate 15, a light homogenizing plate 16 and a light homogenizing light source 22, wherein the light source mounting plate 15 is fixed on the upper part of the base 1, the light homogenizing light source 22 is fixed in the middle position below the light source mounting plate 15, and the light homogenizing plate 16 is arranged on the upper side of the light homogenizing light source 22; when the colony of the culture dish is photographed, the brightness of the upper light source and the lower light source is controlled to realize the regulation and control of the brightness of the colony.
Example 2:
this embodiment differs from embodiment 1 in that: the annular light source 23 is a strip annular light band, and a flexible light guide film is arranged on the periphery of the annular light band. The light homogenizing effect is superior to the existing dome light scheme, and the light path structural design is simpler than the dome light design. In addition, the ring light source 23 can effectively avoid the problem of hollow and LED bead mapping caused by direct irradiation of the latter, compared with the conventional ring LED light source scheme.
Example 3:
this embodiment differs from embodiment 1 in that: the dodging light source 22 is of a circular structure, 36-64 LED lamp beads are uniformly arranged in the culture dish according to the size of the culture dish, and the dodging plate 16 is arranged above the culture dish, so that the problems of LED lamp bead effect and brightness divergence can be effectively avoided.
Example 4:
this embodiment differs from embodiment 1 in that: the circular ring structure for placing the culture dish is arranged above the light homogenizing plate 16 and is used for marking the placing position of the culture dish.
The working principle of the invention is as follows: firstly, the power is turned on, a culture dish is placed in a circle marked by a light homogenizing plate 16, then the brightness of a light source is adjusted, the brightness parameters of the first adjusted up and down lamplight are recorded by a system, and when the counting is started next time, the system parameters are automatically loaded by colony counting software. After the placing position and the light brightness of the culture dish are regulated, a camera key of colony counting software is clicked, photographing is started, colony images of the culture dish are obtained, then the area for counting the colonies is selected, the fungus counting software supports rectangular area selection, circular area selection, polygonal area selection and the like, automatic counting can be carried out after the area is determined, and after the counting is finished, manual complementation and redundant colony deletion can be carried out if individual counting is inaccurate.
Claims (4)
1. The utility model provides a colony counter, includes casing, control system, camera system and light source system, its characterized in that: the control system is arranged in the shell and used for man-machine interaction and image analysis, the camera system is arranged in the shell and is positioned above the shell, the light source system is divided into an upper light source and a lower light source, the upper light source is arranged around the camera system, the lower light source is arranged between the control system and the camera system, the upper light source is an annular light band, the lower light source is a lattice light source, and the light source system provides a uniform light source for the camera system;
the casing includes: the novel multifunctional machine body comprises a base (1), a switch door (2), a front panel (3), an upper cover plate (4), a right side plate (5), a left side plate (6), a rear cover plate (7) and a partition plate (9), wherein the base (1), the switch door (2), the front panel (3), the upper cover plate (4), the right side plate (5), the left side plate (6), the rear cover plate (7) and the partition plate (9) are mutually connected through fasteners to form a whole machine body shell;
the control system comprises: the power supply (8), a starting key (17), a USB interface (18), a display interface (19), a power plug (20), a radiator (21) and a control main board, wherein the power supply (8) is arranged in the base (1) and is positioned on one side of the partition board (9), the starting key (17) is arranged on the front side board of the base (1), the USB interface (18), the display interface (19) and the power plug (20) are arranged on one side of the base (1), and the radiator (21) is arranged on the other side of the base (1);
the camera system includes: the camera mounting plate (11), the camera mounting frame (12), the high-definition camera (13) and the mounting plate fixing piece (14), wherein the camera mounting plate (11) is locked on the right side plate (5) and the left side plate (6) through the mounting plate fixing piece (14), the camera mounting frame (12) is fixed on the camera mounting plate (11) through bolts, and the high-definition camera (13) is mounted on the camera mounting frame (12);
the light source system includes: the upper light source comprises an annular light source (23), a lamplight power supply control board and a lamplight power supply control board mounting seat (10), wherein the lamplight power supply control board is fixed on the lamplight power supply control board mounting seat (10) through a locking screw, the annular light source (23) is fixed on the lower side surface of the camera mounting plate (11), and uniform illumination is provided for the whole camera system; the lower light source comprises a light source mounting plate (15), a light homogenizing plate (16) and a light homogenizing light source (22), wherein the light source mounting plate (15) is fixed on the upper part of the base (1), the light homogenizing light source (22) is fixed at the middle position below the light source mounting plate (15), and the light homogenizing plate (16) is arranged on the upper side of the light homogenizing light source (22); when the colony of the culture dish is photographed, the brightness of the upper light source and the lower light source is controlled to realize the regulation and control of the brightness of the colony.
2. A colony counter as claimed in claim 1, wherein: the annular light source (23) is a strip-shaped annular light band, and a flexible light guide film is arranged on the periphery of the annular light band.
3. A colony counter as claimed in claim 1, wherein: the dodging light source (22) is of a round structure, 36-64 LED lamp beads are uniformly arranged in the culture dish according to the size of the culture dish, and the dodging plate (16) is arranged above the culture dish, so that the problems of LED lamp bead effect and brightness divergence can be effectively avoided.
4. A colony counter as claimed in claim 1, wherein: the upper part of the light homogenizing plate (16) is provided with a circular ring structure for placing the culture dish and is used for marking the placing position of the culture dish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211098616.8A CN116121047A (en) | 2022-09-08 | 2022-09-08 | Colony counter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211098616.8A CN116121047A (en) | 2022-09-08 | 2022-09-08 | Colony counter |
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| Publication Number | Publication Date |
|---|---|
| CN116121047A true CN116121047A (en) | 2023-05-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211098616.8A Pending CN116121047A (en) | 2022-09-08 | 2022-09-08 | Colony counter |
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| Country | Link |
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| CN (1) | CN116121047A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116908178A (en) * | 2023-09-13 | 2023-10-20 | 吉林农业大学 | Hypha phenotype acquisition device and method |
-
2022
- 2022-09-08 CN CN202211098616.8A patent/CN116121047A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116908178A (en) * | 2023-09-13 | 2023-10-20 | 吉林农业大学 | Hypha phenotype acquisition device and method |
| CN116908178B (en) * | 2023-09-13 | 2024-03-08 | 吉林农业大学 | Hypha phenotype acquisition device and method |
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