CN109777718A - Colony hybridization acquisition system - Google Patents

Colony hybridization acquisition system Download PDF

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Publication number
CN109777718A
CN109777718A CN201910105636.5A CN201910105636A CN109777718A CN 109777718 A CN109777718 A CN 109777718A CN 201910105636 A CN201910105636 A CN 201910105636A CN 109777718 A CN109777718 A CN 109777718A
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China
Prior art keywords
light
colony hybridization
light source
acquisition system
astigmatism
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CN201910105636.5A
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Chinese (zh)
Inventor
吴艳辉
孙万东
刘丰瑞
王杰伟
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Beijing Luqiao Technology Ltd By Share Ltd
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Beijing Luqiao Technology Ltd By Share Ltd
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Priority to CN201910105636.5A priority Critical patent/CN109777718A/en
Publication of CN109777718A publication Critical patent/CN109777718A/en
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides a kind of Colony hybridization acquisition systems.Colony hybridization acquisition system, comprising: cabinet;Pedestal, the bottom of cabinet is arranged in pedestal, and is used for culture dish holding;Image acquisition part, image acquisition part is arranged at the top of the box body, and the camera lens of image acquisition part is towards pedestal, to acquire the image of culture dish bacterium colony;At least one set of sidelight source component, sidelight source component is arranged on the side wall of the box body, to provide sidelight or fluorescent exciting for culture dish, at least one set of sidelight source component has astigmatism portion, astigmatism portion is located on the light path out of the light source of sidelight source component, so that the culture dish on the light uniform fold pedestal behind astigmatism portion.The present invention solves the problems, such as that the Colony hybridization of Colony hybridization acquisition system acquisition in the prior art is unintelligible and fluorescence excitation light source is less.

Description

Colony hybridization acquisition system
Technical field
The present invention relates to Micro biological Tests apparatus fields, in particular to a kind of Colony hybridization acquisition system.
Background technique
In the Micro biological Tests such as food, drug, cosmetics and feed, it is related to total plate count, coliform, large intestine bar Bacterium, lactic acid bacteria etc. count intermediate item, are both needed to carry out a large amount of bacterium colony counting in statistical result.The work master that bacterium colony counts at present It to be completed by the observation of artificial eye, heavy, the dull inefficiency that works, detection poor repeatability, precision are low and original Data can not save.And the shortcomings that bacterium colony counting device then overcomes artificial counting is automated, high degree of automation, working efficiency High, detection accuracy improves, and in addition reduces stronger to the Specialized Quality requirement of testing staff and objectivity.The bacterium colony of high quality Image Acquisition is the basis of subsequent image processing, and the influence to the accuracy of bacterium colony count results is huge, the bacterium colony figure of high quality As including clear, pollution few, uniform illumination of noise of Colony hybridization etc., and these are all closely bound up with the design of light source.State at present That there are uneven illuminations is even for inside and outside bacterium colony counting equipment, and there are reflection and the refraction speck of light in the edge of culture dish, makes culture dish side The bacterium colony of edge is blocked, to influence the accuracy of bacterium colony count results;At present in the standards such as national standard, rower and ISO, use is glimmering Light method realizes that the quickly detection of microorganism is more and more common, but number system almost all both domestic and external uses ordinary white Light source, even if individual plants have the excitation light source of specific wavelength, but wavelength is single, can only carry out fluorimeter to certain class bacterial strain Number, greatly limits its application range.
It can be seen from the above, the Colony hybridization that the acquisition of Colony hybridization acquisition system exists in the prior art is unintelligible and fluorescence swashs The less problem of light emitting source.
Summary of the invention
The main purpose of the present invention is to provide a kind of Colony hybridization acquisition systems, to solve Colony hybridization in the prior art Acquisition system acquisition Colony hybridization is unintelligible and the less problem of fluorescence excitation light source.
To achieve the goals above, according to an aspect of the invention, there is provided a kind of Colony hybridization acquisition system, packet It includes: cabinet;Pedestal, the bottom of cabinet is arranged in pedestal, and is used for culture dish holding;Image acquisition part, image acquisition part setting At the top of the box body, and the camera lens of image acquisition part is towards pedestal, to acquire the image of culture dish;At least one set of side light source group Part, sidelight source component are arranged on the side wall of the box body, to provide sidelight or fluorescent exciting, at least one set of side light source for culture dish Component has astigmatism portion, and astigmatism portion is located on the light path out of the light source of sidelight source component, so that the light behind astigmatism portion The uniformly culture dish on energy covering base.
Further, the sidelight source component with astigmatism portion includes at least two light sources, and the top and bottom in astigmatism portion It is provided with light source, astigmatism portion includes that the first reflector, the first light-transmitting plate and first of the successively side wall setting far from cabinet dissipate Tabula rasa.
Further, astigmatism portion further includes softbox, and softbox is located at side of first astigmatism plate far from the first light-transmitting plate, And softbox is arc panel to be directed away from the direction protrusion of the first astigmatism plate.
Further, the radius of curvature R of softbox is greater than 80mm and is less than 120mm, and the radian Q of softbox is greater than 40 degree And less than 80 degree.
Further, light source is LED light strip.
Further, sidelight source component is two, and two sidelight source components are arranged on one group of opposite side wall of cabinet And on sustained height.
Further, at least one set of sidelight source component can issue the light and/or ultraviolet light of multiple color, wherein a variety of The light of color includes at least white light.
Further, pedestal has back light source, and back light source is irradiated towards the top of cabinet, to illuminate the bottom of culture dish Portion.
Further, Colony hybridization acquisition system further includes filter, and filter is removably disposed in image acquisition part Camera lens on, with to enter camera lens exciting light be filtered.
Further, back light source includes that the second astigmatism plate being successively arranged far from pedestal, the second light-transmitting plate and second are reflective Plate, the circumferential direction of the second light-transmitting plate are provided at least one light source.
Further, Colony hybridization acquisition system further include: control assembly, control assembly at least control image acquisition part Power supply, the power supply of sidelight source component, back light source power supply in one;Processing unit, processing unit receive and process image and adopt The data of collection portion acquisition;One end of multiport transponder, multiport transponder is connect with image acquisition part, multiport transponder The other end is connect with processing unit, the data forwarding that image acquisition part is acquired to processing unit, and control assembly, processing are single The setting of at least one of member, multiport transponder is at the top of the box body.
It applies the technical scheme of the present invention, the Colony hybridization acquisition system in the application includes: cabinet, pedestal, and image is adopted Collection portion and at least one set of sidelight source component.The bottom of cabinet is arranged in pedestal, and for fixing culture dish;Image acquisition part is set It sets at the top of the box body, and the camera lens of image acquisition part is towards pedestal, to acquire the image of culture dish;The setting of sidelight source component exists On the side wall of cabinet, to provide sidelight or fluorescent exciting for culture dish, at least one set of sidelight source component has astigmatism portion, astigmatism Portion is located on the light path out of the light source of sidelight source component, so that the culture on the light uniform fold pedestal behind astigmatism portion Ware.
Using above structure Colony hybridization acquisition system when, the culture dish to be collected containing bacterium colony is placed in cabinet Portion, and culture dish is placed by pedestal.After culture dish placement, side light source and/or back light source component are connected, and pass through Image acquisition part is acquired the Colony hybridization of culture dish.During Image Acquisition, astigmatism portion can produce side light source Raw light is dissipated, and light is scattered, and so as to guarantee that light can be with each corner of uniform irradiation to culture dish, and then is made The collected Colony hybridization of image acquisition part is more complete, is more clear the Colony hybridization of acquisition, effectively improves acquisition The quality of the Colony hybridization arrived;Solve the problems, such as that fluorescence excitation light source is less.
Detailed description of the invention
The accompanying drawings constituting a part of this application is used to provide further understanding of the present invention, and of the invention shows Examples and descriptions thereof are used to explain the present invention for meaning property, does not constitute improper limitations of the present invention.In the accompanying drawings:
Fig. 1 shows the structural schematic diagram of the Colony hybridization acquisition system of a specific embodiment according to the present invention;With And
Fig. 2 shows the concrete structure schematic diagrams of the sidelight source component in Fig. 1;
Wherein, the above drawings include the following reference numerals:
10, cabinet;20, pedestal;21, back light source;30, culture dish;40, sidelight source component;41, astigmatism portion;411, first Reflector;412, the first light-transmitting plate;413, the first astigmatism plate;414, softbox;42, light source;50, control assembly;60, multiport Transponder;70, image acquisition part.
Specific embodiment
It should be noted that in the absence of conflict, the features in the embodiments and the embodiments of the present application can phase Mutually combination.The present invention will be described in detail below with reference to the accompanying drawings and embodiments.
It should be pointed out that unless otherwise specified, all technical and scientific terms used in this application have and the application The normally understood identical meanings of person of an ordinary skill in the technical field.
In the present invention, in the absence of explanation to the contrary, the noun of locality used such as " upper and lower, top, bottom " is usually needle For direction shown in the drawings, or for component itself is on vertical, vertical or gravity direction;Equally Ground for ease of understanding and describes, and " inside and outside " refers to the inside and outside of the profile relative to each component itself, but the above-mentioned noun of locality is not For limiting the present invention.
Colony hybridization in order to solve the acquisition of Colony hybridization acquisition system in the prior art is unintelligible and fluorescence excitation light source Less problem, this application provides a kind of Colony hybridization acquisition systems.
As shown in Figure 1, the Colony hybridization acquisition system in the application includes: cabinet 10, pedestal 20, image acquisition part 70 with And at least one set of sidelight source component 40.The bottom of cabinet 10 is arranged in pedestal 20, and is used for culture dish holding 30;Image acquisition part 70 are arranged in the top of cabinet 10, and the camera lens of image acquisition part 70 is towards pedestal 20, to acquire the image of culture dish 30;Sidelight Source component 40 is arranged on the side wall of cabinet 10, to provide sidelight or fluorescent exciting, at least one set of side light source for culture dish 30 Component 40 has astigmatism portion 41, and astigmatism portion 41 is located on the light path out of the light source 42 of sidelight source component 40, so that by astigmatism The culture dish 30 on light uniform fold pedestal 20 behind portion 41.
Using above structure Colony hybridization acquisition system when, the culture dish 30 to be collected containing bacterium colony is placed in cabinet 10 Inside, and culture dish 30 is placed by pedestal 20.After the placement of culture dish 30, sidelight source component 40 is connected, and pass through Image acquisition part 70 is acquired the Colony hybridization of culture dish 30.During Image Acquisition, astigmatism portion 41 being capable of opposite side The light that light source 42 generates is dissipated, and scatters light, so as to guarantee that light can be with uniform irradiation to each of culture dish 30 Corner, and then keep the collected Colony hybridization of image acquisition part 70 more complete, it is more clear the Colony hybridization of acquisition, effectively Ground improves the quality of collected Colony hybridization;In addition, sidelight source component can issue the light of multiple color and/or ultraviolet Light solves the problems, such as that fluorescence excitation light source is less.
It should be noted that the sidelight in above-mentioned " sidelight or fluorescent exciting " refer to it is opposite with " fluorescent exciting " " non-fluorescence exciting light ", rather than referred to merely from the light being laterally emitted.
As shown in Fig. 2, the sidelight source component 40 with astigmatism portion 41 includes at least two light sources 42, and the top in astigmatism portion 41 Portion and bottom are provided with light source 42, and astigmatism portion 41 includes successively the first reflector 411 of the side wall setting far from cabinet 10, the One light-transmitting plate 412 and the first astigmatism plate 413.It is arranged in this way, the light that light source 42 can be made to issue is irradiated into first thoroughly In tabula rasa 412, and the light scattered backward in the first light-transmitting plate 412 reflexes to the first astigmatism plate by the first reflector 411 413, it can be scattered by the first astigmatism plate 413 by the light of the first astigmatism plate 413, to make light spread around, in turn Can enable light from all directions uniform irradiation culture dish 30 everywhere, guarantee image acquisition part 70 to culture dish 30 into Row Colony hybridization not will form hot spot or light belt when acquiring.
As shown in Fig. 2, astigmatism portion 41 further includes softbox 414, softbox 414 is located at the first astigmatism plate 413 far from first The side of light-transmitting plate 412, and softbox 414 is arc panel to be directed away from the direction protrusion of the first astigmatism plate 413.Pass through setting Softbox 414 can be such that 414 pairs of the softbox light through the first astigmatism plate 413 further scatters, to guarantee the hair of light Effect is dissipated, the clarity of the Colony hybridization of the acquisition of image acquisition part 70 is effectively guaranteed.
Specifically, the radius of curvature R of softbox 414 is greater than 80mm and is less than 120mm, and the radian Q of softbox 414 is greater than 40 degree and less than 80 degree.It is arranged in this way, can guarantees that softbox 414 is in optimum state to the dispersion effect of light.? In the application, the radius of curvature R of softbox 414 is 100mm, and radian Q is 60 degree.
Specifically, in this application the first reflector 411, the first light-transmitting plate 412, the first astigmatism plate 413 and sheen The length of plate 414 is 180mm, and width is 100mm.
In the specific embodiment of the application, light source 42 is LED light strip.It, can be with by selecting LED light strip as light source 42 The size of flexible setting light source 42, to meet different demands, and LED light strip is low in cost, it is possible to reduce whole system is made Valence.
Specifically, sidelight source component 40 is two, and one group of opposite side of cabinet 10 is arranged in two sidelight source components 40 On wall and on sustained height.It is arranged in this way, the exciting light that can be further ensured that light source 42 issues can be uniform It is radiated at culture dish 30 everywhere, the Colony hybridization clarity for acquiring image acquisition part 70 is higher.
Specifically, at least one set of sidelight source component 40 can issue the light and/or ultraviolet light of multiple color, wherein a variety of The light of color includes at least white light.
In this application, due to selecting LED light strip as light source 42, it is possible to which every light bar, which is arranged, two column light of left and right Source 42, a column are containing White LED light source, and another column are containing ultraviolet and each single color exciting light sources.When to common bacterium colony When Image Acquisition, two column White LED light sources up and down are opened;When carrying out fluorescent image acquisition to certain bacterium colony, open corresponding Excitation light source (such as blue light), to be acquired to fluorescent colony image.It is, of course, also possible to which LED light strip is made to issue red light, orange Coloured light, sodium yellow, green light, purple light and ultraviolet light.
In a specific embodiment, White LED light source is arranged is made of 52 (14 groups) White LED light;Single color Light source row is made of ultraviolet and each single colour light source, and every kind of single colour light source is made of 6 (2 groups) LED lights.Wherein single face Coloured light includes single color of light in 7, is red light (615-625nm), orange-colored light (601-606nm), sodium yellow (585- respectively 595nm), green light (515-535nm), blue light (460-470nm), purple light (400-420nm), ultraviolet light (360- 366nm).Every kind of single color of light is used for the fluorescent image acquisition of one or more of bacterial strains, as " food is micro- by GB 4789.38-2012 Biological test escherichia coli counts " in, escherichia coli colony counting method uses ultraviolet light (360nm~366nm) Fluorescent colony counting is carried out, in GB 8538-2016 " the natural mineral water method of inspection ", pseudomonas aeruginosa is also in purple Fluorescent colony counting is carried out under outer light;The bacterium colony dyed by 5 (6)-carboxyl diacetic acid fluorescein (5 (6)-CFDA), in blue light Under excitation, green fluorescence colonies can be formed.Specifically, pedestal 20 has back light source 21, and back light source 21 is towards the top of cabinet 10 Portion's irradiation, to illuminate the bottom of culture dish 30.It is arranged in this way, can makes back light source 21 while illuminating culture dish 30, Light source 42 can also be provided for image acquisition part 70, the Colony hybridization so as to acquire image acquisition part 70 is more clear.
Specifically, back light source 21 includes that the second astigmatism plate being successively arranged far from pedestal 20, the second light-transmitting plate and second are anti- Tabula rasa, the circumferential direction of the second light-transmitting plate are provided at least one light source 42.
Specifically, Colony hybridization acquisition system further includes filter.When image acquisition part 70 acquires fluorescent colony image, Corresponding single color or ultraviolet light are opened as exciting light, while being installed before the camera lens of image acquisition part 70 corresponding Filter filters out exciting light, and the fluorescence for allowing bacterium colony to emit passes through, so as to effectively carry out to fluorescent colony number image Acquisition.
There are two effects for sidelight source component 40, when being acquired to common Colony hybridization, use white light as side light source, warp White light uniform irradiation is more clear the bacterium colony on culture dish on culture dish after crossing astigmatism portion;When progress fluorescent colony image When acquisition, sidelight source component 40 uses excitation light source, opens corresponding single color light source or ultraviolet light as excitation light source, warp Excitation light source uniform irradiation enables the bacterium colony on culture dish to launch fluorescence on culture dish after crossing astigmatism portion, forms fluorescence Bacterium colony.
Specifically, Colony hybridization acquisition system further includes control assembly 50, processing unit and multiport transponder 60.Control Component 50 processed at least control the power supply of image acquisition part 70, the power supply of sidelight source component 40, back light source 21 power supply in one; Processing unit receives and processes the data of the acquisition of image acquisition part 70;One end of multiport transponder 60 and image acquisition part 70 connect It connects, the other end of multiport transponder 60 is connect with processing unit, the data forwarding that image acquisition part 70 is acquired to processing The top of cabinet 10 is arranged in unit, at least one of control assembly 50, processing unit, multiport transponder 60.In this Shen Please in control assembly 50 include: the first controller, second controller and third controller, respectively to image acquisition part 70, side Light source assembly 40 and the power supply in back light source 21 are controlled.And the data of Colony hybridization are collected in image acquisition part 70 Afterwards, processing unit can be forwarded the data to by multiport transponder 60, to make processing unit to culture dish Colony hybridization On bacterium colony carry out count and other analysis.
It can be seen from the above description that the above embodiments of the present invention realized the following chievements:
1, the Colony hybridization that can be more clear reduces the hot spot and light belt occurred in image;
2, fluorescent colony Image Acquisition can be carried out to different strains by issuing different types of exciting light;
3, structure is simple, cheap.
Obviously, above-mentioned the described embodiment is only a part of the embodiment of the present invention, instead of all the embodiments. Based on the embodiments of the present invention, obtained by those of ordinary skill in the art without making creative efforts all Other embodiments should fall within the scope of the present invention.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singular Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet Include " when, indicate existing characteristics, step, work, device, component and/or their combination.
It should be noted that the description and claims of this application and term " first " in above-mentioned attached drawing, " Two " etc. be to be used to distinguish similar objects, without being used to describe a particular order or precedence order.It should be understood that using in this way Data be interchangeable under appropriate circumstances, so that presently filed embodiment described herein can be in addition to illustrating herein Or the sequence other than those of description is implemented.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.

Claims (11)

1. a kind of Colony hybridization acquisition system characterized by comprising
Cabinet (10);
Pedestal (20), the pedestal (20) are arranged in the bottom of the cabinet (10), and are used for culture dish holding (30);
Image acquisition part (70), described image acquisition portion (70) setting is at the top of the cabinet (10), and described image acquires The camera lens in portion (70) is towards the pedestal (20), to acquire the image of the culture dish (30);
At least one set of sidelight source component (40), the sidelight source component (40) are arranged on the side wall of the cabinet (10), with for The culture dish (30) provides sidelight or fluorescent exciting, and at least one set of sidelight source component (40) has astigmatism portion (41), The astigmatism portion (41) is located on the light path out of the light source (42) of the sidelight source component (40), so that by the astigmatism portion (41) culture dish (30) on pedestal (20) described in the light uniform fold after.
2. Colony hybridization acquisition system according to claim 1, which is characterized in that described with the astigmatism portion (41) Sidelight source component (40) includes at least two light sources (42), and is respectively provided at the top and bottom of the astigmatism portion (41) State light source (42), the astigmatism portion (41) include successively far from the cabinet (10) side wall setting the first reflector (411), First light-transmitting plate (412) and the first astigmatism plate (413).
3. Colony hybridization acquisition system according to claim 2, which is characterized in that the astigmatism portion (41) further includes sheen Plate (414), the softbox (414) are located at side of first astigmatism plate (413) far from first light-transmitting plate (412), And the softbox (414) is arc panel to be directed away from the direction protrusion of first astigmatism plate (413).
4. Colony hybridization acquisition system according to claim 3, which is characterized in that the curvature of the softbox (414) half Diameter R is greater than 80mm and is less than 120mm, and the radian Q of the softbox (414) is greater than 40 degree and less than 80 degree.
5. Colony hybridization acquisition system according to claim 2, which is characterized in that the light source (42) is LED light strip.
6. Colony hybridization acquisition system according to any one of claim 1 to 5, which is characterized in that the side light source group Part (40) is two, and two sidelight source components (40) are arranged on one group of opposite side wall of the cabinet (10) and are in On sustained height.
7. Colony hybridization acquisition system according to any one of claim 1 to 5, which is characterized in that described at least one set Sidelight source component (40) can issue the light and/or ultraviolet light of multiple color, wherein the light of the multiple color includes at least white Light.
8. Colony hybridization acquisition system according to any one of claim 1 to 5, which is characterized in that the pedestal (20) With back light source (21), and the back light source (21) is irradiated towards the top of the cabinet (10), to illuminate the culture dish (30) bottom.
9. Colony hybridization acquisition system according to any one of claim 1 to 5, which is characterized in that the Colony hybridization Acquisition system further includes filter, and the filter is removably disposed on the camera lens in described image acquisition portion (70), with right Exciting light into the camera lens is filtered.
10. Colony hybridization acquisition system according to claim 8, which is characterized in that the back light source (21) includes successively The second astigmatism plate, the second light-transmitting plate and the second reflector far from the pedestal (20) setting, the circumferential direction of second light-transmitting plate It is provided at least one described light source (42).
11. Colony hybridization acquisition system according to claim 8, which is characterized in that the Colony hybridization acquisition system is also Include:
Control assembly (50), the control assembly (50) at least control power supply, the side light source in described image acquisition portion (70) The power supply of component (40), the back light source (21) power supply in one;
Processing unit, the processing unit receive and process the data of described image acquisition portion (70) acquisition;
One end of multiport transponder (60), the multiport transponder (60) is connect with described image acquisition portion (70), described The other end of multiport transponder (60) is connect with the processing unit, and the data of described image acquisition portion (70) acquisition are turned It is sent to the processing unit, the control assembly (50), the processing unit, at least one in the multiport transponder (60) Top of a setting in the cabinet (10).
CN201910105636.5A 2019-02-01 2019-02-01 Colony hybridization acquisition system Pending CN109777718A (en)

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CN209854121U (en) * 2019-02-01 2019-12-27 北京陆桥技术股份有限公司 Colony image acquisition system

Cited By (4)

* Cited by examiner, † Cited by third party
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CN110499233A (en) * 2019-08-27 2019-11-26 广东环凯生物科技有限公司 A kind of light-source system, counter and method of counting for colonometer
CN112080416A (en) * 2020-08-26 2020-12-15 浙江泰林医学工程有限公司 Intelligent microbial culture counting system
CN111996110A (en) * 2020-08-27 2020-11-27 迪泰伊诺(天津)科技有限公司 Water quality bacteria detection and analysis device
CN111996110B (en) * 2020-08-27 2023-08-01 迪泰伊诺(天津)科技有限公司 Water quality bacteria detection and analysis device

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