CN108181290A - A kind of quantitative fluorescence analysis instrument - Google Patents
A kind of quantitative fluorescence analysis instrument Download PDFInfo
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- CN108181290A CN108181290A CN201810248662.9A CN201810248662A CN108181290A CN 108181290 A CN108181290 A CN 108181290A CN 201810248662 A CN201810248662 A CN 201810248662A CN 108181290 A CN108181290 A CN 108181290A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention provides a kind of quantitative fluorescence analysis instrument, it is related to medical detecting Instrument technical field, the analyzer includes the fish tail handle of fuselage, the thermal printer that fuselage roof is installed and fuselage bottom installation, fuselage is hollow rectangular parallelepiped structure, the front of fuselage is provided with the liquid crystal display for display data, and the side of fuselage is provided with the detection entrance for being placed in examined object；The front of fish tail handle is provided with the keyboard for information exchange, and the back side of fuselage is provided with the battery case for placing battery.Quantitative fluorescence analysis instrument provided by the invention is compact-sized, portable easy-to-use, blood of human body and urine are analyzed and detected with immunochromatography technique principle based on fluorescence immunoassay, solves the technical issues of existing analyzer mechanical transmission structure is complicated, light path devices utilization rate is low.
Description
Technical field
The present invention relates to medical detecting Instrument technical field more particularly to a kind of quantitative fluorescence analysis instrument.
Background technology
Fluoroimmunoassay（FIA）It is a kind of micro-analysis method developed in recent years, is most sensitive at present
One of trace analysis.The molecule of Cucumber can absorb energy and launch fluorescence, strong according to the spectrum and fluorescence of fluorescence
Degree carries out substance qualitative or quantitative method, referred to as fluorescence analysis.Fluoroimmunoassay（FIA）With it is highly sensitive,
Selectivity is strong, needs the advantages that sample size is few and method is easy, and the Determination Limit of this method is usually 2~4 higher than spectrophotometry
The order of magnitude, the application in biochemical analysis are wide.
The general usual volume of fluorescent quantitative detector is larger at present, is not easy to portable use, therefore, limit its
It has inconvenient traffic, the development of under-developed area.Even if there is the product of some commercializations, there is also complicated, poor reliability, essences
The shortcomings of degree is not high, and operation difficulty is big.Therefore, realize that instrument is portable and scalability is this field problem to be solved.
Invention content
In view of the above problems, the present invention is to solve to provide a kind of quantitative fluorescence analysis instrument compact-sized, portable easy-to-use,
Blood of human body and urine are analyzed and detected with immunochromatography technique principle based on fluorescence immunoassay, solves existing analyzer
The technical issues of mechanical transmission structure is complicated, light path devices utilization rate is low.
The present invention provides a kind of quantitative fluorescence analysis instrument, which is characterized in that the temperature-sensitive installed including fuselage, fuselage roof is beaten
Print machine and the fish tail handle of fuselage bottom installation, wherein：The fuselage is hollow rectangular parallelepiped structure, and the front setting of fuselage is useful
In the liquid crystal display of display data, the side of fuselage is provided with the detection entrance for being placed in object to be checked；The fish tail handle is just
Face is provided with the keyboard for information exchange, and the back side of fuselage is provided with the battery case for placing battery；
Further include the detection card for being inserted into detection entrance, the test paper that the detection card includes detection plate, is placed in detection plate
The sample-adding pad set between item and detection plate and test strips, wherein：The recess that faces down of the detection plate, which is formed, holds examination
The concave station of paper slip, the back side of detection plate are provided with driving rack, and one end of detection plate extends inwardly to form hanging platform after holding up, and examines
The other end of drafting board is holded up to form the support plate highly consistent with hanging platform, and the hanging platform is located at the top of sample-adding pad, vacantly
Well is offered on platform；
The fuselage interior is provided with the sense channel of communication with detection entrance, and observation window is provided at the top of the sense channel,
The bottom of sense channel is provided with transmission notch；
The fuselage interior is additionally provided with the scanning transmission module for driving detection card mobile, and the scanning transmission module includes transmission
The microswitch of gear, the stepper motor with nutating gear rotation and sensing detection card position, the transmission gear setting
It is moved repeatedly by engaged transmission rack drives detection card in the transmission notch, the microswitch is arranged on sense channel
Bottom；
The fuselage interior is additionally provided with the first light source channel for generating light source, and the first light source channel is parallel to detection
Channel and the light source optical filter including the light source being coaxially disposed and with light source side；
The fuselage interior be additionally provided with for receive feedback fluorescence the first reflection channel, first reflection channel perpendicular to
Sense channel and the first planoconvex spotlight including being coaxially disposed, detection optical filter, the second planoconvex spotlight, grating and the first fluorescence
Sensor is provided in the axis intersection of the first reflection channel and first light source channel and tilts 45 ° of dichroic mirrors for light splitting,
Wherein：First planoconvex spotlight be mounted on the observation window top, the dichroic mirror be located at first planoconvex spotlight and
It is located at the homonymy of light source between detection optical filter and with light source optical filter, first fluorescent optical sensor receives instead for measuring
Present the numerical value of fluorescence.
Further, the fuselage interior is additionally provided with collection fibers, the second fluorescent optical sensor and two groups of coupling assemblies, institute
It states fuselage side and corresponds to the positions of collection fibers and be additionally provided with optical fiber interface, wherein：The collection fibers include importing light
The incident optical in source, for the reception optical fiber that exports fluorescence and for collecting summarizing for the incident optical and reception optical fiber
End；Coupling assembly is provided between the incident optical and light source, is also set between the reception optical fiber and the second fluorescent optical sensor
It is equipped with coupling assembly；The end of summarizing is mounted in optical fiber interface.
Further, the collection fibers include parallel collection optical fiber or vertical collection fibers, wherein：
The parallel collection optical fiber includes the collimated optical fiber being mutually parallel, parallel reception optical fiber summarizes end with parallel, described
Collimated optical fiber is for importing light source, and for the parallel reception optical fiber for exporting fluorescence, the parallel end of summarizing is mounted on light
In fine interface and for collecting the collimated optical fiber and parallel reception optical fiber；
The vertical collection fibers include the vertical incidence optical fiber that is mutually parallel and vertical reception optical fiber and with it is described it is vertical enter
Penetrate optical fiber, vertical reception optical fiber is respectively perpendicular it is vertical summarize end, the vertical incidence optical fiber for importing light source, it is described vertically
For exporting fluorescence, the vertical end of summarizing is mounted in optical fiber interface and for collecting the vertical incidence optical fiber reception optical fiber
With vertical reception optical fiber.
Further, the parallel end of summarizing is by being located at the collimated optical fiber at center and evenly around described parallel
The parallel reception optical fiber composition of incident optical.
Further, the vertical end of summarizing is by being located at the vertical incidence optical fiber at center and evenly around described vertical
Vertical reception optical fiber composition on the outside of incident optical.
Further, the fiber optic bundle being detachably connected with optical fiber interface is further included, described fiber optic bundle one end passes through connector
Connection collection fibers summarize end, and the fiber optic bundle other end connects optical fiber probe by connector.
Further, the coupling assembly includes the first convex lens, spike filter and the second convex lens of coaxial arrangement.
Further, it is provided with to capture the detection band of antigen antibody complex, free for being enriched on the test paper
The control band of fluorescent marker and the Quick Response Code for record number.
Further, circuit board is additionally provided in the fuselage, the circuit board includes main control chip, amplification filtered electrical
Road, fluorescent optical sensor, light source driving circuit and the ID chips being respectively connected with the control chip, power circuit, A/D conversions
Circuit, time sequence driving circuit, synchronization control circuit and motor-drive circuit, wherein：
The main control chip connects liquid crystal display, keyboard and thermal printer respectively；
The main control chip passes sequentially through A/D conversion circuits, filtering and amplifying circuit obtains fluorescent optical sensor information；
The fluorescent optical sensor connects the time sequence driving circuit；
The main control chip passes sequentially through synchronization control circuit, light source driving circuit driving light source working；
The main control chip is controlled by clicking driving circuit Driving Stepping Motor；
The main control chip connects the positive and negative anodes in battery case by power circuit.
Further, the light source includes laser light source, hernia lamp source.
A kind of quantitative fluorescence analysis instrument of the present invention has the advantages that：
1）This quantitative fluorescence analysis instrument is compact-sized, using detection card backside rack and the transmission of stepping motor rotating shaft gear cooperation
The motion platform scheme of transmission is substituted in scheme, reduces the devices such as sliding block, ruler belt, guide rail, test strips frid, positioning device
Part.Meanwhile the shape by designing detection plate, it on the one hand can utilize the guiding detection card movement of sense channel shape, the opposing party
Face can facilitate user to drip sample.
2）This quantitative fluorescence analysis instrument is portable easy-to-use, and analyzer includes thermal printer, battery case and liquid crystal display, can
It is continuing in the case of no extraneous power supply.
3）This quantitative fluorescence analysis instrument scalability is strong, has external optical fiber interface, passes through fiber optic bundle, connector and optical fiber
Probe can be directly outside analyzer reaction zone.Meanwhile by rationally designing light path, common light source.
Description of the drawings
It in order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be in embodiment or description of the prior art
Required attached drawing is briefly described, it should be apparent that, the accompanying drawings in the following description is only some realities of the present invention
Example is applied, it for those of ordinary skill in the art, without creative efforts, can also be according to these attached drawings
Obtain other attached drawings.
Fig. 1 is first embodiment of the present invention positive structure schematic；
Fig. 2 is first embodiment of the present invention structure schematic diagram；
Fig. 3 is first embodiment of the present invention side structure schematic diagram；
Fig. 4 is the detection card internal structure schematic diagram of the first embodiment of the present invention；
Fig. 5 is the detection card positive structure schematic of the first embodiment of the present invention；
Fig. 6 is the detection card backside structure diagram of the first embodiment of the present invention；
Fig. 7 is the fuselage interior structure diagram of the first embodiment of the present invention；
Fig. 8 is the detection card operation principle schematic diagram of the first embodiment of the present invention；
Fig. 9 is the circuit connection diagram of the first embodiment of the present invention；
Figure 10 is the light source driving circuit connection diagram of the first embodiment of the present invention；
Figure 11 is the filtering and amplifying circuit connection diagram of the first embodiment of the present invention；
Figure 12 is the A/D conversion circuit connection diagrams of the first embodiment of the present invention；
Figure 13 is the side structure schematic diagram of the second embodiment of the present invention；
Figure 14 is the positive structure schematic of the second embodiment of the present invention；
Figure 15 is the fuselage interior structure diagram of the second embodiment of the present invention；
Figure 16 is the coupling assembly structure diagram of the second embodiment of the present invention；
Figure 17 is the parallel collection optical fiber structure schematic diagram of the second embodiment of the present invention；
Figure 18 is the fiber bundle structure schematic diagram of the second embodiment of the present invention；
Figure 19 is the fibre-optic connector structure schematic diagram of the third embodiment of the present invention.
Figure 20 is the fuselage interior structure diagram of the third embodiment of the present invention；
Figure 21 is the vertical collection fibers structure diagram of the third embodiment of the present invention；
Figure 22 is the fiber bundle structure schematic diagram of the third embodiment of the present invention；
In figure：1- fuselages, 101- liquid crystal displays, 102- detections entrance, 103- optical fiber interfaces, 2- fish tails handle, 201- keyboards, 202-
Battery case, 3- thermal printers, 4- detections card, 401- detection plates, 402- test strips, 403- sample-adding pad, 4011- concave stations, 4012-
Hanging platform, 4013- support plates, 4014- wells, 4015- racks, 4021- detections band, 4022- controls band, 4023- bar codes,
5- sense channels, 501- observation windows, 502- transmission notch, 6- scanning transmission module, 601- transmission gears, 602- stepper motors,
603- microswitch 7- first light sources channel, 701- light sources, 702- light sources optical filter, the first reflection channels of 8-, 801- first are flat
Convex lens, 802- dichroic mirrors, 803- detections optical filter, the second planoconvex spotlights of 804-, 805- gratings, the first fluorescence senses of 806-
Device, 9- circuit boards, 10- coupling assemblies, the first convex lenses of 1001-, 1002- spike filters, the second convex lenses of 1003-, 11-
Two fluorescent optical sensors, 12- parallel collections optical fiber, 1201- collimated optical fibers, the parallel reception optical fibers of 1202-, the parallel remittances of 1203-
Total end, the vertical collection fibers of 13-, 1301- vertical incidence optical fiber, 1302- vertical receptions optical fiber, 1303- vertically summarize end, 14-
Fiber optic bundle, 15- connectors, 16- optical fiber probes.
Specific embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other without creative efforts
Embodiment shall fall within the protection scope of the present invention.
Embodiment one
As shown in Figure 1, a kind of quantitative fluorescence analysis instrument, the thermal printer 3 and fuselage 1 installed including fuselage 1,1 top of fuselage
The fish tail handle 2 of bottom installation, wherein：The fuselage 1 is hollow rectangular parallelepiped structure, and the front of fuselage 1 is provided with to show
The liquid crystal display 101 of data, the side of fuselage 1 are provided with the detection entrance 102 for being placed in object to be checked；The fish tail handle 2
Front is provided with the keyboard 201 for information exchange, and the back side of fuselage 1 is provided with the battery case 202 for placing battery, battery
The position of box 202 is as shown in Figure 2.Specifically, thermal printer 3 can print detection data.
As shown in Figure 3,4, the detection card 4 for being inserted into detection entrance 102 is further included, the detection card 4 includes detection plate
401st, the sample-adding pad 403 set between the test strips 402 and detection plate 401 and test strips 402 that are placed in detection plate 401,
Wherein：The recess that faces down of the detection plate 401 forms the concave station 4011 for holding test strips 402, as shown in Figure 5；Detection plate
401 back side is provided with driving rack 4015, as shown in Figure 6；One end of detection plate 401 extends inwardly to form hanging platform after holding up
4012, the other end of detection plate 401 is holded up to form the support plate 4013 highly consistent with hanging platform 4012, the hanging platform 4012
Positioned at the top of sample-adding pad 403, well 4014 is offered on hanging platform 4012.
Specifically, the height of hanging platform 4012 and support plate 4013 should be consistent, and should be slightly below in sense channel
Highly, it can smoothly be moved and not shaken in sense channel with guarantee detection card 4.
Specifically, it is provided in the test strips 402 for capturing the detection band 4021 of antigen antibody complex, for richness
The control band 4022 and the Quick Response Code 4023 for record number of the free fluorescent marker of collection.
Test process example：Detection buffer solution (the CRP antibody 1 for being fluorescently labeled substance markers) and blood sample are mixed first
It closes, detects the reactions of the CRP (antigen) in CRP antibody 1 and blood in buffer solution and form antigen antibody complex.Work as mixture
After sample immerses test strips 402 by well 4014, test strips 402, capillarity can be run through and swum in test strips 402
Dynamic, the CRP antibody 2 that CRP antigen antibody complexs are fixed on detection band 4021 is captured, and is enriched with or is trapped in test strips
On 402 detection band 4021, and free fluorescent marker can then cross detection band 4021 and be enriched at control band 4022.Blood
CRP in sample is more, detect band 4021 on compound gather more, detect band 4021 on fluorescent material content with
CRP concentration has certain correspondence.
As shown in fig. 7, the fuselage 1 is internally provided with the sense channel 5 of communication with detection entrance 102, the sense channel 5
Top be provided with observation window 501, the bottom of sense channel 5 is provided with transmission notch 502；It is additionally provided with inside the fuselage 1
The scanning transmission module 6 of 4 movement of detection card is driven, the scanning transmission module 6 includes transmission gear 601, band nutating gear
603 passes are opened in the fine motion of 4 positions of stepper motor 602 and sensing detection card of 601 rotations, and the transmission gear 601 is arranged on institute
State in transmission notch 502 drives detection card 4 to move repeatedly by engaged transmission rack 4015, and the fine motion is opened 603 passes and is arranged on
The bottom of sense channel 5.
Specifically, the outer profile of detection card 4 is slightly less than the outer profile of sense channel 5.When user is by detection card 4 to be detected
After being put into sense channel 5, the bottom rack 4015 of detection card 4 is engaged with the transmission gear 601 of 5 bottom of sense channel, works as detection
After card 4 is put into sense channel 5 completely, the fine motion for depressing 5 bottom of sense channel is opened 603 passes by detection card 4, and fine motion at this time opens 603
It closes and triggering information is fed back into main control chip, after main control chip obtains the information that detection card 4 is fully inserted into, start to control stepping electricity
602 forward and backward of machine is repeatedly scanned with realizing that detection blocks 4.
Specifically, stepper motor 602 selects 6.5MM two-phase four-wire types stepper motor 602.Preferably, it is detected to reduce
Card 4 and the frictional resistance of 5 bottom of sense channel can set ball or roller bearing so that slide in the inner wall bottom of sense channel 5
Friction becomes rolling friction, the material of low-friction coefficient can also be used to make detection card 4 and sense channel 5, such as nylon material.
As shown in fig. 7, the first light source channel 7 for generating light source is additionally provided with inside the fuselage 1, first light
Source channels 7 are parallel to sense channel 5 and the light source optical filter including the light source 701 being coaxially disposed and with 701 side of light source
702；The first reflection channel 8 for receiving feedback fluorescence is additionally provided with inside the fuselage 1, first reflection channel 8 hangs down
Directly in sense channel 5 and including be coaxially disposed the first planoconvex spotlight 801, detection optical filter 803, the second planoconvex spotlight 804,
805 and first fluorescent optical sensor 806 of grating is set in the axis intersection of the first reflection channel 8 and 701 channel 7 of first light source
Have and tilt 45 ° of dichroic mirrors 802 for light splitting, wherein：First planoconvex spotlight 801 is mounted on the top of the observation window 501
Portion, the dichroic mirror be located between first planoconvex spotlight 801 and detection optical filter 803 and with light source optical filter 702
In the homonymy of light source 701, first fluorescent optical sensor 806 receives the numerical value of feedback fluorescence for measuring.
Specifically, above-mentioned grating 805 is aperture grating 805.
As shown in figure 8, the course of work of 701 channel 7 of first light source and the first reflection channel 8 is as follows：Light source 701 during detection
It lights, by the light splitting dichroic mirror of 45 ° of placements after the collimated optical filter of exciting light, then by the first planoconvex spotlight of focusing/collimation 801
Focus on 402 surface of test strips.At this point, stepper motor 602 drives test strips 402 steadily mobile.
Excite the detection zone in optical scanning test strips 402.Fluorescent material in test strips 402 in detection zone is excited
The fluorescence gone out is collimated by the first planoconvex spotlight 801 as directional light, and veiling glare is filtered out through dichroic mirror and detection optical filter 803, then by the
Two planoconvex spotlights 804 are focused on the burnt aperture of copolymerization.It is detected and is converted by the first fluorescent optical sensor 806 by the light of diaphragm
For electric signal, which can be converted into the concentration of tested sample after handling and analyzing and calculate.
As shown in figure 9, being additionally provided with circuit board 9 in the fuselage 1, the circuit board 9 includes main control chip, amplification filtering
Circuit, fluorescent optical sensor, 701 driving circuit of light source and ID chips, power circuit, the A/ being respectively connected with the control chip
D conversion circuits, time sequence driving circuit, synchronization control circuit and motor-drive circuit, wherein：The main control chip connects liquid respectively
Crystalline substance screen 101, keyboard 201 and thermal printer 3；The main control chip passes sequentially through A/D conversion circuits, filtering and amplifying circuit obtains
Fluorescent optical sensor information；The fluorescent optical sensor connects the time sequence driving circuit；The main control chip passes sequentially through synchronous control
Circuit processed, 701 driving circuit of light source driving light source 701 work；The main control chip drives stepping electricity by clicking driving circuit
Machine 602 controls；The main control chip connects the positive and negative anodes in battery case 202 by power circuit.
In the present embodiment, light source 701 can be hernia lamp source 701.
Figure 10 is the 701 driving circuit connection diagram of light source of the first embodiment of the present invention, by power supply to energy storage
Capacitance C charges, and voltage stabilizing control element is started to work when the voltage at its both ends reaches preset value, makes its invariable.When
When needing flash of light, trigger circuit is controlled by interface circuit by main control chip, capacitance E generates forceful electric power through resistance Rl repid discharges
Stream, triggered pulse transformer generate the high electric field pulse of volts up to ten thousand in its secondary grade, make the gas ionization in xenon lamp, greatly reduce
The internal resistance of lamp, a large amount of electric energy stored in storage capacitor C are released within the extremely short time by xenon flash lamp, so as to generating pole
For strong flash of light.
The present embodiment is reversely amplified using CA3450 operational amplifiers, although CA3450 is completed to the anti-of signal
To amplification, but noise is bigger in its output signal, and filter circuit is needed to filter out noise, improves signal quality.Since it is desired that
The signal frequency arrived is than relatively low, so common voltage controlled voltage source second-order low-pass filter is selected to filter out noise, as shown in figure 11.
The present embodiment samples and is kept for the stage mainly realized by sampling/retainer, and quantifies main with coding stage
It is realized by A/D converter, the A/D converter of the present embodiment selects MAX120.Gain tuning and dual polarity bias tune
It is whole, it is realized by the potentiometer RP2 and RP1 in Figure 12, biasing adjustment should be prior to Gain tuning in adjustment.Between the two adjustment
There may be some to influence each other, need to adjust repeatedly.The adjustment of biasing and gain is the subdivision converted to A/D, it is therefore intended that is carried
The precision of high A/D.
TCD1200D types CCD or silicon photocell may be used in the fluorescent optical sensor of the present embodiment.
Quantitative fluorescence analysis instrument provided in this embodiment is compact-sized, portable easy-to-use, based on fluorescence immunoassay and immunochromatography
Technical principle is analyzed and is detected to blood of human body and urine, solves existing analyzer mechanical transmission structure complexity, light path
The technical issues of devices use rate is low.
Embodiment two
As shown in Figure 13, Figure 14, the present embodiment extends detection fiber that can be external, Ke Yizhi on the basis of embodiment one
It connects for the measurement in analyzer external reaction pond, is detected suitable for Pesticides Testing, seaweed.Collect specifically, 1 side of fuselage corresponds to
The position of optical fiber is additionally provided with optical fiber interface 103, and the present embodiment further includes the fiber optic bundle being detachably connected with optical fiber interface 103
14, end is summarized in described 14 one end of fiber optic bundle by the connection collection fibers of connector 15, and 14 other end of fiber optic bundle passes through company
It connects device 15 and connects optical fiber probe 16.It is visited specifically, connector 15 can connect multimode plastic optical fiber beam, single mode optical fiber and optical fiber
Needle, so as to generate the transmission road of fluorescence signal and exciting light.Specifically, fiber optic bundle 14 can be according to circumstances small-sized using armouring
Flexible optical cable.
As shown in figure 15, parallel collection optical fiber 12, the second fluorescent optical sensor 11 and two groups of coupling assemblies are specifically further included
10, wherein：The parallel collection optical fiber 12 is included for importing the collimated optical fiber 1201 of light source 701, for exporting fluorescence
Parallel reception optical fiber 1202 and for collecting parallel remittance of the collimated optical fiber 1201 with parallel reception optical fiber 1202
Total end 1203；Coupling assembly 10, the parallel reception optical fiber are provided between the collimated optical fiber 1201 and light source 701
1202 and second are also equipped with coupling assembly 10 between fluorescent optical sensor 11；The parallel end 1203 of summarizing is mounted on optical fiber interface
In 103.
Wherein coupling assembly 10 is as shown in figure 16, and coupling assembly 10 includes the first convex lens 1001 of coaxial arrangement, narrowband
1002 and second convex lens 1003 of optical filter, using coupled lens, on the one hand to exciting light beam collimation, to improve the note of probe
Enter efficiency；On the other hand it can collect fluorescence signal, to improve the utilization rate of fluorescence signal.
Wherein parallel collection optical fiber 12 is as shown in figure 17, parallel to summarize end 1203 as shown in figure 18, due to parallel input light
The emergent light spot of fibre 1201 is that circle is symmetrical, and obtained fluorescence hot spot is also round symmetrically.Therefore, using the optical fiber of symmetric distributed
Beam 14 receives reflective light intensity to improve the efficiency and the sensitivity that receive light, in order to reduce the loss in transmission process, the light used
Shorter, the about 60-80cm of fibre.
As shown in figure 19, light source 701 and the simplest method of fiber coupling are direct-couplings, i.e., optical fiber planar end surface is direct
The light-emitting surface of alignment light source 701.This method is simple and convenient, but coupling efficiency is low.If the numerical aperture of optical fiber is 0.26, directly
The peak efficiency for connecing coupling is only 6.7%.By placing convergent lens between light source 701 and fiber end face, change xenon flash lamp
Luminous direction or the acceptance angle of optical fiber can improve coupling efficiency.Consider detection environment, the present embodiment final choice is complete
Optical-fiber type structure.
The present embodiment has external optical fiber interface 103, can be direct by fiber optic bundle 14, connector 15 and optical fiber probe 16
Reaction zone outside analyzer is particularly suitable for the quick detection under ocean condition.Meanwhile by rationally designing light path, share light
Source 701.
Embodiment three
Compared with embodiment two, the collection fibers structure of embodiment three is different, and as shown in figure 20, embodiment three includes vertically collecting
Optical fiber 13, the second fluorescent optical sensor 11 and two groups of coupling assemblies 10, the position that 1 side of fuselage corresponds to collection fibers are also set up
There is optical fiber interface 103, wherein as shown in figure 21, vertical collection fibers 13 include the vertical incidence optical fiber 1301 being mutually parallel and hang down
Straight reception optical fiber 1302 and vertical summarize with what the vertical incidence optical fiber 1301, vertical reception optical fiber 1302 were respectively perpendicular
End, for the vertical incidence optical fiber 1301 for importing light source 701, the vertical reception optical fiber 1302 is described for exporting fluorescence
Vertical end 1303 of summarizing is mounted in optical fiber interface 103 and for collecting the vertical incidence optical fiber 1301 and vertical reception optical fiber
1302.As shown in figure 22, vertically summarize end 1303 by being located at the vertical incidence optical fiber 1301 at center and evenly around described
The vertical reception optical fiber 1302 in 1301 outside of vertical incidence optical fiber forms.
Specifically, since the emergent light spot of collimated optical fiber 1201 is that circle is symmetrical, obtained fluorescence hot spot is also round
Symmetrically.Therefore, the fiber optic bundle 14 of symmetric distributed is used to receive reflective light intensity to improve the efficiency and the sensitivity that receive light, in order to
The loss in transmission process is reduced, the optical fiber used is shorter, about 60-80cm.
C reactive protein is detected using the present embodiment, in the range of the reagent concentration of 3.00~39.00mg/L, every
The same fluorescent reagent that 3mg/L chooses concentration level carries out test analysis, each concentration level test be averaged for 3 times for
Final result.Linear regression analysis is carried out to the result measured, the equation of linear regression of gained is:Y=0.872x+0.3, wherein
Coefficient R2=0.998, the above result shows that instrument has good linear sound in 3.00~39.00mg/L concentration ranges
Answer characteristic.
The present embodiment has external optical fiber interface 103, can be direct by fiber optic bundle 14, connector 15 and optical fiber probe 16
Reaction zone outside analyzer is particularly suitable for the quick detection under ocean condition.Meanwhile by rationally designing light path, share light
Source 701.
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
The present invention is described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that：It still may be used
To modify to the technical solution recorded in foregoing embodiments or carry out equivalent replacement to which part technical characteristic；
And these modification or replace, various embodiments of the present invention technical solution that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (10)
1. a kind of quantitative fluorescence analysis instrument, which is characterized in that the thermal printer installed including fuselage, fuselage roof and fuselage bottom
The fish tail handle of portion's installation, wherein：The fuselage is hollow rectangular parallelepiped structure, and the front of fuselage is provided with for display data
Liquid crystal display, the side of fuselage are provided with the detection entrance for being placed in object to be checked；The front of the fish tail handle, which is provided with, to be used for
The keyboard of information exchange, the back side of fuselage are provided with the battery case for placing battery；
Further include the detection card for being inserted into detection entrance, the test paper that the detection card includes detection plate, is placed in detection plate
The sample-adding pad set between item and detection plate and test strips, wherein：The recess that faces down of the detection plate, which is formed, holds examination
The concave station of paper slip, the back side of detection plate are provided with driving rack, and one end of detection plate extends inwardly to form hanging platform after holding up, and examines
The other end of drafting board is holded up to form the support plate highly consistent with hanging platform, and the hanging platform is located at the top of sample-adding pad, vacantly
Well is offered on platform；
The fuselage interior is provided with the sense channel of communication with detection entrance, and observation window is provided at the top of the sense channel,
The bottom of sense channel is provided with transmission notch；
The fuselage interior is additionally provided with the scanning transmission module for driving detection card mobile, and the scanning transmission module includes transmission
The microswitch of gear, the stepper motor with nutating gear rotation and sensing detection card position, the transmission gear setting
It is moved repeatedly by engaged transmission rack drives detection card in the transmission notch, the microswitch is arranged on sense channel
Bottom；
The fuselage interior is additionally provided with the first light source channel for generating light source, and the first light source channel is parallel to detection
Channel and the light source optical filter including the light source being coaxially disposed and with light source side；
The fuselage interior be additionally provided with for receive feedback fluorescence the first reflection channel, first reflection channel perpendicular to
Sense channel and the first planoconvex spotlight including being coaxially disposed, detection optical filter, the second planoconvex spotlight, grating and the first fluorescence
Sensor is provided in the axis intersection of the first reflection channel and first light source channel and tilts 45 ° of dichroic mirrors for light splitting,
Wherein：First planoconvex spotlight be mounted on the observation window top, the dichroic mirror be located at first planoconvex spotlight and
It is located at the homonymy of light source between detection optical filter and with light source optical filter, first fluorescent optical sensor receives instead for measuring
Present the numerical value of fluorescence.
2. quantitative fluorescence analysis instrument according to claim 1, which is characterized in that the fuselage interior, which is additionally provided with, collects light
Fine, the second fluorescent optical sensor and two groups of coupling assemblies, the position that the fuselage side corresponds to collection fibers are additionally provided with optical fiber and connect
Mouthful, wherein：The collection fibers are included for importing the incident optical of light source, for exporting the reception optical fiber of fluorescence, Yi Jiyong
Summarize end in collect the incident optical and reception optical fiber；Coupling assembly, institute are provided between the incident optical and light source
It states and is also equipped with coupling assembly between reception optical fiber and the second fluorescent optical sensor；The end of summarizing is mounted in optical fiber interface.
3. quantitative fluorescence analysis instrument according to claim 2, which is characterized in that the collection fibers include parallel collection light
Fine or vertical collection fibers, wherein：
The parallel collection optical fiber includes the collimated optical fiber being mutually parallel, parallel reception optical fiber summarizes end with parallel, described
Collimated optical fiber is for importing light source, and for the parallel reception optical fiber for exporting fluorescence, the parallel end of summarizing is mounted on light
In fine interface and for collecting the collimated optical fiber and parallel reception optical fiber；
The vertical collection fibers include the vertical incidence optical fiber that is mutually parallel and vertical reception optical fiber and with it is described it is vertical enter
Penetrate optical fiber, vertical reception optical fiber is respectively perpendicular it is vertical summarize end, the vertical incidence optical fiber for importing light source, it is described vertically
For exporting fluorescence, the vertical end of summarizing is mounted in optical fiber interface and for collecting the vertical incidence optical fiber reception optical fiber
With vertical reception optical fiber.
4. quantitative fluorescence analysis instrument according to claim 3, which is characterized in that the parallel end of summarizing is by being located at center
Collimated optical fiber and evenly around the collimated optical fiber parallel reception optical fiber composition.
5. quantitative fluorescence analysis instrument according to claim 3, which is characterized in that the vertical end of summarizing is by being located at center
Vertical incidence optical fiber and evenly around the vertical incidence outer fiber vertical reception optical fiber composition.
6. quantitative fluorescence analysis instrument according to claim 4 or 5, which is characterized in that further include detachable with optical fiber interface
End is summarized in the fiber optic bundle of connection, described fiber optic bundle one end by connector connection collection fibers, and the fiber optic bundle other end leads to
Cross connector connection optical fiber probe.
7. quantitative fluorescence analysis instrument according to claim 6, which is characterized in that the coupling assembly includes coaxial arrangement
First convex lens, spike filter and the second convex lens.
8. quantitative fluorescence analysis instrument according to claim 7, which is characterized in that be provided on the test paper anti-for capturing
The detection band of original antibody compound, for being enriched with the control band of free fluorescent marker and Quick Response Code for record number.
9. quantitative fluorescence analysis instrument according to claim 8, which is characterized in that circuit board is additionally provided in the fuselage,
The circuit board include main control chip, filtering and amplifying circuit, fluorescent optical sensor, light source driving circuit and with the control chip
ID chips, power circuit, A/D conversion circuits, time sequence driving circuit, synchronization control circuit and the motor driving electricity being respectively connected with
Road, wherein：
The main control chip connects liquid crystal display, keyboard and thermal printer respectively；
The main control chip passes sequentially through A/D conversion circuits, filtering and amplifying circuit obtains fluorescent optical sensor information；
The fluorescent optical sensor connects the time sequence driving circuit；
The main control chip passes sequentially through synchronization control circuit, light source driving circuit driving light source working；
The main control chip is controlled by clicking driving circuit Driving Stepping Motor；
The main control chip connects the positive and negative anodes in battery case by power circuit.
10. quantitative fluorescence analysis instrument according to claim 9, which is characterized in that the light source includes laser light source, hernia lamp
Light source.
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| CN201810248662.9A CN108181290B (en) | 2018-03-24 | 2018-03-24 | Fluorescent quantitative analyzer |
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| CN201810248662.9A CN108181290B (en) | 2018-03-24 | 2018-03-24 | Fluorescent quantitative analyzer |
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| CN109211865A (en) * | 2018-11-17 | 2019-01-15 | 郑州亲和力科技有限公司 | A kind of quantitative fluorescence analysis instrument |
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| CN111077306A (en) * | 2019-12-30 | 2020-04-28 | 长沙协大生物科技有限公司 | Quantitative detection analyzer for fluorescence immunoassay reagent and detection method thereof |
| CN113640508A (en) * | 2021-07-19 | 2021-11-12 | 安徽桐康医疗科技股份有限公司 | Detection plate for fluorescence immunoassay quantitative analyzer |
| CN114200131A (en) * | 2021-12-13 | 2022-03-18 | 成都云芯医联科技有限公司 | Detection system for luminous characteristics of luminous pearl-like material and preparation method of matched detection test paper |
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| CN114739967A (en) * | 2022-04-15 | 2022-07-12 | 上海健康医学院 | Fluorescence immunoassay quantitative analysis equipment |
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| CN114200131A (en) * | 2021-12-13 | 2022-03-18 | 成都云芯医联科技有限公司 | Detection system for luminous characteristics of luminous pearl-like material and preparation method of matched detection test paper |
| CN114200131B (en) * | 2021-12-13 | 2023-08-15 | 成都云芯医联科技有限公司 | Detection system for luminous characteristics of luminous beads and preparation method of matched detection test paper |
| CN117929345A (en) * | 2024-03-22 | 2024-04-26 | 北京万泰生物药业股份有限公司 | Handheld detection device and fluorescence detection system |
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