CN103808918A - Image-sensor-based detection system of colloidal gold immunoassay - Google Patents
Image-sensor-based detection system of colloidal gold immunoassay Download PDFInfo
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- CN103808918A CN103808918A CN201410040755.4A CN201410040755A CN103808918A CN 103808918 A CN103808918 A CN 103808918A CN 201410040755 A CN201410040755 A CN 201410040755A CN 103808918 A CN103808918 A CN 103808918A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V19/00—Fastening of light sources or lamp holders
- F21V19/001—Fastening of light sources or lamp holders the light sources being semiconductors devices, e.g. LEDs
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V5/00—Refractors for light sources
- F21V5/08—Refractors for light sources producing an asymmetric light distribution
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/46—Measurement of colour; Colour measuring devices, e.g. colorimeters
- G01J3/50—Measurement of colour; Colour measuring devices, e.g. colorimeters using electric radiation detectors
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
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Abstract
The invention discloses an image-sensor-based detection system of colloidal gold immunoassay and belongs to the field of the colloidal gold immunoassay. The detection system is as follows: a test strip placing platform for placing a test strip is arranged on a test strip detection platform; an illumination module and an imaging module are arranged above the test strip placing platform of the test strip detection platform; the illumination module consists of an illumination light source and a light homogenization unit arranged below the illumination light source; the illumination light source is a hollow annular light-emitting diode array light source formed by means that a plurality of light-emitting diodes are uniformly formed in an annular array; an image sensor of the imaging module uses a CMOS image sensor and the signal output end of the imaging module is electrically connected with the input end of a data collecting and processing device. The system adopts the annular matrix illumination and is provided with the light homogenization unit so that output light is homogenized and the detection result is accurate; the imaging module adopts the CMOS image sensor, so that the collection speed is high, and the requirement of dynamically tracking and observing the reaction process can be met.
Description
Technical field
The present invention relates to colloid gold immune analysis and detecting instrument field, particularly relate to and use a kind of instrument that carries out colloid gold immune analysis based on imageing sensor, can be used for the quantitative detection of the colloid gold immune analysis in the field such as Measurement for Biotechnique, food safety detection.
Background technology
The characteristic of utilizing collaurum to combine closely by static with albumen, can be used as label for immunohistochemical reaction, colloid gold immune analysis is in the last few years developed in the research in many fields such as drug test, biomedicine, and be more and more subject to the attention of Related Research Domain, by it and derivative collaurum detection technique has also obtained corresponding development.In recent years, domestic each research institution and company are all competitively devoted to the research of collaurum detecting instrument.
The collaurum detector of producing on market in recent years, for information acquisition and the disposal route of colloidal gold strip, nothing more than two kinds.A kind of for adopting driven by motor linear array photoelectric receiving arrangement to carry out line sweep collection to test strips, then the dot information collecting being sent into microprocessor one by one stores, process again, so the measuring speed of this instrument depends primarily on the speed of motor scanning, relatively slow, and motor scanning mechanism volume is larger; For solving the slow-footed shortcoming of motor scan mode, also have at present a kind of area array CCD that adopts to carry out the collection of taking pictures of face battle array to the information of test strips, then the two-dimensional image information collecting is passed to microprocessor internal and carry out data processing, finally provide the collaurum detector of measurement result.
Although but that the collaurum detector speed of this employing area array CCD is compared the mode of motor scanning is fast, because the quantity of information collecting is larger, after CCD can only wait until that all image informations have all gathered, again electric signal after amplifying, amplifying circuit is transferred to microprocessor, existence is lost time, affect the problem of image acquisition speed, and CCD and additional driving circuit thereof and amplifying circuit volume and the cost of integral device are also increased.Because immunochromatography reaction is a dynamic process, so collaurum colour developing also belongs to dynamic process, and all can not obtain testing result accurately with second-time speed in prior art, and therefore also just cannot realize the tracing observation to course of reaction, cannot more in depth study immunochromatography course of reaction.Even immunohistochemical reaction failure, in the situation that detection speed is slow, the also reason of analytical reactions failure well.In addition, in prior art, for obtaining the homogeneity of illumination, light source is generally only put forward to quantitative requirement, be increased to four even symmetry arrangement of six diodes from two diodes, but only rely on the quantity that increases light source, can not guarantee the homogeneity of illumination, exist because of the even problem that cannot guarantee to collect image accuracy of uneven illumination.
Summary of the invention
The technical problem to be solved in the present invention is to provide the detection system that a kind of colloid gold immune based on imageing sensor is analyzed, can solve existing collaurum detector and can not accurately collect view data with second-time speed, processing speed cannot meet the problem of dynamic tracing observation course of reaction.
For solving the problems of the technologies described above, the invention provides the detection system that a kind of colloid gold immune based on imageing sensor is analyzed, comprising:
ELISA test strip platform, lighting module, image-forming module and data acquisition processing device; Wherein,
Described ELISA test strip platform is provided with the test strips placement platform of placing test strips;
Described lighting module and image-forming module are all arranged on the test strips placement platform top of described ELISA test strip platform;
Described lighting module is made up of lighting source and the light uniformization unit that is arranged on described lighting source below, and wherein said lighting source is to be uniformly distributed the hollow ring light emitting diode matrix light source forming by annular array by multiple light emitting diodes;
The imageing sensor of described image-forming module adopts cmos image sensor, and the signal output part of described image-forming module is electrically connected with the input end of described data acquisition processing device.
Further, in said detecting system, described lighting source is to be uniformly distributed the hollow ring light emitting diode matrix light source forming by annular array by multiple light emitting diodes; Described light uniformization unit is and the hollow ring structure of the form fit of described lighting source.
Further, in said detecting system, it is 525nm that described lighting source adopts 8 wavelength, the angle of divergence is 180 degree, light intensity is 500mcd, and diameter is 3mm light emitting diode, and each light emitting diode adopts and carries out the light emitting diode that shading treatment only has radial light to export;
Described light uniformization unit adopts simultaneously and plates anti-reflection film, another side is hacking face, the homogenising glass plate that centre wavelength is 537nm.
Further, in said detecting system, the imageing sensor front end of described image-forming module is provided with imaging lens group, and described imaging lens group is arranged in the hollow part of described lighting module.
Further, in said detecting system, described data acquisition processing device comprises:
Microprocessor chip, display device, input media, clock chip, USB control chip and USB interface; Wherein,
Described microprocessor chip is electrically connected with described display device, input media, clock chip, USB control chip respectively;
Described USB control chip is electrically connected with described USB interface.
Further, in said detecting system, described display device adopts LCD display, is electrically connected with described microprocessor chip by impact damper.
Further, in said detecting system, described data acquisition processing device also comprises: mini-printer and impact damper two; Described mini-printer is electrically connected with described data acquisition processing device by described impact damper two.
Further, in said detecting system, described data acquisition processing device is electrically connected by the serial line interface of its microprocessor chip and the signal output part of described image-forming module.
Further, in said detecting system, described microprocessor chip adopts DSP microprocessor.
Further, said detecting system also comprises: USB storage, is electrically connected with the USB interface of described data acquisition processing device.
Beneficial effect of the present invention is: the lighting module of this detection system adopts ring quasi array light illumination mode, and is aided with light uniformization unit, makes to export light homogenising more, can be by test strips photograph detected test strips clearer, make testing result more accurate; The imageing sensor adopting due to image-forming module adopts cmos image sensor, and picking rate is faster than CCD, and has automatic gain, Automatic white balance, and dynamic range is large.Cmos image sensor volume is little in addition, low in energy consumption, makes the low in energy consumption and volume of complete machine little.This detection system is simple in structure, volume is little, fast to image acquisition speed when detection, coordinates data acquisition processing device, can meet the requirement of dynamic tracing observation course of reaction.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme of the embodiment of the present invention, below the accompanying drawing of required use during embodiment is described is briefly described, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, do not paying under the prerequisite of creative work, can also obtain other accompanying drawings according to these accompanying drawings.
The structural representation of the detection system that the colloid gold immune based on imageing sensor that Fig. 1 provides for the embodiment of the present invention is analyzed;
The lighting module schematic diagram of the detection system that Fig. 2 provides for the embodiment of the present invention;
The structural representation of the data acquisition processing device of the detection system that Fig. 3 provides for the embodiment of the present invention;
The USB storage of detection system and the schematic diagram of data acquisition processing device coupling part that Fig. 4 provides for the embodiment of the present invention;
The detection system that Fig. 5 provides for the embodiment of the present invention is to dripping the T/C value that records after HCG standard solution and the working curve of raw sample concentration gradient drafting;
The detection system that Fig. 6 provides for the embodiment of the present invention is to dripping the T/C value that records after HBsAg standard solution and the working curve of raw sample concentration gradient drafting.
Embodiment
Below the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Based on embodiments of the invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to protection scope of the present invention.
Figure 1 shows that the detection system that the colloid gold immune based on imageing sensor that the embodiment of the present invention provides is analyzed, this detection system comprises: ELISA test strip platform 1, lighting module 2, image-forming module 3 and data acquisition processing device 4;
Wherein, ELISA test strip platform 1 is provided with the test strips placement platform 101 of placing test strips, can insert a colloidal gold strip 102 to be tested on this test strips placement platform 101 at every turn;
The imageing sensor of image-forming module 3 adopts cmos image sensor 301, and the signal output part of image-forming module 3 is electrically connected with the input end of data acquisition processing device 4.
As shown in Figure 2, the lighting module 2 of said detecting system is made up of lighting source 201 and the light uniformization unit 202 that is arranged on lighting source below; Wherein, lighting source 201 is for being uniformly distributed the hollow ring light emitting diode matrix light source forming by annular array by multiple light emitting diodes; Light uniformization unit 202 is and the hollow ring structure of the form fit of lighting source that light uniformization unit can do further homogenizing processing to the annular light source obtaining, and obtains good uniform light.The lighting module that this hollow ring light emitting diode matrix light source and light uniformization unit matching form, the good uniformity of illumination light, thereby make measurement result accuracy high, the uniform light that the annular array of light sources that multiple light emitting diodes form is sent irradiates on tested paper slip surface, illuminate test strips perform region by diffuse reflection mode, can give prominence to the part that is originally difficult to see clearly, thereby make final measurement result more reliable.The present invention is by the lighting module that adopts the hollow ring light uniformization unit matching of hollow ring light emitting diode matrix light source and setting to form, overcome in prior art, for obtaining the homogeneity of illumination, light source is generally only put forward to quantitative requirement, be increased to four even symmetry arrangement of six diodes from two diodes, increase the quantity of light source and only rely on, and fail to improve illumination uniformity, the part that can not originally be difficult to see clearly according to clear detected test strips, cannot guarantee finally to obtain the problem of view data accurately.
In above-mentioned lighting module 2, it is 525nm that lighting source adopts 8 wavelength, and the angle of divergence is 180 degree, and light intensity is 500mcd, and diameter is 3mm light emitting diode, and each light emitting diode adopts and carries out the light emitting diode that shading treatment only has radial light to export; Described light uniformization unit adopts simultaneously and plates anti-reflection film, another side is hacking face, the homogenising glass plate that centre wavelength is 537nm, and this homogenising glass plate can make light evenly see through.
In said detecting system, image-forming module 3(structure is referring to Fig. 1) cmos image sensor 301 front ends be provided with imaging lens group 302, imaging lens group 302 is arranged in the hollow part of lighting module 2.By the imaging lens group 302 arranging, make under the irradiation of lighting source, after colloidal gold strip 101 information exchanges are crossed imaging lens group and are projected on cmos image sensor 301, light signal is converted into electric signal, after exporting to again data acquisition processing device 4, carry out information extraction and do the analysis of test strips Concentration Algorithm, finally provide measurement result accurately.
As shown in Figure 3, in said detecting system, data acquisition processing device 4 comprises:
In above-mentioned data acquisition processing device 4, display device 404 adopts LCD display, is electrically connected with microprocessor chip 401 by impact damper 402.
Above-mentioned data acquisition processing device 4 also comprises: mini-printer 403 and impact damper 2 4021; Mini-printer 403 is electrically connected with microprocessor chip 401 by impact damper 2 4021.
Above-mentioned data acquisition processing device is electrically connected with the signal output part of image-forming module 3 by the serial line interface 408 of its microprocessor chip 401.
The microprocessor chip of above-mentioned data acquisition processing device 4 adopts DSP microprocessor.
Said detecting system also comprises: USB storage 5, is electrically connected with the USB interface 4070 of data acquisition processing device 4.Coordinate with the USB interface of data acquisition processing device by the USB storage arranging, realize the read and write of the USB storage to peripheral hardware, improve the data space of whole system, can realize the storage of mass data, solve that the speed that serial line interface transmission data exist is slow, efficiency is low, be not easy to process the problem of big data quantity.Existing collaurum detecting instrument detects data for the collaurum recording, often adopt instrument internal memory to store, and memory size is often limited, if amount detection increases, cannot meet the requirement of storage, just must transmit continually by serial line interface and host computer, its process bothers and transfer efficiency is low loses time.In addition, for the view data recording, sometimes need to first store again and analyze and research, but because of the memory capacity of view data often larger, account for very much internal memory, several test strips images just can take internal memory, at this moment relend and help serial line interface to upload, speed is very slow, has affected the efficiency of subsequent detection and detection.And the present invention is by USB control chip and USB interface are set in data acquisition processing device, can connect outside USB storage, the data storage capacity that has well overcome existing collaurum detecting instrument is too little, cannot realize the problem of mass memory.
When said detecting system work, the test strips 102 arranging on ELISA test strip platform 1 is by under lighting module 2 illuminations, on image-forming module 3 after imaging, the electric signal being changed into by image-forming module 3 output images exports data acquisition processing device 4 to, data acquisition processing device 4 to Electric signal processing, analyze after, need the data of storage to export the USB storage 5 being connected with data acquisition processing device 4 to and store.
Below in conjunction with the drawings and specific embodiments, detection system of the present invention is described further.
Fig. 1 is the structural representation that the present invention is based on the detection system of the colloid gold immune analysis of imageing sensor.As seen from Figure 1, this detection system comprises: ELISA test strip platform 1, lighting module 2, image-forming module 3 and data acquisition processing device 4 and USB storage 5;
Wherein, ELISA test strip platform is provided with test strips placement platform 101, and test strips 102 is set on test strips placement platform 101;
Image-forming module 3 is made up of the cmos image sensor 301 as imageing sensor and the imaging lens group 302 that is arranged on cmos image sensor front end.
As shown in Figure 2, the hollow ring light emitting diode matrix light source as lighting source 201 of lighting module 2 is the optical axis symmetry arrangement ringwise around the imaging lens group 302 of image-forming module 3 by multiple light emitting diodes, to obtain annular directional light.
As shown in Figure 3, data acquisition processing device 4 comprises microprocessor chip DSP401, impact damper 402, LCD screen 403, mini-printer 404, keyboard 405, clock chip 406, USB control chip 407 and the serial line interface 408 of communicating by letter with the cmos image sensor 301 of image-forming module 3.
As shown in Figure 4, in this detection system, realize and connect the structure that external USB storer 5 carries out mass data storage and comprise: the USB control chip 407(being connected with microprocessor chip DSP401 is formed by connecting by RAM impact damper 4071 and serial ports transport module 4072) and USB interface 4070(formed USB control module), USB interface 4071 connects outside USB storage 5.
While utilizing detection system of the present invention to detect: by after dripping and having the colloidal gold strip 102 of sample solution to be fixed on test strips placement platform 101, with lighting module 2, the colloidal gold strip 102 on test strips placement platform 101 is thrown light on, adopt the cmos image sensor 301 of the imaging of image-forming module 3 to detect colloidal gold strip 102, obtain the view data of colloidal gold strip 102, then data acquisition processing device 4 carries out the view data of test strips after signal processing, finally obtain dripping in test strips the concentration of sample solution, can be by the external USB storage 5(being connected with data acquisition processing device 4 as USB flash disk) the measurement result data that record are stored.
Test strips placement platform 101 is to design according to the physical dimension of institute's Test paper barrel, finally makes test strips can be embedded on this platform, by exposed being tested surface in the visual field of image-forming module 3.
Selecting of lighting module 2, is first used spectrophotometric to measure the absorption spectrum of colloidal gold solution to be measured, obtains its absorption peak and absorption spectrum width.According to the colloidal gold solution absorption spectrum recording, design diode illumination light source, makes light source be irradiated to spectrum on tested colloid gold label thing in colloidal gold solution absorption spectrum ranges.For obtaining good uniform light illumination, lighting source 201 adopts the multiple light emitting diodes consistent with colloidal gold solution absorption spectrum ranges to carry out annular array and arranges, arrangement is around the symmetrical annular arrangement of described imaging lens group optical axis, and the luminosity of light emitting diode and dispersion angle moderate.In addition, also can adopt professional mechanical technology to carry out trickle polishing to the surface of light emitting diode, to obtain suitable equal light effect, then light uniformization unit 202 is set, to the reduction that decays of parallel light intensity, finally make luminosity more even.
The uniform illumination that lighting module 2 sends is to after on colloidal gold strip 101, every some meeting of this colloidal gold strip 101 has absorption in various degree to light, the light that each point sends can be by being hunted down after imaging lens group 302 on the light sensitive diode of cmos image sensor 301, and producing output current, the intensity of its electric current is directly proportional to the intensity information of colloidal gold strip 101 epigraphs.According to Beer-Lambert Law, the size of this signal is directly proportional to the concentration of solution on colloidal gold strip just.This electric signal is again after the amplifier in cmos image sensor 301, be directly changed into corresponding digital signal by the A/D amplifier in cmos image sensor 301 again, digital signal is directly delivered data acquisition processing device 4 microprocessor chip DSP401 after merging carries out picture signal processing.
The microprocessor chip DSP401 of data acquisition processing device 5 is as the core of whole system data acquisition and signal processing, main by receiving the order from keyboard 405, command each peripheral hardware to carry out corresponding function, as can be adopted LCD screen at display device 404() on the printing, data information acquisition, the data of carrying out colloidal gold strip 101 by serial line interface 408 of displaying contents, mini-printer 403 can be transferred to USB storage 5 etc. by USB control chip 407.
The storage detailed process of utilizing USB storage to carry out mass data is: first, under the control of microprocessor chip DSP401, collected the view data of colloidal gold strip 101 by cmos image sensor 301, the laggard row data-signal of internal memory that deposits microprocessor chip DSP401 in is processed and is calculated, its result exists in the internal memory of DSP401, when capacity is increased to a certain degree, be that the internal memory of DSP401 is can not meet memory requirement time, DSP401 will select calculation result data first to pass through the RAM impact damper 4071 of USB control chip in the mode of bulk transfer, finally enter into USB storage 5 by serial ports transport module 4072 and USB interface.If in measuring process, view data is had to query, can by adopt view data be saved in USB storage 5, then by USB storage 5, they are passed to PC and carry out data processing.
The course of work of detection system of the present invention is as follows:
After this detection system start powers on, display device 404(LCD screen) demonstration user information, setting sample kind by the input of keyboard 405 is No. ID, sample, tested project, operator, yin and yang attribute reference value, store, print and check testing result, can revise time, startup " detection " order etc. of system simultaneously.
Testing sample solution is splashed on colloidal gold strip 102, after being chromogenic reaction after antigen in sample solution is fully combined with the antibody of colloidal gold strip 102, colloidal gold strip 102 is placed on test strips placement platform 101.Lighting source 201(is hollow diode annular array light source), the parallel beam sending is behind light uniformization unit 202, the hot spot forming on colloidal gold strip 102 surfaces can be to cover the tested region of whole test strips, and illumination is even, to guarantee that the present invention has higher stability and sensitivity.On colloidal gold strip 102, have two bands, whether one its objective is in order to check test strips qualified for monitoring band (C band), and the sample solution of this band is changeless in conjunction with concentration; Another one is tested measuring tape (T band), and the colour developing degree of this band depends on the concentration of sample solution, finally gets the concentration value that T/C is final sample.
" detection " key on keypad 405, starts image acquisition action.The detected district of colloidal gold strip 102 has absorbed after the light from lighting module 2, be subject to the commander of microprocessor chip DSP401, by imaging lens group 302 imaging on the photo-sensitive cell of cmos image sensor 301 of image-forming module 2, the image light intensity information of colloidal gold strip 102 is converted into electric signal, its size is directly proportional to the size of absorbance, after the electric image signal that repeatedly imaging obtains is gathered by microprocessor chip DSP401 again, depositing the laggard row operation of internal data memory in averages, obtain the data of piece image, carried out after filtering processing, carry out again after interpolation processing, image in lcd screen (being display device 404), then utilize mean value view data to carry out data processing, at display device 404(LCD screen) on draw in real time the upwards absorbance curve of each point of one-dimensional square in test strips using X, Y as horizontal, ordinate respectively, with the curve of T band and C information, pass through again detection algorithm, obtain the value of T/C, the i.e. concentration of this sample solution, and then biomolecule content to be measured contained in sample solution is made to judgement accurately, this value also can compare with the yin and yang attribute reference value of corresponding kind, to obtain yin and yang attribute judgement accurately.This judged result is admitted to internal data memory storage, testing result can be exported by USB storage 5.Meanwhile, in the process of acquisition of image data, can pass through button operation, the large capacity image data of collection is deposited in USB storage 5 by USB controller 407, for PC reconstructed image, contribute to further research.
Embodiment:
As shown in Figure 1, lighting source 201 in lighting module 2, adopting 8 wavelength is 525nm, the angle of divergence is 180 degree, and light intensity is 500mcd, and diameter is 3mm light emitting diode, circular array is arranged, on each diode, carry out again shading treatment, make it only have radial light output, then by light emitting diode around the symmetrical annular arrangement of described imaging lens group optical axis.Light uniformization unit 202 adopts homogenising glass plate, and its one side is plated anti-reflection film, and centre wavelength is 537nm, make light fully see through and go, another side hacking, scattering is abundant, and be placed on light emitting diode matrix 201 belows, therefore in test strips 102, can obtain the light of brightness and even intensity.
The OV528 serial line interface transmitting image sensor assembly that the cmos image sensor 301 of image-forming module 3 can adopt OmniVision company of the U.S. to produce, resolution VGA is 640 × 480, transmission speed is 115200bit/s, camera lens is 1/4 "; directly export uncompressed form output; reduce the time waste of compression reconciliation pressure zone, this cmos image sensor is less than CCD volume, low in energy consumption.By adopting Novel CMOS picture sensor, its image acquisition speed is faster than CCD, and has automatic gain, Automatic white balance, and dynamic range is large, can effectively improve detection efficiency.
Standard model I: employing concentration is the HCG of 400mIU/ml, 200mIU/ml, 100mIU/ml, 50mIU/ml, 25mIU/ml.
Standard model II: concentration content is the HBsAg of 100%, 50%, 25%, 12.5%, 6.25% 100ng/ml.
The dsp chip MC56F8366 that the microprocessor chip DSP401 of data acquisition processing device 4 can adopt Freescale company to produce, is Harvard structure, supports parallel processing, and dominant frequency 60MHz reaches the execution speed of 60,000,000 instructions; Adopt multiple pipeline organization, can complete simultaneously and read instruction, Instruction decoding, several stages such as instruction execution; In sheet, there is the program flash memory of 512K, the program storage of 4K, the data flash memory of 8K, the data-carrier store of 32K; The modulus converter A/D of 16 12, dual serial communication interface (SCIs), contains 16 position datawires and address wire, also has in addition a large amount of I/O mouths, is beneficial to the expansion of chip.Carry out view data processing by the DSP microprocessor that adopts processing speed to be exceedingly fast, with obtain speed image and coordinate faster than the cmos image sensor of CCD, so whole data acquisition and conversion speed are faster than prior art, and the height of dsp chip is integrated, also save many chips that extend out, further improved the integrated level of complete machine.
The SL811HST that USB control chip 407 can adopt CYPRESS company of the U.S. to produce, USB storage 5 can adopt 128M Netac USB flash disk, and USB1.1 host-host protocol also can adopt the larger USB storage of capacity of USB2.0 or USB3.0 host-host protocol.Under the commander of DSP401, USB flash disk 5 is carried out to batch write operation, USB flash disk 5 adopts and can make the communication speed between dsp chip 401 and USB flash disk 5 can reach 150kbit/s, finally realizes measurement result mass memory.
It was 6 seconds that the detection system of the present embodiment completes one-time detection required time, was better than the detection speed of the collaurum detector of existing CCD.
As shown in Figure 5,6, for dripping after HCG and two kinds of standard solution series of HBsAg, working curve with the T/C value recording and the drafting of raw sample concentration gradient, wherein, horizontal ordinate is T/C value, ordinate is raw sample concentration gradient, and the CV value of two kinds of normal concentration solution all, more than 95%, is better than prior art.
This detection system is by all components and parts together with the system integration, and complete machine is integrated, and volume is little, its volume is wide 195mm × dark 185mm × high 115mm, weight is less than 1.6kg, and volume and weight is all better than the collaurum detector of existing CCD, can be carried to field and carry out field quick detection.
The colloid gold immune analyzing and testing system based on cmos image sensor of the embodiment of the present invention, for the color feature of collaurum, adopt multiple light emitting diodes to distribute ringwise, be aided with again light uniformization unit illumination is carried out to homogenising processing, reduce the specific lighting source of light source ground unrest of system, and adopt cmos image sensor to coordinate as image receiving element and with the data acquisition processing device that adopts microprocessor chip DSP, improve detection speed, reduce power consumption, dwindled volume.Data storage adopts the data acquisition processing device control USB chip that DSP microprocessor forms in addition, can connect outside high capacity U disc, realize quick mass data storage, avoided the slow transmission speed of original little internal memory and serial line interface and host computer slow, inefficient problem.
The above; only for preferably embodiment of the present invention, but protection scope of the present invention is not limited to this, is anyly familiar with in technical scope that those skilled in the art disclose in the present invention; the variation that can expect easily or replacement, within all should being encompassed in protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.
Claims (10)
1. the detection system that the colloid gold immune based on imageing sensor is analyzed, is characterized in that, comprising:
ELISA test strip platform, lighting module, image-forming module and data acquisition processing device; Wherein,
Described ELISA test strip platform is provided with the test strips placement platform of placing test strips;
Described lighting module and image-forming module are all arranged on the test strips placement platform top of described ELISA test strip platform;
Described lighting module is made up of lighting source and the light uniformization unit that is arranged on described lighting source below, and wherein said lighting source is to be uniformly distributed the hollow ring light emitting diode matrix light source forming by annular array by multiple light emitting diodes;
The imageing sensor of described image-forming module adopts cmos image sensor, and the signal output part of described image-forming module is electrically connected with the input end of described data acquisition processing device.
2. the detection system that the colloid gold immune based on imageing sensor according to claim 1 is analyzed, is characterized in that, described light uniformization unit is and the hollow ring structure of the form fit of described lighting source.
3. the detection system that the colloid gold immune based on imageing sensor according to claim 2 is analyzed, it is characterized in that, it is 525nm that described lighting source adopts 8 wavelength, the angle of divergence is 180 degree, light intensity is 500mcd, diameter is 3mm light emitting diode, and each light emitting diode adopts and carries out the light emitting diode that shading treatment only has radial light to export;
Described light uniformization unit adopts simultaneously and plates anti-reflection film, another side is hacking face, the homogenising glass plate that centre wavelength is 537nm.
4. the detection system that the colloid gold immune based on imageing sensor according to claim 1 and 2 is analyzed, it is characterized in that, the imageing sensor front end of described image-forming module is provided with imaging lens group, and described imaging lens group is arranged in the hollow part of described lighting module.
5. the detection system that the colloid gold immune based on imageing sensor according to claim 1 is analyzed, is characterized in that, described data acquisition processing device comprises:
Microprocessor chip, display device, input media, clock chip, USB control chip and USB interface; Wherein,
Described microprocessor chip is electrically connected with described display device, input media, clock chip, USB control chip respectively;
Described USB control chip is electrically connected with described USB interface.
6. the detection system that the colloid gold immune based on imageing sensor according to claim 5 is analyzed, is characterized in that, described display device adopts LCD display, is electrically connected with described microprocessor chip by impact damper.
7. the detection system that the colloid gold immune based on imageing sensor according to claim 5 is analyzed, is characterized in that, described data acquisition processing device also comprises: mini-printer and impact damper two; Described mini-printer is electrically connected with described data acquisition processing device by described impact damper two.
8. the detection system that the colloid gold immune based on imageing sensor according to claim 5 is analyzed, is characterized in that, described data acquisition processing device is electrically connected by the serial line interface of its microprocessor chip and the signal output part of described image-forming module.
9. the detection system of analyzing according to the colloid gold immune based on imageing sensor described in claim 5 or 8, is characterized in that, described microprocessor chip adopts DSP microprocessor.
10. the detection system that the colloid gold immune based on imageing sensor is analyzed according to claim 1 or 5, is characterized in that, also comprises: USB storage, is electrically connected with the USB interface of described data acquisition processing device.
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