CN209854121U - Colony image acquisition system - Google Patents
Colony image acquisition system Download PDFInfo
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- CN209854121U CN209854121U CN201920183038.5U CN201920183038U CN209854121U CN 209854121 U CN209854121 U CN 209854121U CN 201920183038 U CN201920183038 U CN 201920183038U CN 209854121 U CN209854121 U CN 209854121U
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Abstract
The utility model provides a bacterial colony image acquisition system. A colony image acquisition system comprising: a box body; the base is arranged at the bottom of the box body and used for placing a culture dish; the image acquisition part is arranged at the top of the box body, and a lens of the image acquisition part faces the base so as to acquire an image of the culture dish bacterial colony; the side light source assembly is arranged on the side wall of the box body to provide side light or fluorescence excitation light for the culture dish, and the side light source assembly is provided with a light scattering part which is positioned on a light emitting path of a light source of the side light source assembly so that light passing through the light scattering part uniformly covers the culture dish on the base. The utility model provides an among the prior art bacterial colony image acquisition system gather the bacterial colony image unclear and the less problem of fluorescence excitation light source.
Description
Technical Field
The utility model relates to a microorganism inspection equipment field particularly, relates to a colony image acquisition system.
Background
In the microbiological examination of foods, medicines, cosmetics, feeds and the like, counting items such as total bacterial colony count, coliform bacteria, escherichia coli, lactic acid bacteria and the like are involved, and a large number of bacterial colony counts are required in the statistics of results. At present, the bacterial colony counting work is mainly finished by observing with artificial naked eyes, and the method has the defects of heavy work, low tedious efficiency, poor detection repeatability, low precision, incapability of storing original data and the like. The automatic colony counting device overcomes the defects of manual counting, has high automation degree, high working efficiency and high detection precision, and reduces the professional literacy requirement on detection personnel and has stronger objectivity. The collection of high-quality colony images is the basis of subsequent image processing, the influence on the accuracy of colony counting results is huge, and the high-quality colony images comprise clear colony images, few pollution noise points, uniform illumination and the like, which are closely related to the design of a light source. At present, colony counting equipment at home and abroad has uneven illumination, and bright spots of light reflection and refraction exist at the edge of a culture dish, so that colonies at the edge of the culture dish are shielded, and the accuracy of a colony counting result is influenced; at present, in national standards, international standards, ISO standards and other standards, rapid detection of microorganisms is more and more common by using a fluorescence method, but counting systems at home and abroad almost all adopt a common white light source, even if individual equipment has an excitation light source with a specific wavelength, the wavelength is single, only certain strains can be subjected to fluorescence counting, and the application range of the counting system is greatly limited.
From the above, the colony image collected by the colony image collecting system in the prior art is not clear, and the fluorescence excitation light source is less.
SUMMERY OF THE UTILITY MODEL
A primary object of the present invention is to provide a colony image collection system to solve the problems of the colony image collection system collected in the prior art that the colony image is not clear and the fluorescence excitation light source is less.
In order to achieve the above object, according to an aspect of the present invention, there is provided a colony image collecting system, comprising: a box body; the base is arranged at the bottom of the box body and used for placing a culture dish; the image acquisition part is arranged at the top of the box body, and a lens of the image acquisition part faces the base so as to acquire an image of the culture dish; at least one set of side light source subassembly, side light source subassembly sets up on the lateral wall of box, for the culture dish provides sidelight or fluorescence excitation light, and at least one set of side light source subassembly has light scattering portion, and the light scattering portion is located the light-emitting path of the light source of side light source subassembly to the culture dish on the even ability cover base of light after making the light that passes through light scattering portion.
Further, the side light source assembly with light scattering portion includes two at least light sources, and the top and the bottom of light scattering portion all are provided with the light source, and light scattering portion is including keeping away from first reflector panel, first light-passing board and the first light-scattering board that the lateral wall of box set up in proper order.
Further, the light diffusion part also comprises a light diffusion plate, the light diffusion plate is positioned on one side of the first light diffusion plate, which is far away from the first light transmission plate, and the light diffusion plate is an arc-shaped plate which is convex towards the direction far away from the first light diffusion plate.
Further, the curvature radius R of the light softening plate is larger than 80mm and smaller than 120mm, and the radian Q of the light softening plate is larger than 40 degrees and smaller than 80 degrees.
Further, the light source is a LED strip.
Furthermore, the number of the side light source assemblies is two, and the two side light source assemblies are arranged on one group of opposite side walls of the box body and are at the same height.
Further, at least one set of side light source assemblies can emit multiple colors of light and/or ultraviolet light, wherein the multiple colors of light include at least white light.
Further, the base has a bottom light source, and the bottom light source illuminates toward the top of the case to illuminate the bottom of the culture dish.
Further, colony image acquisition system still includes the filter plate, and the filter plate detachably sets up on the camera lens of image acquisition portion to filter the exciting light that gets into the camera lens.
Further, the bottom light source comprises a second light diffusion plate, a second light transmitting plate and a second light reflecting plate which are sequentially far away from the base, and at least one light source is arranged on the periphery of the second light transmitting plate.
Further, the colony image acquisition system further comprises: the control assembly at least controls one of a power supply of the image acquisition part, a power supply of the side light source assembly and a power supply of the bottom light source; the processing unit receives and processes the data acquired by the image acquisition part; one end of the multi-port transponder is connected with the image acquisition part, the other end of the multi-port transponder is connected with the processing unit so as to forward data acquired by the image acquisition part to the processing unit, and at least one of the control assembly, the processing unit and the multi-port transponder is arranged at the top of the box body.
Use the technical scheme of the utility model, bacterial colony image acquisition system in this application includes: the light source module comprises a box body, a base, an image acquisition part and at least one group of side light source components. The base is arranged at the bottom of the box body and used for fixing the culture dish; the image acquisition part is arranged at the top of the box body, and a lens of the image acquisition part faces the base so as to acquire an image of the culture dish; the side light source assembly is arranged on the side wall of the box body to provide side light or fluorescence excitation light for the culture dish, and the at least one group of side light source assembly is provided with a light scattering part which is positioned on a light emitting path of a light source of the side light source assembly so that light passing through the light scattering part uniformly covers the culture dish on the base.
When the colony image acquisition system with the structure is used, a culture dish containing the colony to be acquired is arranged in the box body, and the culture dish is arranged through the base. And after the culture dish is placed, the side light source and/or the bottom light source component are/is switched on, and the colony image of the culture dish is collected through the image collecting part. In the image acquisition process, the light scattering part can scatter light rays generated by the side light source to scatter the light, so that the light can be ensured to uniformly irradiate all corners of the culture dish, the colony image acquired by the image acquisition part is more complete, the acquired colony image is clearer, and the quality of the acquired colony image is effectively improved; the problem of few fluorescence excitation light sources is solved.
Drawings
The accompanying drawings, which form a part of the present application, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention. In the drawings:
fig. 1 shows a schematic structural view of a colony image acquisition system according to an embodiment of the present invention; and
FIG. 2 is a schematic diagram showing a detailed structure of the side light assembly of FIG. 1;
wherein the figures include the following reference numerals:
10. a box body; 20. a base; 21. a bottom light source; 30. a culture dish; 40. a side light assembly; 41. a light scattering section; 411. a first reflector; 412. a first light-transmitting panel; 413. a first light diffusion sheet; 414. a light softening plate; 42. a light source; 50. a control component; 60. a multi-port transponder; 70. an image acquisition unit.
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
It is noted that, unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
In the present application, where the contrary is not intended, the use of directional words such as "upper, lower, top and bottom" is generally with respect to the orientation shown in the drawings, or with respect to the component itself in the vertical, perpendicular or gravitational direction; likewise, for ease of understanding and description, "inner and outer" refer to the inner and outer relative to the profile of the components themselves, but the above directional words are not intended to limit the invention.
In order to solve the problem that the colony image collected by the colony image collection system in the prior art is not clear and the fluorescence excitation light source is less, the application provides a colony image collection system.
As shown in fig. 1, the colony image acquiring system in the present application includes: the light source device comprises a box body 10, a base 20, an image acquisition part 70 and at least one group of side light source assemblies 40. The base 20 is arranged at the bottom of the box body 10 and is used for placing the culture dish 30; the image acquisition part 70 is arranged at the top of the box body 10, and the lens of the image acquisition part 70 faces the base 20 to acquire the image of the culture dish 30; the side light source assemblies 40 are arranged on the side wall of the box body 10 to provide side light or fluorescence excitation light for the culture dish 30, at least one group of side light source assemblies 40 is provided with a light scattering part 41, and the light scattering part 41 is positioned on the light outgoing path of the light source 42 of the side light source assembly 40, so that the culture dish 30 on the base 20 is uniformly covered by the light passing through the light scattering part 41.
When the colony image collection system with the above structure is used, the culture dish 30 containing the colony to be collected is placed inside the box body 10, and the culture dish 30 is placed through the base 20. After the culture dish 30 is placed, the side light source assembly 40 is turned on, and the colony image of the culture dish 30 is collected by the image collecting part 70. In the process of image acquisition, the light scattering part 41 can scatter the light generated by the side light source 42 to scatter the light, so that the light can be ensured to uniformly irradiate all corners of the culture dish 30, the colony image acquired by the image acquisition part 70 is more complete, the acquired colony image is clearer, and the quality of the acquired colony image is effectively improved; in addition, the side light source assembly can emit light and/or ultraviolet light with various colors, and the problem that the number of fluorescence excitation light sources is small is solved.
In the above-mentioned "side light or fluorescence excitation light", the side light means "non-fluorescence excitation light" opposite to the "fluorescence excitation light", and does not mean light emitted from a lateral direction.
As shown in fig. 2, the side light source assembly 40 having the light diffusion part 41 includes at least two light sources 42, the light sources 42 are disposed on the top and bottom of the light diffusion part 41, and the light diffusion part 41 includes a first light reflection plate 411, a first transparent plate 412 and a first light diffusion plate 413 which are sequentially disposed away from the side wall of the case 10. Through setting up like this, in the light irradiation entering first light-transmitting board 412 that can make light source 42 send, and the light of backscattering is reflected to first diffusion sheet 413 by first reflector 411 in first light-transmitting board 412, the light through first diffusion sheet 413 can be scattered by first diffusion sheet 413, thereby make light to diffusion all around, and then can make light evenly shine everywhere at culture dish 30 from all directions, guarantee that image acquisition portion 70 can not form facula or light band when carrying out colony image acquisition to culture dish 30.
As shown in fig. 2, the light diffuser 41 further includes a light diffuser plate 414, the light diffuser plate 414 is located on a side of the first light diffuser plate 413 away from the first light transmissive plate 412, and the light diffuser plate 414 is an arc-shaped plate protruding in a direction away from the first light diffuser plate 413. Through setting up the board 414 that softens, can make board 414 that softens further scatter to the light that sees through first board 413 that diffuses to guarantee the effect of dispersing of light, guaranteed the definition of the bacterial colony image of image acquisition portion 70 collection effectively.
Specifically, the curvature radius R of the light diffuser plate 414 is greater than 80mm and less than 120mm, and the radian Q of the light diffuser plate 414 is greater than 40 degrees and less than 80 degrees. By such an arrangement, the light diffusion plate 414 can be ensured to have an optimal light diffusion effect. In the present application, the light softening sheet 414 has a radius of curvature R of 100mm and a curvature Q of 60 degrees.
Specifically, the lengths of the first light reflecting plate 411, the first transparent plate 412, the first light diffusing plate 413, and the light softening plate 414 in the present application are all 180mm, and the widths thereof are all 100 mm.
In a particular embodiment of the present application, the light source 42 is a LED strip. Through selecting LED lamp area as light source 42, can set up the size of light source 42 in a flexible way to satisfy different demands, and LED lamp area low cost can reduce entire system's cost.
Specifically, there are two side light assemblies 40, and the two side light assemblies 40 are disposed on a set of opposite side walls of the cabinet 10 and at the same height. By such an arrangement, the excitation light emitted by the light source 42 can be further ensured to be uniformly irradiated on all parts of the culture dish 30, so that the colony image acquired by the image acquisition part 70 has higher definition.
Specifically, at least one set of side light source assemblies 40 can emit multiple colors of light and/or ultraviolet light, wherein the multiple colors of light include at least white light.
In the present application, since LED strips are selected as the light sources 42, it is possible to provide each strip with two left and right columns of light sources 42, one containing white LED light sources and the other containing ultraviolet and each single-color excitation light source. When an ordinary colony image is collected, an upper column and a lower column of white LED light sources are started; when a certain colony is subjected to fluorescent image acquisition, a corresponding excitation light source (such as blue light) is started, so that a fluorescent colony image is acquired. Of course, the LED strip may also emit red, orange, yellow, green, violet and ultraviolet light.
In one specific embodiment, the white LED light source column consists of 52 (14 groups) white LED lights; a column of single color light sources consisting of uv and single color light sources, each single color light source consisting of 6 (2 groups) LED lights. Wherein the single color light comprises 7 single color lights which are red light (615) -625nm), orange light (601) -606nm), yellow light (585) -595nm, green light (515) -535nm), blue light (460-470nm), purple light (400-420nm) and ultraviolet light (360-366 nm). Each single color light is used for collecting fluorescence images of one or more strains, for example, in GB 4789.38-2012 Escherichia coli counting method for food microbiology inspection, the Escherichia coli plate counting method uses ultraviolet light (360 nm-366 nm) to count fluorescence colonies, and in GB 8538-2016 Drinking Natural mineral Water inspection method, Pseudomonas aeruginosa is also used for counting fluorescence colonies under ultraviolet light; colonies stained with 5(6) -carboxydiacetoxyfluorescein (5(6) -CFDA) form green fluorescent colonies when excited with blue light. Specifically, the base 20 has a bottom light source 21, and the bottom light source 21 irradiates toward the top of the case 10 to illuminate the bottom of the culture dish 30. By such an arrangement, the bottom light source 21 can illuminate the culture dish 30 and also provide the light source 42 for the image acquisition part 70, so that the colony image acquired by the image acquisition part 70 can be clearer.
Specifically, the bottom light source 21 includes a second light-diffusing plate, a second light-transmitting plate and a second light-reflecting plate which are sequentially arranged away from the base 20, and at least one light source 42 is arranged in the circumferential direction of the second light-transmitting plate.
Specifically, the colony image acquisition system further comprises a filter plate. When image acquisition portion 70 gathered fluorescence bacterial colony image, opened corresponding single colour or ultraviolet ray as the exciting light, the corresponding filter plate of installation simultaneously before image acquisition portion 70's camera lens filters the exciting light, allows the fluorescence of bacterial colony emission to pass through to can gather fluorescence bacterial colony number image effectively.
The side light source assembly 40 has two functions, when a common colony image is collected, white light is used as a side light source, and the white light is uniformly irradiated on the culture dish after passing through the light-scattering part, so that the colony on the culture dish is clearer; when fluorescence colony image acquisition is carried out, the side light source assembly 40 uses the excitation light source, starts corresponding single color light source or ultraviolet light as the excitation light source, and the excitation light source evenly shines on the culture dish after the light scattering portion, makes the colony on the culture dish can launch fluorescence, forms the fluorescence colony.
Specifically, the colony image acquisition system further includes a control assembly 50, a processing unit, and a multiport transponder 60. The control assembly 50 controls at least one of the power supply of the image collecting part 70, the power supply of the side light source assembly 40 and the power supply of the bottom light source 21; the processing unit receives and processes the data collected by the image collecting part 70; one end of the multi-port repeater 60 is connected to the image acquisition part 70, the other end of the multi-port repeater 60 is connected to the processing unit to forward the data acquired by the image acquisition part 70 to the processing unit, and at least one of the control assembly 50, the processing unit, and the multi-port repeater 60 is disposed on the top of the cabinet 10. The control assembly 50 in this application comprises: the first controller, the second controller and the third controller respectively control the power supplies of the image acquisition part 70, the side light source assembly 40 and the bottom light source 21. And after the image acquisition portion 70 has acquired the data of the colony image, the data can be forwarded to the processing unit via the multiport transponder 60, thereby enabling the processing unit to count and otherwise analyze the colonies on the colony image of the culture dish.
From the above description, it can be seen that the above-mentioned embodiments of the present invention achieve the following technical effects:
1. a clearer colony image can be obtained, and light spots and light bands appearing in the image are reduced;
2. fluorescent colony image collection can be carried out on different strains by emitting different types of exciting light;
3. simple structure and low cost.
It is obvious that the above described embodiments are only some of the embodiments of the present invention, and not all of them. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts shall belong to the protection scope of the present invention.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular is intended to include the plural unless the context clearly dictates otherwise, and it should be further understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of features, steps, operations, devices, components, and/or combinations thereof.
It should be noted that the terms "first," "second," and the like in the description and claims of this application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the application described herein are capable of operation in sequences other than those illustrated or described herein.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (11)
1. A colony image acquisition system, comprising:
a case (10);
the base (20), the said base (20) is set up in the bottom of the said container (10), and is used for placing the Petri dish (30);
the image acquisition part (70), the image acquisition part (70) is arranged at the top of the box body (10), and the lens of the image acquisition part (70) faces the base (20) so as to acquire the image of the culture dish (30);
the side light source assembly (40) is arranged on the side wall of the box body (10) and used for providing side light or fluorescence excitation light for the culture dish (30), and the side light source assembly (40) is provided with a light scattering part (41), and the light scattering part (41) is positioned on the light outgoing path of a light source (42) of the side light source assembly (40), so that the culture dish (30) on the base (20) is uniformly covered by light passing through the light scattering part (41).
2. A colony image collection system according to claim 1, characterized in that the side light source assembly (40) with the light scattering portion (41) comprises at least two light sources (42), and the light scattering portion (41) is provided with the light sources (42) at the top and bottom, and the light scattering portion (41) comprises a first light reflecting plate (411), a first light transmitting plate (412) and a first light scattering plate (413) which are sequentially arranged away from the side wall of the box body (10).
3. A colony image collection system according to claim 2, wherein the light diffuser (41) further comprises a light diffuser plate (414), the light diffuser plate (414) is located on a side of the first light diffuser plate (413) remote from the first light transmissive plate (412), and the light diffuser plate (414) is an arc-shaped plate protruding in a direction away from the first light diffuser plate (413).
4. Colony image acquisition system according to claim 3, characterized in that the radius of curvature R of the light diffuser plate (414) is larger than 80mm and smaller than 120mm, and the radian Q of the light diffuser plate (414) is larger than 40 degrees and smaller than 80 degrees.
5. Colony image acquisition system according to claim 2, characterized in that said light source (42) is a LED strip.
6. Colony image acquisition system according to any one of claims 1-5, characterized in that the side light assemblies (40) are two, the two side light assemblies (40) being arranged on a set of opposite side walls of the box (10) and at the same height.
7. Colony image acquisition system according to any of claims 1-5, characterized in that at least one group of said side light assemblies (40) is capable of emitting light of multiple colors and/or ultraviolet light, wherein said light of multiple colors comprises at least white light.
8. Colony image acquisition system according to any one of claims 1-5, characterized in that the base (20) has a bottom light source (21), and that the bottom light source (21) is illuminated towards the top of the box (10) to illuminate the bottom of the culture dish (30).
9. Colony image acquisition system according to any of claims 1-5, characterized in that it further comprises a filter plate detachably arranged on the lens of the image acquisition part (70) to filter the excitation light entering the lens.
10. Colony image acquisition system according to claim 8, characterized in that, the bottom light source (21) includes the second scattered light board, the second light-transmitting board and the second reflector panel that set up far away from the base (20) in proper order, the second light-transmitting board circumference be provided with at least one the light source (42).
11. The colony image capturing system of claim 8, further comprising:
a control assembly (50), the control assembly (50) controlling at least one of the power supply of the image acquisition part (70), the power supply of the side light source assembly (40), and the power supply of the bottom light source (21);
a processing unit that receives and processes data acquired by the image acquisition part (70);
a multi-port repeater (60), one end of the multi-port repeater (60) is connected with the image acquisition part (70), the other end of the multi-port repeater (60) is connected with the processing unit so as to forward the data acquired by the image acquisition part (70) to the processing unit, and at least one of the control component (50), the processing unit and the multi-port repeater (60) is arranged on the top of the cabinet (10).
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| CN201920183038.5U CN209854121U (en) | 2019-02-01 | 2019-02-01 | Colony image acquisition system |
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| CN201920183038.5U CN209854121U (en) | 2019-02-01 | 2019-02-01 | Colony image acquisition system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109777718A (en) * | 2019-02-01 | 2019-05-21 | 北京陆桥技术股份有限公司 | Colony hybridization acquisition system |
| CN111269815A (en) * | 2020-02-12 | 2020-06-12 | 贵州理工学院 | Bacteria calculation image acquisition device |
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2019
- 2019-02-01 CN CN201920183038.5U patent/CN209854121U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109777718A (en) * | 2019-02-01 | 2019-05-21 | 北京陆桥技术股份有限公司 | Colony hybridization acquisition system |
| CN111269815A (en) * | 2020-02-12 | 2020-06-12 | 贵州理工学院 | Bacteria calculation image acquisition device |
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