CN112080416A - Intelligent microbial culture counting system - Google Patents
Intelligent microbial culture counting system Download PDFInfo
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- 238000009629 microbiological culture Methods 0.000 title claims description 10
- 238000004891 communication Methods 0.000 claims abstract description 18
- 238000010438 heat treatment Methods 0.000 claims description 20
- 244000005700 microbiome Species 0.000 claims description 18
- 230000033228 biological regulation Effects 0.000 claims description 17
- 230000017525 heat dissipation Effects 0.000 claims description 17
- 238000001816 cooling Methods 0.000 claims description 14
- 239000002184 metal Substances 0.000 claims description 10
- 239000000758 substrate Substances 0.000 claims description 10
- 238000001514 detection method Methods 0.000 claims description 7
- 238000005192 partition Methods 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 4
- 238000002955 isolation Methods 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 25
- 238000000034 method Methods 0.000 abstract description 10
- 230000000813 microbial effect Effects 0.000 abstract description 10
- 230000008569 process Effects 0.000 abstract description 9
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- 238000010586 diagram Methods 0.000 description 6
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- 239000001963 growth medium Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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Abstract
The invention relates to the technical field of microbial cultivation, in particular to an intelligent microbial cultivation counting system which comprises a plurality of cultivators and an upper computer, wherein the upper computer is connected with one or more cultivators, each incubator comprises a shell, a cultivation bin, a culture dish, an operation panel, a controller, a temperature control device, a shooting device, a power supply module and a communication module, the cultivation bin is arranged in the shell, the culture dish is placed in the cultivation bin, the temperature control device is connected with the cultivation bin, the shooting device is arranged on the cultivation bin, the shooting device shoots images of the culture dish, the operation panel is arranged on the shell, the operation panel, the temperature control device, the shooting device and the communication module are all connected with the controller, the controller is connected with the upper computer through the communication module, and the power supply module supplies power for other elements. The substantial effects of the invention are as follows: the requirement on the environment is reduced, the efficiency of microbial cultivation and counting can be improved by the shooting device, and the function of reversely checking the growth process of the bacterial colony is provided.
Description
Technical Field
The invention relates to the technical field of microbial culture, in particular to an intelligent microbial culture counting system.
Background
At present, laboratories and factories, such as pharmaceutical factories and water supply companies, adopt artificial culture and counting methods when collecting and culturing microorganisms in the environment, so that a large amount of personnel and time are needed to complete the tedious work when collecting and culturing a large amount of microorganisms. The colony culture process needs personnel to track and observe regularly, the number of colonies depends on manual counting, and the accuracy rate of the colony culture depends on subjective judgment. The existing intelligent counting instruments count after the culture is finished, and the colony culture process is completely separated from the intelligent counting. The culture dish is placed in conventional incubator promptly, observes the growth condition of examining the bacterial colony through personnel's timing tracking, counts after waiting to form visible bacterial colony, because of artificial observation's ageing is relatively poor, because of the bacterial colony spreads, overlaps, leads to the decline of count accuracy.
Chinese patent CN206736253U, 12 months 12 days in 2017, a microbial luminous culture counting device, including the culture box, the inside culture dish rack that sets up of culture box, culture dish rack have the panel, and the culture box bottom installation LED light source of culture dish rack below evenly is provided with the count square of a plurality of fretwork on the panel of culture dish rack, and the culture dish is placed on the count square. According to the technical scheme, the number of microorganisms in the culture dish still needs to be observed manually, and the counting efficiency and the accuracy are not high.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the technical problem of lack of an intelligent microorganism culture counter with accurate and reliable counting at present. The intelligent microorganism culture counting system combines the culture and counting of microorganisms into one device, has the advantage of accurate counting, and has the function of reversely checking the growth process of microorganism colonies.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows: the utility model provides an intelligence microbial cultivation count system, includes a plurality of culture apparatus and host computer, one or more culture apparatus of host computer connection, the culture apparatus includes casing, cultivates storehouse, culture dish, operating panel, controller, temperature regulation and control device, shooting device, power module and communication module, cultivate the storehouse and set up in the casing, the culture dish is placed in cultivateing the storehouse, temperature regulation and control device is connected with cultivateing the storehouse, the shooting device is installed on cultivateing the storehouse, and the shooting device shoots the culture dish image, operating panel installs on the casing, operating panel, temperature regulation and control device, shooting device and communication module all are connected with the controller, and the controller passes through communication module and host computer connection, power module is other component power supplies. The cultivation of microorganism can be carried out in cultivateing the storehouse, and the temperature in cultivateing the storehouse is controlled by temperature regulation and control device, through shooting device periodicity shooting culture dish image, returns the host computer and preserves, not only can reverse look over the microorganism bacterial colony growth process, can also realize the automatic counting through the bacterial colony discernment count software of host computer operation, improves counting efficiency and accuracy.
As preferred, the culture storehouse includes culture dish drawer, 3 look LED lamp plates, baffle and riser, installs the spout in the casing, culture dish drawer slidable mounting be in on the spout, it places the frame to have the culture dish that is used for placing the culture dish on the culture dish drawer, 3 look LED lamp plates are installed on the casing and are located culture dish drawer below, the riser is located near culture dish drawer rear portion, riser and casing butt, the baffle just is located culture dish drawer top with casing fixed connection, the front portion and the casing of baffle, riser, culture dish drawer constitute the closed cavity, closed cavity and temperature regulation and control device intercommunication, the shooting device is installed on the baffle, offers the hole that is used for shooting the device to shoot on the baffle, the position in hole corresponds with the culture dish position. When colony color and be shaded and be close, be difficult to the clear colony of distinguishing, 3 look LED lamp plate provides the backlight of different colours, gathers the culture dish image under the different colours, improves the accuracy of colony discernment, and then improves the accuracy of count.
As preferred, the temperature regulation and control device includes airflow pipeline, fin, fan, heating wire, heating switch and radiator fan, the storehouse is cultivateed to airflow pipeline both ends all intercommunication, the fan inserts airflow pipeline, the fin includes base plate and heat radiation fins, the inner wall butt of base plate and casing, the base plate is inlayed on airflow pipeline, heat radiation fins installs on the base plate, radiator fan installs near heat radiation fins, and radiator fan accelerates heat radiation fins's heat dissipation, the heating wire is inlayed in the base plate, the heating wire is connected to power module through heating switch, heating switch is connected with the controller. For cultivateing the storehouse cooling through fin and radiator fan, make the air flow through air flow pipeline and fan, improve the degree of consistency of cultivateing the storehouse internal gas temperature, improve the temperature of cultivateing in the storehouse through the heating wire, reach the effect that the storehouse temperature was cultivateed in control.
Preferably, the shooting device comprises a plurality of cameras and a plurality of camera mounting frames, the camera mounting frames are installed on the partition plates, the positions of the camera mounting frames correspond to the positions of the culture dish placing frames for placing the culture dishes, the cameras are installed on the camera mounting frames and are connected with the controller. Every culture dish corresponds a camera, and convenient reverse observation bacterial colony begins to cultivate the overall process of accomplishing from zero bacterial colony. The camera is above the culture dish, shoots the positive image of culture dish for the discernment and the count of bacterial colony.
Preferably, still include culture dish detection device, culture dish detection device includes a plurality of ultrasonic ranging module and a plurality of pilot lamp, a plurality of ultrasonic ranging module installs respectively on the camera mounting bracket, ultrasonic ranging module detects the hole that is used for shooting on the alignment baffle, ultrasonic ranging module detects its and hole below object's distance, a plurality of the pilot lamp is arranged and is installed on operating panel, a plurality of ultrasonic ranging module and pilot lamp all are connected with the controller. The culture dish detection device indicates which positions in the culture bin have placed the culture dish, and which positions are vacant, so that an operator can find the vacant culture bin quickly when putting in a new culture dish, and the work efficiency is improved.
Preferably, the culture dish comprises a dish bottom, a dish wall and a dish cover, the dish wall is connected with the dish bottom, the top of the dish wall is flush and is abutted against the dish cover, the outer edge of the dish cover is flanged, the flange is basically abutted against the top of the dish wall, and the size of the dish bottom is smaller than that of the dish cover. With the bottom of the ware vertically dish wall, can cause and press close to the bacterial colony that the dish wall grows and appear overlapping on the vertical direction, easily press a bacterial colony count with two bacterial colonies that the vertical direction was arranged, lead to the count inaccurate, the size at the bottom of the ware is less than the ware lid and leads to the dish wall to be necessarily lean out, and the dish wall that leans out can avoid or reduce overlapping on the vertical direction, improves the count accuracy.
Preferably, the device also comprises a side light source, the side light source is arranged in the culture chamber and is connected with the controller, the 3-color LED lamp panel comprises a shell, a semitransparent black PC board, a barrier strip, a semitransparent white PC board, an LED lamp bracket, a plurality of LED annular lamps, a lamp bracket and a bottom board, the shell is plate-shaped, the edge of the shell is provided with a flange, the bottom plate is connected with the flange, the lamp bracket is fixedly connected with the bottom plate, the housing is provided with a plurality of culture dish placing areas, the LED lamp holder is arranged on the bottom plate and is provided with a hollow part for mounting the LED annular light lamp, the positions of the LED annular light lamps are matched with the culture dish placing areas, the translucent white PC board with the LED lighting fixture butt covers the fretwork area of LED lighting fixture, translucent black PC board is connected with translucent white PC board through the parting bead. Translucent white PC board can provide the light color background, when the dark colony of shooting the colour, can clearly shoot the colony image under the illumination of 3 look LED lamp plates. Except can assisting to shoot the darker bacterial colony of colour, more importantly can close 3 look LED lamp plates when some bacterial colony colour is very light, and black translucent PC board can form dark background color this moment, and under dark background color's the support of depositing, the clear image of these light grey bacterial colonies is just shot very easily to the utilization side light source, improves bacterial colony identification count's accuracy. The side light source is arranged in the culture bin.
Preferably, still include camera fixing base, gallows and a plurality of metal helix, gallows and camera mounting bracket fixed connection, the processing of gallows lower part has spherical inner wall, camera fixing base constitutes the ball with gallows sub-unit connection and articulates, and a plurality of metal helix installs between camera fixing base and camera mounting bracket, and the both ends of a plurality of metal helix all are connected with the controller. The shape of helix is the cylinder helix preferentially, has the voltage difference between two pins that controller and helix's both ends are connected and can make the helix shrink, and the mode that adopts control duty cycle can control the contraction length of helix, and the helix is preferably four, distributes along rectangular summit, through the contraction length of controlling four helices, makes the camera can incline 45 degrees to arbitrary direction for shoot the image of the culture dish of 45 degrees of slope. Preferably, the shooting device further comprises a lateral camera, the lateral camera is located on the side of the culture dish, and the lateral camera shoots lateral images of the culture dish for identification and counting of bacterial colonies.
Preferably, the base is installed on the lower portion of the shell, the cover plate is installed on the upper portion of the shell, the support legs are installed at the bottom of the base, aligning holes corresponding to the positions of the support legs are machined in the cover plate, and the aligning holes are blind holes. Preferably, the cover and base are part of a housing.
Preferably, the power supply module further comprises a back plate, the back plate is mounted at the rear part of the shell, the power supply module comprises a power supply interface, a power supply switch and a switching power supply, the switching power supply is mounted on the base and abuts against the vertical plate, the power supply interface and the power supply switch are mounted on the back plate, and the power supply interface is connected with the switching power supply through the power supply switch; and the back plate is provided with a heat dissipation grid hole. The power interface adopts general interface, facilitates the use, and the heat dissipation can be accelerated to the heat dissipation bars hole.
As preferred, still include panel shell, operating panel PCB board and controller mounting panel, panel shell and base fixed connection, the panel shell has to slope to the top to one side, the panel shell has the fretwork, operating panel PCB board is inlayed on the fretwork of panel shell, operating panel installs on operating panel PCB board, the controller mounting panel is installed on the base and is located the air flow pipeline top, the controller is installed on the controller mounting panel. The oblique upper side of the panel shell is inclined to facilitate the use of the operation panel by personnel.
Preferably, the side light source is arranged on the inner side of the panel shell and is positioned above the culture dish drawer. The preferred mounting position can provide better lighting effects and improve the clarity of shooting when only the side light sources are turned on.
Preferably, the communication module comprises a switch, the switch is fixedly mounted on the base through a switch mounting frame, a plurality of communication indicator lamps are further mounted on the back plate and are respectively connected with ports of the switch. Through the switch can be convenient communicate with the host computer, make things convenient for the control of host computer to the device.
Preferably, the intelligent controller further comprises a network interface and a network card, wherein the network interface is installed on the back plate, the network card is installed on the controller installation plate, the network interface is connected with the network card, and the network card is connected with the controller. The network interface can be conveniently networked, and the device is conveniently controlled by the network.
The substantial effects of the invention are as follows: the culture bin is a relatively closed space, and is provided with a temperature regulating device for regulating and controlling the culture temperature of microorganisms, so that the requirement on the environment is reduced, and the application range is expanded; the shooting device can conveniently observe and record the bacterial colony condition in the culture dish, improve the efficiency of microbial culture and counting, provide a function of reversely checking the growth process of the bacterial colony, and greatly facilitate the work of microbial colony culture counting; the shooting device shoots the culture dish in multiple directions, provides images at multiple angles and can improve the accuracy of colony identification and counting; the culture dish drawer is of a push-pull structure, is convenient to place and take out, can place a plurality of culture dishes, and can be conveniently assembled to support monitoring and counting of a large number of culture dishes; the culture dish with the inclined dish wall reduces the overlapping of bacterial colonies and improves the counting accuracy.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment.
Fig. 2 is a schematic structural view of a camera mounting bracket according to an embodiment.
FIG. 3 is a schematic view of the drawer installation of a culture dish according to an embodiment.
FIG. 4 is a schematic diagram of a controller mounting plate installation according to an embodiment.
Fig. 5 is a schematic diagram of a switching power supply according to an embodiment.
FIG. 6 is a schematic diagram of a backplate structure according to an embodiment.
FIG. 7 is a schematic structural diagram of a temperature control device according to an embodiment.
FIG. 8 is a cross-sectional view of a culture dish according to an embodiment.
Fig. 9 is a schematic view of a 3-color LED lamp panel according to an embodiment.
Fig. 10 is a schematic cross-sectional view of a 3-color LED lamp panel according to an embodiment.
Fig. 11 is a schematic view of a mounting structure of a second camera according to the embodiment.
Fig. 12 is a schematic diagram of oblique shooting of a second camera mounting structure according to the embodiment.
Wherein: 1. cover plate, 2, operation panel, 3, culture dish drawer, 4, alignment hole, 5, base, 6, support leg, 7, camera mounting rack, 8, switch mounting rack, 9, switch, 10, partition board, 11, 3-color LED lamp panel, 12, culture dish placing frame, 13, operation panel PCB board, 14, side light source, 15, fan, 16, switching power supply, 17, controller mounting board, 18, cooling fan, 19, cooling fin, 20, communication indicator lamp, 21, network interface, 22, power interface, 23, power switch, 24, heat dissipation grid hole, 25, back board, 26, dish cover, 27, culture dish, 28, culture medium, 29, culture dish placing area, 30, hanger, 31, camera fixing seat, 32, camera, 33, metal spiral line 1101, 1102, semitransparent black PC board, shell, 1103, isolation bar, 1104, semitransparent white PC board, 1105. LED lamp brackets 1106, LED annular lamps 1107, bottom plates 1108 and lamp supports.
Detailed Description
The following provides a more detailed description of the present invention, with reference to the accompanying drawings.
The first embodiment is as follows:
the utility model provides an intelligence microbial cultivation count system, as shown in figure 1, including a plurality of culture apparatus and host computer, host computer connects one or more culture apparatus, the culture apparatus includes the casing, cultivate the storehouse, culture dish 27, operating panel 2, a controller, temperature regulation and control device, shooting device, power module and communication module, it sets up in the casing to cultivate the storehouse, culture dish 27 is placed in cultivateing the storehouse, temperature regulation and control device is connected with cultivateing the storehouse, shooting device installs on cultivateing the storehouse, shooting device shoots culture dish 27 image, operating panel 2 installs on the casing, operating panel 2, temperature regulation and control device, shooting device and communication module all are connected with the controller, the controller passes through communication module and host computer connection, power module is other component power supplies power. As shown in fig. 2, the shooting device includes a plurality of cameras 32 and a plurality of camera mounting brackets 7, the plurality of camera mounting brackets 7 are installed on the partition board 10, the positions of the camera mounting brackets 7 correspond to the positions of the culture dish placing frames 12 for placing the culture dishes 27, the cameras 32 are installed on the camera mounting brackets 7, and the plurality of cameras 32 are all connected with the controller. Each culture dish 27 corresponds to a camera 32, which is convenient for observing the whole process from the beginning of a zero colony to the completion of culture in a reverse direction. A camera 32 is above the culture dish 27 and takes images of the front of the culture dish 27 for identification and counting of colonies.
The culture bin includes culture dish drawer 3, 3 look LED lamp plate 11, baffle 10 and riser, install the spout in the casing, as shown in fig. 3, culture dish drawer 3 slidable mounting is on the spout, culture dish placing frame 12 that has the culture dish that is used for placing culture dish 27 on the culture dish drawer 3, 3 look LED lamp plate 11 is installed on the casing and is located culture dish drawer 3 below, the riser is located near culture dish drawer 3 rear portion, riser and casing butt, baffle 10 and casing fixed connection just are located culture dish drawer 3 top, baffle 10, the riser, the front portion and the casing of culture dish drawer 3 constitute the closed cavity, closed cavity and temperature regulation and control device intercommunication, the shooting device is installed on baffle 10, offer the hole that is used for shooting the device to shoot on the baffle 10, the position in hole corresponds with culture dish 27 positions. Base 5 installs in the casing lower part, and apron 1 installs on casing upper portion, and stabilizer blade 6 is installed to base 5's bottom, and apron 1 processing has the hole 4 that aligns that corresponds with stabilizer blade 6 position, and it is the blind hole to align hole 4. Panel shell and base 5 fixed connection, the panel shell has to the slope of oblique top, and the panel shell has the fretwork, as shown in fig. 4, operating panel PCB 13 inlays on the fretwork of panel shell, and operating panel 2 installs on operating panel PCB 13, and controller mounting panel 17 installs on base 5 and is located the air current pipeline top, and the controller is installed on controller mounting panel 17. The oblique upper side of the panel shell is inclined to facilitate the use of the operation panel 2 by personnel. The cover plate 1 and the base 5 are part of a housing. As shown in fig. 5 and 6, the back plate 25 is installed at the rear of the housing, the power module includes a power interface 22, a power switch 23 and a switching power supply 16, the switching power supply 16 is installed on the base 5 and abuts against the vertical plate, both the power interface 22 and the power switch 23 are installed on the back plate 25, and the power interface 22 is connected with the switching power supply 16 through the power switch 23; the back plate 25 is provided with heat dissipation grid holes 24. The power interface 22 is a universal interface, so that the use is convenient, and the heat dissipation grid holes 24 can accelerate the heat dissipation.
As shown in fig. 7, the temperature control device includes an airflow pipeline, a heat sink 19, a fan 15, a heating wire, a heating switch and a heat dissipation fan 18, two ends of the airflow pipeline are communicated with the culture chamber, the fan 15 is connected into the airflow pipeline, the heat sink 19 includes a substrate and heat dissipation fins, the substrate is abutted against the inner wall of the casing, the substrate is embedded on the airflow pipeline, the heat dissipation fins are installed on the substrate, the heat dissipation fan 18 is installed near the heat dissipation fins, the heat dissipation fan 18 accelerates the heat dissipation of the heat dissipation fins, the heating wire is embedded in the substrate, the heating wire is connected to the power supply module through the heating switch, and the heating. For cultivateing the storehouse cooling through fin 19 and radiator fan 18, make the air flow through air flow pipeline and fan 15, improve the degree of consistency of cultivateing the interior gas temperature of storehouse, improve the temperature of cultivateing in the storehouse through the heating wire, reach the effect that the storehouse temperature was cultivateed in control.
As shown in fig. 8, the culture dish 27 includes a dish bottom, a dish wall and a dish cover 26, the dish wall is connected with the dish bottom, the top of the dish wall is flush and is abutted against the dish cover 26, the outer edge of the dish cover 26 is flanged and is basically abutted against the top of the dish wall, the size of the dish bottom is smaller than that of the dish cover 26, and the culture medium 28 is placed between the dish bottom and the dish wall. With the bottom of the ware vertically dish wall, can cause and press close to the bacterial colony that the dish wall grows and appear overlapping on the vertical direction, easily press a bacterial colony count with two bacterial colonies that the vertical direction was arranged, lead to the count inaccurate, the size at the bottom of the ware is less than the ware lid 26 and leads to the ware wall must be that to lean out, and the ware wall that leans out can avoid or reduce overlapping on the vertical direction, improves the count accuracy. Culture dish detection device includes a plurality of ultrasonic ranging module and a plurality of pilot lamp, and a plurality of ultrasonic ranging module is installed respectively on camera mounting bracket 7, and the hole that is used for the shooting on the ultrasonic ranging module detection alignment baffle 10, the ultrasonic ranging module detect its and the distance of hole below object, and a plurality of pilot lamp is arranged and is installed on operating panel, and a plurality of ultrasonic ranging module and pilot lamp all are connected with the controller.
As shown in fig. 9 and 10, the side light source 14 is installed in the cultivation cabin, the side light source 14 is connected with the controller, the 3-color LED lamp panel 11 includes a housing 1102, a translucent black PC board 1101, a barrier strip 1103, a translucent white PC board 1104, LED lamp holders 1105, a plurality of LED ring light 1106, a lamp support 1108 and a bottom plate 1107, the housing 1102 is plate-shaped, the edge of the housing 1102 is provided with a flange, the bottom plate 1107 is connected with the flange, the lamp support 1108 is fixedly connected with the bottom plate 1107, the housing 1102 is provided with a plurality of cultivation dish placing areas 29, the LED lamp holders 1105 are installed on the bottom plate 1107, the LED lamp holders 1105 are hollowed out for installing the LED ring light 1106, the positions of the LED ring light 1106 are matched with the cultivation dish placing areas 29, the translucent white PC board 1104 is abutted with the LED lamp holders 1105 and covers the hollowed-out areas of the LED lamp holders 1105, and the translucent black PC board 1101 is connected with. In addition to helping to shoot the colonies with darker colors, it is more important that the 3-color LED lamp panel 11 can be turned off when the color of some colonies is very light, and at this time, under the contrast of the black translucent PC board, the clear images of these light gray colonies can be easily shot by using the side light source 14, so as to improve the accuracy of colony identification and counting.
Communication module includes switch 9, and switch 9 passes through switch mounting bracket 8 fixed mounting on base 5, still installs a plurality of communication pilot lamp 20 on the backplate 25, and a plurality of communication pilot lamp 20 is connected with switch 9's port respectively. The network interface 21 is installed on the back plate 25, the network card is installed on the controller installation plate 17, the network interface 21 is connected with the network card, and the network card is connected with the controller.
The beneficial technical effects of this embodiment are: the cultivation storehouse is relative inclosed space, and has temperature regulation and control device regulation and control microorganism and cultivate the temperature, has reduced the requirement to the environment, and the pile up neatly that this device can be convenient is fit for a large amount of microorganism cultivation technical work very much, through the observation that the shooting device can be convenient and the colony condition in the record culture dish 27, even under the pile up neatly, also do not influence the observation and the count of microorganism growth situation. The system supports remote viewing and provides a new working mode. The efficiency of microbial cultivation and count is improved, the function of looking over bacterial colony growth process in the reverse direction is provided, and the work of microbial colony culture count is greatly facilitated. The culture dish drawer 3 is of a push-pull structure, is convenient to place and take out, can place a plurality of culture dishes 27, and can be convenient to assemble and support monitoring and counting of a large number of culture dishes 27. The culture dish 27 adopting the inclined dish wall innovatively reduces the overlapping of bacterial colonies and can improve the counting accuracy.
Example two:
the utility model provides an intelligence microbial cultivation count system, this embodiment is on the basis of embodiment one, further improvement has been carried out to camera device, as shown in FIG. 11, fig. 12, this embodiment still includes camera fixing base 31, gallows 30 and a plurality of metal helix 33, gallows 30 and camera mounting bracket 7 fixed connection, the processing of gallows 30 lower part has spherical inner wall, camera fixing base 31 constitutes ball joint with gallows 30 sub-unit connection, a plurality of metal helix 33 is installed between camera fixing base 31 and camera mounting bracket 7, the both ends of a plurality of metal helix 33 all are connected with the controller. The shape of helix 33 is the cylinder helix preferentially, and it can make helix 33 shrink to have the voltage difference between two pins that controller and helix 33's both ends are connected, and the mode that adopts control duty cycle can control helix 33's shrink length, and helix 33 is preferred four, distributes along rectangular summit, through the shrink length of controlling four helix 33, makes camera 32 can incline 45 degrees to arbitrary direction for shoot the image of the culture dish 27 of 45 degrees of slope. Preferably, the shooting device further comprises a lateral camera 32, the lateral camera 32 is positioned at the side of the culture dish 27, and the lateral camera 32 shoots a lateral image of the culture dish 27 for identification and counting of colonies. The rest of the structure is the same as the first embodiment. Compared with the first embodiment, the photographing device in the present embodiment photographs the culture dish 27 in multiple directions, provides images at multiple angles, such as front, lateral and 45-degree oblique directions, and can improve the accuracy of colony identification and counting.
The above-described embodiments are only preferred embodiments of the present invention, and are not intended to limit the present invention in any way, and other variations and modifications may be made without departing from the spirit of the invention as set forth in the claims.
Claims (10)
1. An intelligent microorganism culture counting system is characterized in that,
including a plurality of culture apparatus and host computer, one or more culture apparatus of host computer connection, the culture apparatus includes the casing, cultivates storehouse, culture dish, operating panel, controller, temperature regulation and control device, shoots device, power module and communication module, cultivate the storehouse and set up in the casing, the culture dish is placed in cultivateing the storehouse, temperature regulation and control device is connected with cultivateing the storehouse, shoot the device and install on cultivateing the storehouse, shoot the device and shoot the culture dish image, operating panel installs on the casing, operating panel, temperature regulation and control device, shooting device and communication module all are connected with the controller, and the controller passes through communication module and is connected with the host computer, power module is other component power supplies.
2. The intelligent microorganism culture counting system of claim 1,
the utility model discloses a cultivation storehouse, including culture dish drawer, 3 look LED lamp plates, baffle and riser, install the spout in the casing, culture dish drawer slidable mounting is in on the spout, it places the frame to have the culture dish that is used for placing the culture dish on the culture dish drawer, 3 look LED lamp plates are installed on the casing and are located culture dish drawer below, the riser is located near culture dish drawer rear portion, riser and casing butt, the baffle just is located culture dish drawer top with casing fixed connection, the front portion and the casing of baffle, riser, culture dish drawer constitute the closed cavity, closed cavity and temperature regulation and control device intercommunication, the shooting device is installed on the baffle, offers the hole that is used for shooting the device to shoot on the baffle, the position in hole corresponds with the culture dish position.
3. An intelligent microbial culture enumeration system according to claim 1 or 2,
the temperature regulation and control device comprises an airflow pipeline, a cooling fin, a fan, a heating wire, a heating switch and a cooling fan, wherein the two ends of the airflow pipeline are communicated with the culture bin, the fan is connected into the airflow pipeline, the cooling fin comprises a substrate and cooling fins, the substrate is abutted against the inner wall of the shell, the substrate is embedded on the airflow pipeline, the cooling fins are installed on the substrate, the cooling fan is installed nearby the cooling fins, the cooling fan accelerates the cooling of the cooling fins, the heating wire is embedded in the substrate, the heating wire is connected to the power supply module through the heating switch, and the heating switch is connected with the controller.
4. The intelligent microorganism culture counting system of claim 2,
the shooting device comprises a plurality of cameras and a plurality of camera mounting frames, the camera mounting frames are installed on the partition boards, the positions of the camera mounting frames correspond to the positions of the culture dish placing frames for placing the culture dishes, the cameras are installed on the camera mounting frames and are connected with the controller.
5. The intelligent microorganism culture counting system of claim 4,
still include culture dish detection device, culture dish detection device includes a plurality of ultrasonic ranging module and a plurality of pilot lamp, a plurality of ultrasonic ranging module installs respectively on the camera mounting bracket, ultrasonic ranging module detects the hole that is used for shooting on the alignment baffle, ultrasonic ranging module detects its and the distance of hole below object, a plurality of the pilot lamp is arranged and is installed on operating panel, a plurality of ultrasonic ranging module and pilot lamp all are connected with the controller.
6. An intelligent microbial culture enumeration system according to claim 1 or 2,
the culture dish comprises a dish bottom, a dish wall and a dish cover, wherein the dish wall is connected with the dish bottom, the top of the dish wall is flush and is abutted to the dish cover, the outer edge of the dish cover is flanged, the flange is basically abutted to the top of the dish wall, and the size of the dish bottom is smaller than that of the dish cover.
7. An intelligent microbial culture enumeration system according to claim 1 or 2,
the LED lamp comprises a shell, a semitransparent black PC board, an isolation strip, a semitransparent white PC board, an LED lamp holder, a plurality of LED annular light lamps, a lamp support and a bottom board, wherein the 3-color LED lamp board comprises the shell, the semitransparent black PC board, the isolation strip, the semitransparent white PC board, the LED lamp holder, the plurality of LED annular light lamps, the lamp support and the bottom board, the shell is in a plate shape, the edge of the shell is provided with a flanging, the bottom board is connected with the flanging, the lamp support is fixedly connected with the bottom board, the shell is provided with a plurality of culture dish placing areas, the LED lamp holder is installed on the bottom board, the LED lamp holder is provided with a hollow part for installing the LED annular light lamps, the LED annular light lamps are matched with the culture dish placing areas in position, the semitransparent white PC board abuts against and covers the hollow part of the LED lamp holder, and the semitransparent black.
8. The intelligent microorganism culture counting system of claim 4,
camera fixing base, gallows and a plurality of metal helix, gallows and camera mounting bracket fixed connection, the processing of gallows lower part has spherical inner wall, camera fixing base and gallows sub-unit connection constitute the ball articulated, and a plurality of metal helix installs between camera fixing base and camera mounting bracket, and the both ends of a plurality of metal helix all are connected with the controller.
9. An intelligent microbial culture enumeration system according to claim 1 or 2,
the power module comprises a power supply interface, a power switch and a switch power supply, the switch power supply is mounted on the shell and is close to the vertical plate, the power supply interface and the power switch are mounted on the back plate, and the power supply interface is connected with the switch power supply through the power switch; and the back plate is provided with a heat dissipation grid hole.
10. An intelligent microbial culture enumeration system according to claim 1 or 2,
still include panel shell, operating panel PCB board and controller mounting panel, panel shell and casing fixed connection, the panel shell has to slope to the top to one side, the panel shell has the fretwork, operating panel PCB board is inlayed on the fretwork of panel shell, operating panel installs on operating panel PCB board, the controller mounting panel is installed on the casing and is located the air flow pipeline top, the controller is installed on the controller mounting panel.
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