CN116121047A - Colony counter - Google Patents

Abstract

The invention belongs to the field of biomedical equipment, and discloses a colony counter which comprises a shell, a camera system, a control system and a light source system, wherein the control system is arranged in the shell and is used for man-machine interaction and image analysis, the camera system is arranged in the shell and is positioned above the shell, the light source system is divided into an upper light source and a lower light source, the upper light source is arranged around the camera system, the lower light source is arranged between the control system and the camera system, the upper light source is an annular light band, the lower light source is a lattice light source, and the light source system provides a uniform light source for the camera system. The invention provides a colony counter with double light homogenizing effects, which is automatic equipment for automatically counting colonies based on an image processing technology, and has better efficiency and more convenient calculation compared with manual counting; compared with the existing bacteria counting instrument, the light homogenizing effect is better, and the bacteria counting instrument has the advantages of being high in efficiency, convenient to operate, high in accuracy and the like.

Description

A colony counter

technical field

The invention relates to a colony counter, especially suitable for pouring ordinary nutrient agar into a petri dish to cultivate and count colonies, in particular to a colony counter based on image processing technology, which belongs to the field of biomedical equipment.

technical background

In agriculture, food, medicine and other industries, it is generally necessary to carry out relevant detection work on bacteria through biological experiments. The number of colonies is an important standard to measure whether food and drugs are qualified, and it is also an important basis for measuring the antibacterial and bactericidal effect of materials in materials science. Accurately and quickly determining the number of bacterial colonies has become an urgent problem to be solved in the industry. The national standard inspection method commonly used in China—ordinary nutrient agar pouring method—mainly relies on manual observation and counting. This method has certain subjectivity, not only has low efficiency and large errors, but also easily causes staff fatigue. With the continuous development of computer and image analysis technology, automatic counting has gradually become a trend with the help of computer and image processing tools to reduce the work intensity of relevant personnel and improve work efficiency.

Contents of the invention

The purpose of the present invention is to provide a solution to the problem of manual counting of culture dish colonies. The traditional manual counting method has disadvantages such as low efficiency, large error, and eye fatigue. The invention adopts an image processing scheme to automatically count the colonies on the culture dish, and has the advantages of high efficiency, convenient operation and high accuracy.

The present invention is realized by the following scheme: a colony counting instrument, including a casing, a control system, a camera system and a light source system, characterized in that: the control system is arranged inside the casing for human-computer interaction and image analysis, and the camera system The light source system is divided into two parts, the upper light source and the lower light source. The upper light source is set around the camera system, and the lower light source is set between the control system and the camera system. The upper light source is a ring light belt, and the lower light source The light source is a dot matrix light source, and the light source system provides a uniform light source for the camera system;

The casing includes: a base, a switch door, a front panel, an upper cover, a right side panel, a left side panel, a rear cover and a partition, the above-mentioned base, a switch door, a front panel, an upper cover, a right side The board, the left side panel, the rear cover and the partition are connected to each other by fasteners to form the whole body shell;

The control system includes: a power supply, a power-on button, a USB interface, a display interface, a power plug, a radiator, and a control board. On the front panel, the USB interface, monitor interface, and power plug are set on one side of the base, and the radiator is set on the other side of the base;

The camera system includes: a camera mounting plate, a camera mounting frame, a high-definition camera, and mounting plate fixing parts. The above-mentioned camera mounting plate is locked on the right side plate and the left side plate through the mounting plate fixing parts, and the camera mounting frame is locked by bolts. Fixed on the camera mounting plate, the high-definition camera is installed on the camera mounting frame;

The light source system includes two parts: an upper light source and a lower light source, wherein the upper light source includes a ring light source, a lighting power control board and a lighting power control board mounting seat, and the lighting power control board is fixed on the lighting power control board mounting seat by locking screws On the top, the ring light source is fixed on the lower side of the camera mounting plate to provide uniform illumination for the entire camera system; the bottom light source includes a light source mounting plate, a uniform light plate, and a uniform light source. The above-mentioned light source mounting plate is fixed on the upper part of the base, and the uniform light source It is fixed in the lower middle position of the light source installation plate, and the uniform light plate is installed on the upper side of the uniform light source; when taking pictures of the culture dish colony, the brightness of the colony can be adjusted by controlling the brightness of the upper light source and the lower light source.

Further, the ring-shaped light source is a strip-shaped ring-shaped light strip with a flexible light-guiding film on the periphery. This uniform light effect is better than the existing dome light solution, and the light path structure design is simpler than the dome light design. In addition, compared with the existing ring-shaped LED light source scheme, the ring light source can effectively avoid the problems of hollow and LED bead mapping caused by the latter due to direct irradiation.

Further, the uniform light source is a circular structure, and 36-64 LED lamp beads are evenly arranged inside according to the size of the culture dish, and a uniform light plate is arranged on the top, which can effectively avoid the problems of LED lamp bead effect and brightness divergence.

Further, a circular structure for placing the petri dish is provided above the homogenizing plate, which is used to mark the placement position of the petri dish.

Compared with the prior art, the present invention provides a colony counting instrument with double uniform light effect, which is an automatic device for automatic colony counting based on image processing technology. Compared with manual counting, it has higher efficiency and more convenient counting. Compared with the existing bacteria counting instrument, it has better uniform light effect. The counting algorithm adopted in the present invention is a contour radian detection algorithm, which is more accurate and more efficient than the traditional contour segmentation counting algorithm.

Description of drawings

Fig. 1 is a schematic view of the axial structure of an embodiment of the present invention.

Fig. 2 is a schematic diagram of the upper cover structure of the embodiment of the present invention.

Fig. 3 is a schematic diagram of the rear internal structure of the embodiment of the present invention.

Fig. 4 is a schematic diagram of the front internal structure of the embodiment of the present invention.

Fig. 5 is a top view of the internal structure of the embodiment of the present invention.

Figure 6 is a schematic diagram of the axonometric view of the lower light source.

Figure 7 is a bottom view of the lower light source.

As shown in the figure: 1 is the base, 2 is the switch door, 3 is the front panel, 4 is the upper cover, 5 is the right side panel, 6 is the left side panel, 7 is the rear cover, 8 is the power supply, 9 is the partition 10 is the lighting power control board mounting seat, 11 is the camera mounting plate, 12 is the camera mounting frame, 13 is the high-definition camera, 14 is the fixing part of the mounting plate, 15 is the light source mounting plate, 16 is the uniform light plate, and 17 is the power button , 18 is a USB interface, 19 is a display interface, 20 is a power plug, 21 is a radiator, 22 is a uniform light source, and 23 is a ring light source.

Detailed ways

The following specific examples further describe the present invention, and the examples can not be repeated here one by one, but the embodiments of the present invention are not therefore limited to the following examples:

Example 1:

According to accompanying drawings 1 to 7: a colony counting instrument, including a casing, a control system, a camera system and a light source system, is characterized in that: the control system is arranged inside the casing for human-computer interaction and image analysis , the camera system is set inside the casing and located on the upper side. The light source system is divided into two parts: an upper light source and a lower light source. The upper light source is set around the camera system, and the lower light source is set between the control system and the camera system. The upper light source is a ring light. The belt and the lower light source are dot matrix light sources, and the light source system provides a uniform light source for the camera system;

The casing includes: a base 1, a switch door 2, a front panel 3, an upper cover 4, a right side panel 5, a left side panel 6, a rear cover 7 and a partition 9, the base 1, the switch door 2 , the front panel 3, the upper cover plate 4, the right side plate 5, the left side plate 6, the rear cover plate 7 and the partition plate 9 are connected to each other by fasteners to form the whole body shell;

The control system includes: a power supply 8, a power button 17, a USB interface 18, a display interface 19, a power plug 20, a heat sink 21 and a control mainboard. On one side, the power-on button 17 is arranged on the front side plate of the base 1, the USB interface 18, the display interface 19, and the power plug 20 are arranged on one side of the base 1, and the radiator 21 is arranged on the other side of the base 1;

The camera system includes: a camera mounting plate 11, a camera mounting frame 12, a high-definition camera 13 and a mounting plate fixture 14, and the above-mentioned camera mounting plate 11 is locked on the right side plate 5 and the left side plate through the mounting plate fixture 14 6, the camera mounting frame 12 is fixed on the camera mounting plate 11 by bolts, and the high-definition camera 13 is installed on the camera mounting frame 12;

The light source system includes two parts: an upper light source and a lower light source, wherein the upper light source includes a ring light source (23), a lighting power control board and a lighting power control board mounting seat 10, and the lighting power control board is fixed on the lighting power supply by locking screws. On the control panel mounting base 10, the ring light source 23 is fixed on the lower side of the camera mounting plate 11 to provide uniform illumination for the entire camera system; the lower light source includes a light source mounting plate 15, a light uniform plate 16, and a uniform light source 22. Plate 15 is fixed on the top of base 1, uniform light source 22 is fixed on the lower middle position of light source mounting plate 15, and uniform light plate 16 is installed on the upper side of uniform light source 22; The brightness of the light source realizes the control of the brightness and darkness of the colony.

Example 2:

The difference between this embodiment and Embodiment 1 is that the ring light source 23 is a strip-shaped ring light strip with a flexible light guide film on the periphery. This uniform light effect is better than the existing dome light solution, and the light path structure design is simpler than the dome light design. In addition, compared with the existing ring-shaped LED light source solution, the ring light source 23 can effectively avoid the hollow and LED bead mapping problems caused by the latter's direct irradiation.

Example 3:

The difference between this embodiment and Embodiment 1 is that the uniform light source 22 is a circular structure, and 36-64 LED lamp beads are evenly arranged inside according to the size of the culture dish, and a uniform light plate 16 is arranged on the top, which can effectively avoid LED The problem of lamp bead effect and brightness divergence.

Example 4:

The difference between this embodiment and Embodiment 1 is that: above the uniform light plate 16, there is a ring structure for placing the culture dish, which is used to mark the placement position of the culture dish.

The working principle of the present invention is as follows: firstly, turn on the power, place the petri dish in the circle marked by the uniform light plate 16, and then adjust the brightness of the light source, the brightness parameters of the upper and lower lights adjusted for the first time will be recorded by the system, and wait until When counting starts for the first time, the system parameters are automatically loaded by the colony counting software. After adjusting the position of the petri dish and the brightness of the light, click the camera button of the colony counting software to start taking pictures and obtain the colony image of the petri dish, and then select the area to be counted. The counting software supports rectangular area selection, circular area selection and Polygonal area selection, etc., can be counted automatically after the area is determined. After the counting is completed, if there are individual counting inaccuracies, operations such as manual supplementation and deletion of redundant colonies can also be performed.

Claims (4)

1. The utility model provides a colony counter, includes casing, control system, camera system and light source system, its characterized in that: the control system is arranged in the shell and used for man-machine interaction and image analysis, the camera system is arranged in the shell and is positioned above the shell, the light source system is divided into an upper light source and a lower light source, the upper light source is arranged around the camera system, the lower light source is arranged between the control system and the camera system, the upper light source is an annular light band, the lower light source is a lattice light source, and the light source system provides a uniform light source for the camera system;

the casing includes: the novel multifunctional machine body comprises a base (1), a switch door (2), a front panel (3), an upper cover plate (4), a right side plate (5), a left side plate (6), a rear cover plate (7) and a partition plate (9), wherein the base (1), the switch door (2), the front panel (3), the upper cover plate (4), the right side plate (5), the left side plate (6), the rear cover plate (7) and the partition plate (9) are mutually connected through fasteners to form a whole machine body shell;

the control system comprises: the power supply (8), a starting key (17), a USB interface (18), a display interface (19), a power plug (20), a radiator (21) and a control main board, wherein the power supply (8) is arranged in the base (1) and is positioned on one side of the partition board (9), the starting key (17) is arranged on the front side board of the base (1), the USB interface (18), the display interface (19) and the power plug (20) are arranged on one side of the base (1), and the radiator (21) is arranged on the other side of the base (1);

the camera system includes: the camera mounting plate (11), the camera mounting frame (12), the high-definition camera (13) and the mounting plate fixing piece (14), wherein the camera mounting plate (11) is locked on the right side plate (5) and the left side plate (6) through the mounting plate fixing piece (14), the camera mounting frame (12) is fixed on the camera mounting plate (11) through bolts, and the high-definition camera (13) is mounted on the camera mounting frame (12);

the light source system includes: the upper light source comprises an annular light source (23), a lamplight power supply control board and a lamplight power supply control board mounting seat (10), wherein the lamplight power supply control board is fixed on the lamplight power supply control board mounting seat (10) through a locking screw, the annular light source (23) is fixed on the lower side surface of the camera mounting plate (11), and uniform illumination is provided for the whole camera system; the lower light source comprises a light source mounting plate (15), a light homogenizing plate (16) and a light homogenizing light source (22), wherein the light source mounting plate (15) is fixed on the upper part of the base (1), the light homogenizing light source (22) is fixed at the middle position below the light source mounting plate (15), and the light homogenizing plate (16) is arranged on the upper side of the light homogenizing light source (22); when the colony of the culture dish is photographed, the brightness of the upper light source and the lower light source is controlled to realize the regulation and control of the brightness of the colony.

2. A colony counter as claimed in claim 1, wherein: the annular light source (23) is a strip-shaped annular light band, and a flexible light guide film is arranged on the periphery of the annular light band.

3. A colony counter as claimed in claim 1, wherein: the dodging light source (22) is of a round structure, 36-64 LED lamp beads are uniformly arranged in the culture dish according to the size of the culture dish, and the dodging plate (16) is arranged above the culture dish, so that the problems of LED lamp bead effect and brightness divergence can be effectively avoided.

4. A colony counter as claimed in claim 1, wherein: the upper part of the light homogenizing plate (16) is provided with a circular ring structure for placing the culture dish and is used for marking the placing position of the culture dish.

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