CN104836942A - Colloidal gold portable CCD readout device - Google Patents
Colloidal gold portable CCD readout device Download PDFInfo
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- CN104836942A CN104836942A CN201410609731.6A CN201410609731A CN104836942A CN 104836942 A CN104836942 A CN 104836942A CN 201410609731 A CN201410609731 A CN 201410609731A CN 104836942 A CN104836942 A CN 104836942A
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Abstract
The invention relates to a colloidal gold readout device, especially relates to a colloidal gold portable CCD readout device comprising an upper cover, a lower cover, a front upper cover, an image acquisition device, a display unit, a processing system, and a power supply module. The upper cover and the lower cover are fixedly connected together by a screw, and the left upper part of the upper cover is provided with a notch, in which is disposed the image acquisition device. The upper part of the notch is provided with a closed front upper cover, and the upper cover is provided with an inserting opening, and samples can be disposed in the image acquisition device through the inserting opening. The right upper part of the upper cover is provided with an inclined surface, which is provided with a button module, and a transparent window is disposed on the button module. The display unit is disposed on the lower part of the transparent window, and the lower cover disposed on a lower part of an installation opening is fixedly provided with a processing system, which is respectively connected with the image acquisition device, the button module, the display unit, and the power supply module. The readout device has advantages of small size, light weight, portability, and abilities of processing data independently, and realizing quantitative detection function.
Description
Technical field
The present invention relates to a kind of collaurum readout instrument, particularly the portable CCD readout instrument of a kind of collaurum.
Background technology
Test paper detecting method is a kind of conventional detection method that medical science, medicine, food and environmental area detect biochemistry amount.Colloid gold label (be called for short gold mark) test paper is a kind of common detection test paper, and it can the multiple biochemistry amount such as qualitative detection clenobuterol hydrochloride, Ractopamine.When the content of analysans in sample is higher than detectability, test paper does not develop the color, and when the content of analysans in sample is lower than detectability, the sample of variable concentrations will present deep mixed color on test paper.Because detection paper is qualitative detection, can not testing requirement be met when the many and accuracy of detection of sample size requires high, therefore develop a kind of biochemistry detection instrument that quantitatively can detect concentration of specimens very necessary.
Chinese invention patent application (application number: 201110115964.7 applyings date: 2011-05-06) discloses a kind of CCD of employing Digital image technology and immediately takes detected target, processes and a kind of CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers of reading and analytical method, is made up of image acquisition device, data processor, result follower; Motherboard handshake terminal in data processor carries out bidirectional data exchange with the industrial camera in image procossing and reading software and image acquisition device respectively.The detection method of this patent is as follows: light is sent by LED illuminating chamber, be radiated in reacted test strips, after test strips surface diffuse reflectance, light reflexes to industrial camera by optical flat mirror, industrial camera shooting generates digital picture, then be sent to computer software and carry out analyzing and processing, finally obtain the reading numerical value of p-wire on colloidal gold immunochromatographimethod diagnose test paper and nature controlling line.
The most of volume of biochemistry detection instrument common is on the market comparatively large, not easily portable, is suitable for carrying out accurate measurement in laboratory.And majority can not independent process data, need the aids such as computer to measure.When needing the fast detecting of carrying out great amount of samples at the scene, or when not having aid to need independent detection, these large-scale instruments are just inapplicable.
Summary of the invention
In order to solve above-mentioned technical problem, the object of this invention is to provide the portable CCD readout instrument of a kind of collaurum, this readout instrument has little, lightweight, the portable feature of volume, can independent process data, reaches the function quantitatively detected.
In order to realize above-mentioned object, present invention employs following technical scheme:
The portable CCD readout instrument of collaurum, this readout instrument comprises upper cover, lower cover, front upper cover, image collecting device, display unit, treatment system and power module; Described upper cover and lower cover are fixed by screw, recess is provided with above the left side of upper cover, described image collecting device is set in recess, described front upper cover is set above recess and closes, and in upper cover, be provided with the insert port entering image collecting device for sample; Above the right side of upper cover, inclined-plane is set, inclined-plane is provided with key-press module, and transparent window is provided with on key-press module, described display unit is arranged on below transparent window, lower cover below installing port is fixedly installed described treatment system, and treatment system connects described image collecting device, key-press module, display unit and power module respectively; Described image collecting device comprises card bed, upper clamp slice seat, compressing tablet, CCD seat, CCD camera and illuminating source; The front end of described card bed is provided with the groove of opening, and the top that upper clamp slice seat is arranged on groove forms sample cell, and sample cell and described insert port connect, and the top of sample cell is uncovered, and the rear end of sample cell arranges described compressing tablet; The rear end of described card bed is provided with holddown groove, described CCD seat is " Z " font, and the lower end of CCD seat is fixedly installed in holddown groove, and upper end is provided with CCD installing port, described CCD camera is mounted in CCD installing port, and the camera lens of CCD camera is just to sample cell; The outer side-lower of described CCD installing port is provided with joint pin, and described illuminating source is fixedly installed on joint pin, is provided with the mesopore passed for light or camera lens in the middle part of illuminating source; Described treatment system comprises power module, Computer Vision module, single-chip microcomputer, FPGA unit, video memory and DSP video collector; Described image collecting device connects Computer Vision module, Computer Vision model calling FPGA unit, and FPGA unit connects DSP video collector, and DSP video collector connects video memory; Described Computer Vision module, FPGA unit, key-press module are all connected with single-chip microcomputer with display unit; Described Computer Vision module adopts SAA7114 Video Quality Metric chip, and single-chip microcomputer adopts ATMEGA64 single-chip microcomputer, and video memory adopts two panels 44 pin IS51LV25616 chip, and FPGA unit is the EEPROM that a slice stores the program of FPGA.
As further improvement, described compressing tablet is elastic sheet metal, and front end is provided with downward pressure mouth, and rear end is fixedly installed on CCD seat by screw.
As further improvement, described key-press module adopts membrane switch assemblies.
As further improvement, described SAA7114 Video Quality Metric chip converts the vision signal of standard to 24 position digital signals, is divided into brightness signal Y, color difference signal U and V to export; Extract the line synchronizing signal, the field sync signal that comprise the positional information of pixel in digital picture, for FPGA unit synthetic image memory address.
As further improvement, described SAA7114 Video Quality Metric chip internal has 25 eight bit registers, by its mode of operation of iic bus interface selection able to programme, when the IIC SA pin ground connection of SAA7114 Video Quality Metric chip, it is 49H that IIC reads address, and write address is 48H; Adopt ATMEGA64 single-chip simulation iic bus agreement to 25 register programmings of SAA7114 Video Quality Metric chip; P3.4 pin, the P3.5 pin of ATMEGA64 single-chip microcomputer 2 are connected with SCL, SDA of SAA7114 respectively, and Simulation with I IC bus is 3.3V and ATMEGA64 single-chip microcomputer 2 for the numerical portion supply power voltage of SAA7114 Video Quality Metric chip is 5V, and level is compatible.
As further improvement, described DSP video collector, makes FPGA unit gather one-frame video data by GPIO4, send interrupt signal to DSP video collector after FPGA unit completes by INT4, DSP video collector reads the data in SRAM again, gives single-chip microcomputer after process result.
The present invention, owing to adopting above-mentioned technical scheme, first inserts standard color card Criterion curve, is stored in the processor of single chip computer in instrument cpu motherboard.8 sections of grey compositions of standard color card standard from shallow to deep, after the IMAQ of 8 segment standard values, are generated the color curve of internal standard, correct the view data gathered, make test value more accurate by single-chip microcomputer process.When again measuring, inputting the test paper picture that a width collects and just can automatically calculate gray feature value, and obtaining concentration of specimens to be detected accordingly according to the functional relation preset.When carrying out detection paper, test card is inserted sample cell, adopt CCD camera collection test paper image, carry out image analysis processing by image processing module, the data after process are imported in single-chip microcomputer, compare with calibration curve, draw surveyed concentration, then exported by display screen.The integrated image acquisition process of instrument own and output, divorced from computer.The man-machine interface of instrument has button operation, can carry out relevant setting and read final data by display screen.Instrument structure itself is light, is applicable to carrying with, and it is relatively more accurate to detect data, overcomes the problem that large-scale detecting instrument heaviness is inconvenient to movement.The composition module required device of instrument is more common, and price comparison is low, makes the overall price of instrument lower, accords with the demands of the market.
Accompanying drawing explanation
Fig. 1, Fig. 2 are structural representation of the present invention.
Fig. 3 is the structural representation of upper cover.
Fig. 4 is the structural representation of image collecting device.
Fig. 5 is the structured flowchart for the treatment of system.
Fig. 6 ~ Fig. 7 is the circuit structure diagram of single-chip microcomputer.
Fig. 8 is the circuit structure diagram of power supply.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is made a detailed explanation.
The portable CCD readout instrument of collaurum as shown in Figure 1 and Figure 2, this readout instrument comprises upper cover 1, lower cover 2, front upper cover 3, image collecting device 4, display unit 6, treatment system 5 and power module 8; Described upper cover 1 and lower cover 2 are fixed by screw.As shown in Figure 3, above the left side of upper cover 1, be provided with recess 11, described image collecting device 4 is set in recess 11, described front upper cover 3 is set above recess 11 and closes, and in upper cover 1, be provided with the insert port 12 entering image collecting device 4 for sample 10; Inclined-plane 13 is set above the right side of upper cover 1, inclined-plane 13 is provided with membrane switch assemblies 7, and transparent window is provided with in membrane switch assemblies 7, described display unit 6 is arranged on below transparent window, lower cover 2 below installing port is fixedly installed described treatment system 5, and treatment system 5 connects described image collecting device 4, membrane switch assemblies 7, display unit 6 and power module 8 respectively.
As shown in Figure 4, described image collecting device 4 comprises card bed 41, upper clamp slice seat 43, compressing tablet 44, CCD seat 42, CCD camera 45 and illuminating source 46; The front end of described card bed 41 is provided with the groove 412 of opening, the top that upper clamp slice seat 43 is arranged on groove 412 forms sample cell 48, sample cell 48 and described insert port 12 connect, and the top of sample cell 48 is uncovered, and the rear end of sample cell 48 arranges described compressing tablet 44; Compressing tablet 44 is elastic sheet metal, and front end is provided with downward pressure mouth, and rear end is fixedly installed on CCD seat 42 by screw.The rear end of described card bed 41 is provided with holddown groove 411, described CCD seat 42 is " Z " font, the lower end of CCD seat 42 is fixedly installed in holddown groove 411, upper end is provided with CCD installing port 421, described CCD camera 45 is mounted in CCD installing port 421, and the camera lens of CCD camera 45 is just to sample cell 48; The outer side-lower of described CCD installing port 421 is provided with joint pin 47, and described illuminating source 46 is fixedly installed on joint pin 47, is provided with the mesopore passed for light or camera lens in the middle part of illuminating source 46.
As shown in Figure 5, described treatment system 5 comprises power module, Computer Vision module, single-chip microcomputer, FPGA unit, video memory and DSP video collector; Described Computer Vision module adopts SAA7114 Video Quality Metric chip, and single-chip microcomputer adopts ATMEGA64 single-chip microcomputer.Described CCD camera 45 connects SAA7114 Video Quality Metric chip, and SAA7114 Video Quality Metric chip connects FPGA unit, and FPGA unit connects DSP video collector, and DSP video collector connects video memory; Described Computer Vision module, FPGA unit, membrane switch assemblies 7 are all connected with single-chip microcomputer with display unit 6.Described SAA7114 Video Quality Metric chip converts the vision signal of standard to 24 position digital signals, is divided into brightness signal Y, color difference signal U and V to export; Extract the line synchronizing signal, the field sync signal that comprise the positional information of pixel in digital picture, for FPGA unit synthetic image memory address.SAA7114 Video Quality Metric chip internal has 25 eight bit registers, and by its mode of operation of iic bus interface selection able to programme, when the IIC SA pin ground connection of SAA7114 Video Quality Metric chip, it is 49H that IIC reads address, and write address is 48H; Adopt ATMEGA64 single-chip simulation iic bus agreement to 25 register programmings of SAA7114 Video Quality Metric chip; P3.4 pin, the P3.5 pin of ATMEGA64 single-chip microcomputer 2 are connected with SCL, SDA of SAA7114 respectively, and Simulation with I IC bus is 3.3V and ATMEGA64 single-chip microcomputer 2 for the numerical portion supply power voltage of SAA7114 Video Quality Metric chip is 5V, and level is compatible.For this reason on SCL and SDL transmission line without the need to adding two-way level converting chip, iic bus still can transmit reliably.
Two panels 44 pin IS51LV25616 (1M SRAM) selected by video memory, the two field picture come with storage of collected.Owing to adopting the fpga chip based on SRAM look-up table configuration in system, its program power down is volatile, all needs to load FPGA program after each start.So system needs a slice EEPROM to be used for storing the program of FPGA.
SAA7114 Video Quality Metric chip capable lock clock signal is the data crystal oscillator clock of 27MHZ, DSP is 10MHZ.If it is infeasible for therefore directly receiving the picture signal after conversion with DSP, need collection and the storage of special module controls image.In image acquisition phase, module will be given SAA7114 the data wire of SRAM and be generated write signal and the corresponding pixel memory address in sram of SRAM according to the clock sync signal that SAA7114 exports, and data wire, address wire and the read-write control line again SRAM after having gathered transfers to DSP to control.DSP video collector gathers one-frame video data by the enable FPGA of GPIO4, send interrupt signal to read data in SRAM again to DSP, DSP, give single-chip microcomputer after process result after FPGA completes by INT4.Single-chip microcomputer is by the analysis to data, and contrast standard curve, draws final data.Then single-chip microcomputer is by display unit 6 output processing result, and key-press module operates as man-machine interface.
In order to verify feasibility and the practicality of design system, the Ractopamine standard solution of this design employing 10 kinds of variable concentrations is as detection sample.
At room temperature, 3 0ng/ml, 0.5ng/ml, 1.0ng/ml, 1.5ng/ml, 2.0ng/ml, 2.5ng/ml, 3.0ng/ml, 3.5ng/ml, 4.0ng/ml, 4.5ng/ml standard items are dripped respectively in the detect aperture of every bar gold test strip, after at room temperature reacting 5min, test card is inserted in sample 10 magazine, read measurement result with instrument, measurement result and standard value are compared as follows shown in table:
As can be seen from experimental result:
The measured value that experiment obtains is more basically identical with standard value, has a little bias, achieves the quantitative measuring ability of expection.
Claims (6)
1. the portable CCD readout instrument of collaurum, is characterized in that: this readout instrument comprises upper cover (1), lower cover (2), front upper cover (3), image collecting device (4), display unit (6), treatment system (5) and power module (8); Described upper cover (1) and lower cover (2) are fixed by screw, recess (11) is provided with above the left side of upper cover (1), described image collecting device (4) is set in recess (11), recess (11) top arranges described front upper cover (3) and closes, and in upper cover (1), be provided with the insert port (12) entering image collecting device (4) for sample; Inclined-plane (13) is set above the right side of upper cover (1), inclined-plane (13) are provided with key-press module (7), and transparent window is provided with on key-press module (7), described display unit (6) is arranged on below transparent window, lower cover (2) below installing port is fixedly installed described treatment system (5), and treatment system (5) connects described image collecting device (4), key-press module (7), display unit (6) and power module (8) respectively; Described image collecting device (4) comprises card bed (41), upper clamp slice seat (43), compressing tablet (44), CCD seat (42), CCD camera (45) and illuminating source (46) (46); The front end of described card bed (41) is provided with the groove (412) of opening, the top that upper clamp slice seat (43) is arranged on groove (412) forms sample cell (48), sample cell (48) and described insert port (12) connect, the top of sample cell (48) is uncovered, and the rear end of sample cell (48) arranges described compressing tablet (44); The rear end of described card bed (41) is provided with holddown groove (411), described CCD seat (42) is " Z " font, the lower end of CCD seat (42) is fixedly installed in holddown groove (411), upper end is provided with CCD installing port (421), described CCD camera (45) is mounted in CCD installing port (421), and the camera lens of CCD camera (45) is just to sample cell (48); The outer side-lower of described CCD installing port (421) is provided with joint pin (47), and described illuminating source (46) is fixedly installed on joint pin (47), and illuminating source (46) middle part is provided with the mesopore passed for light or camera lens; Described treatment system (5) comprises power module, Computer Vision module, single-chip microcomputer, FPGA unit, video memory and DSP video collector; Described image collecting device (4) connects Computer Vision module, Computer Vision model calling FPGA unit, and FPGA unit connects DSP video collector, and DSP video collector connects video memory; Described Computer Vision module, FPGA unit, key-press module (7) are all connected with single-chip microcomputer with display unit (6); Described Computer Vision module adopts SAA7114 Video Quality Metric chip, and single-chip microcomputer adopts ATMEGA64 single-chip microcomputer, and video memory adopts two panels 44 pin IS51LV25616 chip, and FPGA unit is the EEPROM that a slice stores the program of FPGA.
2. the portable CCD readout instrument of collaurum according to claim 1, it is characterized in that: compressing tablet (44) is elastic sheet metal, front end is provided with downward pressure mouth, and rear end is fixedly installed on CCD seat (42) by screw.
3. the portable CCD readout instrument of collaurum according to claim 1, is characterized in that: key-press module (7) adopts membrane switch assemblies.
4. the portable CCD readout instrument of collaurum according to claim 1, is characterized in that: SAA7114 Video Quality Metric chip converts the vision signal of standard to 24 position digital signals, is divided into brightness signal Y, color difference signal U and V to export; Extract the line synchronizing signal, the field sync signal that comprise the positional information of pixel in digital picture, for FPGA unit synthetic image memory address.
5. the portable CCD readout instrument of collaurum according to claim 1, it is characterized in that: SAA7114 Video Quality Metric chip internal has 25 eight bit registers, by its mode of operation of iic bus interface selection able to programme, when the IIC SA pin ground connection of SAA7114 Video Quality Metric chip, it is 49H that IIC reads address, and write address is 48H; Adopt ATMEGA64 single-chip simulation iic bus agreement to 25 register programmings of SAA7114 Video Quality Metric chip; P3.4 pin, the P3.5 pin of ATMEGA64 single-chip microcomputer 2 are connected with SCL, SDA of SAA7114 respectively, and Simulation with I IC bus is 3.3V and ATMEGA64 single-chip microcomputer 2 for the numerical portion supply power voltage of SAA7114 Video Quality Metric chip is 5V, and level is compatible.
6. the portable CCD readout instrument of collaurum according to claim 1, it is characterized in that: DSP video collector, FPGA unit is made to gather one-frame video data by GPIO4, send interrupt signal to DSP video collector by INT4 after FPGA unit completes, DSP video collector reads the data in SRAM again, gives single-chip microcomputer after process result.
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| CN109490299A (en) * | 2018-09-04 | 2019-03-19 | 广东朗源生物科技有限公司 | A kind of portable mycotoxin colloidal gold detector and application method |
| CN110231295A (en) * | 2019-06-28 | 2019-09-13 | 中国计量大学 | A kind of image acquisition and processing system of portable colloidal gold CCD readout instrument |
| CN111551731A (en) * | 2020-05-25 | 2020-08-18 | 山西瑞豪生物科技有限公司 | Intelligent colloidal gold biomedical detection method |
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