CN101893809B - Light source device for fluorescence photography - Google Patents
Light source device for fluorescence photography Download PDFInfo
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- CN101893809B CN101893809B CN2010102192474A CN201010219247A CN101893809B CN 101893809 B CN101893809 B CN 101893809B CN 2010102192474 A CN2010102192474 A CN 2010102192474A CN 201010219247 A CN201010219247 A CN 201010219247A CN 101893809 B CN101893809 B CN 101893809B
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- light
- emitting diode
- supply apparatus
- light emitting
- colloid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/062—LED's
- G01N2201/0627—Use of several LED's for spectral resolution
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a light source device for fluorescence photography, in particular relates to a light source device applied to biological sample colloid fluorescence photography. The light source device mainly comprises a shell, a transparent plate and at least one light-emitting diode array, wherein the transparent plate is arranged in a photic zone of the shell; each light-emitting diode array is arranged in a zone beyond the coverage of the photic zone; and a detection result can be prevented from being interfered by the bright spots of the light source through inclined lateral irradiation. Colored light-emitting diodes can be arranged in each light-emitting diode array; and a controller controls the opening and closing of each colored light-emitting diode. The light source device can be applied to various biological reagents.
Description
Technical field
The present invention relates to a kind of photofluorographic light supply apparatus, relate in particular to a kind of photofluorographic light supply apparatus of biological sample colloid that is applied to.
Background technology
In giving birth to the skill experiment; As far as all eucaryotes; Through the phosphorylation of protein, can make body change intracellular protein activity, change the activity of ferment or so as to transmitting signal, regulating cellular metabolism, growth, propagation and canceration or the like physiology course.But generally speaking, phosphorylating protein content is extremely low, and normal in vivo journey mobile equilibrium, without the pre-concentration step, often can't effectively detect or carry out biological test.
Develop the west from people such as Towbin in 1979 and change stain method protein such as (Western blot) or nucleic acid (DNA for example; DNA) after the analytical technology, the west is changeed the stain method and is become extremely welcome analytical approach.It is the superior analytic ability of association colloid electrophoresis (gel electrophoresis) that the stain method is changeed in the west; And the technology of the characteristics such as susceptibility of the selectivity of antibody and ferment (enzyme); Can in the very complicated compound of composition, a particular proteins or DNA be separated.Sample after utilizing colloid electrophoresis to separate after video picture is handled, can carry out qualitative or quantitative observation again.
And according to the characteristic of sample, employed reagent or stain are different in colour developing or videograph process, and the view mode of its sample after video picture is also different.For example, in the observation of DNA fluoroscopic image, the blue light-emitting diode (LED) that need to use wavelength 465nm is as back light, sends fluorescence so as to exciting the dna sample after the video picture, re-uses optical lens and video camera and carries out that shadow is observed or shooting.
See also Fig. 1, it is the diagrammatic cross-section of existing fluorescein photographic equipment.As shown in the figure, existing fluorescein photographic equipment 10 consists predominantly of a camera module 12, one amber optical filter (amber filter) 34 and one light source module 18.
Wherein, light source module 18 includes housing 181, and an opening is established in housing 181 tops, and in opening, is embedded a blue color filter 187.The opening below then is provided with a blue light-emitting diode array 183.The DNA colloid 16 of having accomplished colloid electrophoresis can be positioned on the blue color filter 187.
Utilize blue light-emitting diode array 183 to provide blue light, and, make the light of specific band pass through with blue color filter 187 filtering parasitic lights as excitation source.DNA through in the blue-light excited DNA colloid 16 of wavelength 465nm sends fluorescence, observes or take fluoroscopic image with camera module 12 again.
For strengthening contrast effect, can set up an amber optical filter 141 in camera module 12 and 16 in DNA colloid, so as to the filtering back light.In addition, for preventing LED source bright spot interference images or testing result, need a diffusion sheet 185 is set between light emitting diode matrix 183 and blue color filter 187, make pointolite be diffused in the bigger area and become comparatively uniform light source.
Though above-mentioned existing fluorescein photographic equipment can reach the effect of PF and observation, yet because of its constructional restriction, a device can only be applicable to the application of single wavelength excitation source.And the intensity that the setting of diffusion sheet 185 will the loss excitation source in its light source causes the waste of the energy.
Summary of the invention
Fundamental purpose of the present invention is to provide a kind of photofluorographic light supply apparatus, relates in particular to a kind of photofluorographic light supply apparatus of biological sample colloid that is applied to.
Another object of the present invention is to provide a kind of photofluorographic light supply apparatus, it mainly is that at least one light emitting diode matrix is arranged at outside the photic zone covering scope, prevents that with the irradiation of oblique side direction bright spot from disturbing, and the person that need not to use the diffusion sheet.
Another purpose of the present invention is to provide a kind of photofluorographic light supply apparatus, and its photic zone uses transparent panel, can make multiple excitation source pass through.
Another purpose of the present invention is to provide a kind of photofluorographic light supply apparatus, and the light emitting diode of multiple coloured light can be set in its light emitting diode matrix, so as to the excitation source person of different demands is provided.
Another purpose of the present invention is to provide a kind of photofluorographic light supply apparatus, includes a controller and connects each light emitting diode matrix, in order to control the open and close of light-emitting diode of all kinds.
Another purpose of the present invention is to provide a kind of photofluorographic light supply apparatus, and its controller still can be adjusted its brightness when light emitting diode is opened.
Another purpose of the present invention is to provide a kind of photofluorographic light supply apparatus, and wherein each light emitting diode matrix can be reached different backlight illumination effects through adjusting preset angle horizontal by a preset angle setting.
For reaching above-mentioned purpose, the present invention provides a kind of photofluorographic light supply apparatus, includes: a housing, and the housing top offers a photic zone; One transparent panel is located at this photic zone, in order to carry a biological sample colloid; And at least one light emitting diode matrix, being arranged at the zone outside the photic zone institute covering scope in the housing, each light emitting diode matrix includes a plurality of light emitting diodes.
Wherein, this transparent panel may be selected to be one of them of a glass plate and an acrylic block.
Wherein, each light emitting diode matrix is preset the angle setting with surface level with one respectively.
Wherein, this preset angle can be adjusted on demand.
Wherein, each light emitting diode matrix includes at least a coloured light light emitting diode respectively.
Wherein, this at least a coloured light light emitting diode include blue light-emitting diode, green light LED, ultraviolet light-emitting diodes and knockdown one of them.
Wherein, also include a controller, connect each light emitting diode matrix, in order to the unlatching of controlling light-emitting diode of all kinds and close.
Brightness when wherein, this controller can be adjusted light-emitting diode of all kinds and opens.
Wherein, this controller can be adjusted this preset angle.
Wherein, this biological sample colloid is chosen as one of them of a protein colloid, a DNA colloid, a RNA colloid and a polysaccharide colloid.
Describe the present invention below in conjunction with accompanying drawing and specific embodiment, but not as to qualification of the present invention.
Description of drawings
Fig. 1 is the diagrammatic cross-section of existing fluorescein photographic equipment.
Fig. 2 is the diagrammatic cross-section of the present invention's one preferred embodiment.
Wherein, Reference numeral:
10: fluorescein photographic equipment 12: camera module
141: amber optical filter 16:DNA colloid
18: light source module 181: housing
183: blue-ray LED array 185: diffusion sheet
187: blue color filter
20: photofluorographic light supply apparatus 22: housing
221: transparent panel 223: photic zone
The 26:LED array
261-269: each coloured light LED
28: angle
32: camera module 34: optical filter
36: biological sample colloid
Embodiment
See also Fig. 2, it is the diagrammatic cross-section of the present invention's one preferred embodiment.As shown in Figure 2, the photofluorographic light supply apparatus 20 of the present invention includes a housing 22, a transparent panel 221 and at least one light emitting diode matrix 26.
Wherein, these housing 22 tops offer a photic zone 223, and transparent panel 221 then is arranged at this photic zone 223, can be in order to carry a biological sample colloid 36.Light emitting diode matrix 26 is arranged at outside housing 22 inner photic zone 223 covering scopes, can prevent the shooting or the observation of light source bright spot interference fluoroscopic image through oblique side direction irradiation.
The photofluorographic light supply apparatus 20 of the present invention is provided with transparent panel 221 in photic zone 223; Various coloured light of tolerable or ultraviolet light pass through and direct irradiation biological sample colloid 36; The not only biological specimen of applicable different types and stain or reagent; And excitation source does not need through the absorption of spreading layer by layer and filtering, and can significantly improve for the efficient of energy source use yet.This transparent panel 221 may be selected to be a glass plate or an acrylic plate wherein it
Light emitting diode matrix 26 of the present invention can use the light emitting diode of single colored light; Use as special purpose; The light emitting diode of multiple coloured light also can be set; For example blue light-emitting diode, green light LED and ultraviolet light-emitting diodes (like light-emitting diodes of all kinds such as 261 to 269) are as multi-purpose light supply apparatus.In housing 22, one group of light emitting diode matrix 26 can be set, by the projection of a side wherein excitation source.Two groups of light emitting diode matrixs 26 also can be set, by both sides projection excitation source, obtain radiation response uniformly respectively.Also more multi-group light-emitting diode array 26 can be set on demand, by more direction projection excitation source, so as to reaching the radiation response of demand.
Above-mentioned each light emitting diode matrix 26 can be provided with horizontal by a preset angle 28 respectively.Should still can adjust by preset angle 28 according to the effect of penetrating demand.
In one embodiment of this invention, still can set up a controller 24, connect each light emitting diode matrix 26 respectively, so as to the unlatching of controlling each light emitting diode or close, or control the unlatching of light-emitting diode of all kinds (261 to 269) respectively or close.And this controller 24 capable of using is controlled its luminous brightness respectively when each light emitting diode is opened.In addition, also can be via the preset angle 28 of these controller 24 each light emitting diode matrixs 26 of adjustment.
Utilize photofluorographic light supply apparatus 20 of the present invention; Cooperate camera module 32 and suitable optical filter 34; Can use same light supply apparatus to accomplish the PF or the observation of various biological sample colloid 36, for example: protein colloid, DNA colloid, RNA colloid and polysaccharide (polysaccharide) colloid or the like.
Therefore, use photofluorographic light supply apparatus 20 of the present invention, not only can improve the service efficiency of the energy, and facility there are multiple use, can accomplish the saving and the high efficiency utilization of goods and materials.
Certainly; The present invention also can have other various embodiments; Under the situation that does not deviate from spirit of the present invention and essence thereof; Those of ordinary skill in the art can make various corresponding changes and distortion according to the present invention, but these corresponding changes and distortion all should belong to the protection domain of claim of the present invention.
Claims (10)
1. a photofluorographic light supply apparatus is characterized in that, includes:
One housing, the housing top offers a photic zone;
One transparent panel is located at this photic zone, in order to carry a biological sample colloid; And
At least one light emitting diode matrix is arranged at the zone outside the photic zone institute covering scope in the housing, and the oblique side direction of exciting light that makes each light emitting diode matrix produce exposes to this photic zone, and each light emitting diode matrix includes a plurality of light emitting diodes.
2. light supply apparatus according to claim 1 is characterized in that, this transparent panel may be selected to be one of them of a glass plate and an acrylic block.
3. light supply apparatus according to claim 1 is characterized in that, each light emitting diode matrix is preset the angle setting with surface level with one respectively.
4. light supply apparatus according to claim 3 is characterized in that, this preset angle can be adjusted on demand.
5. light supply apparatus according to claim 4 is characterized in that, each light emitting diode matrix includes at least a coloured light light emitting diode respectively.
6. light supply apparatus according to claim 5 is characterized in that, this at least a coloured light light emitting diode include blue light-emitting diode, green light LED, ultraviolet light-emitting diodes and knockdown one of them.
7. light supply apparatus according to claim 5 is characterized in that, also includes a controller, connects each light emitting diode matrix, in order to the unlatching of controlling light-emitting diode of all kinds and close.
8. light supply apparatus according to claim 7 is characterized in that, the brightness when this controller can be adjusted light-emitting diode of all kinds and opens.
9. light supply apparatus according to claim 7 is characterized in that, this controller can be adjusted this preset angle.
10. light supply apparatus according to claim 1 is characterized in that, this biological sample colloid is chosen as one of them of a protein colloid, a DNA colloid, a RNA colloid and a polysaccharide colloid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102192474A CN101893809B (en) | 2010-06-30 | 2010-06-30 | Light source device for fluorescence photography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102192474A CN101893809B (en) | 2010-06-30 | 2010-06-30 | Light source device for fluorescence photography |
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| Publication Number | Publication Date |
|---|---|
| CN101893809A CN101893809A (en) | 2010-11-24 |
| CN101893809B true CN101893809B (en) | 2012-07-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102192474A Active CN101893809B (en) | 2010-06-30 | 2010-06-30 | Light source device for fluorescence photography |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101916029B (en) * | 2010-07-26 | 2011-12-28 | 亚亚科技股份有限公司 | High-performance illuminator for fluorescence photography |
| CN103245611A (en) * | 2012-02-07 | 2013-08-14 | 鸿林堂科技股份有限公司 | Multiple excitation light source system |
| CN103245640B (en) * | 2012-02-08 | 2015-09-30 | 鸿林堂科技股份有限公司 | Two luminous source lamp box experimental system |
| KR20220033957A (en) * | 2019-07-19 | 2022-03-17 | 어드밴스드 인스트루먼트 피티이. 엘티디. | Optical system, and method of illuminating the sample plane |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1234114A (en) * | 1996-08-16 | 1999-11-03 | 图像研究公司 | Digital imaging system for assays in well plate, gels and blots |
| CN1362621A (en) * | 2000-12-26 | 2002-08-07 | 光荣工业株式会社 | Ultravoilet light/fluorescent light detection apparatus and sensing method thereof |
| CN2560943Y (en) * | 2002-08-27 | 2003-07-16 | 郭晏海 | Gel image analyser |
| CN1595115A (en) * | 2003-09-10 | 2005-03-16 | 开物科技股份有限公司 | Imaging type biological chip instrument |
| CN1595105A (en) * | 2004-07-04 | 2005-03-16 | 华中科技大学 | Integrated minisize optical analyser |
| CN1786689A (en) * | 2004-12-10 | 2006-06-14 | 国际商业机器公司 | Automated inspection system and method |
| CN101724683A (en) * | 2008-09-23 | 2010-06-09 | 米利波尔公司 | Device for microbiological analysis |
| CN201653906U (en) * | 2010-04-17 | 2010-11-24 | 上海交通大学 | Biological immunochromatography chip detector |
| CN101916029A (en) * | 2010-07-26 | 2010-12-15 | 亚亚科技股份有限公司 | High-performance illuminator for fluorescence photography |
-
2010
- 2010-06-30 CN CN2010102192474A patent/CN101893809B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1234114A (en) * | 1996-08-16 | 1999-11-03 | 图像研究公司 | Digital imaging system for assays in well plate, gels and blots |
| CN1362621A (en) * | 2000-12-26 | 2002-08-07 | 光荣工业株式会社 | Ultravoilet light/fluorescent light detection apparatus and sensing method thereof |
| CN2560943Y (en) * | 2002-08-27 | 2003-07-16 | 郭晏海 | Gel image analyser |
| CN1595115A (en) * | 2003-09-10 | 2005-03-16 | 开物科技股份有限公司 | Imaging type biological chip instrument |
| CN1595105A (en) * | 2004-07-04 | 2005-03-16 | 华中科技大学 | Integrated minisize optical analyser |
| CN1786689A (en) * | 2004-12-10 | 2006-06-14 | 国际商业机器公司 | Automated inspection system and method |
| CN101724683A (en) * | 2008-09-23 | 2010-06-09 | 米利波尔公司 | Device for microbiological analysis |
| CN201653906U (en) * | 2010-04-17 | 2010-11-24 | 上海交通大学 | Biological immunochromatography chip detector |
| CN101916029A (en) * | 2010-07-26 | 2010-12-15 | 亚亚科技股份有限公司 | High-performance illuminator for fluorescence photography |
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| Publication number | Publication date |
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| CN101893809A (en) | 2010-11-24 |
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