CN201653906U - Biological immunochromatography chip detector - Google Patents

Biological immunochromatography chip detector Download PDF

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Publication number
CN201653906U
CN201653906U CN2010201616696U CN201020161669U CN201653906U CN 201653906 U CN201653906 U CN 201653906U CN 2010201616696 U CN2010201616696 U CN 2010201616696U CN 201020161669 U CN201020161669 U CN 201020161669U CN 201653906 U CN201653906 U CN 201653906U
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China
Prior art keywords
ultraviolet
ultraviolet leds
biological
digital camera
chip detector
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Expired - Lifetime
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CN2010201616696U
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Chinese (zh)
Inventor
张雪青
崔大祥
杨浩
郭勤
王侃
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Shanghai Jiaotong University
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Shanghai Jiaotong University
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Abstract

A biological immunochromatography chip detector belonging to the technical field of bioinstrumentation comprises a digital camera, a lens, an ultraviolet LED array, a magnifying mirror and an ultraviolet filter which are arranged on the same axis in sequence from top to bottom; and the ultraviolet LED array comprises a plurality of ultraviolet LEDs which are distributed in a ring-shaped manner. The utility model has the advantages of simple and convenient operation, small volume, adoption of ring-shaped dot-matrix arrangement, realization of uniform and steady lighting and stable and reliable detection result.

Description

Biological immune chromatography chip detector
Technical field
The utility model relates to a kind of device of technical field of biological, specifically is a kind of biological immune chromatography chip detector.
Background technology
Since the nineties in 20th century, immunochromatography technique has been widely used in medical science detection, food quality monitoring, drugs detection and environmental monitoring because of its quick, easy, economic, single part of characteristic such as detection develops rapidly.At present, immunochromatography technique develops, and might become the early stage effective and sensitive sieving and diagnosis technology of pathogen, malignant tumour and angiocardiopathy etc. towards high sensitivity, direction quantitative and multivariate detection.So-called biological immune chromatography chip detector is a kind of analyser that immuno-chromatographic test paper strip detects and the result judges that carries out specially.
At present, the immuno-chromatographic test paper strip detection system comprises test strips, matched reagent and checkout equipment.After the reaction of sample to be tested and immuno-chromatographic test paper strip and matched reagent, the biological information on the test strips is by the biological information identifier, through the image acquisition of software, analyzes and adds powerful, obtains a result at last.Because immunochromatography technique is simple to operate, fast, specificity is good, and the result is clear, is easy to judge and preserve, and need not or only need simple instrument and material, so be usually used in that bedside detects and the large tracts of land examination of non-hospital.In order to cooperate these base application, the exploitation of chromatography detection system is inevitable to be developed towards miniature, portable direction.And complete detection system can help operating personnel to obtain testing result more accurately, reduces the influence and the erroneous judgement of environment.
Fluorescence quantum is the very important function nano material of a class, and its diameter is about the 1-10 nanometer.Because the yardstick quantum effect, their performances have unique photoluminescence performance.Mainly show as: emission wavelength ranges is narrow, and the Stocks displacement is big, the quantum yield height, and fluorescence lifetime is long, and chemistry and optical stability are good.Because the overlapping wide ranges of absorption spectrum, the laser that can adopt single wavelength is excitation source, realizes multi-color marking, is particularly suitable for active somatic cell imaging and polycomponent and detects simultaneously.
Find through retrieval prior art, mainly be divided into two big classes based on fluorescently-labeled biological chip testing technology at present, a class is to adopt the laser co-focusing technology, and its shortcoming is the mechanical precision condition influence that scanning accuracy is subjected to two-dimentional mobile platform, the cost of manufacture height, sweep velocity is slow; The another kind of CCD technology that is to use, the disposable whole imaging of finishing chip, sweep velocity is fast, and can not use mobile platform, shortcoming is that ratio of precision copolymerization Jiao method is low.For highdensity dot matrix chip, use the burnt technology of copolymerization usually, the low-density CCD imaging technique that then uses can satisfy the demands.Just at the demand of low end subscriber, detect principle based on CCD, cancelled the mobile platform design, the immuno-chromatographic test paper strip at single detection demand has designed low cost especially, and easily the small throughput detector that moves is easy to applying of chromatography detection technique.
Present pick-up unit adopts " biological chips detection system " of white light such as Chinese patent application number 02156427.2 record mostly, or laser carries out exciting irradiation through after the beam split to detection chip, " the matrix form biochip CCD reading and scanning apparatus " of Chinese patent application number 03149777.2 record, characteristic at the test sample burst of ultraviolel, adopt ultraviolet light directly sample to be excited, save the optical filtering light-dividing device.For the exciting of fluorescence, select for use ultraviolet lamp tube to excite mostly in addition.Generally be refraction, reach the irradiation integration of ultraviolet light, but be to use the multiple refraction of prism, just increased the volume of equipment virtually sample by prism; Also have the annular lamp tube of use that sample is carried out direct irradiation, but the heat production in the ultraviolet lamp tube use has produced influence to image quality again.
The utility model content
The utility model provides a kind of biological immune chromatography chip detector at the prior art above shortcomings, and is easy and simple to handle, volume is small and exquisite, adopts the ring shaped dot matrix formula to arrange, and realizes the uniform and stable of illumination, and testing result is reliable and stable.
The utility model is achieved through the following technical solutions, the utility model comprises: digital camera, camera lens, ultraviolet leds array, magnifying glass and ultraviolet filter, wherein: digital camera, camera lens, ultraviolet leds array, magnifying glass and ultraviolet filter from top to bottom are set in turn on the same axis, and described ultraviolet leds array is made up of several ultraviolet leds lamp ring shooting.
The utility model has adopted LED annular ultraviolet cold light source as excitation source, the CCD analog video camera cooperates image pick-up card to carry out the fluorescence signal collection, wherein led light source distributes ringwise, hole in the middle of camera lens can being passed carries out image acquisition, the LED cold light source is for directly carrying out burst of ultraviolel to sample, and do not use the mode of refraction, successfully significantly reduced the volume of biological immune chromatographic analysis instrument.
The ultraviolet LED illuminator by several independently the LED uviol lamp form, compare other illuminators, at design aspects such as range of exposures, irradiating angle and the depth of parallelisms, have bigger degree of freedom, can reduce irradiation distance, satisfy the more demands of reality.The relative conventional fluorescent lamps of LED photographic system has the longer half life period, can keep stable image input for a long time.Response time is fast.It is low to generate heat, thereby greatly reduces the interference of light source to image.
Description of drawings
Fig. 1 is the utility model structural representation.
Fig. 2 is the ultraviolet source structural drawing
Embodiment
Below embodiment of the present utility model is elaborated; present embodiment is being to implement under the prerequisite with technical solutions of the utility model; provided detailed embodiment and concrete operating process, but protection domain of the present utility model is not limited to following embodiment.
As shown in Figure 1, present embodiment comprises: digital camera 1, magnifying glass 2, ultraviolet leds array 3, camera lens 5 and ultraviolet filter 6, wherein: digital camera 1, camera lens 2, ultraviolet leds array 3, magnifying glass 5 and ultraviolet filter 6 from top to bottom are set in turn on the same axis, and several ultraviolet leds 4 annular spread are in the inside of ultraviolet leds array 3.
Digital camera 1 front end is connected to magnifying glass 2, camera lens 5, ultraviolet filter 6 successively, and 1 pair of image of digital camera is gathered, and links to each other with the computing machine that the special image capture card is housed, and carries out the transmission of data.Ultraviolet filter 6 can the filtering ultraviolet light interference.Ultraviolet leds array 3 is an annular light source, and the centre is empty, the front end ultraviolet leds 4 that evenly distributes, and irradiated plane 7 is the ultraviolet irradiation scope.The camera lens part (magnifying glass 2, camera lens 5, ultraviolet filter 6 integral body) of digital camera 1 is passed the middle part of ultraviolet leds array 3.Immuno-chromatographic test paper strip 8 places in the ultraviolet irradiation scope 7, carry out fluorescence excitation by the test strip 9 on 3 pairs of immuno-chromatographic test paper strips 8 of ultraviolet leds array, emission light carries out the signal amplification by camera lens 5 and magnifying glass 2 and is delivered to digital camera 1 after ultraviolet filter 6 filters, the machine image pick-up card is delivered to computing machine with detected image as calculated, carries out the analyzing and processing of testing result.
Ultraviolet leds array 3 can provide uniform radiation response in range of exposures 7, make that the test strip 9 on the immuno-chromatographic test paper strip 8 obtains good exciting.Compare other illuminators,, have bigger degree of freedom, can reduce irradiation distance, satisfy the more demands of reality at design aspects such as range of exposures, irradiating angle and the depth of parallelisms.The relative conventional fluorescent lamps of LED photographic system has the longer half life period, can keep stable image input for a long time.Response time is fast.It is low to generate heat, thereby greatly reduces the interference of light source to image.

Claims (4)

1. biological immune chromatography chip detector, it is characterized in that, comprise: digital camera, camera lens, ultraviolet leds array, magnifying glass and ultraviolet filter, wherein: digital camera, camera lens, ultraviolet leds array, magnifying glass and ultraviolet filter from top to bottom are set in turn on the same axis.
2. biological immune chromatography chip detector according to claim 1 is characterized in that, described ultraviolet leds array is made up of several ultraviolet leds lamp ring shooting.
3. according to claim 1 or 2 described biological immune chromatography chip detectors, it is characterized in that described ultraviolet leds array is an annular light source, the centre is empty, the front end ultraviolet leds that evenly distributes.
4. biological immune chromatography chip detector according to claim 1 is characterized in that described digital camera front end links to each other with the computing machine that the special image capture card is housed, and carries out the transmission of data.
CN2010201616696U 2010-04-17 2010-04-17 Biological immunochromatography chip detector Expired - Lifetime CN201653906U (en)

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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101893809A (en) * 2010-06-30 2010-11-24 亚亚科技股份有限公司 Light source device for fluorescence photography
CN103308676A (en) * 2013-05-31 2013-09-18 中国科学院上海光学精密机械研究所 Gold strip reader and detection method
CN103808918A (en) * 2014-01-27 2014-05-21 上海耀研光电科技有限公司 Image-sensor-based detection system of colloidal gold immunoassay
CN103954559A (en) * 2014-04-02 2014-07-30 中国农业大学 Multi-wavelength large power LED spot light source apparatus
CN104914239A (en) * 2015-06-29 2015-09-16 上海交通大学 Immunochromatographic test strip detection equipment
CN104949997A (en) * 2014-03-25 2015-09-30 克朗斯股份公司 Inspection device and inspection method for inspecting containers by means of illumination
CN105259151A (en) * 2015-11-02 2016-01-20 深圳市锦瑞电子有限公司 Fluorescence detection system and instrument
CN106124778A (en) * 2016-07-27 2016-11-16 深圳市博卡生物技术有限公司 Immunoblotting assay instrument
CN106290362A (en) * 2016-07-29 2017-01-04 江苏量点科技有限公司 Equipment and using method thereof for quantum dot test strips quality inspection
CN107613162A (en) * 2017-08-11 2018-01-19 浙江大学 A kind of portable micro-fluidic chip imaging system
CN108627482A (en) * 2017-03-20 2018-10-09 上海溯源生物技术有限公司 Fluorescence detection device and its fluorimetric analysis method based on mobile terminal
CN108801995A (en) * 2018-06-05 2018-11-13 浙江大学 A kind of the high pass amount detecting device and method of incorporating quantum point fluorescence and multispectral camera
EP3712613A4 (en) * 2017-11-22 2021-06-30 National University Corporation Tokyo Medical and Dental University Biogas measurement device

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101893809B (en) * 2010-06-30 2012-07-25 亚亚科技股份有限公司 Light source device for fluorescence photography
CN101893809A (en) * 2010-06-30 2010-11-24 亚亚科技股份有限公司 Light source device for fluorescence photography
CN103308676A (en) * 2013-05-31 2013-09-18 中国科学院上海光学精密机械研究所 Gold strip reader and detection method
CN103808918A (en) * 2014-01-27 2014-05-21 上海耀研光电科技有限公司 Image-sensor-based detection system of colloidal gold immunoassay
CN104949997A (en) * 2014-03-25 2015-09-30 克朗斯股份公司 Inspection device and inspection method for inspecting containers by means of illumination
CN103954559B (en) * 2014-04-02 2016-07-20 中国农业大学 Multi-wavelength great power LED spotlight source device
CN103954559A (en) * 2014-04-02 2014-07-30 中国农业大学 Multi-wavelength large power LED spot light source apparatus
CN104914239A (en) * 2015-06-29 2015-09-16 上海交通大学 Immunochromatographic test strip detection equipment
CN105259151A (en) * 2015-11-02 2016-01-20 深圳市锦瑞电子有限公司 Fluorescence detection system and instrument
CN106124778A (en) * 2016-07-27 2016-11-16 深圳市博卡生物技术有限公司 Immunoblotting assay instrument
CN106290362A (en) * 2016-07-29 2017-01-04 江苏量点科技有限公司 Equipment and using method thereof for quantum dot test strips quality inspection
CN108627482A (en) * 2017-03-20 2018-10-09 上海溯源生物技术有限公司 Fluorescence detection device and its fluorimetric analysis method based on mobile terminal
CN107613162A (en) * 2017-08-11 2018-01-19 浙江大学 A kind of portable micro-fluidic chip imaging system
CN107613162B (en) * 2017-08-11 2020-11-20 浙江大学 Portable micro-fluidic chip imaging system
EP3712613A4 (en) * 2017-11-22 2021-06-30 National University Corporation Tokyo Medical and Dental University Biogas measurement device
CN108801995A (en) * 2018-06-05 2018-11-13 浙江大学 A kind of the high pass amount detecting device and method of incorporating quantum point fluorescence and multispectral camera

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Granted publication date: 20101124