CN101916029B - High-performance illuminator for fluorescence photography - Google Patents
High-performance illuminator for fluorescence photography Download PDFInfo
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- CN101916029B CN101916029B CN2010102383306A CN201010238330A CN101916029B CN 101916029 B CN101916029 B CN 101916029B CN 2010102383306 A CN2010102383306 A CN 2010102383306A CN 201010238330 A CN201010238330 A CN 201010238330A CN 101916029 B CN101916029 B CN 101916029B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/062—LED's
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- General Physics & Mathematics (AREA)
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention relates to a high-performance illuminator for fluorescence photography, in particular to a high-performance illuminator applied to fluorescence photography of biological sample gel. The illuminator mainly comprises a base, a supporting zone and at least a light extraction module, wherein the supporting zone is arranged in the middle zone of the upper surface of the base and is used for supporting a biological sample gel; each light extraction module is arranged on the periphery above the base and comprises a light-emitting diode array which can be provided with various colors of light-emitting diodes to be suitable for different biological reagents; and exciting light is projected from the side of the gel. The illuminator can prevent the bright spot of the illuminator from interfering with fluorescence photography or observation besides greatly reducing the device volume.
Description
Technical field
The present invention is about a kind of high-performance illuminator for fluorescence photography device, particularly about a kind of photofluorographic high-effect fluorescence photography device of biological sample colloid that is applied to.
Background technology
In giving birth to the skill experiment, for all eucaryotes, by the phosphorylation of protein, can make body change intracellular protein activity, change the activity of ferment or use and transmit signal, regulate cellular metabolism, growth, propagation and canceration or the like physiology course.But generally speaking, phosphorylating protein content is extremely low, and normal in vivo journey mobile equilibrium, without the pre-concentration step, often can't effectively detect or carry out biological test.
Develop the west from people such as Towbin in 1979 and change stain method protein such as (Western blot) or nucleic acid (DNA (deoxyribonucleic acid) for example; DNA) after the analytical technology, the west is changeed the stain method and is become extremely welcome analytical approach.The superior analytic ability of stain method association colloid electrophoresis (gel electrophoresis) is changeed in the west, and the technology of the characteristics such as susceptibility of the selectivity of antibody and ferment (enzyme), can in the very complicated compound of composition, a particular proteins or DNA be separated.Sample after utilizing colloid electrophoresis to separate after video picture is handled, can carry out qualitative or quantitative observation again.
And according to the characteristic of sample, employed reagent or stain difference in colour developing or videograph process, the view mode of its sample after video picture is also different.For example, in the observation of DNA fluoroscopic image, the blue light-emitting diode (LED) that need to use wavelength 465nm is as back light, uses the dna sample that excites after the video picture and sends fluorescence, re-uses optical lens and video camera and carries out that shadow is observed or shooting.
See also Fig. 1, be the diagrammatic cross-section of existing fluorescein photographic equipment.As shown in the figure, existing fluorescein photographic equipment 10 consists predominantly of a camera module 12, one amber optical filter (amber filter) 141 and one light source module 18.
Wherein, light source module 18 includes housing 181, and an opening is established in housing 181 tops, and is embedded a blue color filter 187 in opening.The opening below then is provided with a blue light-emitting diode array 183.The DNA colloid 16 of having finished colloid electrophoresis can be positioned on the blue color filter 187.
Utilize blue light-emitting diode array 183 to provide blue light, and, make the light of specific band pass through with blue color filter 187 filtering parasitic lights as excitation source.Send fluorescence by the DNA in the blue-light excited DNA colloid 16 of wavelength 465nm, observe or take fluoroscopic image with camera module 12 again.
For strengthening contrast effect, can set up an amber optical filter 141 in camera module 12 and 16 in DNA colloid, use the filtering back light.In addition, for preventing LED source bright spot interference images or testing result, need a diffusion sheet 185 is set between light emitting diode matrix 183 and blue color filter 187, make pointolite be diffused in the bigger area and become comparatively uniform light source.
Though above-mentioned existing fluorescein photographic equipment can reach the effect of PF and observation, yet because of its constructional restriction, a device can only be applicable to the application of single wavelength excitation source.And the intensity that the setting of diffusion sheet 185 will the loss excitation source in its light source causes the waste of the energy.
Above-mentioned light source module 18 is a backlight arrangement (trans-illuminator), and the fluorescein photographic equipment that other has a light supply apparatus in a kind of use (epi-illuminator) as shown in Figure 2.
As shown in the figure, this fluorescein photographic equipment 20 includes: camera module 22, one amber optical filter 24, an and light source module 26.
Wherein, this light source module 26 includes a housing 269, and housing 269 tops are provided with an opening 261.Housing 269 bottoms are provided with a black substrate 265, and the DNA colloid 28 that desire is observed can be positioned on the black substrate 265 of these opening 261 belows.
Blue-ray LED array 263 is arranged in the zone outside the housing 269 inner and upper openings 261, with irradiation DNA colloid 28 under the oblique side direction, uses exciting DNA colloid 28 to produce fluorescence.
The fluorescein photographic equipment 20 of this pattern owing to blue-ray LED array 263 shines, and utilizes black substrate 265 to absorb most spuious blue light downwards, can prevent the shooting or the observation of light source bright spot interference fluoroscopic image.The amber optical filter 24 filtering blue lights of arranging in pairs or groups again can contrast preferable PF imaging.
Yet, the problem of consideration irradiation average degree and light source and DNA colloid 28 distances, its blue-ray LED array 263 needs to use many row's blue-ray LEDs, and need improve the distribution density of LED, just can obtain preferable radiation response.And, because the relation that structure is provided with forms a large amount of waste irradiation districts 267 in housing 269 inner meetings.So, not only cost of manufacture is high, also can produce a large amount of energy dissipations during use.
In addition, above-mentioned two kinds of existing fluorescein photographic equipments because of the mechanism of its use and irradiation principle need suitable distance just can obtain uniform radiation response, so its device has sizable volume, can't dwindle.
Summary of the invention
Fundamental purpose of the present invention is to provide a kind of high-performance illuminator for fluorescence photography device, refers to a kind of photofluorographic high-effect fluorescence photography device of biological sample colloid that is applied to especially.
Another object of the present invention is to provide a kind of high-performance illuminator for fluorescence photography device, and it is mainly by the side of biological sample colloid projection exciting light, can prevent that the light source bright spot from disturbing, and the person that need not to use the diffusion sheet.
Another purpose of the present invention is to provide a kind of high-performance illuminator for fluorescence photography device, and it goes out optical module and uses the one dimension light emitting diode matrix, can reduce cost of manufacture and reduction means volume person.
Another purpose of the present invention is to provide a kind of high-performance illuminator for fluorescence photography device, and the light emitting diode of multiple coloured light can be set in its light emitting diode matrix, and using provides the excitation source of different demands person.
Another purpose of the present invention is to provide a kind of high-performance illuminator for fluorescence photography device, includes a controller and connects each light emitting diode matrix, in order to control the open and close of light-emitting diode of all kinds.
Another purpose of the present invention is to provide a kind of high-performance illuminator for fluorescence photography device, and its controller still can be adjusted its brightness when light emitting diode is opened.
Another purpose of the present invention is to provide a kind of high-performance illuminator for fluorescence photography device, utilizes concentration piece with the exciting light polymerization or be collimated to biological sample colloid, can improve the utilization rate person of the energy.
Another purpose of the present invention is to provide a kind of high-performance illuminator for fluorescence photography device, and its concentration piece has micro-prism structure, in order to polymerization or collimation exciting light person.
For reaching above-mentioned purpose, the invention provides a kind of high-performance illuminator for fluorescence photography device, include: a base; One supporting region is positioned at the middle section of this base upper surface, in order to carry a biological sample colloid; And at least onely go out optical module, be arranged at the periphery of base upper surface, in order to throw an exciting light to this biological sample colloid.
Description of drawings
Fig. 1 is the diagrammatic cross-section of an existing fluorescein photographic equipment;
Fig. 2 is the diagrammatic cross-section of another existing fluorescein photographic equipment;
Fig. 3 is the diagrammatic cross-section of a preferred embodiment of the present invention;
Fig. 4 is the longitudinal profile synoptic diagram that goes out optical module of the present invention;
Fig. 5 is the schematic cross section that goes out optical module of the present invention.
Wherein, Reference numeral:
10 fluorescein photographic equipments
12 camera modules
141 amber optical filter 16 DNA colloids
18 light source modules, 181 housings
183 blue-ray LED arrays, 185 diffusion sheets
187 blue color filters
20 fluorescein photographic equipments, 22 camera modules
24 amber optical filter 26 light source modules
261 openings, 263 blue-ray LED arrays
265 black substrates, 267 waste irradiation districts
269 housings, 28 DNA colloids
30 high-performance illuminator for fluorescence photography devices
301 camera modules, 303 optical filters
32 bases, 321 upper surfaces
323 supporting regions, 34 biological sample colloid
36 go out optical module 361 light emitting diode matrixs
363 first concentration pieces, 365 second concentration pieces
367 light-emitting windows, 369 controllers
42 horizontal micro-prism structures
52 blue light-emitting diodes, 54 green light LEDs
56 ultraviolet light-emitting diodes, 58 vertical micro-prism structures
Embodiment
See also Fig. 3, be the diagrammatic cross-section of a preferred embodiment of the present invention.As shown in the figure, high-performance illuminator for fluorescence photography device 30 of the present invention includes a base 32, a supporting region 323 and at least onely goes out optical module 36.
Wherein, this supporting region 323 is located at the middle section of base 32 upper surfaces 321, goes out the periphery that optical module 36 is located at base 32 upper surfaces, can be in order to the biological sample colloid in the supporting region 323 34 is throwed exciting lights.Utilization can prevent the shooting or the observation of light source bright spot interference fluoroscopic image by the side-irradiation exciting light of biological sample colloid 34.
When base 32 is circle, goes out optical module 36 and can be the periphery that a doughnut structure is located at base 32 upper surfaces 321.When base 32 when being square, two groups of peripheries that go out optical module 36 in base 32 relative both sides can be set, or four groups of peripheries that go out optical module 36 in each limit are set.
Respectively go out to include a light emitting diode matrix 361 in the optical module 36 respectively, and offer a light-emitting window 367 in a side of contiguous supporting region 323.Light-emitting window 367 can directly be an opening, also can be embedded as transparent panels such as glass or acrylic plates, various coloured light of tolerable or ultraviolet light by and by side-irradiation biological sample colloid 34, the not only biological specimen of applicable different types and stain or reagent, and excitation source does not need through the absorption of spreading layer by layer and filtering, and also can significantly improve for the efficient of energy source use.
Because high-performance illuminator for fluorescence photography device 30 of the present invention is little because of the colloid general thickness by the side projection exciting light of biological sample colloid 34, uses the one dimension light emitting diode matrix can reach good excitation light irradiation effect.
This one dimension light emitting diode matrix 361 can use the light emitting diode of single colored light, use as special purpose, the light emitting diode of multiple coloured light also can be set, the for example blue light-emitting diode among Fig. 5 52, green light LED 54 and ultraviolet light-emitting diodes 56 can be as multi-purpose light supply apparatuses.
In one embodiment of this invention, still can set up a controller 369, connect each light emitting diode matrix 361 respectively, use the unlatching of each light emitting diode of control or close, or control the unlatching of light-emitting diode of all kinds (52,54,56) respectively or close.And can utilize this controller 24 when each light emitting diode is opened, control its luminous brightness respectively.
Utilize high-performance illuminator for fluorescence photography device 30 of the present invention, cooperate camera module 301 and suitable optical filter 303, can use same light supply apparatus to finish the PF or the observation of various biological sample colloid 34, for example: protein colloid, DNA colloid, RNA colloid and polysaccharide (polysaccharide) colloid or the like.
See also Fig. 4, be the longitudinal profile synoptic diagram that goes out optical module of the present invention.Of the present inventionly go out optical module 36 and still can include one first concentration piece 363, be located between light emitting diode matrix 361 and the light-emitting window 367, after light polymerization or collimation that light emitting diode is up dispersed down, be projected to the side of biological sample colloid 34 by light-emitting window 367.This first concentration piece 363 can be provided with a plurality of horizontal microprisms (micro-prismatic structure) structure 42, can reach the spotlight effect of above-below direction.
See also Fig. 5, be the schematic cross section that goes out optical module of the present invention.As shown in the figure, of the present inventionly go out optical module 36 and still can include one second concentration piece 365, be located between light emitting diode matrix 361 and the light-emitting window 367, after light polymerization or collimation that light emitting diode is dispersed toward about, be projected to biological sample colloid 34 by light-emitting window 367.This second concentration piece 365 can be provided with a plurality of vertical micro-prism structures 58, can reach the spotlight effect of left and right directions.
In sum, use high-performance illuminator for fluorescence photography device 30 of the present invention, not only can improve the service efficiency of the energy, and facility there are multiple use, can accomplish the saving and the high efficiency utilization of goods and materials.And, owing to throw exciting light by the side of biological sample colloid 34, and use the one dimension light emitting diode matrix, the volume of high-performance illuminator for fluorescence photography device 30 of the present invention can significantly dwindle, and reaches the light effect that does not take up space.
The above; only be embodiments of the invention; be not to be used for limiting scope of the invention process, all equalizations of doing according to the described shape of claim protection domain of the present invention, structure, feature, method and spirit change and revise, and all should be included in the claim protection domain of the present invention.
Claims (7)
1. a high-performance illuminator for fluorescence photography device is characterized in that, includes:
One base;
One supporting region is positioned at the middle section of this base upper surface, in order to carry a biological sample colloid; And
At least onely go out optical module, be arranged at the periphery of base upper surface, in order to throw an exciting light to the side of this biological sample colloid; Respectively go out optical module and include a light-emitting window, a light emitting diode matrix and at least one concentration piece respectively, this light-emitting window is opened in a side of contiguous supporting region, this light emitting diode matrix includes a plurality of light emitting diodes, respectively in order to required exciting light to be provided, and be projected to this biological sample colloid by this light-emitting window, each concentration piece has a plurality of micro-prism structures respectively, is located between this light emitting diode matrix and the light-emitting window, in order to the exciting light polymerization or be collimated to this biological sample colloid.
2. device as claimed in claim 1 is characterized in that, this light emitting diode matrix is an one dimension light emitting diode matrix.
3. device as claimed in claim 2 is characterized in that, each light emitting diode matrix includes at least a coloured light light emitting diode respectively.
4. device as claimed in claim 3 is characterized in that, this at least a coloured light light emitting diode include blue light-emitting diode, green light LED, ultraviolet light-emitting diodes and knockdown one of them.
5. device as claimed in claim 3 is characterized in that, still includes a controller, connects each light emitting diode matrix, in order to the unlatching of controlling light-emitting diode of all kinds and close.
6. device as claimed in claim 5 is characterized in that, the brightness when this controller still can be adjusted light-emitting diode of all kinds and opens.
7. device as claimed in claim 1 is characterized in that, this biological sample colloid comprises one of them of a protein colloid, a DNA colloid, a RNA colloid and a polysaccharide colloid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102383306A CN101916029B (en) | 2010-07-26 | 2010-07-26 | High-performance illuminator for fluorescence photography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102383306A CN101916029B (en) | 2010-07-26 | 2010-07-26 | High-performance illuminator for fluorescence photography |
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| Publication Number | Publication Date |
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| CN101916029A CN101916029A (en) | 2010-12-15 |
| CN101916029B true CN101916029B (en) | 2011-12-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010102383306A Active CN101916029B (en) | 2010-07-26 | 2010-07-26 | High-performance illuminator for fluorescence photography |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101893809B (en) * | 2010-06-30 | 2012-07-25 | 亚亚科技股份有限公司 | Light source device for fluorescence photography |
| CN103245611A (en) * | 2012-02-07 | 2013-08-14 | 鸿林堂科技股份有限公司 | Multiple excitation light source system |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1036639A (en) * | 1988-02-24 | 1989-10-25 | 株式会社日立制作所 | Fluorescent monitoring electrophoresis apparatus |
| CN2560943Y (en) * | 2002-08-27 | 2003-07-16 | 郭晏海 | Gel image analyser |
| CN1595105A (en) * | 2004-07-04 | 2005-03-16 | 华中科技大学 | Integrated minisize optical analyser |
| CN1595115A (en) * | 2003-09-10 | 2005-03-16 | 开物科技股份有限公司 | Imaging type biological chip instrument |
| CN2831112Y (en) * | 2005-04-18 | 2006-10-25 | 宁波唯奥基因科技发展有限公司 | Electrophoresis separation and analysis device integrated with changeable light source |
| JP2007333479A (en) * | 2006-06-13 | 2007-12-27 | Tokyo Univ Of Science | Fluorescent transilluminator |
| CN101724683A (en) * | 2008-09-23 | 2010-06-09 | 米利波尔公司 | Device for microbiological analysis |
| CN101893809A (en) * | 2010-06-30 | 2010-11-24 | 亚亚科技股份有限公司 | Light source device for fluorescence photography |
-
2010
- 2010-07-26 CN CN2010102383306A patent/CN101916029B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1036639A (en) * | 1988-02-24 | 1989-10-25 | 株式会社日立制作所 | Fluorescent monitoring electrophoresis apparatus |
| CN2560943Y (en) * | 2002-08-27 | 2003-07-16 | 郭晏海 | Gel image analyser |
| CN1595115A (en) * | 2003-09-10 | 2005-03-16 | 开物科技股份有限公司 | Imaging type biological chip instrument |
| CN1595105A (en) * | 2004-07-04 | 2005-03-16 | 华中科技大学 | Integrated minisize optical analyser |
| CN2831112Y (en) * | 2005-04-18 | 2006-10-25 | 宁波唯奥基因科技发展有限公司 | Electrophoresis separation and analysis device integrated with changeable light source |
| JP2007333479A (en) * | 2006-06-13 | 2007-12-27 | Tokyo Univ Of Science | Fluorescent transilluminator |
| CN101724683A (en) * | 2008-09-23 | 2010-06-09 | 米利波尔公司 | Device for microbiological analysis |
| CN101893809A (en) * | 2010-06-30 | 2010-11-24 | 亚亚科技股份有限公司 | Light source device for fluorescence photography |
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| CN101916029A (en) | 2010-12-15 |
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